Publications by authors named "Weimin Chen"

232 Publications

Clinical characteristics of optic neuritis phenotypes in a 3-year follow-up Chinese cohort.

Sci Rep 2021 Jul 16;11(1):14603. Epub 2021 Jul 16.

Department of Ophthalmology, Eye Ear Nose and Throat Hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, China.

To evaluate the clinical characteristics of optic neuritis (ON) with different phenotypes. This prospective study recruited patients with new-onset ON between January 2015 and March 2017 who were followed-up for 3 years. They were divided into the myelin oligodendrocyte glycoprotein-seropositive (MOG-ON), aquaporin-4-seropositive (AQP4-ON), and double-seronegative (seronegative-ON) groups, and their clinical characteristics and imaging findings were evaluated and compared. Two-hundred-eighty patients (405 eyes) were included (MOG-ON: n = 57, 20.4%; AQP4-ON: n = 98, 35.0%; seronegative-ON: n = 125, 44.6%). The proportion of eyes with best-corrected visual acuity > 20/25 at the 3-year follow-up was similar between the MOG-ON and seronegative-ON groups; the proportion in both groups was higher than that in the AQP4-ON group (p < 0.001). Relapse rates were higher in the MOG-ON and AQP4-ON groups than in the seronegative-ON group (p < 0.001). Average retinal nerve fiber layer (RNFL) thickness at 3 years was similar between the MOG-ON and AQP4-ON groups (63.41 ± 13.39 and 59.40 ± 11.46 μm, p = 0.476) but both were thinner than the seronegative-ON group (74.06 ± 11.14 μm, p < 0.001). Macular ganglion cell-inner plexiform layer (GCIPL) revealed the same pattern. Despite RNFL and GCIPL thinning, the MOG-ON group's outcome was as favorable as that of the seronegative-ON group, whereas the AQP4-ON group showed unsatisfactory results.
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http://dx.doi.org/10.1038/s41598-021-93976-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285465PMC
July 2021

A single homogeneous assay for simultaneous measurement of bispecific antibody target binding.

J Immunol Methods 2021 Jul 2;496:113099. Epub 2021 Jul 2.

Department of Analytical Sciences, BioPharmaceuticals Development, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA.

Bispecific antibodies (BsAbs) are engineered to simultaneously bind two different antigens, and offer promising clinical outcomes for various diseases. The dual binding properties of BsAbs may enable superior efficacies and/or potencies compared to standard monoclonal antibodies (mAbs) or combination mAb therapies. Characterizing BsAb binding properties is critical during biotherapeutic development, where data is leveraged to predict efficacy and potency, assess critical quality attributes and improve antibody design. Traditional single-target, single-readout approaches (e.g., ELISA) have limited usefulness for interpreting complex bispecific binding, and double the benchwork. To address these deficiencies, we developed and implemented a new dual-target/readout binding assay that accurately dissects the affinities of both BsAb binding domains directly and simultaneously. This new assay uses AlphaPlex® technology, which eliminates traditional ELISA wash steps and can be miniaturized for automated workflows. The optimized BsAb AlphaPlex assay demonstrates 99-107% accuracy within a 50-150% linear range, and detected >50% binding degradation from photo- and thermal stress conditions. To the best of our knowledge, this is the first instance of a dual-target/readout BsAb AlphaPlex assay with GMP-suitable linear range, accuracy, specificity, and stability-indicating properties. As a highly customizable and efficient assay, BsAb AlphaPlex may be applicable to numerous bispecific formats and/or co-formulations against a variety of antigens beyond the clinical therapeutic space.
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http://dx.doi.org/10.1016/j.jim.2021.113099DOI Listing
July 2021

Stochastic community assembly decreases soil fungal richness in arid ecosystems.

Mol Ecol 2021 Jun 29. Epub 2021 Jun 29.

State Key Laboratory of Crop Stress Biology in Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, China.

Uncovering the linkages between community assembly and species diversity is a fundamental issue in microbial ecology. In this study, a large-scale (transect intervals of 1257.6 km) cross-biome soil survey was conducted, which ranged over agricultural fields, forests, wetlands, grasslands and desert, in the arid regions of northwest China. The aim was to investigate the biogeographic distribution, community assembly and species co-occurrence of soil fungi. The fungal communities in agricultural soils exhibited a steeper distance-decay slope and wider niche breadths, and were more strongly affected by stochastic assembly processes, than fungi in other natural habitats. A strong relationship was revealed between soil fungal richness and community assembly in arid ecosystems, with the influence of stochastic assembly processes decreasing with increasing fungal richness. Moreover, aridity was the most important environmental factor influencing fungal richness, β-diversity and species co-occurrence patterns. Specifically, the predicted increase in arid conditions will probably reduce fungal richness and network complexity. These findings represent a considerable advance in linking fungal richness to mechanisms underlying the biogeographic patterns and assembly processes of fungal communities in arid ecosystems. These results can thus be used to forecast species co-occurrence and diversities pattern of soil fungi under climate aridity and land-use change scenarios.
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http://dx.doi.org/10.1111/mec.16047DOI Listing
June 2021

Mechanism of bone marrow mesenchymal stem cells secreting miR-26a exosomes affecting high glucose-induced skin fibroblasts function by regulating TLR4/NF-κB signaling.

Inflamm Res 2021 Jul 29;70(7):811-821. Epub 2021 Jun 29.

Division of Periodontology, Diagnostic Sciences, and Dental Hygiene, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.

Objective: The aim of this study was to investigate the molecular mechanism of human bone marrow mesenchymal stem cells (hMSCs) secreting miR-26a exosomes on the function of skin fibroblasts.

Methods: Exosomes from hMSCs were extracted and identified by transmission electron microscopy, particle size was analyzed and protein markers were detected. Then, the exosomes were co-cultured with human skin fibroblasts (BJ). CCK-8, Annexin V/P and Transwell assays were used to detect the proliferation, apoptosis, and migration of BJ cells. In addition, the expressions of miR-26a, related proteins, and related inflammatory factors were detected by qRT-PCR, western blotting, and ELISA.

Results: Compared with the high glucose group, the proliferation rate, migration rate, and the expression of α-SMA, bcl-2, TLR4, NF-κB, TNF-α, IL-6, IL- and IL-1 were significantly decreased in the high glucose + MSC-Exo-miR-26a mimics group, while the apoptosis rate and the expression of miR-26a, cleaved-caspase 3, cleaved-caspase 9 and Bax were significantly increased. The results of the high glucose + MSC-Exo-miR-26a inhibitor group were the opposite.

Conclusion: These results suggest that hMSCs cells secreting miR-26a exosomes inhibited the proliferation, migration, and transdifferentiation of high glucose-induced BJ cells, and promoted cell apoptosis, which may be related to the TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.1007/s00011-021-01478-7DOI Listing
July 2021

Identification of autophagy-related gene and lncRNA signatures in the prognosis of HNSCC.

Oral Dis 2021 Apr 26. Epub 2021 Apr 26.

Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: The aim of this study was to identify prognostic autophagy-related genes and lncRNAs to predict clinical outcomes in head and neck squamous cell carcinoma (HNSCC).

Subjects And Methods: Differentially expressed autophagy-related genes and autophagy-related lncRNAs were identified by comparing pare-carcinoma and carcinoma samples of HNSCC. And then, we constructed an ARG and an AR-lncRNA signature risk score. Receiver operating characteristic (ROC) curve analyses were performed to assess the prognostic prediction capacity. Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO) functional annotation were used to analysis the functions of ARGs and AR-lncRNAs.

Results: Six ARGs and thirteen AR-lncRNAs were identified in the ARG and AR-lncRNA signatures, and overall survival (OS) in the high-risk group was significantly shorter than the low-risk group. ROC analysis showed the ARG and AR-lncRNA signatures have excellent ability of predicting the total OS of patients with HNSCC. What's more, GSEA and GO functional annotation proved that autophagy-related pathways are mainly enriched in the high-risk group.

Conclusions: These findings indicated that our ARG signature and AR-lncRNA signature could be considered to predict the prognosis of patients with HNSCC and provide a deep understanding of the biological mechanisms of autophagy in HNSCC.
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http://dx.doi.org/10.1111/odi.13889DOI Listing
April 2021

A high-conductivity n-type polymeric ink for printed electronics.

Nat Commun 2021 Apr 21;12(1):2354. Epub 2021 Apr 21.

Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, Norrköping, Sweden.

Conducting polymers, such as the p-doped poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), have enabled the development of an array of opto- and bio-electronics devices. However, to make these technologies truly pervasive, stable and easily processable, n-doped conducting polymers are also needed. Despite major efforts, no n-type equivalents to the benchmark PEDOT:PSS exist to date. Here, we report on the development of poly(benzimidazobenzophenanthroline):poly(ethyleneimine) (BBL:PEI) as an ethanol-based n-type conductive ink. BBL:PEI thin films yield an n-type electrical conductivity reaching 8 S cm, along with excellent thermal, ambient, and solvent stability. This printable n-type mixed ion-electron conductor has several technological implications for realizing high-performance organic electronic devices, as demonstrated for organic thermoelectric generators with record high power output and n-type organic electrochemical transistors with a unique depletion mode of operation. BBL:PEI inks hold promise for the development of next-generation bioelectronics and wearable devices, in particular targeting novel functionality, efficiency, and power performance.
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http://dx.doi.org/10.1038/s41467-021-22528-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060302PMC
April 2021

An Efficient Deep-Subwavelength Second Harmonic Nanoantenna Based on Surface Plasmon-Coupled Dilute Nitride GaNP Nanowires.

Nano Lett 2021 Apr 19;21(8):3426-3434. Epub 2021 Apr 19.

Key Laboratory for Micro-Nano Physics and Technology of Hunan Province, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Materials Science and Engineering, Hunan University, Changsha, Hunan 410082, People's Republic of China.

High-index semiconductor nanoantennae represent a powerful platform for nonlinear photon generation. Devices with reduced footprints are pivotal for higher integration capacity and energy efficiency in photonic integrated circuitry (PIC). Here, we report on a deep subwavelength nonlinear antenna based on dilute nitride GaNP nanowires (NWs), whose second harmonic generation (SHG) shows a 5-fold increase by incorporating ∼0.45% of nitrogen (N), in comparison with GaP counterpart. Further integrating with a gold (Au) thin film-based hybrid cavity achieves a significantly boosted SHG output by a factor of ∼380, with a nonlinear conversion efficiency up to 9.4 × 10 W. In addition, high-density zinc blende (ZB) twin phases were found to tailor the nonlinear radiation profile via dipolar interference, resulting in a highly symmetric polarimetric pattern well-suited for coupling with polarization nano-optics. Our results manifest dilute nitride nanoantenna as promising building blocks for future chip-based nonlinear photonic technology.
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http://dx.doi.org/10.1021/acs.nanolett.0c05115DOI Listing
April 2021

Development of a mechanism of action-reflective, dual target cell-based reporter bioassay for a bispecific monoclonal antibody targeting human CTLA-4 and PD-1.

MAbs 2021 Jan-Dec;13(1):1914359

Bioassay Development, Biopharmaceutical Development, AstraZeneca, Gaithersburg, MD, USA.

T-cell-mediated immunotherapy has generated much excitement after the success of therapeutic biologics targeting immune checkpoint molecules. Bispecific antibodies (BsAbs) that recognize two antigen targets are a fast-growing class of biologics offering promising clinical benefits for cancer immunotherapy. Due to the complexity of the molecule structure and the potential mechanism of action (MOA) that involves more than one signaling pathway, it is critical to develop appropriate bioassays for measuring potency and characterizing the biological properties of BsAbs. Here, we present a dual target, cell-based reporter bioassay for a BsAb that binds human CTLA-4 and PD-1 and targets two subsequent signaling pathways that negatively regulate T-cell activation. This reporter bioassay is capable of measuring the potency of both antigen target arms in one assay, which would not be achievable using two single target bioassays. This dual target reporter bioassay demonstrates good performance characteristics suitable for lot release, stability testing, critical quality attribute assessment, and biological properties characterization of the CTLA-4/PD-1 BsAb. Furthermore, this assay can capture the synergistic effect of anti-CTLA-4 and anti-PD-1 activity of the BsAb. Compared to single target assays, this dual target bioassay could better reflect the potential MOA of BsAbs and could be used for evaluation of other bispecific biologics, as well as antibody combination therapies.
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http://dx.doi.org/10.1080/19420862.2021.1914359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078673PMC
April 2021

Circadian Rhythm Gene PER3 Negatively Regulates Stemness of Prostate Cancer Stem Cells via WNT/β-Catenin Signaling in Tumor Microenvironment.

Front Cell Dev Biol 2021 18;9:656981. Epub 2021 Mar 18.

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Prostate cancer (PCa) cells are heterogeneous, containing a variety of cancer cells with phenotypical and functional discrepancies in the tumor microenvironment, where prostate cancer stem cells (PCSCs) play a vital role in PCa development. Our earlier studies have shown that ALDHCD44 (DP) PCa cells and the corresponding ALDHCD44 (DN) PCa cells manifest as PCSCs and non-PCSCs, respectively, but the underlying mechanisms regulating stemness of the PCSCs are not completely understood. To tackle this issue, we have performed RNA-Sequencing and bioinformatic analysis in DP (versus DN) cells in this study. We discovered that, PER3 (period circadian regulator 3), a circadian rhythm gene, is significantly downregulated in DP cells. Overexpression of PER3 in DP cells significantly suppressed their sphere- and colony-forming abilities as well as tumorigenicity in immunodeficient hosts. In contrast, knockdown of PER3 in DN cells dramatically promoted their colony-forming and tumor-initiating capacities. Clinically, PER3 is downregulated in human prostate cancer specimens and PER3 expression levels are highly correlated with the prognosis of the PCa patient. Mechanistically, we observed that low levels of PER3 stimulates the expression of BMAL1, leading to the phosphorylation of β-catenin and the activation of the WNT/β-catenin pathway. Together, our results indicate that PER3 negatively regulates stemness of PCSCs via WNT/β-catenin signaling in the tumor microenvironment, providing a novel strategy to treat PCa patients.
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http://dx.doi.org/10.3389/fcell.2021.656981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012816PMC
March 2021

Detection of lung cancer with electronic nose using a novel ensemble learning framework.

J Breath Res 2021 Feb 12. Epub 2021 Feb 12.

Key Laboratory of Biotechnology Science and Technology, Ministry of Education, College of Bioengineering, , Chongqing University, No.174 Shazhengjie, Shapingba, Chongqing, Chongqing, 400044, CHINA.

Breath analysis based on electronic nose (e-nose) is a promising new technology for the detection of lung cancer that is non-invasive, simple to operate and cost-effective. Lung cancer screening by e-nose relies on predictive models established using machine learning methods. However, using only a single machine learning method to detect lung cancer has some disadvantages, including low detection accuracy and high false negative rate. To address these problems, groups of individual learning models with excellent performance were selected from classic models, including Support Vector Machine, Decision Tree, Random Forest, Logistic Regression and K-nearest neighbor regression, to build an ensemble learning framework (PCA-SVE). The output result of the PCA-SVE framework was obtained by voting. To test this approach, we analyzed 214 breath samples measured by e-nose with 11 gas sensors of four types using the proposed PCA-SVE framework. Experimental results indicated that the accuracy, sensitivity, and specificity of the proposed framework were 95.75%, 94.78%, and 96.96%, respectively. This framework overcomes the disadvantages of a single model, thereby providing an improved, practical alternative for exhaled breath analysis by e-nose.
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http://dx.doi.org/10.1088/1752-7163/abe5c9DOI Listing
February 2021

Expression and Role of Dickkopf-1 (Dkk1) in Tumors: From the Cells to the Patients.

Cancer Manag Res 2021 25;13:659-675. Epub 2021 Jan 25.

Guizhou Medical University, Guiyang, Guizhou Province 550002, People's Republic of China.

() is a secretory antagonist of the classical Wnt signaling pathway. Many studies have reported that is abnormally expressed in tumor cells, and abnormal expression of can inhibit cell proliferation or induce apoptosis through pro-apoptotic factors, However, due to the differences in tumor environment and the complex regulatory mechanisms in different tumors, has different effects on the progression of different tumors. In many tumors, high expression of may promote tumor metastasis. However, , which is highly expressed in other tumors, can inhibit tumor invasion and metastasis. More and more evidence shows that plays a complex and different role in tumor occurrence, development and metastasis in different tumor environments and through a variety of complex regulatory mechanisms. Therefore, may not only be a useful biomarker of metastasis, but also a target for studying the metabolic mechanism of tumor cells and treating tumors in many tumor types. Therefore, this article reviews the research progress on the expression, mechanism and function of in different tumors, and at the same time, based on the public database data, we made a further analysis of the expression of in different tumors.
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http://dx.doi.org/10.2147/CMAR.S275172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847771PMC
January 2021

Effect of Root Diameter on the Selection and Network Interactions of Root-Associated Bacterial Microbiomes in Robinia pseudoacacia L.

Microb Ecol 2021 Jan 15. Epub 2021 Jan 15.

Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, 3 Taicheng Road, Yangling, Xianyang, 712100, Shaanxi, People's Republic of China.

The high plasticity of root morphology, physiology, and function influences root-associated microbiomes. However, the variation in root-associated microbiome diversity and structures in response to root diameter at different root depths remains poorly understood. Here, we selected black locust (Robinia pseudoacacia L.) as a model plant to investigate the selection and network interactions of rhizospheric and root endophytic bacterial microbiomes associated with roots of different diameters (1, 1-2, and > 2 mm) among root depths of 0-100 cm via the Illumina sequencing of the 16S rRNA gene. The results showed that the alpha diversity of the root-associated bacterial communities decreased with increasing root diameters among different root depths; fewer orders with higher relative abundance, especially in the endosphere, were enriched in association with coarse roots (> 2 mm) than fine roots among root depths. Furthermore, the variation in the enriched bacterial orders associated with different root diameters was explained by bulk soil properties. Higher co-occurrence network complexity and stability emerged in the rhizosphere microbiomes of fine roots than those of coarse roots, in contrast to the situation in the endosphere microbiomes. In particular, the endosphere of roots with a diameter of 1-2 mm exhibited the lowest network complexity and stability and a high proportion of keystone taxa (e.g., Cytophagia, Flavobacteriia, Sphingobacteriia, β-Proteobacteria, and γ-Proteobacteria), suggesting a keystone taxon-reliant strategy in this transitional stage. In summary, this study indicated that root diameter at different root depths differentially affects rhizospheric and endophytic bacterial communities, which implies a close relationship between the bacterial microbiome, root function, and soil properties.
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http://dx.doi.org/10.1007/s00248-020-01678-4DOI Listing
January 2021

Activity of murine surrogate antibodies for durvalumab and tremelimumab lacking effector function and the ability to deplete regulatory T cells in mouse models of cancer.

MAbs 2021 Jan-Dec;13(1):1857100

Discovery Biosciences, Oncology R&D, AstraZeneca , Gaithersburg, MD, USA.

Preclinical studies of PD-L1 and CTLA-4 blockade have relied heavily on mouse syngeneic tumor models with intact immune systems, which facilitate dissection of immunosuppressive mechanisms in the tumor microenvironment. Commercially developed monoclonal antibodies (mAbs) targeting human PD-L1, PD-1, and CTLA-4 may not demonstrate cross-reactive binding to their mouse orthologs, and surrogate anti-mouse antibodies are often used in their place to inhibit these immune checkpoints. In each case, multiple choices exist for surrogate antibodies, which differ with respect to species of origin, affinity, and effector function. To develop relevant murine surrogate antibodies for the anti-human PD-L1 mAb durvalumab and the anti-human CTLA-4 mAb tremelimumab, rat/mouse chimeric or fully murine mAbs engineered for reduced effector function were developed and compared with durvalumab and tremelimumab. Characterization included determination of target affinity, in vivo effector function, pharmacokinetic profile, and anti-tumor efficacy in mouse syngeneic tumor models. Results showed that anti-PD-L1 and anti-CTLA-4 murine surrogates with pharmacologic properties similar to those of durvalumab and tremelimumab demonstrated anti-tumor activity in a subset of commonly used mouse syngeneic tumor models. This activity was not entirely dependent on antibody-dependent cellular cytotoxicity, antibody-dependent cellular phagocytosis effector function, or regulatory T-cell depletion, as antibodies engineered to lack these features showed activity in models historically sensitive to checkpoint inhibition, albeit at a significantly lower level than antibodies with intact effector function.
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http://dx.doi.org/10.1080/19420862.2020.1857100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831362PMC
January 2021

Multi-Parameter Measurement of Rotors Using the Doppler Effect of Frequency-Swept Interferometry.

Sensors (Basel) 2020 Dec 15;20(24). Epub 2020 Dec 15.

Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Chongqing University, Chongqing 400044, China.

The Doppler effect of frequency-swept interferometry (FSI) is often seen as an obstacle to the dynamic ranging accuracy. However, the potential of this obstacle is rarely noticed and used. In this paper, by combining the periodical characteristics of the rotational Doppler effect, an FSI-based multi-parameter measurement method for the rotor is proposed. Through the establishment of the rotational Doppler formula of FSI, it is found that the frequency, direct component, and amplitude of the dynamic distance given by FSI can be utilized to estimate the angular velocity, axial clearance, and tilt angle of the rotor, respectively. A rotor platform and a fiber-optic FSI system were constructed, and a series of experiments were carried out to verify the proposed method. The experimental results showed that the relative errors of the measured axial clearance, angular velocity, and tilt angle were less than 3.5%. This work provides a new perspective on the multi-parameter measurement of the rotor and makes it possible to directly perform multi-parameter measurement inside the space-confined rotating machinery as only a single small-size fiber-optic probe is needed.
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http://dx.doi.org/10.3390/s20247178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765328PMC
December 2020

The Efficacy of Ultrasound for Visualizing Radial Nerve Lesions with Coexistent Plate Fixation of Humeral Shaft Fractures.

Injury 2021 Mar 16;52(3):516-523. Epub 2020 Nov 16.

Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Department of Hand and Upper Extremity Surgery, Jing' an District Central Hospital, Shanghai, China; Limb Function Reconstruction Center, Jing' an District Central Hospital, Shanghai, China; National Clinical Research Center for Aging and Medicine, Fudan University, Shanghai, China; Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai, China; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China; State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China; Research unit of synergistic reconstruction of upper and lower limbs after brain injury, Chinese Academy of Medical Sciences, Shanghai, China.

Introduction: Ultrasound has been commonly employed for depicting the morphology of the lesions in patients with radial nerve neuropathy, including entrapment, tumor, trauma, and iatrogenic injury. However, few studies have evaluated the efficacy of ultrasound for visualizing radial nerve lesions with coexistent plate fixation of humeral shaft fractures. This study aimed to address this special clinical issue.

Methods: We retrospectively examined the efficacy of ultrasound for visualizing radial nerve lesions with coexistent plate fixation of humeral shaft fractures based on intraoperative findings in patients who were treated in our hospital from January 2007 to June 2019.

Results: Forty-six patients were included, and there was a 100% concordance between the ultrasound and intraoperative findings on radial nerve lesions. Ultrasonography revealed four types of lesions: radial nerve in continuity in thirty-one patients, neuroma in continuity in four patients, radial nerve stuck under the plate in three patients, and radial nerve transection in eight patients. The lesion radial nerve in continuity comprised two situations according to intraoperative electrodiagnostic test results, which could not be differentiated by ultrasonography, radial nerve in continuity treated with neurolysis in twenty-five patients and radial nerve in continuity treated with nerve graft in six patients.

Conclusion: Ultrasonography can accurately depict radial nerve lesions with coexistent plate fixation of humeral shaft fractures. It provides a basis for determining the extent of nerve damage in all patients except those with the lesion radial nerve in continuity, which is conducive to making treatment decisions as early as possible.
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http://dx.doi.org/10.1016/j.injury.2020.11.042DOI Listing
March 2021

Targeting CD47/TNFAIP8 by miR-155 overcomes drug resistance and inhibits tumor growth through induction of phagocytosis and apoptosis in multiple myeloma.

Haematologica 2020 12 1;105(12):2813-2823. Epub 2020 Dec 1.

University Health Network, University of Toronto, Toronto, Canada.

The mechanisms of drug resistance in multiple myeloma are poorly understood. Here we show that CD47, an integrin-associated receptor, is significantly upregulated in drug resistant myeloma cells in comparison with parental cells, and that high expression of CD47 detected by immunohistochemistry is associated with shorter progression free and overall survivals in multiple myeloma patients. We show that miR-155 is expressed at low levels in drug resistant myeloma cells and is a direct regulator of CD47 through its 3'UTR. Furthermore, low miR-155 levels are associated with advanced stages of disease. MiR-155 overexpression suppressed CD47 expression on myeloma cell surface, leading to induction of phagocytosis of myeloma cells by macrophages and inhibition of tumor growth. MiR-155 overexpression also re-sensitized drug-resistant myeloma cells to bortezomib leading to cell death through targeting TNFAIP8, a negative mediator of apoptosis in vitro and in vivo. Thus, miR-155 mimics may serve as a promising new therapeutic modality by promoting phagocytosis and inducing apoptosis in patients with refractory/relapsed multiple myeloma.
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http://dx.doi.org/10.3324/haematol.2019.227579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716364PMC
December 2020

Sequential Doping of Ladder-Type Conjugated Polymers for Thermally Stable n-Type Organic Conductors.

ACS Appl Mater Interfaces 2020 Nov 12;12(47):53003-53011. Epub 2020 Nov 12.

Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, 60174 Norrköping, Sweden.

Doping of organic semiconductors is a powerful tool to optimize the performance of various organic (opto)electronic and bioelectronic devices. Despite recent advances, the low thermal stability of the electronic properties of doped polymers still represents a significant obstacle to implementing these materials into practical applications. Hence, the development of conducting doped polymers with excellent long-term stability at elevated temperatures is highly desirable. Here, we report on the sequential doping of the ladder-type polymer poly(benzimidazobenzophenanthroline) (BBL) with a benzimidazole-based dopant (i.e., N-DMBI). By combining electrical, UV-vis/infrared, X-ray diffraction, and electron paramagnetic resonance measurements, we quantitatively characterized the conductivity, Seebeck coefficient, spin density, and microstructure of the sequentially doped polymer films as a function of the thermal annealing temperature. Importantly, we observed that the electrical conductivity of N-DMBI-doped BBL remains unchanged even after 20 h of heating at 190 °C. This finding is remarkable and of particular interest for organic thermoelectrics.
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http://dx.doi.org/10.1021/acsami.0c16254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735673PMC
November 2020

Magnetizing lead-free halide double perovskites.

Sci Adv 2020 Nov 6;6(45). Epub 2020 Nov 6.

Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping SE-581 83, Sweden.

Spintronics holds great potential for next-generation high-speed and low-power consumption information technology. Recently, lead halide perovskites (LHPs), which have gained great success in optoelectronics, also show interesting magnetic properties. However, the spin-related properties in LHPs originate from the spin-orbit coupling of Pb, limiting further development of these materials in spintronics. Here, we demonstrate a new generation of halide perovskites, by alloying magnetic elements into optoelectronic double perovskites, which provide rich chemical and structural diversities to host different magnetic elements. In our iron-alloyed double perovskite, CsAg(Bi:Fe)Br, Fe replaces Bi and forms FeBr clusters that homogenously distribute throughout the double perovskite crystals. We observe a strong temperature-dependent magnetic response at temperatures below 30 K, which is tentatively attributed to a weak ferromagnetic or antiferromagnetic response from localized regions. We anticipate that this work will stimulate future efforts in exploring this simple yet efficient approach to develop new spintronic materials based on lead-free double perovskites.
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http://dx.doi.org/10.1126/sciadv.abb5381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673701PMC
November 2020

Sustainable biomass-based hierarchical porous carbon for energy storage: A novel route to maintain electrochemically attractive natural structure of precursor.

Sci Total Environ 2020 Dec 24;747:141923. Epub 2020 Aug 24.

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China; Jiangsu Engineering Research Center of Fast-growing Trees and Agri-fiber Materials, Nanjing 210037, China. Electronic address:

The development of sustainable and renewable energy storage devices with low cost and environment friendly features is an extremely urgent issue that needs to be solved. Herein, low-cost and sustainable biomass chitin, possessing natural fibrous, O/N-enriched and porous structure, was employed as a porous carbon (PC) precursor. However, a huge challenge in PC preparation is to maintain the natural electrochemically attractive structure of chitin while obtaining highly porous structure. In this study, by utilizing the molten protecting effect and micropore-creating ability of CuCl 2HO, the obtained PCs maintain the natural structure, achieve high yield (46%), and simultaneously develop hierarchical pores with a specific surface area range of 1635-2381 m g, a tunable micropore volume ratio range of 63.5-96.8%, and high surface O/N contents (N: 3.1-9.0 wt% and O: 10.5-12.8 wt%). Benefiting from these excellent properties, optimized PC achieves a high specific capacitance of 286 F g at 0.5 A g and a remarkably high rate capability of 88% at 10 A g; moreover, it even exhibits a rate capability of 80% at an ultrahigh current density of 50 A g. The optimized PC-based supercapacitor assembled in NaSO electrolyte shows a high energy density of 15.41 W h kg at 0.19 kW kg and achieves 76% energy density retention when the power density increased tenfold. Thus, this study presents a new way to fully utilize biomass, especially with electrochemically attractive natural structure, for developing advanced energy storage devices.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141923DOI Listing
December 2020

Self-assembled nanodisks in coaxial GaAs/GaAsBi/GaAs core-multishell nanowires.

Nanoscale 2020 Oct;12(40):20849-20858

Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.

III-V semiconductor nanowires (NWs), such as those based on GaAs, are attractive for advanced optoelectronic and nanophotonic applications. The addition of Bi into GaAs offers a new avenue to enhance the near-infrared device performance and to add new functionalities, by utilizing the remarkable valence band structure and the giant bowing in the bandgap energy. Here, we report that alloying with Bi also induces the formation of optically-active self-assembled nanodisks caused by Bi segregation. They are located in the vicinity to the 112 corners of the GaAsBi shell and are restricted to twin planes. Furthermore, the Bi composition in the disks is found to correlate with their lateral thickness. The higher Bi composition in the disks with respect to the surrounding matrix provides a strong confinement for excitons along the NW axis, giving rise to narrow emission lines (<450 μeV) with the predominant emission polarization orthogonal to the NW axis. Our findings, therefore, open a new possibility to fabricate self-assembled quantum structures by combining advantages of dilute bismide alloys and lattice engineering in nanowires.
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http://dx.doi.org/10.1039/d0nr05488gDOI Listing
October 2020

N-doped carbon-coated ultrasmall NbO nanocomposite with excellent long cyclability for sodium storage.

Nanoscale 2020 Sep 7;12(36):18673-18681. Epub 2020 Sep 7.

Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory for Novel Reactor and Green Chemistry Technology, Hubei Engineering Research Center for Advanced Fine Chemicals, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430205, P.R. China.

Niobium pentoxide (NbO) has drawn significant interest as a promising anode for sodium ion batteries (SIBs) due to its large interplanar lattice spacing and relatively high diffusion efficiency. However, the intrinsic drawbacks of low electrical conductivity and substantial volume change greatly impede its practical applications in large-scale energy storage systems. In this work, ultrasmall NbO nanoparticles wrapped with nitrogen-doped carbon (denoted as [email protected]) were delicately synthesized via a facile sol-gel method and subsequent heat treatment. The unique structure of ultrasmall NbO nanoparticles in a carbonaceous matrix can not only effectively shorten the transmission distance for both ions/electrons but also relieve the strain and stress caused by volume variation during the sodiation/desodiation process. In addition, the synergistic effect of nitrogen doping and carbon coating can further improve the electronic conductivity and pseudocapacitive behavior of the active materials, thus promoting the rapid electrochemical reaction kinetics of the [email protected] composite. The obtained [email protected] anode exhibits superior rate capability and outstanding cycling stability, delivering a reversible capacity of 196 mA h g at 1 A g after 1000 cycles. Even at high current densities of 5 A g and 10 A g, the long-life cycling tests show that the reversible capacities still remain at 128.4 mA h g and 95.9 mA h g after 3000 cycles, respectively, which is the best performance of NbO-based anodes at high current densities so far. These results indicate that the feasible synthetic strategy of [email protected] is an effective approach to develop high-performance NbO-based anodes for large-scale energy storage.
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http://dx.doi.org/10.1039/d0nr04922kDOI Listing
September 2020

Soil potassium is correlated with root secondary metabolites and root-associated core bacteria in licorice of different ages.

Plant Soil 2020 Sep 3:1-19. Epub 2020 Sep 3.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F University, 3 Taicheng Road, Yangling, Shaanxi 712100 People's Republic of China.

Aims: Licorice ( Fisch) is a crucial medicinal herb as it accumulates glycyrrhizin and liquiritin in roots. Licorice root-associated bacterial communities shaped by soil characteristics are supposed to regulate the accumulation of root secondary metabolites.

Methods: The soil characteristics, root secondary metabolites, and root-associated bacterial communities were analyzed in licorice plants of different ages to explore their temporal dynamics and interaction mechanisms.

Results: Temporal variation in soil characteristics and root secondary metabolites was distinct. The alpha-diversity of root-associated bacterial communities decreased with root proximity, and the community composition was clustered in the rhizosphere. Different taxa that were core-enriched from the dominant taxa in the bulk soil, rhizosphere soil, and root endosphere displayed varied time-decay relationships. Soil total potassium (TK) as a key factor regulated the temporal variation in some individual taxa in the bulk and rhizosphere soils; these taxa were associated with the adjustment of root secondary metabolites across different TK levels.

Conclusions: Licorice specifically selects root-associated core bacteria over the course of plant development, and TK is correlated with root secondary metabolites and individual core-enriched taxa in the bulk and rhizosphere soils, which may have implications for practical licorice cultivation.
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http://dx.doi.org/10.1007/s11104-020-04692-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468178PMC
September 2020

Stochastic processes shape the biogeographic variations in core bacterial communities between aerial and belowground compartments of common bean.

Environ Microbiol 2021 02 21;23(2):949-964. Epub 2020 Sep 21.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Although studies of biogeography in soil bacterial communities have attracted considerable attention, the generality of these patterns along with assembly processes and underlying drivers is poorly understood in the inner tissues of plants. Plant tissues provide unique ecological habitats for microorganisms, which play an essential role in plant performance. Here, we compared core bacterial communities among five soil-plant associated compartments of common bean across five sampling sites in China. Neutral and null modelling consistently suggested that stochastic processes dominated the core community assembly processes and escalated from the belowground compartments to the inner tissues of aerial plant parts. The multiple distance-decay relationships also varied and had flattened patterns in the stem endosphere, which were shaped by distinct environmental factors in each compartment. Coexistence patterns also varied in topological features, in addition with the sparsest networks in the stem endosphere resulted from the interaction with the stochastic processes. This study considerably expanded our understanding of various biogeographic patterns, assembly processes, and the underlying mechanisms of core bacterial communities between aerial and belowground compartments of common bean. That will provide a scientific basis for the reasonable regulation of core bacterial consortia to get better plant performance.
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http://dx.doi.org/10.1111/1462-2920.15227DOI Listing
February 2021

Production of lignin-containing cellulose nanofibers using deep eutectic solvents for UV-absorbing polymer reinforcement.

Carbohydr Polym 2020 Oct 6;246:116548. Epub 2020 Jun 6.

Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037 China. Electronic address:

Lignin-containing cellulose nanofibers (LCNFs) from energy cane bagasse (ECB), were prepared using microwave assisted deep eutectic solvent (MV-DES) treatment in combination with ultrasonication. The yield of lignocellulose is up to 45.2 % with 81.0 % delignification under the optimal reaction condition (110 ℃, 30 min). The resulting LCNF exhibited a highly entangled network, which was caused by the binder role of lignin between cellulose nanofibers. The addition of LCNFs improved the stability of the polyanionic cellulose (PAC) film-forming suspension, which was confirmed by the increased zeta potential and viscosity values. The LCNF / PAC films showed tunable mechanical and UV-resistant properties, depending on the amount and type of LCNFs. PAC films with the addition of 5 % LCNFs (PEF-5 %) showed good mechanical properties (a tensile strength of 55.8 MPa with a 26.3 % strain to break) and high UV protection ability (a UV-transmittance of 2.9 %).
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http://dx.doi.org/10.1016/j.carbpol.2020.116548DOI Listing
October 2020

Dietary phosphorus intake and serum prostate-specific antigen in non-prostate cancer American adults: A secondary analysis of the National Health and Nutrition Examination Survey (NHANES), 2003-2010.

Asia Pac J Clin Nutr 2020 ;29(2):322-333

Department of Urology, Guizhou Provincial People's Hospital, Guiyang, Guizhou Province, China. Email:

Background And Objectives: Previous study has reported phosphorus intake is associated prostate cancer (PCa), but the association between phosphorus intake and serum prostate specific antigen (PSA) levels hasn't been reported in non-history of PCa population. Therefore, we performed a secondary data analysis based on existing data from the public Nutrition Examination Survey (NHANES) (2003-2010) database.

Methods And Study Design: Totally 6403 participants were selected from NHANES (2003-2010) database. The interested independent and dependent variables were considered as dietary phosphorus intake and PSA level, respectively. Covariates included demographic data, dietary data, physical examination data, and comorbidities. Weighted linear regression and generalized additive models were used to addressing the linear and non-linear link of phosphorus intake to PSA level.

Results: Linear association between phosphorus intake and PSA was not detected [β=0.016 (95% Confidence Interval (CI) -0.012, 0.045)]. But we found an existing nonlinearity. By the recursive algorithm, the inflection point was 1151 mg. On the left side of the inflection point, we did not find the correlation between dietary phosphorus intake (per 100 change) and PSA level [β=-0.04 (95% CI -0.11, 0.02), p=0.2155], while dietary phosphorus intake (per 100 change) positively associated with PSA [β=0.05 (95% CI 0.01, 0.09) p=0.0293] on the right side of inflection point.

Conclusions: There is a non-linear correlation between dietary phosphorus intake and PSA. Dietary phosphorus intake was positively associated with increased PSA when dietary phosphorus intake is beyond 1151 mg after adjusting other covariates. Over 1151 mg per day dietary phosphorus intake may be the risk factor for PSA increasing.
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http://dx.doi.org/10.6133/apjcn.202007_29(2).0015DOI Listing
May 2021

Improving performance of recently introduced flow cytometry-based approach of malignant cell screening in serous cavity effusion.

Int J Lab Hematol 2020 Oct 23;42(5):612-618. Epub 2020 Jun 23.

Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Introduction: Microscopy has been recognized as the "gold-standard" cellular analysis of serous cavity effusion. However, this method is time-consuming, labor-intensive, and requires accomplished skills. Here, we investigated the efficiency of hematology analyzer in screening malignant cells in serous cavity effusion.

Methods: A total of 991 serous cavity effusion samples and 370 validation specimens collected from different departments were sent to the clinical laboratory for routine cell count using the automated hematology body fluid (BF) mode and exfoliative cytology simultaneously. High-fluorescent cells (HFCs) were measured as the relative count (HF%) and absolute count (HF#) by BF mode. Receiver operating characteristic curve analysis was combined with scattergram rules to screen malignant cells.

Results: HF# and HF% in malignant samples (subgroup) were significantly higher than those in benign samples, and the HF# and HF% levels were different between ascites and pleural effusion (PE). The area under the curve values were also different between ascites and PE. Positive of malignant cells was very high when the ascites or PE sample touching Rule 1 positive and either Rule 2 negative or positive. The cutoff levels of HF# were 5.5 HFC/μL on the basis of Rules 1 and 2 negative, whereas 83.5 HFC/μL on the basis of Rule 1 negative but Rule 2 positive in ascites. By contrast, the cutoff levels of HF% were 0.55 HFC/100 WBC on the basis of Rules 1 and 2 negative, whereas 4.95 HFC/100 WBC on the basis of Rule 1 negative but Rule 2 positive in PE.

Conclusions: Serous cavity effusion will be increasingly analyzed using the automated hematology analyzer BF mode in the future because of its rapidness and convenience. The combined application of HFC with scattergram rules is a feasible and useful approach to screen malignant cells in serous cavity effusion.
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http://dx.doi.org/10.1111/ijlh.13269DOI Listing
October 2020

Bone marrow mesenchymal stem cells improve thymus and spleen function of aging rats through affecting P21/PCNA and suppressing oxidative stress.

Aging (Albany NY) 2020 06 19;12(12):11386-11397. Epub 2020 Jun 19.

Provincial Clinical Medical College of Fujian Medical University, Fuzhou 350001, Fujian, China.

Bone marrow mesenchymal stem cells (BMSCs) have been considered to be an important regulator for immune function. We aim to prove the function improvement of aging spleen and thymus induced by BMSCs and unfold the specific mechanisms. Aging animal model was established using D-galactose. The morphological changes of spleen and thymus tissues were observed using hematoxylin-eosin staining and transmission electron microscopy. Key cytokines in the serum were measured with enzyme linked immunosorbent assay. Protein and mRNA levels of P16, P21, and PCNA were detected using western blotting and RT-qPCR. Special markers of BMSCs were identified using flow cytometry, and successful induction of BMSCs to steatoblast and osteoblasts was observed. Compared to aging model, BMSCs significantly increased the spleen and thymus index, improved the histological changes of spleen and thymus tissues. A remarkable increase of ratio between CD4+T cells and CD8+T cells, level of IL-2 was achieved by BMSCs. However, BMSCs markedly inhibited the content of IL-10, TNF-, P16, and P21 but promoted PCNA. Significant inhibition of oxidative stress by BMSCs was also observed. We demonstrated that BMSCs significantly improved the tissue damage of aging spleen and thymus, BMSCs may improve aging organs through influencing cytokines, oxidative stress, and P21/PCNA.
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http://dx.doi.org/10.18632/aging.103186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343510PMC
June 2020

Proteome of the fungus Phoma macdonaldii, the causal agent of black stem of sunflower.

J Proteomics 2020 08 12;225:103878. Epub 2020 Jun 12.

Xinjiang Yili Vocational Technical College, Yining 835000, China.

Phoma macdonaldii causes black stem of sunflower, which severely affects sunflower yield and quality. There is currently little molecular information available for this pathogenic fungus. In this study, a global proteomic analysis of P. macdonaldii was performed to determine the biological characteristics and pathogenicity of this pathogen. A total of 1498 proteins were identified by LC-MS/MS in all biological replicates. Among the identified proteins, 1420 proteins were classified into the three main GO categories (biological process, cellular component, and molecular function) while 806 proteins were annotated into the five major KEGG database (metabolism, genetic information processing, environmental information processing, cellular processes, and organismal systems). The regulated expression levels of eight genes encoding selected identified proteins were investigated to assess their potential effects on fungal development and pathogenesis. To the best of our knowledge, this is the first study to characterize the proteome of the necrotrophic fungus P. macdonaldii. The presented results provide novel insights into the development and pathogenesis of P. macdonaldii and possibly other Phoma species. SIGNIFICANCE: Black stem of sunflower is a devastating disease caused by the necrotrophic fungus Phoma macdonaldii. Relatively little is known regarding the molecular characteristics of this pathogen, and no proteomic investigation has been reported. Thus, we conducted a global proteomic analysis of P. macdonaldii. Many proteins were found to be differentially regulated during fungal development and pathogenesis, suggesting they may be important for these two processes. This is the first proteomic study of P. macdonaldii, and the data presented herein will be useful for elucidating the molecular characteristics of this fungus as well as other Phoma species.
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http://dx.doi.org/10.1016/j.jprot.2020.103878DOI Listing
August 2020
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