Publications by authors named "Weijie Dai"

20 Publications

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Physiological responses and health risks of edible amaranth under simultaneous stresses of lead from soils and atmosphere.

Ecotoxicol Environ Saf 2021 Oct 28;223:112543. Epub 2021 Jul 28.

Guangdong Research Center for Industrial Contaminated Site Remediation Technology and Equipment Engineering, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, PR China.

Lead (Pb) is widely distributed in the environment that can impose potential risks to vegetables and humans. In this work, we conducted a pot experiment in Southern China to examine the physiological response and risk of edible amaranth (Amaranthus tricolor L.) under the simultaneous stresses of lead from soil and atmosphere. The results indicate that the lead content of amaranth substantially exceeded China's national standard when Pb concentration from soils and atmosphere was high, and comparing to teenagers and adults, children exposed a higher health risk after consuming the contaminated amaranth. Under the co-stress, the lead in roots of amaranth mainly came from the soil, but the Pb from atmospheric deposition can significantly affect the lead concentration in leaves. While lead from atmospheric deposition is found to promote the growth of amaranth, the stress of lead from the soils shows an inhibitory effect, as indicated by the increase in HO content, the damage in cell membranes, and the limitation in chlorophyll synthesis. The antioxidant system in stems and leaves of amaranth can effectively alleviate the Pb toxicity. However, the stress of high lead concentration from soils can substantially suppress the antioxidant enzyme activity of roots. While it is found that heavy metals in soils can significantly affect the vegetables grown in a multi-source pollution environment, we also call for the attention on the potential health risk imposed by the lead from atmospheric deposition. This study provides an important reference for the prevention and control of crop contamination in multi-source pollution environments.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112543DOI Listing
October 2021

ELK1‑mediated upregulation of lncRNA LBX2‑AS1 facilitates cell proliferation and invasion via regulating miR‑491‑5p/S100A11 axis in colorectal cancer.

Int J Mol Med 2021 Jul 3;48(1). Epub 2021 Jun 3.

Division of Gastroenterology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

The aim of the present study was to investigate the role and regulatory mechanism of LBX2 antisense RNA 1 (LBX2‑AS1) in colorectal cancer. Firstly, LBX2‑AS1 expression was detected using reverse transcription‑quantitative PCR in colorectal cancer tissues and cells, and its prognostic and diagnostic efficacy was assessed in a colorectal cancer cohort (n=145). Subcellular fractionation assay of LBX2‑AS1 was performed. Secondly, the effects of LBX2‑AS1 and microRNA (miR)‑491‑5p on colorectal cancer cell proliferation, apoptosis, migration and invasion were investigated by a series of functional assays. Thirdly, RNA immunoprecipitation, dual‑luciferase reporter and gain and loss of function assays were carried out to analyze the interactions between ETS transcription factor ELK1 (ELK1) and LBX2‑AS1, as well as LBX2‑AS1, miR‑491‑5p and S100A11. The results showed that LBX2‑AS1 was upregulated both in colorectal cancer tissues and cells, which was distributed in the cytoplasm and nucleus of colorectal cancer cells. Clinically, high LBX2‑AS1 expression could be an independent prognostic factor for colorectal cancer. Furthermore, relative operating characteristic curve analysis showed that LBX2‑AS1 was a sensitive diagnostic marker for colorectal cancer. Highly expressed ELK1, as a transcription factor, could bind to the two conserved sites in the promoter region of LBX2‑AS1, thereby activating the transcription of LBX2‑AS1. Silencing LBX2‑AS1 markedly inhibited proliferative, migratory and invasive abilities of colorectal cancer cells. miR‑491‑5p expression was downregulated, while S100A11 expression was upregulated in colorectal cancer tissues and cells. Dual‑luciferase reporter assays confirmed that LBX2‑AS1 could block S100A11 degradation via competitively binding to miR‑491‑5p. Furthermore, LBX2‑AS1 overexpression could notably reverse the inhibitory effect of miR‑491‑5p on proliferation and invasion of colorectal cancer cells. Taken together, LBX2‑AS1 induced by transcription factor ELK1 may facilitate colorectal cancer cell proliferation and invasion via regulation of the miR‑491‑5p/S100A11 axis. Thus, LBX2‑AS1 could be an underlying prognostic and diagnostic marker for colorectal cancer.
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http://dx.doi.org/10.3892/ijmm.2021.4971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175069PMC
July 2021

Fermented Duckweed as a Potential Feed Additive with Poultry Beneficial Bacilli Probiotics.

Probiotics Antimicrob Proteins 2021 May 14. Epub 2021 May 14.

Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA.

In this study, the duckweed varieties Lemna minor, Spirodela polyrhiza, and a commercially processed duckweed food supplement were investigated as potential substrates for the propagation of two probiotic Bacillus strains, B. subtilis KATMIRA1933 and B. amyloliquefaciens B-1895. Both L. minor and S. polyrhiza were found to be suitable substrates for the propagation of both bacilli, with 8.47-9.48 Log CFU/g and 10.17-11.31 Log CFU/g after 24 and 48 h growth on the substrates, respectively. The commercial duckweed product was a less favorable substrate, with growth reaching a maximum of 7.89-8.91 CFU/g after 24 h with no further growth after 48 h. Growth and adherence of the bacilli to the three products were confirmed via electron microscopy. These strains have demonstrated health-promoting benefits for poultry and thereby have the potential to enhance duckweed as an animal feed through the process of fermentation. Duckweed has been shown to be a promising alternative resource for protein and has the opportunity to become a valuable resource in multiple industries as a potential means to increase sustainability, food security, and reduce environmental impact.
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http://dx.doi.org/10.1007/s12602-021-09794-4DOI Listing
May 2021

Cadmium accumulation and physiological response of Amaranthus tricolor L. under soil and atmospheric stresses.

Environ Sci Pollut Res Int 2021 Mar 17;28(11):14041-14053. Epub 2020 Nov 17.

Department of Geography, Florida State University, Tallahassee, FL, 32306, USA.

In this study, cadmium (Cd) solution spraying and Cd-contaminated soil pot experiments were conducted to investigate the influence of Cd from atmospheric deposition and soil on the growth, cumulative distribution, chemical morphology, physiological, and biochemical responses of Amaranthus tricolor L. The results indicated that Cd in plants mainly came from soil (92-98%) and was stored in the roots in large quantities while the portion from atmospheric deposition could also effectively increase Cd content in stems and leaves (2-3%). Cd was mainly stored in plant cell walls and would transfer to the soluble part under high-concentration soil stress Cd from atmospheric deposition alone promoted the growth of plants, but high Cd concentrations from soil had the negative influence. The contents of HO and MDA in plants increased under soil and atmospheric Cd stress, indicating that the plant cells were damaged by oxidative stress. The content of antioxidant enzymes such as POD, CAT, SOD, and antioxidants like AsA and GSH increased under low-concentration Cd stress but decreased under elevated stress, suggesting that high Cd-contaminated soil poses severe toxicity on the antioxidant system of the plants. Hence, the accumulation and physiological response of plants under multi-source Cd contamination were mainly affected by high soil Cd concentrations. Though the effect of atmospheric deposition is relatively less, it cannot be ignored.
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http://dx.doi.org/10.1007/s11356-020-11569-3DOI Listing
March 2021

Long non‑coding RNA BANCR mediates esophageal squamous cell carcinoma progression by regulating the IGF1R/Raf/MEK/ERK pathway via miR‑338‑3p.

Int J Mol Med 2020 Oct 28;46(4):1377-1388. Epub 2020 Jul 28.

Department of Digestive Endoscopy, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.

Esophageal squamous cell carcinoma (ESCC) is a type of digestive tract malignant tumor that severely threatens human health. The long non‑coding RNA BRAF activated non‑coding RNA (BANCR) and insulin‑like growth factor 1 receptor (IGF1R) are associated with various types of cancer; however, it remains unclear whether BANCR can regulate IGF1R expression in ESCC. In the present study, the expression levels of BANCR, IGF1R mRNA and microRNA‑338‑3p (miRNA/miR‑338‑3p) in ESCC tissues or cells were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The levels of IGF1R, E‑cadherin, N‑cadherin, Vimentin, p‑Raf‑1, p‑MEK1/2 and p‑ERK1/2 were measured by western blot analysis. The proliferation, migration and invasion of ESCC cells were determined by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) or Transwell assays. The relationship between miR‑338‑3p and BANCR or IGF1R was predicted using starBase2.0 and confirmed by dual‑luciferase reporter assay. The role of BANCR in ESCC in vivo was confirmed through a tumor xenograft assay. It was found that BANCR and IGF1R were upregulated, while miR‑338‑3p was downregulated in ESCC tissues and cells. Both BANCR and IGF1R knockdown suppressed the proliferation, migration, invasion and epithelial‑mesenchymal transition (EMT) of ESCC cells. IGF1R enhancement reversed BANCR knockdown‑mediated effects on the proliferation, migration, invasion and EMT of ESCC cells. BANCR regulated the Raf/MEK/ERK pathway by regulating IGF1R expression. Notably, BANCR regulated IGF1R expression by sponging miR‑338‑3p. Moreover, BANCR silencing inhibited tumor growth in vivo. On the whole, the findings of the present study demonstrate that BANCR inhibition blocks ESCC progression by inactivating the IGF1R/Raf/MEK/ERK pathway by sponging miR‑338‑3p.
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http://dx.doi.org/10.3892/ijmm.2020.4687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447317PMC
October 2020

Immobilization of Aspergillus niger lipase onto a novel macroporous acrylic resin: Stable and recyclable biocatalysis for deacidification of high-acid soy sauce residue oil.

Bioresour Technol 2020 Feb 7;298:122553. Epub 2019 Dec 7.

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. Electronic address:

Deacidification of high-acid soy sauce residue (SSR) oil is crucial to utilization of SSR oil. Aspergillus niger lipase (ANL) has been widely applied for such purpose while its immobilization still has large room for improvement. ANL was immobilized onto six different macroporous acrylic resins, accounting the effect of the different textural properties of resins on stability and their potential for application in enzymatic deacidification. The resin MARE with lower porosity, higher bulk density, and medium hydrophobicity, was chosen as the best carrier for the best thermostability and reusability. ANL-MARE is a promising catalyst than Novozym 40086, which not only exhibited higher deacidification activity and good thermostability, but also was continuously reused for 15 cycles and efficiently catalyzed from high-acid SSR oil into diacylglycerol-enriched oil. Therefore, immobilized ANL was a novel, low-cost and recyclable biocatalyst that could be used as a good alternative to higher-cost commercial lipases in industrial applications.
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http://dx.doi.org/10.1016/j.biortech.2019.122553DOI Listing
February 2020

miR-424-5p promotes the proliferation and metastasis of colorectal cancer by directly targeting SCN4B.

Pathol Res Pract 2020 Jan 11;216(1):152731. Epub 2019 Nov 11.

Department of Gastroenterology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu, 223300, China. Electronic address:

Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide usually diagnosed at advanced stages which causes poor prognosis of patients. Therefore, novel diagnostic biomarkers and therapeutic targets are urgently needed.

Materials And Methods: miR-424-5p was identified through integrated analysis of three public databases. Loss-of-function experiments in HT29 and SW480 cells and mouse xenograft models were performed to explore the regulatory role of miR-424-5p in CRC. Bioinformatics analysis was used for predicting targets of miR-424-5p and its functional and pathway enrichment analysis.

Results: miR-424-5p expression was significantly upregulated in CRC tissues and cell lines and associated with prognosis of CRC patients. Experiments in vitro and in vivo showed miR-424-5p promotes CRC cell proliferation and metastasis by directly inhibiting SCN4B. Besides, CRC cells secret miR-424-5p into peripheral blood through exosomes and circulating exosomal miR-424-5p could discriminate CRC patients with early stage from healthy people with AUC value of 0.82.

Conclusions: miR-424-5p serves as an oncogene in CRC and circulating exosomal miR-424-5p is a novel potential diagnostic biomarker of CRC patients.
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http://dx.doi.org/10.1016/j.prp.2019.152731DOI Listing
January 2020

Evaluation of an Industrial Soybean Byproduct for the Potential Development of a Probiotic Animal Feed Additive with Bacillus Species.

Probiotics Antimicrob Proteins 2020 09;12(3):1173-1178

Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences , Rutgers State University, New Brunswick, NJ, USA.

Probiotics are gaining public attention for their application in animal husbandry due to their ability to promote growth and prevent infections. Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 are two spore-forming probiotic microorganisms that have been demonstrated to provide health benefits for poultry when supplemented into their diet. These strains can be propagated on a wide range of substrates, including soybean-derived byproducts from the food processing industry. Soybean-derived byproducts are often incorporated into animal feeds, but the value of an additive could potentially be increased by the addition of probiotic microorganisms, which may decrease production costs and reduce environmental impact. In this study, a soybean byproduct and a desalted version of this byproduct were evaluated as potential substrates for the growth of two probiotic bacilli species. Chemical analysis of these byproducts showed favorable carbohydrate, fat, and amino acid profiles, which were not affected by the desalting process. The desalted byproduct was further evaluated as a substrate for the growth of B. subtilis KATMIRA1933 and B. amyloliquefaciens B-1895 under solid-state conditions, and samples from this experiment were visualized by scanning electron microscopy. The results of this study indicate that the desalted soybean byproduct is a suitable substrate for the propagation of the two Bacillus species, which grew to numbers sufficient for the formulation of a probiotic animal feed additive.
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http://dx.doi.org/10.1007/s12602-019-09619-5DOI Listing
September 2020

Overcoming Drug Resistance in Colon Cancer by Aptamer-Mediated Targeted Co-Delivery of Drug and siRNA Using Grapefruit-Derived Nanovectors.

Cell Physiol Biochem 2018 2;50(1):79-91. Epub 2018 Oct 2.

Huai'an Key Laboratory of Esophageal Cancer Biobank, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, China.

Background/aims: Multidrug resistance (MDR) is the most common cause of chemotherapy failure. Upregulation of P-glycoprotein (P-gp) is one of the main mechanisms underlying MDR.

Methods: In this study, we developed a targeted drug and small interfering (si)RNA co-delivery system based on specific aptamer-conjugated grapefruit-derived nanovectors (GNVs) that we tested in MDR LoVo colon cancer cells. The internalization of nanovectors in cancer cells was tested by fluorescence microscopy and flow cytometry. The anti-cancer activity in vitro was determined by colony formation and cell apoptosis assays. The biodistribution of nanovectors was analyzed by live imaging and the anti-cancer activity in vivo was observed.

Results: GNVs loaded with aptamer increased doxorubicin (Dox) accumulation in MDR LoVo cells, an effect that was abolished by pretreatment with DNase. The LA1 aptamer effectively promoted nanovector internalization into cells at 4°C and increased the targeted delivery of Dox to tumors. Constructs harboring Dox, LA1, and P-gp siRNA more effectively inhibited proliferation and enhanced apoptosis in cultured MDR LoVo cells while exhibiting more potent anti-tumor activity in vivo than free Dox or GNVs loaded with Dox alone or in conjunction with LA1, an effect that was associated with downregulation of P-gp expression.

Conclusion: This GNV-based system may be an effective strategy for overcoming MDR in clinical settings.
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http://dx.doi.org/10.1159/000493960DOI Listing
November 2018

Effect of GDF11 on proliferation and apoptosis of esophageal cancer cells.

Cell Mol Biol (Noisy-le-grand) 2018 08 30;64(11):80-84. Epub 2018 Aug 30.

Department of Thoracic Surgery, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China.

This study was aimed at investigating the effect of growth differentiation factor 11 (GDF11) on the proliferation and apoptosis of esophageal cancer cells. Serum levels of GDF11 in esophageal cancer patients were determined with ELISA kits, and the correlation between serum GDF11 and pathological features of esophageal cancer were determined. The effect of recombinant GDF11 on the growth of esophageal cancer cells was measured by CCK6 method. In order to investigate the effect of recombinant GDF11 on the proliferation and apoptosis of esophageal cancer cells, the expression of apoptosis-promoting protein Bax and proliferative-associated protein Bcl-2 in esophageal cancer cells were determined using western blot. Moreover, GDF11 was used to treat esophageal cancer cells, and its effect on proliferation and apoptosis was determined with MTT assay and flow cytometry, respectively. The serum content of GDF11 was much less in esophageal cancer patients than in the control group. Esophageal GDF II in cancer patients was correlated with cancer differentiation: the higher the degree of differentiation, the higher the content of GDF11. GDF11 inhibits proliferation and apoptosis of esophageal cancer cells.
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August 2018

Prognostic Implications of miR-302a/b/c/d in Human Gastric Cancer.

Pathol Oncol Res 2017 Oct 9;23(4):899-905. Epub 2017 Aug 9.

Department of Internal Medicine, Lianshui Third People's Hospital, 12 Gaogouzhen 307 Road South, Lianshui, Jiangsu, 223411, People's Republic of China.

Background: The microRNA (miR)-302 family consisting four members, miR-302a, miR-302b, miR-302c and miR-302d, plays an important role in diverse biological processes, and regulates many pathological changes, including cancer. However, the involvement of the miR-302 family into human gastric cancer (GC) remains unclear. The aim of this study was to investigate the expression patterns of miR-302a/b/c/d and determine their clinical significance in GC.

Materials And Methods: Expression levels of miR-302a/b/c/d in 160 pairs of human GC and matched normal mucosa tissues were detected by quantitative real-time polymerase chain reaction. Then, the associations of miR-302a/b/c/d expression with various clinicopathological characteristics and patients' prognosis were statistically evaluated.

Results: The expression levels of miR-302a, miR-302b and miR-302c in GC tissues were all significantly lower than those in matched normal mucosa (all P < 0.001), but miR-302d expression had no significant differences between cancer and normal groups. Additionally, GC patients with low miR-302a, miR-302b and miR-302c expression more frequently had positive lymph node metastasis (all P < 0.05), advanced TNM stage (all P < 0.05) and great depth of invasion (all P < 0.05). More importantly, low miR-302a, miR-302b and miR-302c expression in GC tissues were significantly associated with shorter disease-free and overall survivals of GC patients (all P < 0.05). Further multivariate analysis identified miR-302a, miR-302b and miR-302c as independent prognostic markers for GC patients.

Conclusions: GC patients with the decreased expression of miR-302a, miR-302b and miR-302c may had aggressive cancer progression and unfavorable prognosis. Further rigorous validation based on a large cohort of clinical cases should be performed.
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http://dx.doi.org/10.1007/s12253-017-0282-7DOI Listing
October 2017

Roles of ZIC family genes in human gastric cancer.

Int J Mol Med 2016 Jul 12;38(1):259-66. Epub 2016 May 12.

Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

The human zinc finger of the cerebellum (ZIC)family genes, comprised of 5 members, which are vertebrate homologues of the Drosophila odd-paired gene and encode zinc-finger transcription factors, have been shown to be involved in various diseases, including cancer. However, the roles of ZICs in human gastric cancer (GC) have not yet been fully elucidated. This study aimed to investigate the expression patterns of ZICs and determine their clinical significance in GC. The mRNA and protein expression levels of ZIC1-5 were detected by RT-qPCR and western blot analysis, respectively using 60 pairs of human GC and matched normal mucosa tissues. The expression pattern and subcellular localization of ZIC1 in 160 pairs of human GC and matched normal mucosa tissues were verified by immunohistochemistry. Moreover, the associations of ZIC1 expression with various clinicopathological characteristics and patient prognosis were evaluated. The mRNA and protein expression levels of ZIC1 were both found to be significantly decreased in the GC tissues compared to matched normal mucosa tissues (GC vs. normal, 2.15±0.69 vs. 4.28±0.95; P<0.001); however, ZIC2-5 expression exhibited no significant difference between the cancer and normal tissue samples. In addition, the downregulation of ZIC1 (ZIC1-low) was more frequently observed in the GC tissues with positive lymph node metastasis (P=0.006), an advanced TNM stage (P<0.001) and a great depth of invasion (P=0.01). Notably, a low ZIC1 expression was significantly associated with a poor disease-free and overall survival. Furthermore, multivariate analysis revealed that ZIC1 expression was an independent prognostic marker for patients with GC. In conclusion, among the human ZIC family genes, the dysregulation of ZIC1, but not of ZIC2, ZIC3, ZIC4 and ZIC5, may play a crucial role in the progression of GC. ZIC1 may thus serve as a novel molecular marker to predict the progression, survival and relapse of patients with GC.
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http://dx.doi.org/10.3892/ijmm.2016.2587DOI Listing
July 2016

Association of Serum Uric Acid with Body Mass Index: A Cross-Sectional Study from Jiangsu Province, China.

Iran J Public Health 2014 Nov;43(11):1503-9

2. Dept. of Medical Centerfor Digestive Diseases, the Second Affiliated Hospital of Nanjing Medical University , Nanjing, P.R. China.

Background: Body mass index (BMI) has been demonstrated to be associated with serum uric acid (SUA) level in many developed countries, however, there is still a lack of large sample study in Jiangsu Province, one of the most economically developed regions in China, where fat-rich diet is common.

Methods: Through retrospective analysis in healthy subjects, we determined the association of BMI with hyperuricemia risk. Data of 39,736 participants from January 2011 to June 2013 in China were analyzed for parameters including physical examinations and biochemical blood analysis.

Results: On univariate analysis, SUA was positively correlated with age, SBP, DBP, BMI, FPG, red blood cell count, hemoglobin, white blood cell count, platelet, cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, ALT, AST, bilirubin, albumin, BUN and creatinine. SUA was significantly elevated in a linear fashion as BMI increased, and SUA in obesity was significantly higher than underweight. The prevalence of hyperuricemia remained approximately 2.98 times greater among individuals with overweight, and 5.96 times greater among obesity, compared to individuals with underweight.

Conclusion: There is a positive relationship between BMI and SUA among healthy subjects in Jiangsu province, China.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449499PMC
November 2014

Psychodynamic and biodynamic analysis of treatment of outlet obstructive constipation (OOC) using Procedure for Prolapse and Hemorrhoids (PPH).

Med Hypotheses 2015 Jul 28;85(1):58-60. Epub 2015 Mar 28.

Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, PR China. Electronic address:

To discuss the possible pathogenesis of outlet obstructive constipation (OOC) and identify the theoretical basis of the Procedure for Prolapse and Hemorrhoids (PPH) used to treat outlet obstructive constipation (OOC). 19 patients diagnosed with outlet obstructive constipation (OOC) form the case group, and 9 healthy volunteers form the control group. Patients, before and after operation, and the control group, were equally given such tests as Hamilton Depression Rating Scale (HAMD), Hamilton Anxiety Scale (HAMA) and anorectal dynamics. No significant difference in the functional lengths of anal canals was found between all groups (F = 0.98, p = 0.41). The minimum perception threshold, maximum tolerance threshold, and rectal defecation threshold of Group A, of 15 days after operation, were equally lower than those before operation, and than the control group (P < 0.05). These thresholds rebounded significantly in Group B 90 days after operation. Mentally, HAMA (F = 23.75, p = 0.00) and HAMD (F = 20.99, p = 0.00) total scores, after operation, were equally decreased first and then rebounded. Patients with outlet obstructive constipation (OOC) are subject to anorectal dynamic disorders as well as mental and psychological disorders, which can be remarkably improved using the Procedure for Prolapse and Hemorrhoids (PPH).
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http://dx.doi.org/10.1016/j.mehy.2015.03.022DOI Listing
July 2015

Upregulation of microRNA-23a/b promotes tumor progression and confers poor prognosis in patients with gastric cancer.

Int J Clin Exp Pathol 2014 1;7(12):8833-40. Epub 2014 Dec 1.

Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, P. R. China.

Background And Purpose: To investigate the clinical significance of microRNA (miR)-23a and miR-23b expression in human gastric cancer (GC).

Methods: Quantitative RT-PCR was performed to detect the expression changes of miR-23a and miR-23b in 160 human GC tissues and paired normal mucosa. The associations between miR-23a and miR-23b expression, and the selected clinicopathological characteristics and patients' prognosis were also evaluated.

Results: MiR-23a (GC vs. Normal: 3.98 ± 1.23 vs. 2.29 ± 1.12, P < 0.001) and miR-23b (GC vs. Normal: 3.70 ± 1.24 vs. 1.58 ± 1.18, P < 0.001) expression were both increased dramatically when compared with paired normal mucosa. Notably, the expression levels of miR-23a in GC tissues were positively correlated with those of miR-23b (Spearman correlation coefficient r = 0.77, P < 0.001). Then, the coexpression of miR-23a and miR-23b (miR-23a-high/miR-23b-high) in GC tissues was significantly associated with the advanced TNM stage (P < 0.001), the presence of lymph node metastasis (P = 0.008) and the great depth of invasion (P = 0.02). Furthermore, both univariate and multivariate analyses showed that miR-23a/miR-23b co-expression was an independent predictor for unfavorable overall survival.

Conclusions: These results suggest that the dysregulation of miR-23a and miR-23b may be implicated in the progression of human GC. Combined expression of miR-23a and miR-23b appears to be a valuable marker for prognosis of this disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314044PMC
October 2015

Overexpression of zinc finger E-box binding homeobox factor 1 promotes tumor invasiveness and confers unfavorable prognosis in esophageal squamous cell carcinoma.

Tumour Biol 2014 Dec 21;35(12):11977-84. Epub 2014 Aug 21.

Department of Gastrogenterology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, 223300, Jiangsu, China.

Zinc finger E-box binding homeobox factor 1 (ZEB1), as a crucial mediator of "epithelial-mesenchymal transition," contributes to malignant progression of various epithelial tumors. However, its involvement in human esophageal squamous cell carcinoma (ESCC) remains unclear. In order to investigate the expression pattern of ZEB1 in ESCC tissues and evaluate its associations with tumor progression and patients' prognosis, 100 pairs of formalin-fixed and paraffin-embedded cancerous and adjacent noncancerous tissues from patients with ESCC were used to detect the expression pattern of ZEB1 by immunohistochemistry. Then, the association between ZEB1 expression, clinicopathological parameters, and prognosis of ESCC was examined. We also performed migration and invasion assays of small interfering RNA (siRNA)-targeted ZEB1-transfected cells in vitro. As a result, expression level of ZEB1 was significantly higher in ESCC tissues compared to that in adjacent noncancerous tissues (P < 0.001). High expression of ZEB1 was observed in 55.00 % (55/100) of ESCCs. In addition, high ZEB1 expression was found to be closely correlated with advanced tumor stage (P = 0.001), positive lymph node metastasis (P = 0.001), great tumor depth (P = 0.03), and high histologic grade (P = 0.008). Moreover, multivariate analysis showed that the status of ZEB1 expression was an independent predictor for overall survival in ESCC. Furthermore, knockdown of ZEB1 by transfection of siRNA-ZEB1 abrogated the migration and invasion of ESCC cells in vitro. Taken together, our data offer the convincing evidence that ZEB1 may play a crucial role in promoting aggressive ESCC progression. ZEB1 may serve as an effective prognostic marker and a potential target for therapeutic intervention of ESCC.
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http://dx.doi.org/10.1007/s13277-014-2494-8DOI Listing
December 2014

Pancreaticogastrostomy versus pancreaticojejunostomy.

J Surg Res 2014 Nov 15;192(1):68-75. Epub 2014 May 15.

Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, P. R. China. Electronic address:

Background: It has long been debated whether pancreaticogastrostomy (PG) or pancreaticojejunostomy (PJ) is the better choice for reconstruction after pancreaticoduodenectomy. The purpose of this study is to evaluate the two techniques.

Methods: Randomized controlled trials (RCTs) comparing PG with PJ published from January 1995 to January 2014 were searched electronically using PubMed, Medline, and Cochrane Library. Published data of these RCTs were analyzed using either fixed-effects model or random-effects model.

Results: Seven RCTs were included in this meta-analysis, with a total of 1121 patients (562 in PG, 559 in PJ). The incidence of postoperative pancreatic fistula and intra-abdominal fluid collection were significantly lower in PG than in PJ (respectively: odds ratio = 0.53 [0.37, 0.74], P < 0.001; odds ratio = 0.48 [0.30, 0.76], P < 0.01), no significant difference could be found for delayed gastric emptying, hemorrhage, morbidity, reoperation rate, and mortality.

Conclusions: The evidence from RCTs suggests that PG technique is associated with a lower rate of postoperative pancreatic fistula and intra-abdominal fluid collection than PJ.
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http://dx.doi.org/10.1016/j.jss.2014.05.015DOI Listing
November 2014

Soy Sauce Residue Oil Extracted by a Novel Continuous Phase Transition Extraction under Low Temperature and Its Refining Process.

J Agric Food Chem 2014 Apr 28;62(14):3230-3235. Epub 2014 Mar 28.

College of Food Science and ‡Instrumental Analysis & Reasearch Center, South China Agricultural University , NO. 483 Wushan Road, Guangzhou 510642, People's Republic of China.

On the basis of previous single-factor experiments, extraction parameters of soy sauce residue (SSR) oil extracted using a self-developed continuous phase transition extraction method at low temperature was optimized using the response surface methodology. The established optimal conditions for maximum oil yield were n-butane solvent, 0.5 MPa extraction pressure, 45 °C temperature, 62 min extraction time, and 45 mesh raw material granularity. Under these conditions, the actual yield was 28.43% ± 0.17%, which is relatively close to the predicted yield. Meanwhile, isoflavone was extracted from defatted SSR using the same method, but the parameters and solvent used were altered. The new solvent was 95% (v/v) ethanol, and extraction was performed under 1.0 MPa at 60 °C for 90 min. The extracted isoflavones, with 0.18% ± 0.012% yield, mainly comprised daidzein and genistein, two kinds of aglycones. The novel continuous phase transition extraction under low temperature could provide favorable conditions for the extraction of nonpolar or strongly polar substances. The oil physicochemical properties and fatty acids compositions were analyzed. Results showed that the main drawback of the crude oil was the excess of acid value (AV, 63.9 ± 0.1 mg KOH/g) and peroxide value (POV, 9.05 ± 0.3 mmol/kg), compared with that of normal soybean oil. However, through molecular distillation, AV and POV dropped to 1.78 ± 0.12 mg KOH/g and 5.9 ± 0.08 mmol/kg, respectively. This refined oil may be used as feedstuff oil.
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http://dx.doi.org/10.1021/jf405459vDOI Listing
April 2014

Template synthesis of three-dimensional cubic ordered mesoporous carbon with tunable pore sizes.

Nanoscale Res Lett 2009 Oct 14;5(1):103-7. Epub 2009 Oct 14.

Nanomaterials Research Institute, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, 210016, Nanjing, People's Republic of China.

Three-dimensional cubic ordered mesoporous carbons with tunable pore sizes have been synthesized by using cubic Ia3d mesoporous KIT-6 silica as the hard template and boric acid as the pore expanding agent. The prepared ordered mesoporous carbons were characterized by powder X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and nitrogen adsorption-desorption analysis. The results show that the pore sizes of the prepared ordered mesoporous carbons with three-dimensional cubic structure can be regulated in the range of 3.9-9.4 nm. A simplified model was proposed to analyze the tailored pore sizes of the prepared ordered mesoporous carbons on the basis of the structural parameters of the silica template.
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http://dx.doi.org/10.1007/s11671-009-9450-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894178PMC
October 2009

Structural characterization of mesoporous silica nanofibers synthesized within porous alumina membranes.

Nanoscale Res Lett 2009 Jul 14;4(11):1257-62. Epub 2009 Jul 14.

Nanomaterials Research Institute, College of Material Science and Technology, Nanjing University of Aeronautics and Astronautics, 210016, Nanjing, People's Republic of China.

Mesoporous silica nanofibers were synthesized within the pores of the anodic aluminum oxide template using a simple sol-gel method. Transmission electron microscopy investigation indicated that the concentration of the structure-directing agent (EO20PO70EO20) had a significant impact on the mesostructure of mesoporous silica nanofibers. Samples with alignment of nanochannels along the axis of mesoporous silica nanofibers could be formed under the P123 concentration of 0.15 mg/mL. When the P123 concentration increased to 0.3 mg/mL, samples with a circular lamellar mesostructure could be obtained. The mechanism for the effect of the P123 concentration on the mesostructure of mesoporous silica nanofibres was proposed and discussed.
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http://dx.doi.org/10.1007/s11671-009-9389-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894318PMC
July 2009
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