Publications by authors named "Wen Guo"

237 Publications

Tyloxapol inhibits RANKL-stimulated osteoclastogenesis and ovariectomized-induced bone loss by restraining NF-κB and MAPK activation.

J Orthop Translat 2021 May 10;28:148-158. Epub 2021 Apr 10.

Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.

Objective: Tyloxapol is a non-ionic surfactant with diverse pharmacological effects including anti-inflammatory, anti-malignant tumor and antioxidant activities. However, the effect of tyloxapol on osteoclastogenesis has not been elucidated. In this study, we intended to clarify the effect of tyloxapol on RANKL-stimulated osteoclastogenesis and the molecular mechanism both ex vivo and in vivo.

Methods: In vitro osteoclastogenesis assay was performed in BMMs and Raw 264.7 cells. The mature osteoclasts were visualized by TRAP staining. The osteoblsats were visualized by alkaline phosphatase (ALP) staining and Von Kossa staining. To assess whether tyloxapol inhibited the function of mature osteoclasts, F-actin belts and pit formation assays were carried out in BMMs. To evaluate the effect of tyloxapol on post-menopausal osteoporosis, the OVX mouse model were utilized. The bone tissue TRAP staining was used to evaluate the osteoclast activity in vivo. The von kossa staining and micro computed tomography were used to evaluate the histomorphometric parameters. The Goldner's staining was used to evaluate the osteoblast activity. The expression of osteoclastogenesis-associated markers were evaluated by Real-time PCR. The NF-κB and NFATc1 transcriptional activities were illustrated utilizing the assay of luciferase reporter. The effect of tyloxapol pretreatment on IκBa degradation and p65 phosphorylation was evaluated using Western bloting assay. The effect of tyloxapol pretreatment on p65 nuclear translocation was evaluated utilizing immunofluorescence. The effect of tyloxapol pretreatment on the phosphorylatio of ERK, p38 and JNK was examined utilizing Western bloting assay.

Results: In our research, we found that tyloxapol suppresses RANKL-stimulated osteoclastogenesis in a dose dependent manner and in the initial stage of osteoclastogenesis. Through F-actin belts and pit formation assays, we found that tyloxapol had the ability to inhibit the function of mature osteoclasts in vitro. The results of animal experiments demonstrated that tyloxapol inhibits OVX-induced bone mass loss by inhibiting the activity of osteoclasts but had a limited effect on osteoblastic differentiation and mineralization. Molecularly, we found that tyloxapol suppresses RANKL-stimulated NF-κB activation through suppressing degradation of IκBα, phosphorylation and nuclear translocation of p65. At last, MAPK signaling pathway was also suppressed by tyloxapol in dose and time-dependent manners.

Conclusion: Our research illustrated that tyloxapol was able to suppress osteoclastogenesis in vitro and ovariectomized-induced bone loss in vivo by restraining NF-κB and MAPK activation. This is pioneer research could pave the way for the development of tyloxapol as a potential therapeutic treatment for osteoporosis.

The Translational Potential Of This Article: This study explores that tyloxapol, also known as Triton WR-1339, may be a drug candidate for osteoclastogenic sicknesses like osteoporosis. Our study may also extend the clinical therapeutic spectrum of tyloxapol.
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http://dx.doi.org/10.1016/j.jot.2021.01.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063697PMC
May 2021

N6-methyladenosine demethylase FTO impairs hepatic ischemia-reperfusion injury via inhibiting Drp1-mediated mitochondrial fragmentation.

Cell Death Dis 2021 May 4;12(5):442. Epub 2021 May 4.

Department of Hepatic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Despite N6-methyladenosine (m6A) is functionally important in various biological processes, its role and the underlying regulatory mechanism in the liver remain largely unexplored. In the present study, we showed that fat mass and obesity-associated protein (FTO, an m6A demethylase) was involved in mitochondrial function during hepatic ischemia-reperfusion injury (HIRI). We found that the expression of m6A demethylase FTO was decreased during HIRI. In contrast, the level of m6A methylated RNA was enhanced. Adeno-associated virus-mediated liver-specific overexpression of FTO (AAV8-TBG-FTO) ameliorated the HIRI, repressed the elevated level of m6A methylated RNA, and alleviated liver oxidative stress and mitochondrial fragmentation in vivo and in vitro. Moreover, dynamin-related protein 1 (Drp1) was a downstream target of FTO in the progression of HIRI. FTO contributed to the hepatic protective effect via demethylating the mRNA of Drp1 and impairing the Drp1-mediated mitochondrial fragmentation. Collectively, our findings demonstrated the functional importance of FTO-dependent hepatic m6A methylation during HIRI and provided valuable insights into the therapeutic mechanisms of FTO.
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http://dx.doi.org/10.1038/s41419-021-03622-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096847PMC
May 2021

Actor and Partner Effects of Touch: Touch-Induced Stress Alleviation Is Influenced by Perceived Relationship Quality of the Couple.

Front Psychol 2021 13;12:661438. Epub 2021 Apr 13.

School of Humanities and Social Science, University of Science and Technology of China, Hefei, China.

Because of the impact of close partner's touch on psychological and physical well-being by alleviating stress, it is important to explore the influence factors that underlie the stress-alleviating effect of close partner's touch. Previous studies suggested that the stress-alleviating effect was different when individuals were touched by different persons. Specifically, the stress was reduced significantly when the individual was touched by the close partner compared with the acquaintance and the stranger. However, whether the stress-alleviating effect of touch was modulated by the close relationship quality is unknown. To examine this question, the participants ( = 61) performed a 3 (i.e., alone, partner no-touch, and partner touch) × 2 (i.e., safety and threat) within-subjects experiment. The results revealed that the stress of the participants alleviated significantly while close partners present with touch compared with without touch during facing a threat. We also found that the relationship quality of couple-members (i.e., participants perceiving the quality of alternatives and the partners' commitment level) modulated touch-induced stress alleviation. Participants perceiving the low quality of alternatives and the high partners' commitment level showed stronger touch-induced stress-alleviating effect than participants perceiving the high quality of alternatives and the low partners' commitment level. The explained variance was around 16.8% jointly for actor and partner effects. These findings provide evidence for explaining the reasons for touch-induced alleviating stress and have important implications for predicting the future effect of interactive behaviors.
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http://dx.doi.org/10.3389/fpsyg.2021.661438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076733PMC
April 2021

Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster ().

Front Physiol 2021 8;12:666842. Epub 2021 Apr 8.

School of Agriculture, Ludong University, Yantai, China.

Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster , have a different function mechanism compared with that in chordates. In this report, we identified the molecular characteristics of CgRAR to further explore the mechanism of RAR in mollusks. RT-qPCR analysis shows that CgRAR has a higher expression level in the hemocytes and gonads, indicating that CgRAR may play roles in the processes of development and metabolism. The mRNA expression level of both CgRAR and CgRXR was analyzed by RT-qPCR after injection with RA. The elevated expression of and was detected upon all--RA (ATRA) exposure. Finally, according to the results of Yeast Two-Hybrid assay and co-immunoprecipitation analysis, CgRAR and CgRXR can interact with each other through the C-terminal region. Taken together, our results suggest that CgRAR shows a higher expression level in gonads and hemocytes. ATRA exposure up-regulates the expression of CgRAR and CgRXR. Besides, CgRAR can interact with CgRXR to form a heterodimer complex.
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http://dx.doi.org/10.3389/fphys.2021.666842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060629PMC
April 2021

Serum alkaline phosphatase is associated with arterial stiffness and 10-year cardiovascular disease risk in a Chinese population.

Eur J Clin Invest 2021 Apr 13:e13560. Epub 2021 Apr 13.

Department of Health Promotion Center, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

Background: Serum alkaline phosphatase (ALP) has been recognized as a biomarker of cardiovascular disease (CVD) risk, recently. This study aimed to explore the association of ALP with arterial stiffness and 10-year CVD risk.

Methods: A total of 12 539 participants without CVD who underwent health examinations including serum ALP level were retrospectively analysed. Arterial stiffness was measured by brachial-ankle pulse wave velocity (baPWV), and 10-year CVD risk was evaluated by Framingham risk score.

Results: All participants were stratified into four groups according to the quartile of serum ALP. Participants with high ALP quartiles had higher cardiovascular parameters and baPWV, as well as an increase 10-year CVD risk. There was a dose-response relationship between serum ALP level and baPWV (OR = 1.134, 95% CI 1.103-1.165, P < .001). Logistic regression analysis showed that serum ALP was positively associated with elevated baPWV and 10-year CVD risk after adjustment for traditional CVD risk factors in both women and men. In receiver operating characteristic (ROC) curve analysis, the optimal cut-off point of serum ALP for elevated baPWV was 84U/L and the area under the ROC curve (AUROC) was 0.740 (95% CI 0.726-0.754, P < .001), with 71.2% and 63.4% sensitivity and specificity, respectively, in women. The AUROC of serum ALP in women was larger than that in men [0.575 (95% CI 0.559-0.590), P < .001].

Conclusions: Serum ALP is independently associated with arterial stiffness and 10-year CVD risk. Our results imply that serum ALP may be a promising marker to identify an increased risk for subclinical atherosclerosis in women needing further evaluation.
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http://dx.doi.org/10.1111/eci.13560DOI Listing
April 2021

The diagnostic performance of the Gynecologic Imaging Reporting and Data System (GI-RADS) in adnexal masses.

Ann Transl Med 2021 Mar;9(5):398

Department of Oncology, The General Hospital of Western Theater Command, Chengdu, China.

Background: Adnexal masses, mostly benign, are common in the female genital system. However, adnexal masses are the leading cause of death among women with gynecologic cancer. Ultrasound is a common imaging method for diagnosing adnexal masses. Gynecologic Imaging Reporting and Data System (GI-RADS) is a useful diagnostic tool based on objective ultrasound features to diagnose the malignancy of the female genital system. Therefore, we conducted a meta-analysis to evaluate the ability of GI-RADS to differentiate adnexal masses.

Methods: Published articles were searched in PubMed, Medline, and Embase from 1990 to February 2020. Pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio, and area under the curve (AUC) were estimated via the extracted data from the selected studies.

Results: Ten studies and 2,474 patients were included in this meta-analysis. The pooled sensitivity of selected studies was 0.95 [95% confidence intervals (CI): 0.94-0.97], and the pooled specificity was 0.86 (95% CI: 0.84-0.88). The pooled NLR and PLR were 0.06 (95% CI: 0.04-0.10), and 8.30 (95% CI: 4.93-13.97), respectively. Moreover, the pooled diagnostic odds ratio for GI-RADS was 174.59 (95% CI: 76.70-397.42), and the AUC was 0.9806.

Conclusions: This research indicates that GI-RADS might be a valuable tool to distinguish malignancies from adnexal masses.
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http://dx.doi.org/10.21037/atm-20-5170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033324PMC
March 2021

Triglyceride Glucose Index Is Associated With Arterial Stiffness and 10-Year Cardiovascular Disease Risk in a Chinese Population.

Front Cardiovasc Med 2021 19;8:585776. Epub 2021 Mar 19.

Department of Health Promotion Center, The First Affiliated Hospital With Nanjing Medical University, Nanjing, China.

Insulin resistance (IR) is a significant risk factor for cardiovascular disease (CVD). In this study, the association of the triglyceride glucose (TyG) index, a simple surrogate marker of IR, with arterial stiffness and 10-year CVD risk was evaluated. A total of 13,706 participants were enrolled. Anthropometric and cardiovascular risk factors were determined in all participants, while serum insulin levels were only measured in 955 participants. Arterial stiffness was measured through brachial-ankle pulse wave velocity (baPWV), and 10-year CVD risk was evaluated using the Framingham risk score. All participants were classified into four groups according to the quartile of the TyG index. BaPWV and the percentage of participants in the 10-year CVD risk categories significantly increased with increasing quartiles of the TyG index. Logistic regression analysis showed that the TyG index was independently associated with a high baPWV and 10-year CVD risk after adjusting for traditional CVD risk factors. The area under the receiver operating characteristics curve (AUROC) of the TyG index for predicting a high baPWV was 0.708 (95% 0.693-0.722, < 0.001) in women, higher than that in men. However, the association of the homeostatic model assessment of IR (HOMA-IR) with a high baPWV and the 10-year CVD risk was absent when adjusting for multiple risk factors in 955 participants. The TyG index is independently associated with arterial stiffness and 10-year CVD risk.
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http://dx.doi.org/10.3389/fcvm.2021.585776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017152PMC
March 2021

Single-shot wavefront sensing with deep neural networks for free-space optical communications.

Opt Express 2021 Feb;29(3):3465-3478

Applying deep neural networks in image-based wavefront sensing allows for the non-iterative regression of the aberrated phase in real time. In view of the nonlinear mapping from phase to intensity, it is common to utilize two focal plane images in the manner of phase diversity, while algorithms based on only one focal plane image generally yield less accurate estimations. In this paper, we demonstrate that by exploiting a single image of the pupil plane intensity pattern, it is possible to retrieve the wavefront with high accuracy. In the context of free-space optical communications (FSOC), a compact dataset, in which considerable low-order aberrations exist, is generated to train the EfficientNet which learns to regress the Zernike polynomial coefficients from the intensity frame. The performance of ResNet-50 and Inception-V3 are also tested in the same task, which ended up outperformed by EfficientNet by a large margin. To validate the proposed method, the models are fine-tuned and tested with experimental data collected in an adaptive optics platform.
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http://dx.doi.org/10.1364/OE.412929DOI Listing
February 2021

Manganese salts function as potent adjuvants.

Cell Mol Immunol 2021 May 25;18(5):1222-1234. Epub 2021 Mar 25.

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking University, Beijing, China.

Aluminum-containing adjuvants have been used for nearly 100 years to enhance immune responses in billions of doses of vaccines. To date, only a few adjuvants have been approved for use in humans, among which aluminum-containing adjuvants are the only ones widely used. However, the medical need for potent and safe adjuvants is currently continuously increasing, especially those triggering cellular immune responses for cytotoxic T lymphocyte activation, which are urgently needed for the development of efficient virus and cancer vaccines. Manganese is an essential micronutrient required for diverse biological activities, but its functions in immunity remain undefined. We previously reported that Mn is important in the host defense against cytosolic dsDNA by facilitating cGAS-STING activation and that Mn alone directly activates cGAS independent of dsDNA, leading to an unconventional catalytic synthesis of 2'3'-cGAMP. Herein, we found that Mn strongly promoted immune responses by facilitating antigen uptake, presentation, and germinal center formation via both cGAS-STING and NLRP3 activation. Accordingly, a colloidal manganese salt (Mn jelly, MnJ) was formulated to act not only as an immune potentiator but also as a delivery system to stimulate humoral and cellular immune responses, inducing antibody production and CD4/CD8 T-cell proliferation and activation by either intramuscular or intranasal immunization. When administered intranasally, MnJ also worked as a mucosal adjuvant, inducing high levels of secretory IgA. MnJ showed good adjuvant effects for all tested antigens, including T cell-dependent and T cell-independent antigens, such as bacterial capsular polysaccharides, thus indicating that it is a promising adjuvant candidate.
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http://dx.doi.org/10.1038/s41423-021-00669-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093200PMC
May 2021

Downregulation of miR-497-5p prevents liver ischemia-reperfusion injury in association with MED1/TIMP-2 axis and the NF-κB pathway.

FASEB J 2021 Apr;35(4):e21180

Department of Critical Care Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, P. R. China.

Liver ischemia-reperfusion (I/R) injury is a common clinical pathological phenomenon, which is accompanied by the occurrence in liver transplantation. However, the underlying mechanism is not yet fully understood. MicroRNAs (miRNAs) play an important role in liver I/R injury. Therefore, the study of miRNAs function will contribute a new biological marker diagnosis of liver I/R injury. This study aims to evaluate effects of miR-497-5p in liver I/R injury in mice. The related regulatory factors of miR-497-5p in liver I/R injury were predicted by bioinformatics analysis. Vascular occlusion was performed to establish the liver I/R injury animal models. Hypoxia/reoxygenation (H/R) was performed to establish the in vitro models. Hematoxylin-eosin (HE) staining was conducted to assess liver injury. The inflammatory factors were evaluated by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was adopted to assess the cell apoptosis. The expression of miR-497b-5p was increased in liver I/R injury. Knockdown of miR-497b-5p inhibited the production of inflammatory factors and cell apoptosis. Overexpression of mediator complex subunit 1 (MED1) and tissue inhibitor of metalloproteinase 2 (TIMP2) inhibited cell apoptosis to alleviate liver I/R injury. miR-497b-5p could activate the nuclear factor kappa-B (NF-κB) pathway by inhibiting the MED1/TIMP-2 axis to promote liver I/R injury. This study may provide a new strategy for the treatment of liver I/R injury.
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http://dx.doi.org/10.1096/fj.202001029RDOI Listing
April 2021

CT based automatic clinical target volume delineation using a dense-fully connected convolution network for cervical Cancer radiation therapy.

BMC Cancer 2021 Mar 8;21(1):243. Epub 2021 Mar 8.

College of Electrical and Information Engineering, Hunan University, Changsha, 410082, China.

Background: It is very important to accurately delineate the CTV on the patient's three-dimensional CT image in the radiotherapy process. Limited to the scarcity of clinical samples and the difficulty of automatic delineation, the research of automatic delineation of cervical cancer CTV based on CT images for new patients is slow. This study aimed to assess the value of Dense-Fully Connected Convolution Network (Dense V-Net) in predicting Clinical Target Volume (CTV) pre-delineation in cervical cancer patients for radiotherapy.

Methods: In this study, we used Dense V-Net, a dense and fully connected convolutional network with suitable feature learning in small samples to automatically pre-delineate the CTV of cervical cancer patients based on computed tomography (CT) images and then we assessed the outcome. The CT data of 133 patients with stage IB and IIA postoperative cervical cancer with a comparable delineation scope was enrolled in this study. One hundred and thirteen patients were randomly designated as the training set to adjust the model parameters. Twenty cases were used as the test set to assess the network performance. The 8 most representative parameters were also used to assess the pre-sketching accuracy from 3 aspects: sketching similarity, sketching offset, and sketching volume difference.

Results: The results presented that the DSC, DC/mm, HD/cm, MAD/mm, ∆V, SI, IncI and JD of CTV were 0.82 ± 0.03, 4.28 ± 2.35, 1.86 ± 0.48, 2.52 ± 0.40, 0.09 ± 0.05, 0.84 ± 0.04, 0.80 ± 0.05, and 0.30 ± 0.04, respectively, and the results were greater than those with a single network.

Conclusions: Dense V-Net can correctly predict CTV pre-delineation of cervical cancer patients and can be applied in clinical practice after completing simple modifications.
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http://dx.doi.org/10.1186/s12885-020-07595-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938586PMC
March 2021

Teaching application of hand-foot-combined-usage in Supracondylar Fracture of Femur.

Asian J Surg 2021 Mar 16;44(3):582-584. Epub 2021 Jan 16.

Inner Mongolia Medical University, Huhehot Xinhua Street, Inner Mongolia, 010059, China.

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http://dx.doi.org/10.1016/j.asjsur.2020.12.015DOI Listing
March 2021

Sulfurization-induced partially amorphous palladium sulfide nanosheets for highly efficient electrochemical hydrogen evolution.

Chem Commun (Camb) 2021 Feb 12;57(11):1368-1371. Epub 2021 Jan 12.

School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China.

A partially amorphous palladium sulfide was synthesized by sulfurizing crystalline palladium nanosheets facilely, which shows excellent activity and stability towards hydrogen evolution in alkaline media, even superior to the performance of the commercial Pt/C catalyst. The enhanced performance could be attributed to the amorphization transformation and the nanosheet morphology.
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http://dx.doi.org/10.1039/d0cc06693aDOI Listing
February 2021

NLRC3 Delays the Progression of AD in APP/PS1 Mice via Inhibiting PI3K Activation.

Oxid Med Cell Longev 2020 24;2020:5328031. Epub 2020 Dec 24.

Medical Science Research Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.

NLRC3 inhibits inflammatory responses. Neuroinflammation induces and accelerates the onset of Alzheimer's disease (AD). This study is aimed at investigating whether NLRC3 plays a role in neuroinflammation, A accumulation, and neuroprotection in AD mice. 12-month-old APP/PS1 transgenic and C57 mice were used for studies in vivo. In vitro, organotypic hippocampal slices were cultured. We found that the expression of NLRC3 was downregulated in the brain tissues of APP/PS1 mice. Mice in the APP/PS1 group had a significant attenuation of learning and memory ability compared to the control group, and the ability was improved in APP/PS1 + LV-NLRC3 mice. The expressions of 6E10, GFAP, Iba1, and PI3K in the hippocampus and brains of APP/PS1 mice were significantly higher than those of the control group, while the expressions of NeuN were lower than that of the control group. With the overexpression of NLRC3 in the APP/PS1 + LV-NLRC3 group, the expressions of 6e10, GFAP, Iba1, and PI3K were significantly lower, while the expression of NeuN was significantly higher compared to the APP/PS1 group. NLRC3 colocalized with NeuN. PI3K activation with 740YP increased the expression of GFAP and Iba-1 in the hippocampus with the exogenous NLRC3 protein. We conclude that NLRC3 may play an important role in the development and progression of AD. Downregulation of NLRC3 can lead to the activation of PI3K, resulting in abnormal plaque deposition, glial cell activation, and neuron loss during AD. NLRC3 delays the progression of AD in APP/PS1 mice via inhibiting PI3K activation.
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http://dx.doi.org/10.1155/2020/5328031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775163PMC
December 2020

Trends of Annual Whole-body Occupational Radiation Exposure for Industrial Practices in China (2009-2018).

Health Phys 2021 Apr;120(4):427-432

Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC & National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China.

Abstract: The national status and dose trends on the occupational exposure to ionizing radiation in industrial practices for 2009-2018 in China are presented in terms of seven occupational categories. A total of 504,538 industrial radiation workers were monitored for the period 2009-2018, with a continuous increase in the number of workers from 23,789 in 2009 to 66,017 in 2018. The annual average effective doses were 0.399, 0.425, 0.392, 0.376, 0.346, 0.355, 0.312, 0.305, 0.270, and 0.230 mSv from 2009 to 2018, respectively, which were well lower than the recommended occupational dose limit of 20 mSv y-1 for radiation workers. The Mann-Kendall test result shows a statistically significant decreasing trend at a rate of 0.02 mSv y-1 in average annual effective doses (p<0.001). In addition, more than 95.4% of radiation workers in industrial practices received an average annual effective dose less than the public dose limit of 1 mSv. It was also found that the average annual effective doses in industrial radiography and well logging were significantly higher than those in five other categories (p<0.001). Based on these observations, it is still necessary to control and manage the workplace and radiation workers to control occupational exposure as low as reasonably achievable, especially for the workers engaged in these two activities.
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http://dx.doi.org/10.1097/HP.0000000000001356DOI Listing
April 2021

[Research progress on the methods for measuring xylem embolism vulnerability].

Ying Yong Sheng Tai Xue Bao 2020 Nov;31(11):3895-3905

School of Ecology and Environmental Science, Yunnan University, Kunming 650500, China.

Changes in the frequency and severity of drought events associated with climate change could affect plant growth, development, and adaptability. Hydraulic failure caused by xylem embo-lism is the main physiological consequences of drought stress. How to accurately quantify xylem embolism is particularly important for understanding plant responses to drought stress. The vulnerability of xylem to embolism is usually evaluated by constructing vulnerability curves (VCs). Several methods have been developed to construct VCs, but be inconsistent in their results. A deep understanding of the design principles of xylem embolism measurement methods and comparison of the similarities and differences of various methods in actual research are particularly important for the rational interpretation of literature results, and properly using VCs in models for predicting plant responses to water deficits. Here, we compared seven methods for constructing xylem vulnerability curves to embolism: bench dehydration, centrifugation, air injection, acoustic measurements, synchrotron and X-ray microtomography (Micro-CT), optical visualization method, and pneumatron method. We summarized current achievements and controversial viewpoints of the application of these methods in specific research. Finally, we provided prospects for measuring the vulnerability of xylem embolism and the selection of relevant methods for practical application in future studies.
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http://dx.doi.org/10.13287/j.1001-9332.202011.007DOI Listing
November 2020

Stage 2 Registered Report: How subtle linguistic cues prevent unethical behaviors.

F1000Res 2020 18;9:996. Epub 2020 Aug 18.

Faculty of Arts and Science, Kyushu University, Fukuoka, Japan.

Differences in descriptions can influence people's evaluations and behaviors. A previous study by Bryan and colleagues suggested that subtle linguistic differences in ethical reminders can differentially prevent readers' unethical behavior. The present study tried to replicate the previous finding in the Japanese context (Experiment 1); additionally, we explored the influence of unfamiliar Japanese instruction words that captured participants' attention (Experiment 2). In two online experiments, participants were asked to make 10 coin-tosses and report the number of "heads" results, which would indicate the amount of money that they could earn. In Experiment 1, we analyzed the difference in the number of "heads" results as reported by 768 participants under three conditions with different instructions ("Don't cheat" vs. "Don't be a cheater" vs. baseline as a control). In Experiment 2, we conducted an extended experiment with an additional task in which more attention was directed toward the text. In Experiment 1, we successfully replicated the results of the original experiment. The results of Experiment 2 showed no evidence that the results in Experiment 1 were influenced by attentional factors. In conclusion, the results of the present study supported the hypothesis that self-identity-related words of moral reminder curb unethical behaviors more effectively. Stage 1 report: https://doi.org/10.12688/f1000research.20183.4.
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http://dx.doi.org/10.12688/f1000research.25573.2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590892PMC
April 2021

Whole Genome Sequencing Identifies Key Genes in Spinal Schwannoma.

Front Genet 2020 30;11:507816. Epub 2020 Oct 30.

Orthopedic Oncology Center, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai, China.

Spinal schwannoma is the most common primary spinal tumor but its genomic landscape and underlying mechanism driving its initiation remain elusive. The aim of the present study was to gain further insights into the molecular mechanisms of this kind of tumor through whole genome sequencing of nine spinal schwannomas and paired blood samples. The results showed that , and were the most frequently mutated cancer-related genes. In addition, the somatic copy number alterations (CNA) was potentially associated with spinal schwannoma, among which was found to be frequently deleted in schwannoma samples. Only a few genes were located within the amplified regions. In contrast, the deleted regions in 15q15.1 and 7q36.1 contained most of these genes. With respect to tumorigenesis, had the highest variant allele frequency (VAF) than other genes, and homozygous deletion was observed in , , and . Pathway-level analysis suggested that Hippo signaling pathway may be a critical pathway controlling the initiation of spinal schwannoma. Collectively, this systematic analysis of DNA sequencing data revealed that some key genes including , , and and Hippo signaling pathway were associated with spinal schwannoma, which may help improve our understanding about the genomic landscape of spinal schwannoma.
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http://dx.doi.org/10.3389/fgene.2020.507816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661748PMC
October 2020

NATIONAL DOSE REGISTRY AND TRENDS OF OCCUPATIONAL EXPOSURE TO IONISING RADIATION IN CHINA (2009-2018).

Radiat Prot Dosimetry 2020 Dec;191(3):376-381

Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC & National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China.

The registry and analysis of occupational exposure is significant for radiation protection against ionising radiation. This paper presents the current status of the 'Chinese Registry of Radiation Workers (CRRW)' and trends as well as the distribution of occupational exposure to ionising radiation in China from 2009 to 2018. A database with total 6.6 million monitoring records from 820 000 workers in 85 000 radiation units for medical uses, industrial uses and miscellaneous uses is established by the CRRW. The Mann-Kendall test shows statistically significant decreasing trends of average annual effective dose for the total, medical uses and industrial uses (p < 0.01), by 0.035, 0.042 and 0.020 mSv/y, respectively. The average annual effective dose for the monitored workers is 0.403 mSv for the 10-y period. More than 96.1% of the monitored workers receive annual doses less than the limit for public exposure (1 mSv) in 2018. The annual average effective dose in medical uses is significantly higher than industrial uses and miscellaneous uses (p < 0.05/3 = 0.017). In 2018, the annual collective dose of 107.41 man·Sv is received by 378 428 monitored workers, with medical uses as the dominant contributor. These observations could be a result of improvements in radiation protection practices in China. However, it is still necessary to take rigorous and continuous surveillance and radiation protection measures, to keep individual dose as low as reasonably achievable, especially for those found to receive relatively higher doses in medical uses.
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http://dx.doi.org/10.1093/rpd/ncaa171DOI Listing
December 2020

A preliminary metatranscriptomic insight of eggshells conditioning on substrates metabolism during food wastes anaerobic fermentation.

Sci Total Environ 2021 Mar 27;761:143214. Epub 2020 Oct 27.

College of Environment, Hohai University, Nanjing 210098, China; State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China. Electronic address:

The anaerobic treatment of food wastes (FW) for resource recovery has been extensively studied. However, the information on the traits of functional genes and enzymes for substrates metabolisms and their associations with microbial community are little. In this study, the influences of eggshells conditioning on the substrates metabolism for volatile fatty acids production (VFAs) in the process of FW fermentation were investigated at genetic levels by using the metatranscriptomic approach. The obtained results suggested that the critical genes involved in the carbohydrate and protein metabolisms (i.e. pgmB, GPI, glsA, pyrB and etc.) were up-regulated in the eggshell-conditioned reactor, which were beneficial to the bioconversion of macromolecule organics during FW fermentation. Moreover, the functional genes related with the intermediate products metabolism (i.e. pyruvate acid, butanoate) also exhibited differential genetic expression levels, which resulted in the alteration of microbial metabolic pathways and contributed to the acetic and butyric acids accumulation. In addition, a preliminary association of microbial distribution and genetic expressions was analyzed. The distinct distribution of microbial community in different FW fermentation systems affected the corresponding microbial contribution to those genetic expression levels of metabolic enzymes involved in VFAs production. This study would provide new insights of the underlying mechanism of VFAs promotion in the eggshell-conditioned FW fermentation process from the perspectives of substrates metabolisms at genetic and functional traits.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143214DOI Listing
March 2021

Two new Cd MOFs of 1,4-bis(1H-benzimidazol-1-yl)butane and flexible dicarboxylate ligands: luminescence sensing towards Fe.

Acta Crystallogr C Struct Chem 2020 Nov 22;76(Pt 11):1024-1033. Epub 2020 Oct 22.

College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, People's Republic of China.

Two new Cd MOFs, namely, two-dimensional (2D) poly[[[μ-1,4-bis(1H-benzimidazol-1-yl)butane](μ-heptanedioato)cadmium(II)] tetrahydrate], {[Cd(CHO)(CHN)]·4HO} or {[Cd(Pim)(bbimb)]·4HO} (1), and 2D poly[diaqua[μ-1,4-bis(1H-benzimidazol-1-yl)butane](μ-decanedioato)(μ-decanedioato)dicadmium(II)], [Cd(CHO)(CHN)(HO)] or [Cd(Seb)(bbimb)(HO)] (2), have been synthesized hydrothermally based on the 1,4-bis(1H-benzimidazol-1-yl)butane (bbimb) and pimelate (Pim, heptanedioate) or sebacate (Seb, decanedioate) ligands. Both MOFs were structurally characterized by single-crystal X-ray diffraction. In 1, the Cd centres are connected by bbimb and Pim ligands to generate a 2D sql layer structure with an octameric (HO) water cluster. The 2D layers are further connected by O-H...O hydrogen bonds, resulting in a three-dimensional (3D) supramolecular structure. In 2, the Cd centres are coordinated by Seb ligands to form binuclear Cd units which are linked by bbimb and Seb ligands into a 2D hxl layer. The 2D layers are further connected by O-H...O hydrogen bonds, leading to an 8-connected 3D hex supramolecular network. IR and UV-Vis spectroscopy, thermogravimetric analysis and solid-state photoluminescence analysis were carried out on both MOFs. Luminescence sensing experiments reveal that both MOFs have good selective sensing towards Fe in aqueous solution.
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http://dx.doi.org/10.1107/S2053229620013698DOI Listing
November 2020

Allosterically Coupled Multisite Binding of Testosterone to Human Serum Albumin.

Endocrinology 2021 02;162(2)

Research Program in Men's Health: Aging and Metabolism, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Human serum albumin (HSA) acts as a carrier for testosterone, other sex hormones, fatty acids, and drugs. However, the dynamics of testosterone's binding to HSA and the structure of its binding sites remain incompletely understood. Here, we characterize the dynamics of testosterone's binding to HSA and the stoichiometry and structural location of the binding sites using 2-dimensional nuclear magnetic resonance (2D NMR), fluorescence spectroscopy, 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt partitioning, and equilibrium dialysis, complemented by molecular modeling. 2D NMR studies showed that testosterone competitively displaced 18-[13C]-oleic acid from at least 3 known fatty acid binding sites on HSA that also bind many drugs. Binding isotherms of testosterone's binding to HSA generated using fluorescence spectroscopy and equilibrium dialysis were nonlinear and the apparent dissociation constant varied with different concentrations of testosterone and HSA. The binding isotherms neither conformed to a linear binding model with 1:1 stoichiometry nor to 2 independent binding sites; the binding isotherms were most consistent with 2 or more allosterically coupled binding sites. Molecular dynamics studies revealed that testosterone's binding to fatty acid binding site 3 on HSA was associated with conformational changes at site 6, indicating that residues in in these 2 distinct binding sites are allosterically coupled. There are multiple, allosterically coupled binding sites for testosterone on HSA. Testosterone shares these binding sites on HSA with free fatty acids, which could displace testosterone from HSA under various physiological states or disease conditions, affecting its bioavailability.
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http://dx.doi.org/10.1210/endocr/bqaa199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774055PMC
February 2021

Association between Neighborhood Food Environment and Body Mass Index among Older Adults in Beijing, China: A Cross-Sectional Study.

Int J Environ Res Public Health 2020 10 20;17(20). Epub 2020 Oct 20.

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China.

Objective: To investigate the association between the neighborhood food environment and body mass index (BMI) among Chinese older adults.

Methods: A multi-stage stratified random sampling method was used to recruit participants from 12 communities in Beijing, China, in 2019. Participants ( = 1764, 1034 women) in this study were older adults aged 65 to 80. We collected the participants' basic information, measured their height and weight, and calculated their BMI. Neighborhood food environments were measured by the density of and proximity to different food outlets using the Baidu Map Application Programming Interface. Adjusted multiple linear regression was performed to estimate the association between the food environment and BMI.

Results: Participants had a mean age of 69.7 ± 4.32 years old and an average BMI of 26.3 ± 3.50 kg/m. Among the three types of stores, convenience stores had the easiest access, followed by greengrocers and supermarkets. Sit-down restaurants had the best access among different restaurants, followed by Chinese fast-food restaurants, and western fast-food restaurants had the worst access. Easier access to greengrocers (β = 0.281, < 0.001) and sit-down restaurants (β = 0.304, < 0.001) was associated with higher BMI in the 250 m buffer zone. More supermarkets were associated with higher BMI in the 500 m buffer zone (β = 0.593, < 0.001). Access to convenience stores was positively associated with BMI in the 800 m buffer zone (β = 0.057, < 0.001). Better access to Chinese fast-food restaurants was associated with higher BMI (β = 0.071, = 0.001), and better access to western fast- food restaurants was associated with lower BMI (β = -0.400, < 0.001) in the 1000 m buffer zone. There was a negative association between the nearest distance to greengrocers and BMI (β = -0.004, < 0.001).

Conclusion: Although we found some significant associations between the neighborhood food environment and obesity, the current results are not strong enough to draw specific conclusions. Policymakers will need to rely on more evidence to derive concrete policy recommendations.
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http://dx.doi.org/10.3390/ijerph17207658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589694PMC
October 2020

[Automated Pre-delineation of CTV in Patients with Cervical Cancer Using Dense V-Net].

Zhongguo Yi Liao Qi Xie Za Zhi 2020 Oct;44(5):409-414

Radiotherapy Department, First Medical Center, General Hospital of Chinese People's Liberation Army, Beijing, 100853.

We use a dense and fully connected convolutional network with good feature learning in small samples, to automatically pre-deline CTV of cervical cancer patients based on CT images and evaluate the effect. The CT data of stage IB and IIA postoperative cervical cancer with similar delineation scope were selected to be used to evaluate the pre-sketching accuracy from three aspects:sketching similarity, sketching offset and sketching volume difference. It has been proved that the 8 most representative parameters are superior to those with single network and reported internationally before. Dense V-Net can accurately predict CTV pre-delineation of cervical cancer patients, which can be used clinically after simple modification by doctors.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2020.05.007DOI Listing
October 2020

Consistency of Indices Obtained via Hip Medial Ultrasound and Magnetic Resonance Imaging in Reduction and Spica Cast Treatment for Developmental Dysplasia of the Hip.

Ultrasound Med Biol 2021 Jan 8;47(1):58-67. Epub 2020 Oct 8.

Department of Ultrasound, Beijing Jishuitan Hospital, Beijing, China. Electronic address:

Children younger than 18 mo with developmental dysplasia of the hip (DDH) were treated with reduction and spica cast. X-Ray, computed tomography or magnetic resonance imaging (MRI) was used to evaluate reduction effectiveness. This study explored the hip medial ultrasonography method and anatomic structure sonograms. Twenty-eight children with DDH were enrolled. A total of 51 hips (24 left hips /27 right hips) were measured, including 30 affected hips and 21 normal hips. Various indices, including femoral head diameter (DIA), triradiate cartilage-femoral distance (TFD), acetabular-femoral distance (AFD) and ilium-femoral distance (IFD), were measured in the acetabular median coronal plane of ultrasound and the median coronal plane of MRI. The intra-group correlation coefficients for DIA, TFD, AFD and IFD were 0.968 (95% confidence interval: 0.917-0.985), 0.959 (0.929-0 976), 0.923 (0.869-0.955) and 0.950 (0914-0.971), respectively. Hip medial ultrasound and MRI exhibited good consistency. It is feasible to use hip medial ultrasound to evaluate the reduction of DDH in infants and children after spica cast.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2020.08.010DOI Listing
January 2021

Liraglutide or insulin glargine treatments improves hepatic fat in obese patients with type 2 diabetes and nonalcoholic fatty liver disease in twenty-six weeks: A randomized placebo-controlled trial.

Diabetes Res Clin Pract 2020 Dec 6;170:108487. Epub 2020 Oct 6.

900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University, Fuzhou 365000, Fujian, China. Electronic address:

Background: Type 2 diabetes mellitus is closely related to nonalcoholic fatty liver disease(NAFLD). More and more attention has been paid to the efficacy of liraglutide in the treatment of NAFLD, but the clinical evidence is still insufficient.

Objective: The purpose of this study was to use proton magnetic resonance spectroscopy (H-MRS) assessment of metformin alone poor blood glucose control of obese patients type 2 diabetes with NAFLD, added with insulin glargine, liraglutide or placebo effect in improving the fatty liver.

Methods: This is a 26-week, single-center, prospective, randomized placebo-controlled study. From September 2016 to July 2018, 128 patients with type 2 diabetes and NAFLD were enrolled in the China joint logistics team 900 hospital. The primary endpoints were the changes in intrahepatic content of lipid (IHCL), abdominal adiposity [subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT)], from baseline to week 26 (end of treatment) and the changes in liraglutide group or insulin glargine group versus change in placebo group. Secondary endpoints included the changes in liver function (AST and ALT), glycemia (HbA1c and FPG), body weight, and BMI.

Results: A total of 96 patients with type 2 diabetes and NAFLD under inadequate glycemic control by metformin were randomized (1:1:1) to receive add-on insulin glargine, liraglutide, or placebo. After 26 weeks of treatment, compared to the placebo group, in the liraglutide and insulin glargine groups, IHCL significantly decreased from baseline to week 26 (liraglutide 26.4% ± 3.2% to 20.6% ± 3.9%, P < 0.05; insulin glargine 25.0% ± 4.3% to 22.6% ± 5.8%, P > 0.05). SAT and VAT decreased significantly in the liraglutide group and in the insulin glargine group (P < 0.05). ΔSAT and ΔVAT were greater with liraglutide than insulin glargine, they were significantly different between the two groups (ΔSAT, -36 vs. - 24.5, P < 0.05; and ΔVAT, -47 vs. - 16.6, P > 0.05). In the liraglutide group, AST, ALT, and HOMA-IR decreased significantly from baseline. There was no significant difference in glucose-lowering among the three groups. During the treatment, the safety of the three groups performed well.

Conclusion: Compared with placebo, treatment with liraglutide plus an adequate dose of metformin (2000 g/ day) for 26 weeks is more effective in reducing IHCL, SAT and VAT in patients with type 2 diabetes and NAFLD. And it has additional advantages in weight loss, waist circumference reduction and liver function improvement.
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http://dx.doi.org/10.1016/j.diabres.2020.108487DOI Listing
December 2020

Probing Molecular Interactions between Surface-Immobilized Antimicrobial Peptides and Lipopolysaccharides .

Langmuir 2020 10 9;36(41):12383-12393. Epub 2020 Oct 9.

Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, United States.

Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. Recently, a label-free immobilized antimicrobial peptide (AMP) surface plasmon resonance platform was developed to successfully distinguish LPS from multiple bacterial strains. Among the tested AMPs, SMAP29 exhibited excellent affinity with LPS and has two independent LPS-binding sites located at two termini of the peptide. In this study, sum frequency generation vibrational spectroscopy was applied to investigate molecular interactions between three LPS samples and surface-immobilized SMAP29 via the N-terminus, the C-terminus, and a middle site at the solid/liquid interface in real-time, supplemented by circular dichroism spectroscopy. It was found that the conformations and orientations of surface-immobilized SMAP29 via different sites are different when interacting with the same LPS, with different interaction kinetics. The same SMAP29 sample also has different structures and interaction kinetics while interacting with different LPS samples with different charge densities and hydrophobicities. The observed results on molecular interactions between surface-immobilized peptides and LPS can well interpret the different adsorption amounts of various LPSs on different surface-immobilized peptides.
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http://dx.doi.org/10.1021/acs.langmuir.0c02492DOI Listing
October 2020

The triglyceride-glucose index is associated with the severity of hepatic steatosis and the presence of liver fibrosis in non-alcoholic fatty liver disease: a cross-sectional study in Chinese adults.

Lipids Health Dis 2020 Oct 7;19(1):218. Epub 2020 Oct 7.

Department of Health Promotion Center, the First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.

Background: The triglyceride-glucose index (TyG) is a reliable predictor of non-alcoholic fatty liver disease (NAFLD). Its association with the severity of hepatic steatosis and liver fibrosis in NAFLD is poorly understood. This study evaluated the relationship between these factors in NAFLD.

Methods: A total of 4784 participants who underwent ultrasonography were enrolled. Anthropometric and biochemical measurements were assessed. Participants with NAFLD were diagnosed by ultrasound. The degree of hepatic steatosis and liver stiffness was evaluated with transient elastography.

Results: The TyG index was significantly correlated with the severity of hepatic steatosis and the presence of liver fibrosis in patients with NAFLD. TyG quartile values correlated with increasing prevalence of NAFLD (Q1 30.9%, Q2 53.3%, Q3 71.7%, and Q4 86.4%, P < 0.001) and with the presence of liver fibrosis (Q1 13.5%, Q2 17.6%, Q3 18.8%, and Q4 26.1%, P < 0.001). The AUROC for the TyG index to predict NAFLD was 0.761, resulting in a cut-off value of 8.7. However, the AUC value of the TyG index was 0.589 for liver fibrosis, which was insufficient to predict this condition. The adjusted odds of having hepatic steatosis or liver fibrosis were more strongly associated with TyG values compared with HOMA-IR.

Conclusion: The TyG index is positively related to the severity of hepatic steatosis and the presence of liver fibrosis in NAFLD. The index also performed better than HOMA-IR.
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http://dx.doi.org/10.1186/s12944-020-01393-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541277PMC
October 2020

Increased plasma miR-146a levels are associated with subclinical atherosclerosis in newly diagnosed type 2 diabetes mellitus.

J Diabetes Complications 2020 12 26;34(12):107725. Epub 2020 Aug 26.

Department of Health Promotion Center, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. Electronic address:

Aims: The association between circulating miR-146a and subclinical atherosclerosis in type 2 diabetes mellitus (T2DM) remains poorly understood. This study aimed to investigate the correlation between plasma miR-146a levels and subclinical atherosclerosis as measured by the carotid intima-media thickness (CIMT) and brachial-ankle pulse wave velocity (baPWV) in patients with newly diagnosed T2DM.

Methods: We studied 100 patients with newly diagnosed T2DM. Subclinical atherosclerosis was defined as a thickened CIMT (≥1.0 mm) and high baPWV defined as a value greater than the 75th percentile. Plasma miR-146a levels and metabolic parameters were measured.

Results: Patients with thickened CIMT had higher plasma miR-146a levels than those without thickened CIMT (3.36 ± 1.32 vs 1.38 ± 1.11, P < 0.001). Patients in the high baPWV group had higher plasma miR-146a levels than those in the normal baPWV group (3.43 ± 1.32 vs 1.98 ± 1.48, P < 0.001). Both CIMT (β = 0.569, P < 0.001) and baPWV (β = 0.274, P = 0.001) positively correlated with plasma miR-146a levels after adjustment for confounding factors by multiple stepwise regression. On binary logistic regression, plasma miR-146a level was an independent risk factor for thickened CIMT (OR = 3.890, 95% CI 1.415-7.698, P = 0.008) and high baPWV (OR = 1.954, 95% CI 1.256-3.040, P = 0.002) after adjustment for established cardiovascular risk factors. The area under the receiver operating characteristics curve (AUROC) of plasma miR-146a level for predicting thickened CIMT was 0.795 (95%CI 0.708-0.883, P < 0.001) and for predicting high baPWV was 0.773 (95%CI 0.679-0.867, P < 0.001).

Conclusion: Plasma miR-146a levels correlate with CIMT and baPWV and could act as a biomarker for early diagnosis and as a therapeutic target for atherosclerosis in T2DM.
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http://dx.doi.org/10.1016/j.jdiacomp.2020.107725DOI Listing
December 2020

Iron coupling with carbon fiber to stimulate biofilms formation in aerobic biological film systems for improved decentralized wastewater treatment: Performance, mechanisms and implications.

Bioresour Technol 2021 Jan 21;319:124151. Epub 2020 Sep 21.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, 1 Xikang Road, Nanjing 210098, PR China; College of Environment, Hohai University, 1 Xikang Road, Nanjing 210098, PR China. Electronic address:

Iron coupling with carbon fiber (ICF) as carriers to stimulate the biofilms formation for decentralized wastewater treatment was proposed. The typical pollutants removal was accelerated and enhanced (increased by 13.65% for chemical oxygen demand, 19.68% for ammonia nitrogen and 32.66% for phosphate) in ICF compared with the traditional carbon fiber (CF) system. Mechanism explorations indicated that the iron coupling improved the surface properties of carbon fibers and contributed to the attachment and growth of biomass significantly. The components of biomass were changed with increasing proteins proportion in ICF, which was beneficial to the biofilms formation and stability. The microbial community was altered with the enrichment of functional microorganisms (i.e. Pseudomonas and Thauera). Moreover, the microbial metabolic functions (i.e. enzymatic activities and encoding genes) involved in pollutants removal derived from decentralized wastewater were highly expressed in ICF. This work provided an effective strategy to enhance the decentralized wastewater treatment.
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http://dx.doi.org/10.1016/j.biortech.2020.124151DOI Listing
January 2021