Publications by authors named "Wen Li"

3,471 Publications

  • Page 1 of 1

MECOM promotes supporting cell proliferation and differentiation in cochlea.

J Otol 2022 Apr 24;17(2):59-66. Epub 2021 Nov 24.

Department of Otolaryngology-Head and Neck Surgery of Yijishan Hospital,Wannan Medical College, Wuhu, China.

Permanent damage to hair cells (HCs) is the leading cause of sensory deafness. Supporting cells (SCs) are essential in the restoration of hearing in mammals because they can proliferate and differentiate to HCs. MDS1 and EVI1 complex locus is vital in early development and cell differentiation and regulates the TGF-β signaling pathway to adapt to pathophysiological events, such as hematopoietic proliferation, differentiation and cells death. In addition, plays an essential role in neurogenesis and craniofacial development. However, the role of in the development of cochlea and its way to regulate related signaling are not fully understood. To address this problem, this study examined the expression of MECOM during the development of cochlea and observed a significant increase of MECOM at the key point of auditory epithelial morphogenesis, indicating that may have a vital function in the formation of cochlea and regeneration of HCs. Meanwhile, we tried to explore the possible effect and potential mechanism of in SC proliferation and HC regeneration. Findings from this study indicate that overexpression of MECOM markedly increases the proliferation of SCs in the inner ear, and the expression of Smad3 and Cdkn2b related to TGF signaling is significantly down-regulated, corresponding to the overexpression of MECOM. Collectively, these data may provide an explanation of the vital function of in SC proliferation and trans-differentiation into HCs, as well as its regulation. The interaction between , Wnt, Notch and the TGF-β signaling may provide a feasible approach to induce the regeneration of HCs.
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http://dx.doi.org/10.1016/j.joto.2021.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349018PMC
April 2022

Upgrade the high-load anaerobic digestion and relieve acid stress through the strategy of side-stream micro-aeration: biochemical performances, microbial response and intrinsic mechanisms.

Water Res 2022 Aug 11;221:118850. Epub 2022 Jul 11.

School of Architecture and Urban Planning, Chongqing Jiaotong University, Chongqing 400074, PR China.

In high-load anaerobic digestion such as in kitchen waste, side-stream micro-aeration (SMA) shows excellent operational performance to direct micro-aeration (DMA). It immediately restores the acidification to stability. Methanogenic performance remained stable when organic load ratios (OLR) was further increased to 5.5 g VS/L. Enhanced enzyme activity, microbial aggregation, and proliferation of bacteria and archaea were observed in SMA. The results indicates that SMA enriched Methanosaeta (relative abundance exceeded 93%) and induced the change of the main methanogenic pathway to acetoclastic methanogenesis. Mechanisms was further explored by using metagenomic analysis, and the results show SMA avoids mass formation of ROS (reactive oxygen species) by cycling the aerated slurry, and retains benefits of trace O on material and energic metabolism, which poses great application potentials and deserves further investigation.
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http://dx.doi.org/10.1016/j.watres.2022.118850DOI Listing
August 2022

B-to-A transition in target DNA during retroviral integration.

Nucleic Acids Res 2022 Aug 10. Epub 2022 Aug 10.

The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Integration into host target DNA (tDNA), a hallmark of retroviral replication, is mediated by the intasome, a multimer of integrase (IN) assembled on viral DNA (vDNA) ends. To ascertain aspects of tDNA recognition during integration, we have solved the 3.5 Å resolution cryo-EM structure of the mouse mammary tumor virus (MMTV) strand transfer complex (STC) intasome. The tDNA adopts an A-like conformation in the region encompassing the sites of vDNA joining, which exposes the sugar-phosphate backbone for IN-mediated strand transfer. Examination of existing retroviral STC structures revealed conservation of A-form tDNA in the analogous regions of these complexes. Furthermore, analyses of sequence preferences in genomic integration sites selectively targeted by six different retroviruses highlighted consistent propensity for A-philic sequences at the sites of vDNA joining. Our structure additionally revealed several novel MMTV IN-DNA interactions, as well as contacts seen in prior STC structures, including conserved Pro125 and Tyr149 residues interacting with tDNA. In infected cells, Pro125 substitutions impacted the global pattern of MMTV integration without significantly altering local base sequence preferences at vDNA insertion sites. Collectively, these data advance our understanding of retroviral intasome structure and function, as well as factors that influence patterns of vDNA integration in genomic DNA.
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http://dx.doi.org/10.1093/nar/gkac644DOI Listing
August 2022

Circ_0001058 represses the progression of lung adenocarcinoma through governing of the miR-486-5p/TEK signaling axis.

Anticancer Drugs 2022 Aug 9. Epub 2022 Aug 9.

Department of Respiratory and Critical Care Medicine, Chengdu First People's Hospital, Chengdu, Sichuan, China.

The most common type of lung cancer is lung adenocarcinoma. Emerging views believe that circular RNA (circRNA) participates in its pathogenesis. The objective of this study is to find out the potential functions and mechanisms of circ_0001058 in lung adenocarcinoma pathogenesis. To detect circ_0001058, miR-486-5p and TEK tyrosine kinase (TEK) receptor tyrosine kinase expressions, real-time quantitative PCR (RT-qPCR) and western blotting were performed. Cell functions, including proliferation, apoptosis and invasion, were then evaluated using cell counting kit-8, caspase-3 activity and transwell assays, respectively. To establish the role of circ_0001058 in tumorigenesis, nude mice were utilized as in-vivo models. The predicted binding relationships of miR-486-5p to circ_0001058 or TEK were further verified by performing a dual-luciferase assay and ribonucleoprotein immunoprecipitation (RIP) analysis. Decreased circ_0001058 expression was observed in lung adenocarcinoma cells and tissue specimens. Circ_0001058 was predominantly situated in the cytoplasm and was greatly resistant to RNase R digestion. Circ_0001058 overexpression restrained A549 and PC9 cells' abilities to proliferate, survive and invade, and it also repressed tumorigenesis in the animal models. Circ_0001058 directly targeted miR-486-5p and depleted its expression. Restoring miR-486-5p could invert the inhibitory effects of circ_0001058 in the cancer cell phenotypes. Furthermore, miR-486-5p targeted TEK, so the inhibitory effects of TEK overexpression on the malignant behaviors of A549 and PC9 cells could also be abolished by miR-486-5p restoration. Circ_0001058 overexpression blocked the malignant development of lung adenocarcinoma via modulation of the miR-486-5p/TEK pathway. These results contribute new insights on the pathogenesis of lung adenocarcinoma.
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http://dx.doi.org/10.1097/CAD.0000000000001337DOI Listing
August 2022

Melatonin maintained higher contents of unsaturated fatty acid and cell membrane structure integrity in banana peel and alleviated postharvest chilling injury.

Food Chem 2022 Aug 1;397:133836. Epub 2022 Aug 1.

School of Food Science and Engineering, Hainan University, Haikou 570228, China. Electronic address:

Exogenous melatonin confers the chilling tolerance of banana fruit by promoting reactive oxygen species (ROS) scavenging system and inducing the unsaturated fatty acid synthesis. The results showed that melatonin treatment increased the contents of phospholipids, promoted the ROS scavenging enzyme, and restrained the activities of lipoxygenase (LOX), and thus reduced the lipid peroxidation of banana peel. In addition, melatonin treatment increased the flavonoids and proline contents, which was conducive to antioxidant capacity. Interestingly, the enhanced antioxidant capacity is conducive to the stability of unsaturated fatty acids and reduce the enzymatic browning reaction. Moreover, melatonin treatment induced the expression of omega-3/6 fatty acid desaturase and triggered the fatty acid metabolism activity, by which maintained higher contents of unsaturated fatty acid in banana peel. Moreover, melatonin treatment stimulated the accumulation of fatty acids in banana peel, and was involved in alleviating fruit chilling injury.
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http://dx.doi.org/10.1016/j.foodchem.2022.133836DOI Listing
August 2022

Comparison of the diagnostic performance of shear wave elastography with shear wave dispersion for pre-operative staging of hepatic fibrosis in patients with hepatocellular carcinoma.

Eur J Radiol 2022 Jul 29;154:110459. Epub 2022 Jul 29.

Department of Ultrasound, Yancheng First Hospital, Affiliated Hospital of Nanjing University Medical School, 166 Yulong Road, Tinghu District, Yancheng 224000, China; Department of Ultrasound, The First people's Hospital of Yancheng, 166 Yulong Road, Tinghu District, Yancheng 224000, China. Electronic address:

Purpose: This prospective study aimed to compare the diagnostic accuracy of shear wave elastography (SWE) with that of shear wave dispersion (SWD) in evaluation of hepatic fibrosis in patients with hepatocellular carcinoma before resection.

Method: A total of 210 consecutive patients with hepatocellular carcinoma (HCC) who were scheduled to undergo hepatectomy were prospectively enrolled, pre-operative SWE and SWD examinations were performed. Fibrosis staging and necroinflammatory activity were determined histopathologically according to the Scheuer standard. Multivariate linear regression analysis was used to identify factors associated with SWE and SWD values. The performance of SWE and SWD were determined by receiver operating characteristic (ROC) analysis.

Results: Both SWE and SWD values of liver were highly correlated with liver fibrosis stage and necroinflammatory activity (p < 0.05). Both SWE and SWD values were significantly different among the patients with different stages of liver fibrosis (p < 0.001). ROC analysis revealed that SWE that predicted substantial fibrosis (S ≥ 2), severe fibrosis (S ≥ 3) and cirrhosis (S = 4) were 0.895, 0.877 and 0.854, the SWD that predicted substantial fibrosis (S ≥ 2), severe fibrosis (S ≥ 3) and cirrhosis (S = 4) were 0.857, 0.815 and 0.791. SWE had better diagnostic performance than SWD in predicting severe fibrosis (S ≥ 3) and cirrhosis (S = 4).

Conclusions: Both SWE and SWD are useful, accurate and non-invasive methods for evaluating hepatic fibrosis in patients with hepatocellular carcinoma adapted to hepatectomy, SWE is a more accurate imaging modality than SWD in predicting severe fibrosis (S ≥ 3) and cirrhosis (S = 4).
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http://dx.doi.org/10.1016/j.ejrad.2022.110459DOI Listing
July 2022

Colloidal Jamming by Interfacial Self-Assembled Polymers: A Robust Route for Ultrahigh Efficient Encapsulation.

Angew Chem Int Ed Engl 2022 Aug 4. Epub 2022 Aug 4.

China Pharmaceutical University, School of Pharmacy, Longmian Avenue No. 639, 211198, Nanjing, CHINA.

The control of cargo phase-transfer is of interest for many applications in science and technology. Here, we report a simple, versatile and robust method to block the phase-transfer of cargo colloids by interfacial self-assembled amphiphilic polymer molecules. After simply increasing the concentration of amphiphilic polymers, the orientation of interfacial polymer molecules changed from flat to upright direction, forming a thick three-dimensional polymer layer at the oil-water surface. Even under fierce external force, this thick interfacial layer robustly jammed the phase-transfer of cargo colloids, resulting in an ultrahigh encapsulation efficiency (up to 97.1%) for proteins and peptides. One single injection of high insulin-loaded microcomposites (58.3 wt%) persisted the blood glucose level within normoglycemic state for 10 days in type 1 diabetic rats. The mass of administrated amphiphilic polymers was 1889 times smaller than that of microcomposites prepared with non-amphiphilic ones.
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http://dx.doi.org/10.1002/anie.202208738DOI Listing
August 2022

Lysine stimulates the development of the murine mammary gland at puberty via PI3K/AKT/mTOR signalling axis.

J Anim Physiol Anim Nutr (Berl) 2022 Aug 3. Epub 2022 Aug 3.

Department of Theoretic Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.

Lysine is one of the essential amino acids. The effect of lysine on milk protein and milk fat anabolism has been reported, but the effect on mammary glands development has not been studied in detail. The normal development of the mammary glands at puberty is crucial to lactation of mammals. In this study, to explore the effect of lysine on mammary glands development, we fed different concentrations of lysine (0.025%, 0.05%, 0.1%) to pubertal mice and found that the addition of 0.1% lysine to drinking water significantly promoted mammary glands development. Furthermore, we treated mMECs (mouse mammary epithelial cells) with different concentrations of lysine (0, 0.2, 0.4, 0.6, 0.8 and 1 mM) to explore the underlying mechanism, and found that lysine promoted the proliferation of mMECs and development of mammary glands through PI3K/AKT/mTOR signalling pathway in pubertal mice. Overall, the results of this study revealed that lysine activated the PI3K/AKT/mTOR signal axis, elevated protein concentrations of cell proliferation markers, such as PCNA, Cyclin D1 and D3, and enhanced the proliferation of mMECs, finally promoted the murine mammary glands development at puberty.
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http://dx.doi.org/10.1111/jpn.13756DOI Listing
August 2022

Transcription Factor CpbHLH3 and Gene Cooperatively Regulate Fruit Texture and Counteract 1-Methylcyclopropene Inhibition of Softening in Postharvest Papaya ( L.).

J Agric Food Chem 2022 Aug 3. Epub 2022 Aug 3.

Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou 570228, P. R. China.

Papaya ( L.) is a climacteric fruit susceptible to postharvest losses attributable to ethylene-induced ripening and softening. In this study, we examined the effect of 1-methylcyclopropene (1-MCP) treatment (1 μL L for 20 h) on the textural properties of "SunUp" papaya fruit and investigated the regulatory mechanisms of molecular profiles. Compared with control, postharvest 1-MCP treatment significantly inhibited fruit softening, which is associated with higher hemicellulose content and lower xylanase activity of papaya fruit. Moreover, RNA-seq and qRT-PCR analyses indicated that and were differentially expressed during storage. Yeast one-hybrid, electrophoretic mobility shift assays, and dual-luciferase reporter assays disclosed that CpbHLH3 activated the transcription of by binding directly to its promoter. Transient overexpression of alleviates the inhibitory effect of 1-MCP on softening by increasing xylanase activity and upregulating the gene expression. Our observations provide new insights into the transcriptional regulatory mechanisms that govern softening of postharvest papaya fruit.
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http://dx.doi.org/10.1021/acs.jafc.2c01908DOI Listing
August 2022

Alterations in soil microbial phospholipid fatty acid profile with soil depth following cropland conversion in karst region, southwest China.

Environ Sci Pollut Res Int 2022 Aug 2. Epub 2022 Aug 2.

Hunan Soil and Fertilizer Institute, Changsha, 410125, Hunan, China.

The soil microbial community is a key indicator used to evaluate the effectiveness of land use changes in degraded ecosystems. However, there is currently limited information on the vertical variations of soil microbial communities under different cropland types in the karst region of southwest China. In this study, three different cropland types (sugarcane, mulberry, and forage grass) were selected to replace maize-soybean cultivation in a karst area of southwest China. Secondary forest was included for comparison. Soil was collected at soil depths of 0-10 cm, 10-20 cm, 20-30 cm, and 30-50 cm to conduct a phospholipid fatty acid (PLFA) analysis of the soil microbial communities. The results showed that the abundances of total PLFAs and PLFAs of bacteria, fungi, actinomycetes, and arbuscular mycorrhizal fungi were significantly higher in forage grass cultivation and secondary forest areas than those in other three cropland types. The abundance of total PLFA and its functional groups significantly decreased with increasing soil depth. Bacteria was the predominant community (> 52%) across all land use types and soil depths. A principal component analysis indicated that soil microbial PLFA composition in forage grass field and secondary forest areas differed significantly from that in mulberry, sugarcane, and maize-soybean fields across 0-50-cm soil layer. Soil organic carbon was identified as the primary factor affecting the abundance and structure of soil microbial communities in the four soil layers. These results suggest that greater improvements in soil quality and fertility could be made by replacing the maize-soybean rotation system with forage grass cultivation than by replacing it with mulberry or sugarcane cultivation in the karst region of southwest China.
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http://dx.doi.org/10.1007/s11356-022-22178-7DOI Listing
August 2022

Lobe-specific lymph node dissection in early-stage non-small cell lung cancer: An overview.

Asian J Surg 2022 Jul 30. Epub 2022 Jul 30.

Department of Thoracic Surgery, West China Hospital of Sichuan University, Chengdu, China. Electronic address:

Lymph node dissection is a vital part of surgical treatment for early-stage non-small cell lung cancer (NSCLC). Lobectomy with systematic lymph node dissection (SLND) still remains the gold standard surgical treatment for early-stage NSCLC patients. However, an increasing number of studies have demonstrated that lobe-specific lymph node dissection (L-SLND) can be used as an alternative therapy for SLND in carefully selected patients with early-stage NSCLC. However, there are no currently available evidences of review summarizing the role of L-SLDN in treating early-stage NSCLC. Therefore, we performed this literature review by summarizing the existing literatures on the lymph node drainage pattern, definition, scope and role of L-SLND in patients with early-stage NSCLC, aiming to provide evidence for the application of L-SLND in patients with early-stage NSCLC.
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http://dx.doi.org/10.1016/j.asjsur.2022.07.042DOI Listing
July 2022

An update on biomaterials as microneedle matrixes for biomedical applications.

J Mater Chem B 2022 Aug 2. Epub 2022 Aug 2.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 10029, China.

Microneedles (MNs) have been developed for various applications such as drug delivery, cosmetics, diagnosis, and biosensing. To meet the requirements of MNs used in these areas, numerous materials have been used for the fabrication of MNs. However, MNs will be exposed to skin tissues after piercing the stratum corneum barrier. Thus, it is necessary to ensure that the matrix materials of MNs have the characteristics of low toxicity, good biocompatibility, biodegradability, and sufficient mechanical properties for clinical application. In this review, the matrix materials currently used for preparing MNs are summarized and reviewed in terms of these factors. In addition, MN products used on the market and their applications are summarized in the end. This work may provide some basic information to researchers in the selection of MN matrix materials and in developing new materials.
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http://dx.doi.org/10.1039/d2tb00905fDOI Listing
August 2022

Multiple Sevoflurane Exposures During the Neonatal Period Cause Hearing Impairment and Loss of Hair Cell Ribbon Synapses in Adult Mice.

Front Neurosci 2022 14;16:945277. Epub 2022 Jul 14.

Department of Anesthesiology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Objectives: This study aims to investigate the effects of multiple sevoflurane exposures in neonatal mice on hearing function in the later life and explores the underlying mechanisms and protective strategies.

Materials And Methods: Neonatal Kunming mice were exposed to sevoflurane for 3 days. Auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) tests, immunofluorescence, patch-clamp recording, and quantitative real-time PCR were performed to observe hearing function, hair cells, ribbon synapses, nerve fibers, spiral ganglion neurons, and oxidative stress.

Results: Compared to control group, multiple sevoflurane exposures during the neonatal time significantly elevated ABR thresholds at 8 kHz (35.42 ± 1.57 vs. 41.76 ± 1.97 dB, = 0.0256), 16 kHz (23.33 ± 1.28 vs. 33.53 ± 2.523 dB, = 0.0012), 24 kHz (30.00 ± 2.04 vs. 46.76 ± 3.93 dB, = 0.0024), and 32 kHz (41.25 ± 2.31 vs. 54.41 ± 2.94 dB, = 0.0028) on P30, caused ribbon synapse loss on P15 (13.10 ± 0.43 vs. 10.78 ± 0.52, = 0.0039) and P30 (11.24 ± 0.56 vs. 8.50 ± 0.84, = 0.0141), and degenerated spiral ganglion neuron (SGN) nerve fibers on P30 (110.40 ± 16.23 vs. 55.04 ± 8.13, = 0.0073). In addition, the V of calcium current become more negative (-21.99 ± 0.70 vs. -27.17 ± 0.60 mV, < 0.0001), exocytosis was reduced (105.40 ± 19.97 vs. 59.79 ± 10.60 fF, < 0.0001), and was upregulated ( = 0.0219) in sevoflurane group than those in control group. -acetylcysteine (NAC) reversed hearing impairment induced by sevoflurane.

Conclusion: The findings suggest that multiple sevoflurane exposures during neonatal time may cause hearing impairment in adult mice. The study also demonstrated that elevated oxidative stress led to ribbon synapses impairment and SGN nerve fibers degeneration, and the interventions of antioxidants alleviated the sevoflurane-induced hearing impairment.
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http://dx.doi.org/10.3389/fnins.2022.945277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329801PMC
July 2022

Automatic Schizophrenia Detection Using Multimodality Media via a Text Reading Task.

Front Neurosci 2022 14;16:933049. Epub 2022 Jul 14.

College of Biomedical Engineering, Sichuan University, Chengdu, China.

Schizophrenia is a crippling chronic mental disease that affects people worldwide. In this work, an automatic schizophrenia detection algorithm is proposed based on the reading deficit of schizophrenic patients. From speech and video modalities, the automatic schizophrenia detection algorithm illustrates abnormal speech, head movement, and reading fluency during the reading task. In the speech modality, an acoustic model of speech emotional flatness in schizophrenia is established to reflect the emotional expression flatness of schizophrenic speech from the perspective of speech production and perception. In the video modality, the head-movement-related features are proposed to illustrate the spontaneous head movement caused by repeated reading and unconscious movement, and the reading-fluency-related features are proposed to convey the damaged degree of schizophrenic patients' reading fluency. The experimental data of this work are 160 segments of speech and video data recorded by 40 participants (20 schizophrenic patients and 20 normal controls). Combined with support vector machines and random forest, the accuracy of the proposed acoustic model, the head-movement-related features, and the reading-fluency-related features range from 94.38 to 96.50%, 73.38 to 83.38%, and 79.50 to 83.63%, respectively. The average accuracy of the proposed automatic schizophrenia detection algorithm reaches 97.50%. The experimental results indicate the effectiveness of the proposed automatic detection algorithm as an auxiliary diagnostic method for schizophrenia.
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http://dx.doi.org/10.3389/fnins.2022.933049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331283PMC
July 2022

Study on freezing characteristics of the surface water film over glaze ice by using an ultrasonic pulse-echo technique.

Ultrasonics 2022 Jul 14;126:106804. Epub 2022 Jul 14.

Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China; Nanjing University of Aeronautics and Astronautics State Key Laboratory of Mechanics and Control of Mechanical Structures, Nanjing 210016, China. Electronic address:

This study proposes an ultrasonic pulse-echo technique to examine the freezing characteristics of a thin film of water over ice, and uses it to develop a method to measure the thickness of glaze ice. A multilayer model is first introduced to simulate ultrasonic transmission through multiple media. A transition layer is then inserted between the layers of ice and water, and its properties were in gradient form along the direction of thickness. Following this, a high-frequency ultrasonic experimental device is developed to dynamically measure variations in the thickness of the layers of ice and water. The accuracy of the proposed model of the transition layer was validated by showing that its numerical results agreed well with those of experiments. The results show that the amplitude of echo from the top of the ice layer was at its minimum when the thickness of the film of water was in the range of [40, 45] μm, and increased when the film of water was thinner than 40 μm. A delay in echo from the top of the layer of ice was observed when measuring its thickness because the film of water froze, which yielded a relative error of 3.34%. The proposed numerical model can thus efficiently measure the thickness of glaze ice.
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http://dx.doi.org/10.1016/j.ultras.2022.106804DOI Listing
July 2022

Exploration of long afterglow luminescent materials composited with graphitized carbon nitride for photocatalytic degradation of basic fuchsin.

Environ Sci Pollut Res Int 2022 Jul 28. Epub 2022 Jul 28.

School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan, China.

The frequent exposure of the widely used dye, basic fuchsin (BF), is seriously threatening the health of human central nervous system. Thus, removing the environmental pollution caused by BF is crucial, and photocatalytic technology recently has been used to degrade the pollutions dye. In this study, the binary composite SrAlO:Eu, Dy/g-CN was prepared by high-temperature calcination and then applied in BF photodegradation. The results confirmed that the composite material had lower band gap value (Eg) and stronger visible light absorption ability. The photocatalytic capacity of the new composite materials was enhanced compared to that of the non-composite materials. By using the new binary-composited materials, 80% of BF could be degraded in 10 min, and the degradation ratio reached 100% in 30 min. More importantly, even the light source was removed, the photocatalytic reaction could continue due to the luminescence of SrAlO:Eu, Dy, and the degradation efficiency of BF could finally reach more than 90% within 3 h. By quenching experiments and electron spin resonance (ESR) spectra analysis, superoxide anion (·O) was verified to be the main active substance in this reaction process. Moreover, the excellent stability and recyclability of this catalyst was also proved. Furthermore, the new composite materials were utilized to degrade the BF aqueous solution and actual lake water, and the total organic matter contents (TOC) were measured. TOC values in these two systems decreased after photocatalytic reaction, which indicated that this catalyst has a great development prospect in the removal of organic matter in water. Our study confirmed a new kind of material of high performance with great significance for emergency treatment of water pollution in practical applications.
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http://dx.doi.org/10.1007/s11356-022-22097-7DOI Listing
July 2022

Prevalence and burden of chronic cough in China: a national cross-sectional study.

ERJ Open Res 2022 Jul 25;8(3). Epub 2022 Jul 25.

National Center of Gerontology, Beijing Hospital, Beijing, China.

Background: Chronic cough is a common complaint, but there are no population-based data on its burden in China. We determined the prevalence of chronic cough and its impact on health status in adults stratified by sex, age and the diagnosis of COPD or the presence of small airway dysfunction (SAD).

Methods: A representative sample of 57 779 Chinese adults aged 20 years or older was recruited and pulmonary function test was measured. Chronic cough was defined as cough lasting for >3 months in each year. Quality of life was assessed by the 12-item Short Form Health Survey (SF-12), and self-reported history of hospital visits was recorded.

Results: Chronic cough was found in 3.6% (95% CI 3.1-4.1) of Chinese adults, 2.4% (95% CI 1.9-3.1) of those aged 20-49 years and 6.0% (95% CI 5.3-6.8) of those aged 50 years or older. Individuals with chronic cough had an impaired physical component summary (PCS) score of the SF-12 (p<0.0001) and more emergency visits (p=0.0042) and hospital admissions (p=0.0002). Furthermore, the impact of chronic cough on PCS score was more significant in those aged 50 years or older, or with COPD (p0.0018 or 0.0002, respectively), with the impact on hospital admission being more significant in those with COPD or with SAD (p=0.0026 or 0.0065, respectively).

Conclusions: Chronic cough is prevalent in China and is associated with a poorer health status, especially in individuals aged 50 years or older and those with the diagnosis of COPD or SAD.
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http://dx.doi.org/10.1183/23120541.00075-2022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309344PMC
July 2022

Differences in Humoral Immune Response against the Type 2 Porcine Reproductive and Respiratory Syndrome Virus via Different Immune Pathways.

Viruses 2022 06 29;14(7). Epub 2022 Jun 29.

College of Veterinary Medicine, Henan Agricultural University, Zhengdong New District Longzi Lake 15#, Zhengzhou 450046, China.

The intramuscular vaccine is the principal strategy to protect pigs from porcine reproductive and respiratory syndrome virus (PRRSV), However, it is still difficult to control PRRSV effectively. This study infected piglets with PRRSV through intramuscular and intranasal inoculation. Subsequently, viral loads, anti-PRRSV antibody levels, and neutralizing antibodies (NAs) titers in both serum and saliva were monitored for 43 days. Meanwhile, tissues were obtained through necropsy at 43 days post-inoculation (dpi) to detect viral loads. The results indicated that viremia lasted from 3 to 31 dpi in both the inoculation groups, but the viruses survived in the lungs and lymph nodes after viremia clearance. The antibody response was detected from 11 dpi, but the response of NAs was delayed until 3-4 weeks. Furthermore, intranasal inoculation induced lower viral load levels than injection inoculation. In addition, positive SIgA and NAs levels were produced early, with higher levels through intranasal inoculation. Therefore, our data indicated that a more robust antibody response and lower virus loads could be induced by intranasal inoculation, and mucosal inoculation could be a suitable pathway for PRRSV vaccines.
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http://dx.doi.org/10.3390/v14071435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316826PMC
June 2022

Signal Inversion and Amplification of Circularly Polarized Luminescence in a Poly(phenylacetylene)-Based Composite System Assisted by Achiral PMMA.

ACS Appl Mater Interfaces 2022 Aug 26;14(31):36157-36165. Epub 2022 Jul 26.

CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei 230026, Anhui, P. R. China.

Multichannel regulable circularly polarized luminescence (CPL) is fascinating because of its fundamental and application interest. There are few reports on helical sense (-/-helix) modulation and chiral signal amplification of polyacetylenes with the assistance of achiral polymers and further applications in precisely and conveniently regulating CPL handedness and magnitude. Herein, a helical poly(phenylacetylene)-based CPL-active system was constructed, in which CPL inversion occurred by adding achiral PMMA into a helical poly(phenylacetylene)-CHCl solution. Significantly, there is almost 10 times magnification of luminescence dissymmetry factor values (||) during this process. The above phenomena could be ascribed to the PMMA-assisted polyene backbone elongation and the formation of a more ordered helical structure for the poly(phenylacetylene)s. More interestingly, the CPL signal can be facilely inverted and switched by simply changing the thickness of the PPhAD/PMMA layer. The temperature-driven dynamic CPL handedness inversion and magnitude modulation can also be achieved. Based on the multiple regulations for CPL, logic operations were developed, and the practical application is further facilitated by designing various CPL patterns. This study establishes effective and convenient strategies to switch the handedness, magnitude, and wavelength of CPL, which may generate a breakthrough in the manufacturing of CPL-active smart materials and devices with promising application potential.
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http://dx.doi.org/10.1021/acsami.2c09861DOI Listing
August 2022

The safety and efficacy of EUS-GE for gastric outlet obstruction in different sites: a single-center retrospective study.

J Dig Dis 2022 Jul 26. Epub 2022 Jul 26.

Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School.

Background: Gastric outlet obstruction (GOO) is a common indication for palliative surgical consultation. Recently, EUS-guided gastroenterostomy (EUS-GE) has become a novel option. The aim of this study is to compare the safety and efficacy of EUS-GE in different obstruction positions.

Methods: Consecutive patients, who underwent EUS-GE for GOO from March 2017 to April 2020 in our center and stayed in gastroenterology ward after the operation, were involved in this retrospective study. Patients were divided into three groups depending on obstruction site. The primary outcomes included technical success and clinical success. The secondary outcomes were operation time, post-procedure length of stay (LOS), hospitalization costs and complications, including peritonitis, bleeding, pneumoperitoneum, abdominal pain and infection.

Results: A total of 51 patients were included. Technical success rate was 100% in the proximal position, while 88.9% in the distal position (P = 0.176). Clinical success declined from the oral side to the anal side (P = 0.510). Mean operation time, hospitalization costs and post-operation LOS were similar (P = 0.532, 0.520, and 0.144, respectively). Complications were observed in 28 patients (54.9%). When reaching mature phase, clinical success rate enhanced and the secondary outcomes showed no significant difference.

Conclusions: EUS-GE may be challenging for distal GOO, however it was safe and effective to be conducted by experienced endoscopists. Taking complete preoperative evaluation to evaluate the difficulty degree is necessary. Larger and prospective data are needed to further validate our finding.
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http://dx.doi.org/10.1111/1751-2980.13118DOI Listing
July 2022

Characterization of Warmed-Over Flavor Compounds in Surimi Gel Made from Silver Carp () by Gas Chromatography-Ion Mobility Spectrometry, Aroma Extract Dilution Analysis, Aroma Recombination, and Omission Studies.

J Agric Food Chem 2022 Aug 25;70(30):9451-9462. Epub 2022 Jul 25.

College of Food Science and Technology/National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Huazhong Agricultural University, Wuhan, Hubei Province 430070, P. R. China.

The warmed-over flavor (WOF) in surimi gels was characterized by gas chromatography-ion mobility spectrometry, aroma extract dilution analysis, aroma recombination, and omission studies. Surimi gels with different WOF levels were prepared by different gelling temperatures, and surimi gels heated at 90, 100, and 121 °C were considered as the samples with light, strong, and medium WOF, respectively. Based on the quantification and odor activity values, 14 aldehydes, 2 ketones, 3 alcohols, 2 benzene-containing compounds, 2 N-containing compounds, 3 S-containing compounds, 3 lactones, undecanoic acid, and 4-methylphenol were recombined to build a spiked model for surimi gels with the strongest WOF, which showed the highest similarity with the original sample. Finally, a triangle test involving omission of the aroma compounds from the spiked model proved that the WOF in surimi gels was attributed to (,)-2,4-decadienal, heptanal, octanal, nonanal, decanal, ()-2-nonenal, ()-2-octenal, ()-2-decenal, (,)-2,4-heptadienal, 2,3-pentanedione, 2,6-dimethylpyrazine, 2-propylpyridine, benzothiazole, 2-methoxybenzenethiol, and 2-furfurylthiol.
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http://dx.doi.org/10.1021/acs.jafc.2c02119DOI Listing
August 2022

Reconstruction of an orbital defect in rabbits using a silk fibroin-bone microparticle complex.

Int J Artif Organs 2022 Jul 25:3913988221113354. Epub 2022 Jul 25.

Department of Otology and Skull Base Surgery, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.

Objective: To construct a silk fibroin-bone microparticle composite based on a porous silk fibroin membrane and to study its feasibility as a material to reconstruct an orbital bone defect.

Methods: A 3D porous silk fibroin membrane scaffold was constructed with a defined pore size and incorporated with bone microparticles from a New Zealand rabbit orbital bone defect. The silk fibroin-bone microparticle composite was then implanted into the orbital bone defect to promote osteogenesis along the surface of the porous silk fibroin membrane. The feasibility of constructing an ideal orbital defect repair material and the silk fibroin-bone micronucleus complex was evaluated by animal experiments, molecular biology, histomorphology, imaging, raw molecular mechanisms, and the biological behavior of the material in vivo.

Results: The silk fibroin-bone microparticle composite promotes angiogenesis and osteogenesis to repair bone defects in vivo. Moreover, SF (silk fibroin)/BD (bone dust) complex promotes osteogenesis and angiogenesis by activating FGF2 (Fibroblast Growth Factor 2) and SF scaffolds can bind and restore FGF2.

Conclusion: Silk fibroin is biocompatible and the silk fibroin-bone microparticle complex successfully repaired orbital bone defects. Additionally, fibroblast growth factor expression around or within the remaining incompletely degraded silk fibroin materials was observed in vivo.
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http://dx.doi.org/10.1177/03913988221113354DOI Listing
July 2022

Effects of the Ultrasound-Guided Stellate Ganglion Block on Hemodynamics, Stress Response, and Gastrointestinal Function in Postoperative Patients with Colorectal Cancer.

Comput Intell Neurosci 2022 14;2022:2056969. Epub 2022 Jul 14.

People's Hospital of Deyang City, No. 173 Taishan Road, Deyang, Sichuan Province, China.

Objective: The aim of the study was to study the effects of the ultrasound-guided stellate ganglion block on hemodynamics, stressful response, and postoperative gastrointestinal functions in patients with colorectal cancer.

Methods: A total of 100 patients with colorectal cancer hospitalized from January 2021 to December 2021 were selected. After anesthesia induction, the right stellate ganglion block was performed under ultrasound guidance in the research group and the general anesthesia was performed in the control group. The heart rate (HR), mean arterial pressure (MAP), epinephrine, cortisol, self-rating anxiety scale (SAS), Ramsay sedation score (RSS), postoperative bowel sound recovery time, anal exhaust time, and the incidence of gastrointestinal adverse reactions 24 hours after operation were studied pre-and post-24-hour anesthesia induction.

Results: Following 24-hour operation, the HR and MAP values were largely reduced ( < 0.05). Following 24-hour operation, epinephrine and cortisol became obviously higher ( < 0.05). After 24-hour operation, the levels of epinephrine and cortisol in the research group were greatly lower. The score of the SAS in the study cohort was less than that of the controls ( < 0.05). The RSS of the research group was obviously increased ( < 0.05). The recovery time of intestinal sound and the anal exhaust time of the study cohort became remarkably shorter ( < 0.05). The incidence of gastrointestinal adverse reactions 24 hours after operation of the study cohort was much less common ( < 0.05).

Conclusion: The ultrasound-guided stellate ganglion block can reduce the fluctuation of blood circulation during radical resection of colorectal cancer, reduce postoperative gastrointestinal dysfunction and stress reaction, relieve patients' anxiety, and contribute to the recovery of gastrointestinal function.
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http://dx.doi.org/10.1155/2022/2056969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303094PMC
July 2022

Identification of a Novel Functional Non-synonymous Single Nucleotide Polymorphism in Frizzled Class Receptor 6 Gene for Involvement in Depressive Symptoms.

Front Mol Neurosci 2022 7;15:882396. Epub 2022 Jul 7.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Although numerous susceptibility loci for depression have been identified in recent years, their biological function and molecular mechanism remain largely unknown. By using an exome-wide association study for depressive symptoms assessed by the Center for Epidemiological Studies Depression (CES-D) score, we discovered a novel missense single nucleotide polymorphism (SNP), rs61753730 (Q152E), located in the fourth exon of the frizzled class receptor 6 gene (), which is a potential causal variant and is significantly associated with the CES-D score. Computer-based analysis revealed that the protein configuration and stability, as well as the secondary structure of FZD6 differed greatly between the wild-type (WT) and Q152E mutant. We further found that rs61753730 significantly affected the luciferase activity and expression of in an allele-specific way. Finally, we generated -knockin (-KI) mice with rs61753730 mutation using the CRISPR/Cas9 genome editing system and found that these mice presented greater immobility in the forced swimming test, less preference for sucrose in the sucrose preference test, as well as decreased center entries, center time, and distance traveled in the open filed test compared with WT mice after exposed to chronic social defeat stress. These results indicate the involvement of rs61753730 in depression. Taken together, our findings demonstrate that SNP rs61753730 is a novel functional variant and plays an important role in depressive symptoms.
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http://dx.doi.org/10.3389/fnmol.2022.882396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302575PMC
July 2022

A review of the rationale for the testing of the calcineurin inhibitor tacrolimus for post-ERCP pancreatitis prevention.

Pancreatology 2022 Jul 13. Epub 2022 Jul 13.

Division of Pediatric Gastroenterology, Hepatology & Nutrition, Lucille Packard Children's Hospital at Stanford, Stanford, CA, USA. Electronic address:

Endoscopic retrograde cholangiopancreatography (ERCP) is commonly performed for the management of pancreaticobiliary disorders. The most troublesome ERCP-associated adverse event is post-ERCP pancreatitis (PEP), which occurs in up to 15% of all patients undergoing ERCP. A substantial body of preclinical data support a mechanistic rationale for calcineurin inhibitors in preventing PEP. The findings are coupled with recent clinical data suggesting lower rates of PEP in patients who concurrently use the calcineurin inhibitor tacrolimus (e.g., solid organ transplant recipients). In this review, we will firstly summarize data in support of testing the use of tacrolimus for PEP prophylaxis, either in combination with rectal indomethacin or by itself. Secondly, we propose that administering tacrolimus through the rectal route could be favorable for PEP prophylaxis over other routes of administration.
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http://dx.doi.org/10.1016/j.pan.2022.07.003DOI Listing
July 2022

Prostate cancer as a dedifferentiated organ: androgen receptor, cancer stem cells, and cancer stemness.

Essays Biochem 2022 Jul 22. Epub 2022 Jul 22.

Department of Pharmacology & Therapeutics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, U.S.A.

Cancer progression is characterized and driven by gradual loss of a differentiated phenotype and gain of stem cell-like features. In prostate cancer (PCa), androgen receptor (AR) signaling is important for cancer growth, progression, and emergence of therapy resistance. Targeting the AR signaling axis has been, over the decades, the mainstay of PCa therapy. However, AR signaling at the transcription level is reduced in high-grade cancer relative to low-grade PCa and loss of AR expression promotes a stem cell-like phenotype, suggesting that emergence of resistance to AR-targeted therapy may be associated with loss of AR signaling and gain of stemness. In the present mini-review, we first discuss PCa from the perspective of an abnormal organ with increasingly deregulated differentiation, and discuss the role of AR signaling during PCa progression. We then focus on the relationship between prostate cancer stem cells (PCSCs) and AR signaling. We further elaborate on the current methods of using transcriptome-based stemness-enriched signature to evaluate the degree of oncogenic dedifferentiation (cancer stemness) in pan-cancer datasets, and present the clinical significance of scoring transcriptome-based stemness across the spectrum of PCa development. Our discussions highlight the importance to evaluate the dynamic changes in both stem cell-like features (stemness score) and AR signaling activity across the PCa spectrum.
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http://dx.doi.org/10.1042/EBC20220003DOI Listing
July 2022

Effect of CRRT with oXiris filter on hemodynamic instability in surgical septic shock with AKI: A pilot randomized controlled trial.

Int J Artif Organs 2022 Jul 21:3913988221107947. Epub 2022 Jul 21.

Department of Critical Care Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai,China.

Background: Early identification and timely management of septic AKI continue to represent clinical challenges for intensive care. The aim was to evaluate the effect of renal replacement with oXiris filter on clinical outcomes in septic AKI.

Methods: This was a single-center randomized controlled trial that enrolled surgical septic shock with AKI patients admitted in the ICU, Renji Hospital, Shanghai Jiao Tong University, School of Medicine from Jan 1, 2021 to Sep 30, 2021, were screened.

Results: Sixteen subjects that met the inclusion and exclusion criteria were randomized into CRRT with AN69-oXiris group ( = 8) and AN69-ST group ( = 8). The PCT and IL-6 concentration decreased significantly after the first treatment compared to pre-CRRT levels in the oXiris group (PCT: 23.46 [4.18, 84.90] vs 52.79 [9.03, 100.00] µg/L,  = 0.046; IL-6: 3080.15 [527.62, 9806.61] vs 10,457.17 [8150.00, 15,528.87] pg/mL,  = 0.043). The levels of lactate decreased by 1.70 [1.03, 2.83] mmol/L after the first CRRT in the oXiris group ( = 0.028). The norepinephrine infusion rate was decreased by 0.06 [0, 0.09], 0.05 [0, 0.23] and 0.11 [0, 0.23] μg/kg/min at 4, 6, and 8 h in the oXiris group compared to the ST group ( = 0.005, 0.038, and 0.017).

Conclusion: Using the oXiris filter may improve hemodynamic status during initial CRRT in severe surgical septic shock with AKI. Further large multicenter RCTs are needed to determine the effect of the oXiris filter on patient outcomes. (http://www.chictr.org.cn/index.aspx (ChiCTR2200055732)).
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http://dx.doi.org/10.1177/03913988221107947DOI Listing
July 2022

Kukoamine A inhibits C-C motif chemokine receptor 5 to attenuate lipopolysaccharide-induced lung injury.

Drug Dev Res 2022 Jul 21. Epub 2022 Jul 21.

Department of Pediatrics, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The aim of this study was to elucidate the mechanism underlying the effects of Kukoamine A (KuA) treatment on endotoxin-induced lung injury/inflammation. The study was performed in lipopolysaccharide (LPS)-exposed mouse models of lung injury and LPS-induced alveolar epithelial cell model. Relevant kits were used to detect levels of inflammation-related indicators, oxidative stress indicators, and mitochondrial function. Hematoxylin and eosin staining was to detect lung injury. Then, C-C motif chemokine receptor 5 (CCR5) overexpression plasmid was transfected into alveolar epithelial cells to investigate the mechanism of KuA in lung injury. The results showed that LPS induction increased the expression of inflammatory factors, oxidative stress markers, and mitochondrial dysfunction in both animal and cellular models. In the mouse model, KuA treatment improved lung tissue injury, decreased wet-to-dry ratio and MPO levels, reduced the expression of inflammatory factors, and ameliorated oxidative stress and mitochondrial dysfunction. The protective effect of KuA in the cell model remained whereas was markedly reversed after CCR5 overexpression. Taken together, KuA might improve LPS-induced lung injury by inhibiting CCR5. This might also provide a novel theory for KuA in the treatment of lung injury.
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http://dx.doi.org/10.1002/ddr.21975DOI Listing
July 2022

Tailoring the Hydrophilicity for Delayed Condensation Frosting in Antifogging Coatings.

ACS Appl Mater Interfaces 2022 Aug 21;14(30):35064-35073. Epub 2022 Jul 21.

Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Pohang 37673, Republic of Korea.

In the last few decades, numerous studies have focused on designing suitable hydrophilic materials to inhibit surface-induced fog or frost under extreme conditions. As fogging and condensation frosting on a film involves molecular interaction with water prior to forming discrete droplets on the surface, it is essential to control the extent of a film to strongly bind with water molecules for antifogging coatings. While the water contact angle measurement is commonly used to probe the hydrophilicity of a film, it oftentimes fails to predict the antifogging and antifrosting performance as this value only reflects the wettability of a given surface to water droplet. In this work, a polysaccharide-based film composed of chitosan (CHI) and carboxymethyl cellulose (CMC) is used as the model system and oligo(ethylene glycol) (OEG) moieties are additionally introduced to study the effect of OEG moieties on antifogging and condensation frosting. We show that the film containing OEG-grafted CHI exhibits excellent frost-resistant capability due to the OEG moieties in the film that serve as active sites for water molecules to strongly interact in a nonfreezable state.
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http://dx.doi.org/10.1021/acsami.2c07316DOI Listing
August 2022

Cognitive Performance is Associated with Altered Cerebral Hemodynamics Assessed by Transcranial Ultrasound in Parkinson's Disease.

Neuropsychiatr Dis Treat 2022 12;18:1421-1431. Epub 2022 Jul 12.

Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.

Purpose: Cognitive impairment (CI) is a common but debilitating non-motor symptom in Parkinson's disease (PD). Although cerebrovascular functions are related to cognitive performance in healthy individuals, such a relation in PD remains elusive. This study aims to assess the association between cerebrovascular function and cognitive performance in PD individuals.

Patients And Methods: Two-hundred-and-one PD individuals were retrospectively included. They were subsequently divided into two groups: PD with normal cognition (PD-NC) and PD with CI (PD-CI). Cerebral hemodynamic characteristics of the middle cerebral arteries were assessed by transcranial ultrasound. The association between scores in each cognitive domain and cerebral hemodynamic parameters was further analyzed using regression analyses. Additionally, a binary logistic regression model with backward stepwise procedure was applied to build the model for discriminating CI in PD individuals. An independent dataset of additional 46 PD individuals was used further.

Results: The PD-CI group showed a relatively lower end-diastolic blood flow velocity (EDV, < 0.05) and a higher resistive index (RI, < 0.05) compared to the PD-NC group. RI showed significant associations with the memory item score of Montreal Cognitive Assessment ( < 0.05). A model combining clinical and hemodynamic variables was established with optimal efficiency (area under the curve, AUC = 0.651). Further replication of the model in an independent dataset yielded a great consistency (AUC = 0.704).

Conclusion: In our study, cerebrovascular functions were significantly associated with the cognitive performance in PD individuals, especially with the memory task. The established model was effective in identifying CI in PD individuals, which might be a potentially useful tool to screen the cognitive decline in PD individuals at an early stage of the disease. Further studies with larger sample sizes in different populations are warranted.
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http://dx.doi.org/10.2147/NDT.S358150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288215PMC
July 2022
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