Publications by authors named "Weina Wang"

105 Publications

Determining the function of LvSmad3 on Litopenaeus vannamei in response to acute low temperature stress.

Dev Comp Immunol 2021 Jul 22;125:104209. Epub 2021 Jul 22.

Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, 510631, PR China. Electronic address:

Smad3 is a key mediator of the canonical TGF-β signaling pathway and plays an important role in TGF-β1-mediated transcriptional regulation. However, the function of Smad3 in crustaceans such as shrimp, is still poorly understood and needs to be further explored. We characterized Litopenaeus vannamei Smad3 (LvSmad3) and its biological functions were investigated in response low temperature stress. Full-length LvSmad3 cDNA was 2341bp and contained an open reading frame (ORF) of 1326 bp that encoded a 441 amino acid long protein, with a predicted molecular mass of 48.35 kDa. Phylogenetic analysis revealed that LvSmad3 has a high degree of similarity with other known species. LvSmad3 mRNA was detected in all the tested tissues and highest transcription occurred mostly in gills. Further research showed that suppressing the expression of Smad3 could reduce ROS production, DNA damage and the apoptosis rate in shrimp hemocyte under low temperature compared with the dsGFP group. Thus, we speculated that Smad3 could promote the apoptosis of hemocytes. We confirmed that Smad3 could inhibit apoptosis in the hepatopancreas by suppressing the expression of pro-apoptotic genes. Taken together, the silencing of Smad3 can reduce ROS production induced by low temperature stress, weaken the damage to hemocytes and the hepatopancreas by inhibit the apoptosis.
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http://dx.doi.org/10.1016/j.dci.2021.104209DOI Listing
July 2021

Metabolic alterations of the dorsolateral prefrontal cortex in sleep-related hypermotor epilepsy: A proton magnetic resonance spectroscopy study.

J Neurosci Res 2021 Jun 16. Epub 2021 Jun 16.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, China.

Sleep-related hypermotor epilepsy (SHE) is a focal epilepsy whose neurobiological underpinnings remain poorly understood. The present study aimed to identify possible neurochemical alterations in the dorsolateral prefrontal cortex (DLPFC) in participants with SHE using proton magnetic resonance spectroscopy ( H MRS). Thirty-nine participants with SHE (mean age, 30.7 years ± 11.3 [standard deviation], 24 men) and 59 controls (mean age, 29.4 years ± 10.4, 29 men) were consecutively and prospectively recruited and underwent brain magnetic resonance imaging and H MRS in the bilateral DLPFCs. Brain concentrations of metabolites, including N-acetyl aspartate (NAA), myo-inositol (mI), choline, creatine, the sum of glutamate and glutamine, glutathione (GSH) and γ-aminobutyric acid, were estimated with LCModel and corrected for the partial volume effect of cerebrospinal fluid using tissue segmentation. ANCOVA analyses revealed lower concentration of NAA in the left DLPFC in participants with SHE compared with controls. A significant difference of NAA concentration between DLPFC in the two hemispheres (left > right) was observed only in the control group. We further confirmed a higher GSH concentration in men than in women in SHE participants, which probably indicates that men are more susceptible to this disease. The mI concentration in the right DLPFC was negatively correlated with epilepsy duration. This study demonstrates that DLPFC is an important brain region involved in the pathophysiology of SHE, in which both neurons and astrocytes appear impaired, and the elevated GSH level may suggest an abnormality related to oxidative stress.
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http://dx.doi.org/10.1002/jnr.24866DOI Listing
June 2021

Mesh Total Generalized Variation for Denoising.

IEEE Trans Vis Comput Graph 2021 Jun 11;PP. Epub 2021 Jun 11.

Recent study has shown that the Total Generalized Variation (TGV) is highly effective in preserving sharp features as well as smooth transition variations for image processing tasks. However, currently there is no existing work that is suitable for applying TGV to 3D data, in particular, triangular meshes. In this paper, we develop a novel framework for discretizing second-order TGV on triangular meshes. Further, we propose a TGV-based variational method for the denoising of face normal fields on triangular meshes. The TGV regularizer in our method is composed of a first-order term and a second-order term, which are automatically balanced. The first-order term allows our TGV regularizer to locate and preserve sharp features, while the second-order term allows to recognize and recover smoothly curved regions. To solve the optimization problem, we introduce an efficient iterative algorithm based on variable-splitting and augmented Lagrangian method. Extensive results and comparisons on synthetic and real scanning data validate that the proposed method outperforms the state-of-the-art visually and numerically.
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http://dx.doi.org/10.1109/TVCG.2021.3088118DOI Listing
June 2021

Potential roles for microRNAs in facilitating physiological adaptation to low-temperature stress in Penaeus vannamei.

J Fish Dis 2021 Aug 1;44(8):1191-1200. Epub 2021 Jun 1.

Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, China.

Water temperature is one of the most common physiological stressors in aquaculture. Previous studies demonstrate that organisms require miRNA activity for survival in various unfavourable environmental conditions. However, the detailed role of miRNA in response to low-temperature stress is still unclear. This study was conducted to construct a comprehensive miRNA dataset for the Penaeus vannamei after low-temperature stress. A total of 329 known miRNAs and 60 putative novel miRNAs were identified. Among them, 17 miRNAs were identified with the most significant differences, and they were found to be involved in stimulation or stress processes. The main enriched target pathways of the 17 miRNAs were the Hippo signalling pathway, autophagy, apoptosis and MAPK signalling. In addition, all the 17 miRNAs identified were up-regulated, suggesting that miRNA by inhibiting the expression of target genes constitutes an effective strategy for Penaeus vannamei to cope with low-temperature stress. The 35-putative target of the 17 miRNAs was related to apoptosis and autophagy-related proteins, such as Pxt, DRAM2, cytochrome c, ATG2B, JNK, ATG4 and API5. The analysis of miRNA expression profiles contributes to the understanding of the molecular mechanisms of low-temperature tolerance in Penaeus vannamei. This study's findings enrich current miRNA resources and offer the possibility to validate the involvement of 17 miRNAs in the response of shrimp to low-temperature stress.
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http://dx.doi.org/10.1111/jfd.13379DOI Listing
August 2021

Gray matter abnormalities in Tourette Syndrome: a meta-analysis of voxel-based morphometry studies.

Transl Psychiatry 2021 05 14;11(1):287. Epub 2021 May 14.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, 610041, China.

Tourette syndrome (TS) is a neurobehavioral disorder for which the neurological mechanism has not been elucidated. Voxel-based morphometry (VBM) studies have revealed abnormalities in gray matter volume (GMV) in patients with TS; however, consistent results have not been obtained. The current study attempted to provide a voxel wise meta-analysis of gray matter changes using seed-based d mapping (SDM). We identified ten relevant studies that investigated gray matter alterations in TS patients and performed a meta-analysis using the SDM method to quantitatively estimate regional gray matter abnormalities. Next, we examined the relationships between GMV abnormalities and demographic and clinical characteristics. Our results demonstrated that TS patients had smaller GMV in the bilateral inferior frontal gyri and greater GMV in the cerebellum, right striatum (putamen), and bilateral thalami (pulvinar nucleus) than healthy controls. A meta-regression analysis did not identify correlations between GMV changes and demographic or clinical variables. This meta-analysis confirmed significant and consistent GMV changes in several brain regions of TS patients, primarily in the cortico-striato-thalamo-cortical network.
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http://dx.doi.org/10.1038/s41398-021-01394-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121885PMC
May 2021

Preparation of carbon dots-hematite quantum dots-loaded hydroxypropyl cellulose-chitosan nanocomposites for drug delivery, sunlight catalytic and antimicrobial application.

J Photochem Photobiol B 2021 Jun 24;219:112201. Epub 2021 Apr 24.

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

In this project, we studied the thermal and chemical method for the synthesis of carbon dots (CDs)/Hematite (α-FeO) quantum dots and the preparation of hydroxypropyl cellulose cross-linked chitosan (HPCCS) and ulvan (UN) was performed by chemical method. Carbon dots/α-FeO quantum dots with size distribution of 3-5 nm were completely encapsulated in the HPCCS/UN NPs to obtain composites, which indicated unique characteristics with respect to antimicrobial, pH-responsive and optical properties. The CDs-HQDs/HPCCS/UN nanocomposites exhibited a single-excitation (440 nm), dual-emission fluorescence property (505 nm and 628 nm for green and red light from CDs-HQDs and HPCCS/UN NPs). The nanocomposites played as a pH-responsive drug delivery process to release ulvan at a fast rate in pH 7.4 buffer solution but at a slow rate in low pH solutions. The CDs-HQDs/HPCCS/UN nanocomposites gained the highest photocatalytic activity for degrading 4-chlorophenol (4-CPh) as a pollutant (>98% during 70 min under sunlight irradiation). Moreover, the nanocomposites indicated great inhibitory influences towards bacterial and fungal.
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http://dx.doi.org/10.1016/j.jphotobiol.2021.112201DOI Listing
June 2021

miR-151 Affects Low-Temperature Tolerance of by Modulating Autophagy Under Low-Temperature Stress.

Front Cell Dev Biol 2021 9;9:595108. Epub 2021 Apr 9.

Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Sciences, South China Normal University, Guangzhou, China.

MicroRNAs (miRNAs) play key roles in many physiologic and pathologic processes, including autophagy. Autophagy is cellular in an emergency response mechanism of environment stress, but their complex molecular regulatory mechanism under low-temperature stress is largely unknown in shrimp, especially miRNA-mediated regulation of autophagy in low-temperature tolerance. In this article, a shrimp and miRNA pva-miR-151 cooperation in response to low-temperature stress has been reported. Pva-miR-151 showed expression patterns opposite to target under low-temperature stress. The pva-miR-151 targets the 3'-UTR region of , regulate the formation of autophagosome, which contribute to the degradation and recycling of damaged organelles. In addition, the low-temperature tolerance was correlated positively with autophagy in shrimp. Silenced pva-miR-151 increased sensitivity to low-temperature stress, whereas overexpression pva-miR-151 decreased the expression of PvTOR and p-TOR and increased tolerance to low-temperature stress by improving the formation of autophagosome and total hemocyte count. In addition, the TOR activator 3BDO can partially rescue autophagy induced by overexpression of pva-miR-151; these results indicate that miR-151 was necessary for the low-temperature tolerance in shrimp. Taken together, we provide a novel strategy and mechanism for shrimp breeding to improve shrimp low-temperature tolerance.
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http://dx.doi.org/10.3389/fcell.2021.595108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064728PMC
April 2021

Quantitative 1H-MRS reveals metabolic difference between subcategories of malformations of cortical development.

Neuroradiology 2021 Mar 23. Epub 2021 Mar 23.

Department of Radiology, West China Hospital of Sichuan University, No. 37 Guo Xue lane, Chengdu, 610041, Sichuan, China.

Purpose: To figure out the spectra features of malformations of cortical development (MCDs) and the differences between MCDs subcategories.

Methods: Twenty patients and 18 controls were studied. The patients included two subcategories: disorders of migration (DOM) and postmigration (DOPM). Spectra of patients were acquired from both the lesion and the normal-appearing contralateral side (NACS), and they were compared to those of the controls obtained from the frontal lobe.

Results: Compared to the controls, a decreased NAA (P = 0.002) was identified in MCDs. After dividing the MCDs into the DOM and DOPM, we found that NAA reduction was only notable in the DOM (P = 0.007). Moreover, Ins and Cr of the DOPM were higher than those of the controls (P = 0.017 and 0.013) and the DOM (P = 0.027 and 0.001). Compared to the NACS, a decreased NAA (P = 0.042) and an increased Ins (P = 0.039) were identified in the lesion of MCDs. After dividing the MCDs into the DOM and DOPM, we found no significant differences in the DOM, but Ins, Cr, and Glx of the lesion were higher than those of the NACS (P = 0.007, 0.005 and 0.047) in the DOPM. In addition, we found that Cr and Glx correlated positively to the seizure frequency (P = 0.003 and 0.016).

Conclusion: Decreased NAA was the prominent abnormality confirmed in MCDs. Spectra of different MCDs subcategories were different: the DOM was characterized by decreased NAA, while the DOPM was characterized by increased Ins.
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http://dx.doi.org/10.1007/s00234-021-02694-yDOI Listing
March 2021

TIPE1 Inhibits Hepatic Steatosis, Inflammation, and Fibrosis by Suppressing Polyubiquitination of ASK1.

Hepatology 2021 Mar 13. Epub 2021 Mar 13.

Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University.

Characterized by hepatocyte steatosis, inflammation, and fibrosis, nonalcoholic steatohepatitis (NASH) is a complicated process that contributes to end-stage liver disease and, eventually, hepatocellular carcinoma (HCC). Tumor necrosis factor alpha (TNF-α)-induced protein 8-like 1 (TIPE1), a new member of the TNF-α-induced protein 8 family, has been explored in immunology and oncology research, but little is known about its role in metabolic diseases. Here, we revealed that hepatocyte-specific deletion of TIPE1 exacerbated diet-induced hepatic steatosis, inflammation, and fibrosis as well as systemic metabolic disorders during NASH pathogenesis. Conversely, hepatocyte-specific overexpression of TIPE1 dramatically prevented the progression of these abnormalities. Mechanically, TIPE1 directly interacted with apoptosis signal-regulating kinase 1 (ASK1) to suppress its TNF receptor-associated factor 6 (TRAF6)-catalyzed polyubiquitination activation upon metabolic challenges, thereby inhibiting downstream c-Jun N-terminal kinase (JNK) and p38 signaling pathway. Importantly, dramatically reduced TIPE1 expression was observed in the livers of nonalcoholic fatty liver disease (NAFLD) patients, suggesting that TIPE1 might be a promising therapeutic target for NAFLD and related metabolic diseases. Conclusion: TIPE1 protects against hepatic steatosis, inflammation, and fibrosis through directly binding ASK1 and restraining its TRAF6-catalyzed polyubiquitination during the development of NASH. Therefore, targeting TIPE1 could be a promising therapeutic approach for NAFLD treatment.
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http://dx.doi.org/10.1002/hep.31801DOI Listing
March 2021

C-Kit, a Double-Edged Sword in Liver Regeneration and Diseases.

Front Genet 2021 2;12:598855. Epub 2021 Feb 2.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Previous studies have reported an important role of c-kit in embryogenesis and adulthood. Activation of the SCF/KIT signal transduction pathway is customarily linked to cell proliferation, migration and survival thus influence hematopoiesis, pigmentation, and spermatogenesis. The role of c-kit in the liver is controversial, it is however argued that it is a double-edged sword in liver regeneration and diseases. First, liver c-kit cells, including oval cells, bile epithelial cells, and part of hepatocytes, participate in liver tissue repair by regenerating target cells according to the type of liver injury. At the same time, c-kit mast cells, act as immature progenitors in circulation, playing a critical role in liver fibrosis. Furthermore, c-kit is also a proto-oncogene. Notably, c-kit overexpression regulates gastrointestinal stromal tumors. Various studies have explored on c-kit and hepatocellular carcinoma, nevertheless, the intricate roles of c-kit in the liver are largely understudied. Herein, we extensively summarize previous studies geared toward providing hints for future clinical and basic research.
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http://dx.doi.org/10.3389/fgene.2021.598855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884772PMC
February 2021

Long Non-coding RNA FOXD2-AS1 Promotes Proliferation, Migration, and Invasion in Cholangiocarcinoma Through Regulating miR-760/E2F3 Axis.

Dig Dis Sci 2021 Feb 11. Epub 2021 Feb 11.

Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, 246 Xuefu-ro, Harbin, 150086, People's Republic of China.

Background: Long non-coding RNA (lncRNA) has been testified to influence the initiation and evolution of sundry carcinomas. Recently, lncRNA FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1) has been found to display vital regulating functions in various cancers.

Methods: qRT-PCR was used to verify the dysregulation of FOXD2-AS1 expression in CCA cells and tissues, and the correlation of FOXD2-AS1 expression with clinicopathological characteristics was investigated. The viability, migration, and invasion of CCA cells were verified through CCK-8 assay, colony formation experiment, wound healing assay, and transwell assay. The regulatory networks of FOXD2-AS1 were analyzed by Bioinformatic prediction and dual-luciferase reporter assay.

Results: We discovered that FOXD2-AS1 was significantly upregulated in CCA and its up-regulation was closely correlated with terminal TNM stage, lymph node metastasis and poor survival in the current research. In addition, it was revealed that FOXD2-AS1 was an independent prognostic factor. Functional tests uncovered that the cell viability, migration, and invasion could be restrained through downregulating the expression of FOXD2-AS1, while FOXD2-AS1 overexpression could facilitate the cell viability, migration, and invasion. Mechanistically, FOXD2-AS1 was founded to interact directly with miR-760 and the oncogene E2F3 was the downstream target of miR-760 through bioinformatic prediction and dual-luciferase reporter assays. Finally, we testified that FOXD2-AS1 could competitively sponge miR-760 and further upregulated the E2F3 expression to play a vital part in cholangiocarcinoma.

Conclusions: This research revealed that lncRNA FOXD2-AS1 could enhance CCA malignant progression through regulating the miR-760/E2F3 axis and was expected to be a prognostic biomarker and therapeutic target for cholangiocarcinoma.
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http://dx.doi.org/10.1007/s10620-021-06876-9DOI Listing
February 2021

PCAT1 induced by transcription factor YY1 promotes cholangiocarcinoma proliferation, migration and invasion by sponging miR-216a-3p to up-regulate oncogene BCL3.

Biol Chem 2021 01 23;402(2):207-219. Epub 2020 Oct 23.

Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, No. 148 BaoJian-ro, Harbin150086, Heilongjiang, China.

This study was designed to illustrate the function and role of PCAT1 in CCA. The relative expression was confirmed by RT-qPCR and western blot. The biological function of PCAT1 was evaluated by CCK8, EdU, colony formation, wound healing, transwell, and subcutaneous tumor formation assays. Protein levels of EMT markers were measured by western blot. The binding relationship was predicted by JASPAR and starBase. The binding of YY1 to PCAT1 promoter was assessed by ChIP and luciferase reporter. The binding capacity between miR-216a-3p and PCAT1 as well as BCL3 was assessed by luciferase reporter and AGO2-RIP assays. In this study, we found that PCAT1 was up-regulated in CCA tissues and cells, and the PCAT1 overexpression was associated with poor prognosis. Moreover, PCAT1 was assessed as an independent risk factor of prognosis for CCA patients. Amplified PCAT1 was found to promote tumor proliferation, migration, invasion and EMT process, whereas PCAT1 knockdown inhibited these malignant phenotypes. Mechanistically, PCAT1 was predominantly localized in the cytoplasm and competitively bound miR-216a-3p to increase BCL3 expression. In addition, PCAT1 was activated by transcription factor YY1. This study revealed that PCAT1 acted as an oncogene in CCA, and the YY1/PCAT1/miR-216a-3p/BCL3 axis exhibited critical functions in CCA progression.
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http://dx.doi.org/10.1515/hsz-2020-0276DOI Listing
January 2021

More Symmetrical "Hot Spots" Ensure Stronger Plasmon-Enhanced Fluorescence: From Au Nanorods to Nanostars.

Anal Chem 2021 02 7;93(4):2480-2489. Epub 2021 Jan 7.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.

Plasmon-enhanced fluorescence (PEF) is considered to be a powerful signal amplification technology to overcome intrinsic shortcomings of photobleaching and brightness of the traditional fluorescent dyes. Nevertheless, exploitation of PEF-based probes for bioimaging application is still at a very early stage. In this work, a simple but powerful gold nanostar (Au NST)@SiO-based PEF probe with 20 symmetric "hot spots" was developed for highly sensitive "lighting up" in situ imaging of intracellular microRNAs (miRNAs). By regulating the thickness of the silica shell, the distance between Au NSTs and fluorescent dyes was controlled, and the optimum fluorescence enhancement (21-fold) was obtained with the silica shell thickness of approximately 22 nm. Thanks to the 20 more powerful "hot spots" that can produce stronger localized electric fields, the Au NST-based PEF probe exhibits stronger PEF effects than the traditional plasmonic nanostructures such as gold nanorods (Au NRs), gold nanobipyramids (Au NBPs), and triangular gold nanoprisms (Au NPRs), resulting in high sensitivity and improved detection limit (LOD) of 0.21 pM for miRNA-21 analysis. Moreover, not only cancer cells (MCF-7 and Hela) and normal cells (L02) with distinct miRNA-21 expression levels can be discriminated but also tumor cells in co-cultured mixtures can be recognized, indicating its promising potential in clinical diagnosis.
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http://dx.doi.org/10.1021/acs.analchem.0c04518DOI Listing
February 2021

Automated machine learning to predict the co-occurrence of isocitrate dehydrogenase mutations and O -methylguanine-DNA methyltransferase promoter methylation in patients with gliomas.

J Magn Reson Imaging 2021 07 3;54(1):197-205. Epub 2021 Jan 3.

Huaxi MR Research Center (HMRRC), Functional and Molecular Imaging Key Laboratory of Sichuan Province, Department of Radiology, West China Hospital of Sichuan University, Chengdu, China.

Combining isocitrate dehydrogenase mutation (IDHmut) with O -methylguanine-DNA methyltransferase promoter methylation (MGMTmet) has been identified as a critical prognostic molecular marker for gliomas. The aim of this study was to determine the ability of glioma radiomics features from magnetic resonance imaging (MRI) to predict the co-occurrence of IDHmut and MGMTmet by applying the tree-based pipeline optimization tool (TPOT), an automated machine learning (autoML) approach. This was a retrospective study, in which 162 patients with gliomas were evaluated, including 58 patients with co-occurrence of IDHmut and MGMTmet and 104 patients with other status comprising: IDH wildtype and MGMT unmethylated (n = 67), IDH wildtype and MGMTmet (n = 36), and IDHmut and MGMT unmethylated (n = 1). Three-dimensional (3D) T1-weighted images, gadolinium-enhanced 3D T1-weighted images (Gd-3DT1WI), T2-weighted images, and fluid-attenuated inversion recovery (FLAIR) images acquired at 3.0 T were used. Radiomics features were extracted from FLAIR and Gd-3DT1WI images. The TPOT was employed to generate the best machine learning pipeline, which contains both feature selector and classifier, based on input feature sets. A 4-fold cross-validation was used to evaluate the performance of automatically generated models. For each iteration, the training set included 121 subjects, while the test set included 41 subjects. Student's t-test or a chi-square test was applied on different clinical characteristics between two groups. Sensitivity, specificity, accuracy, kappa score, and AUC were used to evaluate the performance of TPOT-generated models. Finally, we compared the above metrics of TPOT-generated models to identify the best-performing model. Patients' ages and grades between two groups were significantly different (p = 0.002 and p = 0.000, respectively). The 4-fold cross-validation showed that gradient boosting classifier trained on shape and textual features from the Laplacian-of-Gaussian-filtered Gd-3DT1 achieved the best performance (average sensitivity = 81.1%, average specificity = 94%, average accuracy = 89.4%, average kappa score = 0.76, average AUC = 0.951). Using autoML based on radiomics features from MRI, a high discriminatory accuracy was achieved for predicting co-occurrence of IDHmut and MGMTmet in gliomas. LEVEL OF EVIDENCE: 3 TECHNICAL EFFICACY STAGE: 3.
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http://dx.doi.org/10.1002/jmri.27498DOI Listing
July 2021

Isoquercitrin induces apoptosis and autophagy in hepatocellular carcinoma cells via AMPK/mTOR/p70S6K signaling pathway.

Aging (Albany NY) 2020 11 29;12(23):24318-24332. Epub 2020 Nov 29.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.

Hepatocellular carcinoma (HCC) is an aggressive malignancy with high rates of metastasis and relapse. Isoquercitrin (ISO), a natural flavonoid present in the Chinese bayberry and other plant species, reportedly exerts notable inhibitory effects on tumor cell proliferation, though the mechanism is unknown. In the present study, we exposed HepG2 and Huh7 human liver cancer cells to ISO and examined the roles of autophagy and apoptosis in ISO-mediated cell death. We found that ISO exposure inhibited cell viability and colony growth, activated apoptotic pathway, and triggered dysregulated autophagy by activating the AMPK/mTOR/p70S6K pathway. Autophagy inhibition using 3-methyladenine (3-MA) or Atg5-targeted siRNA decreased the Bax/Bcl-2 ratio, caspase-3 activation, and PARP cleavage and protected cells against ISO-induced apoptosis. Moreover, autophagy inhibition reversed the upregulation of AMPK phosphorylation and downregulation of mTOR and p70S6K phosphorylation elicited by ISO. By contrast, application of a broad-spectrum caspase inhibitor failed to inhibit autophagy in ISO-treated cells. These data indicate that ISO simultaneously induced apoptosis and autophagy, and abnormal induction of autophagic flux contributed to ISO-triggered caspase-3-dependent apoptosis.
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http://dx.doi.org/10.18632/aging.202237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762471PMC
November 2020

A new species of the genus Grube, 1850 (Annelida, Ampharetidae) from the Yellow Sea, China, together with a redescription of Hutchings & Rainer, 1979.

Zookeys 2020 6;988:1-15. Epub 2020 Nov 6.

Laboratório de Poliquetologia, Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, travessa 14, n. 101, São Paulo, 05508-090, Brazil Macquarie University Sydney Australia.

A new species of the ampharetid genus , , is described based on material from the Yellow Sea. The new species is characterized by the possession of long, stout, golden paleae with blunt tips, digitiform rudimentary notopodia on the abdominal uncinigers, uncini with a subrostral process, and a narrow rectangular hump separating branchial groups. was redescribed from type materials at the Australian Museum, Sydney, and the differences between and are discussed. A key to distinguish species described or reported in Western Pacific waters is provided.
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http://dx.doi.org/10.3897/zookeys.988.49934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666089PMC
November 2020

α2A-Adrenergic Receptor Inhibits the Progression of Cervical Cancer Through Blocking PI3K/AKT/mTOR Pathway.

Onco Targets Ther 2020 15;13:10535-10546. Epub 2020 Oct 15.

Department of Gynecology and Obstetrics, The People's Liberation Army Navy, The 971th Hospital, Qingdao, Shandong 266071, People's Republic of China.

Objective: The study aimed to investigate the effect of α2A-adrenergic receptor (ADRA2A) on cervical cancer and the potential mechanisms of ADRA2A on phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway in cervical cancer cells.

Methods: In our study, ADRA2A expression was evaluated by analyzing cervical cancer RNA sequencing dataset from the GEPIA. The prognostic values of ADRA2A were evaluated by Kaplan-Meier method using the Cancer Genome Atlas (TCGA) database data. In addition, the expression of ADRA2A in cervical cancer cell lines was detected by qRT-PCR and Western blot. Subsequently, the roles of ADRA2A on cell proliferation, apoptosis, migration, invasion and senescence in HeLa and SiHa cells were evaluated. Moreover, tumorigenesis in nude mice was used to investigate the role of ADRA2A in vivo. We also detected the expression changes of key factors in PI3K/Akt/mTOR pathway after overexpression and silencing of ADRA2A in HeLa and SiHa cells.

Results: ADRA2A expression was significantly downregulated in cervical cancer tissues and cell lines. The high expression of ADRA2A was significantly associated with a better prognosis in cervical cancer patients. ADRA2A overexpression significantly suppressed cell proliferation, migration and invasion, and promoted cell senescence and apoptosis in cervical cancer cells. On the contrary, silencing ADRA2A dramatically facilitated cell proliferation, migration and invasion, and inhibited cell senescence and apoptosis in cervical cancer cells. The expressions of p-PI3K, p-AKT and p-mTOR in cervical cancer cells were notably decreased by ADRA2A overexpression and increased by silencing ADRA2A. In addition, we also confirmed that ADRA2A overexpression could suppress the xenograft tumor growth in vivo.

Conclusion: Our study demonstrated that ADRA2A could suppress cell proliferation, migration and invasion, as well as promote cell senescence and apoptosis through inhibiting PI3K/Akt/mTOR pathway in cervical cancer.
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http://dx.doi.org/10.2147/OTT.S264409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574911PMC
October 2020

Long noncoding RNA HOTAIRM1 in human cancers.

Clin Chim Acta 2020 Dec 13;511:255-259. Epub 2020 Oct 13.

Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, No. 148 BaoJian Road, Harbin 150086, China. Electronic address:

Long noncoding RNAs (lncRNAs) are a group of RNAs over 200 nucleotides in length involved in diverse processes in tumor cells including proliferation, invasion and apoptosis. Given these facts, it is hardly accidental that variations in the expression of some lncRNAs have been found to be closely related to carcinogenesis and tumor growth and metastasis. HOTAIRM1, first discovered as an important factor for granulocytic differentiation in NB4 promyelocytic leukemia, has been shown to be a salient cancer-related lncRNA abnormally expressed in a variety of tumors. In this review, we summarize current evidence on the critical role of HOTAIRM1 in human malignancy, its potential mechanism of action and future use in the development of effective therapeutics.
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http://dx.doi.org/10.1016/j.cca.2020.10.011DOI Listing
December 2020

IFNA1 and IFNA13 Genes Confer Genetic Predisposition to Ankylosing Spondylitis-Associated Uveitis in a Chinese Population.

Curr Eye Res 2021 Apr 29;46(4):585-591. Epub 2020 Sep 29.

Department of Rheumatology, Shenzhen Hospital of Integrated Traditional and Western Medicine, Guangdong, China.

Objectives: Uveitis is considered the most frequent extra-articular manifestation of Ankylosing Spondylitis (AS). Genetic factors play an important role in the pathogenesis of AS with uveitis. In this study, we investigated susceptibility genes of AS concomitant with uveitis.

Methods: First, 9p21-24 was selected as the region of susceptibility genes. Second, several candidate genes were selected from this region by bioinformatics analysis. Thirdly, a two-stage case control study was used to verify these candidate genes. The first stage included 50 AS with uveitis, 50 AS without uveitis, and 145 healthy controls. The qPCR+direct sequencing and cloning sequencing were used to identify SNPs in this stage. The second stage included 245 AS with uveitis, 360 AS without uveitis, and 530 healthy controls.

Results: IFNA1, IFNB1, IFNA8, and IFNA13 were selected as candidate genes. In the first stage, it was found that the frequency of IFNA1 rs28383797 (G allele) and IFNA13 rs653778 (T allele) in AS with uveitis was higher than that in AS without uveitis and healthy controls. In the second stage, more samples were used to verify the two SNPs. After comprehensive data analysis in the two stages, the frequency of rs28383797 G allele and rs653778 T allele in AS with uveitis was significantly higher than that in AS without uveitis ( = 1.9 × 10, OR = 2.161, 95%CI = 1.609-2.902 and = 1.5 × 10, OR = 2.028, 95%CI = 1.610-2.554) and healthy controls.

Conclusions: Rs653778 (T allele) of IFNA13 and rs28383797 (G allele) of IFNA1 were associated with AS concomitant uveitis, and may act as the susceptibility genes of AS with uveitis in the Chinese population.
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http://dx.doi.org/10.1080/02713683.2020.1817490DOI Listing
April 2021

Tetrahydropalmatine protects against acute lung injury induced by limb ischemia/reperfusion through restoring PI3K/AKT/mTOR-mediated autophagy in rats.

Pulm Pharmacol Ther 2020 10 17;64:101947. Epub 2020 Sep 17.

Department of Anesthesiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. Electronic address:

Background: Limb ischemia/reperfusion (I/R) is a common clinical process that frequently induces acute lung injury (ALI). Tetrahydropalmatine (THP) is a major bioactive constituent of various traditional Chinese medicine with protective effects on inflammation and oxidation. In this study, we aimed to investigate the possible protective effect of THP on ALI induced by limb I/R.

Methods: Rats were used to establish ALI through limb I/R. After administration of three doses of THP, the lung injury was evaluated by hematoxylin-eosin staining, tissue wet/dry weight ratio and ELISA examination of myeloperoxidase (MPO), malondialdehyde (MDA) and Super Oxide Dismutase (SOD). Additionally, PI3K/AKT/mTOR pathway and autophagy markers were determined by Western blot. To confirm the role of autophagy in the effect of THP on ALI, 3-methyladenine (3-MA), THP or THP + rapamycin (RAPA) was given to the model rats, and then evaluated the parameters above mentioned.

Results: The pulmonary histological lesions and wet/dry were significantly induced after limb I/R. Concurrently, I/R significantly increased MPO and MDA, and decreased SOD in lung tissues. These changes were reversed after THP treatment. Additionally, THP exerted inhibitory effect on the I/R-induced decrease of phosphorylation of PI3K/AKT/mTOR and increase of autophagy activity. The effects by THP on lung injury, PI3K/AKT/mTOR signaling and autophagy were also observed after treatment with 3-MA, an autophagy inhibitor, whereas were blocked by combinational treatment with RAPA, an autophagy inducer.

Conclusion: Our data suggested that THP had significant protection against ALI and this might be achieved by autophagy inhibition through rescuing PI3K/AKT/mTOR activity.
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http://dx.doi.org/10.1016/j.pupt.2020.101947DOI Listing
October 2020

Association between Thiopurines Use and Pregnancy Outcomes in Female Patients with Inflammatory Bowel Disease: A Meta-Analysis.

Curr Pharm Des 2021 ;27(19):2317-2324

Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Conflicting data exist regarding the influence of thiopurines exposure on adverse pregnancy outcomes in female patients with inflammatory bowel disease (IBD).

Objective: The aim of this study was to provide an up-to-date and comprehensive assessment of the safety of thiopurines in pregnant IBD women.

Methods: All relevant articles reporting pregnancy outcomes in women with IBD received thiopurines during pregnancy were identified from the databases (PubMed, Embase, Cochrane Library, and ClinicalTrials.gov) with the publication data up to April 2020. Data of included studies were extracted to calculate the relative risk (RR) of multiple pregnancy outcomes: congenital malformations, low birth weight (LBW), preterm birth, small for gestational age (SGA), and spontaneous abortion. The meta-analysis was performed using the random-effects model.

Results: Eight studies matched with the inclusion criteria and a total of 1201 pregnant IBD women who used thiopurines and 4189 controls comprised of women with IBD received drugs other than thiopurines during pregnancy were included. Statistical analysis results demonstrated that the risk of preterm birth was significantly increased in the thiopurine-exposed group when compared to IBD controls (RR, 1.34; 95% CI, 1.00-1.79; p=0.049; I =41%), while no statistically significant difference was observed in the incidence of other adverse pregnancy outcomes.

Conclusion: Thiopurines used in women with IBD during pregnancy is not associated with congenital malformations, LBW, SGA, or spontaneous abortion, but appears to have an association with an increased risk of preterm birth.
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http://dx.doi.org/10.2174/1381612826666200916144249DOI Listing
January 2021

Knockdown of lncRNA Suppressed the Proliferation of Cholangiocarcinoma by Sponging .

Cancer Biother Radiopharm 2020 Sep 9. Epub 2020 Sep 9.

Department of General Surgery and The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.

A large number of studies had found that small nucleolar RNA host gene 20 () was a long noncoding RNA (lncRNA) that played important regulatory functions in numerous tumors. Nevertheless, the expression and pathophysiological role of in cholangiocarcinoma (CCA) are currently unclear. The objective of this study is to reveal the clinical significance and pathophysiological function of in CCA. The tumor tissues and adjacent normal tissues of CCA were obtained to determine the expression and clinical significance of , and the targets of related genes were predicted through bioinformatics analysis. The function and regulatory mechanism of in CCA were evaluated by transfection, CCK-8 experiment, and luciferase reporter assay. In CCA, was highly expressed. Overexpressed was markedly interrelated with the lymph node invasion and TNM stage. In addition, it could be used as indicator to evaluate the prognosis of patients. sponging could accelerate the proliferation of CCA tumor cells. acted as a tumor suppressor in CCA and could also serve as a prognostic indicator. Abolition of caused an antagonistic effect and diminished the impacts of knockdown. combined with could better predict the prognosis of CCA patients. These data confirmed the knockdown expression in CCA could inhibit the proliferation by means of sponging .
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http://dx.doi.org/10.1089/cbr.2020.4042DOI Listing
September 2020

Detection of Mycobacterium kansasii using a combination of loop-mediated isothermal amplification (LAMP) and lateral flow biosensors.

Int Microbiol 2021 Jan 2;24(1):75-82. Epub 2020 Sep 2.

Sichuan Center for Disease Control and Prevention, Chengdu, 610041, Sichuan Province, China.

Mycobacterium kansasii is an opportunistic pathogen that causes both intrapulmonary and extrapulmonary infections. The symptoms of the pulmonary diseases caused by M. kansasii closely resemble Mycobacterium tuberculosis. Rapid and accurate differentiation of M. kansasii from M. tuberculosis, as well as other mycobacteria, is crucial for developing effective therapeutics and disease treatment. In this study, we combined loop-mediated isothermal amplification (LAMP) with lateral flow biosensors (LFB) to detect M. kansasii, by targeting the species-specific sequence of rpoB, a gene which encodes the β subunit of bacterial RNA polymerase. The assay was validated to ensure that it was highly selective by testing M. kansasii, M. tuberculosis, other species of respiratory bacteria, and other nontuberculous mycobacteria. The detection limit of the assay was 1 fg/μL of DNA and 50 CFU of bacilli in sputum. The M. kansasii-LAMP-LFB assay is a fast, cheap, and accurate method for detecting M. kansasii by constant temperature amplification and simple interpretation.
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http://dx.doi.org/10.1007/s10123-020-00143-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872997PMC
January 2021

A Litopenaeus vannamei p70S6K gene is involved in the antioxidative and apoptosis under low temperature.

Fish Shellfish Immunol 2020 Nov 26;106:656-665. Epub 2020 Aug 26.

Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, 510631, PR China. Electronic address:

p70S6K is involved in cellular response, such as tumor metastases, the immune response and tissue repair in vertebrates. The role of p70S6K in these physiological processes in crustaceans remains, however, unknown. In this study, the Lvp70S6K was identified, containing a 5' UTR of 294 bp, an ORF of 1494 bp ad a 3' UTR of 211 bp, encoding 497 amino acids with a theoretical molecular weight of 70 kDa and an estimated isoelectric point of (pI) of 5.16. The multiple alignment found that Lvp70S6K was highly homologous with other invertebrates. Lvp70S6K mRNA was detected in all the tested tissues and the Lvp70S6K expression levels was significantly down-regulated and reached the lowest level (0.44-fold, p < 0.01) at 1.5 h after low temperature stress. The subcellular localization of Lvp70S6K could be detected in cytoplasm. ROS production was significantly up-regulation (1.19-fold, p < 0.01), total hemocyte count (THC) was significantly down-regulation (0.22-fold, p < 0.01), apoptosis rate was markedly increased (1.09-fold, p < 0.01), apoptosis-related genes of LvPDCD4 (1.61-fold, p < 0.01) and LvCyt.C (1.23-fold, p < 0.01) were up-regulated, and anti-apoptotic gene of LvBcl-2 (0.69-fold, p < 0.01), LvIAP1 (0.68-fold, p < 0.01) and LvIAP2 (0.45-fold, p < 0.01) were decreased after low temperature stress in hemolymph of Lvp70S6K-silenced shrimp at 1.5 h. Silencing of LvPTEN significantly increased Lvp70S6K, LvPI3K, LvAKT and LvmTOR expression. In summary, these results indicated that Lvp70S6K play a crucial role in oxidative and apoptosis, which was able to negatively regulate by PTEN.
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http://dx.doi.org/10.1016/j.fsi.2020.08.010DOI Listing
November 2020

LncRNA PCAT6 promotes the proliferation, migration and invasion of pancreatic ductal adenocarcinoma via regulating miR-185-5p/CBX2 axis.

Pathol Res Pract 2020 Sep 18;216(9):153074. Epub 2020 Jun 18.

Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086, China. Electronic address:

Emerging evidence has revealed markedly roles for long noncoding RNAs (lncRNAs) in various cancer processes. Prostate cancer associated transcript 6 (PCAT6) is a novel lncRNA which displays vital regulatory functions in multiple cancers. However, the functions of PCAT6 in pancreatic ductal adenocarcinoma (PDAC) remain unclear. Our study confirmed that PCAT6 expression was upregulated in PDAC and the expression of PCAT6 was related to TNM stage, lymph node invasion and overall survival of PDAC patients. PCAT6 might act as an effective tumor biomarker for PDAC patients. Moreover, knockdown of PCAT6 inhibited cell proliferation, migration and invasion of PDAC in vitro. For the mechanism, miR-185-5p expression was decreased and chromobox 2 (CBX2) expression was increased in PDAC, and further PCAT6 could upregulated the expression of oncogene CBX2 by sponging miR-185-5p. The results above suggested that PCAT6/miR-185-5p/CBX2 exerted crucial functions in tumorigenesis and progression of PDAC.
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http://dx.doi.org/10.1016/j.prp.2020.153074DOI Listing
September 2020

Rheumatoid arthritis-associated interstitial lung disease: an overview of epidemiology, pathogenesis and management.

Clin Rheumatol 2021 Apr 13;40(4):1211-1220. Epub 2020 Aug 13.

Department of Rheumatology and Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 JieFang Road, Wuhan, HuBei, China.

Interstitial lung disease (ILD) accounts for the major cause of morbidity and mortality in rheumatoid arthritis (RA). However, little is known of the pathogenesis, diagnosis and treatment of RA-associated ILD. In this review, we describe our present understanding and ongoing research in RA-ILD. Its aetiology does appear to associate with anti-cyclic citrullinated peptide antibodies, MUC5B mutation and smoking. Another focus of this article is on recent diagnostic methods in RA-ILD. Compared with other methods, high-resolution computed tomography (HRCT) imaging is a main method for the evaluation of ILD in RA patients. Pulmonary function is better suited for assessing progression. An important topic relates to therapeutic intervention. Disease-modifying anti-rheumatic drugs (DMARDs) in RA lack strong evidence in the onset or worsening of ILD. The available literature support that methotrexate, leflunomide, abatacept and rituximab play beneficial roles in the prevention and treatment of RA-ILD.
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http://dx.doi.org/10.1007/s10067-020-05320-zDOI Listing
April 2021

Review of the genus Malmgren, 1866 (Annelida, Terebellidae) from China seas with recognition of two new species based on integrative taxonomy.

PeerJ 2020 16;8:e9491. Epub 2020 Jul 16.

Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Specimens of the genus (Annelida, Terebellidae) deposited in the Marine Biological Museum of the Chinese Academy of Sciences (MBMCAS) together with materials newly collected from China seas were examined. Based on morphological comparisons and molecular analysis, some specimens collected from the coasts of Shandong province and Guangxi province were confirmed as two new species respectively ( sp. n. and sp. n.). Morphologically, sp. n. is distinguished from previously known species of this genus by having seven equal sized ventral shields, with length five times the width; this species was retrieved as sister to the clades of Moore, 1903 and Hutchings, 1990 in the phylogenetic tree, which was reconstructed based on mitochondrial COI gene. sp. n. differs from congeners by the large size of its first pair of branchiae with a thick main stem and about 18 dendritic branches arranged in two levels. A key to identifying species found in Chinese seas is given.
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http://dx.doi.org/10.7717/peerj.9491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369024PMC
July 2020

The microRNA/TET3/REST axis is required for olfactory globose basal cell proliferation and male behavior.

EMBO Rep 2020 09 17;21(9):e49431. Epub 2020 Jul 17.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.

In the main olfactory epithelium (MOE), new olfactory sensory neurons (OSNs) are persistently generated to replace lost neurons throughout an organism's lifespan. This process predominantly depends on the proliferation of globose basal cells (GBCs), the actively dividing stem cells in the MOE. Here, by using CRISPR/Cas9 and RNAi coupled with adeno-associated virus (AAV) nose delivery approaches, we demonstrated that knockdown of miR-200b/a in the MOE resulted in supernumerary Mash1-marked GBCs and decreased numbers of differentiated OSNs, accompanied by abrogation of male behaviors. We further showed that in the MOE, miR-200b/a targets the ten-eleven translocation methylcytosine dioxygenase TET3, which cooperates with RE1-silencing transcription factor (REST) to exert their functions. Deficiencies including proliferation, differentiation, and behaviors illustrated in miR-200b/a knockdown mice were rescued by suppressing either TET3 or REST. Our work describes a mechanism of coordination of GBC proliferation and differentiation in the MOE and olfactory male behaviors through miR-200/TET3/REST signaling.
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http://dx.doi.org/10.15252/embr.201949431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507367PMC
September 2020

Clinicians versus patients subjective adverse events assessment: based on patient-reported outcomes version of the common terminology criteria for adverse events (PRO-CTCAE).

Qual Life Res 2020 Nov 20;29(11):3009-3015. Epub 2020 Jun 20.

Breast Center of the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.

Purpose: Adverse events (AEs) assessment by clinicians is a standard practice in a clinical setting. However, studies have found clinicians tend to report fewer AEs, especially subjective AEs. We aimed to explore the difference of subjective AEs assessment between clinicians and patients based on PRO-CTCAE, and to discuss the necessity of incorporating patient into the evaluation of AEs.

Methods: Between April and July 2019, two different questionnaires with the same subjective AEs were given to patients and clinicians in the Day Chemotherapy ward of Breast Center in the Fourth Hospital of HeBei Medical University. Patients completed a Simplified Chinese version of PRO-CTCAE, including six common subjective AEs of chemotherapy: nausea, vomiting, diarrhea, fatigue, pain, and constipation. Clinicians completed the common terminology criteria for adverse events (CTCAE) with the same AEs. General information of enrolled patients and results from the questionnaires were collected and analyzed.

Results: 384 paired questionnaires were collected. Clinicians reported less subjective AEs than patients, and the general agreement between patients and clinicians was poor. When considering the grade difference, we utilize weighted kappa coefficient to analysis, and agreement between patients and clinicians was poor (k < 0.4) regardless of the frequency, the severity and interfering with daily life of AEs, and the most discrepancies were within one point. Patients tended to grade severer than the clinician.

Conclusions: The results of this study showed that there were differences between clinicians and patients in subjective adverse events evaluation. Patient reporting of symptoms can be used as a supplementary method to incorporate the current approach to monitor subjective AEs, to improve the timeliness and accuracy of clinical evaluation of subjective AEs.
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http://dx.doi.org/10.1007/s11136-020-02558-7DOI Listing
November 2020

Atmospheric Chemistry of Enols: The Formation Mechanisms of Formic and Peroxyformic Acids in Ozonolysis of Vinyl Alcohol.

J Phys Chem A 2020 May 18;124(21):4271-4279. Epub 2020 May 18.

Key Laboratory for Macromolecular Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, Shaanxi, China.

Vinyl alcohol (VA), for a long time, is thought to be a missing source of formic acid (FA) in the atmospheric models. However, a recent study has shown that FA is just a byproduct in the OH-initiated oxidation of VA, which stimulates investigation on the other sinks of VA in the atmosphere. In this study, the detailed ozonolysis mechanism of VA was investigated theoretically for the first time. The results show that two primary ozonides (- and -POZ) can be formed in the ozonolysis of VA and that FA coupled with the simplest Criegee intermediate (CHOO) can be produced as the main nascent products. Thus, the ozonolysis of VA is predicted to be a more efficient process to produce FA in the atmosphere compared with its OH-initiated oxidation. Moreover, it is found that the -POZ can directly decompose to peroxyformic acid plus formaldehyde, breaking the known "Criegee mechanism" to form carbonyl oxide with carbonyl compound. This special mechanism by providing a new source of peroxy acids in the atmosphere enriches the atmospheric chemistry of enols. The atmospheric lifetime of VA by ozonolysis is predicted to be 30 h, comparable with its prevalent reaction with the OH radical. Therefore, the obtained theoretical results can be usefully incorporated into a future modeling study of enols.
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http://dx.doi.org/10.1021/acs.jpca.0c01480DOI Listing
May 2020
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