Publications by authors named "Weilie Zheng"

7 Publications

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Genetic diversity and population structure of two endemic (Cupressaceae) species on the Qinghai-Tibetan plateau.

J Genet 2019 03;98

Tibet Agricultural and Animal Husbandry College, Nyingchi 860000, Tibet, People's Republic of China.

and are ecologically and economically important endemic species of the conifer family Cupressaceae on the Qinghai-Tibetan plateau. was previously classified as a subspecies of because of their similar morphological characteristics and close distribution. In this study, 401 individuals were sampled from 16 populations of the two species. The specimens were genotyped using 10 polymorphic microsatellite loci through fluorescence polymerase chain reaction (PCR). The genetic diversity of and populations was generally low, with the highest genetic diversity detected in the population LLS of . Distance-based phylogenetic and principal co-ordinates analyses indicated a clear genetic structures for the 16 populations of the two species. Moreover, Mantel test results showed indistinctive correlations between population-pairwise F values and geographic distances, as well as between genetic distances and geographic distances in and , respectively. AMOVA suggested that genetic variation mostly resided within populations. Sixteen naturalpopulations were evidently clustered into two major groups in the constructed neighbour-joining tree. The results demonstrated that and are different species. The genetic information provided important theoretical references for conservation and management of the two endangered Cupressus species.
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March 2019

Integrated Analysis of Transcriptomic and Proteomics Data Reveals the Induction Effects of Rotenoid Biosynthesis of Caused by UV-B Radiation.

Int J Mol Sci 2018 Oct 25;19(11). Epub 2018 Oct 25.

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

(Edgew.) Heimerl is one of the most important genuine medicinal plants in Tibet, in which the special plateau habitat has been associated with its excellent medicinal quality and efficacy. However, the mechanisms by which environmental factors affect biosynthesis of secondary metabolic components remain unclear in this species. In this study, RNA sequencing and iTRAQ (isobaric Tags for Relative and Absolute Quantification) techniques were used to investigate the critical molecular "events" of rotenoid biosynthesis responding to UV-B radiation, a typical plateau ecological factor presented in native environment-grown plants. A total of 3641 differentially expressed genes (DEGs) and 106 differentially expressed proteins (DEPs) were identified in between UV-B treatment and control check (CK). Comprehensive analysis of protein and transcript data sets resulted in 14 and 7 DEGs from the plant hormone signal transduction and phosphatidylinositol signaling system pathways, respectively, being significantly enriched. The result showed that the plant hormone signal transduction and phosphatidylinositol signaling system might be the key metabolic strategy of UV-B radiation to improve the biosynthesis of rotenoid in . At same time, most of the DEGs were associated with auxin and calcium signaling, inferring that they might drive the downstream transmission of these signal transduction pathways. Regarding those pathways, two chalcone synthase enzymes, which play key roles in the biosynthesis of rotenoid that were thought as the representative medicinal component of , were significantly upregulated in UV-B radiation. This study provides a theoretical basis for further exploration of the adaptation mechanism of to UV-B radiation, and references for cultivation standardization.
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http://dx.doi.org/10.3390/ijms19113324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274831PMC
October 2018

Characterization of the complete chloroplast genome of Juniperus cedrus (Cupressaceae).

Mitochondrial DNA A DNA Mapp Seq Anal 2016 11 14;27(6):4355-4356. Epub 2015 Oct 14.

a Agricultural and Animal Husbandry College, Tibet University , Nyingchi , Tibet , China.

The complete chloroplast genome of Juniperus cedrus is a circular molecule of 127 126 bp in length with 115 single copy genes and two duplicated genes (trnI-CAU and trnQ-UUG). The genome contains 82 protein-coding genes, four ribosomal RNA genes and 33 transfer RNA genes. In these genes, eight genes (rpl16, rpl2, rpoC1, petD, petB, ndhB, ndhA and atpF) harbor a single intron and two genes (rps12 and ycf3) harbor two introns. Like other sequenced chloroplast genomes of conifers, this genome does not contain canonical inverted repeats (IRs), and the overall GC content of J. cedrus chloroplast DNA is 35%. The phylogenetic analysis revealed that J. cedrus is more closely related to J. scopulorum and J. bermudiana.
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http://dx.doi.org/10.3109/19401736.2015.1089498DOI Listing
November 2016

The complete chloroplast genome of Cupressus gigantea, an endemic conifer species to Qinghai-Tibetan Plateau.

Mitochondrial DNA A DNA Mapp Seq Anal 2016 09 10;27(5):3743-4. Epub 2015 Sep 10.

a Agricultural and Animal Husbandry College, Tibet University , Nyingchi , Tibet , China .

The complete chloroplast genome of the wild Cupressus gigantea (Cupressaceae) is determined in this study. The circular genome is 128 244 bp in length with 115 single copy genes and two duplicated genes (trnI-CAU and trnQ-UUG). This genome contains 82 protein-coding genes, four ribosomal RNA genes and 31 transfer RNA genes. In these genes, eight genes (atpF, rpoC1, ndhA, ndhB, petB, petD, rpl16 and rpl2) harbor a single intron and two genes (rps12 and ycf3) harbor two introns. This genome does not contain canonical IRs, and the overall GC content is 34.7%. A maximum parsimony phylogenetic analysis revealed that C. gigantea and C. sempervirens are more closely related.
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http://dx.doi.org/10.3109/19401736.2015.1079885DOI Listing
September 2016

Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica.

Biotechnol Appl Biochem 2016 May 5;63(3):419-26. Epub 2015 Nov 5.

TAAHC-SWU Medicinal Plant Joint R&D Centre, Agricultural and Animal Husbandry College, Tibet University, Nyingchi of Tibet, People's Republic of China.

Mirabilis himalaica is an endangered medicinal plant species in the Tibetan Plateau. The two genes respectively encoding chalcone synthase (MhCHS) and chalcone isomerase (MhCHI) were isolated and characterized from M. himalaica. The sequence analysis revealed that the two genes were similar with their corresponding homologous genes in other plants. The tissue profiles showed that both MhCHS and MhCHI had higher expression levels in roots than in stems and leaves. Transgenic hairy root cultures respectively with overexpressing MhCHS and MhCHI were established. The genomic PCR detection confirmed the authority of transgenic hairy root lines, in which either MhCHS or MhCHI expression levels were much higher than that in non-transgenic hairy root line. Finally, the HPLC detection results demonstrated that the rotenoid contents in MhCHS/MhCHI-transformed hairy root lines were enhanced. This study provided two candidate genes that could be used to genetic engineering rotenoid biosynthesis in M. himalaica and an alternative method to produce rotenoid using transgenic hairy root cultures.
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http://dx.doi.org/10.1002/bab.1376DOI Listing
May 2016

Engineering salidroside biosynthetic pathway in hairy root cultures of Rhodiola crenulata based on metabolic characterization of tyrosine decarboxylase.

PLoS One 2013 4;8(10):e75459. Epub 2013 Oct 4.

Agricultural and Animal Husbandry College, Tibet University, Nyingchi of Tibet, P. R. China ; Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Engineering and Technology Research Center for Sweetpotato, School of Life Sciences, Southwest University, Chongqing, P. R. China.

Tyrosine decarboxylase initializes salidroside biosynthesis. Metabolic characterization of tyrosine decarboxylase gene from Rhodiola crenulata (RcTYDC) revealed that it played an important role in salidroside biosynthesis. Recombinant 53 kDa RcTYDC converted tyrosine into tyramine. RcTYDC gene expression was induced coordinately with the expression of RcUDPGT (the last gene involved in salidroside biosynthesis) in SA/MeJA treatment; the expression of RcTYDC and RcUDPGT was dramatically upregulated by SA, respectively 49 folds and 36 folds compared with control. MeJA also significantly increased the expression of RcTYDC and RcUDPGT in hairy root cultures. The tissue profile of RcTYDC and RcUDPGT was highly similar: highest expression levels found in stems, higher expression levels in leaves than in flowers and roots. The gene expressing levels were consistent with the salidroside accumulation levels. This strongly suggested that RcTYDC played an important role in salidroside biosynthesis in R. crenulata. Finally, RcTYDC was used to engineering salidroside biosynthetic pathway in R. crenulata hairy roots via metabolic engineering strategy of overexpression. All the transgenic lines showed much higher expression levels of RcTYDC than non-transgenic one. The transgenic lines produced tyramine, tyrosol and salidroside at higher levels, which were respectively 3.21-6.84, 1.50-2.19 and 1.27-3.47 folds compared with the corresponding compound in non-transgenic lines. In conclusion, RcTYDC overexpression promoted tyramine biosynthesis that facilitated more metabolic flux flowing toward the downstream pathway and as a result, the intermediate tyrosol was accumulated more that led to the increased production of the end-product salidroside.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075459PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790822PMC
July 2014

[Biomass and yield of Betula platyphylla population in Mila Mount of Tibet].

Ying Yong Sheng Tai Xue Bao 2004 Aug;15(8):1329-33

Institute of Tibet Plateau Ecology, Linzhi, China.

Mila Mount in Southeast Tibet is the west boundary of the horizontal distribution of Betula platyphylla. It is also the highest altitude area in China and the world, where Betula platyphylla is distributed. Based on the investigation of regional sample plots and the analysis of analytic trees, this paper compared the biomass and yield of Betula platyphylla population at different slope orientation and different altitude. The results showed that the growth of Betula platyphylla was greatly interfered by human activities. The yearly productivity of Betula platyphylla grown in Mila Mount was 363.1-2,072.94 kg x hm(-2), and the biomass was 7,625.00-33,167.00 kg x hm(-2), indicating that the biomass of Betula platyphylla was quite different for different population. The productivity and biomass of Betula platyphylla coppice after cutting were lower than those of seedling forest, and there existed youth broken-down in the coppice. The zonal changes of water resources had no significant effect on the water allocation and the biomass of Betula platyphylla.
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August 2004