Publications by authors named "Weiwei Wang"

1,101 Publications

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Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing From Different Bovine Faeces in China.

Front Vet Sci 2022 1;9:738904. Epub 2022 Aug 1.

Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science (CAAS), Lanzhou, China.

CTX-M extended spectrum beta-lactamase-producing cause severe health hazards in livestock breeding. To date, little is known about antibiotic resistance differences among bacterial isolates from yaks, cows, and beef cattle; therefore, the aims of this study were to analyse the prevalence of CTX-M-producing in yak, beef cattle, and dairy cattle feces from different provinces in China. A total of 790 fecal samples from yaks, beef cattle, and dairy cows were used. Among all the samples, 523 non duplicate isolates were identified, and 29.6% of samples harbored CTX-M producers. The results showed that these strains harbored 15 clusters of CTX-M genes: CTX-M-79, CTX-M-55, CTX-M-15, CTX-M-14, CTX-M-28, CTX-M-179, CTX-M-65, CTX-M-24, CTX-M-27, CTX-M-102, CTX-M-105, CTX-M-173, CTX-M-238, CTX-M-196, and CTX-M-10. The dominant resistance genes were CTX-M-15, CTX-M-14, and CTX-M-55. Moreover, the distribution of CTX-M genes was related to geographical region. Based on the above findings, we reasoned that bovines are potential reservoirs of antibiotic resistance, and this problem should be given adequate attention.
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http://dx.doi.org/10.3389/fvets.2022.738904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376260PMC
August 2022

Toxicity mechanisms regulating bone differentiation and development defects following abnormal expressions of miR-30c targeted by triclosan in zebrafish.

Sci Total Environ 2022 Aug 13;850:158040. Epub 2022 Aug 13.

Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China; School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China. Electronic address:

As a ubiquitous environmental estrogen-disrupting chemical, triclosan (TCS) can induce severe osteotoxicity; however, the underlying molecular mechanisms remain uncertain. Herein, we evaluated the toxic effects of TCS on the development of cartilage and osteogenesis in 5-dpf zebrafish. Under TCS exposure from 62.5 to 250 μg/L, several osteodevelopmental malformations were observed, such as defect of craniofacial cartilage, pharyngeal arch cartilage dysplasia, and impairments on skeletal mineralization. Further, the morphology of mature chondrocytes became swollen and deformed, their number decreased, nucleus displacement occurred, and most immature chondrocytes were crowded at both ends of ceratobranchial. SEM observation of larval caudal fin revealed that, the layer of collagen fibers and the mineralized calcium nodules were significantly decreased, with the collagen fibers becoming shorter upon TCS exposure. The activity of bone-derived alkaline phosphatase significantly reduced, and marker functional genes related to cartilage and osteoblast development were abnormally expressed. RNA-seq and bioinformatics analysis indicated, that changes in marker genes intimately related to the negative regulation of miR-30c-5p overexpression targeted by TCS, and the up-regulation of miR-30c induced bone developmental defects by inhibiting the bone morphogenetic protein (BMP) signaling pathway. These findings were confirmed by artificially intervening the expression of miR-30c and using BMP pathway agonists in vivo. In sum, TCS induced osteototoxicity by targeting miR-30c up-regulation and interfering in the BMP signaling pathway. These findings enhance mechanistic understanding of TCS-induced spontaneous bone disorders and bone metastatic diseases. Further research is necessary to monitor chronic TCS-exposure levels in surrounding environments and develop relevant safety precautions based on TCS environmental risk.
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http://dx.doi.org/10.1016/j.scitotenv.2022.158040DOI Listing
August 2022

A novel Diaphorobacter sp. strain isolated from saponification wastewater shows highly efficient phenanthrene degradation.

Environ Res 2022 Aug 11;214(Pt 3):114047. Epub 2022 Aug 11.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, And School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, are a type of organic pollutants that exist widely in the environment. Of the currently known degradation methods, bioremediation is a desirable and feasible option. A novel Diaphorobacter sp. Strain MNS-0 was isolated from saponification wastewater and showed the ability to degrade phenanthrene, fluorene, acenaphthene, anthracene, benzo[a]anthracene, or chrysene using phenanthrene as the sole carbon source. Gas chromatography mass spectroscopy analysis of catabolic intermediates indicates that phenanthrene degradation occurs through the phthalic acid pathway in strain MNS-0. Genome sequencing shows that strain MNS-0 has two plasmids and one chromosome containing a presumptive phenanthrene degradation gene cluster. Strain MNS-0 was able to completely degrade 100 mg/L phenanthrene within 40 h and tolerate up to 10 g/L NaCl at pH 9.0, while maintaining phenanthrene degradation activity. We thus propose that strain MNS-0 is an effective degrader for bioremediation of PAHs pollution, even in relatively harsh alkali environments such as saponification wastewater.
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http://dx.doi.org/10.1016/j.envres.2022.114047DOI Listing
August 2022

Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against .

Int J Mol Sci 2022 Aug 8;23(15). Epub 2022 Aug 8.

Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou 730050, China.

As an opportunistic pathogen, ( forms biofilm that increases the virulence of bacteria and antibiotic resistance, posing a serious threat to human and animal health. Recently, ginkgetin (Gin) has been discovered to have antiinflammatory, antioxidant, and antitumor properties. In the present study, we evaluated the antibiofilm and antibacterial synergist of Gin against . Additionally, Alamar Blue assay combined with confocal laser scanning microscope (CLSM) and crystal violet (CV) staining was used to evaluate the effect of antibiofilm and antibacterial synergist against . Results showed that Gin reduces biofilm formation, exopolysaccharide () production, and motility against without limiting its growth and metabolic activity. Furthermore, we identified the inhibitory effect of Gin on AI-2 signaling molecule production, which showed apparent anti-quorum sensing (QS) properties. The qRT-PCR also indicated that Gin reduced the transcription of curli-related genes (, ), flagella-formation genes (, , , ), and QS-related genes (, , , ). Moreover, Gin showed obvious antibacterial synergism to overcome antibiotic resistance in with marketed antibiotics, including gentamicin, colistin B, and colistin E. These results suggested the potent antibiofilm and novel antibacterial synergist effect of Gin for treating infections.
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http://dx.doi.org/10.3390/ijms23158809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369100PMC
August 2022

The diagnostic utility of IL-10, IL-17, and PCT in patients with sepsis infection.

Front Public Health 2022 22;10:923457. Epub 2022 Jul 22.

Department of Laboratory Medicine, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Objective: The purpose of this study is to determine the diagnostic value and net clinical benefit of interleukin-10 (IL-10), interleukin-17 (IL-17), procalcitonin (PCT), and combination tests in patients with sepsis, which will serve as a standard for sepsis early detection.

Patients And Methods: An investigation of 84 sepsis patients and 81 patients with local inflammatory diseases admitted to the ICU of Tongji University Hospital in 2021. In addition to comparing inter-group variability, indicators relevant to sepsis diagnosis and therapy were screened.

Results: LASSO regression was used to examine PCT, WBC, CRP, IL-10, IFN-, IL-12, and IL-17. Multivariate logistic regression linked IL-10, IL-17, and PCT to sepsis risk. The AUC values of IL-10, IL-17, PCT, and the combination of the three tests were much higher than those of standard laboratory infection indicators. The combined AUC was greater than the sum of IL-10, IL-17, and PCT ( < 0.05). A clinical decision curve analysis of IL-10, IL-17, PCT, and the three combined tests found that the three combined tests outperformed the individual tests in terms of total clinical benefit rate. To predict the risk of sepsis using IL-10, IL-17, and PCT had an AUC of 0.951, and the model's predicted probability was well matched. An examination of the nomogram model's clinical value demonstrated a considerable net therapeutic benefit between 3 and 87%.

Conclusion: The IL-10, IL-17, and PCT tests all have a high diagnostic value for patients with sepsis, and the combination of the three tests outperforms the individual tests in terms of diagnostic performance, while the combined tests have a higher overall clinical benefit rate.
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http://dx.doi.org/10.3389/fpubh.2022.923457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355284PMC
August 2022

Electrocardiogram Parameters That Affect the Success Rate of Radiofrequency Ablation in Patients with Outflow Tract Ventricular Premature Complexes.

Cardiovasc Ther 2022 22;2022:8160144. Epub 2022 Jul 22.

Department of Cardiology, Fujian Medical University Union Hospital, Fuzhou 350001, China.

Objectives: The objectives of this study are to assess the efficacy of radiofrequency catheter ablation (RFCA) in patients with outflow tract (OT) ventricular premature complexes (VPCs) and to explore the electrocardiographic (ECG) features of initially successful procedures.

Methods: Based on the outcome of ablation, 154 consecutive patients with OT-VPCs who underwent RFCA from January 2017 to December 2019 were divided into two groups. The rate of successful procedures and the ECG features were analyzed and compared between the two groups.

Results: The highest success rate was found in patients with VPCs from the right ventricular outflow tract (RVOT), and the lowest success rate was evident among patients with complexes from both the RVOT and the left ventricular OT (LVOT). The patients with successful procedures (136) reflected a lower pseudo delta wave ratio (16.2% vs. 44.4%, < 0.01), a smaller R-wave amplitude in lead V1 (V1) (0.23 ± 0.24 mV vs. 0.35 ± 0.44 mV, < 0.05), shorter intrinsicoid deflection time in lead V2 (V2) (44.00 ± 18.33 ms vs. 57.41 ± 20.67 ms, < 0.01), a shorter RS duration in V2 (93.67 ± 21.33 ms vs. 106.93 ± 18.76 ms, P < 0.01), and smaller R/S-waveratios in V2. Furthermore, multivariate analysis demonstrated that RS duration in V2 was above 109.17 ms and R/S ratio in V2 was above 0.28, forecasting a failed procedure.

Conclusions: The ECG predictors of failed ablation were characterized by RS duration and R/S ratio in V2.
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http://dx.doi.org/10.1155/2022/8160144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337924PMC
August 2022

Current Situation With Organ Donation and Transplantation in China: Application of Machine Learning.

Transplant Proc 2022 Aug 4. Epub 2022 Aug 4.

National Institute for Data Science in Health and Medicine, Xiamen University, Xiamen, China; Data Mining Research Center, Xiamen University, Xiamen, China; Xiang'an Hospital of Xiamen University, Xiamen, China. Electronic address:

Background: China ranks currently as one of the top countries for organ transplantation according to types, capacity, related research, quality, and quantity of procedures. However, the management systems struggle to support these practices. This study aims to review the current situation with organ donation and transplantation in China recently.

Methods: Data about organ donation and transplantation was collected by using web crawler technology from several news sections of representative portal websites in China. A total of 3475 documents about organ donation and transplantation were analyzed after data preprocessing. The documents were analyzed using machine learning to find out the relationship among the hot topics and their social popularity as well as to explore the dominant subtopics of the hot topics.

Results: The results of weighted correlation network analysis and documents clustering analysis identified 9 topics about organ donation and transplantation in China with a wide discussion: transplantation ethics, donor compensation, use of the remains, transportation guarantee of living organs, organ allocation, relevant national standards of organ donation and transplantation, legislation on organ donation, penalties for violation, and the Red Cross Society of China.

Conclusions: This study found out the main problems and proposed them for future discussion with organ donation and transplantation system in China: the lack of publicity and education and the difficulty of standardizing legislation about ethical issues arousing ethical problems; the need to understand the relationship and the standardization among costs, expenses, and compensation; and the lack of more support from the government and public to ensure regular organ donation and transplantation.
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http://dx.doi.org/10.1016/j.transproceed.2022.03.067DOI Listing
August 2022

Amide Proton Transfer Weighted MR Imaging for Predicting Meningioma Stiffness: A Feasibility Study.

J Magn Reson Imaging 2022 Aug 5. Epub 2022 Aug 5.

Department of Radiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Background: Stiffness of meningioma is an important factor affecting the surgical resection and the prognosis of patients.

Purpose: To examine the feasibility of APTw-magnetic resonance imaging (MRI) in evaluating meningioma stiffness.

Study Type: Retrospective.

Population: Seventy-one patient with meningiomas, 39 were male and 32 were female; the mean age was 51 ± 10 years.

Field Strength/sequence: 3.0T; Turbo-spin-echo T -weighted and Gd-T -weighted sequence; Turbo-spin-echo T -weighted sequence; 2D fat-suppressed, turbo-spin-echo APTw pulse sequence.

Assessment: The T WI signal intensity score, T WI signal intensity score, APTw , APTw , and APTw values were compared between soft, medium stiff and stiff meningiomas or non-stiff meningiomas and stiff meningiomas group.

Statistical Tests: Chi-square test, one-way ANOVA analysis, independent-samples t-test, intra-class correlation coefficient, rank-sum test, receiver operating characteristic curve analysis. P < 0.05 was considered statistically significant in all tests.

Results: APTw and APTw in the stiff group were significantly lower than that in the non-stiff group (2.79% ± 0.42% vs. 1.90% ± 0.60% and 3.20% ± 0.31% vs. 2.55% ± 0.61%). APTw and APTw in the stiff group were significantly lower than that in the medium stiff and soft groups (1.90% ± 0.60% vs. 2.69% ± 0.40% and 3.12% ± 0.32%, 2.55% ± 0.61% vs. 3.17% ± 0.33% and 3.39% ± 0.18%), APTw in the medium stiff group was significantly lower than in the soft group, there was no significant difference in APTw between the medium stiff and soft groups (P = 0.190). APTw showed the best diagnostic performance for evaluating meningioma stiffness with an area under the curve of 0.913, when the APTw was lower than 2.4%, the meningioma was defined as a stiff tumor, the sensitivity, specificity, and accuracy were 87.1%, 87.5%, and 85.9%, respectively.

Data Conclusion: APTw-MRI could be used to evaluate meningioma stiffness, with APTw having the best evaluative efficiency.

Level Of Evidence: 3 TECHNICAL EFFICACY: Stage 1.
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http://dx.doi.org/10.1002/jmri.28379DOI Listing
August 2022

Association Between Polymorphisms in Estrogen Receptor Genes and Depression in Women: A Meta-Analysis.

Front Genet 2022 19;13:936296. Epub 2022 Jul 19.

Department of Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

It is suggested that estrogen receptors (ERs) might be associated with the disproportionate vulnerability of women to depressive episodes. Several variants in ER-alpha (ERα) and ER-beta (ERβ) have been linked to depression, but the results were not consistent. Hence, we conducted a meta-analysis to evaluate the association between ERα/ERβ and depression in a cohort of women. A comprehensive literature search was performed in public databases. The genetic association between polymorphisms in Erα/ERβ and depression risk in a cohort of women was evaluated by odds ratios (ORs) and 95% confidence intervals (CIs). Cochran's Q test and the I index were used to evaluate heterogeneity. In total, 10 studies and 4 SNPs (rs2234693, rs9340799, rs4986938, rs1256049) were included in our meta-analysis. rs2234693 genotype was significantly associated with the risk of depression in women by dominant model (CC + CT vs TT, OR = 1.30, 95% CI: 1.09-1.55, = 0.0031), recessive model (CC vs CT + TT, OR = 1.64, 95% CI: 1.00-2.67, = 0.0478), additive model (CC vs TT, OR = 1.93, 95% CI: 1.12-3.35, = 0.0189) and allelic model (C vs T, OR = 1.24, 95% CI: 1.10-1.39, = 0.0003). For rs9340799, the frequencies of risk genotypes according to the dominant (GG + GA vs AA, OR = 1.47, 95% CI = 1.10-1.98, = 0.0096, I = 0%, = 0.43) and allelic (G vs A, OR = 1.33, 95% CI: 1.04-1.69, = 0.0236, I = 0%, = 0.39) models were significantly lower in women with depression than in controls within the Asian subgroup. For rs1256049, risk genotypes were significantly more frequent in depressed subjects than in controls under the dominant model (AA+ GA vs GG, OR = 1.62, 95% CI: 1.19-2.21, = 0.0024) and the allelic model (A vs G, OR = 1.35, 95% CI: 1.07-1.72, = 0.012) after sensitivity analysis by omitting one study which induce the heterogeneity. The current meta-analysis is the first and most comprehensive investigation of the association between ERs and depression in women, and the findings support the concept that ERs participate in the etiology of sex heterogeneity in depression.
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http://dx.doi.org/10.3389/fgene.2022.936296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343944PMC
July 2022

[Retrospective observation and analysis of autoimmune-related indicators in 317 patients with acute Omicron variant infection].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Jun;34(6):581-585

Department of Emergency, Tianjin First Center Hospital, Tianjin 300192, China. Corresponding author: Wang Yu, Email:

Objective: To compare the changes of clinical characteristics and immune-related indicators of patients with mild and moderate acute Omicron variant infection, and to evaluate the protective effect of coronavirus disease 2019 (COVID-19) vaccination.

Methods: The study retrospectively analyzed the clinical characteristics of 317 adult patients diagnosed with COVID-19 Omicron variant (B1.1.529) infection admitted to Tianjin First Central Hospital (Shuixi District) from January 22, 2022 to February 24, 2022. Demographic characteristics, vaccination status, underlying diseases, epidemiological characteristics, baseline data, and relevant laboratory test results on admission were collected, and the differences in clinical characteristics, especially the changes in immune-related indicators, between mild and moderate patients were compared and analyzed.

Results: Among the 317 adult patients with acute Omicron variant infection, the proportion of elderly, hypertension, diabetes, and cardiovascular or cerebrovascular diseases were significantly higher in moderate group (203 cases) than those of mild group (114 cases) [age ≥ 60 years old: 27.58% (56/203) vs. 9.65% (11/114), hypertension: 31.03% (63/203) vs. 19.30% (22/114), diabetes: 15.76% (32/203) vs. 7.89% (9/114), cardiovascular and cerebrovascular diseases: 11.33% (23/203) vs 0.88% (1/114), all P < 0.05]. The route of transmission was mainly through gatherings and the first symptoms were fever, dry cough, fatigue, sore throat, nasal congestion, runny nose and other flu symptoms; 19.30% (22/114) and 24.63% (50/203) of patients in mild and moderate groups were positive for the new coronavirus nucleic acid test, respectively, but the difference was not significant difference (P > 0.05). Inflammatory indicators in most mild and moderate patients were within normal range, such as white blood cell count (WBC), neutrophil ratio (NEU%), lymphocyte count (LYM), C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6), etc., suggesting that the acute phase of Omicron variant infection had not yet caused severe inflammatory storm, which might be related to the weakening of pathogenicity after vaccination and virus mutation. The proportion of patients with IL-6 > 7 ng/L in the mild group was significantly lower than that in the normal group [1.75% (2/114) vs. 6.40% (13/203), P < 0.05], suggesting that elevated IL-6 might be an important factor in evaluating indicators of disease severity. There was no significant difference in lymphocyte subsets between the two groups, but there were 12.90% (12/93) and 11.04% (17/154) of the patients in two groups, respectively, decreased in the proportion of helper T cells, and 18.28% (17/93) and 14.28% (22/154) of the patients had elevated CD4/CD8 ratio, suggesting that patients with Omicron variant infection had autoimmune system dysfunction, which might be related to disease progression and the occurrence of long-term autoimmune disease.

Conclusions: Serum IL-6 level may be used as a predictor for evaluating the severity of disease in patients with Omicron variant infection; after vaccination, inflammatory indicators in patients with acute Omicron variant infection were significantly reduced, but the long-term effects still require long-term follow-up observation.
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http://dx.doi.org/10.3760/cma.j.cn121430-20220418-00383DOI Listing
June 2022

The Value of Combined Detection of Megakaryocyte and Platelet Parameters for the Diagnosis of Primary Immune Thrombocytopenia.

Clin Appl Thromb Hemost 2022 Jan-Dec;28:10760296221106779

Department of Laboratory Medicine, the Second People's Hospital of Lianyungang City, Jiangsu Province, China.

To investigate the application value of bone marrow megakaryocyte count, the proportion of megakaryocytes at each stage, and the platelet parameter in the clinical diagnosis of primary immune thrombocytopenia (ITP). The megakaryocyte and platelet parameter level in 62 ITP and 40 control group patients were compared and analyzed. Linear correlation analysis, Pearson correlation analysis, and ROC curves were performed for the correlation between megakaryocytes and platelet parameters. Compared to the control group, the total number of megakaryocytes, the promegakaryocytes the granular megakaryocytes (GMeg), and naked megakaryocytes (NMeg), MPV, and P-LCR% in the ITP group increased. All differences were statistically significant (<0.05). While the proportion of platelet-producing megakaryocytes (PMeg), PLT, and PCT decreased in the ITP group. These differences were statistically significant ( 0.05). PLT was strongly positively correlated with PCT (r = 0.921, p<0.01). PCT was weakly positively with MPV (r = 0.309, p<0.05). MPV was positively correlated with P-LCR (r = 0.856, p<0.01). PDW was weakly positively correlated with P-LCR (r = 0.296, p<0.05) and Meg (r = 0.301, p<0.05), and negatively correlated with PMeg (r = -0.336, p<0.05). ROC curve analysis showed that PLT, PCT MPV and P-LCR% gave a high sensitivity(100.0%,81.0%,74.6%,90.5%,respectively.) and specificity (100.0%, 92.5%, 80.0%, 77.5%, respectively.) in diagnosis of ITP. The combined analysis of bone marrow megakaryocyte count, the proportion of megakaryocyte classification at each stage, and platelet parameters have an important reference value for auxiliary diagnosis of ITP.
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http://dx.doi.org/10.1177/10760296221106779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358600PMC
August 2022

Translesion synthesis of apurinic/apyrimidic site analogues by Y-family DNA polymerase Dbh from .

Acta Biochim Biophys Sin (Shanghai) 2022 May;54(5):637-646

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion synthesis (TLS) activity. To investigate the effect of the molecular structure of the AP site on the TLS efficiency of Dbh, a Y-family DNA polymerase from , a series of different AP site analogues (various spacers) are used to characterize the bypass efficiency. We find that not only the molecular structure and atomic composition but also the number and position of AP site analogues determine the TLS efficiency of Dbh. Increasing the spacer length decreases TLS activity. The TLS efficiency also decreases when more than one spacer exists on the DNA template. The position of the AP site analogues is also an important factor for TLS. When the spacer is opposite to the first incorporated dNTPs, the TLS efficiency is the lowest, suggesting that AP sites are largely harmful for the formation of hydrogen bonds. These results deepen our understanding of the TLS activity of Y-family DNA polymerases and provide a biochemical basis for elucidating the TLS mechanism in cells.
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http://dx.doi.org/10.3724/abbs.2022045DOI Listing
May 2022

Triglyceride-glucose index in the prediction of adverse cardiovascular events in patients with premature coronary artery disease: a retrospective cohort study.

Cardiovasc Diabetol 2022 Jul 29;21(1):142. Epub 2022 Jul 29.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China.

Background: Premature coronary artery disease (PCAD) has become more common in recent years and is often associated with poor outcomes. Triglyceride-glucose (TyG) index is a simple and reliable surrogate for insulin resistance (IR) and is an independent predictor of cardiovascular prognosis. However, the prognostic value of the TyG index in patients with PCAD remains uncertain. Thus, this study aimed to investigate the prognostic value and predictive performance of the TyG index in patients with PCAD.

Methods: A total of 526 young subjects (male < 45 years, female < 55 years) with angiographically proven CAD from January 2013 to December 2018 were included consecutively in this study. Their clinical and laboratory parameters were collected, and the TyG index was calculated as [Formula: see text]. The follow-up time after discharge was 40-112 months (median, 68 months; interquartile range, 49‒83 months). The primary endpoint was the occurrence of the major adverse cardiovascular events (MACE), defined as the composite of all-cause death, non-fatal myocardial infarction (MI), coronary artery revascularization, and non-fatal stroke.

Results: The TyG index was significantly associated with traditional cardiovascular risk factors and the Gensini score (GS). Kaplan-Meier survival (MACE-free) curves by tertiles of the TyG index showed statistically significant differences (log-rank test, p = 0.001). In the fully adjusted Cox regression model, the Hazard ratio (95% CI) of MACE was 2.17 (1.15-4.06) in tertile 3 and 1.45 (1.11-1.91) for per SD increase in the TyG index. Time-dependent ROC analyses of the TyG for prediction of MACE showed the area under the curves (AUC) reached 0.631 at 3 years, 0.643 at 6 years, and 0.710 at 9 years. Furthermore, adding TyG index to existing risk prediction model could improve outcome prediction [C-statistic increased from 0.715 to 0.719, p = 0.007; continuous net reclassification improvement (NRI) = 0.101, p = 0.362; integrated discrimination improvement (IDI) = 0.011, p = 0.017].

Conclusion: The TyG index is an independent predictor of MACE in patients with PCAD, suggesting that the TyG index has important clinical implications for risk stratification and early intervention of PCAD.
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http://dx.doi.org/10.1186/s12933-022-01576-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338459PMC
July 2022

Gadolinium-based ultra-small nanoparticles augment radiotherapy-induced T-cell response to synergize with checkpoint blockade immunotherapy.

Nanoscale 2022 Aug 11;14(31):11429-11442. Epub 2022 Aug 11.

Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China.

Radiotherapy suffers from its high-dose radiation-induced systemic toxicity and radioresistance caused by the immunosuppressive tumor microenvironment. Immunotherapy using checkpoint blocking in solid tumors shows limited anticancer efficacy due to insufficient T-cell infiltration and inadequate systemic immune responses. Activation and guiding of irradiation by X-ray (AGuIX) nanoparticles with sizes below 5 nm have entered a phase III clinical trial as efficient radiosensitizers. This study aimed to develop a unique synergistic strategy based on AGuIX-mediated radiotherapy and immune checkpoint blockade to further improve the efficiency for B16 tumor therapy. AGuIX exacerbated radiation-induced DNA damage, cell cycle arrest, and apoptosis on B16 cells. More importantly, it could efficiently induce the immunogenic cell death of irradiated B16 tumor cells, and consequently trigger the maturation of dendritic cells and activation of systemic T-cell responses. Combining AGuIX-mediated radiotherapy with programmed cell death protein 1 blockade demonstrated excellent synergistic therapeutic effects in both bilateral and metastatic B16 tumor models, as indicated by a significant increase in the infiltration of effector CD8 T cells and effective alleviation of the immunosuppressive tumor microenvironment. Our findings indicate that the synergy between radiosensitization and immunomodulation provides a new and powerful therapy regimen to achieve durable antitumor T-cell responses, which is promising for cancer treatment.
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http://dx.doi.org/10.1039/d2nr02620aDOI Listing
August 2022

Hollow nanospheres constructed by ultrafine few-layered MoS partially with amorphous fragments homogeneously incorporated in N-doped amorphous carbon for enhanced lithium storage performance.

J Colloid Interface Sci 2022 Jul 21;627:804-814. Epub 2022 Jul 21.

Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

The rational design of ultrathin and few-layered structures for three-dimensional MoS nanospheres is crucial for achieving attractive lithium-ion batteries (LIBs). Herein, hollow nanospheres constructed by ultrafine and few-layered MoS homogeneously incorporated in N-doped amorphous carbon (HUF-MoS/NC) have been successfully synthesized as high-performance anode for LIBs. Using Mo-glycerol spheres as templates and dopamine hydrochloride as coordination ligands, hollow Mo-glycerol-polydopamine precursors are formed with Mo-containing groups which are surrounded by organic carbon species. Consequently, the MoS is confined to the nanoscale and grows partially amorphous fragments while being uniformly embedded in NC. This unique architecture can not only hinder the substantial restacking between MoS interlayers, offering more active sites, but also vastly enhance the electrical conductivity and relieve the mechanical stress ascribed to volume changes. As a result, the HUF-MoS/NC composite anode exhibits excellent cyclic stability (980mAhg after 300 cycles at 0.2Ag) and superior rate performance (498mAhg at 5.0Ag) for LIBs.
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http://dx.doi.org/10.1016/j.jcis.2022.07.066DOI Listing
July 2022

A Novel Satellite PRN Code Assignment Method Based on Improved RLF Algorithm.

Sensors (Basel) 2022 Jul 25;22(15). Epub 2022 Jul 25.

China Academy of Space Technology (Xi'an), Xi'an 710100, China.

Low Earth Orbit (LEO) satellites have stronger received signals and more rapid geometry changes than Global Navigation Satellite System (GNSS) satellites, making them attractive for positioning, navigation, and timing (PNT) applications. Due to the low altitude, the LEO constellation requires more satellites to cover the entire globe and more Pseudo Random Noise (PRN) codes to realize Code Division Multiple Access (CDMA), which means greater receiver storage resources and receiver acquisition time. In this paper, different from the traditional methods that assign a unique PRN code to each satellite, we propose a novel method in which several satellites share the same PRN code, and simply demonstrate the feasibility and benefits of this method. To determine the minimum number of PRN codes needed for a constellation, we build a mathematical model. After the algorithm comparison, we improve the recursive largest first (RLF) algorithm so that it has a higher running speed and a smaller approximate optimal solution within a certain time period. By studying polar-orbiting and walker constellations, we find that if other satellite parameters remain the same, the higher the orbital altitude is, the more PRN codes are needed, and no matter what the orbital inclination is, the minimum number of PRN codes remains the same. Overall, it is feasible and meaningful for several satellites sharing the same PRN code to save storage resources and reduce the satellite acquisition time of the receiver. If this new technology is applied, the storage resources and the average satellite acquisition time of the receiver will be, at most, one-third of previous ones.
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http://dx.doi.org/10.3390/s22155538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331101PMC
July 2022

Color Design Decisions for Ceramic Products Based on Quantification of Perceptual Characteristics.

Sensors (Basel) 2022 Jul 20;22(14). Epub 2022 Jul 20.

School of Design and Art, Shaanxi University of Science and Technology, Xi'an 710021, China.

The appearance characteristics of ceramic color are an important factor in determining the user's aesthetic perception of the product. Given the problem that ceramic color varies and the user's visual sensory evaluation of color is highly subjective and uncertain, a method of quantifying ceramic color characteristics based on the Back Propagation (BP) neural network algorithm is proposed. The semantic difference method and statistical method were used to obtain quantified data from ceramic color perceptual semantic features and were combined with a neural network to study the association between ceramic color features and user perceptual-cognitive evaluation. A BP neural network was used to build a ceramic color perceptual semantic mapping model, using color semantic quantified values as the input layer, color L, A, and B component values as the output layer, and model training to predict the sample. The output color L, A, and B components are used as the input layer and the color scheme was designed. The above method can effectively solve the mapping problem between the appearance characteristics of ceramic color and perceptual semantics and provide a decision basis for ceramic product color design. The case application of color design of daily-use ceramic products was conducted to verify the effectiveness and feasibility of the quantitative research method of ceramic color imagery.
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http://dx.doi.org/10.3390/s22145415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322515PMC
July 2022

Apoptosis-Related Gene-Mediated Cell Death Pattern Induces Immunosuppression and Immunotherapy Resistance in Gastric Cancer.

Front Genet 2022 5;13:921163. Epub 2022 Jul 5.

Department of Gastrointestinal Surgery, Maoming People's Hospital, Maoming, China.

Apoptosis is a type of cell death, which can produce abundant mediators to modify the tumor microenvironment. However, relationships between apoptosis, immunosuppression, and immunotherapy resistance of gastric cancer (GC) remain unclear. Gene expression data and matching clinical information were extracted from TCGA-STAD, GSE84437, GSE34942, GSE15459, GSE57303, ACRG/GSE62254, GSE29272, GSE26253, and IMvigor210 datasets. A consensus clustering analysis based on six apoptosis-related genes (ARGs) was performed to determine the molecular subtypes, and then an apoptosisScore was constructed based on differentially expressed and prognostic genes between molecular subtypes. Estimate R package was utilized to calculate the tumor microenvironment condition. Kaplan-Meier analysis and ROC curves were performed to further confirm the apoptosisScore efficacy. Based on six ARGs, two molecular subgroups with significantly distinct survival and immune cell infiltration were identified. Then, an apoptosisScore was built to quantify the apoptosis index of each GC patient. Next, we investigated the correlations between the clinical characteristics and apoptosisScore using logistic regression. Multivariate Cox analysis shows that low apoptosisScore was an independent predictor of poor overall survival in TCGA and ACRG datasets, and was associated with the higher pathological stage. Meanwhile, low apoptosisScore was associated with higher immune cell, higher ESTIMATEScore, higher immuneScore, higher stromalScore, higher immune checkpoint, and lower tumorpurity, which was consistent with the "immunity tidal model theory". Importantly, low apoptosisScore was sensitive to immunotherapy. In addition, GSEA indicated that several gene ontology and Kyoto Encyclopedia of Genes and Genomes items associated with apoptosis, several immune-related pathways, and JAK-STAT signal pathway were considerably enriched in the low apoptosisScore phenotype pathway. Our findings propose that low apoptosisScore is a prognostic biomarker, correlated with immune infiltrates, and sensitivity to immunotherapy in GC.
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http://dx.doi.org/10.3389/fgene.2022.921163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295743PMC
July 2022

ECM-mimetic immunomodulatory hydrogel for methicillin-resistant -infected chronic skin wound healing.

Sci Adv 2022 07 8;8(27):eabn7006. Epub 2022 Jul 8.

Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China.

The treatment of difficult-to-heal wounds remains a substantial clinical challenge due to deteriorative tissue microenvironment including the loss of extracellular matrix (ECM), excessive inflammation, impaired angiogenesis, and bacterial infection. Inspired by the chemical components, fibrous structure, and biological function of natural ECM, antibacterial and tissue environment-responsive glycopeptide hybrid hydrogel was developed for chronic wound healing. The hydrogel can facilitate the cell proliferation and macrophage polarization to M2 phenotype, and show potent antibacterial efficacy against both Gram-negative and Gram-positive bacteria. Significantly, the glycopeptide hydrogel accelerated the reconstruction of methicillin-resistant (MRSA)-infected full-thickness diabetic and scalding skin by orchestrating a pro-regenerative response indicated by abundant M2-type macrophages, attenuated inflammation, and promoted angiogenesis. Collectively, ECM-mimetic and immunomodulatory glycopeptide hydrogel is a promising multifunctional dressing to reshape the damaged tissue environment without additional drugs, exogenous cytokines, or cells, providing an effective strategy for the repair and regeneration of chronic cutaneous wounds.
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http://dx.doi.org/10.1126/sciadv.abn7006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269894PMC
July 2022

Economic benefit evaluation of water resources allocation in transboundary basins based on particle swarm optimization algorithm and cooperative game model-A case study of Lancang-Mekong River Basin.

PLoS One 2022 19;17(7):e0265350. Epub 2022 Jul 19.

School of Business, Hohai University, Nanjing, China.

The present work aims to find the optimal solution of Nash Equilibrium (NE) in the traditional Game Theory (GT) applied to water resources allocation. Innovatively, this paper introduces Particle Swarm Optimization (PSO) into GT to propose a cooperative game model to solve the NE problem. Firstly, the basic theory of the PSO algorithm and cooperative game model is described. Secondly, the PSO-based cooperative game model is explained. Finally, the PSO-based cooperative game model is compared with the Genetic Algorithm (GA) to test the performance. Besides taking the countries in Lancang Mekong River Basin as the research object, this paper discusses each country's water consumption and economic benefits under different cooperation patterns. Then, a series of improvement measures and suggestions are put forward accordingly. The results show that the average server occupancy time of the PSO-based cooperative game model is 78.46% lower than that of GA, and the average waiting time is 79.24% lower than that of the GA. Thus, the model reported here has higher computational efficiency and excellent performance than the GA and is more suitable for the current study. In addition, the multi-country cooperation mode can obtain more economic benefits than the independent water resource development mode. This model can quickly find the optimal combination of 16 cooperation modes and has guiding significance for maximizing the benefits of cross-border water Resource Utilization. This research can provide necessary technical support to solve the possible contradictions and conflicts between cross-border river basin countries and build harmonious international relations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265350PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295979PMC
July 2022

Lentinan Enhances the Function of Oxaliplatin on the Esophageal Tumors by Persuading Immunogenic Cell Death.

Comput Math Methods Med 2022 8;2022:2296574. Epub 2022 Jul 8.

Department of Basic Medicine, Changzhi Medical College, Changzhi, Shanxi Province, China.

Objective: The focus of this research was to look at the effects of the combination of the lentinan (LNT) and oxaliplatin (Oxa) on the apoptosis of human esophageal cancer cells, as well as the underlying mechanism.

Methods: LNT and Oxa were used to treat EC-109 human esophageal cancerous cells at various doses, and the cell survival rate was measured using the Cell Counting Kit-8 (CCK-8) assay. In addition, 24 h after treatment of EC-109 cells with a combination of LNT and Oxa, flow cytometry was used to analyze their apoptotic effect on these cells. Additionally, LNT on EC-109 cell apoptotic upshot was assessed via measuring the consequence of LNT on the mRNA and protein expression levels pertaining to immunogenic cell death factors CALR, HSP90, and HSP70 by qPCR (quantitative real-time polymerase chain reaction) and western blot analysis, correspondingly.

Results: Cell proliferation was inhibited only when EC-109 cells were added with LNT at 1,200 g/mL to the maximum concentrations, but the combination of LNT and Oxa at a low dose (800 g/mL and 20 M, respectively) significantly increased their sensitivity to Oxa and reduced their proliferation ( < 0.05), and their apoptosis was significantly increased by LNT ( < 0.05). The immunogenic cell death-related genes CALR, HSP90, and HSP70 had dramatically enhanced mRNA and protein expression levels after therapy with a combination of LNT and Oxa ( < 0.05).

Conclusion: These data imply that LNT increases the susceptibility of esophageal cancerous cells to Oxa by driving EC-109 cells to display immunogenic death. Therefore, LNT combined with Oxa may be an effective method in esophageal cancer management.
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http://dx.doi.org/10.1155/2022/2296574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286936PMC
July 2022

Pricing Israeli Option with Time-changed Compensation by an FFT-Based High-order Multinomial Tree in Lévy Markets.

Comput Intell Neurosci 2022 29;2022:9682292. Epub 2022 Jun 29.

School of Economics and Management, Southeast University, Nanjing 210096, China.

The problem for pricing the Israel option with time-changed compensation was studied based on the high-order recombined multinomial tree by using a fast Fourier transform to approximate a Lévy process. First, the Lévy option pricing model and Fourier transform are introduced. Then, a network model based on FFT (Markov chain) is presented. After that, an FFT-based multinomial tree construction method is given to solve the problem of difficult parameter estimation when approximating the Lévy process with high-order multinomial trees. It is proved that the discrete random variables corresponding to the multinomial tree converge to the Lévy-distributed continuous random variable. Next, an algorithm based on a reverse recursion algorithm for pricing the Israel option with time-changed compensation was presented. Finally, an example was illustrated, and the relationship between the price of the Israel option and the time-changed compensation was discussed. The results show that the method of constructing a high-order recombined multinomial tree based on FFT has very high calculation precision and calculation speed, which can solve the problem of traditional risk-neutral multinomial tree construction, and it is a promising pricing method for pricing Israel options.
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http://dx.doi.org/10.1155/2022/9682292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259298PMC
July 2022

Immune Infiltration and N(6)-Methyladenosine ncRNA Isoform Detection in Acute Lung Injury.

J Oncol 2022 30;2022:3922299. Epub 2022 Jun 30.

Department of Laboratory Medicine, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, China.

Acute lung injury (ALI) is a severe form of sepsis that is associated with a high rate of morbidity and death in critically ill individuals. The emergence of ALI is the result of several factors at work. Case mortality rates might range from 40% to 70%. Researchers have discovered that epigenetic alterations are important in the pathophysiology of ALI and that using epigenetic inhibitors may help reduce symptoms. In embryonic development, circadian rhythm, the cell cycle, and cancer, methylation of m6A seems to be relevant all along the way. According to recent research, posttranscriptional methylation is a key player in the development of alveolar lymphoma. In this study, we clustered ALI based on m6A-related factors, analyzed different classes of immune cell enrichment and inflammatory cytokine expression, screened clustered differential genes for ALI to construct coexpression networks, screened key ALI genes potentially regulated by m6A modifications, and then typed the disease based on key genes to compare the consistency of different clustering results. Our findings have revealed a hitherto undiscovered prognostic sign and a therapeutic target for ALI therapy in m6A and immune invading cells, respectively.
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http://dx.doi.org/10.1155/2022/3922299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262529PMC
June 2022

Relationship between MMP-9 Gene Polymorphism and Intracranial Aneurysm.

Cell Mol Biol (Noisy-le-grand) 2022 Jan 30;68(1). Epub 2022 Jan 30.

Department of Neurosurgery, Zhangzhou Hospital, Fujian Medical University, Zhangzhou, 363000, China.

Matrix metalloproteinase-9 (MMP-9) is a gelatinase, which is a member of the MMPs family. We know that MMP-9 is not only an important gelatinase to regulate the extracellular matrix balance, but also one of the most closely related proteases to the pathogenesis of intracranial aneurysm. The purpose of this study is to investigate the relationship between MMP-9 gene polymorphism and intracranial aneurysm. In this paper, 98 patients who were admitted to a hospital from 2018 to 2019 were selected as the experimental group and the control group according to the relevant standards of intracranial aneurysms. The MMP-9 positive and MMP-9 absorbance values between the two groups were compared, so as to determine the concentration of MMP-9 between the two groups. In addition, the gene distribution and gene frequency analysis of the C-1562T promoter region of MMP-9 were carried out. The results showed that in the control group, the gene distribution frequency of CC type was 67% that of CT type was 31% that of TT type was 2%, that of the experimental group was 52%, and that of CT type was 44%. The results showed that there was a correlation between MMP-9 gene polymorphism and intracranial aneurysm.
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http://dx.doi.org/10.14715/cmb/2022.68.1.3DOI Listing
January 2022

Utilization of AlphaFold2 to Predict MFS Protein Conformations after Selective Mutation.

Int J Mol Sci 2022 Jun 29;23(13). Epub 2022 Jun 29.

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, China.

The major facilitator superfamily (MFS) is the largest secondary transporter family and is responsible for transporting a broad range of substrates across the biomembrane. These proteins are involved in a series of conformational changes during substrate transport. To decipher the transport mechanism, it is necessary to obtain structures of these different conformations. At present, great progress has been made in predicting protein structure based on coevolutionary information. In this study, AlphaFold2 was used to predict different conformational structures for 69 MFS transporters of after the selective mutation of residues at the interface between the N- and C-terminal domains. The predicted structures for these mutants had small RMSD values when compared to structures obtained using X-ray crystallography, which indicates that AlphaFold2 predicts the structure of MSF transporters with high accuracy. In addition, different conformations of other transporter family proteins have been successfully predicted based on mutation methods. This study provides a structural basis to study the transporting mechanism of the MFS transporters and a method to probe dynamic conformation changes of transporter family proteins when performing their function.
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http://dx.doi.org/10.3390/ijms23137235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266783PMC
June 2022

Perioperative stroke in patients undergoing spinal surgery: a retrospective cohort study.

BMC Musculoskelet Disord 2022 Jul 8;23(1):652. Epub 2022 Jul 8.

Department of Neurology, Beijing Jishuitan Hospital, Beijing, China.

Background: The incidence of perioperative stroke following spinal surgery, including ischemic and hemorrhagic stroke, has not been fully investigated in the Chinese population. Whether specific spinal or emergency/elective procedures are associated with perioperative stroke remains controversial. This study aimed to investigate the incidence of perioperative stroke, health economic burden, clinical outcomes, and associated risk factors.

Method: A retrospective cohort study using an electronic hospital information system database was conducted from Jan 1, 2015, to Jan 1, 2021, in a tertiary hospital in China. Patients aged ≥18 years who had undergone spinal surgery were included in the study. We recorded patient demographics, comorbidities, and health economics data. Clinical outcomes included perioperative stroke during hospitalization and associated risk factors. The patients' operative data, anesthetic data, and clinical manifestations were recorded.

Result: A total of 17,408 patients who had undergone spinal surgery were included in this study. Twelve patients had perioperative stroke, including seven ischemic stroke (58.3%) and five hemorrhagic stroke (41.7%). The incidence of perioperative stroke was 0.07% (12/17,408). In total, 12 stroke patients underwent spinal fusion. Patients with perioperative stroke were associated with longer hospital stay (38.33 days vs. 9.78 days, p < 0.001) and higher hospital expenses (RMB 175,642 vs. RMB 81,114, p < 0.001). On discharge, 50% of perioperative patients had severe outcomes. The average onset time of perioperative stroke was 1.3 days after surgery. Stroke history (OR 146.046, 95% CI: 28.102-759.006, p < 0.001) and hyperlipidemia (OR 4.490, 95% CI: 1.182-17.060, p = 0.027) were associated with perioperative stroke.

Conclusion: The incidence of perioperative stroke of spinal surgery in a tertiary hospital in China was 0.07%, with a high proportion of hemorrhagic stroke. Perioperative stroke patients experienced a heavy financial burden and severe outcomes. A previous stroke history and hyperlipidemia were associated with perioperative stroke.
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http://dx.doi.org/10.1186/s12891-022-05591-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264537PMC
July 2022

Cervical Cancer Cells-Derived Extracellular Vesicles Containing microRNA-146a-5p Affect Actin Dynamics to Promote Cervical Cancer Metastasis by Activating the Hippo-YAP Signaling Pathway WWC2.

J Oncol 2022 28;2022:4499876. Epub 2022 Jun 28.

Department of Laboratory Medicine, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, China.

Application of extracellular vesicles (EVs) for cancer treatment has been well-documented. We probed into the potential role of cervical cancer cells-secreted EVs by transferring miR-146a-5p in cervical cancer. After characterization of miR-146a-5p expression in clinical cervical cancer tissue samples, gain- and loss-of-function experiments were implemented to test the effect of miR-146a-5p on the invasion, epithelial-mesenchymal transition (EMT), and anoikis in cervical cancer cells. EVs were isolated from high-metastatic cervical cancer cells, after which their effects on the malignant behaviors of low-metastatic cervical cancer cells were assessed in a co-culture system. Luciferase assay was implemented to validate the putative binding relationship between miR-146a-5p and WWC2, followed by further investigation of downstream pathway (Hippo-YAP). Finally, nude mouse lung metastasis model was developed for validation. miR-146a-5p was elevated in cervical cancer tissues and high miR-146a-5p expression promoted the metastatic potential of cervical cancer cells through enhancing their invasiveness and anoikis resistance, and inducing EMT. Furthermore, miR-146a-5p carried by EVs secreted by highly metastatic cervical cancer cells could promote the metastasis of low-metastatic cervical cancer cells. Mechanistically, miR-146a-5p targeted WWC2 to activate YAP, by which it inhibited the phosphorylation of cofilin, and promoted the process of cofilin-mediated depolymerization of F-actin to G-actin. data demonstrated that EVs-carried miR-146a-5p promoted tumor metastasis through the WWC2/YAP axis. Cancer-derived EVs delivered pro-metastatic miR-146a-5p to regulate the actin dynamics in cervical cancer, thereby leading to cancer metastasis. This experiment highlighted an appealing therapeutic modality for cervical cancer.
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http://dx.doi.org/10.1155/2022/4499876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256433PMC
June 2022

PtoNF-YC9-SRMT-PtoRD26 module regulates the high saline tolerance of a triploid poplar.

Genome Biol 2022 07 7;23(1):148. Epub 2022 Jul 7.

Key Laboratory for Bio-resources and Eco-environment of Ministry of Education, College of Life Science, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu, 610065, China.

Background: Sensing and responding to stresses determine the tolerance of plants to adverse environments. The triploid Chinese white poplar is widely cultivated in North China because of its adaptation to a wide range of habitats including highly saline ones. However, its triploid genome complicates any detailed investigation of the molecular mechanisms underlying its adaptations.

Results: We report a haplotype-resolved genome of this triploid poplar and characterize, using reverse genetics and biochemical approaches, a MYB gene, SALT RESPONSIVE MYB TRANSCRIPTION FACTOR (SRMT), which combines NUCLEAR FACTOR Y SUBUNIT C 9 (PtoNF-YC9) and RESPONSIVE TO DESICCATION 26 (PtoRD26), to regulate an ABA-dependent salt-stress response signaling. We reveal that the salt-inducible PtoRD26 is dependent on ABA signaling. We demonstrate that ABA or salt drives PtoNF-YC9 shuttling into the nucleus where it interacts with SRMT, resulting in the rapid expression of PtoRD26 which in turn directly regulates SRMT. This positive feedback loop of SRMT-PtoRD26 can rapidly amplify salt-stress signaling. Interference with either component of this regulatory module reduces the salt tolerance of this triploid poplar.

Conclusion: Our findings reveal a novel ABA-dependent salt-responsive mechanism, which is mediated by the PtoNF-YC9-SRMT-PtoRD26 module that confers salt tolerance to this triploid poplar. These genes may therefore also serve as potential and important modification targets in breeding programs.
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http://dx.doi.org/10.1186/s13059-022-02718-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264554PMC
July 2022

Efficacy of combination of endo-xylanase and xylan-debranching enzymes in improving application of cereal bran in piglet diet.

Anim Biosci 2022 Jun 30. Epub 2022 Jun 30.

College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

Objective: This study was aimed to explore the efficacy of combination of endo-xylanase (Xyn) and xylan-debranching enzymes (arabinofuranosidase, Afd and feruloyl esterase, FE) in improving utilization of bran in piglet diet.

Methods: In vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd and FE, concurrent with their effect on degradation of arabinoxylan (Abx) in bran. In vivo experiment was then implemented by allocating two hundred and seventy 35-d-old postweaning piglets into 3 groups (6 replicates/group), which received bran-containing diet supplemented with Xyn (1600 U/kg) or its combination with Afd (0.8 U/kg) and FE (4 U/kg) or without enzyme.

Results: Both Xyn, Afd and FE are relatively stable against the changes in temperature and pH value. Combining Xyn with Afd and FE had a superiority (p<0.05) over Xyn alone and its combination with Afd or FE in promoting (p<0.05) degradation of Abx in different brans. Combined treatment with Xyn, Afd and FE was more beneficial than Xyn alone to induce increasing trends (p<0.10) of average daily gain, final body weight and feed efficiency of piglets fed bran-containing diet. Moreover, combination of Xyn, Afd and FE showed advantages (p<0.05) over Xyn alone in causing reductions (p<0.05) in diarrhea rate and cecal pH value, concurrent with increases (p<0.05) in cecal and colonic acetic acid and total volatile fatty acid concentrations, as well as cecal butyric acid concentration of piglets fed bran-containing diet.

Conclusion: Combining Xyn with Afd and FE was more beneficial than Xyn alone in promoting degradation of Abx in bran, along with growth performance and intestinal volatile fatty acid profile of piglets received bran-containing diet. Thereby, combination of Xyn, Afd and FE had a superior efficacy relative to Xyn alone in improving application of cereal bran in piglet diet.
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http://dx.doi.org/10.5713/ab.21.0534DOI Listing
June 2022
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