Publications by authors named "Weidong Liu"

274 Publications

Prognostic Value of Blood Pressure Variability for Patients With Acute or Subacute Intracerebral Hemorrhage: A Meta-Analysis of Prospective Studies.

Front Neurol 2021 11;12:606594. Epub 2021 Mar 11.

Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, China.

The results on the role of systolic blood pressure (SBP) variability in the functional outcome for patients with intracerebral hemorrhage (ICH) have been inconsistent. Hence, this meta-analysis of prospective studies was conducted to assess the association between SBP variability and poor outcomes in patients with acute or subacute ICH. PubMed, Embase, and the Cochrane Library were electronically searched for eligible studies from their inception to July 2020. The role of SBP variability assessed using standard deviation (SD), coefficient of variation (CV), successive variation (SV), average real variability (ARV), and residual standard deviation (RSD) in the risk of poor functional outcomes were assessed using odds ratio (OR) with 95% confidence interval (CI) through the random-effects model. Seven prospective studies involving 5,201 patients with ICH were selected for the final meta-analysis. Increased SBP variability was associated with an increased risk of poor functional outcomes, regardless of its assessment using SD (OR: 1.38; 95% CI: 1.14-1.68; = 0.001), CV (OR: 1.98; 95% CI: 1.13-3.47; = 0.017), SV (OR: 1.30; 95% CI: 1.08-1.58; = 0.006), ARV (OR: 1.13; 95% CI: 1.03-1.24; = 0.010), or RSD (OR: 1.22; 95% CI: 1.00-1.50; = 0.049). Moreover, the role of SBP variability in the risk of poor functional outcomes for patients with ICH was affected by country, study design, mean age, stroke type, outcome definition, and study quality. This study indicated that SBP variability was a predictor of poor outcomes for patients with ICH.
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http://dx.doi.org/10.3389/fneur.2021.606594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991598PMC
March 2021

Crucial roles of different RNA-binding hnRNP proteins in Stem Cells.

Int J Biol Sci 2021 8;17(3):807-817. Epub 2021 Feb 8.

Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha 410008, China.

The self-renewal, pluripotency and differentiation of stem cells are regulated by various genetic and epigenetic factors. As a kind of RNA binding protein (RBP), the heterogeneous nuclear ribonucleoproteins (hnRNPs) can act as "RNA scaffold" and recruit mRNA, lncRNA, microRNA and circRNA to affect mRNA splicing and processing, regulate gene transcription and post-transcriptional translation, change genome structure, and ultimately play crucial roles in the biological processes of cells. Recent researches have demonstrated that hnRNPs are irreplaceable for self-renewal and differentiation of stem cells. hnRNPs function in stem cells by multiple mechanisms, which include regulating mRNA stability, inducing alternative splicing of mRNA, epigenetically regulate gene expression, and maintaining telomerase activity and telomere length. The functions and the underlying mechanisms of hnRNPs in stem cells deserve further investigation.
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http://dx.doi.org/10.7150/ijbs.55120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975692PMC
February 2021

YBX1 is required for maintaining myeloid leukemia cell survival by regulating BCL2 stability in an m6A-dependent manner.

Blood 2021 Mar 24. Epub 2021 Mar 24.

Wuhan University, Wuhan, China.

RNA-binding proteins (RBPs) are critical regulators of transcription and translation that are often dysregulated in cancer. Although RBPs are increasingly appreciated as being important for normal hematopoiesis and for hematological malignancies as oncogenes or tumor suppressors, essential RBPs for leukemia maintenance and survival remain elusive. Here we show that YBX1 is specifically required for maintaining myeloid leukemia cell survival in an m6A-dependent manner. We found that expression of YBX1 is significantly upregulated in myeloid leukemia cells, and deletion of YBX1 dramatically induces apoptosis, promotes differentiation, coupled with reduced proliferation and impaired leukemic capacity of primary human and mouse acute myeloid leukemia (AML) cells in vitro and in vivo. Loss of YBX1 does not obviously affect normal hematopoiesis. Mechanistically, YBX1 interacts with IGF2BPs and stabilizes m6A-tagged RNA. Moreover, YBX1 deficiency dysregulates the expression of apoptosis-related genes, and promotes mRNA decay of MYC and BCL2 in an m6A-dependent manner, which contributes to the defective survival due to YBX1 deletion. Thus, our findings uncover a selective and critical role of YBX1 in maintaining myeloid leukemia survival that might provide a rationale for the therapeutic targeting of YBX1 in myeloid leukemia.
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http://dx.doi.org/10.1182/blood.2020009676DOI Listing
March 2021

MiR-129-5p Suppresses Cell Proliferation of Human Osteosarcoma Cancer by Down-Regulating LncRNA Lnc712.

Cancer Manag Res 2021 10;13:2259-2264. Epub 2021 Mar 10.

Department of Orthopaedics, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an City, Nanjing Province, 223300, People's Republic of China.

Introduction: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).

Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay.

Results: Lnc712 was upregulated in OS and was inversely correlated with miR-129-5p. In OS cells, Lnc712 overexpression failed to significantly affect miR-129-5p, while miR-129-5p overexpression led to downregulated Lnc712. Cell proliferation showed that Lnc712 overexpression resulted in increased cell proliferation rate. MiR-129-5p overexpression played an opposite role and reversed the effect of Lnc712 overexpression.

Discussion: MiR-129-5p may suppress cell proliferation of OS by down-regulating Lnc712.
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http://dx.doi.org/10.2147/CMAR.S284078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956586PMC
March 2021

Clinical Correction of Complete Median Cleft of the Mandible and Lower Lip: A 17-Year Follow-Up of a Case Report With Literature Review.

Cleft Palate Craniofac J 2021 Mar 8:1055665621990170. Epub 2021 Mar 8.

Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

The median cleft of the mandible and lower lip is an extremely rare congenital maxillofacial deformity, and the therapeutic options are controversial. To evaluate the clinical characteristics and identify a better choice of treatment modes used among us and others, we reviewed 34 relevant literature and herein describe a 17-year follow-up of a case with a median cleft of the mandible and lower lip. Based on the literature and our case with good functional and aesthetical outcomes, we propose a prospective clinical treatment: Patients of Tessier 30 cleft associated with cleft of the mandible could undergo mandibular repair after puberty in conditions of a good occlusal relationship and normal maxillofacial development, even with mild masticatory dysfunction.
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http://dx.doi.org/10.1177/1055665621990170DOI Listing
March 2021

S1PR2 Inhibition Attenuates Allergic Asthma Possibly by Regulating Autophagy.

Front Pharmacol 2020 10;11:598007. Epub 2021 Feb 10.

Jilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji, China.

This study is to investigate the role of Sphingosine-1-phosphate (S1P) in the asthma progression, and the involvement of autophagy. Airway remodeling mice were subjected to the HE, PAS, and Masson staining. Protein expression levels in the tissues, samples and model cells were detected with ELISA, Western blot analysis, and immunohistochemical/immunofluorescent analysis. The S1P2 receptor antagonist JTE-013 decreased the inflammatory cell infiltration and goblet cell production in asthmatic mice tissues. The IL-1, IL-4, IL-5 and serum IgE contents were decreased in bronchoalveolar lavage fluid, while the Beclin1 expression in lung tissues was decreased. The LC3B1 to LC-3B2 conversion was decreased, with increased P62 accumulation and decreased p-P62 expression. In airway remodeling mice, JTE-013 significantly decreased collagen deposition in lung tissues and decreased smooth muscle cell smooth muscle activating protein expression. In lung tissue, the expression levels of Beclin1 were decreased, with decreased LC3B1 to LC-3B2 conversion, as well as the increased P62 accumulation and decreased p-P62 expression. However, these effects were reversed by the RAC1 inhibitor EHT 1864. Similar results were observed for the silencing of S1P2 receptor in the cells, as shown by the decreased Beclin1 expression, decreased LC3B1 to LC-3B2 conversion, increased P62 accumulation, and decreased p-P62 expression. The smooth muscle activators were significantly decreased in the JTE-013 and EHT1864 groups, and the EHT 1864 + S1P2-SiRNA expression level was increased. S1P is involved in the progression of asthma and airway remodeling, which may be related to the activation of S1PR2 receptor and inhibition of autophagy through RAC1.
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http://dx.doi.org/10.3389/fphar.2020.598007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902893PMC
February 2021

Crystal Structures and Catalytic Mechanism of l-erythro-3,5-Diaminohexanoate Dehydrogenase and Rational Engineering for Asymmetric Synthesis of β-Amino Acids.

Angew Chem Int Ed Engl 2021 Feb 24. Epub 2021 Feb 24.

National Engineering Laboratory for Industrial Enzymes and Tianjin Engineering Research Center of Biocatalytic Technology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, and, National Technology Innovation Center for Synthetic Biology, Tianjin, 300308, China.

Amino acid dehydrogenases (AADHs) have shown considerable potential as biocatalysts in the asymmetric synthesis of chiral amino acids. However, compared to the widely studied α-AADHs, limited knowledge is available about β-AADHs that enable the synthesis of β-amino acids. Herein, we report the crystal structures of a l-erythro-3,5-diaminohexanoate dehydrogenase and its variants, the only known member of β-AADH family. Crystal structure analysis, site-directed mutagenesis studies and quantum chemical calculations revealed the differences in the substrate binding and catalytic mechanism from α-AADHs. A number of rationally engineered variants were then obtained with improved activity (by 110-800 times) toward various aliphatic β-amino acids without an enantioselectivity trade-off. Two β-amino acids were prepared by using the outstanding variants with excellent enantioselectivity (>99 % ee) and high isolated yields (86-87 %). These results provide important insights into the molecular mechanism of 3,5-DAHDH, and establish a solid foundation for further design of β-AADHs for the asymmetric synthesis of β-amino acids.
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http://dx.doi.org/10.1002/anie.202017225DOI Listing
February 2021

A retrospective study assessing the acceleration effect of type I Helicobacter pylori infection on the progress of atrophic gastritis.

Sci Rep 2021 Feb 18;11(1):4143. Epub 2021 Feb 18.

Department of Gastroenterology, People's Hospital of Xinjiang Uygur Autonomous Region, No. 91 Tianchi Road, Tianshan District, Urumqi, 83000, China.

Based on the antibody typing classification, Helicobacter pylori infection can be divided into type I H. pylori infection and type II H. pylori infection. To observe the effects of different H. pylori infection types on the distribution of histopathological characteristics and the levels of three items of serum gastric function (PG I, PG II, G-17). 1175 cases from October 2018 to February 2020 were collected with ratio 1:2. All patients were performed with C-Urea breath test (C-UBT), H. pylori antibody typing classification, three items of serum gastric function detection, painless gastroscopy, pathological examination, etc. According to H. pylori antibody typing classification, patients were divided into three groups: type I H. pylori infection group, type II H. pylori infection group and control group. Significant difference existed among type I H. pylori infection group, type II H. pylori infection group and control group in inflammation and activity (χ = 165.43, 354.88, P all < 0.01). The proportion of three groups in OLGA staging had statistic difference (χ = 67.99, P all < 0.01); Compared with type II H. pylori infection group and control group, the level of pepsinogen I, pepsinogen II, gastrin17 in type I H. pylori infection group increased, and PG I/PG II ratio (PG I/PG II ratio, PGR) decreased, which was statistically significant (χ = 35.08, 166.24, 134.21, 141.19; P all < 0.01). Type I H. pylori infection worsened the severity of gastric mucosal inflammation and activity. H. pylori infection was prone to induce atrophy of gastric mucosa, while type I H. pylori infection played a key role in promoting the progress of atrophic gastritis and affected the level of serum gastric function. The study indicated that the eradication of H. pylori should be treated individually.
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http://dx.doi.org/10.1038/s41598-021-83647-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892840PMC
February 2021

Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis.

Methods Enzymol 2021 16;648:337-356. Epub 2021 Jan 16.

Macromolecular Crystallography, Institute of Biochemistry, University of Greifswald, Greifswald, Germany. Electronic address:

The concept of biocatalytic PET degradation for industrial recycling processes had made a big step when the bacterium Ideonella sakaiensis was discovered to break PET down to its building blocks at ambient temperature. This process involves two enzymes: cleavage of ester bonds in PET by PETase and in MHET, the resulting intermediate, by MHETase. To understand and further improve this unique capability, structural analysis of the involved enzymes was aimed at from early on. We describe a repertoire of methods to this end, including protein expression and purification, crystallization of apo and substrate-bound enzymes, and modeling of PETase complexed with a ligand.
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http://dx.doi.org/10.1016/bs.mie.2020.12.015DOI Listing
January 2021

The effect of topiramate versus flunarizine on the non-headache symptoms of migraine.

Int J Neurosci 2021 Feb 10:1-7. Epub 2021 Feb 10.

Department of Neurology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Objectives: To investigate the impact of topiramate versus flunarizine on the non-headache symptoms (NHS) of migraine, and to observe the changes of dopamine (DA) and prolactin (PRL) before and after prophylactic treatment.

Methods: Sixty-six episodic migraine patients were enrolled and randomized 1:1 to receive either flunarizine or topiramate treatment. Clinical characteristics and NHS associated with migraine were investigated before and after prophylactic treatment. The DA and PRL levels were also determined before and after prophylactic treatment.

Results: The NHS of migraine in the two groups were significantly better after treatment than before treatment in premonitory phase (PP), headache phase (HP), and resolution phase (RP). The NHS in the two groups had no significant difference in PP, HP, and RP before and after treatment. In the flunarizine group, the PRL content after treatment was significantly higher than that before treatment ( -4.097,  < 0.001), but the DA content was decreased slightly compared with that before treatment ( = 1.909,  = 0.066). There was no significant difference in PRL content ( = 1.099,  = 0.280) and DA content ( = 1.556,  = 0.130) in topiramate group before and after treatment.

Conclusions: The two classical prophylactic drugs of migraine were significantly effective in treating the NHS of migraine, but there was no significant difference between the two drugs. The DA-PRL axis may be involved in the underlying mechanism of the flunarizine treatment for the NHS of migraine.
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http://dx.doi.org/10.1080/00207454.2021.1881091DOI Listing
February 2021

Niacin Metal-Organic Framework-Laden Self-Healing Hydrogel for Wound Healing.

J Biomed Nanotechnol 2020 Dec;16(12):1719-1726

Bacterial infection is one of the most significant impediments to wound healing. To treat wounds, non-biological and non-pharmacological interventions have gained increasing importance as they are easily translatable into clinical products. In this study, we developed a niacin metal-organic frameworks (NOFs)-laden hybrid hydrogel with self-healing ability for better wound healing. The copper based-NOFs (Cu-NOFs) and zinc based-NOFs (Zn-NOFs) were constructed and encapsulated into the self-healing hydrogels, which could prevent NOFs from directly interacting with protein-containing solutions (e. g., tissue fluid). The hydrogel was prepared by using four-armed benzaldehyde-terminated polyethylene glycol (BAPEG) and carboxymethyl chitosan (CMC). Our results showed that the hydrogel gained self-healing ability through reversible Schiff base reaction. In addition, after loading NOFs, the hybrid hydrogel exerted significant effects on antibacterial and antioxidant activities. The in vivo studies demonstrated that the hybrid hydrogel could alleviate inflammation and enhance the formation of granulation tissue, collagen and vascular tissue, thereby promoting wound closure in rats with E. coli -infected wounds. Overall, this study highlights the tremendous potential for the clinical implementation of NOFs-laden hydrogel with good self-healing, antibacterial and antioxidant properties in wound healing.
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http://dx.doi.org/10.1166/jbn.2020.3000DOI Listing
December 2020

Galactosamine-modified PEG-PLA/TPGS micelles for the oral delivery of curcumin.

Int J Pharm 2021 Feb 21;595:120227. Epub 2021 Jan 21.

Department of Pharmaceutics, College of Pharmacy, Shandong University, Jinan 250012, China. Electronic address:

In this study, galactosamine-modified poly(ethylene glycol)-poly(lactide) (Gal-PEG-PLA) polymers were synthesized and Gal-PEG-PLA/D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) micelles named as GPP micelles were designed to promote the oral absorption of a hydrophobic drug, curcumin (CUR). CUR-loaded Gal-PEG-PLA/TPGS micelles (CUR@GPP micelles) were fabricated using the thin-film dispersion method. CUR@GPP micelles had a size of about 100 nm, a near-neutral zeta potential, drug loading (DL) of 14.6%, and sustained release properties. GPP micelles with high Gal density (GPP3 micelles) were superior in facilitating uptake in epithelial cells and improving intestinal permeation. In situ intestinal absorption studies suggested that the jejunum and ileum were the best absorption segments in the intestinal tract. Additionally, biodistribution results revealed that GPP3 micelles could be remarkably taken up by the jejunum and ileum. Pharmacokinetics revealed that the maximum plasma concentration (C) and the area under the plasma concentration-time curve from 0 to 24 h (AUC) for CUR@GPP3 micelles were both significantly increased, and that the relative bioavailability of CUR@GPP3 micelles to CUR-loaded mPEG-PLA/TPGS micelles (CUR@PP micelles) was 258.8%. Furthermore, CUR-loaded micelles could reduce damage to the liver and intestinal tissues. This study highlights the importance of Gal content in the design of targeting nanocarrier Gal-modified micelles, which have broad prospects for oral delivery of hydrophobic drugs. Therefore, they could serve as a promising candidate for targeted delivery to the liver.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120227DOI Listing
February 2021

Uric acid inhibits HMGB1-TLR4-NF-κB signaling to alleviate oxygen-glucose deprivation/reoxygenation injury of microglia.

Biochem Biophys Res Commun 2021 Feb 9;540:22-28. Epub 2021 Jan 9.

Department of Endocrinology, Xiang'an Hospital of Xiamen University, Xiamen, Fujian, China; Xiamen Key Laboratory of Translational Medicine for Nucleic Acid Metabolism and Regulation, Xiamen, Fujian, China. Electronic address:

Mounting evidence has implicated inflammation in ischemia-reperfusion injury following acute ischemic stroke (AIS). Microglia remain the primary initiator and participant of brain inflammation. Emerging evidence has indicated that uric acid has promise for the treatment of AIS, but its explicit mechanisms remain elusive. Here, we observed that uric acid reduced the severity of cerebral infarction and attenuated the activation of microglia in the cerebral cortex in a mouse middle cerebral-artery occlusion/reperfusion model. Thus, we speculated that uric acid may play a role by directly interfering with the inflammatory response of microglia. First, we investigated whether the HMGB1-TLR4-NF-κB signaling plays a role in oxygen glucose deprivation and reperfusion (OGD/R) injury of BV2 cells. Inhibition of the signaling significantly reduced the release of the proinflammatory cytokines tumor necrosis factor α (TNF-α), interleukin 1β (IL1β), and IL6 caused by OGD/R in BV2 cells. Second, uric acid weakened the decreased cell viability and lactate dehydrogenase release induced by OGD/R in BV2 cells. Finally, uric acid reduced the release of the proinflammatory cytokines TNF-α, IL1β, and IL6 caused by OGD/R in BV2 cells by dampening HMGB1-TLR4-NF-κB signaling, which was reversed by probenecid treatment, an inhibitor of the uric acid channel. Hence, uric acid halted the release of inflammatory factors and the decreased cell viability induced by ODG/R via inhibiting the microglia HMGB1-TLR4-NF-κB signaling, thereby alleviating the damage to microglia. This may be part of the molecular mechanisms by which uric acid protects mice against the brain damage of middle cerebral-artery occlusion/reperfusion.
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http://dx.doi.org/10.1016/j.bbrc.2020.12.097DOI Listing
February 2021

Three Immune-Associated Subtypes of Diffuse Glioma Differ in Immune Infiltration, Immune Checkpoint Molecules, and Prognosis.

Front Oncol 2020 23;10:586019. Epub 2020 Dec 23.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.

Diffuse glioma is one of the most prevalent malignancies of the brain, with high heterogeneity of tumor-infiltrating immune cells. However, immune-associated subtypes of diffuse glioma have not been determined, nor has the effect of different immune-associated subtypes on disease prognosis and immune infiltration of diffuse glioma patients. We retrieved the expression profiles of immune-related genes from The Cancer Genome Atlas (TCGA) (n = 672) and GSE16011 (n = 268) cohorts and used them to identify subtypes of diffuse glioma via Consensus Cluster Plus analysis. We used the limma, clusterProfiler, ESTIMATE, and survival packages of R for differential analysis, functional enrichment, immune and stromal score evaluation respectively in three subtypes, and performed log-rank tests in immune subtypes of diffuse glioma. The immune-associated features of diffuse glioma in the two cohorts were characterized via bioinformatic analyses of the mRNA expression data of immune-related genes. Three subtypes (C1-3) of diffuse glioma were identified from TCGA data, and were verified using the GSE16011 cohort. We then evaluated their immune characteristics and clinical features. Our mRNA profiling analyses indicated that the different subtypes of diffuse glioma presented differential expression profile of specific genes and signal pathways in the TCGA cohort. Patients with subtype C1, who were mostly diagnosed with grade IV glioma, had poorer outcomes than patients with subtype C2 or C3. Subtype C1 was characterized by a higher degree of immune cell infiltration as estimated by GSVA, and more frequent wildtype IDH1. By contrast, subtype C3 included more grade II and -mutated glioma, and was associated with more infiltration of CD4T cells. Most subtype C2 had the features between subtypes C1 and C3. Meanwhile, immune checkpoints and their ligand molecules, including PD1/(PD-L1/PDL2), CTLA4/(CD80/CD86), and B7H3/TLT2, were significantly upregulated in subtype C1 and downregulated in subtype C3. In addition, patients with subtype C1 exhibited more frequent gene mutations. Univariate and multivariate Cox regression analyses revealed that diffuse glioma subtype was an effective, independent, and better prognostic factor. Therefore, we established a novel immune-related classification of diffuse glioma, which provides potential immunotherapy targets for diffuse glioma.
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http://dx.doi.org/10.3389/fonc.2020.586019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786360PMC
December 2020

Comparison of the Characteristics and Risk Factors of Carotid Atherosclerosis in High Stroke Risk Populations Between Urban and Rural Areas in North China.

Front Neurol 2020 9;11:554778. Epub 2020 Nov 9.

Department of Neurology, Affiliated Hospital of Weifang Medical University, Weifang, China.

To study the characteristics and risk factors of carotid atherosclerosis in populations at high risk of stroke in urban and rural areas of North China. A cross-sectional study was conducted to investigate high stroke risk populations in representative urban and rural areas sampled from 12 regions of China. A pre-designed questionnaire, ultrasound, and laboratory examinations were performed to evaluate risk factors. A total of 30,175 patients were included in the study. The overall prevalence of carotid atherosclerosis was 54.53%, among which intimal thickening and plaque were 39.22 and 41.25%, respectively. The prevalence of carotid atherosclerosis in the urban group was higher than in the rural group. Multivariate logistic regression analysis revealed that male gender, age, smoking, hypertension, dyslipidemia, stroke, atrial fibrillation, systolic blood pressure, and levels of fasting blood glucose, total cholesterol, and low-density lipoprotein cholesterol were the common independent risk factors for carotid atherosclerosis in both groups. Higher education, high salt consumption, passive smoking, family history of stroke, and transient ischemic attack were unique independent risk factors, and high-density lipoprotein cholesterol was a protective factor for carotid atherosclerosis in the urban population. This study suggests that risk factors for carotid atherosclerosis differ between urban and rural populations in North China.
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http://dx.doi.org/10.3389/fneur.2020.554778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680928PMC
November 2020

The association between maternal exposure to secondhand smoke during pregnancy and their children's cerebral palsy, Shandong, China.

Tob Induc Dis 2020 21;18:87. Epub 2020 Oct 21.

School of Medicine, Shandong University, Jinan, People's Republic of China.

Introduction: Tobacco use poses a threat to the health of pregnant women and their children. Our study assessed the association between maternal exposure to secondhand smoke (SHS) during pregnancy and children's cerebral palsy (CP) in Shandong, China.

Methods: In our observational study, 5067 mother-child pairs were included from Shandong Province, China. Mothers filled in questionnaires about exposure to SHS during pregnancy. Statistical analysis and logistic regression models were built in R program to estimate the association in adjusted odds ratio (AOR) between SHS exposure during pregnancy and risk of children's CP, after adjustment for potential confounders including delivery mode and baby's birthweight.

Results: Exposure to SHS was noted among 3663 (72.3%) of the 5067 non-smoking mothers during their pregnancy. Of the 239 CP children within the study, 192 (80.3%) were exposed to SHS during pregnancy. Children born to mothers exposed to SHS during pregnancy had a higher risk of CP (AOR=1.44; 95% CI: 1.02-2.04) than those born to non-exposed mothers, the risk increased by exposure time in the logistic regression model. The association between SHS exposure during pregnancy and CP children remained significant when adjusting for delivery mode and infant's birthweight due to their significant association with CP, with an AOR of 1.46 (95% CI: 1.13-1.91) for 1-4 days/week and 1.63 (95% CI: 1.22-2.01) for 5-7 days/week exposure to SHS.

Conclusions: Our study suggests that maternal exposure to secondhand smoke during pregnancy is associated with children's CP. Future preventive interventions of CP should include strategies that target the antenatal women who are exposed to SHS.
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http://dx.doi.org/10.18332/tid/127872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592196PMC
October 2020

An assessment of how the anterior cerebral artery anatomy impacts ACoA aneurysm formation based on CFD analysis.

Br J Neurosurg 2020 Sep 29:1-5. Epub 2020 Sep 29.

Department of Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Objective: The aim of this study was to identify independent anatomic, morphologic and hemodynamic features of the ACoA (anterior communicating artery) complex that serve as risk factors for the occurrence of ACoA aneurysms.

Methods: Fifteen consecutive patients with 15 ACoA aneurysms were included. Computational fluid dynamics (CFD) simulations based on patient-specific models were carried out using 3D time-of-flight magnetic resonance angiography (3D-TOF-MRA) images. A reverse reconstruction technique was used to generate a pre-aneurysm vessel anatomy. Geometric parameters and hemodynamic changes were compared and evaluated.

Results: The overall prevalence of symmetric, dysplastic, and absent A1 segments were 53.3%, 26.7%, and 20%. The mean wall shear stress (WSS) of the absent group (AG) was significantly higher than that of the symmetric group (SG) and dysplastic group (DG). The absolute mean A1 artery flow rate (410.2 ± 88 versus 439.4 ± 101 mL/min;  = .45) of the aneurysm side was similar between the SG and DG but significantly higher in the AG (528.1 ± 77 mL/min;  < .05). The A1-A2 angles of the aneurysm side showed no significant differences among the 3 groups ( = .32). However, the mean A1-A2 angle on the aneurysm side was smaller than the contralateral A1-A2 angle (101.9 ± 9.1˚ versus 120.3 ± 7.7˚;  <.05). A regression analysis demonstrated that high WSS was significantly associated with a large A1-A2 ratio (=0.52;  <.05).

Conclusions: ACoA aneurysms are a high-WSS pathology. Severe flow impingement and the anatomic vasculature structures play a role in triggering the occurrence of ACoA aneurysms.
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http://dx.doi.org/10.1080/02688697.2020.1821867DOI Listing
September 2020

Implementation of regional Acute Stroke Care Map increases thrombolysis rates for acute ischaemic stroke in Chinese urban area in only 3 months.

Stroke Vasc Neurol 2021 Mar 24;6(1):87-94. Epub 2020 Sep 24.

Department of Neurology, Shenyang Brain Hospital, Shenyang Medical College, Shenyang, China

Background: The rate of intravenous thrombolysis for acute ischaemic stroke remains low in China. We investigated whether the implementation of a citywide Acute Stroke Care Map (ASCaM) is associated with an improvement of acute stroke care quality in a Chinese urban area.

Methods: The ASCaM comprises 10 improvement strategies and has been implemented through a network consisting of 20 tertiary hospitals. We identified 7827 patients with ischaemic stroke admitted from April to October 2017, and 506 patients underwent thrombolysis were finally included for analysis.

Results: Compared with 'pre-ASCaM period', we observed an increased rate of administration of tissue plasminogen activator within 4.5 hours (65.4% vs 54.5%; adjusted OR, 1.724; 95% CI 1.21 to 2.45; p=0.003) during 'ASCaM period'. In multivariate analysis models, 'ASCaM period' was associated with a significant reduction in onset-to-door time (114.1±55.7 vs 135.7±58.4 min, p=0.0002) and onset-to-needle time (ONT) (169.2±58.1 vs 195.6±59.3 min, p<0.0001). Yet no change was found in door-to-needle time. Clinical outcomes such as symptomatic intracranial haemorrhage, favourable functional outcome (modified Rankin Scale ≤2) and in-hospital mortality remained unchanged.

Conclusion: The implementation of ASCaM was significantly associated with increased rates of intravenous thrombolysis and shorter ONT. The ASCaM may, in proof-of-principle, serve as a model to reduce treatment delay and increase thrombolysis rates in Chinese urban areas and possibly other highly populated Asian regions.
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http://dx.doi.org/10.1136/svn-2020-000332DOI Listing
March 2021

Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing.

Stem Cell Res 2020 10 3;48:101983. Epub 2020 Sep 3.

Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China. Electronic address:

ESRG was first identified in our previous study. It is highly expressed in human embryonic stem cells (hESCs), whereas it is significantly down-regulated in differentiated cells and is undetectable in adult tissues. To develop an hESC line for monitoring the expression of ESRG for further study of its function, we used gene editing techniques to insert fusion sequences of ESRG promoter and tdTomato fluorescent protein gene into the AAVS1 human safe harbor locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiation potential.
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http://dx.doi.org/10.1016/j.scr.2020.101983DOI Listing
October 2020

Gene Expression Profiling Studies Using Microarray in Osteoarthritis: Genes in Common and Different Conditions.

Arch Immunol Ther Exp (Warsz) 2020 Sep 10;68(5):28. Epub 2020 Sep 10.

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, 26 Heping Road, Xiangfang District, Harbin, Heilongjiang, People's Republic of China.

Osteoarthritis (OA), which is characterized mainly by cartilage degradation, is the most prevalent joint disorder worldwide. Although OA is identified as a major cause of joint pain, disability, and socioeconomic burden, the etiology of OA is still not clearly known. Recently, gene microarray analysis has become an efficient method for the research of complex diseases and has been employed to determine what genes and pathways are involved in the pathological process of OA. In this review, OA study results over the last decade are summarized for gene expression profiling of various tissues, such as cartilage, subchondral bone, and synovium in human OA and mouse OA models. Many differentially expressed genes, which mainly involve matrix metabolism, bone turnover, and inflammation pathways, were identified in diseased compared with "normal" tissues. Nevertheless, rare common genes were reported from studies using different tissue sources, microarray chips, and research designs. Thus, future novel and carefully designed microarray studies are required to elucidate underlying genetic mechanisms in the pathogenesis of OA as well as new directions for potential OA-targeted pharmaceutical therapies.
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http://dx.doi.org/10.1007/s00005-020-00592-4DOI Listing
September 2020

A phosphorescence "turn-on" probe for the detection and imaging of Al based on aggregation-induced emission.

Talanta 2020 Nov 22;219:121298. Epub 2020 Jun 22.

College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, People's Republic of China. Electronic address:

Aggregation-induced emission luminogens (AIEgens) have been widely used to design fluorescent probes for chemosensing and bioimaging. However, it is still challenging to design long-lived AIE-active probes due to the lack of aggregation-induced phosphorescence (AIP) luminogens. In this work, we design and synthesize a long-lived molecular probe with aggregation-induced phosphorescence property for aluminum ion-specific detection by introducing multiple carboxylic acid groups in a unique twisted molecular skeleton, and develop a first phosphorescent detection method for aluminum ion based on aggregation-induced emission mechanism. The introduction of six carboxylic acid groups into the probe not only significantly enhances the water-solubility but also provides specific recognition unit for aluminum ions via complexation. The probe shows a very sharp emission enhancement in the presence of aluminum ions via aluminum ion-triggered aggregation-induced emission. The cytotoxicity test of the probe shows its biocompatible nature, and further imaging results in live human cells and roots of live Arabidopsis thaliana demonstrates that the designed AIP-active probe is capable of monitoring aluminum ions in complex biological systems. This work proposes a general design strategy for AIP-active probes, and provides valuable use of these AIP-active probes in bioimaging.
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http://dx.doi.org/10.1016/j.talanta.2020.121298DOI Listing
November 2020

Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway.

ACS Chem Biol 2020 09 8;15(9):2529-2538. Epub 2020 Sep 8.

Molecular Biotechnology, Leiden University, PO Box 9505, 2300RA Leiden, The Netherlands.

Angucyclines are a structurally diverse class of actinobacterial natural products defined by their varied polycyclic ring systems, which display a wide range of biological activities. We recently discovered lugdunomycin (), a highly rearranged polyketide antibiotic derived from the angucycline backbone that is synthesized via several yet unexplained enzymatic reactions. Here, we show via , , and structural analysis that the promiscuous reductase LugOII catalyzes both a C6 and an unprecedented C1 ketoreduction. This then sets the stage for the subsequent C-ring cleavage that is key to the rearranged scaffolds of . The 1.1 Å structures of LugOII in complex with either ligand 8--Methylrabelomycin () or 8--Methyltetrangomycin () and of apoenzyme were resolved, which revealed a canonical Rossman fold and a remarkable conformational change during substrate capture and release. Mutational analysis uncovered key residues for substrate access, position, and catalysis as well as specific determinants that control its dual functionality. The insights obtained in this work hold promise for the discovery and engineering of other promiscuous reductases that may be harnessed for the generation of novel biocatalysts for chemoenzymatic applications.
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http://dx.doi.org/10.1021/acschembio.0c00564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506943PMC
September 2020

Structure basis of non-structural protein pA151R from African Swine Fever Virus.

Biochem Biophys Res Commun 2020 10 20;532(1):108-113. Epub 2020 Aug 20.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, 430062, PR China. Electronic address:

African Swine Fever Virus (ASFV) is an enveloped double-stranded DNA icosahedral virus that causes the devastating hemorrhagic fever of pigs. ASFV infections severely impact swine production and cause an enormous economic loss, but no effective vaccine and therapeutic regimen is available. pA151R is a non-structural protein of ASFV, which is expressed at both early and late stages of viral infection. Significantly, pA151R may play a key role in ASFV replication and virus assembly as suppressing pA151R expression can reduce virus replication. However, little is known about the functional and structural mechanisms of pA151R because it shares a very low sequence identity to known structures. It was proposed that pA151R might participate in the redox pathway owing to the presence of a thioredoxin active site feature, the WCTKC motif. In this study, we determined the crystal structure of pA151R. Based on the crystal structure, we found that pA151R comprises of a central five-stranded β-sheet packing against two helices on one side and an incompact C-terminal region containing the WCTKC motif on the other side. Notably, two cysteines in the WCTKC motif, an additional cysteine C116 from the β7-β8 loop together with ND1 of H109 coordinate a Zn ion to form a Zn-binding motif. These findings suggest that the structure of pA151R is significantly different from that of typical thioredoxins. Our structure should provide molecular insights into the understanding of functional and structural mechanisms of pA151R from ASFV and shall benefit the development of prophylactic and therapeutic anti-ASFV agents.
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http://dx.doi.org/10.1016/j.bbrc.2020.08.011DOI Listing
October 2020

NKL-24: A novel antimicrobial peptide derived from zebrafish NK-lysin that inhibits bacterial growth and enhances resistance against Vibrio parahaemolyticus infection in Yesso scallop, Patinopecten yessoensis.

Fish Shellfish Immunol 2020 Nov 15;106:431-440. Epub 2020 Aug 15.

Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, 116023, China. Electronic address:

The extensive use of antibiotics in aquaculture has resulted in the prevalence of antibiotic-resistant bacteria and, consequently, new antibacterial strategies or drugs with clear modes of action are urgently needed. Antimicrobial peptides (AMPs) are currently widely considered as alternatives to antibiotics in the treatment of infections in aquatic animals. In this study, we aimed to evaluate the effects of NKL-24, a truncated peptide derived from zebrafish NK-lysin, against Yesso scallop (Patinopecten yessoensis) pathogen, Vibrio parahaemolyticus. The results showed that NKL-24 had a potent antibacterial effect against V. parahaemolyticus via a membrane active cell-killing mechanism. The in vitro study showed that sub-lethal levels of NKL-24 obviously reduced bacterial swimming ability and downregulated the transcription of the selected genes associated with V. parahaemolyticus virulence. Studies on NKL-24 biosafety in hemocytes and in Yesso scallop have shown no adverse effects from this peptide. Bacteria challenge test results demonstrated that NKL-24 significantly decreased the mortality and inhibited bacterial growth in the scallop infected with V. parahaemolyticus, while further in vivo examination revealed that NKL-24 could enhance non-specific immune parameters. Moreover, NKL-24 was capable of modulating a series of V. parahaemolyticus-responsive genes in the scallop. These results suggest the protective action of NKL-24 against V. parahaemolyticus and the potential of this peptide as a promising candidate for aquaculture applications.
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http://dx.doi.org/10.1016/j.fsi.2020.08.020DOI Listing
November 2020

Elastic modulus evolution of rocks under heating-cooling cycles.

Sci Rep 2020 Aug 14;10(1):13835. Epub 2020 Aug 14.

Laboratory for Precision and Nano Processing Technologies, School of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.

Rocks decay significantly during or after heating-cooling cycles, which can in turn lead to hazards such as landslide and stone building collapse. Nevertheless, the deterioration mechanisms are unclear. This paper presents a simple and reliable method to explore the mechanical property evolutions of representative sandstones during heating-cooling cycles. It was found that rock decay takes place in both heating and cooling processes, and dramatic modulus changes occurred near the α - β phase transition temperature of quartz. Our analysis also revealed that the rock decay is mainly attributed to the internal cracking. The underlying mechanism is the heterogeneous thermal deformation of mineral grains and the α - β phase transition of quartz.
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http://dx.doi.org/10.1038/s41598-020-70920-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427969PMC
August 2020

Biocompatible and Rapid Cyclization of Peptides with 2,4-Difluoro-6-hydroxy-1,3,5-benzenetricarbonitrile for the Development of Cyclic Peptide Libraries.

Bioconjug Chem 2020 09 14;31(9):2085-2091. Epub 2020 Aug 14.

Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Xiamen University, Xiamen, 361005, P.R. China.

We report a biocompatible and rapid reaction between cysteine thiols and 2,4-difluoro-6-hydroxy-1,3,5-benzenetricarbonitrile (DFB), which enables the efficient cyclization of peptides in neutral aqueous solutions. The reaction was further applied to cyclize peptides displayed on the phage surface without reducing phage infectivity, thus affording high-quality cyclic peptide libraries useful for screening of cyclic peptide ligands. Using the DFB-cyclic peptide library, we identified ligands that can distinguish the pro-survival protein Bcl-xl from its close relative Bcl-2. Therefore, this study on one hand reports a useful reaction for the construction of cyclic peptide libraries, and on the other hand presents valuable hits for further design of selective Bcl-xl ligands.
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http://dx.doi.org/10.1021/acs.bioconjchem.0c00363DOI Listing
September 2020

An Exploratory Clinical Study on an Automated, Speed-Sensing Treadmill Prototype With Partial Body Weight Support for Hemiparetic Gait Rehabilitation in Subacute and Chronic Stroke Patients.

Front Neurol 2020 24;11:747. Epub 2020 Jul 24.

Department of Rehabilitation Medicine, Centre of Advanced Rehabilitation Therapeutics, Tan Tock Seng Hospital Rehabilitation Centre, Singapore, Singapore.

Impairments in walking speed, capacity, and endurance are commonly seen after stroke. Treadmill training improves endurance and gait speed. However, the lack of variable training speed and automated speed progression increases the risk of backward displacement and falling. An automated, speed-sensing treadmill prototype with partial body weight support, the Variable Automated Speed and Sensing Treadmill II (VASST II), was tested in an outpatient rehabilitation setting. Eleven subacute or chronic hemiplegics who could ambulate at > 0.2 m/s for >50 m participated in the study. All subjects underwent physiotherapist-supervised training on VASST II for 60 min daily, 3 times per week, for 5 weeks (total 15 h). Outcome measures at Week 3 (mid-VASST II training), Week 6 (post-VASST II training), Week 12 (first follow-up), and Week 24 (second follow-up) included the 6 minute walk test (6 MWT), 10 meter walk test (10 MWT), Berg Balance Scale (BBS) score, and Functional Ambulation category (FAC) score. User acceptability of VASST II for both study subjects and physiotherapists were also assessed. All subjects [median (IQR) age: 53.0 (22) years; median (IQR) duration post-stroke: 524 (811) days] completed VASST II training. At baseline, mean ± SD 6 MWT was 114 ± 50.9 m; mean ± SD 10 MWT was 0.37 ± 0.18 m/s; mean ± SD BBS score was 40 ± 10; and, mean ± SD FAC score was 4 ± 1. At Week 6, there were significant improvements in the 6 MWT [158.91 ± 88.69 m; = 0.003], 10 MWT [0.49 ± 0.30 m/s; = 0.016], and BBS score [42 ± 10; = 0.003]. Improvements in 6 MWT and BBS scores were sustained at Week 24, but not in the 10 MWT. No VASST II-training related falls were reported. All subjects rated their VASST II training positively and indicated that it improved their current walking ability. VASST II training was effective, feasible, and safe in patients with subacute or chronic post-stroke hemiparetic gait, with sustained gains in distance walked (6 MWT) and functional balance (BBS score) up to 19 weeks post-intervention.
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http://dx.doi.org/10.3389/fneur.2020.00747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394021PMC
July 2020

Structural insights into thebaine synthase 2 catalysis.

Biochem Biophys Res Commun 2020 08 22;529(2):156-161. Epub 2020 Jun 22.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, 430062, PR China. Electronic address:

Thebaine synthase 2 (THS2) that can transform (7S)-salutaridinol 7-O-acetate to thebaine catalyzes the final step of thebaine biosynthesis in Papaver somniferum. Here, the crystal structures of THS2 and its complex with thebaine are reported, revealing the interaction network in the substrate-binding pocket. Subsequent docking and QM/MM studies was performed to further explore the catalytic mechanism of THS2. Our results suggest that T105 may abstract the proton of C4-OH from the substrate under the assistance of H89. The resulting C4-O phenolate anion then attacks the nearby C5, and triggers intramolecular S2' syn displacement with the elimination of O-acetyl group. Moreover, the latter S2' reaction is the rate-determining step of the whole enzymatic reaction with an overall energy barrier of 18.8 kcal/mol. These findings are of pivotal importance to understand the mechanism of action of thebaine biosynthesis, and would guide enzyme engineering to enhance the production of opiate alkaloids via metabolic engineering.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.199DOI Listing
August 2020

Clinical characteristics and predictors of permanent stoma in rectal cancer patients underwent anterior resections: the value of preoperative prognostic nutritional index.

Int J Clin Oncol 2020 Nov 11;25(11):1960-1968. Epub 2020 Jul 11.

Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People's Republic of China.

Objectives: This study was aimed to investigate the correlation between preoperative prognostic nutritional index (PNI) and permanent stoma (PS) in patients with defunctioning stoma (DS) after anterior resections and, based on it, to reveal the clinical value of PNI on clinical strategies about the selection of stoma location.

Methods: A total of 281 consecutive rectal cancer patients who accepted anterior resection and DS in the National Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University from June 2008 to June 2018 were enrolled in this study. Receiver operating characteristic (ROC) curve for PNI was performed to discriminate PS. Univariate and multivariate analysis were conducted to identify the clinical characteristics and risk factors for PS. Specific reasons for patients with DS turned into PS were reviewed.

Results: ROC curve analysis defined PNI cutoff level of 45.85 corresponding to PS (area under the curve (AUC) = 0.71, 77% sensitivity, 56.9% specificity). Low PNI (OR = 3.23, P = 0.005), tumor crossing the peritoneal reflection (PR) (OR = 3.42, P = 0.003), postoperative distant metastasis (OR = 6.31, P < 0.001) were independently associated with PS. Besides, anastomotic complications (31.4%), poor oncological outcomes (35.3%), and personal preferences (33.3%) were the specific reasons for patients turning into PS.

Conclusions: Preoperative PNI is an independent prognostic factor to predict PS in patients who underwent anterior resection and DS. Therefore, combined with other clinical characteristics and predictors, preoperative measurements of PNI could provide a significant support for clinical decision on patients prepared to accept anterior resection and DS.
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http://dx.doi.org/10.1007/s10147-020-01743-5DOI Listing
November 2020

Sealed pre-carbonization to regulate the porosity and heteroatom sites of biomass derived carbons for lithium-sulfur batteries.

J Colloid Interface Sci 2020 Nov 1;579:667-679. Epub 2020 Jul 1.

School of Environment, Henan Normal University, Xinxiang, Henan 453007, PR China. Electronic address:

Ultrathin carbon sheets with high surface area, abundant mesoporous structure and rich N, O heteroatom doping sites were prepared from puffed rice through unique sealed pre-carbonization. By utilizing them as cathode materials for lithium-sulfur batteries, the in-operando Raman spectra and electrochemical tests indicated that the polysulfides shuttling effect could be substantially suppressed by the physical confinement and chemical adsorption due to these structural advantages. In addition, the well-developed hierarchical porous structure and ultrathin thickness were found to enhance the mass diffusion in the electrode, which increased the ion conductivity within the carbon framework. Therefore, the as-designed cathode was observed to accommodate the high sulfur loading amounts and demonstrated a high reversible capacity of 920.8 mAh g after 100 cycles at 0.2C with high coulombic efficiency (~100%). Moreover, the long-term stability test at 1C indicated that the cathode achieved a high reversible capacity of 702.1 mAh g after 300 cycles with low decay rate of 0.07% per cycle.
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http://dx.doi.org/10.1016/j.jcis.2020.06.068DOI Listing
November 2020