Publications by authors named "Wen Ding"

143 Publications

Maternal, placental and neonatal outcomes after asymptomatic SARS-CoV-2 infection in the first trimester of pregnancy: A case report.

Case Rep Womens Health 2021 Jul 4;31:e00321. Epub 2021 May 4.

Division of Birth Cohort Study, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

The effects of SARS-CoV-2 infection in the first trimester on the pregnant woman and the fetus remain unclear. We describe the complete follow-up of a pregnant woman with asymptomatic SARS-CoV-2 infection in the first trimester. The woman tested positive for SARS-CoV-2 viral RNA in nasopharyngeal swabs in her seventh week of gestation and was admitted to a local hospital for treatment. Although the woman had a BMI above 28 and a total gestational weight gain of 21 kg, no pregnancy complications or severe complications related to SARS-CoV-2 were reported. An ultrasound scan identified no fetal abnormalities at 22 weeks. The pregnancy ended at term (37 weeks), and the newborn's birth weight was 3100 g. Placental insufficiency was revealed by placental histology examination but this appeared not to be related to the SARS-CoV-2 infection. In-situ hybridisation and immunohistochemical tests for SARS-CoV-2 RNA, spike protein 1, and nucleocapsid proteins were negative. However, ACE-2 was positive in samples of the placenta, umbilical cord and fetal membrane. The baby was followed up through to 10 days after birth and grew normally. Our results suggest that asymptomatic SARS-CoV-2 infection in the first trimester of pregnancy might not have significant harmful effects on the mother and the developing fetus. This finding may be of interest to the general public, midwives and general practitioners. However, large population studies are needed to confirm our findings.
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http://dx.doi.org/10.1016/j.crwh.2021.e00321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095019PMC
July 2021

PyKrev: A Python Library for the Analysis of Complex Mixture FT-MS Data.

J Am Soc Mass Spectrom 2021 May 27;32(5):1263-1267. Epub 2021 Apr 27.

EaSTCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3JF, United Kingdom.

In this paper, we present PyKrev, a Python library for the analysis of complex mixture Fourier transform mass spectrometry (FT-MS) data. PyKrev is a comprehensive suite of tools for analysis and visualization of FT-MS data after formula assignment has been performed. These comprise formula manipulation and calculation of chemical properties, intersection analysis between multiple lists of formulas, calculation of chemical diversity, assignment of compound classes to formulas, multivariate analysis, and a variety of visualization tools producing van Krevelen diagrams, class histograms, PCA score, and loading plots, biplots, scree plots, and UpSet plots. The library is showcased through analysis of hot water green tea extracts and Scotch whisky FT-ion cyclotron resonance-MS data sets. PyKrev addresses the lack of a single, cohesive toolset for researchers to perform FT-MS analysis in the Python programming environment encompassing the most recent data analysis techniques used in the field.
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http://dx.doi.org/10.1021/jasms.1c00064DOI Listing
May 2021

TriBAFF-CAR-T cells eliminate B-cell malignancies with BAFFR-expression and CD19 antigen loss.

Cancer Cell Int 2021 Apr 17;21(1):223. Epub 2021 Apr 17.

Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong Province, 510317, China.

Background: To investigate the effect of TriBAFF-CAR-T cells on hematological tumor cells.

Methods: TriBAFF-CAR-T and CD19-CAR-T cells were co-cultured with BAFFR-bearing B-cell malignancies at different effector/target ratios to evaluate the anti-tumor effects. In vivo, TriBAFF-CAR-T and CD19-CAR-T cells were intravenously injected into Raji-luciferase xenograft mice. CD19 antigens losing lymphoblasts was simulated by Raji knocking out CD19 (CD19) to investigate the effect of TriBAFF-CAR-T cells on CD19 Raji.

Results: Both TriBAFF-CAR-T and CD19-CAR-T cells significantly induced the lysis of Raji, BALL-1, and Jeko-1. Moreover, when CD19-CAR-T cells specifically caused the lysis of K562 with overexpressed CD19, the lethal effect of TriBAFF-CAR-T cells was also specific for BAFFR-bearing K562 with increasing levels of interleukin-2 and INF-γ. The TriBAFF-CAR-T have the same effect with CD19-CAR-T cells in treating Raji xenofraft mice. TriBAFF-CAR-T cells also have great effect in CD19 Raji cells.

Conclusions: In this study, we successfully constructed novel TriBAFF-CAR-T cells to eliminate BAFFR-bearing and CD19 antigen loss in hematological tumor cells.
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http://dx.doi.org/10.1186/s12935-021-01923-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052726PMC
April 2021

Effect of Bismuth Oxide Particles Size on the Thermal Excitation and Combustion Properties of Thermite Systems.

ChemistryOpen 2021 Apr;10(4):464-470

College of Field Engineering, Army Engineering University of PLA, Nanjing, 210007, China.

The influence of Bi O particles size at the sub-micron scale on the thermal excitation threshold and combustion performance of nano-thermite systems was investigated. Three formulas were designed and prepared, Al(100 nm)/Bi O (170 nm), Al(100 nm)/Bi O (370 nm) and Al(100 nm)/Bi O (740 nm). The samples were characterized and tested by SEM, XRD, and DSC techniques. Electrical ignition and combustion experiments were performed. The results showed that with the increase of the particle size of Bi O , in the case of slow linear heating, the exothermic heat decreased (1051.2 J g , 527.3 J g and 243.6 J g ) and the thermal excitation threshold temperature increased (564.52 °C, 658.1 °C and 810.9 °C). Simultaneously, the state of the thermite reaction correspondingly changed to solid-solid, liquid-solid and liquid-liquid thermite reaction. In the case of rapid heating , the increase in particle size increased the excitation current (0.561A, 0.710A and 0.837A). During the combustion process, the thermite system with the smallest Bi O particle size showed the largest combustion rate, and that with the largest particle size had the longest combustion duration.
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http://dx.doi.org/10.1002/open.202000358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028500PMC
April 2021

Mini-invasive surgical instruments in transaortic myectomy for hypertrophic obstructive cardiomyopathy: a single-center experience with 168 cases.

J Cardiothorac Surg 2021 Mar 17;16(1):25. Epub 2021 Mar 17.

Shanghai Municipal Institute for Cardiovascular Diseases, 1609 Xietu Road, Shanghai, 200032, China.

Background: Although septal myectomy is a standard strategy for managing patients with hypertrophic obstructive cardiomyopathy (HOCM) and drug-refractory symptoms, so far, only a few experienced myectomy centers exist globally. Mainly, this can be explained by the many technical challenges presented by myectomy. From our clinical experience, applying the mini-invasive surgical instruments during myectomy potentially reduces the technical difficulty. This study reports the preliminary experience regarding transaortic septal myectomy using mini-invasive surgical instruments for managing patients with HOCM and drug-refractory symptoms; also, we evaluate the early results following myectomy.

Methods: Between March 2016 and March 2019, consecutive HOCM patients who underwent isolated transaortic septal myectomy using the mini-invasive surgical instruments were enrolled in this analysis. Intraoperative, in-hospital and follow-up results were analyzed.

Results: We included 168 eligible patients (83 males, mean 56.8 ± 12.3 years). The midventricular obstruction was recorded in 7 (4.2%) patients. All patients underwent transaortic septal myectomy with a mean aortic cross-clamping time of 36.0 ± 8.1 min. During myectomy, 9 (5.4%) patients received repeat aortic cross-clamping. Surgical mortality was 0.6%. Notably, 5 (3.0%) patients developed complete atrioventricular block, they needed permanent pacemaker implantation. The median follow-up time was 6 months; however, no follow-up deaths occurred with a significant improvement in New York Heart Association functional status. We reported a sharp decrease in the maximum gradients from the preoperative value (11.6 ± 7.4 mmHg vs. 94.4 ± 22.6 mmHg, p < 0.001). The median degree of mitral regurgitation fell to 1.0 (vs. 3.0 preoperatively, p < 0.001) with a significant reduction in the proportion of moderate or more regurgitation (1.2% vs. 57.7%, p < 0.001).

Conclusions: Mini-invasive surgical instruments may be beneficial in reducing the technical challenges of transaortic septal myectomy procedure. Of note, transaortic septal myectomy using the mini-invasive surgical instruments may present with favorable results.
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http://dx.doi.org/10.1186/s13019-021-01403-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968270PMC
March 2021

Health study of 11,800 workers under occupational noise in Xinjiang.

BMC Public Health 2021 Mar 6;21(1):460. Epub 2021 Mar 6.

Occupational Disease Prevention and Treatment Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830091, Xinjiang, China.

Background: We investigated the health status of some workers exposed to occupational noise in Xinjiang, and explored the influencing factors of their health level. We aimed to determine the key protection groups of occupational noise hazards, which might provide the basis for the development of targeted noise prevention measures.

Methods: We used descriptive analysis to investigate a total of 11,800 participants who underwent occupational health examination in Xinjiang Occupational Disease Prevention Hospital.

Results: The hearing abnormality rate of noise exposure participants was 8.03%, which was higher in males than females (χ = 54.507, p < 0.05). The abnormal rate of high-frequency hearing threshold in Xinjiang minorities was lower than in Han nationality (χ = 11.780, p < 0.05), while the results of the electrocardiogram were reversed (χ = 9.128, p < 0.05). Differences in abnormal rates of blood pressure (χ = 149.734, p < 0.05), hearing (χ = 231.203, p < 0.05), and physical examination (χ = 360.609, p < 0.05) are statistically significant in different industries. The abnormal rates of blood pressure (χ = 67.416, p < 0.05) and hearing (χ = 49.535, p < 0.05) gradually decrease with the expansion of the enterprise scale. Logistic regression analysis showed that gender, nationality, age, enterprise size, and industry were closely related to pure tone audiometry examination abnormal rate.

Conclusion: Workers of male, elder, in mine and small/medium enterprises should be the key populations to prevent occupational noise hazard. It is necessary to standardize occupational health management in enterprises, which helps to improve workers' self-protection awareness and quality of life.
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http://dx.doi.org/10.1186/s12889-021-10496-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937221PMC
March 2021

Research Progress on the Interaction Between Autophagy and Energy Homeostasis in Cardiac Remodeling.

Front Pharmacol 2020 30;11:587438. Epub 2020 Nov 30.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Cardiac remodeling is a common pathological process in various heart diseases, such as cardiac hypertrophy, diabetes-associated cardiomyopathy and ischemic heart diseases. The inhibition of cardiac remodeling has been suggested to be a potential strategy for preventing heart failure. However, the mechanisms involved in cardiac remodeling are quite complicated. Recent studies have reported a close correlation between autophagy and energy homeostasis in cardiac remodeling associated with various heart diseases. In this review, we summarize the roles of autophagy and energy homeostasis in cardiac remodeling and discuss the relationship between these two processes in different conditions to identify potential targets and strategies for treating cardiac remodeling by regulating autophagy.
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http://dx.doi.org/10.3389/fphar.2020.587438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734280PMC
November 2020

iTRAQ-based proteomics reveals serum protein changes in hypertensive rats induced by a high-salt diet.

EXCLI J 2020 6;19:1496-1511. Epub 2020 Nov 6.

Department of Geriatrics Cardiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

High-salt diets may increase both hypertension and risk of cardiovascular diseases. Although high-salt diets can result in hypertension and impaired vascular function, the molecular mechanisms underlying these dysfunctions are not fully known. Thus, the aims of the present study were to identify key proteins and their signaling pathways and associated molecular mechanisms that may contribute to, as well as be potential biomarkers of, the pathogenesis of hypertension-related cardiovascular diseases. To that end, the present study identified and quantitated serum proteins that were differentially expressed in male rats fed regular chow (n = 4) and those fed a high-salt diet (n = 4) to induce hypertension. The serum was collected from both groups, and the proteins differentially expressed in the serum were identified and quantitated using isobaric tags for relative and absolute quantitation combined with liquid chromatography-tandem mass spectrometry. Of 396 identified proteins, 24 were differentially expressed between the groups: 19 proteins were significantly ( < 0.05) upregulated (> 1.2 fold change), and 5 were significantly downregulated (< 0.8 fold change). Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that these differentially expressed proteins may contribute to cardiovascular diseases via the roles they play in endothelial function, vascular remodeling, the coagulation cascade, and the complement system. In addition, phagosome processes and the integrin-associated focal adhesion signaling pathway were determined to be potential underlying molecular mechanisms. The key proteins identified in this study warrant further development as new therapeutic targets or biomarkers of cardiovascular diseases associated with high-salt diet-induced hypertension.
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http://dx.doi.org/10.17179/excli2020-2740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689244PMC
November 2020

Metal organic framework ZIF-90 modified with lactobionic acid for use in improved open tubular capillary electrochromatographic enantioseparation of five basic drugs.

Mikrochim Acta 2020 Nov 11;187(12):651. Epub 2020 Nov 11.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009, People's Republic of China.

An in situ zeolite imidazole metal organic framework-90 (ZIF-90) modified capillary was prepared via the method of solvothermal synthesis. The coating of ZIF-90 was characterized by scanning electron microscopy, energy-dispersive X-ray spectrometry, and EOF. Capillary electrochromatography-based enantioseparation of the basic drugs propranolol (PRO), metoprolol (MET), atenolol (ATE), bisoprolol (BIS), and sotalol (SOT) was performed using lactobionic acid (LA) as the chiral selector. Compared with an uncoated silica capillary, the resolutions are greatly improved (PRO 1.40 → 3.23; MET 1.07 → 3.19; ATE 1.07 → 3.15; BIS 1.16 → 3.41; SOT 1.00 → 2.79). Effects of buffer pH values, proportion of organic additives, concentration of lactobionic acid, and applied voltage were investigated. Graphical abstract Schematic presentation of the preparation of zeolitic imidazolate framework-90 (ZIF-90) modified capillary (ZIF-90@capillary) for enantioseparation of drug enantiomers. The capillary was applied to construct capillary electrochromatography system with lactobionic acid for enantioseparation of basic chiral drugs.
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http://dx.doi.org/10.1007/s00604-020-04611-1DOI Listing
November 2020

Nickel-Embedded Carbon Materials Derived from Wheat Flour for Li-Ion Storage.

Materials (Basel) 2020 Oct 16;13(20). Epub 2020 Oct 16.

School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, China.

The biomass-based carbons anode materials have drawn significant attention because of admirable electrochemical performance on account of their nontoxicity and abundance resources. Herein, a novel type of nickel-embedded carbon material (nickel@carbon) is prepared by carbonizing the dough which is synthesized by mixing wheat flour and nickel nitrate as anode material in lithium-ion batteries. In the course of the carbonization process, the wheat flour is employed as a carbon precursor, while the nickel nitrate is introduced as both a graphitization catalyst and a pore-forming agent. The in situ formed Ni nanoparticles play a crucial role in catalyzing graphitization and regulating the carbon nanocrystalline structure. Mainly owing to the graphite-like carbon microcrystalline structure and the microporosity structure, the NC-600 sample exhibits a favorable reversible capacity (700.8 mAh g at 0.1 A g after 200 cycles), good rate performance (51.3 mAh g at 20 A g), and long-cycling durability (257.25 mAh g at 1 A g after 800 cycles). Hence, this work proposes a promising inexpensive and highly sustainable biomass-based carbon anode material with superior electrochemical properties in LIBs.
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http://dx.doi.org/10.3390/ma13204611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602715PMC
October 2020

Engineering Gadolinium-Integrated Tellurium Nanorods for Theory-Oriented Photonic Hyperthermia in the NIR-II Biowindow.

Small 2020 10 28;16(42):e2003508. Epub 2020 Sep 28.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, China.

Near-infrared (NIR) light-triggered hyperthermia has exhibited promising prospects in oncology therapy due to the unique merits including minimal invasiveness, monitorable, excellent therapeutic effect, and negligible side effects. Especially, the second NIR biowindow (NIR-II, 1000-1700 nm) with less absorbance and scattering by skin tissue, and deep tissue penetration, has received extensive attention for photonic hyperthermia. Unfortunately, the dissatisfactory photothermal conversion efficiency (PCE) and cumbersome preparation process of photo-driven heat conversion nanomaterials seriously hamper the future clinical application. To combat the aforementioned challenges, high imaging performance and desired therapeutic outcome 1D nanorods are constructed based on gadolinium-integrated tellurium nanorods (Te-Gd). In this system, magnetic resonance (MR) imaging and X-ray computed tomography (CT) imaging-guided photonic hyperthermia can be easily implemented in cooperation with Te-Gd. Importantly, Te-Gd possesses high PCE (41%) in the NIR-II biowindow because the transition of the excited electron can easily occur from the valence band (VB) to the conduction band (CB) on (1 0 1) and (1 0 2) crystal planes. Furthermore, the distinctive photostability, high tumor accumulation, as well as low systemic adverse effects of Te-Gd guarantee the potential in the clinic.
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http://dx.doi.org/10.1002/smll.202003508DOI Listing
October 2020

Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation.

Oxid Med Cell Longev 2020 25;2020:2647807. Epub 2020 Aug 25.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060 RP, China.

Aims: The aim of this study was to investigate whether resveratrol (RSV) could ameliorate ischemia- and hypoxia-associated cardiomyocyte apoptosis and injury via inhibiting senescence signaling and inflammasome activation.

Materials And Methods: Mice were treated with RSV by gastric tube (320 mg/kg/day) or vehicle one week before left coronary artery ligation or sham surgery until the end of the experiments. After pressure-volume loop analysis, mouse hearts were harvested for histopathological (including PSR, TTC, TUNEL staining, immunohistochemistry, and immunofluorescence) and molecular analysis by western blotting and RT-PCR. In addition, neonatal rat cardiomyocytes (NRCMs), cardiac fibroblasts (CFs), and macrophages were isolated for experiments. . RSV treatment decreased mortality and improved cardiac hemodynamics. RSV inhibited the expression of senescence markers (p53, p16, and p19), inflammasome markers (NLRP3 and Cas1 p20), and nuclear translocation of NF-B, hence alleviating infarction area, fibrosis, and cell apoptosis. RSV also inhibited expression of interleukin- (IL-) 1, IL-6, tumor necrosis factor-, and IL-18 . In experiment, RSV prevented hypoxia-induced NRCM senescence and apoptosis. After inhibition of sirtuin 1 (Sirt1) by EX27, RSV failed to inhibit p53 acetylation and expression. Moreover, RSV could inhibit expression of NLRP3 and caspase 1 p20 in NRCMs, CFs, and macrophages, respectively, in in vitro experiments. . Our findings revealed that RSV protected against ischemia-induced mouse heart injury in vivo and hypoxia-induced NRCM injury in vitro via regulating Sirt1/p53-mediated cell senescence and inhibiting NLRP3-mediated inflammasome activation.
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http://dx.doi.org/10.1155/2020/2647807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468658PMC
May 2021

Resolvin E1 protects against doxorubicin-induced cardiotoxicity by inhibiting oxidative stress, autophagy and apoptosis by targeting AKT/mTOR signaling.

Biochem Pharmacol 2020 10 1;180:114188. Epub 2020 Aug 1.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China. Electronic address:

Doxorubicin (DOX)-induced cardiotoxicity impairs the quality of life of cancer patients during or after DOX treatment, and it is imperative to explore a novel strategy to address this problem. Resolvin E1 (RvE1) is derived from eicosapentaenoic acid (EPA), which has been reported to exert beneficial effects on DOX-induced oxidative stress in cardiomyocytes. This study was designed to investigate whether RvE1 protects against DOX-induced cardiotoxicity, and the underlying mechanism was explored. DOX (20 mg/kg, one injection, i.p.) was used to induce DOX-induced cardiotoxicity in C57BL/6 mice. At 5 days after DOX administration, the effect of RvE1 was assessed by measuring cardiac function, oxidative stress, autophagy and apoptosis in cardiac tissue. We used an AKT inhibitor and rapamycin to investigate the underlying mechanisms. Our results showed that RvE1 inhibited the DOX-induced decrease in body weight and heart weight, the reduction in left ventricular ejection fraction and fractional shortening, and the increase in lactate dehydrogenase, creatine kinase myocardial bound and cardiomyocyte vacuolization. Compared to the control group, the DOX group exhibited increased oxidative stress, autophagy and apoptosis in cardiac tissue, which were alleviated by treatment with RvE1. The AKT/mTOR signaling pathways were responsible for RvE1-mediated regulation of DOX-induced oxidative stress, autophagy and myocardial apoptosis. In conclusion, RvE1 protected against DOX-induced cardiotoxicity via the regulation of AKT/mTOR signaling.
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http://dx.doi.org/10.1016/j.bcp.2020.114188DOI Listing
October 2020

The expression of interleukin 20 increases in plasma and aortic tissues from patients with acute aortic dissection.

Clin Chim Acta 2020 Nov 28;510:373-380. Epub 2020 Jul 28.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan 430060, China. Electronic address:

Objective: Acute aortic dissection (AAD) is the most devasting cardiovascular disease associated with high mortality. The occurrence and progression of AAD can be regulated by inflammatory processes, and further understanding of this inflammatory pathogenesis may improve the treatment of AAD. Interleukin 20 (IL-20), as an inflammatory mediator, has been demonstrated to be associated with several inflammatory diseases. However, the association between IL and 20 and AAD is still unknown.

Methods: Five aortic dissection tissue samples and five control aortic tissue samples were evaluated in our study. The expression of IL-20 and its receptor subunits (IL-20Rα and IL-20Rβ) was detected by immunofluorescence staining. From January 2018 to March 2018, 70 consecutive AAD patients and 25 non-AAD (NAD) patients were enrolled in this study. Diagnosis was based on computed tomography angiography (CTA) results. Blood samples were obtained from the patients on the first day of hospitalization. Plasma IL-20, TNF-α and IL-6 concentrations were evaluated with enzyme-linked immunosorbent assay (ELISA) kits.

Results: The expression levels of IL-20 and its receptor subunits (IL-20Rα and IL-20Rβ) were increased in the sites of arterial wall dissection in the AAD patients. In a separate group, the plasma IL-20, TNF-α and IL-6 concentrations were significantly higher in the AAD patients than in the non-AAD patients. Spearman's correlation analysis showed that plasma IL-20 was positively correlated with plasma TNF-α and IL-6, D-dimer, C-reaction protein (CRP), creatinine, fasting blood glucose, systolic blood pressure (SBP), and diastolic blood pressure (DBP). Multiple linear regression revealed that IL-20, in addition to IL-6, glucose, CRP and D-dimer, was independently associated with the presence of AAD.

Conclusion: IL-20 was closely associated with the presence of AAD. IL-20 may contribute to the inflammatory pathogenesis of AAD.
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http://dx.doi.org/10.1016/j.cca.2020.07.049DOI Listing
November 2020

A ball-milling synthesis of N-graphyne with controllable nitrogen doping sites for efficient electrocatalytic oxygen evolution and supercapacitors.

Dalton Trans 2020 Aug;49(31):10958-10969

Nanomaterials and Solar Energy Conversion Lab, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India.

Low-cost and efficient multifunctional electrodes play an important part in promoting the practical application of energy conversion and storage. Herein, we report the facile synthesis of N-graphyne, with a novel structure, by one-step ball milling of CaC2 and pyrazine. The accurate doping of nitrogen atoms at the controllable sites of the molecular skeleton of γ-graphyne was achieved using the nitrogenous precursor (pyrazine) as a reactant. Various techniques were adopted for the investigation of the composition, structure, and morphology of the obtained samples. The electrochemical measurements demonstrated that N-graphyne can serve as an excellent electrode material for both electrocatalysis and supercapacitors. As an electrocatalyst, N-graphyne exhibited an overpotential of 280 mV at 100 mA cm-2 and a Tafel slope of 122 mV dec-1 for the oxygen evolution reaction with highly stable morphology and electrocatalytic performance. As a supercapacitor electrode, N-graphyne showed a maximum capacitance of 235 F g-1 at 1 A g-1, and capacitance retention of 87% after 3000 cycles. The superior electrochemical performance of N-graphyne is due to the nitrogen heteroatomic defects, large electrochemical active surface areas and fast electron migration. Our studies provide a facile synthesis of novel N-graphyne with controllable doping sites and promote its potential applications in electrocatalysis and supercapacitors.
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http://dx.doi.org/10.1039/d0dt01855dDOI Listing
August 2020

The interaction of RAAS inhibitors with COVID-19: Current progress, perspective and future.

Life Sci 2020 Sep 24;257:118142. Epub 2020 Jul 24.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China. Electronic address:

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently defined as the worst pandemic disease. SARS-CoV-2 infects human cells via the binding of its S protein to the receptor angiotensin-converting enzyme (ACE2). The use of ACEIs/ARBs (RAAS inhibitors) regulates the renin-angiotensin-aldosterone system (RAAS) and may increase ACE2 expression. Considering the large use of ACEIs/ARBs in hypertensive patients, some professional groups are concerned about whether the use of RAAS inhibitors affects the risk of SARS-CoV-2 infection or the risk of severe illness and mortality in COVID-19 patients. In this review, we summarize preclinical and clinical studies to investigate whether the use of ACEIs/ARBs increases ACE2 expression in animals or patients. We also analyzed whether the use of these drugs affects the risk of SARS-CoV-2 infection, severe illness or mortality based on recent studies. Finally, the review suggests that current evidence does not support the concerns.
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http://dx.doi.org/10.1016/j.lfs.2020.118142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377983PMC
September 2020

The Clinical Characteristics and Prognosis Factors of Mild-Moderate Patients With COVID-19 in a Mobile Cabin Hospital: A Retrospective, Single-Center Study.

Front Public Health 2020 5;8:264. Epub 2020 Jun 5.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Novel mobile cabin hospitals have been built to provide more makeshift beds for patients with COVID-19 in Wuhan. However, the characteristics of these patients needed be further described. This was a retrospective, single-center study. A total of 869 patients with confirmed COVID-19 were admitted to Wuchang Mobile Cabin Hospital in Wuhan, between February 6th, 2020 and February 20th, 2020. The final date of follow-up was March 6th, 2020. Clinical characteristics and outcome data were collected and analyzed. Of 869 patients, the median age was 51 years (IQR, 40-58 years), and 377 patients (377/869; 43.4%) were men. A total of 616 patients (616/869; 70.9%) were discharged, 95 patients (95/869; 10.9%) were transferred to the designated hospital due to worsening condition (endpoint), and 158 patients (158/869; 18.2%) were still in the hospital. The incidence of the main symptoms, including fever, cough, fatigue, muscle aches, and anorexia, decreased with time. However, there were no differences in outcome among the patients with different onset times. Generally, both patients aged 45 years or older and patients with comorbidities were more likely to reach the endpoint (transfer to designated high-level hospitals due to condition worsen). In the other model, patients with the lung CT feature (e.g., ground-glass opacity, reticular/linear, air bronchogram, or consolidation shadow) were more likely to reach the endpoint. Older age, comorbidity, special chest CT features (e.g., ground-glass opacity, reticular/linear, air bronchogram, or consolidation shadow) are associated with poor prognosis for mild-moderate patients. The initial symptoms of mild-moderate patients may become insidious, which deserves our attention.
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http://dx.doi.org/10.3389/fpubh.2020.00264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291856PMC
January 2021

Fully Elastomeric Fingerprint-Shaped Electronic Skin Based on Tunable Patterned Graphene/Silver Nanocomposites.

ACS Appl Mater Interfaces 2020 Jul 1;12(28):31725-31737. Epub 2020 Jul 1.

Key Laboratory of Advanced Manufacturing Technology of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China.

Multifunctional electronic skins (e-skins), which mimic the somatosensory system of human skin, have been widely employed in wearable devices for intelligent robotics, prosthetics, and human health monitoring. Relatively low sensitivity and severe mutual interferences of multiple stimuli detection have limited the applications of the existing e-skins. To address these challenges, inspired by the physical texture of the natural fingerprint, a novel fully elastomeric e-skin is developed herein for highly sensitive pressure and temperature sensing. A region-partition strategy is utilized to construct the multifunctional fingerprint-shaped sensing elements, where strain isolation structure of indurated film patterns are further embedded to enhance the sensitivity and effectively reduce mutual interferences between the differentiated units. The fully elastomeric graphene/silver/silicone rubber nanocomposites are synthesized with tunable properties including conductivity and sensitivity to satisfy the requirements of highly sensitive pressure and temperature sensing as well as stretchable electrodes. Remarkable progress in sensitivities for both pressure and temperature, up to 5.53 kPa in a wide range of 0.5-120 kPa and 0.42% °C in 25-60 °C, respectively, are achieved with the inappreciable mutual interferences. Further studies demonstrate the great potential of the proposed e-skin in the next-generation of wearable electronics for human-machine interfaces.
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http://dx.doi.org/10.1021/acsami.0c09653DOI Listing
July 2020

Gold nanoparticles-functionalized monolithic column for enantioseparation of eight basic chiral drugs by capillary electrochromatography.

Mikrochim Acta 2020 02 19;187(3):178. Epub 2020 Feb 19.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, Jiangsu, 210009, People's Republic of China.

Poly(glycidyl methacrylate)-co-(ethylene dimethacrylate) [poly(GMA-co-EDMA)] monoliths were prepared, and used as a support to attach gold nanoparticles (AuNP) via Au-S bond. Pepsin, acting as a chiral selector, was linked to the surface of the carboxyl-modified AuNP through a hydrochloride/N-hydroxysuccinimide coupling reaction. The material was characterized by scanning electron microscopy, energy dispersive X-ray spectrometry, transmission electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy and N adsorption-desorption isotherm. The pepsin@AuNP@poly(GMA-co-EDMA) monolith showed preferable enantioselectivity for hydroxychloroquine (HCQ), chloroquine (CHQ), hydroxyzine (HXY), labetalol (LAB), nefopam (NEF), clenbuterol (CLE), amlodipine (AML) and chlorpheniramine (CHL) in capillary electrochromatography (CEC). These racemic drugs were monitored at the maximum absorption wavelength (220 nm for HXQ, CHQ, HXY, LAB, NEF; 240 nm for AML; 215 nm for CLE, CHL). In comparison with the pepsin@poly(GMA-co-EDMA) monolith loaded with 5 nm AuNP, the pepsin@poly(GMA-co-EDMA) monolith loaded with 13 nm AuNP shows significantly enhanced enantiomeric resolution (HCQ: 0.62 → 3.45; CHQ: 0.60 → 2.11; HXY: 0.49 → 2.30; LAB: 1.03 → 2.45, 1.45 → 3.46, 0 → 0.67; NEF: 0.53 → 1.29; CLE: 0.42 → 0.56; AML: 0 → 0.83; CHL: 0.24 → 0.55). Pepsin concentration, buffer pH value, buffer concentration and applied voltage were investigated in detail with (±) HCQ and (±) HXY as model analytes. The reproducibility of intra-day, inter-day and column-to-column were explored, and found to be satisfactory. Graphical abstractSchematic presentation of the preparation of gold nanoparticles (AuNP) modified.
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http://dx.doi.org/10.1007/s00604-020-4144-8DOI Listing
February 2020

A monolithic capillary modified with a copoplymer prepared from the ionic liquid 1-vinyl-3-octylimidazolium bromide and styrene for electrochromatography of alkylbenzenes, polycyclic aromatic hydrocarbons, proteins and amino acids.

Mikrochim Acta 2019 12 18;187(1):67. Epub 2019 Dec 18.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, Jiangsu, 210009, People's Republic of China.

A monolithic capillary column was prepared by single-step copolymerization of the ionic liquid 1-vinyl-3-octylimidazolium bromide (VOI) and styrene. The VOI and styrene monomers were introduced to provide multiple interaction sites and increase hydrophobicity and aromaticity of the monolithic column, respectively. The effect of porogen ratio, monomer ratio and reaction temperature on permeability was investigated. The resulting column was characterized by scanning electron microscopy, and the results suggest that the column possesses high porosity and good homogeneity. A relatively strong anodic electroosmotic flow was generated over a wide range of pH values (pH 2.0-10.0), and this facilitates the rapid separation of analytes within 12 min. Alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), proteins and amino acids were used to evaluate the performance of the monolithic column under capillary electrochromatography mode by UV detection. Mixed-mode retention mechanisms including hydrophobic interaction, π-stacking, ion-exclusion interaction were observed. The monolithic column exhibits high column efficiency (8.72 × 10 plates∙m) and satisfying separation capability (the resolution of four alkylbenzenes: 2.54, 2.86 and 4.62, four PAHs: 2.79, 4.83 and 4.77, three proteins: 4.35 and 2.86, two amino acids: 3.34). Graphical abstractSchematic representation of capillary electrochromatography (CEC) systems with ionic liquid and styrene based organic polymer monolithic column for separation of alkylbenzenes, polycyclic aromatic hydrocarbons, proteins and amino acids.
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http://dx.doi.org/10.1007/s00604-019-3894-7DOI Listing
December 2019

A metal organic framework-functionalized monolithic column for enantioseparation of six basic chiral drugs by capillary electrochromatography.

Mikrochim Acta 2019 12 17;187(1):51. Epub 2019 Dec 17.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009, People's Republic of China.

Poly(glycidyl methacrylate)-co-(ethylene dimethacrylate) [poly(GMA-co-EDMA)] monoliths were used as a support to grow a zeolitic imidazolate framework-8 (ZIF-8) via layer-by-layer self-assembly. Pepsin, acting as as chiral selector, was covalently linked to the surface of the amino-modified ZIF-8 through the Schiff base method. The material was characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Fourier transform infrared spectroscopy and elemental analysis. The pepsin-ZIF-8-poly(GMA-co-EDMA) column was utilized to the enantioseparation of the racemic forms of hydroxychloroquine (HCQ), chloroquine (CHQ), hydroxyzine (HXY), nefopam (NEF), clenbuterol (CLE) and amlodipine (AML). In comparison with a pepsin-poly(GMA-co-EDMA) monolithic column (without self-assembled ZIF-8 nanoparticles), the resolution is strongly enhanced (HCQ: 0.34 → 2.50; CHQ: 0.45 → 1.97; HXY: 0.39 → 1.43; NEF: 0.27 → 0.81; CLE: 0 → 0.81; AML: 0.16 → 0.72). Effects of self-assembly layers of ZIF-8, pepsin concentration, buffer pH values and applied voltage were investigated with hydroxychloroquine as the model analyte. The reproducibility of run-to-run, day-to-day and column-to-column were explored, and found to be satisfactory. Graphical abstractSchematic representation of capillary electrochromatography (CEC) systems with a pepsin-zeolitic imidazolate framework-8 (ZIF-8) modified poly(glycidyl methacrylate)-co-(ethylene dimethacrylate) [poly(GMA-co-EDMA)] monolithic column as stationary phases for separation of basic racemic drugs. ZIF-8 modified column was prepared via layer-by-layer self-assembly.
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http://dx.doi.org/10.1007/s00604-019-3998-0DOI Listing
December 2019

Suppressing miR-21 activity in tumor-associated macrophages promotes an antitumor immune response.

J Clin Invest 2019 12;129(12):5518-5536

Department of Comparative Medicine.

microRNA-21 (miR-21) is the most commonly upregulated miRNA in solid tumors. This cancer-associated microRNA (oncomiR) regulates various downstream effectors associated with tumor pathogenesis during all stages of carcinogenesis. In this study, we analyzed the function of miR-21 in noncancer cells of the tumor microenvironment to further evaluate its contribution to tumor progression. We report that the expression of miR-21 in cells of the tumor immune infiltrate, and in particular in macrophages, was responsible for promoting tumor growth. Absence of miR-21 expression in tumor- associated macrophages (TAMs), caused a global rewiring of their transcriptional regulatory network that was skewed toward a proinflammatory angiostatic phenotype. This promoted an antitumoral immune response characterized by a macrophage-mediated improvement of cytotoxic T-cell responses through the induction of cytokines and chemokines, including IL-12 and C-X-C motif chemokine 10. These effects translated to a reduction in tumor neovascularization and an induction of tumor cell death that led to decreased tumor growth. Additionally, using the carrier peptide pH (low) insertion peptide, we were able to target miR-21 in TAMs, which decreased tumor growth even under conditions where miR-21 expression was deficient in cancer cells. Consequently, miR-21 inhibition in TAMs induced an angiostatic and immunostimulatory activation with potential therapeutic implications.
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http://dx.doi.org/10.1172/JCI127125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877327PMC
December 2019

Ultrasound-promoted synthesis of γ-graphyne for supercapacitor and photoelectrochemical applications.

Ultrason Sonochem 2020 Mar 29;61:104850. Epub 2019 Oct 29.

School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.

As a novel type of carbon materials, graphynes possesses the merits of high carrier mobility and large surface areas, etc. However, to date, the main research of graphynes is focused on theoretical calculation whereas few strategies have been reported for the fabrication of graphynes. In this work, a facile method named ultrasound-promoted synthesis was developed to fabricate γ-graphyne using PhBr and CaC as the raw materials. The reaction system in argon atmosphere ultrasonically vibrated for 24 h in the ultrasonic bath at a power of 180 W and frequency of 53 kHz. The structure, morphology, and component of the obtained samples were detected by X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, FT-IR spectra, scanning electron microscopy, transmission electron microscopy, and the selected area electron diffraction. The electrochemical and photoelectrochemical measurements indicate that γ-graphyne can be used as superior electrode mateirals in supercapacitor and photoelectrochemical catalysis. From the results of galvanostatic charge/discharge measurements, the γ-graphyne supercapacitor delivers a maximum specific capacitance of 81 F/g at 0.2 A/g and a capacitance retention rate of 87.5% after 5000 cycles at 3 A/g. Moreover, UV-vis light photoelectrochemical response and high carrier density are observed for γ-graphyne. It is also demonstrated that the charge-transfer resistance is low by Tafel slopes and Nyquist plots. This work puts forward a new and facile strategy for the fabrication of γ-graphyne and explores its application in the field of solar energy conversion and storage.
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http://dx.doi.org/10.1016/j.ultsonch.2019.104850DOI Listing
March 2020

Obeticholic acid prevents carbon tetrachloride-induced liver fibrosis through interaction between farnesoid X receptor and Smad3.

Int Immunopharmacol 2019 Dec 28;77:105911. Epub 2019 Oct 28.

First Affiliated Hospital, Anhui Medical University, Hefei 230032, China. Electronic address:

Liver fibrosis results from sustained liver injury and is characterized by inflammation, hepatic stellate cell (HSC) activation, extracellular matrix (ECM) accumulation and liver structure destruction. The Farnesoid-X receptor (FXR) antagonizes toxic liver injury and fibrosis, yet the mechanism in liver fibrosis remains unclear. We investigated the effects of FXR agonist obeticholic acid (OCA) on liver fibrosis in mice. Mice were injected with carbon tetrachloride (CCl) for 3 weeks or 6 weeks to induce liver fibrosis. OCA (5 mg/kg) or PBS is administered daily during CCl-treatment. At sacrifice, biochemical parameters and fibrosis were assessed. Pretreatment with OCA alleviated hepatic injury in 6 weeks group but not in 3 weeks group of CCl liver cirrhosis. At same time, pretreatment with OCA exhibit a dramatic protection of liver fibrosis in both 3 weeks group and 6 weeks group. Further experiments found that OCA pretreatment inhibited α-SMA expression and the activation of hepatic pSmad3 in 3 weeks group and 6 weeks group of CCl-induced liver cirrhosis. Moreover, OCA activated FXR nuclear translocation and increased the interaction between liver FXR and pSmad3. This led to the discovery of a novel role for FXR in regulating fibrosis through interaction with pSmad3. Our data suggest that CCl-induced liver fibrosis is protected by OCA through interaction between farnesoid X receptor and Smad3.
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http://dx.doi.org/10.1016/j.intimp.2019.105911DOI Listing
December 2019

miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42/-Catenin Signaling.

Int J Endocrinol 2019 28;2019:5219782. Epub 2019 Aug 28.

Department of Pathophysiology, Medical College, Nanchang University, 461 Bayi Road, Nanchang 330006, China.

Background: Diabetes is a progressive metabolic disease characterized by hyperglycemia. Functional impairment of islet cells can occur to varying degrees. This impairment can initially be compensated for by proliferation and metabolic changes of cells. Cell division control protein 42 (Cdc42) and the microRNA (miRNA) miR-29 have important roles in -cell proliferation and glucose-stimulated insulin secretion (GSIS), which we further explored using the mouse insulinoma cell line MIN6.

Methods: Upregulation and downregulation of miR-29a and Cdc42 were accomplished using transient transfection. miR-29a and Cdc42 expression was detected by real-time PCR and western blotting. MIN6 proliferation was detected using a cell counting kit assay. GSIS under high-glucose (20.0 mM) or basal-glucose (5.0 mM) stimulation was detected by enzyme-linked immunosorbent assay. The miR-29a binding site in the Cdc42 mRNA 3'-untranslated region (UTR) was determined using bioinformatics and luciferase reporter assays.

Results: miR-29a overexpression inhibited proliferation ( < 0.01) and GSIS under high-glucose stimulation ( < 0.01). Cdc42 overexpression promoted proliferation ( < 0.05) and GSIS under high-glucose stimulation ( < 0.05). miR-29a overexpression decreased Cdc42 expression ( < 0.01), whereas miR-29a downregulation increased Cdc42 expression ( < 0.01). The results showed that the Cdc42 mRNA 3'-UTR is a direct target of miR-29a . Additionally, Cdc42 reversed miR-29a-mediated inhibition of proliferation and GSIS ( < 0.01). Furthermore, miR-29a inhibited -catenin expression ( < 0.01), whereas Cdc42 promoted -catenin expression ( < 0.01).

Conclusion: By negatively regulating Cdc42 and the downstream molecule -catenin, miR-29a inhibits MIN6 proliferation and insulin secretion.
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http://dx.doi.org/10.1155/2019/5219782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735210PMC
August 2019

Genetic deficiency or pharmacological inhibition of miR-33 protects from kidney fibrosis.

JCI Insight 2019 11 14;4(22). Epub 2019 Nov 14.

Department of Cell Biology and Immunology, Centro de Biología Molecular "Severo Ochoa," Madrid, Spain.

Previous work has reported the important links between cellular bioenergetics and the development of chronic kidney disease, highlighting the potential for targeting metabolic functions to regulate disease progression. More recently, it has been shown that alterations in fatty acid oxidation (FAO) can have an important impact on the progression of kidney disease. In this work, we demonstrate that loss of miR-33, an important regulator of lipid metabolism, can partially prevent the repression of FAO in fibrotic kidneys and reduce lipid accumulation. These changes were associated with a dramatic reduction in the extent of fibrosis induced in 2 mouse models of kidney disease. These effects were not related to changes in circulating leukocytes because bone marrow transplants from miR-33-deficient animals did not have a similar impact on disease progression. Most important, targeted delivery of miR-33 peptide nucleic acid inhibitors to the kidney and other acidic microenvironments was accomplished using pH low insertion peptides as a carrier. This was effective at both increasing the expression of factors involved in FAO and reducing the development of fibrosis. Together, these findings suggest that miR-33 may be an attractive therapeutic target for the treatment of chronic kidney disease.
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http://dx.doi.org/10.1172/jci.insight.131102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948871PMC
November 2019

The Relationship Between Sensation Seeking and Tobacco and Alcohol Use Among Junior High School Students: The Regulatory Effect of Parental Psychological Control.

Front Psychol 2019 4;10:2022. Epub 2019 Sep 4.

School of Psychology, Shandong Normal University, Jinan, China.

The present study primarily aims to examine differences in the use of tobacco and alcohol by junior high school students under different parental control levels (including parental psychological control and parental behavioral control). It thus explores the regulatory effect of parental control on the relationship between adolescent sensation seeking and tobacco and alcohol use. A total of 1,050 junior high school students in Shandong province were surveyed using sensation-seeking scale, parental control scale, and adolescent health-related risk behavior questionnaire. As the results showed, (1) sensation seeking and gender had effects on the use of tobacco and alcohol among junior high school students; (2) parental psychological control can enhance and moderate the relationship between sensation seeking and the use of tobacco and alcohol; (3) parental behavioral control cannot regulate the relationship between sensation seeking and the use of tobacco and alcohol among junior high school students; and (4) no significant urban-rural differences were found regarding the regulatory effects of parental psychological control on sensation seeking and alcohol and tobacco use in junior high school students.
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http://dx.doi.org/10.3389/fpsyg.2019.02022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737919PMC
September 2019

[Responses of soil organic carbon and microbial community structure to different tillage patterns and straw returning for multiple years.]

Ying Yong Sheng Tai Xue Bao 2019 Sep;30(9):3183-3194

Shandong Province Key Laboratory of Soil and Water Conservation and Environmental Conservation, College of Resources and Environment, Linyi University, Linyi 276000, Shandong, China.

Soil organic carbon is essential for maintaining terrestrial ecosystem function and mitigating soil degradation. Soil microorganisms participate in soil carbon cycling. They are affected by tillage methods and straw returning. A split-plot design was adopted in this experiment. The whole-plot treatment had two tillage methods, subsoil tillage (ST) and rotary tillage (RT). The split-plot treatment included full straw returning (F) and no straw returning (0). The microbial community structure and carbon sequestration genes were assessed by Illumina sequencing technique. Soil organic carbon contents were measured during 2012-2017. The results showed that 1) subsoil tillage and straw returning significantly increased pH, microbial biomass carbon, total nitrogen, silt content, and clay content, while significantly decreased sand content; 2) during the test period (2012-2017), soil organic carbon (SOC) content under all treatments showed an increasing trend, but the increment for average SOC content under straw returning and subsoiling treatments was significantly higher than that of no straw returning and rotary tillage by 33.2 % and 30.6%, respectively; 3) Proteobacteria was the most abundant type of bacteria in the soil, followed by Acidobacteria and Gemmatadanetes; 4) STF treatment maintained high microbial diversity; 5) Excepted for soil sand content, soil pH, microbial biomass carbon, total nitrogen, silt content and clay content all caused the variation of soil microbial community structure under the STF treatment in the direction of SOC accumulation; 6) in addition to the gene abundance in the di- and oligosaccharides metabolic pathway, the gene abundance in the metabolic pathways for CO fixation, central carbohydrate metabolism, fermentation, one-carbon metabolism, organic acids, sugar alcohols and glycoside hydrolases showed that subsoil tillage was significantly higher than rotary tillage, with posi-tively correlation with soil organic carbon content. Therefore, the combination of subsoil tillage and straw returning could improve basic soil properties, affect soil microbial community structure, and increase the capacity of soil carbon fixation, thus providing a realistic basis for solving soil degradation.
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http://dx.doi.org/10.13287/j.1001-9332.201909.039DOI Listing
September 2019

An organic polymer monolith modified with an amino acid ionic liquid and graphene oxide for use in capillary electrochromatography: application to the separation of amino acids, β-blockers, and nucleotides.

Mikrochim Acta 2019 08 20;186(9):636. Epub 2019 Aug 20.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, Jiangsu, 210009, People's Republic of China.

The preparation of an organic polymer monolithic column modified with an amino acid ionic liquid and graphene oxide (AAIL-GO) and its application to capillary electrochromatography (CEC) was described. The AAIL tetramethylammonium-L-arginine was bonded to a monolithic column that was previously modified with graphene oxide by using an hydrochloride/N-hydroxysuccinimide coupling reaction. The morphology of a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was examined by scanning electron microscopy. The incorporation of AAIL and graphene oxide was detected by infrared spectroscopy and elemental analysis. The resulting monolithic column produced a strong and stable electroosmotic flow from the anode to the cathode in the pH range from 3 to 9. Compared with a column modified with AAIL or graphene oxide only, the AAIL-GO-modified column has a better separation ability for amino acids, β-blockers, and nucleotides (the resolution of three amino acids: 2.231 and 2.036, β-blockers: 2.779 and 2.470 and nucleotides: 8.345 and 3.321). Molecular modeling was applied to demonstrate the separation mechanism of small molecules which showed a good support for experimental results. Graphical abstract Schematic representation of capillary electrochromatography (CEC) systems with an amino acid ionic liquid-graphene oxide modified organic polymer monolithic column as stationary phases for separation of amino acids, β-blockers, and nucleotides.
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http://dx.doi.org/10.1007/s00604-019-3723-zDOI Listing
August 2019

Impact of Female Obesity on Cumulative Live Birth Rates in the First Complete Ovarian Stimulation Cycle.

Front Endocrinol (Lausanne) 2019 2;10:516. Epub 2019 Aug 2.

Reproductive Medicine Center, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Female overweight/obesity has been reported to be associated with compromised pregnancy outcomes in fresh embryo transfer cycles. It is unclear whether the cumulative live birth rate (CLBR) is adversely affected after all viable embryos are transferred from the first ovarian stimulation cycle. To investigate whether the CLBR was compromised in obese women. A total of 9,772 young women underwent their first IVF/ICSI cycles from January 2012 to October 2017. Pregnancy outcomes were compared according to female BMI. Among 1,671 women with polycystic ovary syndrome (PCOS), those with a BMI ≥ 28 kg/m had a lower cumulative clinical pregnancy rate (CCPR) and CLBR during the first complete ovarian stimulation cycle. Additionally, the pregnancy loss rate was increased in this group, although the difference was not significant. Among the 8,101 women without PCOS, the CCPR and CLBR of obese patients was also significantly decreased, and this group also showed increased pregnancy loss rates. Moreover, overweight women also had a decreased CLBR. Female obesity adversely affected the CLBR after utilizing the viable embryos from first oocytes retrieval.
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http://dx.doi.org/10.3389/fendo.2019.00516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687867PMC
August 2019