Publications by authors named "Weijia Wang"

179 Publications

Epidemiological and clinical characteristics of respiratory viruses in 4403 pediatric patients from multiple hospitals in Guangdong, China.

BMC Pediatr 2021 Jun 17;21(1):284. Epub 2021 Jun 17.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Acute respiratory infections (ARI) cause considerable morbidity and mortality worldwide, especially in children. Unfortunately, there are limited multi-center data on common viral respiratory infections in south China.

Methods: A total of 4403 nasal swabs were collected from children in 10 cities in Guangdong, China in 2019. Seven respiratory viruses, influenza A virus (IFA), influenza B virus (IFB), respiratory syncytial virus (RSV), adenoviruses (ADV) and parainfluenza virus types 1-3 (PIV1, PIV2 and PIV3), were detected by direct immunofluorescence antibody assay. The personal information and clinical characteristics were recorded and analyzed.

Results: The results showed that at least one virus was detected in 1099 (24.96 %) samples. The detection rates of RSV, IFA, ADV, PIV3, PIV1 and PIV2 were 7.13 % (314/4403), 5.31 % (234/4403), 4.02 % (177/4403), 3.04 % (134/4403), 1.70 % (75/4403) and 1.16 % (51/4403), respectively. The detection rate of RSV was highest in 0-6-month-old children at 18.18 % (106/583), while the detection rate of IFA was highest in 12-18-year-old children at 20.48 % (17/83). The total detection rates in winter and spring were 35.67 % (219/614) and 34.56 % (403/1166), higher than those in summer, 17.41 % (284/1631), and autumn, 19.46 % (193/992).

Conclusions: RSV and IFA were the main respiratory viruses in children. With increasing age the detection rate of RSV decreased in children, but the trends for the detection rates of IFA and IFB were the opposite. This study provided the viral etiology and epidemiology of pediatric patients with ARI in Guangdong, China.
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http://dx.doi.org/10.1186/s12887-021-02759-0DOI Listing
June 2021

Identifying Periampullary Regions in MRI Images Using Deep Learning.

Front Oncol 2021 28;11:674579. Epub 2021 May 28.

Department of General Surgery (Hepatobiliary Surgery), The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Background: Development and validation of a deep learning method to automatically segment the peri-ampullary (PA) region in magnetic resonance imaging (MRI) images.

Methods: A group of patients with or without periampullary carcinoma (PAC) was included. The PA regions were manually annotated in MRI images by experts. Patients were randomly divided into one training set, one validation set, and one test set. Deep learning methods were developed to automatically segment the PA region in MRI images. The segmentation performance of the methods was compared in the validation set. The model with the highest intersection over union (IoU) was evaluated in the test set.

Results: The deep learning algorithm achieved optimal accuracies in the segmentation of the PA regions in both T1 and T2 MRI images. The value of the IoU was 0.68, 0.68, and 0.64 for T1, T2, and combination of T1 and T2 images, respectively.

Conclusions: Deep learning algorithm is promising with accuracies of concordance with manual human assessment in segmentation of the PA region in MRI images. This automated non-invasive method helps clinicians to identify and locate the PA region using preoperative MRI scanning.
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http://dx.doi.org/10.3389/fonc.2021.674579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193851PMC
May 2021

Enhanced photocatalytic hydrogen production activity of Janus CuS-ZnS spherical nanoheterostructures.

J Colloid Interface Sci 2021 May 18;600:838-846. Epub 2021 May 18.

State Key Laboratory for Powder Metallurgy, Powder Metallurgy Research Institute, Central South University, Changsha 410083, China; Research Institute of Resource Recycling, Central South University, Changsha 410083, China.

Photocatalytic hydrogen evolution is one of the most promising approaches for efficient solar energy conversion. The light-harvesting ability and interfacial structure of heterostructured catalysts regulate the processes of photon injection and transfer, which further determines their photocatalytic performances. Here, we report a Janus CuS-ZnS nano-heterostructured photocatalyst synthesized using a facile stoichiometrically limited cation exchange reaction. Djurleite CuS and wurtzite ZnS share the anion skeleton, and the lattice mismatch between immiscible domains is ∼1.7%. Attributing to the high-quality interfacial structure, Janus CuS-ZnS nanoheterostructures (NHs) show an enhanced photocatalytic hydrogen evolution rate of up to 0.918 mmol h g under full-spectrum irradiation, which is ∼38-fold and 17-fold more than those of sole CuS and ZnS nanocrystals (NCs), respectively. The results indicate that cation exchange reaction is an efficient approach to construct well-ordered interfaces in hybrid photocatalysts, and it also demonstrates that reducing lattice mismatch and interfacial defects in hybrid photocatalysts is essential for enhancing their solar energy conversion performance.
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http://dx.doi.org/10.1016/j.jcis.2021.05.073DOI Listing
May 2021

Cytokine combinations for human blood stem cell expansion induce cell type- and cytokine-specific signaling dynamics.

Blood 2021 May 14. Epub 2021 May 14.

University Hospital Tubingen, Germany.

How hematopoietic stem cells (HSCs) integrate signals from their environment to make fate decisions remains incompletely understood. Current knowledge is based on either averages of heterogeneous populations or snapshot analyses, both missing important information about the dynamics of intracellular signaling activity. By combining fluorescent biosensors with time-lapse imaging and microfluidics, we measured the activity of the extracellular signal-regulated kinase (ERK) pathway over time (i.e. dynamics) in live single human umbilical cord blood HSCs and multipotent progenitor cells (MPPs). In single cells, ERK signaling dynamics were highly heterogeneous and depended on the cytokines, their combinations, and cell types. ERK signaling was activated by SCF and FLT3L in HSCs, but by SCF, IL3 and GCSF in MPPs. Different cytokines and their combinations led to distinct ERK signaling dynamics frequencies, and ERK dynamics in HSCs were more transient than those in MPPs. A combination of 5 cytokines recently shown to maintain HSCs in long-term culture, had a more-than-additive effect in eliciting sustained ERK dynamics in HSCs. ERK signaling dynamics also predicted future cell fates. E.g. CD45RA expression increased more in HSC daughters with intermediate than with transient or sustained ERK signaling. We demonstrate heterogeneous, cytokine- and cell type- specific ERK signaling dynamics, illustrating their relevance in regulating HSPC fates.
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http://dx.doi.org/10.1182/blood.2020008386DOI Listing
May 2021

Economic Analysis on Adoption of Biosimilar Granulocyte Colony-Stimulating Factors in Patients With Nonmyeloid Cancer at Risk of Febrile Neutropenia Within the Oncology Care Model Framework.

JCO Oncol Pract 2021 May 7:OP2000994. Epub 2021 May 7.

Scientia Pharmacy Advisors, Boston, MA.

Purpose: Value-based programs, such as the Oncology Care Model (OCM), seek to improve care for patients undergoing chemotherapy, while reducing total costs. The purpose of this study is to quantify the impact of adopting biosimilar granulocyte colony-stimulating factors (G-CSFs) for febrile neutropenia (FN) primary prophylaxis (PP) from a US practice perspective.

Methods: A 1-year economic analysis on real-world direct drug costs and health care resource utilization was conducted in a hypothetical cohort of 500 patients with nonmyeloid cancer receiving chemotherapy. The first model simulated total cost savings of biosimilar versus reference G-CSFs over six cycles of chemotherapy. The second model evaluated cost and outcome implications of expanding the use of biosimilar G-CSFs to an additional 10% of patients at intermediate FN risk.

Results: Based on real-world evidence over 1 year, a total of 121 of 500 patients received G-CSF prophylaxis resulting in cost savings that ranged from $0.54M US dollars (USD) (short-acting, eg, filgrastim) to $1.68M USD (long-acting, eg, pegfilgrastim) when switching from reference to biosimilar G-CSFs. Expanding the use of biosimilar G-CSFs allowed an additional 24 patients to receive prophylaxis of FN, leading to cost savings of $0.03M USD or $1.19M USD, with a reduction of $0.08M USD in FN-related resource utilization cost. The per-patient per-year cost saving for long-acting G-CSFs was about $3,000 USD.

Conclusion: The implementation of biosimilar versus reference G-CSFs to OCM-participating practices results in a reduction of costs and facilitates achieving OCM metrics by improving patients' outcomes while expanding biosimilar G-CSFs access to patients at intermediate risk of chemotherapy-induced FN.
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http://dx.doi.org/10.1200/OP.20.00994DOI Listing
May 2021

Prevalence of Frailty and Associations with Oral Anticoagulant Prescribing in Atrial Fibrillation.

J Gen Intern Med 2021 May 4. Epub 2021 May 4.

Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA, USA.

Background: Frailty is often cited as a factor influencing oral anticoagulation (OAC) prescription in patients with non-valvular atrial fibrillation (NVAF). We sought to determine the prevalence of frailty and its association with OAC prescription in older veterans with NVAF.

Methods: We used ICD-9 codes in Veterans Affairs (VA) records and Medicare claims data to identify patients with NVAF and CHADSVASC ≥2 receiving care between February 2010 and September 2015. We examined rates of OAC prescription, further stratified by direct oral anticoagulant (DOAC) or vitamin K antagonist (VKA). Participants were characterized into 3 categories: non-frail, pre-frail, and frail based on a validated 30-item EHR-derived frailty index. We examined relations between frailty and OAC receipt; and frailty and type of OAC prescribed in regression models adjusted for factors related to OAC prescription.

Results: Of 308,664 veterans with NVAF and a CHADSVASC score ≥2, 121,839 (39%) were prescribed OAC (73% VKA). The mean age was 77.7 (9.6) years; CHADSVASC and ATRIA scores were 4.6 (1.6) and 5.0 (2.9) respectively. Approximately a third (38%) were frail, another third (32%) were pre-frail, and the remainder were not frail. Veterans prescribed OAC were younger, had higher bleeding risk, and were less likely to be frail than participants not receiving OAC (all p's<0.001). After adjustment for factors associated with OAC use, pre-frail (OR: 0.89, 95% CI: 0.87-0.91) and frail (OR: 0.66, 95% CI: 0.64-0.68) veterans were significantly less likely to be prescribed OAC than non-frail veterans. Of those prescribed OAC, pre-frail (OR:1.27, 95% CI: 1.22-1.31) and frail (OR: 1.75, 95% CI: 1.67-1.83) veterans were significantly more likely than non-frail veterans to be prescribed a DOAC than a VKA.

Conclusions: There are high rates of frailty among older veterans with NVAF. Frailty using an EHR-derived index is associated with decreased OAC prescription.
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http://dx.doi.org/10.1007/s11606-021-06834-1DOI Listing
May 2021

Economic and clinical outcomes of pegfilgrastim via prefilled syringe vs on-body injector: a real-world data analysis.

J Manag Care Spec Pharm 2021 Apr 30:1-9. Epub 2021 Apr 30.

Sandoz, Inc, Princeton, NJ.

Pegfilgrastim is available as a prefilled syringe (PFS) and an on-body injector (OBI). Whether the administration method of pegfilgrastim affects the effectiveness and health care resources has not been evaluated in the setting of routine care. To compare real-world clinical and economic outcomes between PFS and OBI methods of administration. This was a retrospective observational study in patients diagnosed with breast cancer or non-Hodgkin lymphoma who received myelosuppressive chemotherapy and prophylactic use of pegfilgrastim via PFS or OBI between January 1, 2017, and May 31, 2018, according to MarketScan research databases. A propensity score was used to match the PFS cohort 1:1 to the OBI cohort. Outcomes were compared among the matched cohorts using a generalized linear model and generalized estimating equations with log-link function. 3,152 patients were identified. After matching, the final sample included 2,170 patients, representing 1,085 in each cohort. The incidence of febrile neutropenia (FN) in the first chemotherapy cycle was 1.01% for OBI (95% CI = 0.56-1.82) vs 1.48% for PFS (95% CI = 0.91-2.39; = 0.336). In all chemotherapy cycles (total cycles = 7,467), the FN incidence was 0.91% for OBI (95% CI = 0.64-1.30) vs 1.22% for PFS (95% CI = 0.90-1.64; = 0.214). There was no statistically significant difference in adjusted per-member per-month all-cause total cost health care resource utilization (HCRU) for hospitalizations, emergency department visits, and pharmacy claims. In a matched cohort of patients representing real-world utilization, there was no statistically or clinically meaningful difference in FN incidence between OBI and PFS methods of pegfilgrastim administration. There was no difference in total HCRU or total costs. OBI and PFS methods of administration are both indicated for patients requiring prophylactic pegfilgrastim, which is important considering that biosimilar PFS options are now available. This study was funded by Sandoz, Inc. Wang, Li, and K. Campbell are employees of Sandoz, Inc. Schroader and D. Campbell are employees of Xcenda, which was contracted by Sandoz, Inc., to provide study and manuscript development. McBride reports receiving payment from Sandoz, Inc., as a consultant, unrelated to this study; Coherus for advisory board and speaker engagements; and Pfizer for advisory board participation during the time of this study.
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http://dx.doi.org/10.18553/jmcp.2021.21010DOI Listing
April 2021

Identification of key genes and pathways in discoid lupus skin via bioinformatics analysis.

Medicine (Baltimore) 2021 Apr;100(16):e25433

Department of Laboratory Medicine, Zhongshan Hospital of Sun Yat-sen University, Zhongshan, Guangdong, China.

Abstract: Discoid lupus erythematosus (DLE) is the most common skin manifestation of lupus; however, the molecular mechanisms underlying DLE remain unknown. Therefore, we aimed to identify key differentially expressed genes (DEGs) in discoid lupus skin and investigate their potential pathways.To identify candidate genes involved in the occurrence and development of the disease, we downloaded the microarray datasets GSE52471 and GSE72535 from the Gene Expression Database (GEO). DEGs between discoid lupus skin and normal controls were selected using the GEO2R tool and Venn diagram software (http://bioinformatics.psb.ugent.be/webtools/Venn/). The Database for Annotation, Visualization, and Integrated Discovery (DAVID), Enrichr, and Cytoscape ClueGo were used to analyze the Kyoto Encyclopedia of Gene and Genome pathways and gene ontology. Protein-protein interactions (PPIs) of these DEGs were further assessed using the Search Tool for the Retrieval Interacting Genes version 10.0.Seventy three DEGs were co-expressed in both datasets. DEGs were predominantly upregulated in receptor signaling pathways of the immune response. In the PPI network, 69 upregulated genes were selected. Furthermore, 4 genes (CXCL10, ISG15, IFIH1, and IRF7) were found to be significantly upregulated in the RIG-I-like receptor signaling pathway, from analysis of Enrichr and Cytoscape ClueGo.The results of this study may provide new insights into the potential molecular mechanisms of DLE. However, further experimentation is required to confirm these findings.
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http://dx.doi.org/10.1097/MD.0000000000025433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078291PMC
April 2021

Resveratrol Derivative, Trans-3, 5, 4'-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation.

Oxid Med Cell Longev 2021 25;2021:8840692. Epub 2021 Mar 25.

Institute of Advanced Diagnostic and Clinical Medicine, Zhongshan People's Hospital, Guangzhou University & Zhongshan People's Hospital Joint Biomedical Institute, 2 Sun Wen East Road, Zhongshan, Guangdong 528400, China.

Numerous studies have shown that resveratrol can induce apoptosis in cancer cells. Trans-3, 5, 4'-trimethoxystilbene (TMS), a novel derivative of resveratrol, is a more potent anticancer compound than resveratrol and can induce apoptosis in cancer cells. Herein, we examined the mechanisms involved in TMS-mediated sensitization of human osteosarcoma (143B) cells to TNF-related apoptosis-inducing ligand- (TRAIL-) induced apoptosis. Our results showed that cotreatment with TSM and TRAIL activated caspases and increased PARP-1 cleavage in 143B cells. Decreasing cellular ROS levels using NAC reversed TSM- and TRAIL-induced apoptosis in 143B cells. NAC abolished the upregulated expression of PUMA and p53 induced by treatment with TRAIL and TSM. Silencing the expression of p53 or PUMA using RNA interference attenuated TSM-mediated sensitization of 143B cells to TRAIL-induced apoptosis. Knockdown of Bax also reversed TSM-induced sensitization of 143B cell to TRAIL-mediated apoptotic cell death. These results indicate that cotreatment with TRAIL and TSM evaluated intracellular ROS level, promoted DNA damage, and activated the Bax/PUMA/p53 pathway, leading to activation of both mitochondrial and caspase-mediated apoptosis in 143B cells. Orthotopic implantation of 143B cells in mice also demonstrated that cotreatment with TRAIL and TSM reversed resistance to apoptosis in cells without obvious adverse effects in normal cells.
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http://dx.doi.org/10.1155/2021/8840692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018847PMC
May 2021

Prognostic value of geriatric conditions for death and bleeding in older patients with atrial fibrillation.

Int J Cardiol Heart Vasc 2021 Apr 4;33:100739. Epub 2021 Mar 4.

Cardiology Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.

Background: Geriatric conditions, such as frailty and cognitive impairment, are prevalent in older patients with atrial fibrillation (AF). We examined the prognostic value of geriatric conditions for predicting 1-year mortality and bleeding events in these patients.

Methods: SAGE (Systematic Assessment of Geriatric Elements)-AF study is a multicenter cohort study which enrolled individuals (mean age 75 years, 48% women, 86% taking oral anticoagulation) 65 years and older with AF and CHADS -VASc score of 2 or higher from clinics in Massachusetts and Georgia, USA between 2016 and 2018. A six-component geriatric assessment included validated measures of frailty, cognitive function, social support, depressive symptoms, vision, and hearing was performed at baseline. Study endpoints included all-cause mortality and clinically relevant bleeding.

Results: At 1 year, 1,097 (96.5%) individuals attended the follow up visit, 44 (3.9%) had died, and 56 (5.1%) had clinically relevant bleeding. After adjustment for demographic and clinical factors, social isolation (odds ratio [OR] 1.69, 95% confidence interval [CI]: 1.01-2.84), depression (OR 1.94, 95% CI: 1.28-2.95) and frailty (OR 2.55, 95% CI: 1.55-4.19) were significantly associated with the composite endpoint of death or clinically relevant bleeding. After multivariable adjustment, depression (OR 1.79, 95% CI 1.09-2.93) and frailty (OR 2.83, 95% CI 1.55-5.17) were significantly associated with clinically relevant bleeding.

Conclusions:  Social isolation, depression, and frailty were prognostic of dying or experiencing clinically relevant bleeding during the coming year in older men and women with AF. Assessing geriatric impairments merits consideration in the care of these patients.
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http://dx.doi.org/10.1016/j.ijcha.2021.100739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935705PMC
April 2021

Identification of an Immune-Related Signature for Predicting Prognosis in Patients With Pancreatic Ductal Adenocarcinoma.

Front Oncol 2020 24;10:618215. Epub 2021 Feb 24.

Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Department of Hepato-Pancreato-Biliary Surgery, Peking University Cancer Hospital & Institute, Beijing, China.

Purpose: Pancreatic ductal adenocarcinoma (PDAC) is one of the highest fatality rate cancers with poor survival rates. The tumor microenvironment (TME) is vital for tumor immune responses, leading to resistance to chemotherapy and poor prognosis of PDAC patients. This study aimed to provide a comprehensive evaluation of the immune genes and microenvironment in PDAC that might help in predicting prognosis and guiding clinical treatments.

Methods: We developed a prognosis-associated immune signature (i.e., PAIS) based on immune-associated genes to predict the overall survival of patients with PDAC. The clinical significance and immune landscapes of the signature were comprehensively analyzed.

Results: Owing to gene expression profiles from TCGA database, functional enrichment analysis revealed a significant difference in the immune response between PDAC and normal pancreas. Using transcriptome data analysis of a training set, we identified an immune signature represented by 5 genes (ESR2, IDO1, IL20RB, PPP3CA, and PLAU) related to the overall survival of patients with PDAC, significantly. This training set was well-validated in a test set. Our results indicated a clear association between a high-risk score and a very poor prognosis. Stratification analysis and multivariate Cox regression analysis revealed that PAIS was an important prognostic factor. We also found that the risk score was positively correlated with the inflammatory response, antigen-presenting process, and expression level of some immunosuppressive checkpoint molecules (e.g., CD73, PD-L1, CD80, and B7-H3). These results suggested that high-risk patients had a suppressed immune response. However, they could respond better to chemotherapy. In addition, PAIS was positively correlated with the infiltration of M2 macrophages in PDAC.

Conclusions: This study highlighted the relationship between the immune response and prognosis in PDAC and developed a clinically feasible signature that might serve as a powerful prognostic tool and help further optimize the cancer therapy paradigm.
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http://dx.doi.org/10.3389/fonc.2020.618215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945593PMC
February 2021

Profiling of RNA -Methyladenosine Methylation Reveals the Critical Role of mA in Chicken Adipose Deposition.

Front Cell Dev Biol 2021 5;9:590468. Epub 2021 Feb 5.

Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China.

One of the main objectives of broiler breeding is to prevent excessive abdominal adipose deposition. The role of RNA modification in adipose deposition is not clear. This study was aimed to map mA modification landscape in chicken adipose tissue. MeRIP-seq was performed to compare the differences in mA methylation pattern between fat and lean broilers. We found that start codons, stop codons, coding regions, and 3'-untranslated regions were generally enriched for mA peaks. The high mA methylated genes (fat birds vs. lean birds) were primarily associated with fatty acid biosynthesis and fatty acid metabolism, while the low mA methylated genes were mainly involved in processes associated with development. Furthermore, we found that the mRNA levels of many genes may be regulated by mA modification. This is the first comprehensive characterization of mA patterns in the chicken adipose transcriptome, and provides a basis for studying the role of mA modification in fat deposition.
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http://dx.doi.org/10.3389/fcell.2021.590468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892974PMC
February 2021

CYP2C9 inhibits the invasion and migration of esophageal squamous cell carcinoma via downregulation of HDAC.

Mol Cell Biochem 2021 May 29;476(5):2011-2020. Epub 2021 Jan 29.

Department of Pharmacy, Hangzhou Cancer Hospital, Hangzhou, 310002, Zhejiang, China.

Cytochrome P450 2C9 (CYP2C9) is involved in the metabolism of cancer drugs and exogenous carcinogens. In our study, CYP2C9 was downregulated in multiple cohorts of human esophageal squamous cell carcinoma (ESCC). Until now, its role and epigenetic regulation of CYP2C9 repression in ESCC remain poorly understood. CYP2C9 repression in collected ESCC patient tumor tissues was demonstrated by RT-qPCR and Western blot. The histone acetylation level was carried out by the treatment of histone deacetylase inhibitor TSA and RNA interference. Epigenetic analysis revealed that the increased expression of CYP2C9 in KYSE-150 and TE1 cells was characterized by inhibition of HDAC8 and HDAC1, respectively. TSA decreased the levels of HDAC occupancy around CYP2C9 promoter region greatly. Overexpression of CYP2C9 reduced the invasion and migration of ESCC cells.
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http://dx.doi.org/10.1007/s11010-021-04050-3DOI Listing
May 2021

Sphingosine-1-phosphate receptor 3 potentiates inflammatory programs in normal and leukemia stem cells to promote differentiation.

Blood Cancer Discov 2021 Jan 1;2(1):32-53. Epub 2020 Dec 1.

Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.

Acute myeloid leukemia (AML) is a caricature of normal hematopoiesis, driven from leukemia stem cells (LSC) that share some hematopoietic stem cell (HSC) programs including responsiveness to inflammatory signaling. Although inflammation dysregulates mature myeloid cells and influences stemness programs and lineage determination in HSC by activating stress myelopoiesis, such roles in LSC are poorly understood. Here, we show that S1PR3, a receptor for the bioactive lipid sphingosine-1-phosphate, is a central regulator which drives myeloid differentiation and activates inflammatory programs in both HSC and LSC. S1PR3-mediated inflammatory signatures varied in a continuum from primitive to mature myeloid states across AML patient cohorts, each with distinct phenotypic and clinical properties. S1PR3 was high in LSC and blasts of mature myeloid samples with linkages to chemosensitivity, while S1PR3 activation in primitive samples promoted LSC differentiation leading to eradication. Our studies open new avenues for therapeutic target identification specific for each AML subset.
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http://dx.doi.org/10.1158/2643-3230.BCD-20-0155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7116590PMC
January 2021

Long Non-Coding RNA ZNF667-AS1 Knockdown Curbs Liver Metastasis in Acute Myeloid Leukemia by Regulating the microRNA-206/AKAP13 Axis.

Cancer Manag Res 2020 23;12:13285-13300. Epub 2020 Dec 23.

Laboratory Diagnosis Center, Zhongshan People's Hospital, Zhongshan, 528403 Guangdong, People's Republic of China.

Background: Zinc finger protein 667-antisense RNA 1 (), a long non-coding RNA (lncRNA), plays important parts in tumorigenesis and development of esophageal squamous cell carcinoma, but its function in acute myeloid leukemia (AML) is unknown. Our goal here was to probe the functional mechanism of in AML by mediating microRNA-206 ()/A-kinase anchoring protein 13 () axis.

Materials And Methods: The bone marrow samples from AML patients and controls were selected for microarray analysis to select significantly upregulated lncRNAs. Next, effects of on cell aggressiveness of AML were assessed after delivery of cells with siRNA against . Subcellular fractionation location assay and FISH experiments were used to determine localization in cells. Dual-luciferase experiments detect the targeting relationships among , and . Finally, tumor growth and metastasis were evaluated in vivo to determine the relevance of / axis.

Results: The expression of was upregulated in AML patients, which predicted poor prognosis. Downregulation of reduced cell proliferation, invasion, tumorigenesis and metastasis. 6 inhibitor reversed the repressive role of knockdown in cell proliferation, invasion and tumorigenesis, while silencing flattened the stimulative role of inhibitor in AML malignant aggressiveness. Mechanistically, we demonstrated that functioned as a molecular sponge for . In addition, we observed that Wnt/β-catenin pathway was suppressed by knockdown.

Conclusion: potentiated AML progression by targeting the / axis. This indicates inhibition may act as a prospective therapeutic option for the treatment of AML.
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http://dx.doi.org/10.2147/CMAR.S269258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767707PMC
December 2020

Polymorphisms of KLF3 gene coding region and identification of their functionality for abdominal fat in chickens.

Vet Med Sci 2021 May 27;7(3):792-799. Epub 2020 Dec 27.

Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China.

KLF3 is a member of the Kruppel-like factor (KLF) family of transcription factors, and plays an important role in several biological processes, including adipogenesis, erythropoiesis and B-cell development. The purposes of this study are to search for polymorphisms of KLF3 coding region and to provide functional evidence for abdominal fat in chickens. A total of 168 SNPs in KLF3 coding region were detected in a unique chicken population, the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). Of which three single nucleotide polymorphisms (g.3452T > C, g.8663A > G and g.10751G > A) were significantly correlated with abdominal fat weight (AFW) and abdominal fat percentage (AFP) of 329 birds from the 19th generation of NEAUHLF (FDR < 0.05). The reporter gene assay was performed to verify functionality of these three SNPs in both ICP-1 and DF1 cells. Results showed that the luciferase activity of G allele was significantly higher than that of A allele in g.10751G > A (p < 0.05). However, there were no significant differences between different alleles of others two SNPs in luciferase activity. Overall, KLF3 is an important candidate gene that affects chicken abdominal fat content, and the g.10751G > A is a functional variant that potential would be applied to marker-assisted selection (MAS) for selective breeding programme.
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http://dx.doi.org/10.1002/vms3.422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136968PMC
May 2021

Development and Validation of a Novel Nomogram for Predicting Tumor-Distant-Metastasis in Patients with Early T1-2 Stage Lung Adenocarcinoma.

Ther Clin Risk Manag 2020 10;16:1213-1225. Epub 2020 Dec 10.

Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People's Republic of China.

Background: Distant metastasis in early T1-2 (diameter≤5 cm) stage lung adenocarcinoma (ET-LUAD) patients largely affect treatment strategies in clinical practice. However, the associated mechanism remains unclear and related studies is less. This study aimed to establish and validate a novel nomogram to predict the risk of distant metastasis in ET-LUAD.

Methods: A total of 258 patients diagnosed with ET-LUAD and not receiving any treatment were recruited into this study. The patients were randomly divided into a training cohort and validation cohort in a ratio of 1:2. Univariate and multivariate logistic regression analysis was used to select the most significant predictive risk factors associated with distant metastasis in the training cohort. The established nomogram was validated by the consistency index (C-index), calibration curve, and decision curve analysis (DCA).

Results: There were 124 patients with confirmed distant metastasis and 134 patients with non-distant metastases ET-LUAD were enrolled in the study. Multivariate logistic hazards regression analysis identified independent risk factors associated with distant metastasis to include platelet-to-lymphocyte ratios (PLR), lactate dehydrogenase (LDH), neural-specific enolase (NSE), carcinoembryonic antigen (CEA) and cytokeratin 19 fragments (Cyfra211), which were included in the establishment of the nomogram. The nomogram achieved a high consistency (C-index=0.792), good calibration, and high clinical application value in the validation cohort.

Conclusion: The established nomogram can be used to predict distant metastasis in high-risk ET-LUAD nonmetastasis patients and can also be used by doctors to guide preventive and individualized treatment for ET-LUAD patients.
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http://dx.doi.org/10.2147/TCRM.S272748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735943PMC
December 2020

Sudden Cardiac Death Prediction in Arrhythmogenic Right Ventricular Cardiomyopathy: A Multinational Collaboration.

Circ Arrhythm Electrophysiol 2021 01 9;14(1):e008509. Epub 2020 Dec 9.

Department of Medicine, Division of Cardiology (J.C.-T., W.W., A.B., C.T., B.M., S.C., J.E.C., D.P.J., H.T., H.C., C.A.J.), Johns Hopkins Hospital, Baltimore, MD.

Background: Arrhythmogenic right ventricular cardiomyopathy (ARVC) is associated with ventricular arrhythmias (VA) and sudden cardiac death (SCD). A model was recently developed to predict incident sustained VA in patients with ARVC. However, since this outcome may overestimate the risk for SCD, we aimed to specifically predict life-threatening VA (LTVA) as a closer surrogate for SCD.

Methods: We assembled a retrospective cohort of definite ARVC cases from 15 centers in North America and Europe. Association of 8 prespecified clinical predictors with LTVA (SCD, aborted SCD, sustained, or implantable cardioverter-defibrillator treated ventricular tachycardia >250 beats per minute) in follow-up was assessed by Cox regression with backward selection. Candidate variables included age, sex, prior sustained VA (≥30s, hemodynamically unstable, or implantable cardioverter-defibrillator treated ventricular tachycardia; or aborted SCD), syncope, 24-hour premature ventricular complexes count, the number of anterior and inferior leads with T-wave inversion, left and right ventricular ejection fraction. The resulting model was internally validated using bootstrapping.

Results: A total of 864 patients with definite ARVC (40±16 years; 53% male) were included. Over 5.75 years (interquartile range, 2.77-10.58) of follow-up, 93 (10.8%) patients experienced LTVA including 15 with SCD/aborted SCD (1.7%). Of the 8 prespecified clinical predictors, only 4 (younger age, male sex, premature ventricular complex count, and number of leads with T-wave inversion) were associated with LTVA. Notably, prior sustained VA did not predict subsequent LTVA (=0.850). A model including only these 4 predictors had an optimism-corrected C-index of 0.74 (95% CI, 0.69-0.80) and calibration slope of 0.95 (95% CI, 0.94-0.98) indicating minimal over-optimism.

Conclusions: LTVA events in patients with ARVC can be predicted by a novel simple prediction model using only 4 clinical predictors. Prior sustained VA and the extent of functional heart disease are not associated with subsequent LTVA events.
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http://dx.doi.org/10.1161/CIRCEP.120.008509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834666PMC
January 2021

Economic modeling of reSET-O, a prescription digital therapeutic for patients with opioid use disorder.

J Med Econ 2021 Jan-Dec;24(1):61-68

Department of Population Health Sciences, Weill Cornell Medical College, New York, NY, USA.

Aims: reSET-O is a Food and Drug Administration-cleared prescription digital therapeutic (PDT) indicated to improve outpatient-treatment retention of patients with opioid use disorder (OUD). This study examined the cost-effectiveness and budget impact of reSET-O in conjunction with treatment as usual (reSET-O + TAU) relative to TAU.

Materials And Methods: Adult patients with ≥1 OUD diagnosis, treated with buprenorphine from 1 January 2015 to 30 March 2018, were identified from Truven Health MarketScan Commercial and Medicare Supplemental Research Databases. Twelve-week healthcare resource utilization (HCRU) costs for patients categorized as adherent and nonadherent to buprenorphine treatment were estimated. Total 12-week costs included OUD treatment and other HCRU costs. The cost-effectiveness of reSET-O + TAU was modeled in accordance with prior clinical trial outcomes. The 12-week budget impact of reSET-O was modeled for a 1 million-member healthcare plan.

Results: Higher buprenorphine adherence was associated with lower HCRU costs in claims data. Twelve-week per-patient total costs were $305 more for those receiving reSET-O + TAU than those receiving TAU. The incremental cost-effectiveness ratio was $18.70 per 1 percentage-point increase in the treatment retention rate. The probability that reSET-O + TAU would be considered cost-effective was over 92% for willingness-to-pay thresholds of $6,000 or more. The 12-week budget impact of reSET-O was $8,908, translating to $0.003 per member per month.

Limitations: The findings of the cost-effectiveness and budget impact modeling are limited by the assumptions of the models due to uncertainty around some inputs. While no model is free of bias, the inputs for this model were carefully selected to reflect contemporary treatment patterns.

Conclusions: Depending on the payer's willingness to pay, reSET-O may be cost-effective in increasing buprenorphine treatment retention rates. reSET-O results in an approximate budget impact of $0.003 per member per month, depending on market share and the prevalence of the population receiving treatment for OUD.
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http://dx.doi.org/10.1080/13696998.2020.1858581DOI Listing
December 2020

Wearable Bracelet Monitoring the Solar Ultraviolet Radiation for Skin Health Based on Hybrid IPN Hydrogels.

ACS Appl Mater Interfaces 2020 Dec 2;12(50):56480-56490. Epub 2020 Dec 2.

Key Laboratory of Advanced Textile Materials & Manufacturing Technology, Ministry of Education, Zhejiang Sci-Tech University, 310018 Hangzhou, China.

The risk of extensive exposure of the human epidermis to solar ultraviolet radiation is significantly increased nowadays. It not only induces skin aging and solar erythema but also increases the possibility of skin cancer. Therefore, a simply prepared, highly sensitive, and optically readable device for monitoring the solar ultraviolet radiation is highly desired for the skin health management. Because of the photoinitiated polymerization triggered by graphene-carbon nitride (g-CN) under ultraviolet radiation, g-CN is homogeneously distributed in the hybrid hydrogels containing -isopropylacrymide (NIPAM), poly(ethylene glycol) methyl ether methacrylate (OEGMA), and sodium alginate (SA). By further immersing the hybrid hydrogels into calcium chloride solution, hybrid alginate-Ca/P(NIPAM--OEGMA)/g-CN interpenetrating polymeric network (IPN) hydrogels are obtained. Due to the homogeneous distribution of g-CN and the existence of thermoresponsive polymers, the hybrid IPN hydrogels present good adsorption capability and high degradation efficiency for methylene blue (MB) especially at high temperature under ultraviolet radiation. Based on this unique property, the bracelet monitoring skin health is prepared by simply immersing the hybrid IPN hydrogels into the MB solution and then wrapping it with PET foil. Because the immersion time for the top, middle, and bottom parts of the hybrid IPN hydrogels is gradually increased, their colors vary from light to dark blue. A longer time is required for the discoloration of the darker part under solar ultraviolet radiation. Thus, the bracelet can be used to conveniently monitor the dose of solar ultraviolet radiation by simply checking the discoloration in the bracelet under sunshine. Due to the facile preparation and low cost of the bracelet, it is a promising candidate for wearable devices for skin health management.
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http://dx.doi.org/10.1021/acsami.0c17628DOI Listing
December 2020

Diagnosing acute promyelocytic leukemia by using convolutional neural network.

Clin Chim Acta 2021 Jan 4;512:1-6. Epub 2020 Nov 4.

Department of Laboratory Medicine, Zhongshan Hospital, Sun Yat-sen University, Zhongshan, PR China.

Purpose: To evaluate the efficacy of diagnosis systems based upon instance segmentation with convolutional neural networks (CNNs) for diagnosing acute promyelocytic leukemia (APL) in bone marrow smear images.

Materials And Methods: A self-established dataset was used in this study that was exempted from review by the institution review board, which consisted of 13,504 bone marrow smear images. One subset of the dataset with 12,215 labeled images was split into training (80%) and validation (20%), another with 1289 labeled images was used to test, in which each test entry consists of about 130 images. An instance segmentation method named Mask R-CNN was used to detect and classify the nucleated cells. Here, we train a trained neural network from scratch; for comparison, we also use a network pre-trained on MS COCO (common objects in context, a data set provided by Microsoft which can be used for image recognition, the images in MS coco dataset are divided into training, validation and test sets) and fine-tuned with our dataset and both were trained with same data augmentation scheme. Diagnosis systems based on trained models and "FAB Classification" (French-American-British classification systems, a series of diagnostic criteria for acute leukemia, which was first proposed in 1976) were developed for diagnosing the test entry as APL or as not. Average precision (AP) and average recall (AR) were used to evaluate model performance.

Results: The best-performing model had an average precision of 62.5%, which was the augmented pre-trained Mask R-CNN with average recall 84.1%. The average precision of the pre-trained model was greater than that of the model trained from scratch (P < 0.05). Augmenting the dataset further increased accuracy (P < 0 0.03).

Conclusion: Deep learning technology such as instance segmentation with Mask R-CNN may accurately diagnose APL in bone marrow smear images with an average precision of 62.5% when 0.5 as IoU thresholds. A data augmentation and pre-trained approach further improved accuracy.
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http://dx.doi.org/10.1016/j.cca.2020.10.039DOI Listing
January 2021

Fine particulate matter inhibits phagocytosis of macrophages by disturbing autophagy.

FASEB J 2020 12 30;34(12):16716-16735. Epub 2020 Oct 30.

Center for Translational Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, China.

Mounting evidence from epidemiological and clinical studies has revealed marked correlations between the air pollutant fine particulate matter (FPM) and respiratory diseases. FPM reaches distal airways and deposits in alveolar regions where it can act directly on alveolar macrophages. However, the detailed effect of FPM on the physiological function of alveolar macrophages and the underlying mechanisms remain unclear. In this study, we showed that exposing THP-1-derived macrophages to FPM led to autophagy dysfunction. FPM activated the extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway, which promoted the expression of autophagy-related 2A (ATG2A) and reactive oxygen species generation. The overexpression of ATG2A enhanced the synthesis of autophagic membranes, and the excessive production of reactive oxygen species caused autophagy flux inhibition through disrupting the lysosomal activity. More importantly, FPM impaired the phagocytic ability of macrophages on Escherichia coli and apoptotic neutrophils. Finally, we showed that restoring autophagy rescued the impairment of phagocytic ability induced by FPM. In summary, these results reveal the molecular mechanism of autophagy dysfunction caused by FPM and provide a novel approach to resolve the impaired function of macrophages in respiratory diseases induced by FPM.
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http://dx.doi.org/10.1096/fj.202000657RDOI Listing
December 2020

Root Crown Response to Fungal Root Rot in Middle American × Andean Lines.

Plant Dis 2020 Dec 20;104(12):3135-3142. Epub 2020 Oct 20.

Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824.

Fusarium root rot (FRR) is a global limiter of dry bean ( L.) production. In common bean and other legumes, resistance to FRR is related to both root development and root architecture, providing a breeding strategy for FRR resistance. Here, we describe the relationships between root traits and FRR disease symptoms. Using "shovelomics" techniques, a subset of recombinant inbred lines was phenotyped for root architecture traits and disease symptoms across three Michigan fields, including one field with artificially increased disease pressure. At the early growth stages, stem diameter, basal root number, and distribution of hypocotyl-borne adventitious roots were all significantly related to FRR disease scores. These results demonstrate that root architecture is a component of resistance to FRR in the field at early growth stages (first expanded trifoliate) complementing previous studies that evaluated root traits at later developmental stages (flowering, pod fill, etc.). Correlation matrices of root traits indicate that resistant and susceptible lines have statistically different root systems and show that basal root number is a key feature in resistant root systems while adventitious root distribution is an important feature in susceptible root systems. Based on the results of this study, selection for increased basal root number, increased adventitious root number, and even distribution of adventitious roots in early growth stages (first expanded trifoliate) would positively impact resistance to FRR.
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http://dx.doi.org/10.1094/PDIS-05-20-0956-REDOI Listing
December 2020

An automated microfluidic system for efficient capture of rare cells and rapid flow-free stimulation.

Lab Chip 2020 11;20(22):4246-4254

Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.

Cell fates are controlled by environmental stimuli that rapidly change the activity of intracellular signaling. Studying these processes requires rapid manipulations of micro-environmental conditions while continuously observing single cells over long periods of time. Current microfluidic devices are unable to simultaneously i) efficiently capture and concentrate rare cells, ii) conduct automated rapid media exchanges via diffusion without displacing non-adherent cells, and iii) allow sensitive high-throughput long-term time-lapse microscopy. Hematopoietic stem and progenitor cells pose a particular challenge for these types of experiments as they are impossible to obtain in very large numbers and are displaced by the fluid flow usually used to change culture media, thus preventing cell tracking. Here, we developed a programmable automated system composed of a novel microfluidic device for efficient capture of rare cells in independently addressable culture chambers, a custom incubation system, and user-friendly control software. The chip's culture chambers are optimized for efficient and sensitive fluorescence microscopy and their media can be individually and quickly changed by diffusion without non-adherent cell displacement. The chip allows efficient capture, stimulation, and sensitive high-frequency time-lapse observation of rare and sensitive murine and human primary hematopoietic stem cells. Our 3D-printed humidification and incubation system minimizes gas consumption, facilitates chip setup, and maintains stable humidity and gas composition during long-term cell culture. This approach now enables the required continuous long-term single-cell quantification of rare non-adherent cells with rapid environmental manipulations, e.g. of rapid signaling dynamics and the later stem cell fate choices they control.
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http://dx.doi.org/10.1039/d0lc00687dDOI Listing
November 2020

Production and Characterization of an Integrated Multi-Layer 3D Printed PLGA/GelMA Scaffold Aimed for Bile Duct Restoration and Detection.

Front Bioeng Biotechnol 2020 26;8:971. Epub 2020 Aug 26.

Department of Hepatobiliary Surgery, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, China.

We successfully fabricated artificial bile duct via 3D printing technique which was composed of poly (lactic-co-glycolic acid) (PLGA) and gelatin methacrylate (GelMA). The PLGA-inner layer provided sufficient strength to support the bile duct contraction, the GelMA-outer layer possessed good biocompatibility to provide a good living environment for the cells. Moreover, IKVAV laminin peptide (Ile-Lys-Val-Ala-Val) and ultrasmall superparamagnetic iron oxide (USPIO) were used to regulate scaffold cell adhesion and magnetic resonance imaging (MRI) detection, respectively. After BMSCs co-culture with IKVAV at a certain concentration, the survival rate and adhesion of BMSCs was increased obviously. Meanwhile, the fabricated scaffold exhibited the tensile modulus in the range of 17.19 - 29.05 MPa and the compressive modulus in the range of 0.042 - 0.066 MPa, which could meet the needs of human implantation. In an animal experiment pig bile duct regeneration, PLGA/GelMA/IKVAV/USPIO duct conduits could promote bile duct regeneration and enhance cytokeratin 19 (CK19) expression. In summary, the composite bile duct scaffold with excellent MRI imaging function and biocompatibility could be used to develop bioactive artificial bile ducts.
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http://dx.doi.org/10.3389/fbioe.2020.00971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479063PMC
August 2020

First successful treatment of a COVID-19 pregnant woman with severe ARDS by combining early mechanical ventilation and ECMO.

Heart Lung 2021 Jan - Feb;50(1):33-36. Epub 2020 Aug 21.

Department of Cardiovascular Center, Zhongshan People's Hospital, Zhongshan 528403, PR China. Electronic address:

The novel coronavirus (COVID-19) has become a global pandemic outbreak. Patients with COVID-19 are prone to progress to acute respiratory distress syndrome (ARDS), and even severe ARDS with ineffective mechanical ventilation, and an extremely high mortality. Extracorporeal membrane oxygenation (ECMO) provides effective respiratory support and saves time for the treatment of severe COVID-19. The present study reports that a 31-year-old pregnant female infected by COVID-19, who suffered from fever, dyspnea, and rapid ARDS. The patient's pulmonary function gradually recovered by combining early mechanical ventilation and ECMO, and finally, this patient was successfully weaned from ECMO and the ventilator. No fibrosis lesions were found in the chest CT, and the patient recovered very well after leaving from the hospital for one month.
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http://dx.doi.org/10.1016/j.hrtlng.2020.08.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441876PMC
December 2020

Sensitive biosensor for p53 DNA sequence based on the photothermal effect of gold nanoparticles and the signal amplification of locked nucleic acid functionalized DNA walkers using a thermometer as readout.

Talanta 2020 Dec 21;220:121398. Epub 2020 Jul 21.

MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. Electronic address:

A convenient photothermal biosensor was constructed for p53 DNA sequence detection based on the high discrimination capability of locked nucleic acid and high efficiency of signal amplification strategy of DNA walkers and difference photothermal effect between aggregated and dispersed gold nanoparticles (AuNPs). The presence of target activated the DNA walkers via the high affinity between target and complementary locked nucleic acid in the probe strand, resulting in the hybridization of the walker strand and substrate strand to form a specific enzyme recognition site. Under the cleavage of the endonuclease, single-stranded DNA (ssDNA) was released to the solution. Then the walker strand bound to a new substrate strand, and the next round of cleavage was triggered. The released ssDNA enhanced the stability of AuNPs against salt-induced aggregation. Given difference photothermal effects of the aggregated AuNPs and dispersed AuNPs under the near-infrared laser, the change of the temperature was detected by a common thermometer easily, which had a linear relationship with the target concentration in the range of 2.0-120.0 pM, the detection limit was 1.4 pM (S/N = 3). The proposed photothermal assay has been applied to detect p53 DNA sequence spiked complex samples with satisfying results.
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http://dx.doi.org/10.1016/j.talanta.2020.121398DOI Listing
December 2020

Evaluation of systemic lupus erythematosus disease activity using anti-α-enolase antibody and RDW.

Clin Exp Med 2021 Feb 28;21(1):73-78. Epub 2020 Aug 28.

Department of Laboratory Medicine, Zhongshan Hospital of Sun Yat-Sen University, 2 Sunwendong Road, Guangzhou, Guangdong Province, 528403, China.

The objective of the study was to investigate the value of anti-α-enolase antibody (Ab) combined with RDW in evaluating the activity of systemic lupus erythematosus (SLE). Levels of serum anti-α-enolase Ab and RDW were detected in 193 SLE patients and 98 healthy controls by ELISA and automatic blood cell counter (XN9000), respectively. Furthermore, the correlation between anti-α-enolase Ab and RDW in evaluating the activity of SLE was evaluated by correlation analysis. The level of anti-α-enolase Ab (9.16 ± 0.44 ng/mL in stable group and 10.26 ± 0.36 ng/mL in activity group) was significantly higher than that in the healthy control (7.05 ± 0.27 ng/mL). The level of RDW (12.92% ± 1.23% in stable group and 13.57% ± 2.12% in activity group) was significantly higher than that in the healthy control (12.46% ± 0.61%). The levels of anti-α-enolase Ab or RDW in SLE patients were positively correlated with SLEDAI-2 K score (r= 0.75, r = 0.73), respectively. Compared with the anti-α-enolase Ab (AUC: 78.0%) or RDW (AUC:80.0%) alone, anti-α-enolase Ab combined with RDW (AUC: 81.0%) had the best of the effectiveness of evaluating activity of SLE. These data suggested that combined anti-α-enolase Ab with RDW might be good biomarker to predict the activity of SLE in clinical.
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http://dx.doi.org/10.1007/s10238-020-00657-wDOI Listing
February 2021

Defect Engineering of Copper Paddlewheel-Based Metal-Organic Frameworks of Type NOTT-100: Implementing Truncated Linkers and Its Effect on Catalytic Properties.

ACS Appl Mater Interfaces 2020 Aug 11;12(34):37993-38002. Epub 2020 Aug 11.

Chair of Inorganic and Metal-Organic Chemistry, Department of Chemistry, Technical University of Munich, Lichtenbergtraße 4, Garching 85748, Germany.

A series of new defect-engineered metal-organic frameworks (DEMOFs) were synthesized by framework doping with truncated linkers employing the mixed-linker approach. Two tritopic defective (truncated) linkers, biphenyl-3,3',5-tricarboxylates (L) lacking a ligating group and 5-(5-carboxypyridin-3-yl)isophthalates (L) bearing a weaker interacting ligator site, were integrated into the framework of Cu(BPTC) (NOTT-100, BPTC = biphenyl-3,3',5,5'-tetracarboxylates). Incorporating L into the framework mainly generates missing metal node defects, thereby obtaining dangling COOH groups in the framework. However, introducing L forms more modified metal nodes featuring reduced and more accessible Cu sites. In comparison with the pristine NOTT-100, the defect-engineered NOTT-100 (DE-NOTT-100) samples show two unique features: (i) functional groups (the protonated carboxylate groups as the Brønsted acid sites or the pyridyl N atoms as the Lewis basic sites), which can act as second active sites, are incorporated into the MOF frameworks, and (ii) more modified paddlewheels, which provided extra coordinatively unsaturated sites, are generated. The cooperative functioning of the above characteristics enhances the catalytic performance of certain types of reactions. For a proof of concept, two exemplary reactions, namely, the cycloaddition of CO with propylene oxide to propylene carbonate and the cyclopropanation of styrene, were carried out to evaluate the catalytic activities of those DE-NOTT-100 materials depending on the defect structure.
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http://dx.doi.org/10.1021/acsami.0c07249DOI Listing
August 2020

CO adsorption on the calcite(10.4) surface: a combined experimental and theoretical study.

Phys Chem Chem Phys 2021 Apr 9;23(13):7696-7702. Epub 2020 Jul 9.

Karlsruher Institut für Technologie (KIT), Institut für Funktionelle Grenzflächen (IFG), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.

Detailed information on structural, chemical, and physical properties of natural cleaved (10.4) calcite surfaces was obtained by a combined atomic force microscopy (AFM) and infrared (IR) study using CO as a probe molecule under ultrahigh vacuum (UHV) conditions. The structural quality of the surfaces was determined using non-contact AFM (NC-AFM), which also allowed assigning the adsorption site of CO molecules. Vibrational frequencies of adsorbed CO species were determined by polarization-resolved infrared reflection absorption spectroscopy (IRRAS). At low exposures, adsorption of CO on the freshly cleaved (10.4) calcite surface at a temperature of 62 K led to the occurrence of a single C-O vibrational band located at 2175.8 cm, blue-shifted with respect to the gas phase value. For larger exposures, a slight, coverage-induced redshift was observed, leading to a frequency of 2173.4 cm for a full monolayer. The width of the vibrational bands is extremely small, providing strong evidence that the cleaved calcite surface is well-defined with only one CO adsorption site. A quantitative analysis of the IRRA spectra recorded at different surface temperatures revealed a CO binding energy of -0.31 eV. NC-AFM data acquired at 5 K for sub-monolayer CO coverage reveal single molecules imaged as depressions at the position of the protruding surface features, in agreement with the IRRAS results. Since there are no previous experimental data of this type, the interpretation of the results was aided by employing density functional theory calculations to determine adsorption geometries, binding energies, and vibrational frequencies of carbon monoxide on the (10.4) calcite surface. It was found that the preferred geometry of CO on this surface is adsorption on top of calcium in a slightly tilted orientation. With increased coverage, the binding energy shows a small decrease, revealing the presence of repulsive adsorbate-adsorbate interactions.
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http://dx.doi.org/10.1039/d0cp02698kDOI Listing
April 2021