Publications by authors named "Wen Wang"

1,719 Publications

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pH and light-responsive polycaprolactone/[email protected] composite films with enhanced antibacterial activity.

J Food Sci 2021 Jul 13. Epub 2021 Jul 13.

Sino-Singapore International Joint Research Institute, Guangzhou, China.

Food packaging materials, especially biodegradable polymer composites incorporated with natural antimicrobial agents with excellent antibacterial activities, are in high demand and attracted immense attention. Herein, a polycaprolactone/[email protected] imidazolate framework-8 (PCL/[email protected]) composite film with enhanced antibacterial activity was developed. Curcumin, a natural photosensitizer, was loaded in the highly porous nanocrystals ZIF-8 to improve its poor water solubility and stability. The integral structure of [email protected] was maintained well in the PCL matrix even at the highest loading of 35% (w/w), and all composite films had good light transmittance at 420-430 nm. The PCL/[email protected] composite films responded to the acidic growth environment of bacteria by releasing zinc ions and curcumin molecules. Furthermore, upon blue light irradiation (420-430 nm, 2.2 mW/cm ), curcumin molecules generated singlet oxygen. With the synergistic effects of zinc ions and singlet oxygen, the composite films exhibited a 99.9% reduction of Escherichia coli and Staphylococcus aureus strains when the amount of [email protected] loading was more than 15% (w/w), as well as a strong anti-adhesion effect on bacteria. Moreover, bacterial resuscitation tests indicated that the composite films exhibited 99.9% reduction in the adhered bacteria population through treatment with photodynamic sterilization. This is the first study presenting that the incorporated curcumin ZIF-8 nanoparticles in the matrix of polymer are pH and light responsive for anti-adhesion of bacteria, which is of great potential application as antibacterial packaging material for the food industry. PRACTICAL APPLICATION: A novel, biodegradable, pH, and light-responsive composite film was developed for antibacterial activity. Natural photosensitizer curcumin was encapsulated in ZIF-8 nanocrystals ([email protected]) as the antimicrobial agent. With the synergistic effects of Zn and singlet oxygen, the composite film exhibited a 99.9% reduction of Escherichia coli and Staphylococcus aureus strains, and a strong anti-adhesion property toward bacteria. This composite film is of great potential application as an antibacterial packaging material that enhances the shelf life of fruits, meat, and so on.
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http://dx.doi.org/10.1111/1750-3841.15839DOI Listing
July 2021

Awareness, attitude and barriers of colorectal cancer screening among high-risk populations in China: a cross-sectional study.

BMJ Open 2021 Jul 12;11(7):e045168. Epub 2021 Jul 12.

Department of International and Humanistic Nursing, School of Nursing, University of South China, Hengyang, Hunan, China

Objective: To assess the awareness, attitude and barriers of colorectal cancer screening among high-risk populations in China.

Design: A cross-sectional study was employed.

Setting: This study was conducted in nine hospitals in Hunan province, China.

Participants: Individuals with a high-risk for colorectal cancer were interviewed using a pretested structured questionnaire.

Primary And Secondary Outcome Measures: Knowledge, attitude towards colorectal cancer screening, sociodemographic factors associated with screening knowledge and behaviour and barriers of colorectal cancer screening.

Results: This study included 684 participants. The mean knowledge score was 11.86/24 (SD 4.84). But over 70% of them held a positive attitude towards screening. Only 13.3% had undergone colorectal cancer screening. Independent factors related to knowledge were education level of college or above, working as a white collar, higher income, having health insurance, having seen a doctor in the past year and with a high perceived risk (p<0.05). Factors independently associated with screening behaviour included personal history of colorectal disease, having seen a doctor in the past year, previous discussion of colorectal cancer screening, high perceived risk and better knowledge (p<0.05). Main reasons for not undergoing screening were no symptoms or discomfort (71.1%), never having thought of the disease or screening (67.4%) and no doctor advised me (29.8%).

Conclusion: In China, the majority of high-risk people had deficient knowledge and had never undergone colorectal cancer screening. But most of them held a positive attitude towards the benefits of colorectal cancer screening. This has promising implications to design targeted educational campaigns and establish screening programmes to improve colorectal cancer awareness and screening participation. Healthcare professionals should advise high-risk individuals to participate in screening and inform them about cancer risk.
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http://dx.doi.org/10.1136/bmjopen-2020-045168DOI Listing
July 2021

Identification and Characterization of Robust Hepatocellular Carcinoma Prognostic Subtypes Based on an Integrative Metabolite-Protein Interaction Network.

Adv Sci (Weinh) 2021 Jul 11:e2100311. Epub 2021 Jul 11.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Metabolite-protein interactions (MPIs) play key roles in cancer metabolism. However, our current knowledge about MPIs in cancers remains limited due to the complexity of cancer cells. Herein, the authors construct an integrative MPI network and propose a MPI network based hepatocellular carcinoma (HCC) subtyping and mechanism exploration workflow. Based on the expressions of hub proteins on the MPI network, two prognosis-distinctive HCC subtypes are identified. Meanwhile, multiple interdependent features of the poor prognostic subtype are observed, including hypoxia, DNA hypermethylation of metabolic pathways, fatty acid accumulation, immune pathway up-regulation, and exhausted T-cell infiltration. Notably, the immune pathway up-regulation is probably induced by accumulated unsaturated fatty acids which are predicted to interact with multiple immune regulators like SRC and TGFB1. Moreover, based on tumor microenvironment compositions, the poor prognostic subtype is further divided into two sub-populations showing remarkable differences in metabolism. The subtyping shows a strong consistency across multiple HCC cohorts including early-stage HCC. Overall, the authors redefine robust HCC prognosis subtypes and identify potential MPIs linking metabolism to immune regulations, thus promoting understanding and clinical applications about HCC metabolism heterogeneity.
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http://dx.doi.org/10.1002/advs.202100311DOI Listing
July 2021

A systematic review and meta-analysis of early diagnosis and treatment of hypertensive stroke under calcium channel blockers.

Ann Palliat Med 2021 Jun;10(6):6715-6725

Department of Neurosurgery, The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.

Background: A systematic evaluation of the therapeutic effects of calcium channel blockers (CCB) on stroke is carried out to provide clinical evidence for their application.

Methods: A search for randomized controlled trials (RCTs) of CCB in the treatment of stroke patients in the electronic databases of PubMed, Embase, Medline, Spring, and Ovid from their establishment to January 31, 2021 was performed, and the collected studies were then screened for the exclusion criteria. The Cochrane Handbook version 5.0.2 system evaluation writing manual was adopted to evaluate the risk of bias for the included literature, and a meta-analysis using Review Manager 5.3 software was applied.

Results: A total of 13 RCTs were included, comprising 1,067 subjects. The meta-analysis showed that the recurrence rate of stroke in patients from the observation group reduced sharply after CCB treatment [mean difference (MD) =0.41, 95% confidence interval (CI): 0.24-0.70, Z=3.31, P=0.0009], the Mini-Mental State Examination (MMSE) score improved markedly (MD =2.82, 95% CI: 1.69-3.95, Z=4.89, P<0.00001), and the Montreal Cognitive Assessment (MoCA) score was obviously increased (MD =6.07, 95% CI: 0.34-11.81, Z=2.08, P=0.04). Moreover, the diastolic blood pressure of the observation group decreased steeply after CCB treatment (MD =-1.11, 95% CI: -2.06-0.15, Z=2.27, and P=0.02). However, the effective rate of clinical treatment did not increase hugely (MD =1.70, 95% CI: 0.50-5.83, Z=0.85, P=0.40), and systolic blood pressure did not drop sharply (MD =-1.24, 95% CI: -2.85-0.37, Z=1.51, P=0.13).

Discussion: CCB treatment of stroke effectively prevented stroke recurrence, and showed faster recovery of cognitive function, and better lowering of blood pressure, all of which makes CCBs suitable for the treatment of stroke.
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http://dx.doi.org/10.21037/apm-21-1272DOI Listing
June 2021

Effectiveness of omalizumab in patients with severe allergic asthma: A retrospective study in China.

Respir Med 2021 Jun 29;186:106522. Epub 2021 Jun 29.

Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: We conducted the first real-world study of treatment with omalizumab, a humanized monoclonal anti-immunoglobulin E antibody, in Chinese patients with severe allergic asthma.

Objective: The primary objective was the steroid-sparing effect of omalizumab after 12 and 16 weeks of treatment. Characteristics of the patient population, treatment patterns, response rate, and other measures of therapeutic effectiveness were also reported.

Methods: This nationwide, retrospective, real-world study was conducted in patients with severe allergic asthma who were treated with omalizumab in China. Data, including demographics, Asthma Control Test (ACT) and laboratory and lung function test results, and omalizumab use information, were extracted from patient records collected as part of a previously conducted real-world survey (Asthma Group of the Respiratory Disease Society of the Chinese Medical Association).

Results: In total, 139 patient records were included; 131 and 118 patients remained on treatment at the ≥12- and ≥16-week time points, respectively. The mean ± standard deviation age and median asthma duration (interquartile range) were 47.4 ± 14.3 and 7 (4, 15) years, respectively; 75.6% of patients had a history of allergic disease. Reductions (versus baseline) in inhaled corticosteroid/long-acting β2 agonists or oral corticosteroids were reported in 61.1% and 63.6% of patients at ≥12 and ≥ 16 weeks, respectively. There were significant improvements in ACT scores (6.08, P < .001) and nitric oxide fraction in exhaled air (-13.0, P = .01) from baseline. Multivariate analysis revealed that age and allergic medical history were predictors of omalizumab treatment response. No serious adverse events were reported.

Conclusion: Real-world omalizumab treatment was efficacious and well-tolerated in Chinese patients with severe allergic asthma.
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http://dx.doi.org/10.1016/j.rmed.2021.106522DOI Listing
June 2021

Differentiating thymoma, thymic carcinoma and lymphoma based on collagen fibre patterns with T2- and diffusion-weighted magnetic resonance imaging.

Eur Radiol 2021 Jul 2. Epub 2021 Jul 2.

Department of Radiology, Tangdu Hospital, Air Force Medical University (Fourth Military Medical University), Xi'an, 710038, Shaanxi, People's Republic of China.

Objectives: The amount and distribution of intratumoural collagen fibre vary among different thymic tumours, which can be clearly detected with T2- and diffusion-weighted MR images. To explore the incidences of collagen fibre patterns (CFPs) among thymomas, thymic carcinomas and lymphomas on imaging, and to evaluate the efficacy and reproducibility of CFPs in differential diagnosis of thymic tumours.

Materials And Methods: Three hundred and ninety-eight patients with pathologically diagnosed thymoma, thymic carcinoma and lymphoma who underwent T2- and diffusion-weighted MR imaging were retrospectively enrolled. CFPs were classified into four categories: septum sign, patchy pattern, mixed pattern and no septum sign. The incidences of CFPs were compared among different thymic tumours, and the efficacy and reproducibility in differentiating the defined tumour types were analysed.

Results: There were significant differences in CFPs among thymomas, thymic squamous cell carcinomas (TSCCs), other thymic carcinomas and neuroendocrine tumours (OTC&NTs) and thymic lymphomas. Septum signs were found in 209 (86%) thymomas, which differed between thymomas and any other thymic neoplasms (all p < 0.005). The patchy, mixed patterns and no septum sign were mainly seen in TSCCs (80.3%), OTC&NTs (78.9%) and thymic lymphomas (56.9%), respectively. The consistency of different CFP evaluation between two readers was either good or excellent. CFPs achieved high efficacy in identifying the thymic tumours.

Conclusion: The CFPs based on T2- and diffusion-weighted MR imaging were of great value in the differential diagnosis of thymic tumours.

Key Points: • Significant differences are found in intratumoural collagen fibre patterns among thymomas, thymic squamous cell carcinomas, other thymic carcinomas and neuroendocrine tumours and thymic lymphomas. • The septum sign, patchy pattern, mixed pattern and no septum sign are mainly seen in thymomas (86%), thymic squamous cell carcinomas (80.3%), other thymic carcinomas and neuroendocrine tumours (79%) and thymic lymphomas (57%), respectively. • The collagen fibre patterns have high efficacy and reproducibility in differentiating thymomas, thymic squamous cell carcinomas and thymic lymphomas.
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http://dx.doi.org/10.1007/s00330-021-08143-xDOI Listing
July 2021

Developing a research database of primary aldosteronism: rationale and baseline characteristics.

BMC Endocr Disord 2021 Jun 29;21(1):137. Epub 2021 Jun 29.

Department of Endocrinology and Metabolism, Adrenal Center, West China Hospital, Sichuan University, Chengdu, 610041, China.

Background: Management of primary aldosteronism (PA) has become a research hotspot in the field of endocrinology. To obtain reliable research evidence, it is necessary to establish a high-quality PA research database.

Methods: The establishment of PA research database involved two steps. Firstly, patients with confirmation of PA diagnosis between 1 Jan 2009 to 31 Aug 2019 at West China Hospital were identified and data were extracted. Secondly, patients with confirmatory testing for PA will be enrolled into a prospective cohort. Data will be prospectively collected based on the case report forms since 1 Sep 2019. We evaluated the quality of research database through assessment of quality of key variables.

Results: Totally, 862 patients diagnosed as PA were identified, of which 507 patients who had positive confirmatory testing for PA were included into the retrospective database. Among 862 patients diagnosed as PA, the mean systolic blood pressure (SBP) was 156.1 (21.7) mmHg, mean diastolic blood pressure (DBP) was 97.2 (14.5) mmHg. Among included patients, the mean serum potassium level was 2.85 (IQR, (2.47-3.36) mmol/L, and the mean plasma aldosterone concentration (PAC) was 28.1 (IQR, 20.0-40.4) ng/dL. The characteristics of patients with positive confirmatory testing for PA were similar. Validation of data extracting and linking showed the accuracy were 100%. Evaluation of missing data showed that the completeness of BMI (95.9%), SBP (99.4%) and DBP (99.4%) were high.

Conclusion: Through integrating retrospective and prospective cohort of PA, a research database of PA with high quality and comprehensive data can be established. We anticipate that the research database will provide a high level of feasibility for management of PA in China.
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http://dx.doi.org/10.1186/s12902-021-00794-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244177PMC
June 2021

Puerarin Ameliorates Caerulein-Induced Chronic Pancreatitis Inhibition of MAPK Signaling Pathway.

Front Pharmacol 2021 2;12:686992. Epub 2021 Jun 2.

Department of Digestive Diseases, 900TH Hospital of Joint Logistics Support Force, Fuzhou General Clinical Medical College of Fujian Medical University, Oriental Hospital Affiliated to Xiamen University, Fuzhou, China.

Pancreatic fibrosis is one of the most important pathological features of chronic pancreatitis (CP), and pancreatic stellate cells (PSCs) are considered to be the key cells. Puerarin is the most important flavonoid active component in Chinese herb , and it exhibited anti-fibrotic effect in various fibrous diseases recently. However, the impact and molecular mechanism of puerarin on CP and pancreatic fibrosis remain unknown. This study systematically investigated the effect of puerarin on CP and pancreatic fibrosis and . H&E staining, Sirius Red staining, qRT-PCR and Western blotting analysis of fibrosis and inflammation related genes of pancreatic tissues showed that puerarin notably ameliorated pancreatic atrophy, inflammation and fibrosis in a model of caerulein-induced murine CP. Western blotting analysis of pancreatic tissues showed the phosphorylation level of MAPK family proteins (JNK1/2, ERK1/2 and p38 MAPK) significantly increased after modeling of cerulein, while puerarin could inhibit their phosphorylation levels to a certain extent. We found that puerarin exerted a marked inhibition on the proliferation, migration and activation of PSCs, determined by CCK-8 assay, transwell migration assay, scratch wound-healing assay and expression levels of α-SMA, Fibronectin, Col1α1 and GFAP. Western blotting result demonstrated that puerarin markedly inhibited the phosphorylation of MAPK family proteins (JNK1/2, ERK1/2 and p38 MAPK) of PSCs in a dose-dependent manner whether or not stimulated by platelet-activating factor. In conclusion, the present study showed that puerarin could be a potential therapeutic candidate in the treatment of CP, and the MAPK pathway might be its important target.
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http://dx.doi.org/10.3389/fphar.2021.686992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207514PMC
June 2021

Arhgef2 regulates neural differentiation in the cerebral cortex through mRNA mA-methylation of Npdc1 and Cend1.

iScience 2021 Jun 24;24(6):102645. Epub 2021 May 24.

Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China.

-methyladenosine (mA) is emerging as a vital factor regulating neural differentiation. Here, we report that deficiency of , a novel cause of a neurodevelopmental disorder we identified recently, impairs neurogenesis, neurite outgrowth, and synaptic formation by regulating mA methylation. knockout decreases expression of and total mA level significantly in the cerebral cortex. mA sequencing reveals that loss of reduces mA methylation of 1,622 mRNAs, including and which are both strongly associated with cell cycle exit and terminal neural differentiation. deficiency decreases mA methylations of the and mRNAs via down-regulation of Mettl14, and thereby inhibits the translation of and nuclear export of mRNAs. Overexpression of Mettl14, Npdc1, and Cend1 rescue the abnormal phenotypes in knockout mice, respectively. Our study provides a critical insight into a mechanism by which defective mediates mA-tagged target mRNAs to impair neural differentiation.
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http://dx.doi.org/10.1016/j.isci.2021.102645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185223PMC
June 2021

Printable Zinc-Ion Hybrid Micro-Capacitors for Flexible Self-Powered Integrated Units.

Nanomicro Lett 2020 Nov 5;13(1):19. Epub 2020 Nov 5.

State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.

Wearable self-powered systems integrated with energy conversion and storage devices such as solar-charging power units arouse widespread concerns in scientific and industrial realms. However, their applications are hampered by the restrictions of unbefitting size matching between integrated modules, limited tolerance to the variation of input current, reliability, and safety issues. Herein, flexible solar-charging self-powered units based on printed Zn-ion hybrid micro-capacitor as the energy storage module is developed. Unique 3D micro-/nano-architecture of the biomass kelp-carbon combined with multivalent ion (Zn) storage endows the aqueous Zn-ion hybrid capacitor with high specific capacity (196.7 mAh g at 0.1 A g). By employing an in-plane asymmetric printing technique, the fabricated quasi-solid-state Zn-ion hybrid micro-capacitors exhibit high rate, long life and energy density up to 8.2 μWh cm. After integrating the micro-capacitor with organic solar cells, the derived self-powered system presents outstanding energy conversion/storage efficiency (η = 17.8%), solar-charging cyclic stability (95% after 100 cycles), wide current tolerance, and good mechanical flexibility. Such portable, wearable, and green integrated units offer new insights into design of advanced self-powered systems toward the goal of developing highly safe, economic, stable, and long-life smart wearable electronics.
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http://dx.doi.org/10.1007/s40820-020-00546-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187672PMC
November 2020

Integrative analysis of TP73 profile prognostic significance in WHO grade II/III glioma.

Cancer Med 2021 Jul 13;10(13):4644-4657. Epub 2021 Jun 13.

Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Due to the extremely intrinsic heterogeneity among glioma patients, the outcomes of these patients are tremendously different. Therefore, the exploitation of novel biomarker classification of glioma is vitally important for deep insight into the essence and predicting the prognosis of glioma. We aim to analyze the correlation between TP73 mRNA expression, DNA methylated alteration and the prognosis of WHO grade II/III glioma, utilizing bioinformatics to evaluate its significance as a risk-factor in predicting the prognosis of these glioma patients. The analysis found that TP73 expression was positively correlated with the grade of glioma, and showed a strong correlation with glioma molecular classification, which revealed significantly higher TP73 expression in IDH-wildtype than in IDH-mutant subtype of WHO grade II/III glioma. Cox regression analysis indicated that high expression of TP73 shared an independent high-risk factor impacting the prognosis of WHO grade II/III glioma. We discovered 8 DNA promoter methylation sites with prognostic significance, which were negatively associated with TP73 expression, and positively associated with beneficial overall survival (OS) and progression-free survival (PFS). Integrating with four independent glioma datasets, subsequent Meta-analysis verified that low expression of TP73 was closely related to favorable OS, especially in IDH-mutant subtype. Moreover, we found that 1p/19q /TP73 subgroup shared the most favorable OS, 1p/19q /TP73 subgroup suffered the worst OS. Meanwhile, the enrichment analysis of TP73-related differential mRNAs demonstrated that TP73 aberration in WHO grade II/III glioma might be closely related to cell cycle and P53 signaling pathways. Finally, TP73 expression of 53 glioma specimens was measured by qRT-PCR, which was consistent with the previous analytical result, and TP73 high-expression subgroup suffered worse PFS than TP73 low-expression subgroup. In summary, our funding supports that TP73 gene can perform as a reliable biomarker to evaluate the survival outcome of patients diagnosed with WHO grade II/III glioma.
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http://dx.doi.org/10.1002/cam4.4016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267133PMC
July 2021

Bio-electrochemically extracted nitrogen from residual resources for microbial protein production.

Bioresour Technol 2021 Jun 8;337:125353. Epub 2021 Jun 8.

Biomass Energy and Environmental Engineering Research Center, Beijing University of Chemical Technology, Beijing 100029, China; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:

Upcycling of nutrients from residual resources for producing microbial protein (MP) is an attractive method to valorize residues. In this study, we investigated bio-electrochemical methods to recover ammonia-N, for further production of MP. Reject water and digestate were used for ammonia-N recovery in microbial fuel cell (MFC) system. In one-stage process, ammonia-N recovery was 32 - 42% with 57 - 154 kJ/m waste stream of electricity generation. For further enhancing recovery efficiency, a two-stage process was developed, achieving efficiency of 53 - 61%. Subsequently, MP was grown with the extracted ammonia-N, and amino acid concentration was 421 and 272 mg/L under 25 °C and 35 °C, respectively. Similar essential amino acid content of MP (especially under 25 °C) with the one from fish demonstrated the attractiveness of upcycling residues to proteins. Based on simplified economic evaluation, the produced energy performed the potential to catch 1.63 - 6.54 €/m waste stream.
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http://dx.doi.org/10.1016/j.biortech.2021.125353DOI Listing
June 2021

Giraffa camelopardalis.

Trends Genet 2021 Jun 7. Epub 2021 Jun 7.

School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China. Electronic address:

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http://dx.doi.org/10.1016/j.tig.2021.05.004DOI Listing
June 2021

YB1 regulates miR-205/200b-ZEB1 axis by inhibiting microRNA maturation in hepatocellular carcinoma.

Cancer Commun (Lond) 2021 Jul 10;41(7):576-595. Epub 2021 Jun 10.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, P. R. China.

Background: Y-box binding protein 1 (YB1 or YBX1) plays a critical role in tumorigenesis and cancer progression. However, whether YB1 affects malignant transformation by modulating non-coding RNAs remains largely unknown. This study aimed to investigate the relationship between YB1 and microRNAs and reveal the underlying mechanism by which YB1 impacts on tumor malignancy via miRNAs-mediated regulatory network.

Methods: The biological functions of YB1 in hepatocellular carcinoma (HCC) cells were investigated by cell proliferation, wound healing, and transwell invasion assays. The miRNAs dysregulated by YB1 were screened by microarray analysis in HCC cell lines. The regulation of YB1 on miR-205 and miR-200b was determined by quantitative real-time PCR, dual-luciferase reporter assay, RNA immunoprecipitation, and pull-down assay. The relationships of YB1, DGCR8, Dicer, TUT4, and TUT1 were identified by pull-down and coimmunoprecipitation experiments. The cellular co-localization of YB1, DGCR8, and Dicer were detected by immunofluorescent staining. The in vivo effect of YB1 on tumor metastasis was determined by injecting MHCC97H cells transduced with YB1 shRNA or shControl via the tail vein in nude BALB/c mice. The expression levels of epithelial to mesenchymal transition markers were detected by immunoblotting and immunohistochemistry assays.

Results: YB1 promoted HCC cell migration and tumor metastasis by regulating miR-205/200b-ZEB1 axis partially in a Snail-independent manner. YB1 suppressed miR-205 and miR-200b maturation by interacting with the microprocessors DGCR8 and Dicer as well as TUT4 and TUT1 via the conserved cold shock domain. Subsequently, the downregulation of miR-205 and miR-200b enhanced ZEB1 expression, thus leading to increased cell migration and invasion. Furthermore, statistical analyses on gene expression data from HCC and normal liver tissues showed that YB1 expression was positively associated with ZEB1 expression and remarkably correlated with clinical prognosis.

Conclusion: This study reveals a previously undescribed mechanism by which YB1 promotes cancer progression by regulating the miR-205/200b-ZEB1 axis in HCC cells. Furthermore, these results highlight that YB1 may play biological functions via miRNAs-mediated gene regulation, and it can serve as a potential therapeutic target in human cancers.
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http://dx.doi.org/10.1002/cac2.12164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286141PMC
July 2021

[Study on Adverse Events of Infant Incubator Products].

Zhongguo Yi Liao Qi Xie Za Zhi 2021 Jun;45(3):335-339

Center for Medical Device Adverse Events Monitoring of Zhejiang, Hangzhou, 311121.

Objective: To investigate the risk of adverse events in the clinical use of infant incubators of three kinds of national medical devices in Zhejiang Province.

Methods: Semi-quantitative matrix analysis was used to analyze the risk of adverse events related to incubator products in Zhejiang province from August 2018 to August 2019.

Results: Through the risk analysis of 213 cases of adverse events of infant incubator product, the risk point and degree of the product in practical clinical application were evaluated.

Conclusions: Through the evaluation results of the production enterprises, medical institutions put forward relevant recommendations to reduce the risk of product use to prevent the recurrence of serious adverse events in the use of the product and spread.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2021.03.022DOI Listing
June 2021

Methane production from acetate, formate and H/CO under high ammonia level: Modified ADM1 simulation and microbial characterization.

Sci Total Environ 2021 Aug 7;783:147581. Epub 2021 May 7.

College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Biomass Energy and Environmental Engineering Research Center, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:

This study evaluated the methanogenic performance of typical substrates (acetate, formate, H/CO, and glucose) under low and high ammonia levels and the Anaerobic Digestion Model No.1 (ADM1) was extended and modified for better simulation and understanding of the process. Formate-utilizing and hydrogen-utilizing methanogenesis showed stronger ammonia resistance than acetate-utilizing methanogenesis (13-23% vs. 34% decrease in methane production (MP)). Model extension, based on foundational experiments fed with three typical precursors (R > 0.92), was then validated with glucose degradation experiments, and satisfactory predictions of MP and total volatile fatty acids were obtained (R > 0.91). Based on the modified ADM1, the carbon fluxes of glucose degradation were determined, and formate-utilizing methanogenesis showed its importance with a 28-34% contribution of the total methanation, becoming the dominant pathway under high ammonia level. Formate-utilizing methanogenesis also had a thermodynamic advantage among the three pathways. 16S rRNA sequencing suggested a homology between the hydrogen-utilizing and formate-utilizing methanogens. Methanobacterium and Methanobrevibacter were found to be key methanogens, and their enrichment under high ammonia level confirmed the stronger ammonia tolerance of formate-utilizing and hydrogen-utilizing methanogenesis. The microbial characterization and modified ADM1 simulations supported each other.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147581DOI Listing
August 2021

A core-shell structured COVID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity.

Signal Transduct Target Ther 2021 05 31;6(1):213. Epub 2021 May 31.

NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Although inoculation of COVID-19 vaccines has rolled out globally, there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries. Here, we report on the development of a highly efficacious mRNA vaccine, SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex (LPP) nanoparticles. SW0123 is easy to produce using a large-scale microfluidics-based apparatus. The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability, and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration. Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123, represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2, but also a panel of variants including D614G and N501Y variants. In addition, SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge. Taken together, SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans.
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http://dx.doi.org/10.1038/s41392-021-00634-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165147PMC
May 2021

Viral Gene Therapy for Glioblastoma Multiforme: A Promising Hope for the Current Dilemma.

Front Oncol 2021 13;11:678226. Epub 2021 May 13.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Glioblastoma multiforme (GBM), as one of the most common malignant brain tumors, was limited in its treatment effectiveness with current options. Its invasive and infiltrative features led to tumor recurrence and poor prognosis. Effective treatment and survival improvement have always been a challenge. With the exploration of genetic mutations and molecular pathways in neuro-oncology, gene therapy is becoming a promising therapeutic approach. Therapeutic genes are delivered into target cells with viral vectors to act specific antitumor effects, which can be used in gene delivery, play an oncolysis effect, and induce host immune response. The application of engineering technology makes the virus vector used in genetics a more prospective future. Recent advances in viral gene therapy offer hope for treating brain tumors. In this review, we discuss the types and designs of viruses as well as their study progress and potential applications in the treatment of GBM. Although still under research, viral gene therapy is promising to be a new therapeutic approach for GBM treatment in the future.
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http://dx.doi.org/10.3389/fonc.2021.678226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155537PMC
May 2021

An Improvement of Survival Stratification in Glioblastoma Patients via Combining Subregional Radiomics Signatures.

Front Neurosci 2021 13;15:683452. Epub 2021 May 13.

Institute of Medical Research, Northwestern Polytechnical University, Xi'an, China.

Purpose: To investigate whether combining multiple radiomics signatures derived from the subregions of glioblastoma (GBM) can improve survival prediction of patients with GBM.

Methods: In total, 129 patients were included in this study and split into training ( = 99) and test ( = 30) cohorts. Radiomics features were extracted from each tumor region then radiomics scores were obtained separately using least absolute shrinkage and selection operator (LASSO) COX regression. A clinical nomogram was also constructed using various clinical risk factors. Radiomics nomograms were constructed by combing a single radiomics signature from the whole tumor region with clinical risk factors or combining three radiomics signatures from three tumor subregions with clinical risk factors. The performance of these models was assessed by the discrimination, calibration and clinical usefulness metrics, and was compared with that of the clinical nomogram.

Results: Incorporating the three radiomics signatures, i.e., Radscores for ET, NET, and ED, into the radiomics-based nomogram improved the performance in estimating survival (C-index: training/test cohort: 0.717/0.655) compared with that of the clinical nomogram (C-index: training/test cohort: 0.633/0.560) and that of the radiomics nomogram based on single region radiomics signatures (C-index: training/test cohort: 0.656/0.535).

Conclusion: The multiregional radiomics nomogram exhibited a favorable survival stratification accuracy.
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http://dx.doi.org/10.3389/fnins.2021.683452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161502PMC
May 2021

Correction to: Demographic Characteristics and Clinical Features of Fibromyalgia in China: A Cross-Sectional Study.

Rheumatol Ther 2021 Jun;8(2):833

Rheumatology Department, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, No. 5 Beixiange Street, Beijing, 100053, Xicheng District, China.

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http://dx.doi.org/10.1007/s40744-021-00320-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217408PMC
June 2021

A preliminary study on the characteristics of Th1/Th2 immune response in cerebrospinal fluid of AIDS patients with cryptococcal meningitis.

BMC Infect Dis 2021 May 29;21(1):500. Epub 2021 May 29.

Center for Infectious Diseases, Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China.

Background: Cryptococcal Meningitis (CM) is a common opportunistic infection in the late stage of acquired immunodeficiency syndrome (AIDS). Despite the wide use of effective antiretroviral and antifungal therapy in AIDS patients, CM is still a major morbidity and mortality cause. Understanding the immune response in cryptococcal infection may help to improve the treatment strategies.

Methods: We established a prospective cohort of twelve AIDS patients with CM (HIV + CM+) admitted to the hospital from 2019 to 2020. All patients were examined at the baseline, 2 weeks, and 4 weeks thereafter. The level of 19 cytokines in cerebrospinal fluid (CSF) were recorded to analyze the characteristics and dynamic changes of Th1/Th2 immune response. Meanwhile, six AIDS patients without CM (HIV + CM-) and seventeen healthy subjects (HIV-CM-) were included as control groups for CSF assessment.

Results: The HIV+ CM+ group had higher CSF IFN-γ, TNF-α, IL-6, IL-7, IL-8, IL-10, IL-12 (P40), IL-15, IL-18, CCL2 levels but lower IL-4 when compared with the HIV-CM- group at baseline. And they also had a higher level of IL-12 (P40) and IL-17A compared with HIV + CM- patients. Except one patient dropped out of the study, eleven HIV + CM+ patients received induction antifungal therapy and regular CSF testing, and the mortality rate was 9.1% (1/11) and 18.2% (2/11) respectively at week 2 and week 4. Compared with baseline CSF cytokines, IL-2, IL-13, IL-17A, and VEGF-A decreased in week 2, and the VEGF-A levels further decreased in week 4. But there was no difference in the levels of all cytokines between survivors and the dead.

Conclusion: No evidence of Th1/Th2 imbalance was found in AIDS patients with CM. However, the CSF cytokine network may provide new clues for the treatment of AIDS patients with CM.

Trial Registration: This trial was prospectively registered in 2019.7.16. The registered number is ChiCTR1900024565 .
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http://dx.doi.org/10.1186/s12879-021-06138-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164222PMC
May 2021

Thermal Activation of Nanoscale Wear.

Phys Rev Lett 2021 May;126(19):196101

Institute of Applied Physics, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.

Nanoscale wear tracks on ionic crystals are created by reciprocating single asperity scratch tests using atomic force microscopy. The wear characteristics are analyzed by the scratch depth as a function of surface temperature from 25 to 300 K. The average wear depth shows a nonmonotonic behavior as a function of temperature, with a transition between two different regimes characterized by the occurrence of quasiperiodic ripple formation. A thermally activated bond breaking model quantitatively explains the wear data in the low temperature, nonripple regime, but fails above the temperature threshold. This discrepancy is resolved with a geometric separation of the ripple mounds from the troughs, leading to full agreement with Arrhenius kinetics over the full temperature range.
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http://dx.doi.org/10.1103/PhysRevLett.126.196101DOI Listing
May 2021

The relationship between PLOD1 expression level and glioma prognosis investigated using public databases.

PeerJ 2021 14;9:e11422. Epub 2021 May 14.

Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Background: Glioma is the most common type of intracranial tumor with high malignancy and poor prognosis despite the use of various aggressive treatments. Targeted therapy and immunotherapy are not effective and new biomarkers need to be explored. Some Procollagen-lysine 2-oxyglutarate 5-dioxygenase () family members have been found to be involved in the metastasis and progression of tumors. Both and had been reported to be highly expressed in gliomas, while the prognostic value of remains to be further illustrated, so we want to investigate the expression in glioma and its clinical implication.

Methods: We collected gene expression and corresponding clinical data of glioma from the Chinese Glioma Genome Atlas (CGGA) database, The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) database. First, we analyzed the expression and mutation of in gliomas and its relationship with clinicopathologic characteristics. Then, we conducted survival analysis, prognostic analysis and nomogram construction of the gene. Finally, we conducted gene ontology (GO) enrichment analysis and gene set enrichment analysis (GSEA) to explore possible mechanisms and gene co-expression analysis was also be performed.

Results: The results showed that the expression level of was higher in gliomas than normal tissues, and high expression of was related to poor survival which can serve as an oncogenic factor and an independent prognostic indicator for glioma patients. Both the GO and GSEA analysis showed high expression of were enriched in Extracellular matrix (ECM) related pathways, the co-expression analysis revealed that was positively related to , , , , , , , , and , and high expression of these genes were also correlated to poor prognosis of glioma.

Conclusions: The results showed that high expression of leads to poor prognosis, and is an independent prognostic factor and a novel biomarker for the treatment of glioma. Furthermore, targeting is most likely a potential therapeutic strategy for glioma patients.
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http://dx.doi.org/10.7717/peerj.11422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127981PMC
May 2021

Hyaluronan (HA) Immobilized on Surfaces via Self-Assembled Monolayers of HA-Binding Peptide Modulates Endothelial Cell Spreading and Migration through Focal Adhesion.

ACS Appl Mater Interfaces 2021 Jun 26;13(22):25792-25804. Epub 2021 May 26.

School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.

The extracellular matrix (ECM) modulates a multitude of cell functions, and this regulation is provided by key ECM components forming a complex network. Hyaluronic acid (HA) is an abundant component of the ECM that binds to proteins and influences various activities of endothelial cells (ECs). Although the effect of soluble HA on cell spreading has been studied, the impact of peptide-bound HA has not yet been investigated in great detail. We aim to comprehensively study the roles of immobilized HA on the regulation of EC behavior compared to the more conventional use of soluble HA. A 2D model surface formed by self-assembled monolayers (SAMs) of a HA-binding peptide (Pep-1) is used as an anchor for HA immobilization. Mixed SAMs, consisting of thiolated Pep-1 and 1-octanethiol, are prepared and characterized by using ellipsometry and contact angle measurement. Full density Pep-1 SAMs are more hydrophilic and bind more HA than mixed SAMs. Cell spreading and migration are enhanced by immobilized low molecular weight (LMW) HA, which also facilitates cell alignment and elongation under laminar flow conditions and potentially drives directional migration. This effect is not mediated by the expression of CD44, and immobilized LMW HA is found to accelerate the assembly of focal adhesions. Such biomimetic surfaces provide new insights into the role of HA in regulating the spreading and phenotype of endothelial cells.
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http://dx.doi.org/10.1021/acsami.1c05574DOI Listing
June 2021

Analysis of the coverage of inactivated enterovirus 71 (EV71) vaccine and adverse events following immunization with the EV71 vaccine among children from 2016 to 2019 in Guangzhou.

Expert Rev Vaccines 2021 Jun 7:1-12. Epub 2021 Jun 7.

Ministry of immunization, Guangzhou Center For Disease Control And Prevention.

: Since 2016, China has approved the use of inactivated enterovirus 71 (EV71) vaccines produced by three manufacturers. The coverage and safety of different EV71 vaccines need to be evaluated.: The EV71 vaccination and AEFI data were collected from the Guangzhou Children's Vaccination Information Report Management System and the China AEFI Monitoring Information Management System, and the EV71 vaccine coverage rate and the AEFI incidence rate were analyzed.: From 2016 to 2019, the number of people who should have been vaccinated in Guangzhou was 2,781,618, and the coverage rates for doses 1 and 2 were 24.71% and 19.44%, respectively. The inoculation rates of vaccines from manufacturers A and B were between 3.03 and 10.46%. The reported incidence of AEFIs was 11.97 per 100,000 (147 cases), with fever (106 cases, 8.63 per 100,000) and allergic rash (59 cases, 4.80 per 100,000) being the most common reactions. There were no differences in the AEFI responses to the EV71 vaccines from the three manufacturers.: The EV71 vaccines from the three manufacturers have good safety, but the EV71 vaccine coverage rate is low. It is recommended that vaccine publicity be strengthened and that the vaccine coverage rate in children be increased.
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http://dx.doi.org/10.1080/14760584.2021.1933451DOI Listing
June 2021

Alizarin and Purpurin from L. Suppress Insulin Fibrillation and Reduce the Amyloid-Induced Cytotoxicity.

ACS Chem Neurosci 2021 06 25;12(12):2182-2193. Epub 2021 May 25.

Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin University, Tianjin 300072, P. R. China.

Alizarin (1,2-dihydroxyanthraquinone) and purpurin (1,2,4-trihydroxyanthraquinone), natural anthraquinone compounds from L., are reported to have diverse biological effects including antibacterial, antitumor, antioxidation, and so on, but the inhibition activity against amyloid aggregation has been rarely reported. In this study, we used insulin as a model protein to explore the anti-amyloid effects of the two compounds. The results showed that alizarin and purpurin inhibited the formation of insulin fibrils in a dose-dependent manner and reduced insulin-induced cytotoxicity. Meanwhile, purpurin had a more significant inhibitory effect on insulin amyloid fibrils compared with alizarin. In addition, computer simulations indicated that the two compounds interacted mainly with the hydrophobic residues of insulin chain B and interfered with the binding of phenylalanine residues. The research indicated that natural anthraquinone compounds had potential effects in preventing protein misfolding diseases and could be further used to design effective antiamyloidosis compounds.
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http://dx.doi.org/10.1021/acschemneuro.1c00177DOI Listing
June 2021

Cardiovascular magnetic resonance image analysis and mechanism study for the changes after treatments for primary microvascular angina pectoris.

Medicine (Baltimore) 2021 May;100(21):e26038

Zhejiang Hospital of Integrated Traditional Chinese and Western Medicine (HangZhou Red Cross Hospital), Hangzhou, Zhejiang, China.

Abstract: Most cases of primary microvascular angina pectoris (PMVA) are diagnosed clinically, but the etiology and pathological mechanisms are unknown. The effect of routine clinical medications is minimal, and PMVA can progress to serious cardiovascular events. To improve the diagnosis and effective treatment of this disease, this study was designed to diagnose PMVA via cardiovascular magnetic resonance (CMR) and the coronary angiography thrombolysis in myocardial infarction (TIMI) blood flow grade, as well as to analyze vascular endothelial function to elucidate the pathogenesis of PMVA and compare the effects of routine clinical medications.The present randomized controlled trial including a parallel control group will be conducted on 63 PMVA patients in our cardiovascular department. The patients will be selected and randomly divided into the control, diltiazem, and nicorandil groups. The control group will be administered routine drug treatments (aspirin, atorvastatin, betaloc ZOK, perindopril, and isosorbidemononitrate sustained-release tablets). The diltiazem group will be additionally treated with 90 mg qd diltiazem sustained-release capsules. The nicorandil group was additionally given 5 mg tid nicorandil tablets. Coronary angiography will be performed before treatment, the severity and frequency of chest pain will be evaluated before and after 9 months of treatment, and homocysteine and von Willebrand factor levels will be measured. Electrocardiography, echocardiography, dynamic electrocardiography, a treadmill exercise test, and CMR will be performed. Sex, age, body mass index, complications, smoking, and family history will also be recorded. The SPSS19.0 statistical software package will be used to analyze the data. The measurements will be expressed as the mean ± standard deviation. Measurement data will be compared between the groups using Student's t-test. A relative number description will be used for the counting data, and the chi-squaretest will be used to compare the groups. A multivariate logistic regression analysis will be performed A P-value < .05 will be considered significant.The direct indices (CMR and coronary angiographic TIMI blood flow grade) may improve after adding diltiazem or nicorandil during routine drug treatments (such as aspirin, statins, and nitrates) in PMVA patients, and indirect indices (homocysteine and von Willebrand factor levels) may be reduced.

Trial Registration: Chinese Clinical Trial Registry (http://www.chictr.org.cn/showprojen.aspx?proj=41894), No. CHiCTR1900025319, Registered on August 23, 2019; pre initiation.
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http://dx.doi.org/10.1097/MD.0000000000026038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154500PMC
May 2021

Investigation of Macular Choroidal Thickness and Blood Flow Change by Optical Coherence Tomography Angiography After Posterior Scleral Reinforcement.

Front Med (Lausanne) 2021 29;8:658259. Epub 2021 Apr 29.

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China.

This work aimed to study the effect of posterior scleral reinforcement (PSR) on choroidal thickness (CT) and blood flow. This study included 25 eyes of 24 patients with high myopia ( ≤ -6.0 dioptres or axial length ≥ 26.0 mm) who underwent PSR surgery. All patients completed the 1-month follow-up visit. Myopic macular degeneration (MMD) was graded according to the International Meta-Analysis for Pathologic Myopia (META-PM) classification based on color fundus photographs. Swept-source optical coherence tomography angiography (SSOCTA) was performed to investigate CT, choroidal perfusion area (CPA), and choriocapillaris perfusion area (CCPA) change following PSR surgery. The distribution of MMD categories was 9 (36.0%) in category 1, 10 (40.0%) in category 2, and 6 (24.0%) in category 3 or 4. MMD severity was strongly correlated with CT (all < 0.01) and CPA (all < 0.04). Postoperative CT at each sector increased significantly at 1 week's follow-up, compared to preoperative measures (all < 0.05). Postoperative CPA at subfoveal, superior, inferior, and nasal sectors also increased significantly 1 week after PSR surgery (all < 0.05). Moreover, the increased CT, CPA, and CCPA remain after PSR surgery at 1 month's follow-up, but the difference was not statistically significant. We demonstrated that the CT and choroidal blood flow increased significantly in patients with high myopia who underwent PSR surgery in a short period of time. In addition, the CT and CPA were independently associated with MMD. However, whether the transient improvement of the choroidal circulation could prevent long-term progression of high myopia warrants further study in the future.
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http://dx.doi.org/10.3389/fmed.2021.658259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130341PMC
April 2021

Phosphoproteomic Profiling of Rat's Dorsal Root Ganglia Reveals mTOR as a Potential Target in Bone Cancer Pain and Electro-Acupuncture's Analgesia.

Front Pharmacol 2021 29;12:593043. Epub 2021 Apr 29.

Key Laboratory of Acupuncture and Neurology of Zhejiang Province, Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.

Bone cancer pain (BCP) is a clinical refractory mixed pain involving neuropathic and inflammatory pain, with the underlying mechanisms remaining largely unknown. Electro-acupuncture (EA) can partly alleviate BCP according to previous research. We aim to explore the proteins and major pathways involved in BCP and EA treatment through phosphoproteomic profiling. BCP rat model was built by tibial inoculation of MRMT-1 mammary gland carcinoma cells. Mechanical hyperalgesia determined by paw withdrawal thresholds (PWTs) and bone destruction manifested on the radiographs confirmed the success of modeling, which were attenuated by EA treatment. The differentially expressed phosphorylated proteins (DEPs) co-regulated by BCP modeling and EA treatment in rat dorsal root ganglions (DRGs) were analyzed through PEX100 Protein microarray. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEPs were significantly enriched in mammalian target of rapamycin (mTOR) signaling pathway. The phosphorylations of mTOR at Ser2448 and Thr2446 were increased in BCP and downregulated by EA. In addition, the phosphorylation of S6K and Akt, markers of the mTOR complex, were also increased in BCP and downregulated by EA. Inhibition of mTOR signaling alleviated the PWTs of BCP rats, while the mTOR agonist impaired the analgesic effect of EA. Thus, our study provided a landscape of protein phosphorylation changes in DRGs of EA-treated BCP rats and revealed that mTOR signaling can be potentially targeted to alleviate BCP by EA treatment.
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http://dx.doi.org/10.3389/fphar.2021.593043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117331PMC
April 2021

Association between blood transfusion and ventilator-associated events: a nested case-control study.

Infect Control Hosp Epidemiol 2021 May 17:1-6. Epub 2021 May 17.

Chinese Evidence-based Medicine Center and CREAT Group, West China Hospital, Sichuan University, Chengdu, China.

Objectives: The association between blood transfusion and ventilator-associated events (VAEs) has not been fully understood. We sought to determine whether blood transfusion increases the risk of a VAE.

Design: Nested case-control study.

Setting: This study was based on a registry of healthcare-associated infections in intensive care units at West China Hospital system.

Patients: 1,657 VAE cases and 3,293 matched controls were identified.

Methods: For each case, 2 controls were randomly selected using incidence density sampling. We defined blood transfusion as a time-dependent variable, and we used weighted Cox models to calculate hazard ratios (HRs) for all 3 tiers of VAEs.

Results: Blood transfusion was associated with increased risk of ventilator-associated complication-plus (VAC-plus; HR, 1.47; 95% CI, 1.22-1.77; P <.001), VAC-only (HR, 1.29; 95% CI, 1.01-1.65; P = .038), infection-related VAC-plus (IVAC-plus; HR, 1.78; 95% CI, 1.33-2.39; P < .001), and possible ventilator-associated pneumonia (PVAP; HR, 2.10; 95% CI, 1.10-3.99; P = .024). Red blood cell (RBC) transfusion was also associated with increased risk of VAC-plus (HR, 1.34; 95% CI, 1.08-1.65; P = .007), IVAC-plus (HR, 1.70; 95% CI, 1.22-2.36; P = .002), and PVAP (HR, 2.49; 95% CI, 1.17-5.28; P = .018). Compared to patients without transfusion, the risk of VAE was significantly higher in patients with RBC transfusions of >3 units (HR, 1.73; 95% CI, 1.25-2.40; P = .001) but not in those with RBC transfusions of 0-3 units.

Conclusion: Blood transfusions were associated with increased risk of all tiers of VAE. The risk was significantly higher among patients who were transfused with >3 units of RBCs.
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http://dx.doi.org/10.1017/ice.2021.178DOI Listing
May 2021