Publications by authors named "Wen Luo"

371 Publications

Andrographolide Against Lung Cancer-New Pharmacological Insights Based on High-Throughput Metabolomics Analysis Combined with Network Pharmacology.

Front Pharmacol 2021 21;12:596652. Epub 2021 Apr 21.

Department of Respiratory and Critical Care, First Affiliated Hospital, Harbin Medical University, Harbin, China.

Andrographolide (Andro) has known to treat various illnesses such as colds, diarrhea, fever and infectious diseases. However, the effect mechanism of Andro is still unclear. Therefore, we used high-throughput metabolomics analysis to discover biomarkers, metabolic profiles and pathways to reveal the pharmacological action and effective mechanism of Andro against lung cancer. The metabolic effects of Andro on lung cancer animal was explored by ultra-performance liquid chromatography-triple-time of flight/mass spectrometry (UPLC-TOF/MS) analysis. Our results showed that Andro exhibited significant protective effects against lung cancer. Compared with control group, a total of 25 metabolites biomarkers was identified in urine of model animals, which 18 of them were regulated toward the normal direction after Andro treatment, and network pharmacology analysis showed that they were related with 570 proteins. Biological pathways analysis showed that the 11 metabolism pathways were regulated by Andro treatment in lung cancer mouse, and amino acid metabolism and arachidonic acid metabolism have great potential as target pathways for Andro against lung cancer. It revealed that high-throughput metabolomics combined with network pharmacology analysis provides deeply insight into the therapeutic mechanisms of natural product for promoting medicine development and disease treatment.
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http://dx.doi.org/10.3389/fphar.2021.596652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097142PMC
April 2021

Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation.

Biochem Biophys Res Commun 2021 Apr 29;559:84-91. Epub 2021 Apr 29.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Skeletal muscle development is a sophisticated multistep process orchestrated by diverse myogenic transcription factors. Recent studies have suggested that Kelch-like genes play vital roles in muscle disease and myogenesis. However, it is still unclear how Kelch-like genes impact myoblast physiology. Here, through integrative analysis of the mRNA expression profile during chicken primary myoblast and C2C12 differentiation, many differentially expressed genes were found and suggested to be enriched in myoblast differentiation and muscle development. Interestingly, a little-known Kelch-like gene KLHL30 was screened as skeletal muscle-specific gene with essential roles in myogenic differentiation. Transcriptomic data and quantitative PCR analysis indicated that the expression of KLHL30 is upregulated under myoblast differentiation state. KLHL30 overexpression upregulated the protein expression of myogenic transcription factors (MYOD, MYOG, MEF2C) and induced myoblast differentiation and myotube formation, while knockdown of KLHL30 caused the opposite effect. Furthermore, KLHL30 was found to significantly decrease the numbers of cells in the S stage and thereby depress myoblast proliferation. Collectively, this study highlights that KLHL30 as a muscle-specific regulator plays essential roles in myoblast proliferation and differentiation.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.086DOI Listing
April 2021

Discovery of 2-(3-(3-Carbamoylpiperidin-1-yl)phenoxy)acetic Acid Derivatives as Novel Small-Molecule Inhibitors of the β-Catenin/B-Cell Lymphoma 9 Protein-Protein Interaction.

J Med Chem 2021 May 26;64(9):5886-5904. Epub 2021 Apr 26.

Drug Discovery Department, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612-9497, United States.

The β-catenin/B-cell lymphoma 9 (BCL9) protein-protein interaction (PPI) is a potential target for the suppression of hyperactive Wnt/β-catenin signaling that is vigorously involved in cancer initiation and development. Herein, we describe the medicinal chemistry optimization of a screening hit to yield novel small-molecule inhibitors of the β-catenin/BCL9 interaction. The best compound can disrupt the β-catenin/BCL9 interaction with a of 3.6 μM in AlphaScreen competitive inhibition assays. Cell-based experiments revealed that selectively disrupted the β-catenin/BCL9 PPI, while leaving the β-catenin/E-cadherin PPI unaffected, dose-dependently suppressed Wnt signaling transactivation, downregulated oncogenic Wnt target gene expression, and on-target selectively inhibited the growth of cancer cells harboring aberrant Wnt signaling. This compound with a new chemotype can serve as a lead compound for further optimization of inhibitors for β-catenin/BCL9 PPI.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00046DOI Listing
May 2021

Orthogonal Reconstruction of Upconversion and Holographic Images for Anticounterfeiting Based on Energy Transfer.

ACS Appl Mater Interfaces 2021 Apr 20;13(16):19159-19167. Epub 2021 Apr 20.

Key Lab for Material Chemistry of Energy Conversion and Storage, Ministry of Education, and Hubei Key Lab of Materials Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Crosstalk-free reconstruction of multiple images within a single element can greatly boost the image capacity and information security. We herein demonstrate a viable approach by integrating upconversion and holographic images into a single holographic polymer nanocomposite. The holographic image is reconstructed through photopolymerization-induced phase separation under a 460 nm laser and identifiable under room light, while the upconversion image recognizable under a 980 nm laser is photopatterned via spatially photobleaching of the dye embedded in the upconversion nanoparticle (UCNP) shell under 365 nm light. To this end, the lanthanide-doped UCNP in the core/shell/shell nanostructure of NaYF:20%Yb,0.5%Tm@NaYF@SiO is designed, and the dye, fluorescein isothiocyanate (FITC), is fixed in the outermost SiO shell via the amine-isothiocyanate reaction and the subsequent sol-gel reaction. Energy transfer from the core of the UCNP to FITC embedded in the shell is critical to boosting the contrast of the upconversion image, which dials the emission color from blue to yellow-green. It is also found that the upconversion image can be brightened by increasing the UCNP content while the holographic image is weakened when the UCNP content is over 15 wt %. This study paves a new way toward advanced anticounterfeiting.
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http://dx.doi.org/10.1021/acsami.1c02561DOI Listing
April 2021

Characterization of Chicken Skin Yellowness and Exploration of Genes Involved in Skin Yellowness Deposition in Chicken.

Front Physiol 2021 31;12:585089. Epub 2021 Mar 31.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China.

Skin color is an important economic trait in meat-type chickens. A uniform bright skin color can increase the sales value of chicken. Chickens with bright yellow skin are more popular in China, especially in the broiler market of South China. However, the skin color of chickens can vary because of differences in breeds, diet, health, and individual genetics. To obtain greater insight into the genetic factors associated with the process of skin pigmentation in chickens, we used a colorimeter and high-resolution skin photographs to measure and analyze the skin color of chickens. By analyzing 534 chickens of the same breed, age, and feed condition, we found that the yellowness values of the chickens varied within this population. A significant positive correlation was found between the cloacal skin yellowness values before and after slaughter, and the cloacal skin yellowness value of live chickens was positively correlated with the overall body skin yellowness value. Additionally, chicken skin yellowness exhibited low heritability, ranging from 0.07 to 0.27. Through RNA sequencing, 882 genes were found to be differentially expressed between the skin with the highest and lowest yellowness values. Some of these differentially expressed genes may play an important role in yellow pigment deposition in chicken skin, which included , and . In addition, we found that the expression and variations of the gene, which is referred to as the yellow skin gene, cannot be used to estimate the skin yellowness value of chickens in this population. These data will help to further our understanding of chicken skin yellowness and might contribute to the selection of specific chicken strains with consistent skin coloration.
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http://dx.doi.org/10.3389/fphys.2021.585089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044320PMC
March 2021

Insights into the co-doping effect of Fe and Zr on the anti-K performance of CeTiO catalyst for NH-SCR reaction.

J Hazard Mater 2021 Apr 8;416:125821. Epub 2021 Apr 8.

Research Center for Atmospheric Environment, Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China; College of Resources and Environment, Chongqing School, University of Chinese Academy of Sciences (UCAS Chongqing), Chongqing 400714, PR China; School of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China.

A novel K-resistant Fe and Zr co-doped CeTiO catalyst was first prepared by co-precipitation method for the ammonia-selective catalytic reduction (NH-SCR) of NO. On the premise of retaining the outstanding catalytic activity of CeTiO catalyst, Fe and Zr co-doping efficiently improves its K-resistance with superior NO conversion up to 84% after K-poisoning. Specially, the grain growth during the second calcination after K poisoning is successfully inhibited by Fe and Zr co-doping. Consequently, the large specific surface area with increased acid sites and efficiently retained reducibility over K-poisoned FeZrCeTiO catalyst are realized, which prompt NH activation and NO oxidation, further benefit NH-SCR. Besides, NH-SCR reaction over CeTiO and FeZrCeTiO catalysts follows a possible L-H mechanism, and K-poisoning makes no change to it. Finally, a reasonable anti-K poisoning mechanism of FeZrCeTiO catalyst is proposed: the excellent K-resistance is attributed to part of Fe and Zr are sacrificed to form Fe-O-K and Zr-O-K species protecting the active site Ce-O-Ti from K-poisoning, as well as the additional reducibility and surface acidity brought from Fe-O species with Zr prompting its uniform distribution.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125821DOI Listing
April 2021

Constructing Three-Dimensional Macroporous TiO Microspheres with Enhanced Pseudocapacitive Lithium Storage under Deep Discharging/Charging Conditions.

ACS Appl Mater Interfaces 2021 Apr 1;13(14):16528-16535. Epub 2021 Apr 1.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, International School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, P. R. China.

TiO has been intensively investigated as an anode material for lithium-ion batteries (LIBs) in 1.0-3.0 V ( Li/Li). However, it is a challenge to realize its theoretical capacity (336 mAh g) in this limited potential range. Extending the potential range below 1.0 V would increase its capacity but usually at the expense of its cyclic stability owing to the sluggish ionic diffusion and unsatisfactory structural stability. Here, three-dimensional (3D) macroporous TiO microspheres with interconnected pores and nanocrystalline thin walls have been constructed through a scalable template-assisted spray drying method to overcome these obstacles. When applied to LIBs, high and stable discharge capacity (300 mAh g at 0.1 A g) as well as superior cyclic stability (242 mAh g after 1000 cycles at 1.0 A g) can be achieved under deep discharging/charging conditions (0.01-3.0 V Li/Li). Furthermore, the 3D macroporous structure is well preserved under deep discharging/charging and the X-ray diffraction (XRD) patterns and Raman spectra reveal the dominant pseudocapacitive contribution at low potentials (0.01-1.0 V). This work not only develops a facile method to synthesize macroporous metal oxides but also provides insight into the lithium storage mechanism of TiO under deep discharging/charging conditions.
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http://dx.doi.org/10.1021/acsami.1c02411DOI Listing
April 2021

Validation of the Chinese Version of the Patient's Communication Perceived Self-Efficacy Scale (PCSS) in Outpatients After Total Hip Replacement.

Patient Prefer Adherence 2021 19;15:625-633. Epub 2021 Mar 19.

The 2nd Ward of Joint Surgery, Tianjin Hospital, Tianjin, People's Republic of China.

Background: Among older people in the world, older patients' communication has become a public health issue of vital importance. Such communication could be improved by different interventions. However, a means of measuring patient's communication confidence in these measures has not been established in China. This study is aimed at translating and introducing the Patient's Communication Self-Efficacy Scale for communication between doctors and patients after total hip replacement.

Method: (1) A questionnaire was completed after a consultation by 167 patients (mean age = 70.04 years; SD: 6.3 years; females/males: 94/73). Translation of the original English version PCSS into the Chinese; (2) Validation of the final Chinese version of the PCSS. Measurement indexes included item generation, reliability testing, construct validity and test-retest reliability. To actualize the above test, we used SPSS 19.0 software and LISREL 8.7. We build the Bayesian Network Model of the Chinese version of the PCSS and determined predictive variables.

Result: Confirmatory factor analysis showed that the Chinese version of the PCSS has fit a three-dimensional model. Meanwhile, the Chinese version of the PCSS has high internal consistency (Cronbach's α coefficient 0.929) and test-retest reliability (Kappa coefficient 0.761). Analysis using Bayesian networks shows that the important predictors are education (0.4207), PEPPI 3(0.3951), and PCSS 1(0.1139). The connections between PCSS 3 and other variables do not indicate causality, conditional dependencies or inter-relatedness.

Conclusion: This is the first study to validate the Chinese version of the PCSS in outpatients after total hip replacement. Our results confirmed that the Chinese version of the scale has high internal consistency, construct validity and test-retest reliability. And the patient-doctor interaction and education are important predictors of patient's communication self-efficacy.
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http://dx.doi.org/10.2147/PPA.S301670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989552PMC
March 2021

Hydrogen Storage by Reduction of CO₂ to Synthetic Hydrocarbons.

Chimia (Aarau) 2021 Mar;75(3):156-162

Laboratory of Materials for Renewable Energy (LMER), Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Feacute;dérale de Lausanne, EPFL Valais/Wallis, Switzerland and Empa Materials Science and Technology, Dübendorf, Switzerland.

The storage of renewable energy is crucial for the substitution of fossil fuels with renewable energy. Hydrogen is the first step in the conversion of electricity from renewable sources to an energy carrier. However, hydrogen is technically and economically challenging to store, but can be converted with CO₂ from the atmosphere or oceans to hydrocarbons. The heterogeneously catalyzed gas phase reaction and the electrochemical CO₂ reduction are reviewed and the application of a new type of reactor is described. The mechanism of the gas phase CO₂ reduction on a heterogeneous catalyst is shown in detail and the function of the supported catalyst is explained. Finally, an economic estimation on the cost of synthetic methane is presented which leads to a cost of 0.3 CHF/kWh in CH₄.
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http://dx.doi.org/10.2533/chimia.2021.156DOI Listing
March 2021

lncRNA‑MIAT facilitates the differentiation of adipose‑derived mesenchymal stem cells into lymphatic endothelial cells via the miR‑495/Prox1 axis.

Mol Med Rep 2021 May 24;23(5). Epub 2021 Mar 24.

Department of Breast Tumor Plastic Surgery (Third Department of Head and Neck Surgery), Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya Medical School, Central South University, Changsha, Hunan 410013, P.R. China.

The development of novel treatments for lymphedema is hindered by the poorly understood pathophysiology of the disease. To improve the therapeutic success of treating the disease, the present study aimed to investigate the effects and mechanism of long non‑coding RNA myocardial infarction‑associated transcript (MIAT) in terms of the differentiation of adipose‑derived mesenchymal stem cells (ADMSCs) into lymphatic endothelial cells (LECs). The expression levels of (MIAT), microRNA (miR)‑495 and Prospero‑related homeobox 1 (Prox1) were measured by reverse transcription‑quantitative PCR. The protein expression levels of Prox1, lymphatic vessel endothelial hyaluronan receptor 1 (LYVE‑1), vascular endothelial growth factor receptor‑3 (VEGFR‑3) and podoplanin (PDPL) were detected by western blotting and immunofluorescence. A dual‑luciferase reporter assay was also used to detect the interaction between MIAT, miR‑495 and Prox1. In addition, migration and tube‑formation capabilities were measured by Transwell assay and tube‑formation assay, respectively. The results obtained demonstrated that VEGF‑C156S (recombinant VEGF‑C in which Cys156 was replaced by Ser residue) treatment could efficiently induce the differentiation of ADMSCs into LECs. MIAT expression was upregulated and miR‑495 was downregulated during differentiation. Mechanistically, MIAT upregulated Prox1 expression possibly by acting as a molecular sponge for miR‑495. Functional analyses indicated that the expression levels of Prox1, LYVE‑1, VEGFR‑3 and PDPL, and the migration and tube‑formation capabilities of ADMSCs induced by VEGF‑C156S, were significantly inhibited by silencing MIAT and overexpressing miR‑495. Moreover, miR‑495 inhibition and Prox1 overexpression reversed the effects of MIAT downregulation and miR‑495 upregulation, respectively, on the differentiation of ADMSCs into LECs. Taken together, these results suggested that MIAT may be involved in the differentiation of ADMSCs into LECs, and that the MIAT/miR‑495/Prox1 axis may be a novel regulatory mechanism and therapeutic target for the treatment of lymphedema.
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http://dx.doi.org/10.3892/mmr.2021.11962DOI Listing
May 2021

Regulating Lattice-Water-Adsorbed Ions to Optimize Intercalation Potential in 3D Prussian Blue Based Multi-Ion Microbattery.

Small 2021 May 21;17(18):e2007791. Epub 2021 Mar 21.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China.

Miniaturized energy storage device (MESD) is the core module in microscale electronic equipment, yet its electrochemical performance is far away from the actual requirements. The extensive research efforts have improved the performance of MESD via the fabrication techniques and material construction, while ignoring the expansion of optimization strategy in the combination of energy storage mechanism. Herein, the Prussian blue/Zn microbattery is reported with the regulation of lattice-water-adsorbed intercalated ion. The optimal charge transport of cathode is achieved via the optimization of 3D structure of microelectrode to maximize the electrochemical performance. Also, lattice-water-adsorbed ion storage mechanism is further investigated to guide the design of differential energy storage for cathode and anode. The Cu (Fe(CN) ) /Zn microbattery, with K inter/deintercalation in the cathode and Zn deplating/plating in the anode, displays high capacity (0.281 mAh cm at 2.5 mA cm ), rate performance (0.181 mAh cm at 25 mA cm ), and cycling stability (77.6% capacity retention after 1500 cycles) enhanced by Cu in the electrolyte. This highly efficient combination of fabrication process, active material, and multi-ion storage for microelectrode shows a high tolerance for optimization strategies, expanding the compatibility of optimization path for high-performance MESD.
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http://dx.doi.org/10.1002/smll.202007791DOI Listing
May 2021

Custom-Made Antibiotic Cement-Coated Nail for the Treatment of Infected Bone Defect.

Biomed Res Int 2021 5;2021:6693906. Epub 2021 Mar 5.

Department of Orthopaedics, Second Affiliated Hospital, Air Force Medical University, Xi'an, Shaanxi 710038, China.

Background: Longbone infected bone defect remains a great challenge due to multiple surgeries, long-term treatment duration, and uncertain prognosis. Treatment principles include eradication/debridement, stabilization, and antibiotic administration. An antibiotic cement-coated nail has shown great prospects due to both local antibiotic elution and stabilization of bone defects. However, the current fabrication technique remains to be improved.

Methods: For the first time, we described a new method for custom-made cement-coated nail fabrication based on a 3D printing technique. A retrospective study of 19 consecutive patients with long bone infected bone defects from one medical center was conducted who met the inclusion and exclusion criteria from November 2016 to May 2020. The treatment involved thorough debridement, custom-made antibiotic cement-coated nail filling, and culture-specific systemic antibiotic treatment guided by a multidisciplinary team. Clinical and radiographic examinations (X-ray and CT scans) were used to evaluate bony union. Clinical and laboratory examinations were used to evaluate the infection control. The SF-36 score was used to evaluate patients' quality of life pre- and postoperatively.

Results: The mean follow-up was 98.8 weeks (ranging from 40 to 192). All cases achieved infection control, 3 cases achieved bone healing after one-stage operation, and 12 cases achieved bone healing after a two-stage bone graft procedure. At the last follow-up, none of the 19 patients had infection recurrence or 1 case had failure of the protective plate. The pre- and postoperative SF-36 score showed that there were statistical differences in all the 9 aspects.

Conclusions: The precise custom-made antibiotic cement-coated intramedullary nail through the 3D printing technique used in this study is an effective strategy for the treatment of infected bone defects of long bone. This technique may help to increase the infection control rate and promote bone healing.
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http://dx.doi.org/10.1155/2021/6693906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954627PMC
March 2021

Query the trajectory based on the precise track: a Bloom filter-based approach.

Geoinformatica 2021 Mar 15:1-20. Epub 2021 Mar 15.

Ministry of Education, Key Laboratory of Virtual Geographic Environment (Nanjing Normal University), Nanjing, China.

Fast and precise querying in a given set of trajectory points is an important issue of trajectory query. Typically, there are massive trajectory data in the database, yet the query sets only have a few points, which is a challenge for the superior performance of trajectory querying. The current trajectory query methods commonly use the tree-based index structure and the signature-based method to classify, simplify, and filter the trajectory to improve the performance. However, the unstructured essence and the spatiotemporal heterogeneity of the trajectory-sequence lead these methods to a high degree of spatial overlap, frequent I/O, and high memory occupation. Thus, they are not suitable for the time-critical tasks of trajectory big data. In this paper, a query method of trajectory is developed on the Bloom Filter. Based on the gridded space and geocoding, the spatial trajectory sequences (tracks) query is transformed into the query of the text string. The geospace was regularly divided by the geographic grid, and each cell was assigned an independent geocode, converting the high-dimensional irregular space trajectory query into a one-dimensional string query. The point in each cell is regarded as a signature, which forms a mapping to the bit-array of the Bloom Filter. This conversion effectively eliminates the high degree of overlap and instability of query performance. Meanwhile, the independent coding ensures the uniqueness of the whole tracks. In this method, there is no need for additional I/O on the raw trajectory data when the track is queried. Compared to the original data, the memory occupied by this method is negligible. Based on Beijing Taxi and Shenzhen bus trajectory data, an experiment using this method was constructed, and random queries under a variety of conditions boundaries were constructed. The results verified that the performance and stability of our method, compared to R*tree index, have been improved by 2000 to 4000 times, based on one million to tens of millions of trajectory data. And the Bloom Filter-based query method is hardly affected by grid size, original data size, and length of tracks. With such a time advantage, our method is suitable for time-critical spatial computation tasks, such as anti-terrorism, public safety, epidemic prevention, and control, etc.
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http://dx.doi.org/10.1007/s10707-021-00433-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957281PMC
March 2021

High-efficiency water-window x-ray generation from nanowire array targets irradiated with femtosecond laser pulses.

Opt Express 2021 Feb;29(4):5427-5436

We demonstrate the high-efficiency generation of water-window soft x-ray emissions from polyethylene nanowire array targets irradiated by femtosecond laser pulses at the intensity of 4×10 W/cm. The experimental results indicate more than one order of magnitude enhancement of the water-window x-ray emissions from the nanowire array targets compared to the planar targets. The highest energy conversion efficiency from laser to water-window x-rays is measured as 0.5%/sr, which comes from the targets with the longest nanowires. Supported by particle-in-cell simulations and atomic kinetic codes, the physics that leads to the high conversion efficiency is discussed.
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http://dx.doi.org/10.1364/OE.417512DOI Listing
February 2021

Pharmacological Inhibition of Brain EGFR Activation By a BBB-penetrating Inhibitor, AZD3759, Attenuates α-synuclein Pathology in a Mouse Model of α-Synuclein Propagation.

Neurotherapeutics 2021 Mar 12. Epub 2021 Mar 12.

McGill Parkinson Program and Neurodegenerative Diseases Group, Montreal Neurological Institute, McGill University, Montréal, QC, Canada.

Aggregation and deposition of α-synuclein (α-syn) in Lewy bodies within dopamine neurons of substantia nigra (SN) is the pathological hallmark of Parkinson's disease (PD). These toxic α-syn aggregates are believed to propagate from neuron-to-neuron and spread the α-syn pathology throughout the brain beyond dopamine neurons in a prion-like manner. Targeting propagation of such α-syn aggregates is of high interest but requires identifying pathways involving in this process. Evidence from previous Alzheimer's disease reports suggests that EGFR may be involved in the prion-like propagation and seeding of amyloid-β. We show here that EGFR regulates the uptake of exogenous α-syn-PFFs and the levels of endogenous α-syn in cell cultures and a mouse model of α-syn propagation, respectively. Thus, we tested the therapeutic potentials of AZD3759, a highly selective BBB-penetrating EGFR inhibitor, in a preclinical mouse model of α-syn propagation. AZD3759 decreases activated EGFR levels in the brain and reduces phosphorylated α-synuclein (pSyn) pathology in brain sections, including striatum and SN. As AZD3759 is already in the clinic, this paper's results suggest a possible repositioning of AZD3759 as a disease-modifying approach for PD.
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http://dx.doi.org/10.1007/s13311-021-01017-6DOI Listing
March 2021

Biocompatibility nanoprobe of MXene N-TiC quantum dot/Fe for detection and fluorescence imaging of glutathione in living cells.

Colloids Surf B Biointerfaces 2021 May 18;201:111631. Epub 2021 Feb 18.

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address:

MXene quantum dots have attracted much attention due to their great optical performance and excellent water solubility. Glutathione (GSH) plays a key role in living cells. In this study, a biocompatibility nanoprobe was prepared for detecting intracellular GSH based on MXene N-TiC quantum dots (N-TiC QDs). The N-TiC QDs act as the fluorescence reporters and the ferric iron (Fe) as the quenchers based on nonradiative electron-hole annihilation. When Fe encounters the amino group of N-TiC QDs, the electrons of N-TiC QDs in the excited state will transfer to the half-filled 3d orbitals of Fe, leading to the fluorescence quenching of N-TiC QDs. When the N-TiC QDs/Fe nanoprobe acts on the cancer cell MCF-7, the abundant GSH in the cancer cells can reduce Fe to Fe, which will restore the fluorescence of N-TiC QDs. The N-TiC QDs/Fe nanoprobe displays a high sensitivity for GSH with a detection limit of 0.17 μM in range of 0.5-100 μM. It becomes a promising probe for detecting and showing cellular imaging of GSH in MCF-7 cells. The N-TiC QDs/Fe nanoprobe might provide a new way for imaging-guided precision cancer diagnosis.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111631DOI Listing
May 2021

Cytokine inducible SH2-containing protein potentiate J subgroup avian leukosis virus replication and suppress antiviral responses in DF-1 chicken fibroblast cells.

Virus Res 2021 Apr 23;296:198344. Epub 2021 Feb 23.

Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China; Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642, China. Electronic address:

Cytokine-inducible Srchomology2 (SH2)-containing protein (CIS) belongs to the suppressors of cytokine signaling (SOCS) protein family function as a negative feedback loop inhibiting cytokine signal transduction. J subgroup avian leukosis virus (ALV-J), a commonly-seen avian virus with a feature of immunosuppression, poses an unmeasurable threat to the poultry industry across the world. However, commercial medicines or vaccines are still no available for this virus. This study aims to evaluate the potential effect of chicken CIS in antiviral response and its role on ALV-J replication. The results showed that ALV-J strain SCAU-HN06 infection induced CIS expression in DF-1 cells, which was derived from chicken embryo free of endogenous avian sarcoma-leukosis virus (ASLV) like sequences. By overexpressing CIS, the expression of chicken type I interferon (IFN-I) and interferon-stimulated genes (ISGs; PKR, ZAP, CH25H, CCL4, IFIT5, and ISG12) were both suppressed. Meanwhile, data showed that CIS overexpression also increased viral yield. Interestingly, knockdown of CIS enhanced induction of IFN-I and ISGs and inhibited viral replication. Collectively, we proved that modulation of CIS expression not only affected SCAU-HN06 replication in vitro but also altered the expression of IFN-I and ISGs that act as an essential part of antiviral innate immune system. Our data provide a potential target for developing antiviral agents for ALV-J.
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http://dx.doi.org/10.1016/j.virusres.2021.198344DOI Listing
April 2021

High expression of BCL6 inhibits the differentiation and development of hematopoietic stem cells and affects the growth and development of chickens.

J Anim Sci Biotechnol 2021 Feb 5;12(1):18. Epub 2021 Feb 5.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China.

Background: B-cell CLL/lymphoma 6 (BCL6) is a transcriptional master regulator that represses more than 1200 potential target genes. Our previous study showed that a decline in blood production in runting and stunting syndrome (RSS) affected sex-linked dwarf (SLD) chickens compared to SLD chickens. However, the association between BCL6 gene and hematopoietic function remains unknown in chickens.

Methods: In this study, we used RSS affected SLD (RSS-SLD) chickens, SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells (HSCs), to investigate the effect of the BCL6 on differentiation and development of HSCs.

Results: The results showed that comparison of RSS-SLD chickens with SLD chickens, the BCL6 was highly expressed in RSS-SLD chickens bone marrow. The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow, bone density was reduced, and the levels of immature erythrocytes in peripheral blood were increased. At the same time, the hematopoietic function of HSCs decreased in RSS-SLD chickens, which was manifested by a decrease in the hematopoietic growth factors (HGFs) EPO, SCF, TPO, and IL-3, as well as hemoglobin α and hemoglobin β expression. Moreover, mitochondrial function in the HSCs of RSS-SLD chickens was damaged, including an increase in ROS production, decrease in ATP concentration, and decrease in mitochondrial membrane potential (ΔΨm). The same results were also observed in SLD chickens compared with normal chickens; however, the symptoms were more serious in RSS-SLD chickens. Additionally, after overexpression of the BCL6 in primary HSCs, the secretion of HGFs (EPO, SCF, TPO and IL-3) was inhibited and the expression of hemoglobin α and hemoglobin β was decreased. However, cell proliferation was accelerated, apoptosis was inhibited, and the HSCs entered a cancerous state. The function of mitochondria was also abnormal, ROS production was decreased, and ATP concentration and ΔΨm were increased, which was related to the inhibition of apoptosis of stem cells.

Conclusions: Taken together, we conclude that the high expression of BCL6 inhibits the differentiation and development of HSCs by affecting mitochondrial function, resulting in impaired growth and development of chickens. Moreover, the abnormal expression of BCL6 might be a cause of the clinical manifestations of chicken comb, pale skin, stunted growth and development, and the tendency to appear RSS in SLD chickens.
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http://dx.doi.org/10.1186/s40104-020-00541-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863511PMC
February 2021

Ultrasensitive detection of cyclin D1 by a self-enhanced ECL immunosensor based on BiS quantum dots.

Analyst 2021 Mar 4;146(6):2057-2064. Epub 2021 Feb 4.

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.

Bismuth sulfide quantum dots (BiS QDs), which have excellent optical and thermoelectric properties, represent a green and non-toxic semiconductor material that has been widely used in catalysis and photoelectric conversion devices. At present, research on this material has gradually expanded into the biological field. Herein, the biomineralization method mediated by bovine serum albumin (BSA) was utilized to synthesize BiS QDs with monodispersity, excellent colloidal stability, and good biocompatibility. This is the first study on the electrochemiluminescence (ECL) characteristics of BiS QDs and related ECL mechanisms in detail. In addition, on the basis of BiS QDs, an ECL immunosensor was used for the ultrasensitive measurement of cyclin D1 (CCND1). The composite material, namely Au@Cu-BiS QDs was used as a high-sensitivity ECL probe, in which AuNPs were connected with BiS QDs through a copper(ii) ion bridge. PDA-AgNPs made of dopamine (DA) and silver nanoparticles (AgNPs) were utilized as a carrier for fixing the primary antibody (Ab), ultimately presenting a relatively wide detection range of 10 fg mL-1 μg mL. Moreover, quite a low detection limit (6.34 fg mL) was also obtained for an assay of CCND1. Results indicated that the immunosensor can provide a potential platform with fine stability and creditable reproducibility for clinical diagnosis.
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http://dx.doi.org/10.1039/d0an02296aDOI Listing
March 2021

Effects of gut microbiota on atherosclerosis through hydrogen sulfide.

Eur J Pharmacol 2021 Apr 30;896:173916. Epub 2021 Jan 30.

Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan Province, University of South China, Hengyang, 421001, China. Electronic address:

Cardiovascular diseases are the leading cause of death and morbidity worldwide. Atherosclerotic cardiovascular disease (ASCVD) is affected by both environmental and genetic factors. Microenvironmental disorders of the human gut flora are associated with a variety of health problems, not only gastrointestinal diseases, such as inflammatory bowel disease, but also extralintestinal organs. Hydrogen sulfide (HS) is the third gas signaling molecule other than nitric oxide and carbon monoxide. In the cardiovascular system, HS plays important roles in the regulation of blood pressure, angiogenesis, smooth muscle cell proliferation and apoptosis, anti-oxidative stress, cardiac functions. This review is aiming to explore the potential role of gut microbiota in the development of atherosclerosis through hydrogen sulfide production as a novel therapeutic direction for atherosclerosis.
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http://dx.doi.org/10.1016/j.ejphar.2021.173916DOI Listing
April 2021

Integrative Analyses of mRNA Expression Profile Reveal and Play Important Roles in Mutation-Induced Excessive Abdominal Fat Deposition in the Sex-Linked Dwarf Chicken.

Front Genet 2020 14;11:610605. Epub 2021 Jan 14.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China.

Sex-linked dwarf (SLD) chicken, which is caused by a recessive mutation of the growth hormone receptor (), has been widely used in the Chinese broiler industry. However, it has been found that the SLD chicken has more abdominal fat deposition than normal chicken. Excessive fat deposition not only reduced the carcass quality of the broilers but also reduced the immunity of broilers to diseases. To find out the key genes and the precise regulatory pathways that were involved in the mutation-induced excessive fat deposition, we used high-fat diet (HFD) and normal diet to feed the SLD chicken and normal chicken and analyzed the differentially expressed genes (DEGs) among the four groups. Results showed that the SLD chicken had more abdominal fat deposition and larger adipocytes size than normal chicken and HFD can promote abdominal fat deposition and induce adipocyte hypertrophy. RNA sequencing results of the livers and abdominal fats from the above chickens revealed that many DEGs between the SLD and normal chickens were enriched in fat metabolic pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, extracellular matrix (ECM)-receptor pathway, and fatty acid metabolism. Importantly, by constructing and analyzing the -downstream regulatory network, we found that suppressor of cytokine signaling 2 () and cytokine-inducible SH2-containing protein () may involve in the mutation-induced abdominal fat deposition in chicken. The ectopic expression of and in liver-related cell line leghorn strain M chicken hepatoma (LMH) cell and immortalized chicken preadipocytes (ICP) revealed that these two genes can regulate fatty acid metabolism, adipocyte differentiation, and lipid droplet accumulation. Notably, overexpression of and can rescue the hyperactive lipid metabolism and excessive lipid droplet accumulation of primary liver cell and preadipocytes that were isolated from the SLD chicken. This study found some genes and pathways involved in abdominal fat deposition of the SLD chicken and reveals that and are two key genes involved in the mutation-induced excessive fat deposition of the SLD chicken.
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http://dx.doi.org/10.3389/fgene.2020.610605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841439PMC
January 2021

Radiation-induced eosinophils improve cytotoxic T lymphocyte recruitment and response to immunotherapy.

Sci Adv 2021 Jan 29;7(5). Epub 2021 Jan 29.

Chongqing Key Laboratory of Immunotherapy, Chongqing 400037, P.R. China.

The efficacy of cancer immunotherapy is dictated by CD8 T cell infiltration and the nature of the tumor microenvironment (TME). By inflaming the TME to favor CD8 T cell immunity, radiation is now widely considered as a neoadjuvant for immunomodulation. Here, we observed that local irradiation enhances the infiltration of intratumoral eosinophils, and depletion of eosinophil dampens CD8 T cell infiltration and diminishes the anti-tumor effectiveness of radiation. Retrospectively, we identified a strong correlation between eosinophilia and survival benefit in radiation-treated cancer patients. Experimentally, we further show that radiation enhances the intratumoral infiltration of adoptive transferred T cells therapy, bolstering eosinophils by intravenous interleukin-5 administration promotes the efficacy of radiation-induced abscopal effect. Together, these results suggest that eosinophil mobilization can be considered as a mechanistically relevant biomarker for predicting the effectiveness of pre-immunotherapy radiation, as well as a new strategy to enhance T cell-mediated immunotherapy against cancers.
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http://dx.doi.org/10.1126/sciadv.abc7609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846170PMC
January 2021

[Propeptide-mediated protein folding: mechanism and its impact on lipase].

Sheng Wu Gong Cheng Xue Bao 2021 Jan;37(1):88-99

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China.

The formation of most proteins consists of two steps: the synthesis of precursor proteins and the synthesis of functional proteins. In these processes, propeptides play important roles in assisting protein folding or inhibiting its activity. As an important polypeptide chain coded by a gene sequence in lipase gene, propeptide usually functions as an intramolecular chaperone, assisting enzyme molecule folding. Meanwhile, some specific sites on propeptide such as glycosylated sites, have important effect on the activity, stability in extreme environment, methanol resistance and the substrate specificity of the lipase. Studying the mechanism of propeptide-mediated protein folding, as well as the influence of propeptide on lipases, will allow to regulate lipase by alternating the propeptide folding behavior and in turn pave new ways for protein engineering research.
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http://dx.doi.org/10.13345/j.cjb.200206DOI Listing
January 2021

Glutathione and ethylene biosynthesis reveal that the glume and lemma have better tolerance to water deficit in wheat.

Plant Physiol Biochem 2021 Mar 12;160:120-129. Epub 2021 Jan 12.

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, PR China. Electronic address:

As senescence progresses, the sensitivity of wheat organs to plant hormones during the grain-filling stages cannot be ignored. Especially under water deficit situation, non-leaf organs (spikes) have better photosynthesis and drought-tolerance traits than flag leaves. However, the mechanism of ethylene synthesis in wheat organs under water deficit remains unclear. We have studied the influence of water deficit in wheat flag leaves and spike bracts on photosynthetic parameters and on the expression of key enzymes involved in the ethylene biosynthesis pathway during the late grain-filling stages. More stable chlorophyll content (Chl), maximum PSII quantum yield (Fv/Fm), nonphotochemical quenching (NPQ) and maximal efficiency of PSII photochemistry under light adaptation (Fv'/Fm') were observed in the spike bracts than that in the flag leaves during the late grain-filling stages. In addition, the activity of glutathione reductase (GR), γ-glutamylcysteine synthetase (γ-ECS), 1-aminocyclopropane-1-carboxylic (ACC) acid synthase (ACS), and ACC oxidase (ACO) induced ethylene synthesis and influenced plant growth. Further analysis of genes encoding cysteine-ethylene related proteins (γ-ECS, GR, ACO, ACS1, and ASC2) demonstrated that ear organs and flag leaves exhibited different expression patterns. These findings will facilitate future investigations of the regulatory senescence response mechanisms of cysteine interaction with ethylene in wheat under conditions of drought stress.
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http://dx.doi.org/10.1016/j.plaphy.2021.01.008DOI Listing
March 2021

Effect of Root Diameter on the Selection and Network Interactions of Root-Associated Bacterial Microbiomes in Robinia pseudoacacia L.

Microb Ecol 2021 Jan 15. Epub 2021 Jan 15.

Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, 3 Taicheng Road, Yangling, Xianyang, 712100, Shaanxi, People's Republic of China.

The high plasticity of root morphology, physiology, and function influences root-associated microbiomes. However, the variation in root-associated microbiome diversity and structures in response to root diameter at different root depths remains poorly understood. Here, we selected black locust (Robinia pseudoacacia L.) as a model plant to investigate the selection and network interactions of rhizospheric and root endophytic bacterial microbiomes associated with roots of different diameters (1, 1-2, and > 2 mm) among root depths of 0-100 cm via the Illumina sequencing of the 16S rRNA gene. The results showed that the alpha diversity of the root-associated bacterial communities decreased with increasing root diameters among different root depths; fewer orders with higher relative abundance, especially in the endosphere, were enriched in association with coarse roots (> 2 mm) than fine roots among root depths. Furthermore, the variation in the enriched bacterial orders associated with different root diameters was explained by bulk soil properties. Higher co-occurrence network complexity and stability emerged in the rhizosphere microbiomes of fine roots than those of coarse roots, in contrast to the situation in the endosphere microbiomes. In particular, the endosphere of roots with a diameter of 1-2 mm exhibited the lowest network complexity and stability and a high proportion of keystone taxa (e.g., Cytophagia, Flavobacteriia, Sphingobacteriia, β-Proteobacteria, and γ-Proteobacteria), suggesting a keystone taxon-reliant strategy in this transitional stage. In summary, this study indicated that root diameter at different root depths differentially affects rhizospheric and endophytic bacterial communities, which implies a close relationship between the bacterial microbiome, root function, and soil properties.
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http://dx.doi.org/10.1007/s00248-020-01678-4DOI Listing
January 2021

Nuclear resonance fluorescence drug inspection.

Sci Rep 2021 Jan 14;11(1):1306. Epub 2021 Jan 14.

School of Nuclear Science and Technology, University of South China, Hengyang, 421001, China.

There is an increasing challenge to prevent illicit drug smuggling across borders and seaports. However, the existing techniques in-and-of-themselves are not sufficient to identify the illicit drugs rapidly and accurately. In the present study, combining nuclear resonance fluorescence (NRF) spectroscopy and the element (or isotope) ratio approach, we present a novel inspection method that can simultaneously reveal the elemental (or isotopic) composition of the illicit drugs, such as widely abused methamphetamine, cocaine, heroin, ketamine and morphine. In the NRF spectroscopy, the nuclei are excited by the induced photon beam, and measurement of the characteristic energies of the emitted [Formula: see text] rays from the distinct energy levels in the excited nuclei provides "fingerprints" of the interested elements in the illicit drugs. The element ratio approach is further used to identify drug elemental composition in principle. Monte Carlo simulations show that four NRF peaks from the nuclei [Formula: see text]C, [Formula: see text]N and [Formula: see text]O can be detected with high significance of 7-24[Formula: see text] using an induced photon beam flux of [Formula: see text]. The ratio of [Formula: see text]/[Formula: see text] and/or [Formula: see text]/[Formula: see text] for illicit drugs inspected are then extracted using the element ratio approach. It is found that the present results of simulations are in good agreement with the theoretical calculations. The feasibility to detect the illicit drugs, inside the 15-mm-thick iron shielding, or surrounded by thin benign materials, is also discussed. It is indicated that, using the state-of-the-art [Formula: see text]-ray source of high intensity and energy-tunability, the proposed method has a great potential for identifying drugs and explosives in a realistic measurement time.
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http://dx.doi.org/10.1038/s41598-020-80079-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809066PMC
January 2021

Crude glycerol impurities improve lipase production by .

Prep Biochem Biotechnol 2021 Jan 13:1-11. Epub 2021 Jan 13.

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, People's Republic of China.

Crude glycerol, a by-product of biodiesel production, was employed as the carbon source to produce lipase using . Under identical fermentation conditions, cell growth and lipase activity were improved using crude glycerol instead of pure glycerol. The impacts of crude glycerol impurities (methyl ester, grease, glycerol, methanol, and metal ions Na, Ca, and Fe) on lipase production were investigated. Impurities accelerated entering the stationary phase. Na, Ca, and grease in waste crude glycerol were the main factors influencing higher lipase activity. Through response surface optimization of Ca, Na, and grease concentrations, lipase activity reached 1437 U/mL (15,977 U/mg), which was 2.5 times that of the control. This study highlights the economical and highly efficient valorization of crude glycerol, demonstrating its possible utilization as a carbon source to produce lipase by without pretreatment.
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http://dx.doi.org/10.1080/10826068.2020.1870135DOI Listing
January 2021

Effects of continuous positive airway pressure on plasma fibrinogen levels in obstructive sleep apnea patients: a systemic review and meta-analysis.

Biosci Rep 2021 Jan;41(1)

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Objective: Fibrinogen has been implicated to play a role in the pathophysiology of obstructive sleep apnea (OSA). Many studies have evaluated the effect of continuous positive airway pressure (CPAP) on plasma fibrinogen levels in OSA patients. However, results from different reports were not consistent. To assess the effect of CPAP treatment on plasma fibrinogen levels of patients with OSA, a meta-analysis was performed.

Methods: A systematic search of Pubmed, Embase, Cochrane, Wanfang Database and Chinese National Knowledge Infrastructure was performed. Data were extracted, and then weighted mean difference (WMD) and 95% confidence intervals (CIs) were calculated using a random-effects model.

Results: Twenty-two studies involving 859 patients were included in this meta-analysis. Combined data showed that plasma fibrinogen concentrations decreased after CPAP therapy (WMD = -0.38 g/l, 95% CI [-0.54 to -0.22 g/l], P<0.001). In the subgroup analyses by therapy duration, plasma fibrinogen concentrations declined significantly in the long-term (≥1 month) CPAP therapy subgroup (WMD = -0.33 g/l, 95% CI [-0.49 to -0.16 g/l], P<0.001) but not in the short-term (<1 month) CPAP therapy subgroup (WMD = -0.84 g/l, 95% CI [-1.70 to 0.03 g/l], P=0.058). Moreover, in patients with long-term CPAP therapy duration, plasma fibrinogen levels decreased with good CPAP compliance (≥4 h/night) (WMD = -0.37 g/l, 95% CI [-0.55 to -0.19 g/l], P<0.001) but not with poor CPAP compliance (<4 h/night) (WMD = 0.12 g/l, 95% CI [-0.09 to 0.33 g/l], P=0.247).

Conclusion: Long-term CPAP treatment with good compliance can reduce the plasma fibrinogen levels in patients with OSA.
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http://dx.doi.org/10.1042/BSR20203856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846965PMC
January 2021

miR-429-3p/ Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway.

Front Cell Dev Biol 2020 21;8:595637. Epub 2020 Dec 21.

Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China.

To explore the regulatory mechanism of abdominal fat deposition in broilers, 100-day-old Sanhuang chickens ( = 12) were divided into high-fat and low-fat groups, according to the abdominal fat ratio size. Total RNA isolated from the 12 abdominal fat tissues was used for miRNA and mRNA sequencing. Results of miRNA and mRNA sequencing revealed that miR-429-3p was highly expressed in high-fat chicken whereas expression was downregulated. Further, we determined that miR-429-3p promoted preadipocyte proliferation and differentiation, whereas exerted an opposite effect. Notably, we found that the miR-429-3p/ axis facilitated PPARγ pathway activation, which is closely associated with the progression of adipogenesis. In conclusion, our results provide evidence that a novel miR-429-3p/ axis is involved in the regulation of adipogenesis, which may have a guiding role in the improvement of breeding for abdominal fat traits in broiler chickens.
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http://dx.doi.org/10.3389/fcell.2020.595637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793751PMC
December 2020