Publications by authors named "Wen Hong"

160 Publications

Self-adaptive cardiac optogenetics device based on negative stretching-resistive strain sensor.

Sci Adv 2021 Nov 24;7(48):eabj4273. Epub 2021 Nov 24.

National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Shanghai Jiao Tong University, Shanghai 200240, China.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abj4273DOI Listing
November 2021

Dense Packed Drivable Optrode Array for Precise Optical Stimulation and Neural Recording in Multiple-Brain Regions.

ACS Sens 2021 Nov 15;6(11):4126-4135. Epub 2021 Nov 15.

National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Shanghai Jiao Tong University, Shanghai 200240, China.

The input-output function of neural networks is complicated due to the huge number of neurons and synapses, and some high-density implantable electrophysiology recording tools with a plane structure have been developed for neural circuit studies in recent years. However, traditional plane probes are limited by the record-only function and inability to monitor multiple-brain regions simultaneously, and the complete cognition of neural networks still has a long way away. Herein, we develop a three-dimensional (3D) high-density drivable optrode array for multiple-brain recording and precise optical stimulation simultaneously. The optrode array contains four-layer probes with 1024 microelectrodes and two thinned optical fibers assembled into a 3D-printed drivable module. The recording performance of microelectrodes is optimized by electrochemical modification, and precise implantation depth control of drivable optrodes is verified in agar. Moreover, in vivo experiments indicate neural activities from CA1 and dentate gyrus regions are monitored, and a tracking of the neuron firing for 2 weeks is achieved. The suppression of neuron firing by blue light has been realized through high-density optrodes during optogenetics experiments. With the feature of large-scale recording, optoelectronic integration, and 3D assembly, the high-density drivable optrode array possesses an important value in the research of brain diseases and neural networks.
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http://dx.doi.org/10.1021/acssensors.1c01650DOI Listing
November 2021

New targets of morphine postconditioning protection of the myocardium in ischemia/reperfusion injury: Involvement of HSP90/Akt and C5a/NF-κB.

Open Med (Wars) 2021 18;16(1):1552-1563. Epub 2021 Oct 18.

Department of Geriatric Cardiology, First Affiliated Hospital, Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, Guangxi, China.

Background: Activation of the complement component 5a (C5a) and nuclear factor κB (NF-κB) signaling is an important feature of myocardial ischemia/reperfusion (I/R) injury and recent studies show that morphine postconditioning (MP) attenuates the myocardial injury. However, the mediating cardioprotective mechanisms remain unclear. The present study explores the role and interaction of heat shock protein 90 (HSP90), Akt, C5a, and NF-κB in MP-induced cardioprotection.

Methods: Male Sprague Dawley rats ( = 160) were randomized into eight groups ( = 20 per group). Rats in the sham group underwent thoracotomy, passing the ligature through the heart but without tying it (150 min), and the other seven groups were subjected to 30 min of anterior descending coronary artery occlusion followed by 2 h of reperfusion and the following treatments: I/R (30 min of ischemia and followed by 2 h of reperfusion); ischemic postconditioning (IPostC, 30 s of ischemia altered with 30 s of reperfusion, repeated for three cycles, and followed by reperfusion for 2 h); MP (0.3 mg/kg morphine administration 10 min before reperfusion); MP combined with the HSP90 inhibitor geldanamycin (GA, 1 mg/kg); MP combined with the Akt inhibitor GSK-690693 (GSK, 20 mg/kg); and MP combined with the C5a inhibitor PMX205 (PMX, 1 mg/kg/day, administration via drinking water for 28 days) and MP combined with the NF-κB inhibitor EVP4593 (QNZ, 1 mg/kg). All inhibitors were administered 10 min before morphine and followed by 2 h reperfusion.

Results: MP significantly reduced the I/R-induced infarct size, the apoptosis, and the release of cardiac troponin I, lactate dehydrogenase (LDH), and creatine kinase-MB. These beneficial effects were accompanied by increased expression of HSP90 and p-Akt, and decreased expression of C5a, NF-κB, tumor necrosis factor α, interleukin-1β, and intercellular cell adhesion molecule 1. However, HSP90 inhibitor GA or Akt inhibitor GSK increased the expression of C5a and NF-κB and prevented MP-induced cardioprotection. Furthermore, GA inhibited the MP-induced upregulation of p-Akt, while GSK did not affect HSP90, indicating that p-Akt acts downstream of HSP90 in MP-induced cardioprotection. In addition, C5a inhibitor PMX enhanced the MP-induced downregulation of NF-κB, while NF-κB inhibitor QNZ had no effect on C5a, indicating that the C5a/NF-κB signaling pathway is involved in MP-induced cardioprotection.

Conclusion: HSP90 is critical for MP-mediated cardioprotection possibly by promoting the phosphorylation of Akt and inhibiting the activation of C5a and NF-κB signaling and the subsequent myocardial inflammation, ultimately attenuating the infarct size and cardiomyocyte apoptosis.
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http://dx.doi.org/10.1515/med-2021-0340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525660PMC
October 2021

In Silico Analysis of the Gene Expression Patterns between Aldosterone-Producing Adenoma and Nonfunctional Adrenocortical Adenoma.

Genet Res (Camb) 2021 4;2021:9553637. Epub 2021 Oct 4.

Department of Cardiovascular Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.

Primary aldosteronism is the most common form of secondary hypertension, and aldosteronoma makes up a significant proportion of primary aldosteronism cases. Aldosteronoma is also called aldosterone-producing adenoma (APA). Although there have been many studies about APA, the pathogenesis of this disease is not yet fully understood. In this study, we aimed to find out the difference of gene expression patterns between APA and nonfunctional adrenocortical adenoma (NFAA) using a weighted gene coexpression network (WGCNA) and differentially expressed gene (DEG) analysis; only the genes that meet the corresponding standards of both methods were defined as real hub genes and then used for further analysis. Twenty-nine real hub genes were found out, most of which were enriched in the phospholipid metabolic process. , , , , , and are six real hub genes with the same gene expression pattern between the combined and validation datasets, three of which indirectly or directly participate in lipid metabolism including , , and . According to the gene expression pattern of DEGs, we speculated five candidate drugs with potential therapeutic value for APA, one of which is cycloheximide, an inhibitor for phospholipid biosynthesis. All the evidence suggests that phospholipid metabolism may be an important pathophysiological mechanism for APA. Our study provides a new perspective regarding the pathophysiological mechanism of APA and offers some small molecules that may possibly be effective drugs against APA.
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http://dx.doi.org/10.1155/2021/9553637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505127PMC
October 2021

Tumor-activatable biomineralized nanotherapeutics for integrative glucose starvation and sensitized metformin therapy.

Biomaterials 2021 11 9;278:121165. Epub 2021 Oct 9.

School of Life Science, Chongqing University, Chongqing, 400044, PR China. Electronic address:

Metformin is a clinically-approved anti-diabetic drug with emerging antitumor potential, but its antitumor activity is highly susceptible to local glucose abundance. Herein, we construct a nanotherapeutic platform based on biocompatible constituents to sensitize tumor cells for metformin therapy via cooperative glucose starvation. The nanoplatform was synthesized through the spontaneous biomineralization of glucose oxidase (GOx) and metformin in amorphous calcium phosphate nanosubstrate, which was further modified with polyethylene glycol and cRGD ligands. This biomineralized nanosystem could efficiently deliver the therapeutic payloads to tumor cells in a targeted and bioresponsive manner. Here GOx could catalyze the oxidation of glucose into gluconic acid and HO, thus depleting the glucose in tumor intracellular compartment while accelerating the release of the entrapped therapeutic payloads. The selective glucose deprivation would not only disrupt tumor energy metabolism, but also upregulate the PP2A regulatory subunit B56δ and sensitize tumor cells to the metformin-induced CIP2A inhibition, leading to efficient apoptosis induction via PP2A-GSK3β-MCL-1 axis with negligible side effects. This study may offer new avenues for targeted tumor therapy in the clinical context.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121165DOI Listing
November 2021

Histone mimics: more tales to read.

Biochem J 2021 07;478(14):2789-2791

Department of Epigenetics, Van Andel Institute, 333 Bostwick Ave. NE, Grand Rapids, Michigan 49503, U.S.A.

Post-translational modifications (PTMs) on histone proteins are known as epigenetic marks that demarcate the status of chromatin. These modifications are 'read' by specific reader proteins, which in turn recruit additional factors to modulate chromatin accessibility and the activity of the underlying DNA. Accumulating evidence suggests that these modifications are not restricted solely to histones, many non-histone proteins may function in a similar way through mimicking the histones. In this commentary, we briefly discuss a systematic study of the discovery of histone H3 N-terminal mimicry proteins (H3TMs), and their implications in chromatin regulation and drug discoveries.
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http://dx.doi.org/10.1042/BCJ20210357DOI Listing
July 2021

Interference with the expression of S1PR1 or STAT3 attenuates valvular damage due to rheumatic heart disease.

Int J Mol Med 2021 Sep 23;48(3). Epub 2021 Jul 23.

Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

Rheumatic heart disease (RHD) affects numerous individuals annually; however, its pathogenesis remains unclear. The sphingosine 1‑phosphate receptor 1 (S1PR1) and signal transducer and activator of transcription 3 (STAT3) have recently been shown to be involved in valvular damage via the promotion of the differentiation of T helper 17 (Th17) cells during the development of RHD‑induced valvular damage. The present study investigated whether altering the expression of S1PR1 or STAT3 attenuates valvular damage due to RHD. Inactivated group A streptococcus (GAS) was used to establish a rat model of RHD. Recombinant adeno‑associated viral vectors carrying an S1PR1 overexpression sequence were used to overexpress S1PR1. STAT3 small interfering RNA (STAT3‑siRNA) was used to inhibit STAT3 expression. Reverse transcription‑quantitative PCR (RT‑qPCR) was performed to detect the mRNA expression of S1PR1, STAT3, collagen type III α1 chain (Col3a1) and fibroblast‑specific protein 1. Western blotting (WB) and immunohistochemistry were used to detect the levels of S1PR1, STAT3, phosphorylated (p‑) STAT3, and retinoic acid‑related orphan receptor γT (RORγt) proteins. Enzyme‑linked immunosorbent assays (ELISAs) and immunohistochemistry were used to detect the levels of interleukin (IL)‑6 and IL‑17. Hematoxylin and eosin (H&E) staining and Sirius Red staining were performed to evaluate the degree of inflammation and fibrosis in the valvular tissues. S1PR1 expression was decreased in the valvular tissues of the rats with RHD. The levels of IL‑6, IL‑17 and p‑STAT3 in the rats with RHD were increased. The degree of valvular inflammation and fibrosis in the rats with RHD was also increased. The overexpression of S1PR1 and the inhibition of STAT3 reduced the total p‑STAT3 level, resulting in decreased levels of IL‑6, IL‑17 and RORγt, and a reduced degree of valvular inflammation and fibrosis. These results suggest that the expression of S1PR1 and STAT3 may be involved in valvular tissue damage due to RHD. Thus, strategies designed to interfere with the expression of S1PR1 or STAT3 may affect the expression of Th17 cell‑related cytokines and may thus attenuate valvular damage due to RHD.
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http://dx.doi.org/10.3892/ijmm.2021.5012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354313PMC
September 2021

Discovery of Selective Small-Molecule Inhibitors for the ENL YEATS Domain.

J Med Chem 2021 08 19;64(15):10997-11013. Epub 2021 Jul 19.

Department of Epigenetics, Van Andel Institute, Grand Rapids, Michigan 49503, United States.

Eleven-nineteen leukemia (ENL) protein is a histone acetylation reader essential for disease maintenance in acute leukemias, in particular, the ()-rearranged leukemia. In this study, we carried out high-throughput screening of a small-molecule library to identify inhibitors for the ENL YEATS domain. Structure-activity relationship studies of the hits and structure-based inhibitor design led to two compounds, and , with IC values below 100 nM in inhibiting the ENL-acetyl-H3 interaction. Both compounds, and their precursor compound , displayed strong selectivity toward the ENL YEATS domain over all other human YEATS domains. Moreover, exhibited on-target inhibition of ENL in cultured cells and a synergistic effect with the bromodomain and extraterminal domain inhibitor JQ1 in killing leukemia cells. Together, we have developed selective chemical probes for the ENL YEATS domain, providing the basis for further medicinal chemistry-based optimization to advance both basic and translational research of ENL.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486320PMC
August 2021

The role of the PZP domain of AF10 in acute leukemia driven by AF10 translocations.

Nat Commun 2021 07 5;12(1):4130. Epub 2021 Jul 5.

Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, USA.

Chromosomal translocations of the AF10 (or MLLT10) gene are frequently found in acute leukemias. Here, we show that the PZP domain of AF10 (AF10), which is consistently impaired or deleted in leukemogenic AF10 translocations, plays a critical role in blocking malignant transformation. Incorporation of functional AF10 into the leukemogenic CALM-AF10 fusion prevents the transforming activity of the fusion in bone marrow-derived hematopoietic stem and progenitor cells in vitro and in vivo and abrogates CALM-AF10-mediated leukemogenesis in vivo. Crystallographic, biochemical and mutagenesis studies reveal that AF10 binds to the nucleosome core particle through multivalent contacts with the histone H3 tail and DNA and associates with chromatin in cells, colocalizing with active methylation marks and discriminating against the repressive H3K27me3 mark. AF10 promotes nuclear localization of CALM-AF10 and is required for association with chromatin. Our data indicate that the disruption of AF10 function in the CALM-AF10 fusion directly leads to transformation, whereas the inclusion of AF10 downregulates Hoxa genes and reverses cellular transformation. Our findings highlight the molecular mechanism by which AF10 targets chromatin and suggest a model for the AF10-dependent CALM-AF10-mediated leukemogenesis.
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http://dx.doi.org/10.1038/s41467-021-24418-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257627PMC
July 2021

Postconditioning attenuates myocardial ischemia-reperfusion injury by inhibiting complement activation and upregulation of miR-499.

Exp Ther Med 2021 Jul 26;22(1):684. Epub 2021 Apr 26.

Department of Cardiology, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

The complement system plays a vital role in myocardial ischemia/reperfusion (I/R) injury. microRNA (miR)-499 is involved in the cardioprotection of ischemic postconditioning (IPostC). The present study aimed to study the role of the complement system and miR-499 in IPostC. Rat hearts were subjected to coronary ligation for 30 min, followed by reperfusion for 2 h. IPostC was introduced at the onset of reperfusion with three cycles of reperfusion for 30 sec and coronary artery occlusion for 30 sec. To study the role of miR-499 in IPostC, adeno-associated virus (AAV) vectors of miR-499-5p (AAV-miR-499-5p) and miR-499-5p-sponge (AAV-miR-499-5p-sponge) were transfected via tail vein injection, followed by IPostC protocols. Cardiac injury as well as the status of local and systemic complement activation and inflammation were assessed. IPostC significantly attenuated I/R-induced rat cardiomyocyte apoptosis and the myocardial infarct size. These beneficial effects were accompanied by decreased local and circulating complement component (C)3a and C5a levels, decreased inflammatory marker expression, decreased NF-κB signaling and increased cardiac miR-499 expression. AAV-miR-499-5p prevented local and systemic complement activation and inflammation as well as enhanced the cardioprotection of IPostC, whereas AAV-miR-499-5p-sponge produced the opposite effects. In summary, IPostC protected the rat myocardium against I/R injury, by inhibiting local and systemic complement activation; inflammation; NF-κB signaling; and upregulation of miR-499. As such, miR-499 may have a critical role in IPostC-mediated cardioprotection against I/R injury.
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http://dx.doi.org/10.3892/etm.2021.10116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111864PMC
July 2021

Excess mortality among patients with severe mental disorders and effects of community-based mental healthcare: a community-based prospective study in Sichuan, China.

BJPsych Open 2021 Apr 22;7(3):e84. Epub 2021 Apr 22.

West China School of Public Health, Sichuan University, China; and Faculty of Health, Art and Design, Swinburne University of Technology, Australia.

Background: High-quality primary care reduces premature mortality in the general population, but evidence for psychiatric patients in China is scarce.

Aims: To confirm excess mortality in patients with severe mental illness (SMI), and to examine the impact of community-based mental healthcare and other risk factors on their mortality.

Method: We included 93 655 patients in 2012 and 100 706 in 2013 from the national mental health surveillance system in Sichuan, China to calculate the standardised mortality ratio (SMR). A total of 112 576 patients were followed up from 2009 to 2014 for model analyses. We used growth models to quantify the patterns of change for community management measures, high-risk behaviour, disease stability and medication adherence of patients over time, and then used multilevel proportional hazard models to examine the association between change patterns of management measures and mortality.

Results: The SMR was 6.44 (95% CI 4.94-8.26) in 2012 and 7.57 (95% CI 5.98-9.44) in 2013 among patients with SMI aged 15-34 years, and diminished with age. Unfavourable baseline socioeconomic status increased the hazard of death by 38-50%. Positive changes in high-risk behaviour, disease stability and medication adherence had a 54% (95% CI 47-60%), 69% (95% CI 63-73%) and 20% (4-33%) reduction in hazard of death, respectively, versus in those where these were unchanged.

Conclusions: High excess mortality was confirmed among younger patients with SMI in Sichuan, China. Our findings on the relationships between community management and socioeconomic factors and mortality can inform community-based mental healthcare policies to reduce excess mortality among patients with SMI.
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http://dx.doi.org/10.1192/bjo.2021.46DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086393PMC
April 2021

Source apportionment and health risk assessment of potentially toxic elements in soil from mining areas in northwestern China.

Environ Geochem Health 2021 Mar 31. Epub 2021 Mar 31.

School of Environmental & Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China.

Soil contaminated with toxic elements from mining activities is a public health concern. In order to obtain a comprehensive understanding of the status and potential risks of inorganic toxic elements in soil resulting from mining activities, Cu, Pb, Cr, Zn, Ni, As, and Cd were selected to evaluate a total of 42 soil samples collected from Gannan mining areas in northwestern China. The concentrations of As and Cd were much higher than their respective background values, while the concentrations of the other elements fluctuated around their background values. Results of combined multivariate statistical analyses and the distribution patterns of the individual pollutants imply that the toxic elements were originated from different sources even for one element in different sampling locations. The pollution index values indicated that As and Cd have a moderate to high pollution levels. The geo-accumulation indexes (I) indicated that Cu, Pb, Cr, Zn, and Ni are likely of geologic origin, while As and Cd have been significantly affected by anthropogenic activities. Potential ecological risk indexes further showed that soils from mining areas within the study area pose a high potential ecological risk, and As and Cd were major risk contributors. Based on the calculated Hazard Index, the ingestion of soil particles appeared to be the main exposure route resulting in a higher risk, followed by dermal contact. The potential health risks of children and adults for As were greater than the safe level. The carcinogenic risk associated with As for local residents was also higher than the accepted levels, indicating a serious health risk to local residents. These results suggest that proper management strategies and various remediation practices should be implemented in the Gannan mining area in northwestern China.
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http://dx.doi.org/10.1007/s10653-021-00907-0DOI Listing
March 2021

Cervical erector spinae plane block as a perioperative analgesia method for shoulder arthroscopy: a case series.

J Anesth 2021 06 8;35(3):446-450. Epub 2021 Mar 8.

Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.

Shoulder arthroscopy, a common intervention for severe rotator cuff injuries, is associated with severe postoperative pain. Upon performing cervical erector spinae plane (ESP) blocks at the C7 TP (tip or posterior tip) or the posterior tip of the C6 TP posterior tubercle in six patients undergoing shoulder arthroscopy, sensory block was detectable in congruent cervico-thoracic dermatomes. Effective intraoperative and postoperative analgesia were consistently obtained for all six patients. This preliminary study illustrated that the cervical ESP block can be considered a potential simple regional anesthesia method for providing analgesia during shoulder arthroscopy with low risks of diaphragmatic paresis, upper extremity motor paresis, nerve injury and persistent hypotension.
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http://dx.doi.org/10.1007/s00540-021-02907-xDOI Listing
June 2021

Single and competitive sorption of sulfadiazine and chlortetracycline on loess soil from Northwest China.

Environ Pollut 2020 Aug 27;263(Pt A):114650. Epub 2020 Apr 27.

Key Laboratory of Petroleum Resources Research, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.

The fate of veterinary antibiotics (VAs) in soil environment is determined by the hydrophilic performance and solubility of VAs and the type of soil. In this study, sulfadiazine (SDZ) and chlortetracycline (CTC) were selected as target pollutants, and a batch sorption method was used to find out the single and sorption competitive behavior and mechanism of the target pollutants on loess soil. Kinetic studies showed the apparent sorption equilibrium was reached 0-6 h for CTC and 0-12 h for SDZ. The sorption kinetics of VAs on loess soil were fitted well with a pseudo-second order kinetic model. Sorption thermodynamic data indicated the isotherm sorption of both SDZ and CTC on loess soil was fitted well with Freundlich isothermal (R, 0.960-0.975) and linear models (R, 0.908-0.976). The sorption affinity of CTC (K, 290-1620 L/kg for CTC) was much greater than that of SDZ (K, 0.6-4.9 L/kg for SDZ). The results also suggest that SDZ may be easily mobilized or leached from loess soil at neutral and alkaline pH, while CTC may be easily mobilized or leached at neutral pH. The sorption of each single target pollutant on the outer layer complex decreased with increasing ionic strength. Higher initial concentrations resulted in greater sorption capacity of target pollutants on loess soil increased. The sorption capacities of CTC and SDZ in the mixed system were lower than the sorption capacity of each single system, showing a competitive sorption behavior of CTC and SDZ during the sorption process. Overall, CTC showed the highest sorption potential in loess soil, whereas SDZ showed a high leaching risk in loess soil. These findings contribute to understanding the fate of different VAs in loess in the natural environment.
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http://dx.doi.org/10.1016/j.envpol.2020.114650DOI Listing
August 2020

Frequency Domain Panoramic Imaging Algorithm for Ground-Based ArcSAR.

Sensors (Basel) 2020 Dec 8;20(24). Epub 2020 Dec 8.

Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100049, China.

The ground-based arc-scanning synthetic aperture radar (ArcSAR) is capable of 360° scanning of the surroundings with the antenna fixed on a rotating arm. ArcSAR has much wider field of view when compared with conventional ground-based synthetic aperture radar (GBSAR) scanning on a linear rail. It has already been used in deformation monitoring applications. This paper mainly focuses on the accurate and fast imaging algorithms for ArcSAR. The curvature track makes the image focusing challenging and, in the classical frequency domain, fast imaging algorithms that are designed for linear rail SAR cannot be readily applied. This paper proposed an efficient frequency domain imaging algorithm for ArcSAR. The proposed algorithm takes advantage of the angular shift-invariant property of the ArcSAR signal, and it deduces the accurate matched filter in the angular-frequency domain, so panoramic images in polar coordinates with wide swath can be obtained at one time without segmenting strategy. When compared with existing ArcSAR frequency domain algorithms, the proposed algorithm is more accurate and efficient, because it has neither far range nor narrow beam antenna restrictions. The proposed method is validated by both simulation and real data. The results show that our algorithm brings the quality of image close to the time domain back-projection (BP) algorithm at a processing efficiency about two orders of magnitude better, and it has better image quality than the existing frequency domain Lee's algorithm at a comparable processing speed.
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http://dx.doi.org/10.3390/s20247027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764108PMC
December 2020

Boosting the electrocatalysis of nitrate to nitrogen with iron nanoparticles embedded in carbon microspheres.

Chem Commun (Camb) 2020 Dec 9;56(93):14685-14688. Epub 2020 Nov 9.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, P. R. China.

Human activities have increased the global nitrogen cycle imbalance, leading to serious water pollution. Inexpensive iron nanoparticles with large surface areas are in high demand in the field of environment restoration. Here, we report a hydrothermal method for the preparation of iron-carbon composites ([email protected]) with iron nanoparticles embedded in carbon microspheres. The resulting [email protected] catalyst shows a high nitrate conversion to nitrogen of 75.9% and a nitrogen selectivity of 98%. This study not only provides a simple strategy for the preparation of iron-carbon composites, but also boosts the practical application of [email protected] catalysts for water treatment.
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http://dx.doi.org/10.1039/d0cc05669cDOI Listing
December 2020

Distribution, Composition, Sources, and Potential Ecological Risks of PAHs in the Sediments of the Lanzhou Reach of the Yellow River, China.

Bull Environ Contam Toxicol 2020 Oct 22;105(4):613-619. Epub 2020 Sep 22.

Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.

The distribution, composition, sources, and potential ecological risks of polycyclic aromatic hydrocarbons (PAHs) in the sediments of the Lanzhou Reach of the Yellow River, China were investigated. The total concentration of the 18 individual PAHs (∑PAHs) in the sediments ranged from 638 to 1620 ng/g, with a mean value of 901 ng/g. The pollution level of PAHs in the sediments was low to moderate. Spatially, the distribution of PAHs in the sediments showed an increasing trend along the direction of water flow. ∑PAHs predominantly consisted of low molecular weight PAHs. The principal component analysis and isomer ratios of PAHs suggested the mixed sources of petroleum and those from the combustion of petroleum, coal, and biomass. The results showed that the PAHs in the sediments of the Lanzhou Reach of the Yellow River have a low ecological risk. However, the BaP equivalent exposure values suggested a potential cancer risk.
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http://dx.doi.org/10.1007/s00128-020-02998-4DOI Listing
October 2020

MiR-98-5p promotes ischemia/reperfusion-induced microvascular dysfunction by targeting NGF and is a potential biomarker for microvascular reperfusion.

Microcirculation 2021 01 28;28(1):e12657. Epub 2020 Sep 28.

Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, & Guangxi Key Laboratory Base of Precision Medicine in Cardio-cerebrovascular Diseases Control and Prevention & Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, Nanning, Guangxi, P.R. China.

Objective: This study examined the correlation between serum miR-98-5p levels and indices of microvascular reperfusion in patients undergoing primary percutaneous coronary intervention (pPCI) after ST-segment elevation myocardial infarction (STEMI). Additionally, we evaluated the mechanisms by which miR-98-5p promoted ischemia/reperfusion (I/R)-induced injury in both cultured cell lines and an animal model.

Methods: Circulating miR-98-5p levels were measured and compared from 171 STEMI patients undergoing pPCI, who were divided into two groups: no-reflow and reflow. The levels of miR-98-5p, nerve growth factor (NGF), and transient receptor potential vanilloid 1 (TRPV1) were analyzed in cultured human coronary endothelial cells (HCECs) exposed to hypoxia/reoxygenation (H/R). The effects of antagomir-98-5p on myocardial I/R-induced microvascular dysfunction in vivo were evaluated. Target gene expression and activity were assessed.

Results: Higher miR-98-5p levels were associated with compromised indices of microvascular reperfusion. In vitro experiments on HCECs showed that exposure to H/R significantly increased miR-98-5p levels. We identified NGF as a novel target of miR-98-5p. Further, antagomir-98-5p relieved microvascular dysfunction and enhanced the expression of NGF and TRPV1 in the rat myocardial I/R model.

Conclusions: MiR-98-5p promotes microvascular dysfunction by targeting the NGF-TRPV1 axis. Serum miR-98-5p serves as a potential biomarker for microvascular reperfusion.
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http://dx.doi.org/10.1111/micc.12657DOI Listing
January 2021

H3.3S31 phosphorylation: linking transcription elongation to stimulation responses.

Signal Transduct Target Ther 2020 08 29;5(1):176. Epub 2020 Aug 29.

Center for Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA.

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http://dx.doi.org/10.1038/s41392-020-00293-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456420PMC
August 2020

The influencing factors of Harbin (China) residents' satisfaction with municipal solid waste treatment.

Waste Manag Res 2021 Jan 13;39(1):83-92. Epub 2020 Aug 13.

Institute for Advanced Study, Tongji University, China.

China is experiencing an enormous increase in municipal household solid waste (MHSW) generation and is facing multiple problems associated with the treatment of MHSW. This paper analyses factors affecting residents' satisfaction with MHSW treatment performance. Six factors were identified by the Delphi method: (a) pick-up frequency by waste collection vehicles, (b) fund supply situation, (c) charging standard for waste treatment, (d) waste bin arrangement, (e) laws and regulations, (f) publicity and education. We examine the significance of these six influencing factors, estimating binary logistic regression models. Data for this study are derived from the survey responses of 469 households in Harbin, one of the largest cities in northeast China. The results indicate that 'pick-up frequency by waste collection vehicles' is ranked the first and most important determinant of Harbin residents' satisfaction with MHSW treatment; this is closely followed by 'publicity and education'. The third and fourth significant influencing factors, respectively, are 'fund supply situation' and 'charging standard for waste treatment'. The last two factors are 'laws and regulations' and 'waste bin arrangement'. By understanding the influence of various factors on residents' satisfaction, this study aims to help in designing an effective waste management system to reduce the cost of MHSW management, and to raise the residents' satisfaction with municipal solid waste treatment. Based on the research findings, we advocate that establishing a reasonable waste transport (pick-up) system as well as strengthening publicity and education of waste management are key to improving residents' satisfaction with the MHSW treatment performance.
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http://dx.doi.org/10.1177/0734242X20947158DOI Listing
January 2021

Prenatal evaluation and postnatal outcomes of fetal ovarian cysts.

Prenat Diagn 2020 09 17;40(10):1258-1264. Epub 2020 Jun 17.

Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

To evaluate the natural history and outcome of cases of fetal ovarian cyst under conservative prenatal treatment. A retrospective cohort study included patients diagnosed with fetal ovarian cysts was conducted between January 2008 to December 2016. Data including clinical data, sonographic feature and postnatal outcomes were obtained. One hundred and two cases were included for statistical analysis. The rate of spontaneous resolution was significantly higher among cases with simple than complex cysts (70/92 or 76.1% vs 2/10 or 20%, P < .01) and for cysts <4 cm than cysts ≥4 cm (50/56 or 89.3% vs 22/46 or 47.8%, P < .01). Ovarian torsion was confirmed in 5/102 (4.9%) cases; neither prenatal characteristics of cysts (complex: 2/10 or 20% vs simple: 3/92 or 3.3%, P = .07), nor their size (≥40 mm: 4/46 or 8.7% vs < 40 mm: 1/56 or 1.8%, P = .17) was predictive for ovarian torsion. 25/102 (24.5%) of cysts change in size or sonographic characteristics prenatally. Half of the complex cysts at the last prenatal scan are not ovarian in origin. 98/102 neonates (96.1%) were able to preserve both ovaries. Spontaneous resolution of ovarian cysts is predicted by cyst size and characteristics, whereas likelihood of torsion cannot be predicted.
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http://dx.doi.org/10.1002/pd.5754DOI Listing
September 2020

Association Between Uric Acid and N-Terminal Pro-B-Type Natriuretic Peptide in Patients With Unstable Angina Pectoris.

Am J Med Sci 2020 07 10;360(1):64-71. Epub 2020 Apr 10.

Department of Geriatric Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of China. Electronic address:

Background: The association between uric acid and N-Terminal Pro-B-Type Natriuretic Peptide (NT-proBNP) in patients with unstable angina pectoris (UAP) is unclear.

Methods: We recruited 260 patients with UAP admitted to the first affiliated Hospital of Guangxi Medical University from February 2018 to August 2018. According to the level of uric acid, patients were divided into 4 groups (Q1 = 48.00-305.00 μmol/L; Q2 = 310.00-405.00 μmol/L; Q3 = 408.00-513.00 μmol/L; Q4 = 514.00-4330.00 μmol/L). The differences of NT-proBNP between groups and the relationship with cardiac function were compared.

Results: The average age of the 260 patients enrolled was 75.04 years. The NT-proBNP of the 4 groups showed an increasing trend, and there were significant differences between the 4 groups (<0.001). On the other hand, with the increase of cardiac function (New York Heart Association), the levels of NT-proBNP and uric acid also showed an upward trend (all P < 0.05). Pearson correlation analysis showed that there was a positive correlation between uric acid log10 transform and NT-proBNP log10 transform (r = 0.272, P < 0.001). After adjusting the potential confounding factors, elevated uric acid was still significantly related to the increase of NT-proBNP (Q2 versus [vs.] Q1: OR = 469.64, 95%CI -1396.77 to 2336.05; Q3 vs. Q1: OR = 1166.53, 95%CI -726.12 to 3059.18; Q4 vs. Q1: OR = 3204.78, 95%CI 1240.86-5168.70). In subgroup analysis, the relationship between uric acid and NT-proBNP was significant in males, but no difference was observed in females.

Conclusions: In male patients with UAP, elevated uric acid is related to the increase of NT-proBNP, but this phenomenon is not obvious in female patients.
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http://dx.doi.org/10.1016/j.amjms.2020.04.004DOI Listing
July 2020

Estimation and correction of vibration-induced range cell migration for FMCW synthetic aperture ladar.

Appl Opt 2020 Mar;59(9):2874-2882

Synthetic aperture ladar (SAL) is a newly developed imaging device for remote sensing application. Owing to its short wavelength (3-5 orders of magnitude shorter than radar), SAL is very sensitive to platform vibration. For frequency-modulated continuous-wave SAL (FMCW-SAL), the platform vibration induces an additional range cell migration (RCM) to the SAL image. The vibration-induced RCM (VI-RCM) deteriorates the image quality. The VI-RCM is a unique problem for the FMCW-SAL imaging. To address this problem, a raw-data-driven method is proposed to correct the VI-RCM in this paper. First, the signal model was developed to show the VI-RCM in FMCW-SAL echo. Then, based on the model, the differential phase function (DPF) is constructed for the adjacent range profiles. The DPF is a single-frequency signal with its frequency being proportional to the relative range shift between the adjacent range profiles. Based on the DPF, the relative range shift is estimated. After the estimation of all the relative range shifts, the VI-RCM is calculated and corrected. Experiments are performed. The simulated experiment demonstrated the feasibility, accuracy, and efficiency of the proposed method, and the real data processing result verified the effectiveness of the proposed method for FMCW-SAL in practical applications.
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http://dx.doi.org/10.1364/AO.380339DOI Listing
March 2020

miR-338-5p Targets Epidermal Growth Factor-Containing Fibulin-Like Extracellular Matrix Protein 1 to Inhibit the Growth and Invasion of Trophoblast Cells in Selective Intrauterine Growth Restriction.

Reprod Sci 2020 06 13;27(6):1357-1364. Epub 2020 Feb 13.

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, People's Republic of China.

Selective intrauterine growth restriction (sIUGR) is a disorder of monochorionic (MC) twin pregnancies. However, the underlying mechanism remains largely unknown. Trophoblast cells are the major component of the placenta. Dysfunction of trophoblast cells is associated with placental dysfunction. Our previous study identified miR-338-5p is downregulated in placenta tissues sharing larger twins of sIUGR. In the present study, we aimed to investigate the role of miR-338-5p in trophoblast cells and explored its target. Our results further indicated that miR-338-5p was downregulated in placental tissues supporting larger twins of sIUGR, whereas epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) was upregulated. Moreover, miR-338-5p overexpression suppressed the growth and invasion of trophoblast cells. Importantly, results from luciferase reporter assay demonstrated that miR-338-5p bound on the 3'-UTR of EFEMP1. miR-338-5p suppressed the growth and invasion of trophoblast cells via targeting EFEMP1. Further, miR-338-5p/EFEMP1 might disrupt the function of trophoblast cells via inhibiting the phosphorylation of AKT.
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http://dx.doi.org/10.1007/s43032-020-00160-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190678PMC
June 2020

Association between mid-trimester cervical length and risk of spontaneous preterm birth is modified by a prior cervical excisional procedure.

Int J Clin Exp Pathol 2019 1;12(6):2249-2256. Epub 2019 Jun 1.

Department of Obstetrics, Women's Hospital, Zhejiang University School of Medicine Hangzhou 310006, China.

To determine whether and how prior cervical excision modifies the association between a short residual mid-trimester cervical length and the risk of spontaneous preterm birth (SPB). This is a retrospective cohort study on spontaneous preterm birth (SPB) in a total of 1875 patients with and without prior cervical excisional procedure (CEP, loop electrosurgical excision procedure or cold knife cone). Adjusting for potential confounders, we conducted a logistic regression analysis, along with a test of interaction between prior history of CEP and cervical length on SPB. Among 1875 women, there were 274 patients who experienced preterm birth (33.9%). Risk of SPB is significantly associated with age, BMI, cervical length (CL), gestational week at measurement of CL by ultrasonography, multipara, history of preterm birth, fertilization (IVF) and CEP. Moreover, a test of interaction indicated a close association between risk of SPB and CL, which was modified by history of prior CEP. We identified a CL cutoff value of 2.8 cm at which the risk of SPB in patients with prior CEP was identical to the risk in patients without prior CEP. Given the same CLs which were less than 2.8 cm, prior CEP patients presented a higher risk of preterm birth than non-CEP ones. In contrast, given the same CLs which were more than 2.8 cm, prior CEP patients presented lower risk of preterm birth than non-CEP ones. Association between mid-trimester cervical length and risk of SPB was modified by a prior cervical excisional procedure. In patients with prior CEP compared to those without, there was an increased risk of SPB at CL < 2.8 cm and a decreased risk of SPB at CL > 2.8 cm.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949615PMC
June 2019

[Fetal anteroposterior renal pelvic diameter for predicting antenatal hydronephrosis requiring postnatal surgery].

Zhejiang Da Xue Xue Bao Yi Xue Ban 2019 Jul;48(5):493-498

The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou 310052, China.

Objective: To assess the value of fetal anteroposterior renal pelvic diameter (APD) in predicting antenatal hydronephrosis requiring surgical treatment after birth.

Methods: A total of 525 cases of antenatal hydronephrosis detected by prenatal ultrasonography (ultrasound index APD ≥ 4 mm in the second trimester and APD ≥ 7 mm in the third trimester) in Zhejiang Prenatal Diagnosis Center from June 2007 to June 2018 were retrospectively analyzed. ROC curve was used to analyze the relationship between these ultrasound indicators and the requirement for surgical treatment after birth.

Results: There were 162 cases (30.9%) diagnosed in the second trimester and 363 cases (69.1%) diagnosed in the third trimester; 131 cases were diagnosed pathologically after birth, of which 121 finally underwent surgical treatment. The area under ROC curve (AUC) of APD in middle pregnancy for prediction of requiring surgery 1-12 years after birth was 0.910; the cut-off value of APD was 8.45 mm with a sensitivity of 97.1%, specificity of 70.9%, positive predictive value (PPV) of 47.9%, and negative predictive value (NPV) of 98.9%. The AUC of APD in late pregnancy for prediction of requiring surgery 1-12 years after birth was 0.800; the cut-off value of APD was 12.25 mm with a sensitivity of 66.7%, specificity of 81.2%, PPV of 51.7%, and NPV of 89.1%.

Conclusions: APD in pregnancy can be used to predict whether the fetus with hydronephrosis needs surgical treatment after birth, and the prediction value of APD in the middle pregnancy is better.
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July 2019

Impaired cell fate through gain-of-function mutations in a chromatin reader.

Nature 2020 01 18;577(7788):121-126. Epub 2019 Dec 18.

Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY, USA.

Modifications of histone proteins have essential roles in normal development and human disease. Recognition of modified histones by 'reader' proteins is a key mechanism that mediates the function of histone modifications, but how the dysregulation of these readers might contribute to disease remains poorly understood. We previously identified the ENL protein as a reader of histone acetylation via its YEATS domain, linking it to the expression of cancer-driving genes in acute leukaemia. Recurrent hotspot mutations have been found in the ENL YEATS domain in Wilms tumour, the most common type of paediatric kidney cancer. Here we show, using human and mouse cells, that these mutations impair cell-fate regulation by conferring gain-of-function in chromatin recruitment and transcriptional control. ENL mutants induce gene-expression changes that favour a premalignant cell fate, and, in an assay for nephrogenesis using murine cells, result in undifferentiated structures resembling those observed in human Wilms tumour. Mechanistically, although bound to largely similar genomic loci as the wild-type protein, ENL mutants exhibit increased occupancy at a subset of targets, leading to a marked increase in the recruitment and activity of transcription elongation machinery that enforces active transcription from target loci. Furthermore, ectopically expressed ENL mutants exhibit greater self-association and form discrete and dynamic nuclear puncta that are characteristic of biomolecular hubs consisting of local high concentrations of regulatory factors. Such mutation-driven ENL self-association is functionally linked to enhanced chromatin occupancy and gene activation. Collectively, our findings show that hotspot mutations in a chromatin-reader domain drive self-reinforced recruitment, derailing normal cell-fate control during development and leading to an oncogenic outcome.
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http://dx.doi.org/10.1038/s41586-019-1842-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061414PMC
January 2020

Application of Genome-Wide Association Studies in Coronary Artery Disease.

Curr Pharm Des 2019 ;25(40):4274-4286

Department of Population Health Science, Duke University School of Medicine, Durham, North Carolina, NC27708, United States.

Coronary artery disease (CAD) is a complex disease caused by the combination of environmental and genetic factors. It is one of the leading causes of death and disability in the world. Much research has been focussed on CAD genetic mechanism. In recent years, genome-wide association study (GWAS) has developed rapidly around the world. Medical researchers around the world have successfully discovered a series of CAD genetic susceptibility genes or susceptible loci using medical research strategies, leading CAD research toward a new stage. This paper briefly summarizes the important progress made by GWAS for CAD in the world in recent years, and then analyzes the challenges faced by GWAS at this stage and the development trend of future research, to promote the transformation of genetic research results into clinical practice and provide guidance for further exploration of the genetic mechanism of CAD.
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http://dx.doi.org/10.2174/1381612825666191105125148DOI Listing
June 2020

Disparity Management Outcomes for Patients with Severe Mental Disorders in Sichuan Province: A Preliminary Evaluation from the National Surveillance Dataset.

Biomed Environ Sci 2019 Sep;32(9):710-714

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan, China; West China Research Centre for Rural Health Development, Sichuan University, Chengdu 610041, Sichuan, China; Swinburne University of Technology, Victoria, Australia.

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http://dx.doi.org/10.3967/bes2019.090DOI Listing
September 2019

Percutaneous contrast-enhanced ultrasound for localization and diagnosis of sentinel lymph node in early breast cancer.

Sci Rep 2019 09 19;9(1):13545. Epub 2019 Sep 19.

Department of Ultrasound, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, China.

This study assessed the efficacy of percutaneous contrast-enhanced ultrasound (CEUS) in localization sentinel lymph node (SLNs) for biopsy and diagnosis of metastatic SLNs in patients with early breast cancer. From January to November 2017, seventy-five patients with early breast cancer confirmed by pathology were enrolled in this study. CEUS was performed after subdermal injection of ultrasound contrast agent (SonoVue, 2.0 ml in total dose) around the areola on the ipsilateral side of the breast. The contrast-enhanced lymphatic vessels and associated SLNs were observed and traced in real time. The lymphatic vessels and SLN were mapped and labeled on the skin surface. Sentinel lymph node biopsy (SLNB) was performed after injection of 2.0 ml methylene blue at same injection site of SonoVue. The accuracy of percutaneous CEUS localization of SLNs was determined compared to blue dye injection technique. The pathological results under blue dye guided biopsy were used as the reference standard to calculate the sensitivity and specificity of CEUS for the diagnosis of SLNs. A total of 163 SLNs obtained through SLNB following methylene blue tracing in 75 patients. There were 116 SLNs identified by percutaneous CEUS. The difference of detection rates between blue dye and CEUS was statistically significant (Z = -2.651, P = 0.008). The identification rate of SLNs by CEUS was 71.17% (116/163). The accuracy of percutaneous CEUS localization of axillary SLNs was 94.67% (71/75) compared to blue dye-guided biopsy. Among the 116 SLNs detected by percutaneous CEUS, pathologic results showed 51 positive SLNs and 65 negative SLNs whiles CEUS findings indicated 83 positive SLNs and 33 negative SLNs. Only 50 of 83 SLNs had metastasis on pathology, while 33 were detected as false positive. The sensitivity and specificity of CEUS for the diagnosis of metastatic SLN was 98.04%(50/51) and 49.23%(32/65), respectively. Percutaneous CEUS can be used as an effective method to localize the SLNs for guiding SLNB. This method has excellent sensitivity for identifying the SLNs but lower specificity for detecting metastatic SLNs in patients with early stage breast cancer.
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http://dx.doi.org/10.1038/s41598-019-49736-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753066PMC
September 2019
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