Publications by authors named "Weiguo Wang"

186 Publications

Antibacterial Activity of Aureonuclemycin Produced by Strain SPRI-371.

Molecules 2022 Aug 8;27(15). Epub 2022 Aug 8.

Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.

Actinomycetes play a vital role as one of the most important natural resources for both pharmaceutical and agricultural applications. The actinomycete strain SPRI-371, isolated from soil collected in Jiangsu province, China, was classified as based on its morphological, physiological, biochemical and molecular biological characteristics. Its bacterial activity metabolites were identified as aureonuclemycin (ANM), belonging to adenosine derivatives with the molecular formula CHNO for ANM A and CHNO for ANM B. Simultaneously, the industrial fermentation process of a mutated strain SPRI-371 was optimized in a 20 m fermentation tank, featuring a rotation speed of 170 rpm, a pressure of 0.05 MPa, an inoculum age of 36-40 h and a dissolved oxygen level maintained at 1-30% within 40-80 h and at >60% in the later period, resulting in an ANM yield of >3700 mg/L. In the industrial separation of fermentation broth, the sulfuric acid solution was selected to adjust pH and 4# resin was used for adsorption. Then, it was resolved with 20% ethanol solution and concentrated in a vacuum (60-65 °C), with excellent results. Antibacterial experiments showed that ANM was less active or inactive against pv. , subsp. and pv. and most bacteria, yeast and fungi in vitro. However, in vivo experiments showed that ANM exhibited extremely significant protective and therapeutic activity against diseases caused by pv. and pv. in rice and in oranges and lemons. In field trials, ANM A 150 gai/ha + ANM B 75 gai/ha exhibited excellent therapeutic activity against rice bacterial leaf blight, citrus canker and rice bacterial leaf streak. Furthermore, as the dosage and production cost of ANM are lower than those of commercial drugs, it has good application prospects.
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http://dx.doi.org/10.3390/molecules27155041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370760PMC
August 2022

The Value of Combined Detection of Megakaryocyte and Platelet Parameters for the Diagnosis of Primary Immune Thrombocytopenia.

Clin Appl Thromb Hemost 2022 Jan-Dec;28:10760296221106779

Department of Laboratory Medicine, the Second People's Hospital of Lianyungang City, Jiangsu Province, China.

To investigate the application value of bone marrow megakaryocyte count, the proportion of megakaryocytes at each stage, and the platelet parameter in the clinical diagnosis of primary immune thrombocytopenia (ITP). The megakaryocyte and platelet parameter level in 62 ITP and 40 control group patients were compared and analyzed. Linear correlation analysis, Pearson correlation analysis, and ROC curves were performed for the correlation between megakaryocytes and platelet parameters. Compared to the control group, the total number of megakaryocytes, the promegakaryocytes the granular megakaryocytes (GMeg), and naked megakaryocytes (NMeg), MPV, and P-LCR% in the ITP group increased. All differences were statistically significant (<0.05). While the proportion of platelet-producing megakaryocytes (PMeg), PLT, and PCT decreased in the ITP group. These differences were statistically significant ( 0.05). PLT was strongly positively correlated with PCT (r = 0.921, p<0.01). PCT was weakly positively with MPV (r = 0.309, p<0.05). MPV was positively correlated with P-LCR (r = 0.856, p<0.01). PDW was weakly positively correlated with P-LCR (r = 0.296, p<0.05) and Meg (r = 0.301, p<0.05), and negatively correlated with PMeg (r = -0.336, p<0.05). ROC curve analysis showed that PLT, PCT MPV and P-LCR% gave a high sensitivity(100.0%,81.0%,74.6%,90.5%,respectively.) and specificity (100.0%, 92.5%, 80.0%, 77.5%, respectively.) in diagnosis of ITP. The combined analysis of bone marrow megakaryocyte count, the proportion of megakaryocyte classification at each stage, and platelet parameters have an important reference value for auxiliary diagnosis of ITP.
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http://dx.doi.org/10.1177/10760296221106779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358600PMC
August 2022

Sensor Assessment of Gap Balance in Mobile-Bearing Unicompartmental Knee Arthroplasty.

J Vis Exp 2022 07 12(185). Epub 2022 Jul 12.

Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences; Department of Orthopaedic Surgery, China-Japan Friendship Hospital; Department of Orthopaedic Surgery, Peking University China-Japan Friendship School of Clinical Medicine;

The most important procedure of mobile-bearing unicompartmental knee arthroplasty (UKA) is to balance the knee flexion and extension gap. Conventionally, the balance was determined by the subjective assessment of plugging out the feeling gauge. Since it mainly depended on the surgeons' experience, the accuracy was always in doubt. In the past 10 years, pressure sensors have been introduced to guide the gap balance in total knee arthroplasty (TKA). However, the sensor technique was introduced to UKA very recently. Herein is our sensor assessment of the gap balance in 20 cases UKA by one experienced surgeon. The sensor was a custom-designed force sensor matrix according to the shape of the tibial trial of mobile-bearing UKA. The postoperative clinical outcomes and radiographic results were recorded for future comparison. We aim to use this method to assess more than 200 cases of UKA by various surgeons to ultimately standardize the gap-balance result.
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http://dx.doi.org/10.3791/63711DOI Listing
July 2022

Unicompartmental Knee Arthroplasty for Knee Osteoarthritis With the Pellegrini-Stieda Lesion: A Case Report.

Front Surg 2022 6;9:922896. Epub 2022 Jul 6.

Department of Orthopaedic Surgery, China-Japan Friendship Hospital, Beijing, China.

Pellegrini-Stieda lesion is described as ossification on the origin of medial collateral ligament. We present a case of end-stage knee osteoarthritis with the Pellegrini-Stieda lesion treated by unicompartmental knee arthroplasty (UKA). During the postoperative follow-up, an interesting change occurred such that the ossification lesion disappeared gradually and did not relapse. It is supposed that the disappearance was caused by UKA changing the abnormal biomechanics of the varus osteoarthritic knee.
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http://dx.doi.org/10.3389/fsurg.2022.922896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301303PMC
July 2022

PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3.

Am J Transl Res 2022 15;14(6):4295-4309. Epub 2022 Jun 15.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University Nanjing 210046, China.

Embryonic stem cell (ESC) research is critical to the scientific community, as their application in regenerative medicine can be widely beneficial. ESCs eventually withdraw from their self-renewal program and subsequently differentiate into specific cell lineages; however, the mechanisms regulating these processes remain unclear. PKC inhibition using 3-[1-[3-(dimethylamino) propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione (PKCi) is responsible for the derivation and maintenance of human, rat, and mouse ESCs, but the mechanism by which PKCi maintains stem cell self-renewal is poorly understood. By studying the PKCi stem cell (PKCi-mESC) transcriptome and epigenetic modification, we found the transcriptome of PKCi-mESC differed from 2i stem cells (2i-mESC), with 2010 up-regulated genes and 1784 down-regulated genes. Among them, genes related to core transcription factors, naïve-specific markers, and pluripotency are differentially expressed between the two stem cell lines. We analyzed epigenetic modification of PKCi-mESC and found the distribution of H3K27me3 signal was significantly reduced at transcription start sites (TSSs) throughout the genome and at differentially expressed genes (DEGs). Likewise, the H3K9me3 signal at TSSs throughout the genome was significantly reduced in PKCi-mESC, but the distribution on DEGs is reversed. and knockdown by RNA interference (RNAi) significantly altered the expression of genes related to self-renewal in PKCi-mESC. In conclusion, we revealed PKCi-mESC and 2i-mESC differentially express numerous genes, including stem cell-related genes. Furthermore, PKCi-mESC regulated gene expression through H3K27me3 and H3K9me3 modification, which maintained stem cell self-renewal capacity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274548PMC
June 2022

Extracellular Vesicles from BMSCs Prevent Glucocorticoid-Induced BMECs Injury by Regulating Autophagy via the PI3K/Akt/mTOR Pathway.

Cells 2022 07 3;11(13). Epub 2022 Jul 3.

Department of Orthopaedic Surgery, Center for Osteonecrosis and Joint Preserving & Reconstruction, China-Japan Friendship Hospital, Beijing 100029, China.

Osteonecrosis of the femoral head (ONFH) is a common clinical disease with a high disability rate. Injury of bone microvascular endothelial cells (BMECs) caused by glucocorticoid administration is one of the important causes of ONFH, and there is currently a lack of effective clinical treatments. Extracellular vesicles derived from bone stem cells (BMSC-EVs) can prevent ONFH by promoting angiogenesis and can inhibit cell apoptosis by regulating autophagy via the PI3K/Akt/mTOR signaling pathway. The present study aimed to investigate the effect of extracellular vesicles derived from bone marrow stem cells (BMSC) on a glucocorticoid-induced injury of BMECs and possible mechanisms. We found that BMSC-EVs attenuated glucocorticoid-induced viability, angiogenesis capacity injury, and the apoptosis of BMECs. BMSC-EVs increased the LC3 level, but decreased p62 (an autophagy protein receptor) expression, suggesting that BMSC-Exos activated autophagy in glucocorticoid-treated BMECs. The protective effects of BMSC-EVs on the glucocorticoid-induced injury of BMECs was mimicked by a known stimulator of autophagy (rapamycin) and could be enhanced by co-treatment with an autophagy inhibitor (LY294002). BMSC-EVs also suppressed the PI3K/Akt/mTOR signaling pathway, which regulates cell autophagy, in glucocorticoid-treated BMECs. In conclusion, the results indicate that BMSC-EVs prevent the glucocorticoid-induced injury of BMECs by regulating autophagy via the PI3K/Akt/mTOR pathway.
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http://dx.doi.org/10.3390/cells11132104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265732PMC
July 2022

A radiographic model predicting the status of the anterior cruciate ligament in varus knee with osteoarthritis.

BMC Musculoskelet Disord 2022 Jun 22;23(1):603. Epub 2022 Jun 22.

Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Purpose: The study aims to investigate the accuracy of different radiographic signs for predicting functional deficiency of anterior cruciate ligament (ACL) and test whether the prediction model constructed by integrating multiple radiographic signs can improve the predictive ability.

Methods: A total number of 122 patients from January 1, 2018, to September 1, 2021, were enrolled in this study. Among them, 96 patients were classified as the ACL-functional (ACLF) group, while 26 patients as the ACL-deficient (ACLD) group after the assessment of magnetic resonance imaging (MRI) and the Lachman's test. Radiographic measurements, including the maximum wear point of the proximal tibia% (MWPPT%), tibial spine sign (TSS), coronal tibiofemoral subluxation (CTFS), hip-knee-ankle angle (HKA), mechanical proximal tibial angle (mPTA), mechanical lateral distal femoral angle (mLDFA) and posterior tibial slope (PTS) were measured using X-rays and compared between ACLF and ACLD group using univariate analysis. Significant variables (p < 0.05) in univariate analysis were further analyzed using multiple logistic regression analysis and a logistic regression model was also constructed by multivariable regression with generalized estimating models. Receiver-operating-characteristic (ROC) curve and area under the curve (AUC) were used to determine the cut-off value and the diagnostic accuracy of radiographic measurements and the logistic regression model.

Results: MWPPT% (odds ratio (OR) = 1.383, 95% confidence interval (CI) = 1.193-1.603, p < 0.001), HKA (OR = 1.326, 95%CI = 1.051-1.673, p = 0.017) and PTS (OR = 1.981, 95%CI = 1.207-3.253, p = 0.007) were shown as predictive indicators of ACLD, while age, sex, side, TSS, CTFS, mPTA and mLDFA were not. A predictive model (risk score = -27.147 + [0.342*MWPPT%] + [0.282*HKA] + [0.684*PTS]) of ACLD using the three significant imaging indicators was constructed through multiple logistic regression analysis. The cut-off values of MWPPT%, HKA, PTS and the predictive model were 52.4% (sensitivity:92.3%; specificity:83.3%), 8.5° (sensitivity: 61.5%; specificity: 77.1%), 9.6° (sensitivity: 69.2%; specificity: 78.2%) and 0.1 (sensitivity: 96.2%; specificity: 79.2%) with the AUC (95%CI) values of 0.906 (0.829-0.983), 0.703 (0.574-0.832), 0.740 (0.621-0.860) and 0.949 (0.912-0.986) in the ROC curve.

Conclusion: MWPPT% (> 52.4%), PTS (> 9.6°), and HKA (> 8.5°) were found to be predictive factors for ACLD, and MWPPT% had the highest sensitivity of the three factors. Therefore, MWPPT% can be used as a screening tool, while the model can be used as a diagnostic tool.
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http://dx.doi.org/10.1186/s12891-022-05568-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215084PMC
June 2022

RNA adenosine modifications related to prognosis and immune infiltration in osteosarcoma.

J Transl Med 2022 05 14;20(1):228. Epub 2022 May 14.

Department of Obstetrics and Gynecology, The Third Xiangya Hospital of Central South University, 138, Tongzipo Road, Changsha, 410013, China.

Background: RNA adenosine modifications, which are primarily mediated by "writer" enzymes (RMWs), play a key role in epigenetic regulation in various biological processes, including tumorigenesis. However, the expression and prognostic role of these genes in osteosarcoma (OS) remain unclear.

Methods: Univariate and multivariate Cox analyses were used to construct the RMW signature for OS using Target datasets. RMW expression in OS tissue was detected by qPCR analysis. Xcell and GSVA were used to determine the relationship between RMWs and immune infiltration. The DGIdb and CMap databases were used for drug prediction. In vivo and in vitro experiments showed that strophanthidin elicited antitumor activity against OS.

Results: A 3-RMW (CSTF2, ADAR and WTAP) prognostic signature in OS was constructed using the Target dataset and verified using GEO datasets and 63 independent OS tissues via qPCR analysis. High-risk OS patients had poor overall survival, and the prognostic signature was an independent prognostic factor for OS. Functional studies showed that tumour-, metabolism-, cell cycle- and immune-related pathways were related to high risk. Next, we found that RMW-derived high-risk patients exhibited increased infiltration of M2 macrophages and cDCs. Furthermore, we predicted the potential drugs for OS using the DGIdb and CMap databases. In vivo and in vitro experiments showed that strophanthidin elicited antitumor activity against OS by repressing cell growth and inducing cell cycle arrest at the G1 phase.

Conclusion: The 3-RWM-based prognostic signature established in this study is a novel gene signature associated with immune infiltration, and strophanthidin was identified as a candidate therapy for OS by repressing OS cell growth and the cell cycle.
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http://dx.doi.org/10.1186/s12967-022-03415-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107650PMC
May 2022

Augmented Reality Navigation-Guided Core Decompression for Osteonecrosis of Femoral Head.

J Vis Exp 2022 04 12(182). Epub 2022 Apr 12.

Department of Orthopedic Surgery, Beijing Key Lab of Immunes-Mediated Inflammatory Disease, China-Japan Friendship Hospital;

Osteonecrosis of the femoral head (ONFH) is a common joint disease in young and middle-aged patients, which seriously burdens their lives and work. For early-stage ONFH, core decompression surgery is a classical and effective hip preservation therapy. In traditional procedures of core decompression with Kirschner wire, there are still many problems such as X-ray exposure, repeated puncture verification, and damage to normal bone tissue. The blindness of the puncture process and the inability to provide real-time visualization are crucial reasons for these problems. To optimize this procedure, our team developed an intraoperative navigation system on the basis of augmented reality (AR) technology. This surgical system can intuitively display the anatomy of the surgical areas and render preoperative images and virtual needles to intraoperative video in real-time. With the guide of the navigation system, surgeons can accurately insert Kirschner wires into the targeted lesion area and minimize the collateral damage. We conducted 10 cases of core decompression surgery with this system. The efficiency of positioning and fluoroscopy is greatly improved compared to the traditional procedures, and the accuracy of puncture is also guaranteed.
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http://dx.doi.org/10.3791/63806DOI Listing
April 2022

Sensitive detection of glyoxal by cluster-mediated CHBr chemical ionization time-of-flight mass spectrometry.

Anal Chim Acta 2022 May 17;1206:339612. Epub 2022 Feb 17.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, China; Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China. Electronic address:

Direct and rapid analysis of glyoxal by soft ionization mass spectrometry remains a great challenge due to its low ionization efficiency in existing soft ionization techniques, such as proton transfer reaction (PTR) and photoionization (PI). In this work, we developed a new VUV lamp-based cluster-mediated CHBr chemical ionization (CMCI) source for time-of-flight mass spectrometry (TOFMS), which was accomplished by employing photoionization-generated CHBr as the reactant ion and co-sampling of glyoxal with high-concentration ethanol (EtOH). The signal intensity of glyoxal could be enhanced by more than 2 orders of magnitude by generating protonated cluster ion [Glx·EtOH·H]. Density function theory (DFT) calculations was performed to obtain the most stable structure of neutral glyoxal-ethanol cluster and confirm that the ionization energy (IE) of glyoxal-ethanol cluster was significantly lower than that of glyoxal and CHBr molecules, which makes it possible for effective ionization of glyoxal. The ionization efficiency of glyoxal could be dramatically enhanced via ion-molecule reaction between CHBr and glyoxal-ethanol cluster, as larger ionization cross section of glyoxal-ethanol cluster than glyoxal molecule might be achieved. The cluster-mediated signal enhanced effect was also verified by using other alcohols, such as methanol and isopropanol. Consequently, the limit of quantitation (LOQ, S/N = 10) down to 0.17 ppbv for gas-phase glyoxal was achieved. The analytical capacity of this system was demonstrated by trace analysis of glyoxal in food contact papers, exhibiting new insights and wide potentials of chemical ionization and photoionization mass spectrometry for VOCs measurement with higher sensitivity and wider detectable sample range.
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http://dx.doi.org/10.1016/j.aca.2022.339612DOI Listing
May 2022

Network Pharmacological Study on Mechanism of the Therapeutic Effect of Modified Duhuo Jisheng Decoction in Osteoporosis.

Front Endocrinol (Lausanne) 2022 31;13:860649. Epub 2022 Mar 31.

First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.

Background: Modified Duhuo Jisheng Decoction (MDHJSD) is a traditional Chinese medicine prescription for the treatment of osteoporosis (OP), but its mechanism of action has not yet been clarified. This study aims to explore the mechanism of MDHJSD in OP through a combination of network pharmacology analysis and experimental verification.

Methods: The active ingredients and corresponding targets of MDHJSD were acquired from the Traditional Chinese Medicine System Pharmacology (TCMSP) database. OP-related targets were acquired from databases, including Genecards, OMIM, Drugbank, CTD, and PGKB. The key compounds, core targets, major biological processes, and signaling pathways of MDHJSD that improve OP were identified by constructing and analysing the relevant networks. The binding affinities between key compounds and core targets were verified using AutoDock Vina software. A rat model of ovariectomized OP was used for the experimental verification.

Results: A total of 100 chemical constituents, 277 targets, and 4734 OP-related targets of MDHJSD were obtained. Subsequently, five core components and eight core targets were identified in the analysis. Pathway enrichment analysis revealed that overlapping targets were significantly enriched in the tumour necrosis factor-alpha (TNF-α) signaling pathway, an inflammation signaling pathway, which contained six of the eight core targets, including TNF-α, interleukin 6 (IL-6), transcription factor AP-1, mitogen-activated protein kinase 3, RAC-alpha serine/threonine-protein kinase, and caspase-3 (CASP3). Molecular docking analysis revealed close binding of the six core targets of the TNF signaling pathway to the core components. The results of experimental study show that MDHJSD can protect bone loss, inhibit the inflammatory response, and downregulate the expression levels of TNF-α, IL-6, and CASP3 in ovariectomized rats.

Conclusion: The mechanism of MDHJSD in the treatment of OP may be related to the regulation of the inflammatory response in the bone tissue.
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http://dx.doi.org/10.3389/fendo.2022.860649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008312PMC
April 2022

Alterations of the Subchondral Bone in Osteoarthritis: Complying with Wolff's law.

Curr Rheumatol Rev 2022 Apr 1. Epub 2022 Apr 1.

Department of Orthopaedic Surgery, China-Japan Friendship Hospital, No. 2 Yinghua East Road, Chaoyang District, 100029, Beijing, P.R.China.

Osteoarthritis (OA) is a whole joint disease that is significantly related to abnormal mechanical loads. Subchondral bone alterations, during the evolution course of OA, are considered as a reflection of adaptation of the bone tissue to mechanical loads. However, some of these alterations are taken as detriment and paradoxical. What are these structure, composition, and mechanical property alterations or mechanical functions for are not quite clear. In this review, we evaluate the possibility that these alterations are used for maintaining joint function. With taking excessive load as a risk factor and under conditions of articular cartilage gradually loss its thickness and its function of evenly distributing load on subchondral bone plate, and applying poroelasticity. Moreover, Boussinesq's pressure bulb theory and bone optimal design principles are utilized for bone mechanics. We found that each subchondral bone alteration has its unique mechanical function in resisting loads and maintaining joint function, and these alterations comply with both bone optimal design principles and Wolff's law within a proper range.
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http://dx.doi.org/10.2174/1573397118666220401104428DOI Listing
April 2022

Induction of developmental toxicity and cardiotoxicity in zebrafish embryos by Emamectin benzoate through oxidative stress.

Sci Total Environ 2022 Jun 20;825:154040. Epub 2022 Feb 20.

Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Emamectin benzoate (EMB) is a widely used pesticide in agriculture, but its potential risks to the environment and health have not been fully evaluated. In this study, we evaluated the toxicity of Emamectin benzoate using zebrafish model, and found that it affected early embryonic development, such as malformations and delayed hatching. Mechanistically, Emamectin benzoate increased oxidative stress by excessive production of reactive oxygen species (ROS) and abnormal activities of the antioxidant enzymes. Moreover, Emamectin benzoate exposure caused abnormalities in zebrafish heart morphology and function, such as long SV-BA distance and slow heart rate. Alterations were induced in the transcription of heart development-related genes (nkx2.5, tbx5, gata4 and myl7). In summary, our data showed that Emamectin benzoate induces developmental toxicity and cardiotoxicity in zebrafish. Our research provides new evidence on the Emamectin benzoate's toxicity and potential risk in human health.
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http://dx.doi.org/10.1016/j.scitotenv.2022.154040DOI Listing
June 2022

MiR-1260b protects against LPS-induced degenerative changes in nucleus pulposus cells through targeting TCF7L2.

Hum Cell 2022 May 14;35(3):779-791. Epub 2022 Feb 14.

Department of Orthopaedics, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.

Nucleus pulposus (NP) cells play a critical role in maintaining intervertebral disc integrity through producing the components of extracellular matrix (ECM). NP cell dysfunction, including senescence and hyper-apoptosis, has been regarded as critical events during intervertebral disc degeneration development. In the present study, we found that Transcription Factor 7-Like 2 (TCF7L2) was overexpressed within degenerative intervertebral disc tissue samples, and TCF7L2 silencing improved lipopolysaccharide (LPS)-induced repression on NP cell proliferation, ECM synthesis, and LPS-induced NP cell senescence. miR-1260b directly targeted TCF7L2 and inhibited TCF7L2 expression. miR-1260b overexpression improved LPS-induced degenerative changes in NP cells; more importantly, TCF7L2 overexpression significantly reversed the effects of miR-1260b overexpression on LPS-stimulated degenerative changes within NP cells. For the first time, we demonstrated the function of the miR-1260b/TCF7L2 axis on the phenotypic maintenance of chondrocyte-like NP cells and ECM synthesis by NP cells under LPS stimulation.
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http://dx.doi.org/10.1007/s13577-021-00655-4DOI Listing
May 2022

A Strategy for Simultaneously Improving Resolution and Sensitivity of Hybrid Quadrupole Ion Trap/Time-of-Flight Mass Spectrometry Using Square Waveform Phase Modulation.

J Am Soc Mass Spectrom 2022 Feb 30;33(2):322-327. Epub 2021 Dec 30.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

The hybrid quadrupole ion trap/time-of-flight mass spectrometry (QIT/TOFMS) device is popular because of its advantages of high sensitivity, high resolution, and MS/MS capability. However, the analytical performance of QIT/TOFMS is severely limited by the parameters of the ion trap, as QIT is typically used as a TOF pulser because the ion initial distributions of space, velocity, and angle change dynamically with the phase angle of rf voltage. In this work, a square waveform phase modulation strategy was proposed to eliminate the influence of the rf phase angle, and the dependence of QIT/TOFMS performance on the phase angle was studied. It was found that the mass resolution and signal intensity showed a "W" trend with the increase of the ion extraction phase angle from 0° to 360°, where the best resolution and sensitivity were obtained at 0°, 180°, and 360° while the worst resolution and sensitivity were obtained at 90° and 270°. Moreover, the optimum phase angle was independent of /. As a result, the mass resolution for / 106, 164, and 258 was improved by 162%, 160%, and 210% respectively, while the signal intensities for / 106, 164, and 258 were enhanced by 25 ± 1, 10 ± 1, and 21 ± 1 fold, respectively, and a limit of detection down to 0.015 ppbv for / 164 was obtained. The experiment results indicated that the square waveform phase modulation strategy could be used to simultaneously improve the resolution and sensitivity of QIT/TOFMS.
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http://dx.doi.org/10.1021/jasms.1c00314DOI Listing
February 2022

Recent Progress in Flexible Microstructural Pressure Sensors toward Human-Machine Interaction and Healthcare Applications.

Small Methods 2021 03 18;5(3):e2001041. Epub 2021 Jan 18.

Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Department of Physics, Xiamen University, Xiamen, 361005, China.

With the rapid growth of artificial intelligence, wearable electronic devices have caught intensive research interest recently. Flexible sensors, as the significant part of them, have become the focus of research. Particularly, flexible microstructural pressure sensors (FMPSs) have attracted extensive attention because of their controllable shape, small size, and high sensitivity. Microstructures are of great significance to improve the sensitivity and response time of FMPSs. The FMPSs present great application prospects in medical health, human-machine interaction, electronic products, and so on. In this review, a series of microstructures (e.g., wave, pillar, and pyramid shapes) which have been elaborately designed to effectively enhance the sensing performance of FMPSs are introduced in detail. Various fabrication strategies of these FMPSs are comprehensively summarized, including template (e.g., silica, anodic aluminum oxide, and bionic patterns), pre-stressing, and magnetic field regulation methods. In addition, the materials (e.g., carbon, polymer, and piezoelectric materials) used to prepare FMPSs are also discussed. Moreover, the potential applications of FMPSs in human-machine interaction and healthcare fields are emphasized as well. Finally, the advantages and latest development of FMPSs are further highlighted, and the challenges and potential prospects of high-performance FMPSs are outlined.
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http://dx.doi.org/10.1002/smtd.202001041DOI Listing
March 2021

Steroid-Induced Osteonecrosis of the Femoral Head: Novel Insight Into the Roles of Bone Endothelial Cells in Pathogenesis and Treatment.

Front Cell Dev Biol 2021 30;9:777697. Epub 2021 Nov 30.

Department of Orthopedics, China-Japan Friendship Hospital, Beijing, China.

Steroid-induced osteonecrosis of the femoral head (SONFH) is a disease characterized by the collapse of the femoral head. SONFH occurs due to the overuse of glucocorticoids (GCs) in patients with immune-related diseases. Among various pathogenesis proposed, the mechanism related to impaired blood vessels is gradually becoming the most convincing hypothesis. Bone endothelial cells including bone microvascular endothelial cells (BMECs) and endothelial progenitor cells (EPCs) play a crucial role in the maintenance of vascular homeostasis. Therefore, bone endothelial cells are key regulators in the occurrence and progression of SONFH. Impaired angiogenesis, abnormal apoptosis, thrombosis and fat embolism caused by the dysfunctions of bone endothelial cells are considered to be the pathogenesis of SONFH. In addition, even with high disability rates, SONFH lacks effective therapeutic approach. Icariin (ICA, a flavonoid extracted from Epimedii Herba), pravastatin, and VO-OHpic (a potent inhibitor of PTEN) are candidate reagents to prevent and treat SONFH through improving above pathological processes. However, these reagents are still in the preclinical stage and will not be widely used temporarily. In this case, bone tissue engineering represented by co-transplantation of bone endothelial cells and bone marrow mesenchymal stem cells (BMSCs) may be another feasible therapeutic strategy.
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http://dx.doi.org/10.3389/fcell.2021.777697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670327PMC
November 2021

Free-Standing, Flexible [email protected] Films with Cross-Linked Mesoporous Structures toward Supercapacitors and Pressure Sensors.

ACS Appl Mater Interfaces 2021 Dec 29;13(48):57576-57587. Epub 2021 Nov 29.

Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Department of Physics, Xiamen University, Xiamen 361005, China.

The preparation of multifunctional materials with low cost and simple synthesis processes is still challenging. Herein, by employing various sizes (50-500 nm) of polystyrene (PS) spheres as templates, different free-standing [email protected] films with three-dimensional (3D) mesoporous structures were fabricated through a simple multistep route. The microstructure, composition, mechanical property, conductivity, electrochemical activity, and sensing characteristics of these [email protected] films were investigated in detail. The intercalation of the PS spheres can effectively reduce the self-accumulation of MXene nanosheets and construct 3D cross-linked mesoporous structures, therefore broadening the ion transport channels and exposing more active sites of [email protected] films. When applied in a symmetrical supercapacitor, the optimized [email protected] electrode has a satisfactory specific capacitance of 447.67 F g at a current density of 1 A g. Moreover, the 3D mesoporous structures of [email protected] films can significantly improve the sensitivity of the resultant pressure sensors with excellent stability (10,000 cycles). Thus, such mesoporous [email protected] films prepared by a facile yet robust route will be a versatile material for many applications.
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http://dx.doi.org/10.1021/acsami.1c16589DOI Listing
December 2021

Triboionization in Discontinuous Atmospheric Pressure Inlet for a Miniature Ion Trap Mass Spectrometer.

Anal Chem 2021 12 24;93(48):15897-15904. Epub 2021 Nov 24.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, People's Republic of China.

Discontinuous atmospheric pressure interface (DAPI) consisting of a pinch valve, a silicone tube, and two metal capillaries has been widely used in miniature mass spectrometry. It is interesting that clear ion signals could be observed even when the extra ionization source was turned off. In-depth analysis suggested that this new ionization phenomenon known as triboionization is based on the surface friction on the inner surface of the silicone tube during the on/off of the pinch valve. In this study, triboionization in the DAPI of a miniature ion trap mass spectrometer was investigated. It was discovered that the signal intensity depended greatly on the material and the roughness of the silicone tube used in the DAPI. By rubbing the inner surface of the silicone tube, for example, the signal intensity can increase by nearly 20 times. Two connected pinch valves were developed to study the effects of the discharge pressure, the number, and the frequency of on/off of the pinch valve on triboionization, which were verified to have a large impact on the product ions. In addition, the humidity of the inner surface of the silicone tube impacted the signal intensity of product ions and the mass spectrum patterns, where the product ions were typically protonated ions. As the humidity increases, the signal intensity of analytes with high proton affinity increases accordingly. This triboionization source, which does not require heat, light, radiation, auxiliary gas, or solution, has been preliminarily proved to have potential for surface detection after continuous enrichment.
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http://dx.doi.org/10.1021/acs.analchem.1c02611DOI Listing
December 2021

[Progress of developmental mechanism of subtype H vessels in osteonecrosis of the femoral head].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Nov;35(11):1486-1491

Department of Orthopedic Surgery, China-Japan Friendship Hospital, Beijing, 100029, P.R.China.

Objective: To review the research progress of subtype H vessels in the occurrence and development of osteonecrosis of the femoral head (ONFH).

Methods: The relevant domestic and foreign literature was extensively reviewed. The histological features, biological mechanism of subtype H vessels involved in promoting of osteogenesis, and the role and application of the subtype H vessels in ONFH were summarized.

Results: The subtype H vessel is a newly discovered bone vessel, mainly distributed in metaphysis and subperiosteum, highly expressing endomucin and CD31. The subtype H vessel has a dense arrangement of Runx2 early osteoprogenitors, collagen type Ⅰα osteoblast cells, and Osterix osteoprogenitors that have the ability to induce osteogenesis and angiogenesis. Factors such as platelet-derived growth factor BB, slit guidance ligand 3, hypoxia inducible factor 1α, Notch signaling pathway, and vascular endothelial growth factor are involved in the mechanism of subtype H vessels in promoting osteogenesis.

Conclusion: Subtype H vessels play an important role in the regulation of angiogenesis and osteogenesis during bone tissue repair and reconstruction. The discovery of subtype H vessels provides new insights into the molecular and cellular mechanisms of osteogenesis and angiogenesis coupling. In the future, new techniques targeting the regulation of subtype H blood vessels may become a promising method for the treatment of ONFH.
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http://dx.doi.org/10.7507/1002-1892.202103159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586765PMC
November 2021

Clinicopathological Characteristics and Survival Outcomes of Primary Renal Leiomyosarcoma.

Front Surg 2021 21;8:704221. Epub 2021 Oct 21.

Department of Urology, Xiangya Changde Hospital, Changde, China.

Primary renal leiomyosarcoma (LMS) is an exceedingly rare entity with a poor prognosis. We summarized the clinicopathological characteristics, treatment choice, and survival outcomes of LMS from the Surveillance, Epidemiology, and End Results (SEER) database. Renal LMS and kidney renal clear cell carcinoma (KIRC) data from 1998 to 2016 were collected from the SEER database. The continuous variables were analyzed using -tests, while the categorical variables were analyzed using Pearson's chi-squared or Fisher's exact tests. Propensity score matching (PSM) was also performed. The cancer-specific survival (CSS) and overall survival (OS) curves were estimated using Kaplan-Meier analyses and compared by log-rank tests. The risk factors for CSS and OS were estimated using univariable and multivariable Cox proportional hazard regression models. A total of 140 patients with renal LMS and 75,401 patients with KIRC were enrolled. These groups differed significantly in sex, race, tumor size, grade, SEER stage, surgery, radiation, and chemotherapy. Renal LMS exhibited poorer CSS and OS compared with KIRC before and after PSM. For renal LMS, the univariate Cox proportional hazard regression model indicated that larger tumor size, higher tumor grade, higher SEER stage, and chemotherapy were risk factors for CSS and OS, while surgery appeared to be a protective factor. However, only tumor grade, SEER stage, and receiving surgery remained independent prognostic factors in the multivariable Cox proportional hazard regression model. In addition, subgroup analyses indicated that surgery remained a protective factor for advanced renal LMS. However, there was no survival benefit for patients receiving chemotherapy. Primary renal LMS is an exceedingly rare entity with distinct clinicopathological features and a poor prognosis. A higher tumor grade and late stage may indicate a poor prognosis. Complete tumor resection remains to be the first treatment choice, while chemotherapy may be a palliative treatment for patients with advanced disease.
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http://dx.doi.org/10.3389/fsurg.2021.704221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566676PMC
October 2021

Segmentation and Automatic Identification of Vasculature in Coronary Angiograms.

Comput Math Methods Med 2021 7;2021:2747274. Epub 2021 Oct 7.

Department of Cardiology, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 758 Hefei Road, Qingdao, Shandong 266035, China.

Coronary angiography is the "gold standard" for the diagnosis of coronary heart disease, of which vessel segmentation and identification technologies are paid much attention to. However, because of the characteristics of coronary angiograms, such as the complex and variable morphology of coronary artery structure and the noise caused by various factors, there are many difficulties in these studies. To conquer these problems, we design a preprocessing scheme including block-matching and 3D filtering, unsharp masking, contrast-limited adaptive histogram equalization, and multiscale image enhancement to improve the quality of the image and enhance the vascular structure. To achieve vessel segmentation, we use the C-V model to extract the vascular contour. Finally, we propose an improved adaptive tracking algorithm to realize automatic identification of the vascular skeleton. According to our experiments, the vascular structures can be successfully highlighted and the background is restrained by the preprocessing scheme, the continuous contour of the vessel is extracted accurately by the C-V model, and it is verified that the proposed tracking method has higher accuracy and stronger robustness compared with the existing adaptive tracking method.
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http://dx.doi.org/10.1155/2021/2747274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516542PMC
January 2022

Multiple osteolytic primary peripheral T-cell bone lymphoma: the first case report.

J Int Med Res 2021 Oct;49(10):3000605211052229

Department of Hematology, Fuyang People's Hospital (The Affiliated Fuyang People's Hospital of Anhui Medical University), Fuyang City, Anhui Province, China.

Peripheral T-cell lymphoma accounts for about 10% of all cases of non-Hodgkin's lymphoma. However, less than 5% of patients with non-Hodgkin's lymphoma present with hypercalcaemia as the initial symptom, and less than 1% present with primary bone lesions. We herein describe a 76-year-old Chinese man who was diagnosed with primary bone adult T-cell lymphoma with extensive osteolysis, including bone loss in the radius, as the initial manifestation. He had developed severe generalised bone pain and an inability to raise his arms. X-ray examination revealed osteolytic destruction of the forearm with loss of the radial diaphysis. The patient was diagnosed with peripheral T-cell lymphoma based on his immunohistochemical results. He began treatment with the CHOPE chemotherapy regimen, which resulted in significant improvement of his bone pain.
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http://dx.doi.org/10.1177/03000605211052229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524712PMC
October 2021

Parallel Coupling of Ion Mobility Spectrometry and Ion Trap Mass Spectrometry for the Real-Time Alarm Triggering and Identification of Hazardous Chemical Leakages.

Anal Chem 2021 08 18;93(34):11852-11858. Epub 2021 Aug 18.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian Institute of Chemical Physics, Dalian 116023, China.

Hazardous chemical leakages involved in chemical terrorist attacks and chemical industrial accidents have been posing severe threats to human health and the environment. Vehicle-mounted mass spectrometry (MS) has been developed for continuous, on-road measurements to map the spatial and temporal distributions of hazardous chemicals. However, the detection of chemicals with small temporal scales and spatial scales is always challenging. In this study, a parallel coupling apparatus combining the techniques of ion mobility spectrometry and ion trap MS (-IMS-ITMS) was developed to improve the detection rate and the time response capability of a stand-alone ITMS system for short time-span chemical tracking. A workflow was also proposed along with the apparatus, where the ITMS system can be triggered, as chemical suspects were discovered with the IMS system. The sampling positions of the ITMS system were investigated and optimized. In addition, a strategy was proposed to diminish the time span of samples from 1.5 to 0.5 s for evaluating the performances of the -IMS-ITMS system. The detection rate of the stand-alone ITMS system was measured to be only 9.5, 32, and 87.5% for the time span of 0.5, 1, and 1.5 s, respectively. By comparison, the detection rates of the -IMS-ITMS system were 99.5, 100, and 100%, where the detection rate was increased by a factor of 10 for 0.5 s time span. Moreover, the addition of an IMS system could provide temporal patterns of hazardous chemicals with a resolution of 33 ms. Finally, the potential of the -IMS-ITMS system for environmental navigation monitoring and assessment was further demonstrated by detecting the leakages of dimethyl methyl phosphonate and dipropylene glycol monomethyl ether.
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http://dx.doi.org/10.1021/acs.analchem.1c02647DOI Listing
August 2021

Cannulated screw fixation versus plate fixation in treating displaced intra-articular calcaneus fractures: a systematic review and meta-analysis.

Int Orthop 2021 09 9;45(9):2411-2421. Epub 2021 Aug 9.

Department of Orthopaedic Surgery, China-Japan Friendship Hospital, No. 2 Yinghua East Road, Chaoyang District, Beijing, 100029, China.

Background: Operative treatment has become the current trend for displaced intra-articular calcaneus fracture (DIACF), while using cannulated screw fixation or plate fixation is still controversial for treating DIACF. The purpose of this meta-analysis is to compare the outcome of the two fixation methods.

Methods: We searched literature comparing cannulated screw fixation and plate fixation from PubMed, Embase, Web of Science, and Cochrane Library. Only randomized controlled trials were included. The outcomes of post-operative function, radiological measurement, time efficiency, and wound complications were pooled in the meta-analysis.

Result: Seven RCTs with 902 cases of DIACF were included. Pooled results showed the two fixation methods that had similar function satisfactory of AOFAS score (RR = 0.95, 95% CI = [0.83, 1.09], P = 0.47, I = 0%) and Maryland Foot score (RR = 0.93, 95% CI = [0.68, 1.28], P = 0.66, I = 84%). Compared to plate fixation, cannulated screw fixation had better improvement of Bohler's angle (WMD = 0.56, 95% CI = [0.20, 0.91], P = 0.002, I = 34%) and Gissane's angle (WMD = 1.36, 95% CI = [0.56, 2.16], P = 0.0008, I = 7%), better recovery of calcaneal height (WMD = 0.49, 95% CI = [0.02, 0.95], P = 0.04, I = 6%), shorter time to operation (WMD =  - 2.91, 95% CI = [- 4.99, - 0.84], P = 0.006, I = 97%), less operation time (WMD =  - 21.58, 95% CI = [- 37.31, - 5.85], P = 0.007, I = 98%), reduced length of hospital stay (WMD =  - 2.00, 95% CI = [- 3.69, - 0.31], P = 0.02, I = 97%), and less wound complications (RR = 0.16, 95% CI = [0.08, 0.32], P < 0.00001, I = 0%).

Conclusion: Cannulated screw fixation and plate fixation have similar postoperative functional satisfactory. Cannulated screw fixation is superior to plate fixation in reduction quality, time efficiency, and wound complications.
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http://dx.doi.org/10.1007/s00264-021-05141-yDOI Listing
September 2021

Sensor and machine learning-based assessment of gap balancing in cadaveric unicompartmental knee arthroplasty surgical training.

Int Orthop 2021 11 5;45(11):2843-2849. Epub 2021 Aug 5.

Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Purpose: The aim of this study was to assess the difference between flexion and extension contact forces-gap balance-after Oxford mobile-bearing medial unicompartmental knee arthroplasty (UKA) performed by surgeons with varying levels of experience.

Methods: Surgeons in a training programme performed UKAs on fresh frozen cadaveric specimens (n = 60). Contact force in the medial compartment of the knee was measured after UKA during extension and flexion using a force sensor, and values were clustered using an unsupervised machine learning (k-means algorithm). Univariate analysis was performed with general linear regression models to identify the explanatory variable.

Results: The level of experience was predictive of gap balance; surgeons were clustered into beginner, mid-level and experienced groups. Experienced surgeons' mean difference between flexion and extension contact force was 83 N, which was significantly lower (p < 0.05) than that achieved by mid-level (215 N) or beginner (346 N) surgeons.

Conclusion: We found that the lowest mean difference between flexion and extension contact force after UKA was 83 N, which was achieved by surgeons with the most experience; this value can be considered the optimal value. Beginner and mid-level surgeons achieved values that were significantly lower. This study also demonstrates that machine learning can be used in combination with sensor technology for improving gap balancing judgement in UKA.
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http://dx.doi.org/10.1007/s00264-021-05176-1DOI Listing
November 2021

Patient-specific instrumentation (PSI) in total ankle arthroplasty: a systematic review.

Int Orthop 2021 09 4;45(9):2445-2452. Epub 2021 Aug 4.

Department of Orthopaedic Surgery, China-Japan Friendship Hospital, No. 2 Yinghua East Road, Chaoyang District, Beijing, 100029, China.

Background: Patient-specific instrument (PSI) may theoretically make total ankle arthroplasty (TAA) more accurate. Several studies have reported the outcomes of PSI TAA. The aim of this study is to systematically review the literature of PSI TAA.

Methods: PubMed, Embase, Web of Science, and Cochrane Library databases were systematically reviewed according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines for PSI TAA. The quality of the included studies was evaluated according to Methodological Index for Non-Randomized Studies (MINORS).

Result: Nine articles were ultimately included in the systematic review. The implant position and function outcome of TAA was similar between PSI and SI. Prediction accuracy of implant size remained great difference. PSI can shorten the operative time and fluoroscopy time. The quality of current studies on PSI TAA is insufficient to produce high-level evidence.

Conclusion: PSI can get similar implant position and clinical outcome in TAA compared to SI, but current evidence is not strong enough to evaluate PSI TAA.
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http://dx.doi.org/10.1007/s00264-021-05145-8DOI Listing
September 2021

Efficacy and Safety of Intra-Articular Platelet-Rich Plasma in Osteoarthritis Knee: A Systematic Review and Meta-Analysis.

Biomed Res Int 2021 30;2021:2191926. Epub 2021 Apr 30.

Department of Orthopaedic Surgery, Beijing Key Lab Immune-Mediated Inflammatory Diseases, China-Japan Friendship Hospital, Beijing 100029, China.

Background: Knee osteoarthritis (KOA) is a common disease in aged adults. Intra-articular (IA) injection of platelet-rich plasma (PRP) therapy is an effective minimally invasive treatment for KOA. We aimed to compare the efficacy and safety of platelet-rich plasma (PRP) with placebo or other conservative treatments.

Methods: We conducted a meta-analysis to identify relevant articles from online register databases such as PubMed, Medline, Embase, and the Cochrane Library. The primary outcomes were the visual analogue scale (VAS) score, Western Ontario and McMaster Universities Arthritis Index (WOMAC) score, and International Knee Documentation Committee (IKDC) subjective score. The secondary outcome was the adverse event rate.

Results: A total of 895 articles were identified, of which 23 randomized controlled trials that met the inclusion criteria were determined as eligible. Compared with placebo, PRP had a lower VAS score and higher IKDC subjective score at the 6 month after treatment and significantly less WOMAC score during the follow-up period. Compared with oral NSAIDs, PRP gained a lower WOMAC score at the 6 month after treatment. The VAS score decreased after treatment when reaching PRP and CS. As compared to the HA, the VAS score, WOMAC score, and IKDC subjective score all revealed better PRP results. There were no significant differences in adverse event rates comparing PRP versus placebo or HA. Different PRP applications did not show significant differences in VAS score in the 1 month and WOMAC score in the 3 month after treatment.

Conclusion: To compare with the conservative treatments mentioned above, PRP is more effective in relieving symptoms. There were no significant differences between triple PRP application and single PRP application in short-term curative effect.
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http://dx.doi.org/10.1155/2021/2191926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294028PMC
September 2021

Autologous Osteochondral Transplantation for Young Patients with Postcollapse Osteonecrosis of the Knee: A Retrospective Cohort Study with an Average 7-Year Follow-Up.

Cartilage 2021 12 17;13(1_suppl):1291S-1297S. Epub 2021 Jun 17.

Department of Orthopaedic Surgery, Center for Osteonecrosis and Joint Preserving & Reconstruction, China-Japan Friendship Hospital, Beijing, China.

Background: Only a few studies exist that have assessed the efficacy of autologous osteochondral transplantation (AOT) treatment of osteonecrosis (ON) of the knee. The purpose of this study was to clarify the clinical and radiographic results of AOT performed on young patients with postcollapse ON of the knee.

Methods: This retrospective study included 14 young patients (6 men, 8 women, mean age 34.71 ± 5.41 years) with stage III knee ON undergoing AOT surgery. Mean follow-up time was 87 ± 10.23 months. The postoperative clinical and radiological evaluations were based on Hospital for Special Surgery (HSS) scores and plain x-rays. Paired tests were used for the statistical analysis by SPSS software.

Results: Preoperative HSS (mean 66.86 ± 7.49 points) was significantly improved at mean 7 years to a postoperative HSS (mean 87.36 ± 8.63 points) ( < 0.001). The postoperative rangeof motion (ROM; 125.71° ± 4.74°) was not significantly different from the preoperative ROM (126.86° ± 7.13°) ( = 0.626). There was no progression of collapse observed in any knees at the last follow-up. No knees required any reoperations during the follow-up period.

Conclusion: Fourteen young patients with local ON of the knee were treated by AOT. No progression of bone collapse was seen and clinical improvement sustained at mean 7 years postsurgery. AOT seems to be a safe technique that can give long-term improvement and durability in young ON patients. However, large-sample and high-quality clinical trials are still needed in the future.
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http://dx.doi.org/10.1177/19476035211023566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808810PMC
December 2021

Real-time continuous measurement of intraoperative trace exhaled propofol by planar differential mobility spectrometry.

Anal Methods 2021 06 25;13(23):2624-2630. Epub 2021 May 25.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, People's Republic of China.

In order to study anesthetic pharmacokinetics and adequately adjust the anaesthesia depth of patients, real-time measurement of the intraoperative exhaled propofol concentration is of significant importance for anaesthetists. Although a series of analytical techniques and methods have been developed for the detection of exhaled propofol, differential mobility spectrometry (DMS) with the advantages of a much smaller instrument, faster response time and cheaper cost shows great potential for the point of care in the operating room. In this paper, a planar DMS was constructed for real-time continuous measurement of trace propofol in exhaled air. The effects of DMS parameters, such as the radio frequency voltage, the drift gas flow rate and the sampling flow rate of exhaled air on the propofol measurement under high humidity conditions were carefully investigated and discussed. Under the optimum experimental conditions, the limit of detection (LOD) for propofol was achieved in ppbv with a linear range of 0.5 to 25 ppbv, both of which meet clinical requirements. Finally, the planar DMS was performed on a patient undergoing thyroidectomy surgery to real-time monitor the intraoperative exhaled propofol, which demonstrated the capability of DMS for sensitive and breath-by-breath continuous measurement of intraoperative trace exhaled propofol.
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http://dx.doi.org/10.1039/d1ay00179eDOI Listing
June 2021
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