Publications by authors named "Weihao Li"

104 Publications

The Synaptic Vesicle Protein 2A Interacts With Key Pathogenic Factors in Alzheimer's Disease: Implications for Treatment.

Front Cell Dev Biol 2021 1;9:609908. Epub 2021 Jul 1.

PET Center, Huashan Hospital, Fudan University, Shanghai, China.

Alzheimer's disease (AD), a serious neurodegenerative disease, is pathologically characterized by synaptic loss and dysfunction. Synaptic vesicle protein 2A (SV2A) is an indispensable vesicular protein specifically expressed in synapses and can be used as a biomarker for synaptic density. We found that the expression of SV2A was down-regulated in the hippocampus of AD patients, yet the relation of SV2A to other hallmarks of AD pathology such as amyloid precursor protein (APP), β-amyloid (Aβ), and Tau protein is not thoroughly clear. In addition, SV2A colocalized with APP and was down-regulated at Aβ deposition. Moreover, we found that SV2A deficiency leads to a simultaneous increase in Aβ and Tau hyperphosphorylation, while SV2A overexpression was associated with downregulation of β-site APP cleaving enzyme 1 and apolipoprotein E genes. In addition, evidence gained in the study points to the phosphatidylinositol 3-kinase signaling pathway as a possible mediator in SV2A regulation influencing the incidence and development of AD. With limited effective diagnostic methods for AD, a close interplay between SV2A and AD-related proteins demonstrated in our study may provide novel and innovative diagnostic and therapeutic opportunities.
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http://dx.doi.org/10.3389/fcell.2021.609908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282058PMC
July 2021

Ammonia exposure induces endoplasmic reticulum stress and apoptosis in Chinese striped-necked turtle (Mauremys sinensis).

Aquat Toxicol 2021 Jun 25;237:105903. Epub 2021 Jun 25.

Key Laboratory of Tropical Island Ecology, Ministry of Education, Hainan key Laboratory of Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou 571158, PR China. Electronic address:

Ammonia is a major pollutant in the water environment, which could cause severe harm to aquatic organisms. To explore the pathological and physiological effects of ammonia in Chinese striped-necked turtles (Mauremys sinensis), the individuals (body mass: 218.26 ± 12.65 g) were divided into two groups: control group and ammonia exposed group (6.25 mM total ammonia), then the expression levels of signaling factors involved in the endoplasmic reticulum stress and apoptotic pathways were determined. The results showed that ammonia exposure up-regulated the transcriptional and protein levels of endoplasmic reticulum stress marker gene Bip. Meanwhile, the relative mRNA levels of key genes (PERK, ATF6, eIF2α, ATF4, IRE1α and XBP1) involved in unfolded protein response up-regulated, and the phosphorylation levels of PERK, eIF2α and IRE1α increased correspondingly. In addition, the protein and transcriptional levels of CHOP and JNK related to apoptotic pathway induced by unfolded protein reaction increased under ammonia exposure. Moreover, Bcl-2 mRNA expression levels and protein levels decreased, whereas BAX and caspase-3 showed an opposite trend, and the cleaved protein of caspase-3 appeared when the turtles in the elevated ammonia. Furthermore, the apoptotic cells in liver increased after ammonia exposure. These results suggested ammonia exposure induced endoplasmic reticulum stress, then activated unfolded protein response, followed by apoptosis in M. sinensis. The results will contribute to a better understanding of the toxicity mechanism of ammonia to aquatic turtles.
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http://dx.doi.org/10.1016/j.aquatox.2021.105903DOI Listing
June 2021

All-optical polarization split of the signal and LO for a bi-directional self-homodyne coherent system.

Opt Lett 2021 Jun;46(12):2819-2822

In this Letter, we propose a cost-efficient bi-directional (BiDi) polarization-multiplexed self-homodyne coherent detection (SHCD) system, in which only one fiber link and one adaptive polarization controller (APC) are required. By employing the correlation of the state of polarization (SOP) between the upstream and downstream light, one APC is capable of stabilizing SOPs of the counterpropagating waves at the same time. The signal and local oscillator (LO) can be optically split by a polarization beam splitter (PBS), relaxing pressure of the digital signal processing (DSP) and simplifying the coherent receiver. The impact induced by polarization cross talk and delay mismatch is collectively investigated by theoretical analysis and simulation. Finally, the proposed scheme is experimentally verified through 120 Gbit/s 16-quadrature amplitude modulation (16-QAM) transmission, achieving a satisfying laser-linewidth tolerance of 10 MHz and a polarization rotation tolerance of up to 45 rad/s.
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http://dx.doi.org/10.1364/OL.427789DOI Listing
June 2021

Method, Design, and Evaluation of an Exoskeleton for Lifting a Load In Situ.

Appl Bionics Biomech 2021 25;2021:5513013. Epub 2021 May 25.

School of Mechanical and Materials Engineering, North China University of Technology, Beijing 100144, China.

Due to the unclear application scenarios and force analysis of exoskeletons, there exists a research gap in exoskeleton design. This paper presents a design method and realization of an exoskeleton for a specific scenario of lifting a load in situ. Firstly, the lifting motion process and its data were collected based on a 3-D motion capture system and dynamometer treadmill system. Then, the variations of the torque and motion of each joint were obtained from the data analysis, based on which an active assistance mode for upper limbs and a passive assistance mode for lower limbs were demonstrated. In this design, the hydraulic cylinder for shoulder assistance, the motor for elbow assistance, and the spring for lower limb assistance were calculated and selected according to the motion and torque of each joint. Finally, subjective and objective methods were used to evaluate the exoskeleton based on the results of five test participants, and the median oxygen consumption of the whole test by lifting a load ten times with the assistance was found to be reduced by 9.45% as compared with that in the absence of the exoskeleton.
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http://dx.doi.org/10.1155/2021/5513013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169279PMC
May 2021

The diagnostic value of a non-contrast computed tomography scan-based radiomics model for acute aortic dissection.

Medicine (Baltimore) 2021 Jun;100(22):e26212

Department of Radiology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine.

Abstract: To investigate the diagnostic value of a computed tomography (CT) scan-based radiomics model for acute aortic dissection.For the dissection group, we retrospectively selected 50 patients clinically diagnosed with acute aortic dissection between October 2018 and November 2019, for whom non-contrast CT and CT angiography images were available. Fifty individuals with available non-contrast CT and CT angiography images for other causes were selected for inclusion in the non-dissection group. Based on the aortic dissection locations on the CT angiography images, we marked the corresponding regions-of-interest on the non-contrast CT images of both groups. We collected 1203 characteristic parameters from these regions by extracting radiomics features. Subsequently, we used a random number table to include 70 individuals in the training group and 30 in the validation group. Finally, we used the Lasso regression for dimension reduction and predictive model construction. The diagnostic performance of the model was evaluated by a receiver operating characteristic (ROC) curve.Fourteen characteristic parameters with non-zero coefficients were selected after dimension reduction. The accuracy, sensitivity, specificity, and area under the ROC curve of the prediction model for the training group were 94.3% (66/70), 91.2% (31/34), 97.2% (35/36), and 0.988 (95% confidence interval [CI]: 0.970-0.998), respectively. The respective values for the validation group were 90.0% (27/30), 94.1% (16/17), 84.6% (11/13), and 0.952 (95% CI: 0.883-0.986).Our non-contrast CT scan-based radiomics model accurately facilitated acute aortic dissection diagnosis.
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http://dx.doi.org/10.1097/MD.0000000000026212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183783PMC
June 2021

Flat hardness distribution in AA6061 joints by linear friction welding.

Sci Rep 2021 Jun 3;11(1):11756. Epub 2021 Jun 3.

Joining and Welding Research Institute, Osaka University, Osaka, Ibaraki, 567-0047, Japan.

It is known that one of the main concerns associated with the conventional welding of precipitation-strengthened Al alloys is the formation of softening regions, resulting in the deterioration of mechanical properties. In this study, we show that linear friction welding (LFW) can completely suppress softening regions in precipitation-strengthened AA6061-T6 alloy by introducing a large shear strain and by controlling the interfacial temperature. We found that the LFW process resulted in an extremely low interfacial temperature; it decreased as the applied pressure increased from 50 to 240 MPa. This approach can essentially suppress both softening and hardening regions, leading to uniform hardness distribution in Al joints. The high-pressure LFW process demonstrated here can thus provide an innovated guidance to obtain high-performance Al alloy joints and be extended to other precipitation-strengthened Al alloys, which undergo high-temperature softening.
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http://dx.doi.org/10.1038/s41598-021-91249-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175386PMC
June 2021

Stroke outcome assessment: Optimizing cutoff scores for the Longshi Scale, modified Rankin Scale and Barthel Index.

PLoS One 2021 13;16(5):e0251103. Epub 2021 May 13.

The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, Guangdong, China.

The Longshi Scale, a visual-based scale, is reliable and valid in activity assessment, but lacks cutoff definition corresponding to classical scales such as the modified Rankin Scale and Barthel Index. Therefore, this study aimed to investigate the relationships of the Longshi Scale with the modified Rankin Scale and Barthel Index and optimize cutoff scores of these scales in stroke outcomes assessment. This is a cross-sectional study. Stroke patients were measured concurrently by the Longshi scale, modified Rankin Scale and Barthel Index. Kruskal-Wallis test and Spearman correlation analysis were used to analyze the differences and associations among the three scales. The receiver operating characteristic curve was performed to determine the optimal cutoff scores. A total of 5475 stroke patients (67.3% ischemic) were included in this study. There are close relationships of the Longshi Scale with adjusted modified Rankin Scale and Barthel Index (r = -0.861, 0.922; p<0.001, <0.001; respectively). The activity levels assessed by adjusted modified Rankin Scale and Barthel Index among different Longshi scale grades were significantly different (χ2:4217.27, 4676.55; p<0.001, <0.001; respectively). The optimal cutoff scores were adjusted modified Rankin Scale 4, 3, 3, 3, 2 for Longshi scale grade 2 to 6 (sensitivity%: 96.12, 70.24, 89.10, 96.80, 86.23, specificity%: 72.72, 98.29, 93.81, 79.82, 92.89, respectively), and Barthel Index 15, 45, 60, 75, 80 for Longshi scale grade 2 to 6 (sensitivity%: 92.54, 89.28, 91.32, 90.30, 95.65, specificity%: 95.48, 89.51, 94.02, 90.41, 90.62, respectively). In conclusion, the classification of Longshi Scale is consistent with those of modified Rankin Scale and Barthel Index. We recommend the Longshi Scale as an effective supplement for modified Rankin Scale and Barthel Index in assessing the outcome in acute stroke patients.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251103PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118543PMC
May 2021

Handgrip Strength-Related Factors Affecting Health Outcomes in Young Adults: Association with Cardiorespiratory Fitness.

Biomed Res Int 2021 21;2021:6645252. Epub 2021 Apr 21.

The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China.

Objectives: Handgrip strength (HS) is a risk factor of all-cause mortality and cardiovascular diseases. However, the influencing factors and mechanisms contributing to this correlation remain unclear. Therefore, we aimed to explore factors related to HS and investigated the mechanism underlying its risk predictive value.

Methods: This was a prospective, cross-sectional study. One hundred forty-five participants were recruited from December 2019 to November 2020. HS was measured using a hydraulic hand dynamometer and adjusted for body mass index (HS) and body surface area (HS). Body composition was assessed via bioimpedance spectroscopy. Physical fitness was measured using a cardiopulmonary exercise test system. Univariate, multiple linear regression analyses and receiver operator characteristic curve (ROC) were conducted to evaluate the associations between various participant characteristics and HS.

Results: The average participant age was 21.68 ± 2.61 years (42.8% were male). We found positive correlations between HS/HS and VO, VE, Load, and MET in both sexes ( < 0.05). Lean-tissue, protein, total water, and inorganic salt percentages were positively correlated, and fat percentage was negatively correlated with HS in men and with HS and HS in women ( < 0.05). Multiple regression revealed that VO was independently associated with HS in both sexes ( = 0.215, 0.173; 95%confidence interval [CI] = 0.032 - 0.398, 0.026-0.321; = 0.022, 0.022, respectively) and independently associated with HS in women ( = 0.016, 95%CI = 0.004 - 0.029, = 0.011). ROC analysis showed that HS and HS can moderately identify normal VO in men (area under curve [AUC] = 0.754, 0.769; = 0.002, 0.001, respectively) and marginally identify normal VO in women (AUC = 0.643, 0.635; = 0.029, 0.042, respectively).

Conclusions: BMI- and BSA-adjusted HS could serve as indicators of physical health, and HS may moderately reflect cardiorespiratory fitness levels in healthy young adults, particularly in males. Clinical trials registry site and number: China Clinical Trial Center (ChiCTR1900028228).
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http://dx.doi.org/10.1155/2021/6645252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084643PMC
May 2021

Mid-term outcomes of endovascular treatment and risk factors for recurrence in patients with Trans-Atlantic-Inter-Society II C/D femoropopliteal lesions.

Quant Imaging Med Surg 2021 May;11(5):2028-2039

Department of Interventional Radiology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: The purpose of this study was to determine the efficacy of interventional endovascular treatment (EVT) and the risk factors for postoperative restenosis in patients with Trans-Atlantic Inter-Society Consensus (TASC) II C/D femoropopliteal lesions.

Methods: A total of 55 patients who received EVT for TASC II C/D femoropopliteal lesions (64 affected limbs) from October 2014 to September 2017 were examined. The mean lesion length was 19.6±5.3 (range, 15.5-26.4 cm). The femoropopliteal lesions were classified as TASC II C and TASC II D in 39 and 16 cases, respectively. The ankle-brachial index (ABI), primary patency rate, secondary patency rate, and limb salvage rate were monitored in follow-up evaluations for up to 24 months. A Cox regression model was used to evaluate the correlation between each of these factors and the restenosis rate after EVT.

Results: Patent TASC II C/D femoropopliteal lesions were present in 59 of the 64 limbs. The mean ABI values for the dorsal pedal artery and posterior tibial artery increased 1 month after treatment from a baseline level of 0.35±0.12 to 0.89±0.10 and from 0.43±0.15 to 0.90±0.13, respectively (P<0.01). The mean follow-up time was 19.3 (range, 6-24) months. The cumulative primary patency rates at 1, 3, 6, 12, 18, and 24 months were 98.3%, 91.5%, 84.3%, 61.1%, 53.1%, and 31.1%, respectively. The secondary patency rates at 12 and 24 months were 70.4% and 60.0%, respectively. Factors with a high hazard ratio included male sex, TASC II D, smoking, and diabetes mellitus (DM).

Conclusions: EVT had a safe and satisfactory mid-term therapeutic effect on TASC II C/D femoropopliteal lesions. Male sex, TASC II D (compared to TASC II C), smoking, and DM were risk factors for restenosis. EVT has a secondary patency rate comparable to that of open surgery and can be considered a first-line treatment for TASC II C/D femoropopliteal lesions.
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http://dx.doi.org/10.21037/qims-20-221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047376PMC
May 2021

Intermittent hypoxia-induced downregulation of microRNA-320b promotes lung cancer tumorigenesis by increasing CDT1 via USP37.

Mol Ther Nucleic Acids 2021 Jun 1;24:528-541. Epub 2021 Jan 1.

Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. China.

Obstructive sleep apnea-hypopnea (OSAH) is correlated with an increased incidence of lung cancer. In our study, we explored the functional roles of microRNAs (miRNAs) in lung cancer patients that were complicated with OSAH involving the deubiquitination enzyme. The miR-320b expression pattern in lung cancer tissues and cells was determined. The interactions between ubiquitin-specific peptidase 37 (USP37) and miR-320b were evaluated by a dual-luciferase reporter gene assay, whereas USP37 and Cdc10-dependent transcript 1 (CDT1) was assessed by co-immunoprecipitation and immunofluorescence. After the induction of intermittent hypoxia (IH), a gain-of function approach was performed to investigate roles of miR-320b, USP37, and CDT1 in lung cancer cell proliferation and invasion. In addition, nude mouse xenograft models were used to study their effects on tumor growth . miR-320b was poorly expressed in lung cancer patients with OSAH. IH treatment downregulated the expression of miR-320b but promoted the proliferation and invasion capabilities of lung cancer cells, both of which were suppressed by the overexpression of miR-320b through decreasing USP37. USP37 interacted with and deubiquitinated CDT1 to protect it from proteasomal degradation. Our study uncovered that IH-induced downregulation of miR-320b promoted the tumorigenesis of lung cancer by the USP37-mediated deubiquitination of CDT1.
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http://dx.doi.org/10.1016/j.omtn.2020.12.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056179PMC
June 2021

Prognostic value of fast semi-automated left atrial long-axis strain analysis in hypertrophic cardiomyopathy.

J Cardiovasc Magn Reson 2021 03 25;23(1):36. Epub 2021 Mar 25.

Cardiology Division, Department of Medicine West China Hospital, Sichuan University, Guoxue Xiang No. 37, Chengdu, 610041, Sichuan, China.

Background: The prognostic value of left atrial (LA) size and function in hypertrophic cardiomyopathy (HCM) is well recognized, but LA function is difficult to routinely analyze. Fast LA long-axis strain (LA-LAS) analysis is a novel technique to assess LA function on cine cardiovascular magnetic resonance (CMR). We aimed to assess the association between fast LA-LAS and adverse clinical outcomes in patients with HCM.

Methods: 359 HCM patients and 100 healthy controls underwent routine CMR imaging. Fast LA-LAS was analyzed by automatically tracking the length between the midpoint of posterior LA wall and the left atrioventricular junction based on standard 2- and 4-chamber balanced steady-state free precession cine-CMR. Three strain parameters including reservoir strain (εs), conduit strain (εe), and active strain (εa) were assessed. The endpoint was set as composite adverse events including cardiovascular death, resuscitated cardiac arrest, sudden cardiac death aborted by appropriate implantable cardioverter-defibrillator discharge, and hospital admission related to heart failure.

Results: During an average follow-up of 40.9 months, 59 patients (19.7%) reached endpoints. LA strains were correlated with LA diameter, LA volume index (LAVI) and LA empty fraction (LAEF) (all p < 0.05). In the stepwise multivariate Cox regression analysis, εs and εe (hazard ratio, 0.94 and 0.89; p = 0.019 and 0.006, respectively) emerged as independent predictors of the composite adverse events. Fast LA εs and LA εe are stronger prognostic factors than LA size, LAVI and the presence of left ventricular late gadolinium enhancement.

Conclusions: Fast LA reservoir and conduit strains are independently associated with adverse outcomes in HCM.
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http://dx.doi.org/10.1186/s12968-021-00735-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992961PMC
March 2021

The anatomic distribution and pulmonary embolism complications of hospital-acquired lower extremity deep venous thrombosis.

J Vasc Surg Venous Lymphat Disord 2021 Mar 19. Epub 2021 Mar 19.

Department of Vascular Surgery, Peking University People's Hospital, Peking University, Beijing, China. Electronic address:

Objective: The anatomic distribution of lower extremity deep venous thrombosis (LEDVT) plays an important role in its prevention and treatment. This study aimed to evaluate the anatomic distribution of hospital-acquired LEDVT (HA-LEDVT) and its probable role in the occurrence of pulmonary embolism (PE).

Methods: We retrospectively analyzed the demographic data, ultrasound results, and PE-related findings of inpatients with HA-LEDVT in 28 clinical departments at Peking University People's Hospital between January 1, 2007, and December 31, 2018.

Results: This study included 1431 HA-LEDVT events: 35.8%, 31%, and 33.3% were left, right, and bilateral LEDVT. Isolated distal, proximal, and blended DVT were detected in 83.4%, 7.3%, and 9.3% of the patients, respectively. The distribution of HA-LEDVT in the left and right lower extremities were not significantly different except in patients aged ≥40 years (left: 2.07 vs right: 1.88 per 1000 extremities, P = .04). For anatomic types of HA-LEDVT, isolated distal HA-LEDVT was 5.02 times more prevalent than proximal HA-LEDVT (1.24 vs 0.26 per 1000 extremities, P < .01). The involvement rates of specific deep veins by HA-LEDVT were highest in the muscular calf vein (87.5%) followed by the popliteal vein (10.1%), superficial femoral vein (9.3%), and common femoral vein (9.2%). HA-LEDVT involving multiple vein segments simultaneously occurred in 338 extremities. HA-LEDVT involving the muscular calf vein and at least one of three connected axial veins of the muscular calf vein occurred most frequently. Eighty-eight patients with HA-LEDVT (6.15%) had PE. The frequency of PE among patients with proximal and distal DVT (7.89% vs 6.23% P = .275) was not significantly different. The incidence of PE was highest in patients with bilateral proximal DVT (15.4%) and lowest in patients with a single right distal DVT (4.5%). PE occurred in 6% of muscular calf vein HA-LEDVT. In isolated muscular calf vein DVT cases, PE were more likely to occur in cases with a >6.05-mm-diameter thrombus than in those with a <6.05-mm-diameter thrombus (10.3% vs 4.2%, P < .0001).

Conclusions: HA-LEDVT is characterized by a significantly high percentage of DVT in the muscular calf vein. Muscular calf vein thrombosis may be the primary origin of lower extremity deep vein thrombosis. The diameter of the thrombus in the muscular calf vein may be associated with the occurrence of PE. More prospective studies are needed to more fully determine the natural history of HA-LEDVT and develop prevention and treatment guidelines for HA-LEDVT.
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http://dx.doi.org/10.1016/j.jvsv.2021.03.004DOI Listing
March 2021

The effect of Chinese herbal medicine on digestive system and liver functions should not be neglected in COVID-19: An updated systematic review and meta-analysis.

IUBMB Life 2021 05 2;73(5):739-760. Epub 2021 Apr 2.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Gastrointestinal symptoms and liver injury are common in patients with coronavirus disease 2019 (COVID-19). However, profiles of different pharmaceutical interventions used are relatively underexplored. Chinese herbal medicine (CHM) has been increasingly used for patients with COVID-19, but the efficacy of CHM used in COVID-19 on gastrointestinal symptoms and liver functions has not been well studied with definitive results based on the updated studies. The present study aimed at testing the efficacy of CHM on digestive symptoms and liver function (primary outcomes), the aggravation of COVID-19, and the time to viral assay conversion (secondary outcomes), among patients with COVID-19, compared with standard pharmacotherapy. The literature search was undertaken in 11 electronic databases from December 1, 2019 up to November 8, 2020. Appraisal of the evidence was conducted with Cochrane risk of bias tool or Newcastle Ottawa Scale. A random-effects model or subgroup analysis was conducted when significant heterogeneity was identified in the meta-analysis. The certainty of the evidence was assessed with the grading of recommendations assessment, development, and evaluation approach. Forty-eight included trials involving 4,704 participants were included. Meta-analyses favored CHM plus standard pharmacotherapy for COVID-19 on reducing the aggravation of COVID-19 and the time to viral assay conversion compared with standard pharmacotherapy. However, the present CHM as a complementary therapy for treating COVID-19 may not be beneficial for improving most gastrointestinal symptoms and liver function based on the current evidence. More well-conducted trials are warranted to confirm the potential efficacy of CHM furtherly.
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http://dx.doi.org/10.1002/iub.2467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250823PMC
May 2021

Prognostic value of estimated glomerular filtration rate and presence of proteinuria in type B aortic intramural hematoma.

J Thorac Dis 2021 Feb;13(2):946-954

Department of Vascular Surgery, Peking University People's Hospital, Beijing, China.

Background: Although aggressive medical treatment is recommended in patients with type B aortic intramural hematoma (IMH), a variety of aortic events can occur during the later period. For early identification of these patients, the present study was aimed at evaluating the prognostic validity of estimated glomerular filtration rate (eGFR) and the presence of proteinuria in type B aortic IMH.

Methods: Data of 61 patients with type B IMH in Peking University People's Hospital from January 2008 to December 2018 were retrospectively collected. The serum creatinine level and urine protein levels were measured at admission. And eGFR were calculated by the CKD-EPI equation. Adverse aortic-related events were defined as a composite of satisfaction of criteria for surgical conversion (with or without actual surgical intervention) and death from aortic rupture.

Results: Initial eGFR was significantly different between patients with adverse aortic-related events and those without (P=0.003). On multivariate analysis, eGFR <90 mL/min/1.73 m (OR, 8.726; 95% CI: 1.711-46.144; P=0.009) and ULP (OR, 17.516; 95% CI: 3.322-92.258; P=0.001) were independent predictors of adverse aorta-related events. Furthermore, eGFR <90 mL/min/1.73 m and proteinuria (+) (OR, 8.344; P=0.030) had significantly greater rates of aortic-related events. In addition, eGFR <90 mL/min/1.73 m and proteinuria (+) had incremental prognostic value (C-statistic, 0.860, P=0.039) compared with ulcer-like projection (C-statistic, 0.815) alone.

Conclusions: Initial eGFR and presence of proteinuria were able to provide incremental prognostic information in addition to ulcer-like projection in patients with type B aortic IMH.
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http://dx.doi.org/10.21037/jtd-20-2543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947484PMC
February 2021

Acoustic vibration technology: Toward a promising fruit quality detection method.

Compr Rev Food Sci Food Saf 2021 Mar 15;20(2):1655-1680. Epub 2021 Feb 15.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

Understanding fruit quality is an effective way to reduce fruit loss and waste by providing proper strategies for fruit processing and management. Various noninvasive technologies have been developed for assessing fruit quality. Among them, acoustic vibration technology has received considerable attention from academics. As the fruit ripens, the physical and biochemical diversities of fruit cell lead to changes in the vibration characteristics of the fruit, which verified the feasibility of assessing fruit firmness by acoustic vibration technology from the microscopic view. Besides, the acoustic and vibration theories also provided the theoretical basis for this technology. The measurement system was mainly consisted of excitation devices, detection sensors, and signal processing modules. By using different excitation methods and devices, the fruit could demonstrate a free or forced vibration. The response signals of fruit were influenced by detection methods and sensors. To meet the requirement of high-throughput detection, noncontact excitation devices and detection sensors were more suitable for on-line applications. In addition, the relative locations of excitation and detection points and the posture style of fruit also had an impact on the measurement results, which should be determined before the test. Moreover, the proper data analysis method was equally important to extract potential parameters to improve the performance of prediction models. This paper provided a comprehensive overview concerning the principle, system composition, data analysis, and on-line applications of acoustic vibration technology, and discussed the perspectives of future trends to promote the intensive study and further extension of this technology.
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http://dx.doi.org/10.1111/1541-4337.12722DOI Listing
March 2021

Phenotyping of myocardial involvement by cardiac magnetic resonance in idiopathic inflammatory myopathies.

Eur Radiol 2021 Jul 6;31(7):5077-5086. Epub 2021 Jan 6.

Department of Medicine, Cardiovascular Division, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Chengdu, 610041, Sichuan, China.

Objectives: Cardiac dysfunction is commonly noted in patients with idiopathic inflammatory myopathies (IIMs). This study aimed to investigate the characteristics of cardiac dysfunction using cardiac magnetic resonance (CMR) in polymyositis (PM), dermatomyositis (DM) and necrotising myositis (NM).

Methods: Fifty-one patients with IIMs and 20 matched healthy controls (HCs) were assessed using CMR examination. The clinical data, cardiac serum markers and autoimmune antibodies were determined for all patients. Cardiac involvement was identified by myocardial native T1, extracellular volume (ECV), late gadolinium enhancement (LGE) and left ventricular ejection fraction (LVEF).

Results: Different subtypes of IIMs showed different patterns of LGE and varying degrees of myocardial damage. The PM subgroup showed higher native T1 (p = 0.010) and ECV (p = 0.000) than the HCs. The prevalence of LGE was comparable between the PM and DM subgroups (40.0% vs. 31.6%, p = 0.741); however, it was higher in the PM subgroup than in the NM subgroup (40% vs. 0.0%, p = 0.014). Patients with positive LGE in the PM subgroup showed a higher proportion of positive LGE (p = 0.018) and lower LVEF (p = 0.024) than those with positive LGE in the DM subgroup. In multivariate analysis, the presence of LGE could be predicted by increased NT-proBNP (p = 0.036, OR = 1.001) and anti-MDA-5 antibody positivity (p = 0.011, OR = 12.4). The risk factors associated with native T1 were NT-proBNP (p = 0.016, β = 0.353) and body mass index (BMI) (p = 0.024, β = - 0.331).

Conclusions: Distinct cardiac involvements in different subtypes of IIMs were identified using CMR. Elevated NT-proBNP and a low BMI were the risk factors associated with LGE and elevated native T1.

Key Points: • The characteristics of cardiac involvement in different subtypes of IIMs could be identified with cardiac magnetic resonance. • The NT-proBNP levels could reflect focal and diffuse myocardial damage in patients with IIMs.
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http://dx.doi.org/10.1007/s00330-020-07448-7DOI Listing
July 2021

A stacking-based model for predicting 30-day all-cause hospital readmissions of patients with acute myocardial infarction.

BMC Med Inform Decis Mak 2020 12 14;20(1):335. Epub 2020 Dec 14.

Cardiology Division, West China Hospital, Sichuan University, No.17 People's South Road,Chengdu, 610041, Chengdu, Sichuan, PR China.

Background: Acute myocardial infarction (AMI) is a serious cardiovascular disease, followed by a high readmission rate within 30-days of discharge. Accurate prediction of AMI readmission is a crucial way to identify the high-risk group and optimize the distribution of medical resources.

Methods: In this study, we propose a stacking-based model to predict the risk of 30-day unplanned all-cause hospital readmissions for AMI patients based on clinical data. Firstly, we conducted an under-sampling method of neighborhood cleaning rule (NCR) to alleviate the class imbalance and then utilized a feature selection method of SelectFromModel (SFM) to select effective features. Secondly, we adopted a self-adaptive approach to select base classifiers from eight candidate models according to their performances in datasets. Finally, we constructed a three-layer stacking model in which layer 1 and layer 2 were base-layer and level 3 was meta-layer. The predictions of the base-layer were used to train the meta-layer in order to make the final forecast.

Results: The results show that the proposed model exhibits the highest AUC (0.720), which is higher than that of decision tree (0.681), support vector machine (0.707), random forest (0.701), extra trees (0.709), adaBoost (0.702), bootstrap aggregating (0.704), gradient boosting decision tree (0.710) and extreme gradient enhancement (0.713).

Conclusion: It is evident that our model could effectively predict the risk of 30-day all cause hospital readmissions for AMI patients and provide decision support for the administration.
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http://dx.doi.org/10.1186/s12911-020-01358-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734833PMC
December 2020

Oridonin triggers G2/M cell cycle arrest, cellular apoptosis and autophagy in human gastric cancer cells.

J BUON 2020 Sep-Oct;25(5):2308-2314

Department of Gastrointestinal Surgery, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Xianning 437100, China.

Purpose: Gastric carcinoma is the fourth principal cause of cancer-related deaths throughout the globe. There are inadequate clinical therapies for gastric cancer due to lack of operational drugs and ambiguity in molecular mechanisms. As such there is a persistent requirement for novel and effective anticancer drugs for gastric cancer. The main purpose of the current study was to investigate the antitumor effects of a plant diterpenoid, namely Oridonin, against SGC-7901 human gastric cancer cells along with examining its effects on cellular apoptosis, cell autophagy and cell cycle phase distribution.

Methods: WST-1 cell proliferation assay was used to evaluate cell viability of SGC-7901 human gastric cancer cells. Apoptosis was evaluated by using DAPI and comet assays using fluorescence microscopy. Autophagy was evaluated by transmission electron microscopy (TEM) and western blot method. Effects on cell cycle phase distribution were studied by flow cytometry.

Results: Oridonin molecule led to considerable and dose-dependent antiproliferative effects on SGC-7901 human gastric cancer cells exerting only mild cytotoxic effects in normal cells thus exhibiting selective toxicity. The number of gastric cancer cell colonies decreased significantly as oridonin dose increased. DAPI and comet assays revealed that oridonin induced powerful apoptotic effects in these cells, triggering significant DNA damage and both these effects exhibited dose-dependence. TEM indicated that oridonin induced autophagy in SGC-7901 cells by creating autophagosomes and autophagic vacuoles. Oridonin also targeted G2/M phase cell cycle in these gastric cancer cells along with targeting some key cell cycle related proteins including cyclin-B1, cyclin D1 and cyclin E.

Conclusion: In conclusion, the results show that oridonin showed strong anticancer effects in SGC-7901 human gastric cancer cells by triggering apoptosis and autophagy, and targeting cell cycle at G2/M phase.
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December 2020

Myocardial Tissue Reverse Remodeling After Guideline-Directed Medical Therapy in Idiopathic Dilated Cardiomyopathy.

Circ Heart Fail 2021 01 13;14(1):e007944. Epub 2020 Nov 13.

Department of Cardiology (Y.X., W.L., Y. Liang, X.J., J.W., Y. Li, S.T., J.G., X.G., Q.Z., Y.C.), West China Hospital, Sichuan University, Chengdu, China.

Background: The prognosis of patients with idiopathic dilated cardiomyopathy (DCM) has improved remarkably in recent decades with guideline-directed medical therapy. Left ventricular (LV) reverse remodeling (LVRR) is one of the major therapeutic goals. Whether myocardial fibrosis or inflammation would reverse associated with LVRR remains unknown.

Methods: A total of 157 prospectively enrolled patients with DCM underwent baseline and follow-up cardiovascular magnetic resonance examinations with a median interval of 13.7 months (interquartile range, 12.2-18.5 months). LVRR was defined as an absolute increase in LV ejection fraction of >10% to the final value of ≥35% and a relative decrease in LV end-diastolic volume of >10%. Statistical analyses were performed using paired test and student test, logistic regression analysis, and linear regression analysis.

Results: Forty-eight (31%) patients reached LVRR. At baseline, younger age, worse New York Heart Association class, new-onset heart failure, lower LV ejection fraction, absence of late gadolinium enhancement, lower myocardial T2, and extracellular volume were significant predictors of LVRR. During the follow-up, patients with and without LVRR both showed a significant decrease of myocardial native T1 (LVRR: [baseline] 1303.0±43.6 ms; [follow-up] 1244.7±51.8 ms; without LVRR: [baseline] 1308.5±80.5 ms; [follow-up] 1287.6±74.9 ms, both <0.001), matrix and cellular volumes while no significant difference was observed in T2 or extracellular volume values after treatment.

Conclusions: In patients with idiopathic DCM, the absence of late gadolinium enhancement, lower T2, and extracellular volume values at baseline are significant predictors of LVRR. The myocardial T1, matrix, and cell volume decrease significantly in patients with LVRR after guideline-directed medical therapy. Registration: URL: https://www.clinicaltrials.gov; Unique identifier: ChiCTR1800017058.
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http://dx.doi.org/10.1161/CIRCHEARTFAILURE.120.007944DOI Listing
January 2021

Upregulated ox40l Can Be Inhibited by miR-146a-5p in Condylar Chondrocytes Induced by IL-1β and TNF-α: A Possible Regulatory Mechanism in Osteoarthritis.

Int Arch Allergy Immunol 2021 4;182(5):408-416. Epub 2020 Nov 4.

Department of Dental Research, The Affiliated Stomatology Hospital of Kunming Medical University, Kunming, China,

Introduction: Osteoarthritis (OA) is a common musculoskeletal disease characterized by pain, stiffness, limited activity, occasional effusion, and local inflammation. MiR-146 is one of the noncoding RNA closely related to OA, but the role of miR-146 in OA remains controversial. The tumour necrosis factor receptor OX40 is activated by its cognate ligand OX40L (TNFSF4) and functions as a T-cell costimulatory molecule. The T-cell functions, including cytokine production, expansion, and survival, are enhanced by the OX40 costimulatory signals.

Methods: We established an inflammatory model of condylar chondrocytes induced by IL-1β and TNF-α and detected the expression of miRNA by miRNA sequencing. Then, cell transfection was used to study the role of miR146a-5p in OA. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and database analysis were used to screen out potential target genes of miR-146a-5p. A dual luciferase activity assay tested whether ox40l is the target gene of miR-146a-5p.

Results: MiR-146a-5p and OX40L was upregulated after induced by IL-1β and TNF-α, miR-146a-5p reduced the production of inflammatory factors but had no effect on chondrophenotypic factors, and ox40l was targeted by miR-146a-5p.

Conclusion: OX40L and miR-146a-5p of condylar chondrocytes in the inflammatory environment (induced by IL-1β and TNF-α) were significantly increased, miR-146a-5p is a protective factor in the inflammatory response, which can reduce the production of inflammatory factors, and miR-146a-5p may regulate T-cell-mediated immunity through targeting of ox40l in OA.
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http://dx.doi.org/10.1159/000512291DOI Listing
November 2020

Feasibility Study of a Modified XELOX Adjuvant Chemotherapy for High-Recurrence Risk Patients With Operated Stage III Colon Cancer.

Front Pharmacol 2020 18;11:583091. Epub 2020 Sep 18.

Department of Colorectal Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background: Our previous study reported the favorable efficacy and good tolerance associated with a modified XELOX adjuvant chemotherapy with eight cycles of capecitabine and six cycles of oxaliplatin for operated stage III colon cancer. The current study aimed to confirm the feasibility of modified XELOX chemotherapy for treating specific high-risk (T4, N2, or both) stage III colon cancer.

Methods: We selected 142 consecutive patients with high-risk stage III colon cancer who received colon tumor resection followed by modified XELOX or standard full-cycle XELOX chemotherapy from November 2007 to June 2016 at Sun Yat-sen University Cancer Center. Disease-free survival (DFS), overall survival (OS), and adverse events of patients treated with the two chemotherapy regimens were compared.

Results: Seventy-four (52.1%) patients received standard XELOX chemotherapy, and 68 (47.8%) received modified XELOX chemotherapy. Neurotoxicity was the most common adverse event in 99 (69.7%) patients. Grade 2-3 neurotoxicity, grade 2-4 thrombocytopenia and grade 3-4 leucopenia were the major severe adverse events related to the decision to treat patients with modified XELOX chemotherapy. After a median follow-up of 69 months, the modified XELOX group presented a comparable 5-year DFS rate (79.0 vs. 80.3%, P = 0.891) and 5-year OS rate (93.8 vs. 87.8%, P = 0.446) as those in the standard XELOX group. Univariate survival analysis indicated that poor tumor differentiation (HR: 2.381, 95% CI: 1.141-4.968, P = 0.021) was the only significant risk factor for DFS, but no significant prognostic factor was identified for OS.

Conclusions: The modified XELOX adjuvant chemotherapy presented a comparable oncologic efficacy as standard XELOX chemotherapy for high-risk stage III colon cancer. The modified XELOX adjuvant chemotherapy could be an alternative treatment for patients suffering severe adverse events, especially severe neurotoxicity.
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http://dx.doi.org/10.3389/fphar.2020.583091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7543913PMC
September 2020

Tubal ectopic pregnancy occurrence is associated with high expressions of prokineticin receptors and aberrant secretion of inflammatory cytokines.

Am J Transl Res 2020 15;12(9):5741-5751. Epub 2020 Sep 15.

The Reproductive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University Shenzhen 510000, Guangdong Province, China.

Objective: Tubal ectopic pregnancy (TEP) remains the most common cause of maternal morbidity and mortality in the early months of pregnancy. The aim of this study is to perform the correlation between PROKRs and pro-inflammatory genes and explore the role of novel genes in pathogenesis of TEP.

Methods: Here, quantitative real time PCR and immunohistochemistry were used to assess the expression of the novel genes in 120 TEP patients and 30 age-matched non-TEP patients. The correlation between PROKRs and pro-inflammatory genes were analyzed by Pearson correlation coefficient. Univariate and multivariate Cox regression analyses were used to assess the risk prediction rate of novel genes. Receiver operating characteristic was used to assess the performance of our model.

Results: PROKRs (PROKR1 and PROKR2) and pro-inflammatory genes (TNF-α, IL-6, and IL-8) expression levels significantly enhanced in TEP patients, and significantly positive correlation with pro-inflammatory genes for PROKRs. A multivariate Cox regression analysis demonstrated that 2 genes (PROKR2 and IL8) had significant diagnostic value, which were associated with the occurrence and development of TEP.

Conclusion: Our data further denote that dysregulation of PROKR2 and IL-8 were risk factor and played an important role in the pathogenesis of TEP.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540125PMC
September 2020

Modulation of the intestinal barrier adaptive functions in red-eared slider (Trachemys scripta elegans) invading brackish waters.

Sci Total Environ 2021 Jan 19;751:141744. Epub 2020 Aug 19.

Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China. Electronic address:

Globally, the increase in sea levels is leading to salinization of freshwater, which might influence the freshwater organisms such as red-eared slider, Trachemys scripta elegans. The turtle can invade brackish water environments, in which it must deal with elevated salinity in the gastrointestinal tract that could impact the intestinal function. The intestinal barrier provides a front-line of organismal defense against the chemical and biological environmental insults. In this study, the adaptive functions of the intestinal barrier including intestinal histomorphology, genes involved in intestinal barrier functions, and the intestinal micro-ecosystem were analyzed in the turtles exposed to freshwater (S0), 5‰ salinity (S5) and 15‰ salinity (S15) water for 30 days. The results showed that the intestine of T. s. elegans maintained normal histomorphological structure in the S5 group, whereas the villus height, crypt depth and the number of goblet cells in the S15 group were lower than that in the S5 and S0 groups. In addition, the relative expression levels of epithelial tight junction-related genes and intestinal immune-related genes in the gut were significantly upregulated in the S15 group, compared to the freshwater group. Mucin-2 gene expression was downregulated, but mucin-1 transcript levels were upregulated in salinity-treated groups. Furthermore, the abundances of phylum Proteobacteria, and genera Morganella and Aeromonas in the intestine were particularly enhanced in the S15 group than the S0 and S5 groups. Taken together, these results indicate that the intestinal barrier plays a protective role in T. s. elegans adaptation to brackish water environments. Our results provide a perspective on the evolution of salinity tolerance and help to evaluate the potential danger of the turtle to other species, and understand the challenges that other species must meet with rising sea levels.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141744DOI Listing
January 2021

Propofol disrupts cell carcinogenesis and aerobic glycolysis by regulating circTADA2A/miR-455-3p/FOXM1 axis in lung cancer.

Cell Cycle 2020 10 28;19(19):2538-2552. Epub 2020 Aug 28.

Department of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan, China.

The involvement of propofol and circular RNAs (circRNAs) in lung cancer progression has been identified. However, the relationship between propofol and circRNAs as well as the underlying molecular mechanisms on lung cancer development remain unclear. Cell viability, migration and invasion were measured by cell counting kit-8 assay, 5-bromo-2-deoxyuridine (BrdU) and transwell assay. Glycolytic metabolism was calculated by measuring the glucose consumption, lactate production and extracellular acidification. Western blot was used to detect the protein of glucose transporter 1 (GLUT1), glycolysis enzymes, and forkhead box M1 (FOXM1). The expression of circRNA transcriptional adaptor 2A (circTADA2A), microRNA (miR)-455-3p and FOXM1 mRNA was detected by quantitative real-time polymerase chain reaction. The interaction between miR-455-3p and circTADA2A or FOXM1 was analyzed using the dual-luciferase reporter assay. Murine xenograft model was established to perform i experiments. We found propofol treatment alleviated lung cancer cell proliferation, migration, invasion and aerobic glycolysis as well as inhibited tumor growth . Propofol decreased the level of circTADA2A and exerted anti-tumor effects by regulating circTADA2A. MiR-455-3p directly interacted with circTADA2A and FOXM1 in lung cancer cells, and circTADA2A could regulate FOXM1 expression by binding to miR-455-3p. Subsequently, rescue assay showed that propofol inhibited cell proliferation, migration, invasion and aerobic glycolysis by regulating circTADA2A/miR-455-3p/FOXM1 axis in lung cancer. Collectively, propofol suppressed cell carcinogenesis and aerobic glycolysis by regulating circTADA2A/miR-455-3p/FOXM1 axis in lung cancer, providing an effective clinical implication for propofol to prevent the development of lung cancer.
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http://dx.doi.org/10.1080/15384101.2020.1810393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553507PMC
October 2020

Dcf1 deletion presents alterations in gut microbiota of mice similar to Parkinson's disease.

Biochem Biophys Res Commun 2020 09 3;529(4):1137-1144. Epub 2020 Aug 3.

Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, Shanghai, 200444, China. Electronic address:

The gut-brain communication is increasingly being recognized as a profound effector on Parkinson's disease (PD). Gut microbiota changes have become the focus of attention. However, the mechanism leading to changes in the gut microbiota is not clear. In the present study, we found that knockout of Dcf1 (Dcf1) caused changes in the gut microbiota in mice. Results indicated that the increased Proteobacteria (phylum-level) and decreased Prevotellaceae (family-level) in the microbiota composition of Dcf1 (KO) mice, which is consistent with the situation of PD patients. On species-level, Prevotellaceae_UCG-001 and Helicobacter_ganmani were significantly different between KO and WT mice, suggesting glycolipid metabolism disorders and inflammatory lesions in KO mice. In the behavior of Y-maze and Open field test, KO mice showed typical PD symptoms such as memory deficits, slowness of movement and anxiety. Further Nissl staining of brain tissue sections confirmed that the deletion of Dcf1 caused damage to amygdala neurons. These results provide a new mechanism for understanding gut microbiota changes, and provide a new basis for PD treatment from a new perspective of Gut-brain axis.
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http://dx.doi.org/10.1016/j.bbrc.2020.06.150DOI Listing
September 2020

Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression.

J Exp Clin Cancer Res 2020 Jul 22;39(1):140. Epub 2020 Jul 22.

Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan Province, People's Republic of China.

Background: Exosomal microRNAs (miRNAs or miRs) from bone marrow-derived mesenchymal stem cells (UCMSCs) have emerged as promising therapeutic strategies for cancer treatment. The current study aimed to elucidate the underlying mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs)-derived exosomal miR-375 in esophageal squamous cell carcinoma (ESCC).

Methods: After determining the expression of miR-375 and its putative target enabled homolog (ENAH) in ESCC tissues and cells, we tested effects of their altered expression on ESCC proliferation, invasion, migration, and tumorsphere formation was subsequently measured. Transfected hUCMSCs-derived exosomes (hUCMSCs-exo) were isolated and co-cultured with ESCC cells to measure the effects of miR-375 delivered by hUCMSCs-exo on ESCC development. Finally, we investigated the effect of miR-375 on tumor growth in vivo.

Results: The expression of miR-375 was reduced, while the expression of ENAH was elevated in ESCC. ENAH was identified as a target gene of miR-375. Elevated miR-375 or depleted ENAH expression inhibited ESCC cell proliferation, invasion, migration, tumorsphere formation, and promoted apoptosis. Moreover, miR-375 delivered by hUCMSCs-exo could suppress ESCC cell proliferation, invasion, migration, tumorsphere formation, but promoted apoptosis in vitro, as well as inhibiting tumor growth in vivo.

Conclusions: Taken together, hUCMSCs-exo can deliver miR-375 to suppress ENAH expression and subsequently inhibit the initiation and progression of ESCC.
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http://dx.doi.org/10.1186/s13046-020-01631-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374920PMC
July 2020

Prognostic Value of Right Ventricular Dysfunction in Patients With AL Amyloidosis: Comparison of Different Techniques by Cardiac Magnetic Resonance.

J Magn Reson Imaging 2020 11 20;52(5):1441-1448. Epub 2020 Jul 20.

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.

Background: Right ventricular (RV) dysfunction is common in patients with amyloid light-chain (AL) amyloidosis. While cardiac MRI is the reference standard tool for RV assessment, there are a number of measures of RV function that can be evaluated and it is yet unknown which of these results in the highest prognostic performance in AL amyloidosis.

Purpose: To examine the prognostic value of various measures of RV function in a bid to find which best predicts outcome in AL amyloidosis.

Study Type: Single-center, prospective.

Subjects: In all, 129 patients (mean age, 58 ± 11 years; 61.2% men) with biopsy-proven AL amyloidosis.

Field Strength/sequence: 3.0T / balanced steady-state free-precession cine.

Assessment: RV ejection fraction (EF), RV fractional area change (FAC), RV long axis strain (LAS), RV free wall longitudinal strain (FWS), RV global longitudinal strain (GLS), and tricuspid annular plane systolic excursion (TAPSE).

Statistical Tests: Mann-Whitney U-tests, Student's t-tests, receiver-operating characteristic curves, Kaplan-Meier curves, Cox proportional hazards regression models, and C-statistics.

Results: During the median follow-up period of 38.0 months (interquartile range, 18.5-58.0 months), all-cause mortality occurred in 95 patients (73.6%). The RVEF, RVGLS, TAPSE, RVFAC, and RVFWS were significant predictors of outcome in univariate Cox regression (all P < 0.001). After adjusting for New York Heart Association (NYHA) class, Mayo staging 2004, left ventricular (LV) EF, and LV mass index, RVFWS (HR [hazard ratio] =1.074; 95% CI [confidence interval]: 1.041-1.108; P < 0.001) was an independent predictor of all-cause mortality and had a higher C-statistic (0.753) compared to the model including RVEF (C-statistic = 0.724, P = 0.034), the model including RVFAC (C-statistic = 0.723, P = 0.033), and the model including RVGLS (C-statistic =0.733, P = 0.011).

Data Conclusion: RV dysfunction appears to be an independent determinant of outcome in patients with AL amyloidosis. RVFWS is a better predictor of all-cause mortality than RVEF, RVFAC, or RVGLS.

Evidence Level: 2 TECHNICAL EFFICACY STAGE: 5.
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http://dx.doi.org/10.1002/jmri.27200DOI Listing
November 2020

Prognostic value of left ventricular remodelling index in idiopathic dilated cardiomyopathy.

Eur Heart J Cardiovasc Imaging 2020 Jul 13. Epub 2020 Jul 13.

Department of Cardiology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Chengdu, Sichuan 610041, China.

Aims: To evaluate the prognostic value of left ventricular (LV) remodelling index (RI) in idiopathic dilated cardiomyopathy (DCM) patients.

Methods And Results: We prospectively enrolled 412 idiopathic DCM patients and 130 age- and sex-matched healthy volunteers who underwent cardiovascular magnetic resonance imaging between September 2013 and March 2018. RI was defined as the cubic root of the LV end-diastolic volume divided by the mean LV wall thickness on basal short-axis slice. The primary endpoint included all-cause mortality and heart transplantation. The secondary endpoint included the primary endpoint and heart failure (HF) readmission. During the median follow-up of 28.1 months (interquartile range: 19.3-43.0 months), 62 (15.0%) and 143 (34.7%) patients reached the primary and secondary endpoints, respectively. Stepwise multivariate Cox regression showed that RI [hazard ratio (HR) 1.20, 95% confidence interval (CI) 1.11-1.30, P < 0.001], late gadolinium enhancement (LGE) presence and log (N-terminal pro-B-type natriuretic peptide) were independent predictors of the primary endpoint, while RI (HR 1.15, 95% CI 1.08-1.23, P < 0.001) and extracellular volume were independent predictors of the secondary endpoint. The addition of RI to LV ejection fraction (EF) and LGE presence showed significantly improved global χ2 for predicting primary and secondary endpoints (both P < 0.001). Furthermore, RI derived from echocardiography also showed independent prognostic value for primary and secondary endpoints with clinical risk factors.

Conclusions: RI is an independent predictor of all-cause mortality, heart transplantation, and HF readmission in DCM patients and provides incremental prognostic value to LVEF and LGE presence.
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http://dx.doi.org/10.1093/ehjci/jeaa144DOI Listing
July 2020

Dcf1 alleviates C99-mediated deficits in drosophila by reducing the cleavage of C99.

Biochem Biophys Res Commun 2020 09 12;530(2):410-417. Epub 2020 Jun 12.

Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, Shanghai, 200444, China. Electronic address:

Alzheimer's disease (AD) is a progressive neurodegenerative disorder. The generation of amyloid-β from the amyloid precursor protein (APP) C-terminal fragment (C99) by γ-secretase cleavage is one of the main pathological mechanisms of AD. Dendritic cell factor 1 (Dcf1) is a membrane protein that was previously found to play a role in the development of AD. Bioinformatic analysis of AD patients indicated that Dcf1 may affect γ-secretase. In this study, we confirmed that Dcf1 attenuates the cleavage of C99 in vivo and in vitro. By using C99 transgenic AD drosophila, we found that Dcf1 reduces the cleavage of C99 by γ-secretase using Dcf1 overexpression. The climbing ability and lifespan of C99 drosophila were significantly increased, while learning and memory were also enhanced with Dcf1 expression. Increased levels of C99 protein in Dcf1-AD drosophila reveals inhibition of C99 cleavage by Dcf1 in vivo. Dcf1 inhibition of γ-secretase was further confirmed in vitro. These results provide a potential therapeutic target for the treatment of AD and also propose a new mechanism for understanding the occurrence of AD.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.063DOI Listing
September 2020

Rapid detection of antibiotic resistance genes in lactic acid bacteria using PMMA-based microreactor arrays.

Appl Microbiol Biotechnol 2020 Jul 2;104(14):6375-6383. Epub 2020 Jun 2.

Handan Municipal Centre for Disease Control and Prevention, Handan, 056000, Hebei Province, China.

The emergence of lactic acid bacteria (LABs) resistant to existing antimicrobial drugs is a growing health crisis. To decrease the overuse of antibiotics, molecular diagnostic systems that can rapidly determine the presence of antibiotic resistance (AR) genes in LABs from yogurt samples are needed. This paper describes a fully integrated, miniaturized plastic chip and closed-tube detection chemistry that performs multiplex nucleic acid amplification. High-throughput identification of AR genes was achieved through this approach, and six AR genes were analyzed simultaneously in < 2 h. This time-to-result included the time required for the extraction of DNA. The detection limit of the chip was 10 CFU mL, which was consistent with that of tube LAMP. We detected and identified multiple DNAs, including streptomycin, tetracycline, and vancomycin resistance-associated genes, with complete concordance to the Kirby-Bauer disk diffusion method.Key Points• A miniaturized chip was presented, and multiplex nucleic acid amplification was performed.• The device can be integrated with LAMP for rapid detection of antibiotic resistance genes.• The approach had a high throughput of AR gene analysis in lactic acid bacteria.
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http://dx.doi.org/10.1007/s00253-020-10699-5DOI Listing
July 2020
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