Publications by authors named "Weiyi Zhang"

90 Publications

Exosomes From Packed Red Cells Induce Human Mast Cell Activation and the Production of Multiple Inflammatory Mediators.

Front Immunol 2021 6;12:677905. Epub 2021 May 6.

Department of Anesthesiology, West China Hospital, Sichuan University & The Research Unit of West China (2018RU012), Chinese Academy of Medical Science, Chengdu, China.

Most blood transfusion-related adverse reactions involve the immunologic responses of recipients to exogenous blood components. Extracellular vesicles isolated from packed red cells can affect the recipient's immune system. Mast cells are traditionally known as effector cells for allergic transfusion reactions. However, growing evidence supports the notion that activated mast cells might disturb host innate immunologic responses. Exosomes are a type of extracellular vesicle. To determine the effect of exosomes on mast cells, we enriched exosomes derived from volunteer plasma (EXs-nor) and packed red cells (EXs-RBCs) using ultracentrifugation and incubated them with a human mast cell line (HMC-1). We found that EXs-RBC exposure increased the expression of tryptase-1 and prostaglandin D2, the production of multiple inflammatory mediators, and the levels of Toll-like receptor-3 (TLR-3) and phospho-mitogen-activated protein kinase (MAPK) in HMC-1 cells. MAPK inhibitors (SB203580, PD98059, and SP600125) and a TLR-3/dsRNA complex inhibitor reduced the EXs-RBC-stimulated production of inflammatory mediators in HMC-1 cells, whereas the TLR-3 agonist [poly (A:U)] elevated the production of these mediators. These results indicate that EXs-RBCs activate HMC-1 cells and elicit the production of multiple inflammatory mediators, partly the TLR-3 and MAPK pathways. Mast cells activated by EXs-RBCs exhibit complex inflammatory properties and might play a potential role in transfusion-related adverse reactions.
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http://dx.doi.org/10.3389/fimmu.2021.677905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135094PMC
May 2021

Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.

Nat Commun 2021 05 17;12(1):2863. Epub 2021 May 17.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages.
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http://dx.doi.org/10.1038/s41467-021-23197-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129080PMC
May 2021

Solvothermal synthesis of porphyrin-ferrocenyl conjugated microporous polymer nanospheres for shape-stable phase change materials with improved latent heat and cyclability.

J Colloid Interface Sci 2021 Aug 24;595:178-186. Epub 2021 Mar 24.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China. Electronic address:

Organic phase change materials (PCMs) have attracted considerable attention for thermal energy storage applications because of their non-toxicity, suitable working temperature range and excellent thermal/chemical stability. However, most traditional organic PCMs have small molecular structures and are prone to leakage during fusion. To address this problem and enhance the shape-stability of organic PCMs, nanosphere-shaped porphyrin-ferrocenyl conjugated microporous polymers (PFCMPs) with high porosity (~ 650 m/g) were solvothermally synthesized using a Diels-Alder reaction between 1,1'-ferrocenedicarboxaldehyde and pyrrole in the presence of glacial acetic acid and anhydrous Lewis acids (FeCl, AlCl, and CuCl). The PFCMPs were then encapsulated with PCMs, that is, 1-octadecanol (ODA), to prepare the composite materials of [email protected] The optimized composite exhibited a high latent heat (up to 153.8 J/g), excellent reversibility (negligible change in latent heat upon 100 cycles of heating-cooling), good shape stability, and long heat storage durability (425 s), making it a promising candidate for solar thermal energy engineering and management.
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http://dx.doi.org/10.1016/j.jcis.2021.03.111DOI Listing
August 2021

Inconsistencies in study eligibility criteria are common between non-Cochrane systematic reviews and their protocols registered in PROSPERO.

Res Synth Methods 2021 May 15;12(3):394-405. Epub 2021 Feb 15.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.

The author should give careful consideration to the study eligibility criteria of systematic reviews (SRs) and follow it after review protocol development to reduce the possibility of manipulation of inclusion. Our aim was to investigate the prevalence of differences in study eligibility criteria between non-Cochrane SRs and their pre-registered protocols on PROSPERO, and determined what changes were involved as well as whether those changes were explained. We searched the protocols registered on PROSPERO platform in the year of 2018 and then selected these protocols which full-text have been published up to June 9, 2020. A random sample (n = 100) was included. Published full-texts were identified through the protocol's final publication citation. The following five key components of study eligibility criteria were compared: participants, intervention(s)/exposure(s), comparator(s), types of study design, and outcome(s). A total of 90% of included SRs exhibited differences in study eligibility criteria, and 59/90 altered in no less than two key components. Only one SR reported and explained the rationale for changes to the individual key component (the definition of exposure). The "Outcome(s)" exhibited the greatest variation, with changes in 61% of the SRs. The "Comparator(s)/control" exhibited the smallest variation, with changes in 20% of the SRs. Differences in study eligibility criteria between the non-Cochrane SRs and their protocols registered on PROSPERO were widespread but were seldom explained. Authors themselves, PROSPERO platform, as well as peer-review journals and their peer-reviewers should play a role in further improving transparency.
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http://dx.doi.org/10.1002/jrsm.1476DOI Listing
May 2021

Deficiency of Mitochondrial Functions and Peroxidation of Frontoparietal Cortex Enhance Isoflurane Sensitivity in Aging Mice.

Front Aging Neurosci 2020 3;12:583542. Epub 2020 Dec 3.

Laboratory of Anesthesia & Critical Care Medicine, Translational Neuroscience Center, National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, China.

: Hypersensitivity to general anesthetics may predict poor postoperative outcomes, especially among the older subjects. Therefore, it is essential to elucidate the mechanism underlying hypersensitivity to volatile anesthetics in the aging population. Given the fact that isoflurane sensitivity increases with aging, we hypothesized that deficiencies of mitochondrial function and elevated oxidative levels in the frontoparietal cortex may contribute to the enhanced sensitivity to isoflurane in aging mice. : Isoflurane sensitivity in aging mice was determined by the concentration of isoflurane that is required for loss of righting reflex (LORR). Mitochondrial bioenergetics of the frontoparietal cortex was measured using a Seahorse XFp analyzer. Protein oxidation and lipid oxidation in the frontoparietal cortex were assessed using the Oxyblot protein oxidation detection kit and thiobarbituric acid reactive substance (TBARS) assay, respectively. Contributions of mitochondrial complex II inhibition by malonate and peroxidation by ozone to isoflurane sensitivity were tested . Besides, effects of antioxidative therapy on mitochondrial function and isoflurane sensitivity in mice were also measured. : The mean concentration of isoflurane that is required for LORR in aging mice (14-16 months old) was 0.83% ± 0.13% (mean ± SD, = 80). Then, the mice were divided into three groups as sensitive group (S group, mean - SD), medium group (M group), and resistant group (R group, mean + SD) based on individual concentrations of isoflurane required for LORR. Activities of mitochondrial complex II and complex IV in mice of the S group were significantly lower than those of the R group, while frontoparietal cortical malondialdehyde (MDA) levels were higher in the mice of S group. Both inhibition of mitochondrial complexes and peroxidation significantly decreased the concentration of isoflurane that is required for LORR . After treatment with idebenone, the levels of lipid oxidation were alleviated and mitochondrial function was restored in aging mice. The concentration of isoflurane that required for LORR was also elevated after idebenone treatment. : Decreased mitochondrial functions and higher oxidative stress levels in the frontoparietal cortex may contribute to the hypersensitivity to isoflurane in aging mice.
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http://dx.doi.org/10.3389/fnagi.2020.583542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744615PMC
December 2020

Upregulation of a homolog by transposon insertion promotes leafy head development in lettuce.

Proc Natl Acad Sci U S A 2020 12 7;117(52):33668-33678. Epub 2020 Dec 7.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture (MOA), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070 Wuhan, People's Republic of China;

Leafy head is a unique type of plant architecture found in some vegetable crops, with leaves bending inward to form a compact head. The genetic and molecular mechanisms underlying leafy head in vegetables remain poorly understood. We genetically fine-mapped and cloned a major quantitative trait locus controlling heading in lettuce. The candidate gene () is a homolog of () from Complementation and CRISPR/Cas9 knockout experiments confirmed the role of in heading. In heading lettuce, there is a CACTA-like transposon inserted into the first exon of (▽). The transposon sequences act as a promoter rather than an enhancer and drive high expression of ▽. The enhanced expression of ▽ is necessary but not sufficient for heading in lettuce. Data from ChIP-sequencing, electrophoretic mobility shift assays, and dual luciferase assays indicate that the LsKN1▽ protein binds the promoter of and down-regulates its expression to alter leaf dorsoventrality. This study provides insight into plant leaf development and will be useful for studies on heading in other vegetable crops.
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http://dx.doi.org/10.1073/pnas.2019698117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776633PMC
December 2020

Quality of interventional animal experiments in Chinese journals: compliance with ARRIVE guidelines.

BMC Vet Res 2020 Nov 26;16(1):460. Epub 2020 Nov 26.

Evidence Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, No.199, Donggang West Road, Lanzhou City, 730000, Gansu Province, China.

Background: In view of the inadequacy and incompleteness of currently-reported animal experiments and their overall poor quality, we retrospectively evaluated the reporting quality of animal experiments published in Chinese journals adhering to the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines.

Results: The databases CNKI, WanFang, VIP, and CBM were searched from inception until July 2018. Two appropriately-trained reviewers screened and extracted articles independently. The ARRIVE guidelines were used to assess the quality of the published reports of animal experiments. The compliance rate of every item was analyzed relative to their date of publication. A total of 4342 studies were included, of which 73.0% had been cited ≤5 times. Only 29.0% (1261/4342) were published in journals listed in the Chinese Science Citation Database. The results indicate that the compliance rate of approximately half of the sub-items (51.3%, 20/39) was less than 50%, of which 65.0% (13/20) was even less than 10%.

Conclusions: The reporting quality of animal experiments in Chinese journals is not at a high level. Following publication of the ARRIVE guidelines in 2010, the compliance rate of the majority of its requirements has improved to some extent. However, less attention has been paid to the ethics and welfare of experimental animals, and a number of specific items in the Methods, Results, and Discussion sections continue to not be reported in sufficient detail. Therefore, it is necessary to popularize the ARRIVE guidelines, advocate researchers to adhere to them in the future, and in particular promote the use of the guidelines in specialized journals in order that the design, implementation, and reporting of animal experiments is promoted, to ultimately improve their quality.
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http://dx.doi.org/10.1186/s12917-020-02664-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690085PMC
November 2020

Frailty as a Predictor of Negative Health Outcomes in Chronic Kidney Disease: A Systematic Review and Meta-Analysis.

J Am Med Dir Assoc 2021 Mar 18;22(3):535-543.e7. Epub 2020 Nov 18.

Evidence-based Nursing Center, School of Nursing, Lanzhou University, Lanzhou, China; Evidence-based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China. Electronic address:

Objectives: To perform a comprehensive evidence synthesis to summarize the impact of frailty on health outcomes in patients with chronic kidney disease (CKD).

Design: Systematic reviews and meta-analysis.

Setting: Electronic searches in PubMed, Embase, Web of Science, CNKI, VIP, CBM, and Wanfang Database were performed. The methodological quality was evaluated using the Newcastle Ottawa Scale (NOS).

Participants: Patients with chronic kidney disease (CKD).

Measurements: Potential clinical outcomes due to frailty.

Results: Eighteen cohort studies incorporating a total of 22,788 participants were included. The overall risk of bias was low. The median reported prevalence of frail and prefrail individuals with CKD was 41.8% (range 2.8-81.5%) and 43.9% (range 19.1-62.7%), respectively. Prefrailty and frailty related to mortality indicated an increased hazard ratio (HR), with a pooled HR of 1.68 [95% confidence interval (CI) 1.46-1.94; P < .001] and 1.48 (95% CI 1.21-1.81; P < .001), respectively. Prefrailty and frailty related to hospitalization with the pooled HR/risk ratio (RR) of 1.56 (95% CI 1.37-1.76; P < .001) and 1.21 (95% CI 0.79-1.85; P = .38), respectively. Similarly, the pooled HR demonstrated a strong correlation between frailty and falls in patients with CKD with HR 1.83 (95% CI 1.40-2.37; P < .001) and no statistical correlation between prefrailty and falls in these patients with pooled HR 1.19 (95% CI 0.44-3.22; P = .73), respectively.

Conclusions And Implications: Frailty is predictive of negative outcomes in patients with CKD, including all-cause mortality, all-cause hospitalization, and falls. Therefore, frailty should be routinely assessed among patients with CKD to prevent poor prognosis, reduce fatality rate, and provide evidence to support future targeted interventions. However, because of the limited amount of information currently in the literature, additional prospective studies are needed to explore the role of prefrailty in predicting adverse outcomes for patients with CKD.
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http://dx.doi.org/10.1016/j.jamda.2020.09.033DOI Listing
March 2021

The Incidence, Risk Factors and In-Hospital Mortality of Acute Kidney Injury in Patients After Surgery for Acute Type A Aortic Dissection: A Single-Center Retrospective Analysis of 335 Patients.

Front Med (Lausanne) 2020 15;7:557044. Epub 2020 Oct 15.

Department of Anesthesiology, West China Hospital, Sichuan University & The Research Units of West China (2018RU012), Chinese Academy of Medical Sciences, Chengdu, China.

Acute kidney injury (AKI) is a common complication of cardiac surgery, which could lead to increased morbidity and mortality. Acute type A aortic dissection (AAAD) is a life-threatening cardiac disease and can be closely related to post-operative AKI. However, data on the incidence of AKI defined by the newest Kidney Disease: Improving Global Outcomes (KDIGO) criteria and in-hospital mortality of a homogeneous population who underwent AAAD are limited. We aimed to investigate the incidence of AKI defined by the KDIGO criteria and the risk factors associated with the outcomes among AAAD-induced AKI patients. We reviewed 335 patients who underwent surgical treatment for AAAD between March 2009 and June 2016. We screened the patients' AKI status and analyzed probably risk factors of AKI and in-hospital mortality. Independent-sample -test or Chi-square test was performed to identify differences between AKI and non-AKI groups and survivors with AKI and non-survivors with AKI, respectively. The logistic regression model was applied to identify independent risk factors. AKI occurred in 71.94% of AAAD patients, including 85 stage 1 (35.26%), 77 stage 2 (31.95%), and 79 stage 3 (32.78%) patients. The in-hospital mortality rate was 21.16%. Logistic regression analysis showed that the body mass index, chronic kidney disease, chronic liver disease, cardiopulmonary bypass duration, red blood cell transfusion, and hypoproteinemia were the independent significant risk factors of the occurrence of post-operative AKI. The risk factors associated with in-hospital mortality among AAAD-induced AKI patients included AKI stage (odds ratio (OR), 3.322), deep hypothermic circulatory arrest (OR, 2.586), lactic acidosis (OR, 3.407), and continuous renal replacement therapy (OR, 3.156). For AAAD patients undergoing surgery, AKI was a common complication, and it increased patients' mortality risk. Therefore, identifying the risk factors of AKI and preventing post-operative AKI are important for improving the post-operative outcomes of AAAD patients. ChiCTR, ChiCTR1900021290. Registered 12 February 2019, http://www.chictr.org.cn/showproj.aspx?proj=35795.
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http://dx.doi.org/10.3389/fmed.2020.557044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593546PMC
October 2020

A phased genome based on single sperm sequencing reveals crossover pattern and complex relatedness in tea plants.

Plant J 2021 01 8;105(1):197-208. Epub 2020 Dec 8.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.

For diploid organisms that are highly heterozygous, a phased haploid genome can greatly aid in functional genomic, population genetic and breeding studies. Based on the genome sequencing of 135 single sperm cells of the elite tea cultivar 'Fudingdabai', we herein phased the genome of Camellia sinensis, one of the most popular beverage crops worldwide. High-resolution genetic and recombination maps of Fudingdabai were constructed, which revealed that crossover (CO) positions were frequently located in the 5' and 3' ends of annotated genes, while CO distributions across the genome were random. The low CO frequency in tea can be explained by strong CO interference, and CO simulation revealed the proportion of interference insensitive CO ranged from 5.2% to 11.7%. We furthermore developed a method to infer the relatedness between tea accessions and detected complex kinship and genetic signatures of 106 tea accessions. Among them, 59 accessions were closely related with Fudingdabai and 31 of them were first-degree relatives. We additionally identified genes displaying allele specific expression patterns between the two haplotypes of Fudingdabai and genes displaying significantly differential expression levels between Fudingdabai and other haplotypes. These results lay the foundation for further investigation of genetic and epigenetic factors underpinning the regulation of gene expression and provide insights into the evolution of tea plants as well as a valuable genetic resource for future breeding efforts.
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http://dx.doi.org/10.1111/tpj.15051DOI Listing
January 2021

High-Yield Synthesis of Pyridyl Conjugated Microporous Polymer Networks with Large Surface Areas: From Molecular Iodine Capture to Metal-Free Heterogeneous Catalysis.

Macromol Rapid Commun 2020 Nov 13;41(22):e2000489. Epub 2020 Oct 13.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China.

Capturing volatile radioactive nuclides including iodine (I or I ) is one of the major problems to be solved for environmental sustainability. Multiple types of functional microporous materials such as metal organic frameworks and covalent organic frameworks have been constructed for iodine emission control. However, most of the microporous materials are limited by their weak binding force with iodine and low stability, leading to low capture efficiencies. Herein, the synthesis of pyridyl conjugated microporous polymer networks with large surface areas (PCMP-Y) up to 1304 m g and high yields up to 95% via a simple Yamamoto cross-coupling reaction, is reported. The PCMP-Y carries amine and pyridine N groups which have stronger interactions with iodine molecules. The high specific surface areas and porosities of PCMP-Y facilitate iodine capture, delivering a maximum adsorption capacity of 4.75 g g in a short time (3 h), which is superior to a majority of porous materials reported. Moreover, the reversible desorption nature of PCMP-Y capturing iodine imparts a platform for metal-free heterogeneous catalyst, which can be applied to synthesize aminobenzothiazole medicines via O -promoted cascade reactions.
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http://dx.doi.org/10.1002/marc.202000489DOI Listing
November 2020

Dissection of the domestication-shaped genetic architecture of lettuce primary metabolism.

Plant J 2020 11 14;104(3):613-630. Epub 2020 Sep 14.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.

Lettuce (Lactuca sativa L.) is an important vegetable crop species worldwide. The primary metabolism of this species is essential for its growth, development and reproduction as well as providing a considerable direct source of energy and nutrition for humans. Here, through investigating 77 primary metabolites in 189 accessions including all major horticultural types and wild lettuce L. serriola we showed that the metabolites in L. serriola were different from those in cultivated lettuce. The findings were consistent with the demographic model of lettuce and supported a single domestication event for this species. Selection signals among these metabolic traits were detected. Specifically, galactinol, malate, quinate and threonate were significantly affected by the domestication process and cultivar differentiation of lettuce. Galactinol and raffinose might have been selected during stem lettuce cultivation as an adaption to the local environments in China. Furthermore, we identified 154 loci significantly associated with the level of 51 primary metabolites. Three genes (LG8749721, LG8763094 and LG5482522) responsible for the levels of galactinol, raffinose, quinate and chlorogenic acid were further dissected, which may have been the target of domestication and/or affected by local adaptation. Additionally, our findings strongly suggest that human selection resulted in reduced quinate and chlorogenic acid levels in cultivated lettuce. Our study thus provides beneficial genetic resources for lettuce quality improvement and sheds light on the domestication and evolution of this important leafy green.
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http://dx.doi.org/10.1111/tpj.14950DOI Listing
November 2020

Bioinformatic identification of prognostic indicators in bladder cancer.

Biomark Med 2020 09 4;14(13):1243-1254. Epub 2020 Aug 4.

Department of Urology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.

Bladder cancer (BC) is one of the most common malignancies with poor prognosis. We aimed to identify a genetic signature for predicting the prognosis of BC. Kaplan-Meier survival and Cox regression analyses were used to construct a prognostic signature using data from The Cancer Genome Atlas.  Fifty four upregulated and 47 downregulated immune-related genes (IRGs) were identified in BC. A prognostic signature based on the expression of five IRGs was determined, which was moderately accurate in the prognosis of tumors. The prognostic signature was correlated with tumor stage, tumor burden and lymph node metastasis. The expression of IRGs were associated with immune infiltration. We determined a five gene signature, which correlates with the prognosis of BC patients, providing additional information for effective treatment.
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http://dx.doi.org/10.2217/bmm-2020-0316DOI Listing
September 2020

The role of BAMBI in regulating adipogenesis and myogenesis and the association between its polymorphisms and growth traits in cattle.

Mol Biol Rep 2020 Aug 1;47(8):5963-5974. Epub 2020 Aug 1.

College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, Shaanxi, China.

Bone morphogenic protein and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein that affects the growth, development and muscle regeneration of the body by regulating the TGF-β, BMP and Wnt signaling pathways. Studies have found that BAMBI has important regulatory functions in skeletal muscle and preadipocytes in vivo and in vitro. However, research on this protein in cattle is lacking. In this study, to determine the role of BAMBI in the growth and development of cattle, we first found that the expression of BAMBI in adipose tissue and longissimus muscle of newborn and adult Qinchuan beef cattle was significantly different. Then we showed that BAMBI knockdown promoted the differentiation of bovine preadipocytes and suppressed myoblast myogenesis, as indicated by the increased lipid droplets and the decreased myotubes, as well as the corresponding significant changes in the expression of PPARγ, C/EBPα, C/EBPβ, FABP4, MyoD, MyoG and Myf6. Finally, to further verify the effect of BAMBI on the growth performance of cattle, we identified seven novel SNPs in the BAMBI genomic region, which were significantly correlated with one or more growth traits (p < 0.05). Furthermore, individuals with haplotype H1H4 (TC-GA-CT-CA-AT-AT-AG) had a higher body and carcass quality than those with other haplotypes (p < 0.05). In brief, BAMBI may be a functional gene for the differentiation of bovine preadipocytes and myoblasts, and variations in the BAMBI genomic region, especially the combined haplotype H1H4, may benefit marker-assisted selection in cattle.
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http://dx.doi.org/10.1007/s11033-020-05670-6DOI Listing
August 2020

Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1.

Life Sci 2020 Oct 26;258:118151. Epub 2020 Jul 26.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300353, China. Electronic address:

Aims: Hepatic glucose metabolism involves a variety of catabolic and anabolic pathways, and the dynamic balance of glucose metabolism is regulated in response to environmental and nutritional changes. The molecular mechanism of glucose metabolism in liver is complex and has not been fully elucidated so far. In this study, we hope to elucidate the target and mechanism of cinnamaldehyde (CA) in regulating glucose metabolism.

Materials And Methods: Molecular image tracing and magnetic capture in combination with an alkynyl-CA probe (Al-CA) was used to show CA covalently binds to α-enolase (ENO1) in both mouse liver and HepG2 cells. Accurate metabolic flow assays subsequently demonstrated that the utilization of glycogenic amino acids and the biosynthesis of tricarboxylic acid (TCA) cycle intermediates were strengthened, which was detected using nontargeted and targeted metabolomics analyses.

Key Findings: Our study shows that CA covalently bonds with ENO1, which affects the stability and activity of ENO1 and changes the dynamic balance of glucose metabolism. The interruption of gluconeogenic reflux by ENO1 enhanced TCA cycle, and eventually led to a decrease in blood glucose and the improvement of mitochondrial efficiency.

Significance: These results provide a detailed description of how CA maintains the dynamic balance of glucose utilization and improves energy metabolism.
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http://dx.doi.org/10.1016/j.lfs.2020.118151DOI Listing
October 2020

Genome assembly of wild tea tree DASZ reveals pedigree and selection history of tea varieties.

Nat Commun 2020 07 24;11(1):3719. Epub 2020 Jul 24.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.

Wild teas are valuable genetic resources for studying domestication and breeding. Here we report the assembly of a high-quality chromosome-scale reference genome for an ancient tea tree. The further RNA sequencing of 217 diverse tea accessions clarifies the pedigree of tea cultivars and reveals key contributors in the breeding of Chinese tea. Candidate genes associated with flavonoid biosynthesis are identified by genome-wide association study. Specifically, diverse allelic function of CsANR, CsF3'5'H and CsMYB5 is verified by transient overexpression and enzymatic assays, providing comprehensive insights into the biosynthesis of catechins, the most important bioactive compounds in tea plants. The inconspicuous differentiation between ancient trees and cultivars at both genetic and metabolic levels implies that tea may not have undergone long-term artificial directional selection in terms of flavor-related metabolites. These genomic resources provide evolutionary insight into tea plants and lay the foundation for better understanding the biosynthesis of beneficial natural compounds.
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http://dx.doi.org/10.1038/s41467-020-17498-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381669PMC
July 2020

IL-17a exacerbates hepatic ischemia-reperfusion injury in fatty liver by promoting neutrophil infiltration and mitochondria-driven apoptosis.

J Leukoc Biol 2020 11 12;108(5):1603-1613. Epub 2020 Jun 12.

Department of Surgery, HKU-SZH & Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Hepatic ischemia-reperfusion (IR) injury is a critical issue during liver transplantation (LT). Recent studies have demonstrated that IL-17a contributes to IR injury and steatohepatitis. However, the underlying mechanism is not understood. This study aimed to examine the role of IL-17a on hepatic IR injury in fatty liver and to investigate the underlying mechanisms. The correlation between serum IL-17a levels and liver function was analyzed in LT patients receiving fatty (n = 42) and normal grafts (n = 44). Rat LT model was applied to validate the clinical findings. IL-17a knockout (KO) and wild-type mice were fed with high-fat diets to induce fatty liver and subjected to hepatic IR injury with major hepatectomy. Frequency of circulating neutrophils and IL-17a expression on PBMCs were analyzed by flow cytometry. Mitochondrial outer membrane permeabilization (MOMP) was examined by a living intravital image system. Serum IL-17a was elevated after human LT, especially with fatty grafts. The aspartate aminotransferase and alanine transaminase levels were increased in recipients with fatty grafts compared with normal grafts. In rat LT model, the intragraft IL-17a expression was significantly higher in fatty grafts than normal ones post-LT. KO of IL-17a in mice notably attenuated liver damage after IR injury in fatty liver, characterized by better-preserved liver architecture, improved liver function, and reduced neutrophil infiltration. MOMP triggered cell death after hepatic IR injury in a caspase-independent way via IL-17a/NF-κB signaling pathway. KO of IL-17a protected the fatty liver against IR injury through the suppression of neutrophil infiltration and mitochondria-driven apoptosis.
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http://dx.doi.org/10.1002/JLB.3MA0520-716RDOI Listing
November 2020

ADTRP regulates TFPI expression via transcription factor POU1F1 involved in coronary artery disease.

Gene 2020 Aug 20;753:144805. Epub 2020 May 20.

Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan 430074, PR China. Electronic address:

Genomic variants in both ADTRP and TFPI genes are associated with risk of coronary artery disease (CAD). ADTRP regulates TFPI expression and endothelial cell functions involved in the initiation of atherosclerotic CAD. ADTRP also specifies primitive myelopoiesis and definitive hematopoiesis by upregulating TFPI expression. However, the underlying molecular mechanism is unknown. Here we show that transcription factor POU1F1 is the key by which ADTRP regulates TFPI expression. Luciferase reporter assays, chromatin-immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) in combination with analysis of large and small deletions of the TFPI promoter/regulatory region were used to identify the molecular mechanism by which ADTRP regulates TFPI expression. Genetic association was assessed using case-control association analysis and phenome-wide association analysis (PhenGWA). ADTRP regulates TFPI expression at the transcription level in a dose-dependent manner. The ADTRP-response element was localized to a 50 bp region between -806 bp and -756 bp upstream of TFPI transcription start site, which contains a binding site for POU1F1. Deletion of POU1F1-binding site or knockdown of POU1F1 expression abolished ADTRP-mediated transcription of TFPI. ChIP and EMSA demonstrated that POU1F1 binds to the ADTRP response element. Genetic analysis identified significant association between POU1F1 variants and risk of CAD. PhenGWA identified other phenotypic traits associated with the ADTRP-POU1F1-TFPI axis such as lymphocyte count (ADTRP), waist circumference (TFPI), and standing height (POU1F1). These data identify POU1F1 as a transcription factor that regulates TFPI transcription in response to ADTRP, and link POU1F1 variants to risk of CAD for the first time.
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http://dx.doi.org/10.1016/j.gene.2020.144805DOI Listing
August 2020

The effectiveness of acupuncture for Parkinson's disease: An overview of systematic reviews.

Complement Ther Med 2020 May 12;50:102383. Epub 2020 Mar 12.

School of Public Health, Lanzhou University, Lanzhou 730000, China; Evidence Based Social Science Research Center, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, Lanzhou 730000, China; Evidence Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. Electronic address:

Objectives: Acupuncture is an alternative therapy for Parkinson's disease (PD), but its efficacy and safety are controversial. This overview aimed to summarize the existing evidence from systematic reviews (SRs) and meta-analyses (MAs) in order to assess the effectiveness of acupuncture as a treatment for PD.

Methods: Seven electronic databases were searched from their inception until July 2019. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) and Assessment of Multiple Systematic Reviews 2 (AMSTAR2) checklists were used to assess evidence quality and methodological quality, respectively. The outcomes of study were calculated using mean differences (MDs) and risk ratios (RRs) with 95 % confidence intervals (CIs). A meta-analysis was performed using RevMan 5.3 software.

Results: A total of 12 SRs/MAs were included. All 12 SRs/MAs had more than one critical weakness in AMSTAR 2 and were considered of critically low methodological quality. The quality of evidence was unsatisfactory according to the GRADE checklist. Meta-analyses showed that acupuncture combined with drug for the treatment of PD can significantly improve the total effectiveness rate compared with drug alone (RR = 1.25, 95 % CI 1.16-1.34, P < 0.001). It was also found that acupuncture combined with drug significantly improved the UPDRS I-IV total summed scores (WMD=-6.18, 95 % CI -10.32 to -2.04, P < 0.001) and Webster scores (WMD=-4.20, 95 % CI -7.59 to -0.81, P < 0.001).

Conclusion: Acupuncture might improve the UPDRS score, Webster score, and total effective rate in treatment of PD. It might be a safe and useful adjunctive treatment for patients with PD. However, we should interpret the findings of these reviews with caution, considering the overall limited methodological and reporting quality.
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http://dx.doi.org/10.1016/j.ctim.2020.102383DOI Listing
May 2020

Characterizing pancreatic β-cell heterogeneity in the streptozotocin model by single-cell transcriptomic analysis.

Mol Metab 2020 07 2;37:100982. Epub 2020 Apr 2.

Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, China. Electronic address:

Objectives: The streptozotocin (STZ) model is widely used in diabetes research. However, the cellular and molecular states of pancreatic endocrine cells in this model remain unclear. This study explored the molecular characteristics of islet cells treated with STZ and re-evaluated β-cell dysfunction and regeneration in the STZ model.

Methods: We performed single-cell RNA sequencing of pancreatic endocrine cells from STZ-treated mice. High-quality sequencing data from 2,999 cells were used to identify clusters via Louvain clustering analysis. Principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE), uniform manifold approximation and projection (UMAP), force-directed layout (FDL), and differential expression analysis were used to define the heterogeneity and transcriptomic changes in islet cells. In addition, qPCR and immunofluorescence staining were used to confirm findings from the sequencing data.

Results: Untreated β-cells were divided into two populations at the transcriptomic level, a large high-Glut2 expression (Glut2) population and a small low-Glut2 expression (Glut2) population. At the transcriptomic level, Glut2 β-cells in adult mice did not represent a developmentally immature state, although a fraction of genes associated with β-cell maturation and function were downregulated in Glut2 cells. After a single high-dose STZ treatment, most Glut2 cells were killed, but Glut2 cells survived and over time changed to a distinct cell state. We did not observe conversion of Glut2 to Glut2 β-cells up to 9 months after STZ treatment. In addition, we did not detect transcriptomic changes in the non-β endocrine cells or a direct trans-differentiation pathway from the α-cell lineage to the β-cell lineage in the STZ model.

Conclusions: We identified the heterogeneity of β-cells in both physiological and pathological conditions. However, we did not observe conversion of Glut2 to Glut2 β-cells, transcriptomic changes in the non-β endocrine cells, or direct trans-differentiation from the α-cell lineage to the β-cell lineage in the STZ model. Our results clearly define the states of islet cells treated with STZ and allow us to re-evaluate the STZ model widely used in diabetes studies.
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http://dx.doi.org/10.1016/j.molmet.2020.100982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184252PMC
July 2020

β-Cyclodextrin-derived Room Temperature Macromolecular Ionic Liquids by PEGylated Anions.

Macromol Rapid Commun 2020 Apr 13;41(8):e1900576. Epub 2020 Mar 13.

Stockholm University, Svante Arrheniusvag 16C, 10691, Stockholm, Sweden.

A series of cyclodextrin-derived room temperature macromolecular ionic liquids carrying rather low glass transition temperatures of -20 to -40 °C are synthesized via sequential esterification, quaternization, and anion metathesis reactions. In addition to being ionic in nature, they are viscous liquids at room temperature with more fluidic behavior at elevated temperatures. They serve as a solvent for organic dyes or iodine separation via a liquid-liquid extraction approach. This strategy is useful for the development of various sugar (macro)molecule-based functional ionic liquids as well as macromolecular ionic liquids.
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http://dx.doi.org/10.1002/marc.201900576DOI Listing
April 2020

Enhancing crystal growth using polyelectrolyte solutions and shear flow.

Nature 2020 03 4;579(7797):73-79. Epub 2020 Mar 4.

Center for Soft and Living Matter, Institute for Basic Science, Ulsan, South Korea.

The ability to grow properly sized and good quality crystals is one of the cornerstones of single-crystal diffraction, is advantageous in many industrial-scale chemical processes, and is important for obtaining institutional approvals of new drugs for which high-quality crystallographic data are required. Typically, single crystals suitable for such processes and analyses are grown for hours to days during which any mechanical disturbances-believed to be detrimental to the process-are carefully avoided. In particular, stirring and shear flows are known to cause secondary nucleation, which decreases the final size of the crystals (though shear can also increase their quantity). Here we demonstrate that in the presence of polymers (preferably, polyionic liquids), crystals of various types grow in common solvents, at constant temperature, much bigger and much faster when stirred, rather than kept still. This conclusion is based on the study of approximately 20 diverse organic molecules, inorganic salts, metal-organic complexes, and even some proteins. On typical timescales of a few to tens of minutes, these molecules grow into regularly faceted crystals that are always larger (with longest linear dimension about 16 times larger) than those obtained in control experiments of the same duration but without stirring or without polymers. We attribute this enhancement to two synergistic effects. First, under shear, the polymers and their aggregates disentangle, compete for solvent molecules and thus effectively 'salt out' (that is, induce precipitation by decreasing solubility of) the crystallizing species. Second, the local shear rate is dependent on particle size, ultimately promoting the growth of larger crystals (but not via surface-energy effects as in classical Ostwald ripening). This closed-system, constant-temperature crystallization driven by shear could be a valuable addition to the repertoire of crystal growth techniques, enabling accelerated growth of crystals required by the materials and pharmaceutical industries.
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http://dx.doi.org/10.1038/s41586-020-2042-1DOI Listing
March 2020

The effectiveness of music therapy for patients with cancer: A systematic review and meta-analysis.

J Adv Nurs 2020 May 19;76(5):1111-1123. Epub 2020 Feb 19.

Evidence Based Social Science Research Center, School of Public Health, Lanzhou University, Lanzhou, China.

Aims: To assess the effectiveness of music therapy on the quality of life, anxiety, depression and pain of patients with cancer.

Design: Systematic review.

Data Sources: Five electronic databases were searched in September 2018 for randomized controlled trials evaluating music therapy for patients with cancer.

Review Methods: The quality of the studies was assessed using the risk of bias tool recommended by the Cochrane Handbook Version 5.1.0 and the Revman version 5.3 software was used to perform the meta-analysis. The outcomes were overall quality of life, anxiety, depression and pain.

Results: A total of 19 trials evaluating 1,548 patients were included in this study, of which 765 were in the control group and 783 in the experimental group. Compared with standard care, music therapy can significantly increase the score of the overall quality of life in patients with cancer. In addition, music therapy was found to be more effective for decreasing the score of anxiety, depression and pain.

Conclusion: Music therapy can improve the overall quality of life of patients with cancer, with an observed optimal intervention duration of 1-2 months. Meanwhile, anxiety, depression and pain are improved as well. Nevertheless, high-quality trials are still needed to further determine the effects of music intervention in supportive cancer care.
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http://dx.doi.org/10.1111/jan.14313DOI Listing
May 2020

Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine.

Cancers (Basel) 2020 Jan 29;12(2). Epub 2020 Jan 29.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300353, China.

At present, melanoma is a common malignant tumor with the highest mortality rate of all types of skin cancer. Although the first option for treating melanoma is with chemicals, the effects are unsatisfactory and include poor medication response and high resistance. Therefore, developing new medicines or a novel combination approach would be a significant breakthrough. Here, we present cinnamaldehyde (CA) as a potential candidate, which exerted an antitumor effect in melanoma cell lines. Chemical biology methods of target fishing, molecular imaging, and live cell tracing by an alkynyl-CA probe revealed that the α-enolase (ENO1) protein was the target of CA. The covalent binding of CA with ENO1 changed the stability of the ENO1 protein and affected the glycolytic activity. Furthermore, our results demonstrated that dacarbazine (DTIC) showed a high promoting effect with CA for antimelanoma both in vivo and in vitro. The combination improved the DTIC cell cycle arrest in the S phase and markedly impacted melanoma growth. As a covalent inhibitor of ENO1, CA combined with DTIC may be beneficial in patients with drug resistance in antimelanoma therapy.
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http://dx.doi.org/10.3390/cancers12020311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072165PMC
January 2020

Yellow Emission Obtained by Combination of Broadband Emission and Multi-Peak Emission in Garnet Structure NaYMgVO: Dy Phosphor.

Molecules 2020 Jan 27;25(3). Epub 2020 Jan 27.

Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences, Beijing 100083, China.

The fabrication and luminescent performance of novel phosphors NaYMgVO:Dy were investigated by a conventional solid-state reaction method. Under near-UV light, the NaYMgVO host self-activated and released a broad emission band (400-700 nm, with a peak at 524 nm) ascribable to charge transfer in the (VO) groups. Meanwhile, the NaYMgVO:Dy phosphors emitted bright yellow light within both the broad emission band of the (VO) groups and the sharp peaks of the Dy ions at 490, 582, and 663 nm at a quenching concentration of 0.03 mol. The emission of the as-prepared NaYMgVO:Dy phosphors remained stable at high temperatures. The obtained phosphors, commercial YO:Eu red phosphors, and BaMgAlO:Eu blue phosphors were packed into a white light-emitting diode (WLED) device with a near-UV chip. The designed WLED emitted bright white light with good chromaticity coordinates (0.331, 0.361), satisfactory color rendering index (80.2), and proper correlation to a color temperature (7364 K). These results indicate the potential utility of NaYMgVO:Dy phosphor as a yellow-emitting phosphor in solid-state illumination.
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http://dx.doi.org/10.3390/molecules25030542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038123PMC
January 2020

Effects of Salt Stimulation on Lunasin Accumulation and Activity during Soybean Germination.

Foods 2020 Jan 22;9(2). Epub 2020 Jan 22.

College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250353, China.

Lunasin, a bioactive peptide, was originally found in soybeans, and it has exhibited multiple biological functions. On the basis of previous studies, salt stress was found able to induce changes in many polypeptides and translatable mRNA levels in plants. Salt stress was applied to soybean germination, with water treatment as a control group, to evaluate the effects of salt stimulation on lunasin accumulation and activity during soybean germination. Lunasin content gradually increased in the control group during germination, reached the highest level after six hours of imbibition, and then slowly decreased. Under salt exposure, lunasin content showed a similar trend to that of the control group. The lunasin content in salt-treated soybean was significantly higher than that in the control group. Lunasin peptide was purified from soybean after six hours of imbibition and it was then used for function evaluation. Purified lunasin from salt-stress-germinated soybean (6 h-LSGS) exhibited stronger antioxidant activity than lunasin from water-treatment-germinated soybean (6 h-LWGS) and soybean seed without imbibition (DRY). The 6 h-LSGS presented anti-inflammatory activity on LPS-induced macrophage cells ( < 0.05) by suppressing the release of nitric oxide (NO) and proinflammatory cytokines, including IL-1 and IL-6. The gene expression of , IL-1, IL-6, and TNF-α was significantly inhibited by 6 h-LSGS. Further, 6 h-LSGS exhibited superior antiproliferation activity on human breast-cancer cells MDA-MB-231 when compared to 6 h-LWGS and DRY. Overall, this study offers a feasible elicitation strategy for enhancing lunasin accumulation and its properties in soybean for possible use in functional food.
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http://dx.doi.org/10.3390/foods9020118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073574PMC
January 2020

Bioinformatic identification of renal cell carcinoma microenvironment-associated biomarkers with therapeutic and prognostic value.

Life Sci 2020 Feb 8;243:117273. Epub 2020 Jan 8.

Department of Urology, Gaozhou People's Hospital, Maoming 525200, China. Electronic address:

Renal cell carcinoma (RCC) is the ninth most prevalent form of malignancy worldwide. The tumor microenvironment significantly affects gene expression in tumor tissues, which subsequently impacts the prognosis of RCC patients. Available datasets such as The Cancer Genome Atlas (TCGA) can be utilized to improve diagnostic methods and search for novel tumor therapeutic targets and prognostic biomarkers. The current study used the ESTIMATE algorithm to explore the immune and stromal components in RCC. Differentially expressed genes (DEGs) were identified by comparing the gene expression patterns in groups with high and low immune/stromal scores. Functional enrichment analysis was conducted and Kaplan-Meier survival curves were plotted to explore the functions of the DEGs in the tumorigenesis, progression, and prognosis of RCC. Our results revealed that immune and stromal scores are associated with specific clinicopathologic variables in RCC. These variables include gender, tumor grade, tumor stage, tumor size, distant metastasis and prognosis. A total of 48 upregulated and 47 downregulated genes were obtained. Functional enrichment analysis demonstrated a correlation between DEGs and the tumor microenvironment, tumor immune response and RCC tumorigenesis. Kaplan-Meier survival curves showed that 43 out of the 48 identified tumor microenvironment related genes are involved in the prognosis of RCC. Three genes, IL10, IGLL5 and POU2AF1, were selected as the hub genes, and their kinase targets were identified as MAPK1 and PPKCA. A positive correlation was obtained between the expression of IL/POU2AF1 and the abundance of six immune cells. Our study provides potential biomarkers for the therapy and prognosis of RCC.
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http://dx.doi.org/10.1016/j.lfs.2020.117273DOI Listing
February 2020

TP53INP2 Promotes Bovine Adipocytes Differentiation Through Autophagy Activation.

Animals (Basel) 2019 Dec 2;9(12). Epub 2019 Dec 2.

College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.

Tumor protein p53 inducible nuclear protein 2 (TP53INP2) is a key positive regulator of autophagy, and it has been shown to modulate adipocyte differentiation. However, the molecular mechanism involved in autophagy regulation during adipocyte differentiation has not been clarified. Our experiments were intended to investigate whether TP53INP2 is involved in the regulation of autophagy during bovine adipocyte differentiation and how TP53INP2 affects the differentiation of bovine adipocytes. In our research, using RT-qPCR and Western blot methods, we found that the overexpression of TP53INP2 resulted in the upregulation of adipogenesis and autophagy-related genes, and autophagy flux and the degree of differentiation were detected by LipidTOX™ Deep Red Neutral Lipid staining and dansylcadaverine staining, respectively. The knockdown of TP53INP2 produced results that were the inverse of those produced by the overexpression of TP53INP2. Overall, our results suggested that TP53INP2 can activate autophagy during the early stage of differentiation in bovine adipocytes and positively regulate adipocyte differentiation by affecting autophagy. Additionally, peroxisome proliferator-activated receptor gamma (PPARγ) also contributed to the function of TP53INP2 in modulating adipocyte differentiation.
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http://dx.doi.org/10.3390/ani9121060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940805PMC
December 2019

Cancer Targeted Gene Therapy for Inhibition of Melanoma Lung Metastasis with eIF3i shRNA Loaded Liposomes.

Mol Pharm 2020 01 10;17(1):229-238. Epub 2019 Dec 10.

State Key Laboratory of Biotherapy/Geriatrics and Cancer Center, West China Hospital and Collaborative Innovation Center for Biotherapy , Sichuan University , Chengdu , 610041 , China.

Eukaryotic translation initiation factors 3i (eIF3i) is a proto-oncogene that is overexpressed in various tumors, reducing its expression by eIF3i shRNA is a promising strategy to inhibit tumor growth or metastasis. Tumor cell is the target of eIF3i shRNA so that tumor-site accumulation could be important for fulfilling its therapeutic effect. Thus, the iRGD modified liposome (R-LP) was rationally synthesized to enhance the antitumor effect by active targeted delivery of eIF3i shRNA to B16F10 melanoma cells. R-LP encapsulating eIF3i shRNA gene (R-LP/sheIF3i) were prepared by a film dispersion method. The transfection experiment proves that R-LP could effectively transfect B16F10 cells. R-LP/sheIF3i notably restrained the migration, invasion, and adhesion of melanoma cells . In a mouse model of lung metastasis, R-LP/sheIF3i administered by intravenous injection suppressed pulmonary metastasis of melanoma by dramatically downregulated eIF3i expression and subsequently inhibiting tumor neovascularization and tumor cells proliferation . Our results provide a basis for tumor cells targeting strategies to reduce the expression of eIF3i by RNAi in the treatment of tumor metastasis.
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http://dx.doi.org/10.1021/acs.molpharmaceut.9b00943DOI Listing
January 2020

Poly(Ionic Liquid)-Derived Graphitic Nanoporous Carbon Membrane Enables Superior Supercapacitive Energy Storage.

ACS Nano 2019 Sep 13;13(9):10261-10271. Epub 2019 Sep 13.

Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering , Huazhong University of Science and Technology , Wuhan , 430074 , China.

High energy/power density, capacitance, and long-life cycles are urgently demanded for energy storage electrodes. Porous carbons as benchmark commercial electrode materials are underscored by their (electro)chemical stability and wide accessibility, yet are often constrained by moderate performances associated with their powdery status. Here controlled vacuum pyrolysis of a poly(ionic liquid) membrane template, advantageous features including good conductivity (132 S cm at 298 K), interconnected hierarchical pores, large specific surface area (1501 m g), and heteroatom doping are realized in a single carbon membrane electrode. The structure synergy at multiple length scales enables large areal capacitances both for a basic aqueous electrolyte (3.1 F cm) and for a symmetric all-solid-state supercapacitor (1.0 F cm), together with superior energy densities (1.72 and 0.14 mW h cm, respectively) without employing a current collector. In addition, theoretical calculations verify a synergistic heteroatom co-doping effect beneficial to the supercapacitive performance. This membrane electrode is scalable and compatible for device fabrication, highlighting the great promise of a poly(ionic liquid) for designing graphitic nanoporous carbon membranes in advanced energy storage.
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http://dx.doi.org/10.1021/acsnano.9b03514DOI Listing
September 2019