Publications by authors named "Wen Hou"

103 Publications

Capecitabine Can Induce T Cell Apoptosis: A Potential Immunosuppressive Agent With Anti-Cancer Effect.

Front Immunol 2021 7;12:737849. Epub 2021 Sep 7.

Organ Transplant Department, Tianjin First Central Hospital, School of Medicine, Nankai University, Tianjin, China.

Capecitabine (CAP) is now widely used in the comprehensive treatment of digestive system tumors. Some clinical observations have shown that CAP may have immunosuppressive effects, but there is still a lack of clear experimental verification. In this study, different doses of CAP were administered to normal mice by gavage. Our results confirmed that CAP did not cause myelosuppression in bone marrow tissue; CAP selectively reduced the proportion of T cells and the concentration of related pro-inflammatory cytokines, while it increased the concentration of anti-inflammatory cytokines. Thymidylate phosphorylase (TP) is the key enzyme for the transformation of CAP ; this study confirmed that T cells express TP, but the bone marrow tissue lacks TP expression, which explains the selectivity in pharmacodynamic effects of CAP. In addition, it was confirmed that CAP can induce T cell apoptosis and experiments showed that CAP-induced T cell apoptosis was related to TP expression, endoplasmic reticulum stress (ERS) induction, reactive oxygen species (ROS) production, and mitochondria-mediated apoptosis activation. Therefore, this study confirmed that the differential expression of TP in cells and tissues explains why CAP avoids the toxic effects of myelosuppression while inducing T cell apoptosis to exert the immunosuppressive effect. Therefore, CAP may become an immunosuppressive agent with a simultaneous anti-cancer effect, which is worthy of further studies.
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http://dx.doi.org/10.3389/fimmu.2021.737849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452994PMC
September 2021

Synthetic cajaninstilbene acid derivatives eradicate methicillin-resistant Staphylococcus aureus persisters and biofilms.

Eur J Med Chem 2021 Jul 10;224:113691. Epub 2021 Jul 10.

College of Pharmacy, Jinan University, Guangzhou, 510632, PR China. Electronic address:

The Staphylococcus aureus can switch to a transient genotype-invariant dormancy, known as a persister, to survive treatment with high doses of antibiotics. This transient persister is an important reason underlying its resistance. There is an urgent need to find new antibacterial agents capable of eradicating methicillin-resistant S. aureus (MRSA) persisters. In this study, 37 new derivatives of cajaninstilbene acid (CSA) were designed and synthesized, and their biological activity against MRSA persisters was evaluated. Most of the newly synthesized derivatives exhibit more potent antimicrobial properties against S. aureus and MRSA than CSA itself, and 23 of the 37 derivatives show a tendency to eradicate MRSA persisters. A representative compound (A6) was demonstrated to target bacterial cell membranes. It eradicated the adherent biofilm of MRSA in a concentration dependent manner, and showed a synergistic antibacterial effect with piperacilin. In a model mouse abscess caused by MRSA persisters, A6 effectively reduced the bacterial load in vivo. These results indicate that A6 is a potential candidate for treatment of MRSA persister infections.
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http://dx.doi.org/10.1016/j.ejmech.2021.113691DOI Listing
July 2021

Hidden Markov Modeling Reveals Prolonged "Baseline" State and Shortened Antagonistic State across the Adult Lifespan.

Cereb Cortex 2021 Jul 13. Epub 2021 Jul 13.

Research Center of Brain and Cognitive Neuroscience, Liaoning Normal University, Dalian 116029, China.

The brain networks undergo functional reorganization across the whole lifespan, but the dynamic patterns behind the reorganization remain largely unclear. This study models the dynamics of spontaneous activity of large-scale networks using hidden Markov model (HMM), and investigates how it changes with age on two adult lifespan datasets of 176/157 subjects (aged 20-80 years). Results for both datasets showed that 1) older adults tended to spend less time on a state where default mode network (DMN) and attentional networks show antagonistic activity, 2) older adults spent more time on a "baseline" state with moderate-level activation of all networks, accompanied with lower transition probabilities from this state to the others and higher transition probabilities from the others to this state, and 3) HMM exhibited higher sensitivity in uncovering the age effects compared with temporal clustering method. Our results suggest that the aging brain is characterized by the shortening of the antagonistic instances between DMN and attention systems, as well as the prolongation of the inactive period of all networks, which might reflect the shift of the dynamical working point near criticality in older adults.
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http://dx.doi.org/10.1093/cercor/bhab220DOI Listing
July 2021

STING1 Promotes Ferroptosis Through MFN1/2-Dependent Mitochondrial Fusion.

Front Cell Dev Biol 2021 14;9:698679. Epub 2021 Jun 14.

The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Ferroptosis is a type of iron-dependent regulated cell death caused by the disruption that occurs when oxidative stress and antioxidant defenses interact, and then driven by lipid peroxidation and subsequent plasma membrane ruptures. The regulation of ferroptosis involves many factors, including the crosstalk between subcellular organelles, such as mitochondria, endoplasmic reticulum (ER), lysosomes, lipid droplets, and peroxisomes. Here, we show that the ER protein STING1 (also known as STING or TMEM173) promotes ferroptosis in human pancreatic cancer cell lines by increasing MFN1/2-dependent mitochondrial fusion, but not mitophagy-mediated mitochondrial removal. The classic ferroptosis inducer erastin, but not sulfasalazine, induces the accumulation of STING1 in the mitochondria, where it binds to MFN1/2 to trigger mitochondrial fusion, leading to subsequent reactive oxygen species production and lipid peroxidation. Consequently, or xenograft mouse models show that the genetic depletion of STING1 or MFN1/2 (but not the mitophagy regulator PINK1 or PRKN) reduces the sensitivity of pancreatic cancer cells to ferroptosis. These findings not only establish a new mitochondrial fusion-dependent cell death mechanism, but also indicate a potential strategy for enhancing ferroptosis-based therapy.
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http://dx.doi.org/10.3389/fcell.2021.698679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236825PMC
June 2021

Radiation-Induced Senescence in p16+/LUC Mouse Lung Compared to Bone Marrow Multilineage Hematopoietic Progenitor Cells.

Radiat Res 2021 Sep;196(3):235-249

Department of Radiation Oncology, UPMC Hillman Cancer Center, Pittsburgh, Pennsylvania 15232.

We defined the time course of ionizing radiation-induced senescence in lung compared to bone marrow of p16+/LUC mice in which the senescence-induced biomarker (p16) is linked to a luciferase reporter gene. Periodic in situ imaging revealed increased luciferase activity in the lungs of 20 Gy thoracic irradiated, but not 8 Gy total-body irradiated (TBI) mice beginning at day 75 and increasing to day 170. In serial sections of explanted lungs, senescent cells appeared in the same areas as did fibrosis in the 20 Gy thoracic irradiated, but not the 8 Gy TBI group. Lungs from 8 Gy TBI mice at one year did show increased RNA levels for p16, p21, p19 and TGF-β. Individual senescent cells in 20 Gy irradiated mouse lung included those with epithelial, endothelial, fibroblast and hematopoietic cell biomarkers. Rare senescent cells in the lungs of 8 Gy TBI mice at one year were of endothelial phenotype. Long-term bone marrow cultures (LTBMCs) were established at either day 60 or one year after 8 Gy TBI. In freshly removed marrow at both times after irradiation, there were increased senescent cells. In LTBMCs, there were increased senescent cells in both weekly harvested single cells and in colonies of multilineage hematopoietic progenitor cells producing CFU-GEMM (colony forming unit-granulocyte, erythrocyte, monocyte/macrophage, mega-karyocyte) that were formed in secondary cultures when these single cells were plated in semisolid media. LTBMCs from TBI mice produced fewer CFU-GEMM; however, the relative percentage of senescent cell-containing colonies was increased as measured by both p16-luciferase and β-galactosidase. Therefore, 20 Gy thoracic radiation, as well as 8 Gy TBI, induces senescent cells in the lungs. With bone marrow, 8 Gy TBI induced senescence in both hematopoietic cells and in colony-forming progenitors. The p16+/LUC mouse strain provides a valuable system in which to compare the kinetics of radiation-induced senescence between organs in vivo, and to evaluate the potential role of senescent cells in irradiation pulmonary fibrosis.
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http://dx.doi.org/10.1667/RADE-20-00286.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456367PMC
September 2021

The type 2 diabetes mellitus susceptibility gene CDKAL1 polymorphism is associated with depressive symptom in first-episode drug-naive schizophrenic patients.

Hum Psychopharmacol 2021 Sep 15;36(5):e2790. Epub 2021 Apr 15.

Research Center of Biological Psychiatry, Suzhou Guangji Hospital, The Affiliated Guangji Hospital of Soochow University, Soochow Unversity, Suzhou, Jiangsu, PR China.

Background: Patients with schizophrenia have an increased prevalence of type 2 diabetes mellitus that has shown a significant association with the rs7754840 polymorphism in the gene encoding the cyclin-dependent kinase 5 (CDK5) regulatory subunit-associated protein 1-like 1 (CDKAL1).

Objective: To examine whether this polymorphism was involved in the susceptibility in first-episode drug-naive schizophrenic patients (FDSP), and further influenced their clinical symptoms.

Methods: This polymorphism was genotyped in 239 FDSP and 368 healthy controls. The clinical symptoms in FDSP were assessed using the Positive and Negative Syndrome Scale (PANSS) five-factor models.

Results: There was no significant difference in the allelic and genotypic frequencies of this polymorphism between two groups (both p > 0.05) after adjusting for covariates. However, the PANSS depressive score significantly differed by genotype in FDSP after adjusting for covariates (F = 5.25, p = 0.006). This significant difference also persisted after Bonferroni correction (p < 0.05). FDSP with C/C genotype had significantly higher PANSS depressive score than those with C/G genotype (p = 0.007) and those with G/G genotype (p = 0.005). Moreover, further stepwise multivariate regression analysis showed the significant association between the rs7754840 polymorphism and PANSS depressive score in FDSP (β = -1.07, t = -2.75, p = 0.007).

Conclusions: Our findings demonstrated that although the CDKAL1 rs7754840 polymorphism did not contribute to the susceptibility to FDSP, it might be implicated in depressive symptoms in this patient group.
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http://dx.doi.org/10.1002/hup.2790DOI Listing
September 2021

Allogeneic adipose-derived stem cells mitigate acute radiation syndrome by the rescue of damaged bone marrow cells from apoptosis.

Stem Cells Transl Med 2021 Jul 16;10(7):1095-1114. Epub 2021 Mar 16.

Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Acute radiation syndrome (ARS) is the radiation toxicity that can affect the hematopoietic, gastrointestinal, and nervous systems upon accidental radiation exposure within a short time. Currently, there are no effective and safe approaches to treat mass population exposure to ARS. Our study aimed to evaluate the therapeutic potential of allogeneic adipose-derived stem cells (ASCs) for total body irradiation (TBI)-induced ARS and understand the underlying mitigation mechanism. We employed 9.25 Gy TBI dose to C57BL/6 mice and studied the effect of allogeneic ASCs on mice survival and regeneration of the hematopoietic system. Our results indicate that intraperitoneal-injected ASCs migrated to the bone marrow, rescued hematopoiesis, and improved the survival of irradiated mice. Our transwell coculture results confirmed the migration of ASCs to irradiated bone marrow and rescue hematopoietic activity. Furthermore, contact coculture of ASCs improved the survival and hematopoiesis of irradiated bone marrow in vitro. Irradiation results in DNA damage, upregulation of inflammatory signals, and apoptosis in bone marrow cells, while coculture with ASCs reduces apoptosis via activation of DNA repair and the antioxidation system. Upon exposure to irradiated bone marrow cells, ASCs secrete prosurvival and hematopoietic factors, such as GM-CSF, MIP1α, MIP1β, LIX, KC, 1P-10, Rantes, IL-17, MCSF, TNFα, Eotaxin, and IP-10, which reduces oxidative stress and rescues damaged bone marrow cells from apoptosis. Our findings suggest that allogeneic ASCs therapy is effective in mitigating TBI-induced ARS in mice and may be beneficial for clinical adaptation to treat TBI-induced toxicities. Further studies will help to advocate the scale-up and adaptation of allogeneic ASCs as the radiation countermeasure.
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http://dx.doi.org/10.1002/sctm.20-0455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235137PMC
July 2021

Diverse Functions of γδ T Cells in the Progression of Hepatitis B Virus and Hepatitis C Virus Infection.

Authors:
Wen Hou Xiaoli Wu

Front Immunol 2020 1;11:619872. Epub 2021 Feb 1.

School of Life Sciences, Tianjin University, Tianjin, China.

Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections are primary risk factors for a wide spectrum of liver diseases that severely affect human health. The liver is an immunological organ that has an abundance of immune cells. Thus, various innate or adaptive immune cells are involved in the progression of HBV or HCV infection. Among those cells, a unique kind of immune cell, the γδ T cell, contributes to promoting or inhibiting the progression of liver diseases. To reveal the diverse roles of γδ T cells in HBV or HCV infection, the properties and functions of these cells in human and mouse models are analyzed. Here, we briefly describe the characteristics and functions of γδ T cells subsets in liver diseases. Then, we fully discuss the diverse roles of γδ T cells in the progression of HBV or HCV infection, including stages of acute infection, chronic infection, liver cirrhosis, and hepatocellular carcinoma. Finally, the functions and existing problems of γδ T cells in HBV or HCV infection are summarized. A better understanding of the function of γδ T cells during the progression of HBV and HCV infection will be helpful for the treatment of virus infection.
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http://dx.doi.org/10.3389/fimmu.2020.619872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882476PMC
June 2021

Mitochondrial DUT-M potentiates RLR-mediated antiviral signaling by enhancing VISA and TRAF2 association.

Mol Immunol 2021 04 11;132:117-125. Epub 2021 Feb 11.

Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, 330022, China. Electronic address:

Upon recognition of intracytoplasmic viral RNA, activated RIG-I is recruited to the mitochondrion-located adaptor protein VISA (also known as MAVS, CARDIF, and IPS-1). VISA then acts as a central signaling platform for linking RIG-I and downstream signaling components, such as TRAF2, 5, and 6, TBK1, and IKK, leading to activation of the kinases TBK1 and IKK. These activated kinases further phosphorylate the transcription factors IRF3/7 and NF-κB, leading to the induction of downstream antiviral genes. Here, we report a mitochondrial isoform, deoxyuridine triphosphate nucleotidohydrolase (dUTPase), DUT-M, as a positive regulator in RLR-VISA-mediated antiviral signaling. DUT-M interacts with VISA and RIG-I to facilitate the assembly of the VISA-TRAF2 complex and to augment the polyubiquitination of TRAF2, leading to potentiated activation of IRF3 dimerization and phosphorylation of P65, and enhanced VISA-mediated innate immune response. RLR-VISA-mediated IRF3 dimerization and P65 phosphorylation, were inhibited in DUT-knockdown and DUT-deficient 293 cells. Thus, DUT-M is a positive regulator of the RIG-I-VISA-mediated innate immune response to RNA viruses.
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http://dx.doi.org/10.1016/j.molimm.2021.01.023DOI Listing
April 2021

Induction of differentiation of the acute myeloid leukemia cell line (HL-60) by a securinine dimer.

Cell Death Discov 2020 Nov 12;6(1):123. Epub 2020 Nov 12.

International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guangzhou, 510632, PR China.

Differentiation therapy has been successfully applied clinically in cases of acute promyelocytic leukemia (APL), but few differentiation-induction agents other than all-trans retinoic acid (ATRA) have been discovered clinically. Based on our previously reported neuritogenic differentiation activity of synthetic dimeric derivatives of securinine, we explored the leukemia differentiation-induction activity of such as compound, SN3-L6. It was found that SN3-L6 induces transdifferentiation of both acute myeloid leukemia (AML) and chronic myelogenous leukemia (CML) cells but unexpectedly, a new transdifferentiation pathway from APL cells to morphologically and immunologically normal megakaryocytes and platelets were discovered. SN3-L6 fails to induce transdifferentiation of ATRA-produced mature granulocytes into megakaryocytes, indicating its selectivity between mature and immature cells. SN3-L6 induces CML K562 cells to transdifferentiate into apoptotic megakaryocytes but without platelet formation, indicating a desirable selectivity between different leukemia cells. Our data illuminate a differentiation gap between AML cells and platelets, and promises applications in leukemia differentiation therapy strategy.
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http://dx.doi.org/10.1038/s41420-020-00354-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665178PMC
November 2020

[Isolation, structural identification of secondary metabolites from Pseudomonas aeruginosa HBD-12 with antibacterial and antitumor activities].

Sheng Wu Gong Cheng Xue Bao 2020 Nov;36(11):2451-2458

College of Biological Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.

To screen strains with antibacterial and antitumor activity, pregnenolone was used as the sole carbon source for screening bacteria from soil. Based on bacteriostatic activity assay, Pseudomonas aeruginosa HBD-12 was found to be effectively inhibiting the growth of Escherichia coli, Bacillus thuringiensis, Penicillium digitatum and Penicillium italicum, and its fermentation broth was separated and purified using column chromatography. Then, structure of the obtained monomeric compounds was analyzed by spectrum analysis, and their antitumor activity was measured using HTRF kinase detection kit. The isolated monomeric compounds 1-hydroxy-9,10-phenanthroline and 3-hydroxy-9,10-dihydrophenanthroline had significant antitumor activity. At 20 μg/mL, 1-hydroxy-9,10-phenanthroline and 3-hydroxy-9,10-dihydrophenanthroline inhibited 78.39±2.29% and 60.34±8.35% Aurora kinase A, respectively. Therefore, the secondary metabolites of Pseudomonas aeruginosa HBD-12 have the potential to develop antibacterial and antitumor drugs.
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http://dx.doi.org/10.13345/j.cjb.200160DOI Listing
November 2020

Associations among gonadal hormone, triglycerides and cognitive decline in female patients with major depressive disorders.

J Psychiatr Res 2020 Nov 9. Epub 2020 Nov 9.

School of Mental Health, First School of Clinical Medicine, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, PR China; Research Center of Biological Psychiatry, The Affiliated Guangji Hospital of Soochow University, Soochow University, Suzhou, 215137, Jiangsu, PR China. Electronic address:

Background: Cognitive impairment has been identified as a core feature of depression. Serum triglycerides (TG), gonadal hormone and sex difference were shown to influence cognitive performance. The purpose of this study was to investigate the associations among serum TG, gonadal hormone, sex difference and cognitive performance in patients with major depressive disorders (MDD).

Methods: The enrolled 183 patients (male/female = 80/103) meeting DSM-IV criteria for MDD were divided into high TG group (patients-HTG) and normal TG group (patients-NTG) according to TG level. Serum TG, estradiol (E2) and testosterone (T) levels were measured by the glycerokinase peroxidase-peroxidase and chemiluminescence methods. Cognition was assessed by the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). The study was conducted between August 2016 and January 2020.

Results: In female, patients-HTG had lower immediate memory, language, attention, delayed memory and RBANS total scores than patients-NTG after adjusting for covariates. There were significant differences in serum E2 and T levels between patients-HTG and patients-NTG in female after controlling for covariates. In female patients-HTG, serum E2 level was positively associated with immediate memory, delayed memory and RBANS total scores, and serum T level was positively related to immediate memory, language and RBANS total scores. These findings were not seen in male patients.

Conclusions: Our data suggested that patients-HTG exhibited poorer cognitive function compared with patients-NTG in female. Moreover, the decline in serum gonadal hormone level might contribute to the high TG development of female MDD, and was further implicated in their cognitive decline.
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http://dx.doi.org/10.1016/j.jpsychires.2020.11.022DOI Listing
November 2020

Fecitrate converted from FeO particles in coal-fired flue gas promoted microalgal biomass and lipid productivities.

Sci Total Environ 2021 Mar 6;760:143405. Epub 2020 Nov 6.

Division of Energy Systems Research, Ajou University, Suwon, Republic of Korea.

In order to reutilize FeO particles in flue gas from coal-fired power plant as a ferrum nutrient for improving microalgae growth, Na-Citrate was proposed to chelate FeCl derived from FeO and HCl reactions to promote biomass and lipid productivities of Chlorella PY-ZU1. Fe-Citrate gave much higher biomass and lipid productivities than FeCl, Fe-EDTA, Fe-DTPA and Fe-HEDTA, because organic chelator prevented Fe from depositing, lower stability constant resulted in easier dissociation of ferric chelate, smaller chelate facilitated Fe (reduced from Fe) transportation through cell membranes. The biomass growth and photosynthetic capacity of Chlorella PY-ZU1 cultivated with Fe-Citrate (converted from FeO particles) medium were similar to those with commercial ferric ammonium citrate medium. The biomass and lipid productivities of Chlorella PY-ZU1 cultivated with 5 mg L Fe-Citrate medium were 1.30 and 1.72 times, respectively, higher than those with FeCl growth medium.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143405DOI Listing
March 2021

A Modified Higher-Order Singular Value Decomposition Framework With Adaptive Multilinear Tensor Rank Approximation for Three-Dimensional Magnetic Resonance Rician Noise Removal.

Front Oncol 2020 11;10:1640. Epub 2020 Sep 11.

Key Laboratory of Biorheological Science and Technology of Ministry of Education, Chongqing University, Chongqing, China.

The magnetic resonance (MR) images are acknowledged to be inevitably corrupted by Rician distributed noise, which adversely affected the image quality for diagnosis purpose. However, the traditional denoising methods may recover the images from corruptions with severe loss of detailed structure and edge information, which would affect the lesion detections and diagnostic decision making. In this study, we challenged improving the Rician noise removal from three-dimensional (3D) MR volumetric data through a () method. The proposed framework of involved a parameterized logarithmic nonconvex penalty function for low-rank tensor approximation (LRTA) algorithm optimization to suppress the image noise in MR dataset. Reference cubes were extracted from the noisy image volume, and block matching was performed according to nonlocal similarity for a fourth-order tensor construction. Then the LRTA problem was implemented by tensor factorization approaches, and the ranks of unfolding matrices along different modes of the tensor were estimated utilizing an adaptive nonconvex low-rank method. The denoised MR images were finally restored through aggregating all recovered cubes. We investigated the proposed algorithm on both the synthetic and real clinic 3D MR images for Rician noise removal, and relative results demonstrated that the can recover images with fine structures and detailed edge preservation with heavy noise even as high as 15% of the maximum intensity. The experimental results were also compared along with several classical denoising methods; the exhibited a sufficient improvement in noise-removal performance at various noise conditions in terms of different measurement indices such as peak signal-to-noise ratio and structural similarity index metrics. Based upon the comparison, the proposed has proved a relative state-of-the-art performance with excellent detailed structure reservation.
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http://dx.doi.org/10.3389/fonc.2020.01640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518100PMC
September 2020

Cerebral blood microcirculation measurement in APP/PS1 double transgenic mice at the preclinical stage of Alzheimer's disease: preliminary data on the early intervention of anodal transcranial direct current stimulation

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:3557-3560

Anodal transcranial direct current stimulation (AtDCS) can improve memory and cognitive dysfunction in patients with Alzheimer's disease (AD), which has been proven in basic and clinical studies. Intervention of AD in preclinical stage is important to prevent progression of AD in the aging society. At the same time, there is increasing evidence that a close link exists between cerebrovascular dysfunction and AD disease. Here we investigated the changes of local cerebral blood microcirculation in preclinical AD mouse model after AtDCS based on the previous studies. Twenty-four 6-month-old male APP/PS1 double transgenic mice were randomly divided into three groups: a model group (AD), a model sham stimulation (ADST) group and a model stimulation group (ATD). Eight 6-month-old male C57 wild-type mice served as a control group (CTL). Mice in the ATD group received 10 AtDCS sessions. Two months after the end of AtDCS in the ATD group, the microcirculation parameters of the frontal cortex of the mice in each group, including cerebral blood flow (CBF), blood flow velocity (Velo), oxygen saturation (SO) and relative hemoglobin content (rHb), were obtained by the non-invasive laser-Doppler spectrophotometry system "Oxygen-to-See (O2C)". The results showed that AtDCS increased CBF, Velo and SO, and reduce rHb in APP/PS1 double transgenic mice at the preclinical stage of AD.Clinical Relevance-This shows the positive effect of AtDCS on preclinical AD in cerebrovascular function, and provides effective basic research facts for AtDCS to intervene and delay the clinical application of AD disease.
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http://dx.doi.org/10.1109/EMBC44109.2020.9175875DOI Listing
July 2020

3D MR image denoising using a modified adaptive high order singular value decomposition method

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:1580-1583

Magnetic resonance (MR) images are generally degraded by random noise governed by Rician distributions. In this study, we developed a modified adaptive high order singular value decomposition (HOSVD) method, taking consideration of the nonlocal self-similarity and weighted Schatten p-norm. We extracted 3D cubes from noise images and classified the similar cubes by the Euclidean distance between cubes to construction a fourth-order tensor. Each rank of unfolding matrices was adaptively determined by weighted Schatten p-norm regularization. The latent noise-free 3D MR images can be obtained by an adaptive HOSVD. Denoising experiments were tested on both synthetic and clinical 3D MR images, and the results showed the proposed method outperformed several existing methods for Rician noise removal in 3D MR images.
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http://dx.doi.org/10.1109/EMBC44109.2020.9175418DOI Listing
July 2020

The Molecular Basis of Host Selection in a Crucifer-Specialized Moth.

Curr Biol 2020 11 10;30(22):4476-4482.e5. Epub 2020 Sep 10.

Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany. Electronic address:

Glucosinolates (GSs) are sulfur-containing secondary metabolites characteristic of cruciferous plants [1, 2]. Their breakdown products, isothiocyanates (ITCs), are released following tissue disruption by insect feeding or other mechanical damages [3, 4]. ITCs repel and are toxic to generalist herbivores, while specialist herbivores utilize the volatile ITCs as key signals for localizing host plants [5, 6]. However, the molecular mechanisms underlying detection of ITCs remain open. Here, we report that in the diamondback moth Plutella xylostella, a crucifer specialist, ITCs indeed drive the host preference for Arabidopsis thaliana, and the two olfactory receptors Or35 and Or49 are essential for this behavior. By performing gene expression analyses, we identified 12 (out of 59 in total) female-biased Ors, suggesting their possible involvement in oviposition choice. By ectopically expressing these Ors in Xenopus oocytes and screening their responses with 49 odors (including 13 ITCs, 25 general plant volatiles, and 11 sex pheromone components), we found that Or35 and Or49 responded specifically to three ITCs (iberverin, 4-pentenyl ITC, and phenylethyl ITC). The same ITCs also exhibited highest activity in electroantennogram recordings with female antennae and were the strongest oviposition stimulants. Knocking out either Or35 or Or49 via CRISPR-Cas9 resulted in a reduced oviposition preference for the ITCs, while double Or knockout females lost their ITC preference completely and were unable to choose between wild-type A. thaliana and a conspecific ITC knockout plant. We hence conclude that the ITC-based oviposition preference of the diamondback moth for its host A. thaliana is governed by the cooperation of two highly specific olfactory receptors.
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http://dx.doi.org/10.1016/j.cub.2020.08.047DOI Listing
November 2020

Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport.

Autophagy 2021 06 25;17(6):1393-1409. Epub 2020 May 25.

Departments of Pathology, University of Pittsburgh School of Medicine and the University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA.

Recently, we reported that increased expression of CASP9 pro-domain, at the endosomal membrane in response to HSP90 inhibition, mediates a cell-protective effect that does not involve CASP9 apoptotic activity. We report here that a non-apoptotic activity of endosomal membrane CASP9 facilitates the retrograde transport of IGF2R/CI-MPR from the endosomes to the trans-Golgi network, indicating the involvement of CASP9 in endosomal sorting and lysosomal biogenesis. CASP9-deficient cells demonstrate the missorting of CTSD (cathepsin D) and other acid hydrolases, accumulation of late endosomes, and reduced degradation of bafilomycin A-sensitive proteins. In the absence of CASP9, IGF2R undergoes significant degradation, and its rescue is achieved by the re-expression of a non-catalytic mutant. This endosomal activity of CASP9 is potentially mediated by herein newly identified interactions of CASP9 with the components of the endosomal membrane transport complexes. These endosomal complexes include the retromer VPS35 and the SNX dimers, SNX1-SNX5 and SNX2-SNX6, which are involved in the IGF2R retrieval mechanism. Additionally, CASP9 interacts with HGS/HRS/ESCRT-0 and the CLTC (clathrin heavy chain) that participate in the initiation of the endosomal ESCRT degradation pathway. We propose that endosomal CASP9 inhibits the endosomal membrane degradative subdomain(s) from initiating the ESCRT-mediated degradation of IGF2R, allowing its retrieval to transport-designated endosomal membrane subdomain(s). These findings are the first to identify a cell survival, non-apoptotic function for CASP9 at the endosomal membrane, a site distinctly removed from the cytoplasmic apoptosome. Via its non-apoptotic endosomal function, CASP9 impacts the retrograde transport of IGF2R and, consequently, lysosomal biogenesis.: ACTB: actin beta; ATG7: autophagy related 7; BafA1: bafilomycin A; CASP: caspase; CLTC/CHC: clathrin, heavy chain; CTSD: cathepsin D; ESCRT: endosomal sorting complexes required for transport; HEXB: hexosaminidase subunit beta; HGS/HRS/ESCRT-0: hepatocyte growth factor-regulated tyrosine kinase substrate; IGF2R/CI-MPR: insulin like growth factor 2 receptor; ILV: intraluminal vesicles; KD: knockdown; KO: knockout; M6PR/CD-MPR: mannose-6-phosphate receptor, cation dependent; MEF: murine embryonic fibroblasts; MWU: Mann-Whitney U test; PepA: pepstatin A; RAB7A: RAB7, member RAS oncogene family; SNX-BAR: sorting nexin dimers with a Bin/Amphiphysin/Rvs (BAR) domain each; TGN: trans-Golgi network; TUBB: tubulin beta; VPS26: VPS26 retromer complex component; VPS29: VPS29 retromer complex component; VPS35: VPS35 retromer complex component.
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http://dx.doi.org/10.1080/15548627.2020.1761742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204962PMC
June 2021

Functional characterization of two spliced variants of fructose gustatory receptor in the diamondback moth, Plutella xylostella.

Pestic Biochem Physiol 2020 Mar 10;164:7-13. Epub 2019 Dec 10.

Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Insect gustatory system plays important roles in multiple behaviors including feeding, mating, and oviposition. Gustatory receptors (GRs), located on the dendritic membrane of gustatory sensory neurons (GSNs), are crucial in peripheral coding of non-volatile compounds. However, GRs and their detailed functions remain poorly understood in lepidopteran pests. In the present work, focusing on GR genes of Plutella xylostella, an important worldwide crop pest, we cloned a candidate fructose GR gene that has two spliced variants (PxylGR43a-1 and PxylGR43a-2), and determined the tissue expression profiles by semi-quantitative reverse transcription PCR (RT-PCR). It revealed that both GR variants were highly expressed in antennae and less highly in heads of adults, while PxylGR43a-2 was also weakly expressed in other tested tissues. Functional analyses were further conducted using the Xenopus oocyte system. PxylGR43a-1 and PxylGR43a-2 both responded specifically to the d-fructose among the 12 tested sugar compounds, but PxylGR43a-2 showed much higher current response than PxylGR43a-1. In addition, the proboscis extension reflex (PER) assay was conducted, demonstrating that female moths could respond to d-fructose following stimulation of antenna. Taken together, our study contributes to elucidation of the molecular mechanisms of fructose reception and provides a potential target for development of GR based pest control techniques.
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http://dx.doi.org/10.1016/j.pestbp.2019.12.002DOI Listing
March 2020

Correction: An NIR-responsive mesoporous silica nanosystem for synergetic photothermal-immunoenhancement therapy of hepatocellular carcinoma.

J Mater Chem B 2020 Apr 26;8(15):3150. Epub 2020 Mar 26.

School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, P. R. China.

Correction for 'An NIR-responsive mesoporous silica nanosystem for synergetic photothermal-immunoenhancement therapy of hepatocellular carcinoma' by Han Yang et al., J. Mater. Chem. B, 2020, 8, 251-259.
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http://dx.doi.org/10.1039/d0tb90040kDOI Listing
April 2020

Plasma BDNF and TrkB mRNA in PBMCs Are Correlated With Anti-depressive Effects of 12-Weeks Supervised Exercise During Protracted Methamphetamine Abstinence.

Front Mol Neurosci 2020 6;13:20. Epub 2020 Mar 6.

Hunan Judicial Police Vocational College, Changsha, China.

: The aim of this study was to evaluate the potential neurotrophic factors and expression of neurotrophin receptors in peripheral blood mononuclear cells linked with the antidepressant action of exercise intervention during protracted methamphetamine (METH) abstinence. : A total of 72 male METH addicts, including 47 individuals with depression and 25 individuals without depression, were recruited in this study. Individuals with depression were divided into the depression control group and the depression exercised group. Consequently, 12 weeks of supervised exercise intervention was applied. Depression and anxiety were analyzed; plasma brain-derived neurotrophic factor (BDNF), neuronal growth factor (NGF), neurotrophin-3 (NT-3), NT-4, and proBDNF levels were tested using enzyme-linked immunosorbent assay; the mRNA expressions of TrkA, TrkB-FL, TrkB-T1, TrkCB, and P75NTR in peripheral blood mononuclear cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). : NT-4 plasma levels were correlated with depression ( = -0.330, = 0.005), which remained significant after Bonferroni correction. In addition, the BDNF and NT-3 levels in the plasma were significantly correlated with depression ( = -0.268, = 0.023; = -0.259, = 0.028), but did not reach significance after Bonferroni correction. The BDNF, NT-3, and NT-4 plasma levels were significantly different between the depressive control group and the depressive exercise group using pre-exercise values as the covariate. The fold changes in TrkB-FL and TrkB-T1 mRNA in peripheral blood mononuclear cells between the post-exercise and pre-exercise demonstrated a remarkable decrease (fold change = -11.056 and -39.055). : Exercise intervention can alleviate depression and anxiety during protracted METH abstinence. Decrease in BDNF and the expression of TrkB in peripheral blood mononuclear cells occur following the exercise intervention.
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http://dx.doi.org/10.3389/fnmol.2020.00020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069447PMC
March 2020

Anti-Ferroptosis Drug Enhances Total-Body Irradiation Mitigation by Drugs that Block Apoptosis and Necroptosis.

Radiat Res 2020 05 5;193(5):435-450. Epub 2020 Mar 5.

Department of Radiation Oncology, UPMC Hillman Cancer Center, Pittsburgh, Pennsylvania 15232.

Mitigation of total-body irradiation (TBI) in C57BL/6 mice by two drugs, which target apoptosis and necroptosis respectively, increases survival compared to one drug alone. Here we investigated whether the biomarker (signature)directed addition of a third anti-ferroptosis drug further mitigated TBI effects. C57BL/6NTac female mice (30-33 g) received 9.25 Gy TBI, and 24 h or later received JP4-039 (20 mg/kg), necrostatin-1 (1.65 mg/kg) and/or lipoxygenase-15 inhibitor (baicalein) (50 mg/kg) in single-, dual- or three-drug regimens. Some animals were sacrificed at days 0, 1, 2, 3, 4 or 7 postirradiation, while the majority in each group were maintained beyond 30 days. For those mice sacrificed at the early time points, femur bone marrow, intestine (ileum), lung and blood plasma were collected and analyzed for radiation-induced and mitigator-modified levels of 33 pro-inflammatory and stress response proteins. Each single mitigator administered [JP4-039 (24 h), necrostatin-1 (48 h) or baicalein (24 h)] improved survival at day 30 after TBI to 25% ( = 0.0432, 0.2816 or 0.1120, respectively) compared to 5% survival of 9.25 Gy TBI controls. Mice were administered the drug individually based on weight (mg/kg). Drug vehicles comprised 30% cyclodextrin for JP4-039 and baicalein, and 10% Cremphor-EL/10% ethanol/80% water for necrostatin-1; thus, dual-vehicle controls were also tested. The dual-drug combinations further enhanced survival: necrostatin-1 (delayed to 72 h) with baicalein 40% ( = 0.0359); JP4-039 with necrostatin-1 50% ( = 0.0062); and JP4-039 with baicalein 60% ( = 0.0064). The three-drug regimen, timed to signature directed evidence of onset after TBI of each death pathway in marrow and intestine, further increased the 30-day survival to 75% ( = 0.0002), and there was optimal normalization to preirradiation levels of inflammatory cytokine and stress response protein levels in plasma, intestine and marrow. In contrast, lung protein levels were minimally altered by 9.25 Gy TBI or mitigators over 7 days. Significantly, elevated intestinal proteins at day 7 after TBI were reduced by necrostatin-1-containing regimens; however, normalization of plasma protein levels at day 7 required the addition of JP4-039 and baicalein. These findings indicate that mitigator targeting to three distinct cell death pathways increases survival after TBI.
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http://dx.doi.org/10.1667/RR15486.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299160PMC
May 2020

Infrared Laser Pulses Excite Action Potentials in Primary Cortex Neurons In Vitro

Annu Int Conf IEEE Eng Med Biol Soc 2019 Jul;2019:5184-5187

Infrared neural modulation (INM) has been well studied in the peripheral nervous system (PNS) for potential clinical applications. However, limited research has been conducted on the central nervous systems (CNS). In this study, we aimed at investigating the feasibility of using pulsed infrared (IR) laser with a wavelength of 1940 nm to excite network activity of cultivated rat cortex neurons.We cultured rat cortex neurons, forming neural networks with spontaneous neural activity, on glass multi-electrode arrays (MEAs). Laser at a power of 600 mW and a pulse rate of 10 Hz were used to stimulate the neural networks using the optics of an inverted microscope. Pulse durations were varied from 200 μs to 1 ms. The spike rate was calculated to evaluate the change of the neural network activity during the IR stimuli and the corresponding frequency components of neural response were calculated to examine whether recorded spikes were evoked by the IR pulse or not. A temperature model was adapted from a previous study to estimate the temperature rise during laser pulsing.We observed that the IR irradiation with a pulse duration of 800 μs and 1 ms could excite neuronal action potentials. The temperature rose 18.5 and 23.9 °C, for pulse durations of 800 μs and 1 ms, respectively. Thus, in addition to previously shown inhibition of IR irradiation with a wavelength of 1550 nm, we demonstrate an optical method that can modulate neural network activity in vitro. The preliminary results from this paper also suggested that MEA recording technology coupled with a laser and microscope systems can be exploited as a new approach for future studies to understand mechanisms and characterize laser parameters of INM for CNS neurons.
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http://dx.doi.org/10.1109/EMBC.2019.8856712DOI Listing
July 2019

Analysis of the Inter-Joints Synergistic Patterns of Limbs in Infant Crawling

Annu Int Conf IEEE Eng Med Biol Soc 2019 Jul;2019:4156-4159

Hands and knees crawling is an important motor developmental milestone, which is characterized by diagonal coordination between upper and lower limbs. However, the features of inter-joint synergy within each limb in infant crawling is still not clear. Therefore, the aim of this study was to extract the inter-joint synergistic patterns during infant crawling and to test the possibilities of using the extracted inter-joint synergy to distinguish developmental delayed (DD) infants from typical developing (TD) infants. In this paper, kinematic data were collected from the shoulder, elbow, wrist, hip, knee, and ankle joints when 9 TD infants and 9 DD infants were crawling on hands and knees at their self-selected velocity. Tangential velocity was firstly calculated from the three-dimensional (3D) trajectory of each joint. Then, the non-negative matrix factorization (NMF) method was used to extract the joints synergistic patterns of each limb from the tangential velocity data. Our preliminary results showed that the crawling movement could be represented by a joint synergistic pattern, which consisted of three joints' data. In addition, we observed that the distal joint had a greater impact than the proximal joints during infant crawling. Moreover, it was found that the DD infants could be preliminarily distinguished from the TD infants by the features of inter-joint synergy during their crawling stage.
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http://dx.doi.org/10.1109/EMBC.2019.8857400DOI Listing
July 2019

Biological evaluation and chemoproteomics reveal potential antibacterial targets of a cajaninstilbene-acid analogue.

Eur J Med Chem 2020 Feb 1;188:112026. Epub 2020 Jan 1.

College of Pharmacy, Jinan University, Guangzhou, 510632, PR China. Electronic address:

Methicillin-resistant Staphylococcus aureus and the formation of persistent nongrowing subpopulations (persisters) is a serious threat to human. Our previous studies have proved that two cajaninstilbene acid (CSA) analogues, compound 5b and 5j display remarkable antibacterial activities, especially overcoming drug resistance of methicillin-resistant Staphylococcus aureus (MRSA). Present study found that 5b and 5j are capable of eradicating MRSA persisters. However, their underlying antibacterial mechanism is still obscure. In this study, biological evaluation was performed by transmission electron micrograph, membrane permeability and membrane depolarization experiment to reveal the effects of drugs on bacteria. Further, affinity-based protein profiling and transcriptional profiling were performed to characterise the protein targets in bacterial. Biological evaluation suggested that 5b has an effect on bacterial membrane, affinity-based protein profiling identified that 5b targets membrane associated protein PgsA and verified by in vitro labelling profile. Transcriptional profiling indicated that 5b interferes in phosphatidylglycerol (PG) synthesis pathway. This study identified a novel antibacterial target PgsA and it might be a potential target to combat the resistant bacteria.
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http://dx.doi.org/10.1016/j.ejmech.2019.112026DOI Listing
February 2020

Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation.

In Vivo 2020 Jan-Feb;34(1):39-50

Department of Radiation Oncology, UPMC Hillman Cancer Center, Pittsburgh, PA, U.S.A.

Background/aim: Intestinal damage induced by total body irradiation (TBI) reduces leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells, goblet, and Paneth cells, breaching the epithelial lining, and facilitating bacterial translocation, sepsis, and death.

Materials And Methods: Survival was measured after TBI in animals that received wild-type or recombinant bacteria producing interleukin-22 (IL-22). Changes in survival due to microbially delivered IL-22 were measured. Lactobacillus reuteri producing IL-22, or Escherichia coli-IL-22 were compared to determine which delivery system is better.

Results: C57BL/6 mice receiving IL-22 probiotics at 24 h after 9.25 Gy TBI, demonstrated green fluorescent protein-positive bacteria in the intestine, doubled the number of Lgr5+ intestinal stem cells, and increased 30-day survival. Bacteria were localized to the jejunum, ileum, and colon.

Conclusion: Second-generation probiotics appear to be valuable for mitigation of TBI, and radiation protection during therapeutic total abdominal irradiation.
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http://dx.doi.org/10.21873/invivo.11743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984118PMC
June 2020

Fanconi Anemia Mouse Genotype-specific Mitigation of Total Body Irradiation by GS-Nitroxide JP4-039.

In Vivo 2020 Jan-Feb;34(1):33-38

Department of Radiation Oncology, UPMC Hillman Cancer Center, Pittsburgh, PA, U.S.A.

Background/aim: Radiation mitigator, GS-nitroxide, JP4-039, was evaluated for mitigation of total body irradiation (TBI) in Fanconi anemia (FA) Fancd2 (129/Sv), Fancg (B6), and Fanca (129/Sv) mice.

Materials And Methods: JP4-039 dissolved in 30% 2-hydroxypropyl-β-cyclodextrin was injected intramuscularly 24 h after total body irradiation (9.25 Gy) into Fanca, Fancd2 and Fancg mice. Irradiation survival curves were performed in vitro using bone marrow stromal cell lines derived from Fanca, Fancd2 and Fancg mice.

Results: FA mice demonstrate genotype specific differences in TBI mitigation by JP4-039. Radiation effects in derived bone marrow stromal cell lines in vitro were mitigated by drugs that block apoptosis, but not necroptosis or ferroptosis.

Conclusion: FA mouse models are valuable for elucidating DNA repair pathways in cell and tissue responses to TBI, and the role of drugs that target distinct cell death pathways.
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http://dx.doi.org/10.21873/invivo.11742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984088PMC
June 2020

The Severe Fever with Thrombocytopenia Syndrome Virus NSs Protein Interacts with CDK1 To Induce G Cell Cycle Arrest and Positively Regulate Viral Replication.

J Virol 2020 02 28;94(6). Epub 2020 Feb 28.

School of Life Sciences, Tianjin University, Tianjin, China

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly identified phlebovirus associated with severe hemorrhagic fever in humans. While many viruses subvert the host cell cycle to promote viral growth, it is unknown whether this is a strategy employed by SFTSV. In this study, we investigated how SFTSV manipulates the cell cycle and the effect of the host cell cycle on SFTSV replication. Our results suggest that cells arrest at the G/M transition following infection with SFTSV. The accumulation of cells at the G/M transition did not affect virus adsorption and entry but did facilitate viral replication. In addition, we found that SFTSV NSs, a nonstructural protein that forms viroplasm-like structures in the cytoplasm of infected cells and promotes virulence by modulating the interferon response, induces a large number of cells to arrest at the G/M transition by interacting with CDK1. The interaction between NSs and CDK1, which is inclusion body dependent, inhibits formation and nuclear import of the cyclin B1-CDK1 complex, thereby leading to cell cycle arrest. Expression of a CDK1 loss-of-function mutant reversed the inhibitive effect of NSs on the cell cycle, suggesting that this protein is a potential antiviral target. Our study provides new insight into the role of a specific viral protein in SFTSV replication, indicating that NSs induces G/M arrest of SFTSV-infected cells, which promotes viral replication. Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne pathogen that causes severe hemorrhagic fever. Although SFTSV poses a serious threat to public health and was recently isolated, its pathogenesis remains unclear. In particular, the relationship between SFTSV infection and the host cell cycle has not been described. Here, we show for the first time that both asynchronized and synchronized SFTSV-susceptible cells arrest at the G/M checkpoint following SFTSV infection and that the accumulation of cells at this checkpoint facilitates viral replication. We also identify a key mechanism underlying SFTSV-induced G/M arrest, in which SFTSV NSs interacts with CDK1 to inhibit formation and nuclear import of the cyclin B1-CDK1 complex, thus preventing it from regulating cell cycle progression. Our study highlights the key role that NSs plays in SFTSV-induced G/M arrest.
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http://dx.doi.org/10.1128/JVI.01575-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158732PMC
February 2020

[Screening, identification and characterization of a broadspectrum antagonistic strain in banyan rhizosphere soil].

Ying Yong Sheng Tai Xue Bao 2019 Nov;30(11):3894-3902

College of Biological Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.

Rhizosphere soil samples were collected from an ancient banyan tree grown in the Wanli period of Ming Dynasty in Ji'an City, Jiangxi Province. Twenty-three kinds of indicator bacteria were used to screen soil actinomycetes by cylinder-plate method and mycelium growth rate method. A broad-spectrum antagonistic strain AHF-20 with stable passage was obtained. According to the morphological observation, physiological and biochemical tests, and molecular biological identification, the antagonistic strain was identified as Streptomyces. We examined the antibacterial active substance of the strain. The results showed that the fermentation products of Streptomyces AHF-20 had antagonistic effects on all the 23 test indicator bacteria. The antibacterial ability was stable, tolerant to temperature, light, ultraviolet, acid and alkali. Antibacterial activity still existed after heating at 121 ℃ for 20 min. The fermentation product was extracted with n-butanol according to the polarity of the active substance. The obtained crude n-butanol extract was diluted to 1 μg·mL, which still had inhibitory effect for Escherichia coli. The results indicated that it has well utilization potential for biocontrol and developing new microbial drug.
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http://dx.doi.org/10.13287/j.1001-9332.201911.037DOI Listing
November 2019

Prevalence, social-demographic and cognitive correlates of depression in Chinese psychiatric medical staff.

J Affect Disord 2020 02 30;263:60-63. Epub 2019 Nov 30.

Research Center of Biological Psychiatry, Suzhou Guangji Hospital, Suzhou 215137, Jiangsu, China; Kangning Hospital attached to Wenzhou Medical University, Wenzhou 325007, Zhejiang, China; Neuropsychology and Applied Cognitive Neuroscience Laboratory, CAS Key Laboratory of Mental Health Institute of Psychiatry, Beijing 100101, China; The Affiliated Guangji Hospital of Soochow University, Suzhou 215137, Jiangsu, China. Electronic address:

Background: The high prevalence of depression in general population was related to its social-demographics and cognitive performance. However, no studies investigated the prevalence of depression, its social-demographic and cognitive correlates in psychiatric medical staff. Thus, the aims of this study investigated the prevalence, social-demographic and cognitive correlates of depression in Chinese psychiatric medical staff.

Methods: 186 Chinese psychiatric medical staff were enrolled in Wenzhou Kangning Hospital. Depressive symptom score was assessed by the Self-rating Depression Scale (SDS). Cognition was assessed by the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS).

Results: The prevalence of depression was 17.74% in these medical staff. The RBANS total score in participants with depressive symptom was significantly lower than that in participants with not depressive symptom after controlling for the confounding variables. The Person correlation analysis found that the normal SDS score in these medical staff was significantly related to age, education, occupations, RBANS total score and subscale scores. Stepwise multivariate regression analysis further identified that age and RBANS total score were significantly associated with the normal SDS score in these medical staff.

Limitations: The limitations included cross-sectional study design, the small sample size, and the self-rating scale of depression.

Conclusions: The prevalence of depression in Chinese psychiatric medical staff was higher in comparison with Chinese general population, but lower in comparison with Chinese medical staff. Cognitive deficits might be considered a core feather of depression that should be a valuable target for future interventions. Age influenced depressive symptom in these medical staff .
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http://dx.doi.org/10.1016/j.jad.2019.11.133DOI Listing
February 2020
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