Publications by authors named "Wen-Feng Liu"

28 Publications

  • Page 1 of 1

Design of a High-Efficiency and -Gain Antenna Using Novel Low-Loss, Temperature-Stable LiTi-(CuNb)O Microwave Dielectric Ceramics.

ACS Appl Mater Interfaces 2021 Jan 24;13(1):912-923. Epub 2020 Dec 24.

International Research Centre for Renewable Energy, State Key Laboratory of Multiphase Flow in Power Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.

Microwave dielectric ceramics are vital for filters, dielectric resonators, and dielectric antennas in the 5G era. It was found that the (CuNb) substitution can effectively adjust the TCF (temperature coefficient of resonant frequency) of LiTiO and simultaneously increase its × ( and denote the quality factor and the resonant frequency, respectively) value. Notably, excellent microwave dielectric properties (ε (permittivity) ≈ 18.3, × ≈ 77,840 GHz, and TCF ≈ +9.8 ppm/°C) were achieved in the LiTi(CuNb)O (LTCN) ceramic sintered at 1140 °C. Additionally, the sintering temperature of LTCN was reduced to 860 °C by the addition of 3 wt % HBO, exhibiting superior microwave dielectric properties ( ≈ 21.0, × ≈ 51,940 GHz, and TCF ≈ 1.4 ppm/°C) and being chemically compatible with silver. Moreover, LTCN + 3 wt % HBO ceramics were designed as a patch antenna and a dielectric resonator antenna, both of which showed high simulated radiation efficiencies (88.4 and 93%) and gains (4.1 and 4.03 dBi) at the center frequencies (2.49 and 10.19 GHz). The LTCN + 3 wt % HBO materials have promising future application for either 5G mobile communication devices and/or in low-temperature co-fired ceramic technology owing to their high , low sintering temperature, small density, and good temperature stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c18836DOI Listing
January 2021

Synergistic anti-inflammatory effects of silibinin and thymol combination on LPS-induced RAW264.7 cells by inhibition of NF-κB and MAPK activation.

Phytomedicine 2020 Nov 19;78:153309. Epub 2020 Aug 19.

Department of Pharmacology, School of Basic Medical Science, Lanzhou University, Lanzhou 730000, P. R. China. Electronic address:

Background: Combination drug therapy has become an effective strategy for inflammation control. The anti‑inflammatory capacities of silibinin and thymol have each been investigated on its own, but little is known about the synergistic anti-inflammatory effects of these two compounds.

Purpose: This study aims to investigate the synergistic anti-inflammatory effects of silibinin and thymol when administered in combination to lipopolysaccharide (LPS)-induced RAW264.7 cells.

Methods: RAW264.7 cells were pre-treated with silibinin and thymol individually or in combination for 2 h before LPS stimulation. Cell viability was detected by the MTT assay. Nitric oxide (NO) production was measured by Griess reagent. Reactive oxygen species (ROS) was evaluated by 2',7'-dichlorofluorescein-diacetate. ELISA was used to detect tumour necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Western blot was performed to analyse the protein expression of LPS-induced RAW264.7 cells.

Results: We observed a synergistic anti-inflammatory effect of silibinin and thymol when administered in combination to LPS-induced RAW264.7 cells. Silibinin combined with thymol (40 μM and 120 μM respectively, with the molar ratio 1:3) had more potent effects on the inhibition of NO, TNF-α, and IL-6 than those exerted by individual administration of these compounds in LPS-induced RAW264.7 cells. The combination of silibinin and thymol (40 μM and 120 μM respectively, with the molar ratio 1:3) strongly inhibited ROS and cyclooxygenase-2 (COX-2). More importantly, the combination of silibinin and thymol (40 μM and 120 μM respectively, with the molar ratio 1:3) was also successful in inhibiting nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) activities. Our results suggest that the synergistic anti-inflammatory effects of silibinin with thymol were associated with the inhibition of NF-κB and MAPK signalling pathways.

Conclusion: The combination of silibinin and thymol (40 μM and 120 μM, respectively, with the molar ratio 1:3) could inhibit inflammation by suppressing NF-κB and MAPK signalling pathways in LPS-induced RAW264.7 cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2020.153309DOI Listing
November 2020

Assessment of Serum Tumor Markers for Predicting Ocular Metastasis in Lung Adenocarcinoma: A Retrospective Study.

Dis Markers 2020 25;2020:2102158. Epub 2020 Jun 25.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute, Nanchang, 330006 Jiangxi, China.

The purpose of this study was to detect clinical variations between lung adenocarcinoma patients with and without ocular metastasis (OM) to identify risk factors for OM and assess the diagnostic values. We included 1153 patients with lung adenocarcinoma in this study. Independent -tests and chi-square tests were used to compare patients' clinical characteristics. Statistically significant parameters were analyzed by binary logistic regression to detect risk factors of OM. The results showed that the OM group had increased alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), cytokeratin fragment 19 (CYFRA 21-1), carbohydrate antigen- (CA-) 125, CA-153, and total prostate-specific antigen (TPSA) compared with the NOM group. CYFRA21-1 is the most useful biomarker for detecting OM in this population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/2102158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334773PMC
June 2021

An ultra-broadband terahertz metamaterial coherent absorber using multilayer electric ring resonator structures based on anti-reflection coating.

Nanoscale 2020 May 23;12(17):9769-9775. Epub 2020 Apr 23.

Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049, China.

We propose a method for achieving THz ultra-broadband coherent absorption using the anti-reflection theory of metamaterials. The metamaterial absorber consists of a periodic array of electric ring resonators with a multilayered structure which form the desired refractive index dispersion and provide continuous anti-reflection over a wide frequency range. The destructive interference mechanism and resonance absorption of the absorber are determined by simulation analysis and numerical simulation. Simulation results show that the absorption bandwidth is almost 8.02 THz (absorption rate >90%) over the entire terahertz band (0.1 THz-10 THz). This design provides an effective and viable method for constructing broadband absorbers for stealth technology and the construction of enhanced transmittance devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9nr10668eDOI Listing
May 2020

The Cerebellum Posterior Lobe Associates with the Exophthalmos of Primary Hyperthyroidism: A Resting-State fMRI Study.

Int J Endocrinol 2019 28;2019:8135671. Epub 2019 Nov 28.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.

Background: Exophthalmos occurs in patients with primary hyperthyroidism. There were few studies about the changes of brain functional networks of patients with exophthalmos of primary hyperthyroidism (EOPH). However, differences in spontaneous brain activity in patients with EOPH remain unclear.

Objective: This study explored alterations in the brain functional networks of patients with EOPH using a voxel-wise degree centrality (DC) method.

Methods: A total of 20 patients with EOPH (8 men and 12 women) were enrolled. In addition, 20 patients with primary hyperthyroidism without exophthalmos, matched in age, sex, and education status, were enrolled as a control group. The Hospital Anxiety and Depression Scale was used to assess the anxiety and depression status of participants. All participants were examined using resting-state functional MRI. Changes in spontaneous brain activity were investigated using the DC method. To distinguish between the DC values of the patients with EOPH and those of the control group, we analyzed the receiver operating characteristic (ROC) curve. The interrelationships between the DC values and clinical variables in the patients with EOPH were evaluated using Pearson's correlation coefficient.

Results: Patients with EOPH exhibited notably lower DC values in the cerebellum posterior lobe than the control group. In addition, there were negative correlations between the anxiety scores (AS) and the depression scores (DS) and DC values of the cerebellum posterior lobe. The ROC curve analysis of the cerebellum posterior lobe demonstrated that the area under the curve method had a high diagnostic accuracy.

Conclusions: Our study was the first, to our knowledge, to explore changes in the brains of patients with EOPH using the DC method. The DC value was significantly different in the cerebellum posterior lobe in patients with EOPH, indicating that the cerebellum posterior lobe is associated with EOPH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2019/8135671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914989PMC
November 2019

[Effects of endurance exercise on synaptic plasticity in cerebral cortex of aged rats and related regulatory mechanism].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2019 Jul;35(4):339-345

Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, Hunan Normal University, Changsha 410012, China.

Objective: To understand and analyze the rules of endurance exercise on the cerebral cortex adaptive mechanism in aged rats.

Methods: In this study, 3-month-old (n=20), 13-month-old (n=24) and 23-month-old (n=24) specific-pathogen free (SPF) male Sprague-Dawley Rat (SD) rats were divided into young (Y-SED), middle-aged (M-SED) and old-aged (O-SED) sedentary control group, and the corresponding Y-EX, M-EX and O-EX in the endurance exercise runner group. The 10-weeks of regular moderate-intensity aerobic exercise intervention were carried out in the endurance exercise runner group. The exercise mode is treadmill exercise (slope 0), and the exercise intensity gradually increases from 60%~65% of the maximum oxygen consumption (V·O) to 70%~75%, and the exercise time is 10 weeks. Hematoxylin and eosin (HE) staining was used to detect age-related morphological changes. The expressions of superoxide dismutase(SOD) and brain-derived neurotrophic factor (BDNF) and the expressions of synapsin 1 (SYN1) and Ca/calmodulin- dependent protein kinases IIα (CaMK IIα) / AMP-activated protein kinase α1(AMPKα1) / mammalian target of rapamycin (mTOR) pathway -related genes were detected.

Results: The cerebral cortex structure of the rats in each group showed age-related aging changes, the expression of SOD in the cortex showed a gradual decline, the expression of BDNF showed an age-increasing trend, and the expression levels of SYN1 and CaMK IIα were increased with age. The changes in AMPKα1 and SirT2 and IP3R, AKT1 and mTOR mRNA levels were increased slightly in middle-aged rats and decreased in aged rats. Compared with the rats in each sedentary control group, the nucleus of the cerebral cortex was tightly arranged and the number of nuclei observed under the microscope was increased significantly in each exercise group. Exercise promoted the expressions of SOD, BDNF and synaptophysin SYN1 in the cortex of rats, and the expression levels of SOD and BDNF in aged rats were up-regulated significantly (P< 0.01). The expression level of SYN1 in rats was up-regulated significantly (P<0.05) in the young and aged rats. The expression of CaMK IIα in the cortex of middle-aged and aged rats was up-regulated (P<0.01), while the expression level of CaMK IIα in young rats was down-regulated (P<0.01). Exercise could up-regulate the expression level of AMPKα1 in the cortex of young rats (P< 0.05), but not in middle-aged and old-age rats. Exercise could up-regulate the expression of SirT2 in the cortex of rats in all age groups (P<0.05). Exercise up-regulated the expression of phosphoinositide 3-kinase (IP3R)/ protein kinase B 1(AKT1) /mTOR in the cortex of rats, among which young IP3R was significantly up-regulated (P<0.01) in the young group, mTOR was significantly up-regulated in young and middle-aged group (P<0.01), and mTOR was also significantly up-regulated in the aged group (P<0.05).

Conclusion: Endurance exercise up-regulates BDNF expression, regulates CaMKIIα signaling, activates AMPK signaling pathway and IP3R / AKT1 / mTOR signaling pathway, and improves synaptic plasticity in the cortex.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12047/j.cjap.5803.2019.072DOI Listing
July 2019

Altered Regional Homogeneity in Patients With Corneal Ulcer: A Resting-State Functional MRI Study.

Front Neurosci 2019 23;13:743. Epub 2019 Jul 23.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Ocular Disease Clinical Research Center, Nanchang, China.

Objective: To investigate the potential regional homogeneity (ReHo) brain activity changes in patients with corneal ulcer (CU) and their possible relationship with clinical symptoms.

Materials And Methods: Forty patients with CU (26 men and 14 women), and 40 healthy controls (HCs) (26 men and 14 women) closely matched in age, sex, and weight underwent resting-state functional MRI scans, respectively. The ReHo method was applied to evaluate synchronous neural activity changes. Receiver operating characteristic curve (ROC curve) was used to show high test-retest stability and high degree of sensitivity and specificity. We utilized the correlation analysis to calculate the relationship between the average ReHo signal values in different brain areas and the clinical symptoms in CU patients.

Results: Compared with the HCs, CU patients had significantly increased ReHo values in right cerebellum posterior lobe, left cerebellum posterior lobe, left inferior temporal gyrus, right lingual gyrus, left middle frontal gyrus, left angular gyrus, left cingulate gyrus, right angular gyrus and bilateral superior frontal gyrus, and decreased ReHo values in right anterior cingulate and left precentral gyrus. ROC curve analysis of each brain regions showed the accuracy of AUC was perfect except the right cerebellum posterior lobe. Nevertheless, there was no clear evidence of prominent relevance between the average ReHo values in brain areas and the clinical symptoms.

Conclusion: Corneal ulcer caused dysfunctional adaption in different brain areas, which including relatively increased values and decreased values. This finding may help us take a further step in exploring the underlying pathologic mechanisms of CU.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2019.00743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664059PMC
July 2019

Altered spontaneous brain activity patterns in patients with corneal ulcer using amplitude of low-frequency fluctuation: An fMRI study.

Exp Ther Med 2019 Jul 7;18(1):125-132. Epub 2019 May 7.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Ocular Disease Clinical Research Center, Nanchang, Jiangxi 330006, P.R. China.

The aim of the present study was to investigate the altered spontaneous brain activity in patients with corneal ulcer (CU) through the amplitude of low-frequency fluctuation (ALFF) technique and the association with their visual performance. A total of 40 patients with CU and 40 healthy controls (HCs) matched for sex, age and educational level were enrolled. Resting-state functional magnetic resonance imaging (rs-fMRI) was performed to examine the probands. Spontaneous cerebral activity variations were investigated using the ALFF technique. The average ALFF values of the CU patients and the HCs were classified by utilizing receiver operating characteristic (ROC) curves. Contrary to HCs, the CU patients had significantly lower ALFF values in the left cerebellar anterior lobe, right middle frontal gyrus and left middle frontal gyrus, but higher ALFF values in the right cerebellar inferior lobe, left cerebellar inferior lobe, left inferior temporal gyrus, right fusiform gyrus, left superior frontal gyrus, right angular gyrus and bilateral superior frontal gyrus. ROC curve analysis of each brain region indicated that the accuracy of ALFF value specificity between the CU and HCs of the area under the curve was perfect. In conclusion, abnormal spontaneous activities were detected in numerous brain regions of CU patients, which may provide useful information for understanding the dysfunction of CU. These activity changes in brain regions may be used as effective clinical indicators for CU.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2019.7550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566102PMC
July 2019

Application of amplitude of low‑frequency fluctuation to altered spontaneous neuronal activity in classical trigeminal neuralgia patients: A resting‑state functional MRI study.

Mol Med Rep 2019 Aug 20;20(2):1707-1715. Epub 2019 Jun 20.

Department of Ophthalmology and Radiology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute, Nanchang, Jiangxi 330006, P.R. China.

Recent studies have reported structural and functional abnormalities in multiple brain regions of classical trigeminal neuralgia (CTN) patients. Differences in spontaneous neuronal activity between CTN patients and healthy subjects, however, remain unknown. The aim of the present study was to investigate alterations in brain activity by application of amplitude of low frequency fluctuation (ALFF), thus analyzing the correlation between durations of spontaneous pain intensity and ALFF values in CTN patients. A total of 28 CTN patients (male, n=12; female, n=16) and 28 healthy controls (HCs; male, n=12; female, n=16) matched for age and sex were enrolled. All subjects underwent resting‑state functional magnetic resonance imaging and changes in spontaneous brain activity were investigated using an ALFF method. Receiver operating characteristic (ROC) curve analysis was applied to differentiate ALFF values of CTN patients from HCs. Altered ALFF values and clinical manifestations were evaluated using Pearson's correlation analysis. ALFF values of the bilateral inferior cerebellum, bilateral fusiform gyrus, right precentral gyrus, left inferior temporal gyrus, right superior cerebellum, left inferior occipital gyrus and right superior occipital gyrus were significantly higher in CTN patients when compared to HCs. ROC curve analysis of each brain revealed a near‑perfect AUC accuracy. Pearson's correlation analysis revealed the visual analog scale of the right eye to be positively correlated with both left inferior temporal and occipital gyral findings, while episode duration likewise was positively associated with left inferior temporal gyral findings. CTN patients exhibited abnormal spontaneous activity in multiple brain regions closely related to pain regulation and perception, while VAS and CTN episode duration were positively correlated with ALFF signal values in some brain regions. The present findings provide further insight into the pathological mechanisms underlying CTN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.10404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625376PMC
August 2019

Diagnostic value of CA-153 and CYFRA 21-1 in predicting intraocular metastasis in patients with metastatic lung cancer.

Cancer Med 2020 02 20;9(4):1279-1286. Epub 2019 Jun 20.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Ocular Disease Clinical Research Center, Nanchang, Jiangxi, People's Republic of China.

Lung cancer is prone to metastasis to various organs. Although intraocular metastasis (IOM) occurs at a later stage than metastasis to other organs, it often adversely affects the quality of life and suggests a poor prognosis. In this study, we selected 1608 patients with lung cancer who had metastasis to at least one site and explored clinical differences between those with IOM and non-IOM (NIOM). An independent t test and chi-squared test were used to analyze the clinical features of the patients. The statistically significant parameters were analyzed by binary logistic regression to determine the risk factors for IOM. A receiver operating characteristic curve was constructed to assess their diagnostic value in IOM. The results showed that no significant differences were noted in age, gender, and pathological type between the IOM and NIOM groups. However, the IOM group had higher levels of alpha-fetoprotein, carcinoembryonic antigen, cancer antigen (CA)-125, CA-153, cytokeratin fragment 19 (CYFRA 21-1), and total prostate-specific antigen, compared with the NIOM group. Binary logistic regression indicated that CA-153 and CYFRA 21-1 were risk factors for IOM in patients with MLC (P < 0.05). Area under the curve of CA-153, CYFRA 21-1 and their combination were 0.791, 0.860, and 0.872 respectively. The cutoff values for CA-153 and CYFRA 21-1 were 22.2 U/mL and 6.785 ng/mL. In conclusion, both CA-153 and CYFRA 21-1 were independent risk factors for IOM in patients with metastatic lung cancer (MLC), whereas the combination of CA-153 and CYFRA 21-1 assessment yields the most value in the detection of IOM in patients with MLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cam4.2354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013068PMC
February 2020

Altered brain network centrality in patients with trigeminal neuralgia: a resting-state fMRI study.

Acta Radiol 2020 Jan 14;61(1):67-75. Epub 2019 May 14.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, PR China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0284185119847678DOI Listing
January 2020

Altered brain activity in patients with strabismus and amblyopia detected by analysis of regional homogeneity: A resting‑state functional magnetic resonance imaging study.

Mol Med Rep 2019 Jun 10;19(6):4832-4840. Epub 2019 Apr 10.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Ocular Disease Clinical Research Center, Nanchang, Jiangxi 330006, P.R. China.

Previous studies have demonstrated that strabismus or amblyopia can result in marked brain function and anatomical alterations. However, differences in spontaneous brain activity in strabismus and amblyopia (SA) patients as compared with control individuals remain unclear. The present study aimed to analyze the potential brain activity changes in SA patients and their association with behavioral performance. In total, 16 patients with SA (10 women and 6 men) and 16 healthy controls (HCs; 6 men and 10 women) with matched age and sex were recruited. All subjects were examined with resting‑state functional magnetic resonance imaging (rs‑fMRI), and changes in the spontaneous brain activity of SA patients were evaluated by the regional homogeneity (ReHo) method. The diagnostic ability of the ReHo method was assessed using receiver operating characteristic (ROC) curve analysis. In addition, the association between the mean ReHo value in different brain regions and the behavioral performance was explored by correlation analysis. It was observed that the ReHo value was significantly increased in SA patients compared with HCs in the following brain regions: left lingual gyrus, right middle occipital gyrus/precuneus, bilateral anterior cingulate, left middle occipital gyrus and bilateral precentral gyrus. By contrast, the ReHo value of the left inferior frontal gyrus was significantly lower than that in HCs. ROC curve analysis indicated that the ReHo method has certain credibility for the diagnosis of SA patients. In addition, no similar changes were detected in other brain regions. These results revealed abnormal spontaneous brain activity in certain parts of the brain in adult patients with SA, which suggests the involvement of the neuropathological or compensatory mechanism in these patients, and may be beneficial for clinical treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.10147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522834PMC
June 2019

Altered Spontaneous Brain Activity in Patients with Classical Trigeminal Neuralgia Using Regional Homogeneity: A Resting-State Functional MRI Study.

Pain Pract 2019 04 25;19(4):397-406. Epub 2019 Jan 25.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Ocular Disease Clinical Research Center, Nanchang, Jiangxi, China.

Objective: Neuroimaging studies have shown that patients with pain-related conditions have altered neuronal activity and structural functions. The purpose of this study was to investigate whether patients with classical trigeminal neuralgia (CTN) exhibit changes in corresponding neuronal activity via analysis of neuronal activity regional homogeneity (ReHo).

Methods: A total of 28 patients presenting with sore eyes (12 men and 16 women) were matched with 28 healthy controls (12 men and 16 women). All participants underwent functional magnetic resonance imaging (fMRI). This ReHo method was used to assess the consistency of changes in neural activity in various brain regions. The receiver operating characteristic (ROC) curve was applied to differentiate ReHo values of patients with CTN from ReHo values of healthy controls. Pearson's correlation analysis was applied to evaluate the correlation between ReHo values of different brain regions of patients with CTN and clinical manifestations.

Results: Compared with healthy controls, patients with CTN were found to have increased ReHo values in the inferior cerebellum bilaterally, right inferior temporal gyrus, right middle occipital gyrus, right fusiform gyrus, right superior frontal gyrus, and right precentral gyrus. ROC curve analysis of each brain region revealed near-perfect accuracy regarding the area under the curve. However, no correlation between ReHo values and clinical manifestations in patients with CTN was found.

Conclusions: CTN is associated with altered neuronal networks in different areas of the brain. ReHo values all possess different degrees of change, implying that CTN has a certain impact on cerebral function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/papr.12753DOI Listing
April 2019

Altered spontaneous brain activity patterns in strabismus with amblyopia patients using amplitude of low-frequency fluctuation: a resting-state fMRI study.

Neuropsychiatr Dis Treat 2018 17;14:2351-2359. Epub 2018 Sep 17.

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute, Nanchang 330006 Jiangxi, China,

Objective: Previous studies have demonstrated that strabismus or amblyopia can result in markedly brain function and anatomical alterations. However, the differences in spontaneous brain activities of strabismus with amblyopia (SA) patients still remain unclear. This current study intended to use the amplitude of low-frequency fluctuation (ALFF) technique to investigate the intrinsic brain activity changes in SA subjects.

Patients And Methods: A total of 16 patients with SA (6 males and 10 females) and 16 healthy controls (HCs; 6 males and 10 females) similarly matched in age, gender, and education status were recruited and examined with the resting-state functional MRI. The spontaneous brain activity changes were investigated using the ALFF technique. The receiver operating characteristic curve was performed to classify the mean ALFF signal values of the SA patients from HCs. The correlations between the ALFF values of distinct brain regions and the clinical manifestations in SA patients were evaluated in terms of the Pearson's correlation analysis.

Results: Compared with HCs, SA patients had significantly decreased ALFF in the left cerebellum posterior lobe, left middle frontal gyrus, and bilateral thalamus. In contrast, SA patients showed increased ALFF values in the right superior frontal gyrus, right precuneus, left cuneus, and bilateral precentral gyrus. Nonetheless, there was no linear correlation between the mean ALFF values in brain regions and clinical features.

Conclusion: Diverse brain regions including vision-related and motion-related areas exhibited aberrant intrinsic brain activity patterns, which imply the neuropathologic mechanisms of oculomotor disorder and vision deficit in the SA patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/NDT.S171462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157537PMC
September 2018

[The effect of different intensity exercise on skeletal muscle fiber MHC subtype transformation and CaN/NFATc1 signaling pathways].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2017 Apr;33(4):360-364

Physical Education, Hunan Norma University Changsha 410012.

Objective: To study the effect of different intensity exercise on skeletal muscle fiber myosin heavy chain(MHC) subtype transformation and CaN/NFATc1 signaling pathways.

Methods: Twenty-four Male SD rats (2-month old) were randomly divided into normal control group (NC), moderate intensity exercise group (ME, grade 5°, speed 18 m/min), heavy intensity exercise group (HE, grade 10°, 26.8 m/min). The rats in exercise groups were treated with treadmill training for eight weeks. The type I and type Ⅱ muscle fibers were determined by ATPase staining method. MHC subtype was separated by SDS-PAGE. The activity of CaN was determined by colorimetric method. The content of NFATc1 protein in skeletal muscle was detected by immune imprinting technology.

Results: ①Skeletal muscle fiber density changes:the type I and Ⅱ fiber number density of quadriceps in ME group were increased significantly (<0.05), but in HE group, only the type Ⅱ fiber surface density was increased significantly (<0.05). The type I fiber number density of soleus in ME and HE group was increased significantly (<0.05). ②The changes of fibers MHC subtype percentage in skeletal muscle:the percentages of MHC I and type Ⅱa of quadriceps in ME group were increased (<0.05), while the percentage of MHC Ⅱb was decrease (<0.05). The percentage of MHC I in soleus was increased, while the percentages of MHCⅡa and Ⅱb were decreased. ③The activity of CaN and the content of NFATc1 protein in ME group were increased significantly (<0.05).

Conclusions: The heavy and moderate intensity exercise may induce skeletal muscle MHC type transforming from fast to slow. At the same time, the activity of CaN and the expression of NFATc1 protein are increased accompanying the changes of skeletal muscle fibers subtype.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12047/j.cjap.5462.2017.087DOI Listing
April 2017

Anti-inflammatory activity effect of 2-substituted-1,4,5,6-tetrahydrocyclopenta[b]pyrrole on TPA-induced skin inflammation in mice.

Bioorg Med Chem Lett 2016 11 15;26(21):5334-5339. Epub 2016 Sep 15.

School of Pharmacy & State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address:

2-Substituted-1,4,5,6-tetrahydrocyclopenta[b]pyrrole, a key structural moiety exiting in many bioactive molecules, has been shown to have excellent selective activity on COX-2. In the present study, the anti-inflammatory activity and the underlying molecular mechanism of 2-substituted-1,4,5,6-tetrahydrocyclopenta[b]pyrrole on skin inflammation were assessed by 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice. Most of the compounds showed anti-inflammatory activity on TPA-induced skin inflammation. The anti-inflammatory activity of compound 4 showed higher anti-inflammatory activity than celecoxib (3.2-fold). Compound 4 pretreatment resulted in markedly suppression of TPA-induced IL-1β, IL-6, TNF-α, and COX-2, respectively. Furthermore, the mechanical study indicated that the anti-inflammatory activity of compound 4 was associated with its ability to inhibit activation of factor kappa-κB (NF-κB) by blocking IκB kinase (IKK) activities. Accordingly, compound 4 could be used as a potential anti-inflammatory agent for skin inflammation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmcl.2016.09.034DOI Listing
November 2016

[The effects of eccentric exercise on the skeletal muscle apoptosis and proliferation in rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2013 Jan;29(1):86-90

Physical Education Department of Hunan Humanities, Science and Technology University, Loudi 417000, China.

Objective: To reveal the effects of three days' repeated exhausted eccentric exercise on the skeletal muscle apoptosis and proliferation in rats.

Methods: Fifty male SD rats aged at 8 week old were randomly divided into control group (C) and training groups (B1, B2, B3, B4) (n = 10), the training groups ran on a treadmill every day till exhausted. After they had been trained repeatedly for three days, their medial head of triceps brachii muscle cell apoptosis was detected in paraffin section by the TUNEL, expression of proliferating cell nuclear antigen (PCNA) protein was examined by immunohistochemistry.

Results: (1) The apoptosis appeared sequential change, and it was consistent with the exercise-induced skeletal muscle micro-injury (EIMmI). The apoptosis index in the training group after exercise was much greater than that in the control group (P < 0.05), and it reached the peak at 24 h after exercise, then it reduced at 48 h after exercise. (2) The express of PCNA exhibited a sequential change after exercise, the proliferation index in the training group after exercise was greater than that in the control group (P < 0.05), it increased after exercise immediately, but it reduced at 3 h after exercise, then was reached the peak at 24 h after exercise, the proliferation index was moderately correlated with the apoptosis index (P < 0.05).

Conclusion: (1) Cell apoptosis can induce the delayed skeletal muscle damage. (2) Apoptosis may be a start factor of skeletal muscle regeneration.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2013

[Study on Polygonum multi florum Thunb and extracts by DRIFTS and atr-ftir].

Guang Pu Xue Yu Guang Pu Fen Xi 2013 Jan;33(1):89-91

Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes, School of Life Science, Guangzhou University, Guangzhou 510006, China.

The diffuse-reflectance FTIR spectroscopy (DRIFTS) and attenuated total reflection FTIR (ATR-FTIR) were used to study polygonum multi florum Thumb and its extracts. The result shows that when acetone is used as extraction agent, the contents of extracts in polygonum multi florum Thunb's phloem are highest, those in polygonum multi florum Thunb's xylem are the lowest. Compared with DRIFTS and ATR-FTIR, it can be found that there are some differences between polygonum multi florum Thunb and its extracts. There are two gentle absorption peaks at 3 576 and 3 147 cm(-1) respectively for polygonum multi florum Thunb, while there is a strong absorption peak at 3 351 cm(-1) for its extracts, showing that there may be more OH... active ingredients in polygonum multi florum Thunb's extracts. Meanwhile, polygonum multiflorum Thunb has strong absorption peaks at 931, 859, 766 and 709 cm(-1) respectively, while its extracts have no resembling absorption peaks. It also shows that the extracts are active ingredients.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2013

Anti-HBV active constituents from Piper longum.

Bioorg Med Chem Lett 2013 Apr 4;23(7):2123-7. Epub 2013 Feb 4.

State Key Laboratory of Phytochemistry and Plant Resource in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, China.

In the screening search for Hepatitis B virus inhibitory agents from medicinal plants, the ethanol extract of Piper longum Linn. was found to possess superior anti-HBV activity in vitro. Bioassay-guided fractionation coupled with repeated purification resulted in the isolation of four new compounds, involving two new glycosides longumosides A (1) and B (2) and two new amide alkaloids erythro-1-[1-oxo-9(3,4-methylenedioxyphenyl)-8,9-dihydroxy-2E-nonenyl]-piperidine (3), threo-1-[1-oxo-9(3,4-methylenedioxyphenyl)-8,9-dihydroxy-2E-nonenyl]-piperidine (4), as well as two compounds 3β,4α-dihydroxy-2-piperidinone (5), 5,6-dihydro-2(1H)-pyridinone (6) from natural source for the first time. The structures of the four new compounds were determined by extensive analyses of the MS, IR, 1D and 2D NMR data. Besides, the compounds 2-6, together with the known compounds 7-11 obtained previously, were assayed for their anti-HBV activity by using Hep G 2.2.15 cell line in vitro. Results suggested the compound piperine (7) possessed remarkable inhibitory HBV activity, against the secretion of hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) with the Selectivity Index (SI) values of 15.7 and 16.8, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmcl.2013.01.118DOI Listing
April 2013

[Characterization of pesticide residual dynamics by in situ attenuated total reflection FTIR].

Guang Pu Xue Yu Guang Pu Fen Xi 2012 Dec;32(12):3217-9

Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes, School of Life Science, Guangzhou University, Guangzhou 510006, China.

In-situ attenuated total reflection FTIR (in situ ATR-FTIR) was used for the dynamic research on the residual of pesticide. The in-situ characterization of dichlorvos and orthene on the tomatoes' surface shows that the dichlorvos has obvious volatility and its degradation amount is 80% 20 minutes after spraying. Meanwhile, the ATR-FTIR shows that the strong absorption peak of dichlorvos at 1 734 cm(-1) turns to negative peaks and the absorption peaks at 3 073 cm(-1) significantly abate. The absorption peaks at 1 277 cm(-1) become weak and red shift (30 cm(-1)) shows that the dichlorvos may be hydrolyzed to some extent. While the absorption peaks of orthene show no change 120 minute after spraying. It shows that the orthene is relatively stable.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2012

New amide alkaloids from Piper longum.

Fitoterapia 2013 Jan 8;84:222-6. Epub 2012 Dec 8.

Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan University of Nationalities, Kunming 650500, China.

Bioassay-guided phytochemical investigation on Piper longum resulted in the isolation of two new compounds. By analyses of the MS, IR, UV, 1D and 2D NMR data, the two new compounds were identified as 3β, 4α-dihydroxy-1-(3-phenylpropanoyl)-piperidine-2-one (1) and (2E, 4E, 14Z)-6-hydroxyl-N-isobutyleicosa-2,4,14-trienamide (2). In vitro anti-HBV bioassay demonstrated compound 1 possessed remarkable activity suppressing the secretion of HBeAg in Hep G2.2.15 cell line, with an IC(50) value of 0.21mM and SI value of 16.4.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fitote.2012.12.001DOI Listing
January 2013

[Effects of psychic ability and glutamine for different time sleep deprivation].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2011 Aug;27(3):361-2, 371, 383

View Article and Find Full Text PDF

Download full-text PDF

Source
August 2011

[A spectral analysis method for total volatile base nitrogen].

Guang Pu Xue Yu Guang Pu Fen Xi 2009 Aug;29(8):2196-8

Schools of Life Science, Guangzhou University, Guangzhou 510006, China.

A new method for the determination of the total volatile basic nitrogen (TVB-N) by reflectance spectroscopy was developed. The method was based on the reaction of TVB-N with Nessler's reagent treated by molecular sieve and the reflectance-absorption value F(R), which is directly proportional to the amount of NH2 Hg IO in solid phase, was measured by the reflection spectrometer. The fundamental principle, effective factors and experimental conditions of the method were discussed. The special features of this method were its simplicity in operation, relatively high sensitivity of determination and the use of a small amount of reagent. Linear calibration graph was obtained in the range 1-8 microg x mL(-1) with a detection limit of 0.1 microg x mL(-1). The method was applied to monitor the fresh degree of fish and pork. The result discovered that the total volatile basic nitrogen increased rapidly along with the time extension, indicating that the deterioration of fish and pork was an acceleration process, and the deterioration speed of fish was faster than that of pork.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2009

Study of the effects of mild hypothermia on cerebral PO2, PCO2 and pH and body temperature in patients with acute severe head injury.

Chin J Traumatol 2005 Jun;8(3):138-41

Department of Neurosurgery, Shenzhen Longgang Central Hospital, Shenzhen 518116, China.

Objective: To study the effects of mild hypothermia on cerebral oxygen partial pressure, carbon dioxide partial pressure, pH and body temperature (PbrO2, PbrCO2, pHbr and BT) in patients with acute severe head injury.

Methods: Thirty-eight patients with acute severe head injury were treated with mild hypothermia, meantime PbrO2, PbrCO2, pHbr and BT were monitored in order to study the changes of PbrO2, PbrCO2, pHbr and BT.

Results: In patients with acute head injury, mild hypothermia obviously increased PbrO2, decreased PbrCO2 and CO2 accumulation and acidosis in brain tissue. BT was 1-1.5 deg. higher than rectal temperature (RT) after injury. The BT and RT were decreased when the patients were treated with mild hypothermia, but at the same time the difference between BT and RT was increased.

Conclusions: In patients with acute severe head injury the direct monitoring of PbrO2, PbrCO2, pHbr and BT was safe and reliable, and is helpful in estimating prognosis and mild hypothermia therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
June 2005

Role of glucose-6-phosphate dehydrogenase in freezing-induced freezing resistance of Populus suaveolens.

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2005 Feb;31(1):34-40

Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing 100083, China.

To explore the role of glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) in the enhancement of freezing resistance induced by freezing acclimation, G6PDH was purified from the leaves of 8-week-old Populus suaveolens cuttings. The G6PDH activity in the absence or the presence of reduced dithiothreitol (DTT(red)) were determined, and the changes in superoxide dismutase (SOD), peroxides (POD) and cytosolic G6PDH activities, malondial-dehyde (MDA) content as well as freezing resistance (expressed as LT(50)) of P. suaveolens cuttings during freezing acclimation at -20 degrees C were investigated. The results showed that the purified G6PDH was probably located in the cytosol of P. suaveolens. Freezing acclimation increased the activities of SOD, POD and cytosolic G6PDH, and decreased the MDA content and LT(50) of cuttings, while 2 d of de-acclimation at 25 degrees C resulted in a decrease in SOD, POD and cytosolic G6PDH activities, and caused an increase in MDA content and LT(50). The change in cytosolic G6PDH activity was found to be closely correlated to the levels of SOD, POD and MDA, and to the degree of freezing resistance of cuttings during freezing acclimation. It is suggested that the enhancement of freezing resistance of cuttings induced by freezing acclimation is related to the distinct increase in cytosolic G6PDH activity, which may be involved in the activation of SOD and POD, and the induction of freezing resistance of cuttings.
View Article and Find Full Text PDF

Download full-text PDF

Source
February 2005

[Effect of moderate hypothermia on intracranial pressure and brain tissue oxygen tension in head injury].

Di Yi Jun Yi Da Xue Xue Bao 2004 Aug;24(8):963-4

Department of Neurosurgery, Shenzhen Longgang Central Hospital, Shenzhen 518116, China.

Objective: To observe the changes in intracranial pressure (ICP) and brain tissue oxygen tension (PO(2)) and their significance in severe head injury treated by moderate hypothermia (33-35 degrees Celsius).

Methods: Eighteen patients with severe head injury were treated with moderate hypothermia group and probes were deployed in the cerebral cortex for measuring their ICP and PO(2) under continuous monitoring. Another 14 similar patients receiving the same treatment but without hypothermia served as the control group.

Results: In moderate hypothermia group the ICP gradually decreased and PO(2) increased after 24 hours of hypothermia, with obvious difference from the control group (P<0.05).

Conclusion: Moderate hypothermia can obviously reduce ICP and improve brain tissue oxygen metabolism to lower the morbidity and mortality rate of severe head injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2004
-->