Publications by authors named "Weidong Li"

524 Publications

Boron-nitrogen-doped carbon dots on multi-walled carbon nanotubes for efficient electrocatalysis of oxygen reduction reactions.

J Colloid Interface Sci 2021 May 18;600:865-871. Epub 2021 May 18.

Green Catalysis Center, College of Chemistry, Zhengzhou University, No. 100 Science Avenue, Zhengzhou City 450001, China. Electronic address:

Cost-effective production of metal-free catalysts for the oxygen-reduction reaction (ORR), to supersede Pt-based catalysts, is challenging. Here, a three-dimensional nanocatalyst was prepared by compounding multi-wall carbon nanotubes (MWCNTs) with easily modified and doped carbon dots (CDs) as sources of B and N. The catalyst has high conductivity and a large specific surface area similar to the MWCNTs, allowing exposure of many CDs with rich edge active sites and enhancing electron transfer. The catalyst exhibits excellent ORR performance, with 0.92 V of E vs reversible hydrogen electrode (RHE). The E value exhibits a reduction of 50 mV compared with that of Pt/C (0.85 V) with a limited current density of 5.95 mA cm. The enhanced catalytic performance is attributed to the synergy of pyridine N and BC. This work describes a simple and economical strategy for metal-free catalysts, and promotes the development of such catalysts for metal-air batteries and fuel cells.
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http://dx.doi.org/10.1016/j.jcis.2021.05.089DOI Listing
May 2021

Tislelizumab Plus Chemotherapy as First-line Treatment for Locally Advanced or Metastatic Nonsquamous Non-Small Cell Lung Cancer (RATIONALE 304): A Randomized Phase 3 Trial.

J Thorac Oncol 2021 May 22. Epub 2021 May 22.

Peking Union Medical College Hospital, 41 Damucang Hutong, Xicheng District, Beijing, China 100000.

Hypothesis: Tislelizumab, an anti-PD-1 antibody, was specifically engineered to minimize FcɣR macrophages binding to abrogate antibody-dependent phagocytosis. Compared with chemotherapy alone, tislelizumab plus chemotherapy may improve clinical outcomes in patients with advanced nonsquamous non-small cell lung cancer (nsq-NSCLC).

Methods: In this open-label phase 3 trial (RATIONALE 304; NCT03663205), patients with histologically confirmed stage IIIB/IV nsq-NSCLC were randomized (2:1) to receive either Arm A: tislelizumab plus platinum (carboplatin or cisplatin) and pemetrexed every 3 weeks (Q3W) or Arm B: platinum and pemetrexed alone Q3W during induction treatment, followed by intravenous maintenance pemetrexed Q3W. The primary endpoint was progression-free response (PFS) assessed by an independent review committee; clinical response and safety/tolerability were secondary endpoints.

Results: Overall, 332 patients (n=222 [A]; n=110 [B]) received treatment. With a median study follow-up of 9.8 months, PFS was significantly longer with tislelizumab plus chemotherapy compared with chemotherapy alone (median PFS: 9.7 versus 7.6 months; HR=0.645 [95% CI: 0.462, 0.902]; P=0.0044). Additionally, response rates were higher and response duration was longer with combination therapy versus chemotherapy alone. Hematologic adverse events (AEs) were common in both treatment arms; the majority of reported AEs were grade 1-2 in severity. The most common grade ≥3 AEs were associated with chemotherapy and included neutropenia (44.6% [A]; 35.5% [B]) and leukopenia (21.6% [A]; 14.5% [B]).

Conclusions: Addition of tislelizumab to chemotherapy resulted in significantly prolonged PFS, higher response rates, and longer response duration compared with chemotherapy alone, identifying a new potential option for first-line treatment of advanced nsq-NSCLC irrespective of disease stage.
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http://dx.doi.org/10.1016/j.jtho.2021.05.005DOI Listing
May 2021

Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices.

Life Sci Alliance 2021 07 18;4(7). Epub 2021 May 18.

Department of Molecular and Cellular Physiology, Shinshu University Academic Assembly, Institute of Medicine, Shinshu University Academic Assembly, Matsumoto, Japan.

A purification protocol was developed to identify and analyze the component proteins of a postsynaptic density (PSD) lattice, a core structure of the PSD of excitatory synapses in the central nervous system. "Enriched"- and "lean"-type PSD lattices were purified by synaptic plasma membrane treatment to identify the protein components by comprehensive shotgun mass spectrometry and group them into minimum essential cytoskeleton (MEC) and non-MEC components. Tubulin was found to be a major component of the MEC, with non-microtubule tubulin widely distributed on the purified PSD lattice. The presence of tubulin in and around PSDs was verified by post-embedding immunogold labeling EM of cerebral cortex. Non-MEC proteins included various typical scaffold/adaptor PSD proteins and other class PSD proteins. Thus, this study provides a new PSD lattice model consisting of non-microtubule tubulin-based backbone and various non-MEC proteins. Our findings suggest that tubulin is a key component constructing the backbone and that the associated components are essential for the versatile functions of the PSD.
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http://dx.doi.org/10.26508/lsa.202000945DOI Listing
July 2021

[Vitamin A nutrition status and influencing factors of Chinese rural elderly in 2015].

Wei Sheng Yan Jiu 2021 Mar;50(2):186-191

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention; Key Laboratory of Trace Element Nutrition, National Health Commission of the People's Republic of China, Beijing 100050, China.

Objective: To analyze the level of vitamin A and its influencing factors of Chinese rural elderly.

Methods: The serum samples of the rural elderly were selected by random sampling method from the biological sample bank of Chinese Adults Nutrition and Chronic Diseases Surveillance in 2015(CANCDS2015). The basic information of the subjects was collected by questionnaire survey. High performance liquid chromatography(HPLC) method was used to determine the vitamin A nutritional status of the population according to the standard recommended by WHO. Multiple logistic regression analysis was explored the relationship between vitamin A status with the potential relevant factors.

Results: A total of 3134 elderly from Chinese rural were included in this study. The serum vitamin A concentration(M(P25, P75))was 1. 92(1. 50, 2. 45)μmoL/L. The prevalence of vitamin A deficiency was 0. 99%(95%CI 0. 64%-1. 34%), and the prevalence of vitamin A marginal deficiency was 4. 38%(95%CI 3. 65%-5. 10%). The prevalence of vitamin A marginal deficiency was significantly higher in elderly lived in western region than that lived in eastern and central regions of China. The serum vitamin A adequacy rate of rural elderly who were 80 years old and above or thin was relatively low(P<0. 05). Married elderly had significant lower vitamin A deficiency rate. The result from multivariate logistic regression model analysis showed that gender, age and body mass index had significant effects on vitamin A nutritional status of Chinese urban elderly(P<0. 05).

Conclusion: The nutritional status of vitamin A in rural elderly in China was better, and the deficiency rate was lower.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.004DOI Listing
March 2021

[Vitamin A nutrition status among pregnant women in rural China in 2015].

Wei Sheng Yan Jiu 2021 Mar;50(2):181-185

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention; Key Laboratory of Trace Element Nutrition, National Health Commission of the People's Republic of China, Beijing 100050, China.

Objective: To evaluate the nutrition status of vitamin A in Chinese rural pregnant women from the Chinese National Nutrition and Health Survey 2015(CNNHS 2015).

Methods: A total of 3273 Chinese rural pregnant women were recruited in this study. The concentrations of serum retinol were determined using high performance liquid chromatography. The C-reactive protein and α-acid glycoprotein were determined by immunoturbidimetric method. The distribution of serum retinol status in pregnant women was further described for different regions, age groups, nationalities, body mass index(BMI), education levels, gestational weeks, family income per capita and inflammation levels. The prevalence of vitamin A deficiency and the percentage of marginal vitamin A deficiency in pregnant women were compared in different subgroups.

Results: The median concentration of serum retinol(M(P25, P75)) was 0. 47(0. 36, 0. 61) mg/L. There were significant differences among different regions, age groups, education levels, gestational age and per capita household income(P<0. 01). The prevalence of vitamin A deficiency was 1. 2% for deficiency and 10. 5% for marginal deficiency, respectively. Significant differences were observed in age groups, gestational weeks and inflammation levels(P<0. 01). In addition, the prevalence of vitamin A deficiency was relatively serious, 2. 1% for deficiency and 14. 2% for marginal deficiency in the third trimester, 2. 0% for deficiency and 12. 2% for marginal in the 30-44 years.

Conclusion: It should be pay more attention on the vitamin A nutrition status in the third trimester and the 30-44 years old pregnant women, although the vitamin A status had been greatly improved in Chinese rural pregnant women.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.003DOI Listing
March 2021

[Zinc status of 18-60 year-old adults in China in 2015].

Wei Sheng Yan Jiu 2021 Mar;50(2):175-180

National Institute for Nutrition Health, Chinese Center for Disease Control and Prevention; Key Laboratory of Trace Element Nutrition, National Health Commission of the People's Republic of China, Beijing 100050, China.

Objective: To estimate the distribution of serum zinc levels, zinc deficiency status and possible influencing factors in 18-60 year-old adults in China.

Methods: Based on the data of China Adult Chronic Disease and Nutrition Surveillance, 3903 blood samples were selected by stratified random sampling. The distribution of serum zinc in adults with different gender, age, regional type and regional distribution were analyzed by inductively coupled plasma mass spectrometry and body mass index(BMI), exercise, smoking, drinking, vitamin A level and other factors on serum zinc concentration and zinc deficiency rate. Multivariate logistic regression model was used to analyze the correlation between zinc deficiency and the influencing factors.

Results: In 2015, the median of serum zinc in Chinese adults aged 18-60 years was 103. 44(95%CI 64. 84-186. 12) μg/dL, and the overall zinc deficiency rate was 6. 04%. Serum zinc concentration had statistically significant difference(P<0. 05) in different genders, regional types, regional distribution, vitamin A status, smoking status, drinking status. In the comparison of zinc deficiency rates, ethnic minorities(10. 67 %) was higher than Han(5. 44%), rural area(7. 35%) was higher than urban area(4. 90%), vitamin A deficiency group(9. 12%) was higher than vitamin A normal group(5. 90%), non-exercise group(6. 29%) was higher than sports group(5. 09%). Among different regional distributions, the western region had the highest zinc deficiency rate(7. 33%), and among different BMI groups overweight group had the lowest rate(4. 81%). These differences were statistically significant(P<0. 05). In the analysis of multivariate logistic regression, the risk of zinc deficiency in the vitamin A deficiency group was 1. 89 higher than that of the normal group(OR=1. 89, 95%CI 1. 13-3. 18); and the overweight group had a lower risk of serum zinc deficiency than the normal group(OR=0. 71, 95%CI 0. 52-0. 96).

Conclusion: The prevalence of serum zinc deficiency among adults aged 18-60 in China is relatively low. Vitamin A deficiency is a risk factor for zinc deficiency and overweight is a protective factor for zinc deficiency.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.002DOI Listing
March 2021

An injectable peptide hydrogel with excellent self-healing ability to continuously release salvianolic acid B for myocardial infarction.

Biomaterials 2021 Jul 29;274:120855. Epub 2021 Apr 29.

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Drug-loaded hydrogels can improve blood supply and inhibit extracellular matrix degradation after myocardial infarction. However, due to the continual dynamic motion of cardiac tissue, the hydrogel structure cannot be reconstructed in time, causing accelerated degradation and drug burst release. Here, a novel, superior, self-healing elastin-mimic peptide hydrogel (EMH) was fabricated for the local delivery of salvianolic acid B (SaB). The self-healing ability of EMH is enhanced by SaB-loaded polydopamine nanoparticles (SaB-PDA). In vitro, the pre-hydrogel (SaB-PDA/pre-EMH) is endowed with excellent biocompatibility and a low viscosity, making it suitable for intramyocardial injection. Once injected into the myocardial infarction (MI) region, SaB-PDA/pre-EMH can form SaB-PDA/EMH with great mechanical strength under the action of upregulated transglutaminase (TGase) in heart tissue post-MI. The superior self-healing ability of SaB-PDA/EMH allows for an increase in retention time in the beating ventricular wall. Therefore, with long-term release of SaB, SaB-PDA/EMH can inhibit ventricular remodeling and promote angiogenesis for MI treatment.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120855DOI Listing
July 2021

Preparation of carbon dots-hematite quantum dots-loaded hydroxypropyl cellulose-chitosan nanocomposites for drug delivery, sunlight catalytic and antimicrobial application.

J Photochem Photobiol B 2021 Jun 24;219:112201. Epub 2021 Apr 24.

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

In this project, we studied the thermal and chemical method for the synthesis of carbon dots (CDs)/Hematite (α-FeO) quantum dots and the preparation of hydroxypropyl cellulose cross-linked chitosan (HPCCS) and ulvan (UN) was performed by chemical method. Carbon dots/α-FeO quantum dots with size distribution of 3-5 nm were completely encapsulated in the HPCCS/UN NPs to obtain composites, which indicated unique characteristics with respect to antimicrobial, pH-responsive and optical properties. The CDs-HQDs/HPCCS/UN nanocomposites exhibited a single-excitation (440 nm), dual-emission fluorescence property (505 nm and 628 nm for green and red light from CDs-HQDs and HPCCS/UN NPs). The nanocomposites played as a pH-responsive drug delivery process to release ulvan at a fast rate in pH 7.4 buffer solution but at a slow rate in low pH solutions. The CDs-HQDs/HPCCS/UN nanocomposites gained the highest photocatalytic activity for degrading 4-chlorophenol (4-CPh) as a pollutant (>98% during 70 min under sunlight irradiation). Moreover, the nanocomposites indicated great inhibitory influences towards bacterial and fungal.
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http://dx.doi.org/10.1016/j.jphotobiol.2021.112201DOI Listing
June 2021

Using i-vectors from voice features to identify major depressive disorder.

J Affect Disord 2021 Jun 20;288:161-166. Epub 2021 Apr 20.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Background: Machine-learning methods using acoustic features in the diagnosis of major depressive disorder (MDD) have insufficient evidence from large-scale samples and clinical trials. This study aimed to evaluate the effectiveness of the promising i-vector method on a large sample of women with recurrent MDD diagnosed clinically, examine its robustness, and provide an explicit acoustic explanation of the i-vectors.

Methods: We collected utterances edited from clinical interview speech records of 785 depressed and 1,023 healthy individuals. Then, we extracted Mel-frequency cepstral coefficient (MFCC) features and MFCC i-vectors from their utterances. To examine the effectiveness of i-vectors, we compared the performance of binary logistic regression between MFCC i-vectors and MFCC features and tested its robustness on different utterance durations. We also determined the correlation between MFCC features and MFCC i-vectors to analyze the acoustic meaning of i-vectors.

Results: The i-vectors improved 7% and 14% of area under the curve (AUC) for MFCC features using different utterances. When the duration is > 40 s, the classification results are stabilized. The i-vectors are consistently correlated to the maximum, minimum, and deviations of MFCC features (either positively or negatively).

Limitations: This study included only women.

Conclusions: The i-vectors can improve 14% of the AUC on a large-scale clinical sample. This system is robust to utterance duration > 40 s. This study provides a foundation for exploring the clinical application of voice features in the diagnosis of MDD.
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http://dx.doi.org/10.1016/j.jad.2021.04.004DOI Listing
June 2021

sLe expression in invasive micropapillary breast carcinoma is associated with poor prognosis and can be combined with MUC1/EMA as a supplementary diagnostic indicator.

Cancer Biol Med 2021 Apr 24. Epub 2021 Apr 24.

Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer; Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education; Breast Cancer Innovation Team of the Ministry of Education; State Key Laboratory of Breast Cancer Research, Tianjin 300060, China.

Objective: Mucin 1 (MUC1/EMA) and sialyl Lewis X (sLe) indicate polarity reversal in invasive micropapillary carcinoma (IMPC). The purpose of this study was to evaluate the expression of MUC1/EMA and sLe and to assess their diagnostic and prognostic value in patients with IMPC.

Methods: The expression of sLe and MUC1/EMA in 100 patients with IMPC and a control group of 89 patients with invasive ductal carcinoma not otherwise specified (IDC-NOS) were analyzed with IHC. Fresh tumor tissues were collected from patients with IMPC or IDC-NOS for primary culture and immunofluorescence analysis.

Results: The rate of nodal metastasis was higher in patients with IMPC than those with IDC-NOS, and IMPC cells tended to express more sLe and MUC1/EMA in the cytomembranes (the stroma-facing surfaces of the micropapillary clusters) than IDC-NOS cells. In IMPC, high cytomembrane expression of sLe, but not MUC1/EMA, indicated poor prognosis. In addition, among the 100 patients with IMPC, 10 patients had sLe+/EMA- expression patterns, and 8 patients had sLe-/EMA+ expression patterns. The primary IMPC cells were suspended, non-adherent tumor cell clusters, whereas the primary IDC cells were adherent tumor cells. Immunofluorescence analysis showed that MUC1/EMA and sLex were co-expressed on the cytomembranes in IMPC cell clusters and in the cytoplasm in IDC-NOS cells.

Conclusions: sLe can be used as a prognostic indicator and can be combined with MUC1/EMA as a complementary diagnostic indicator to avoid missed IMPC diagnosis.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0422DOI Listing
April 2021

RNAi-Mediated Silencing of Pgants Shows Core 1 Glycans Are Required for Pupation in .

Front Physiol 2021 24;12:629682. Epub 2021 Mar 24.

Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Protein glycosylation is one of the most common and most important post-translational modifications. Despite the growing knowledge on glycosylation, the research on glycosylation is lagging behind. This study investigates the importance of glycosylation in the post-embryonic development of insects using the red flour beetle, , as a model. We identified 28 glycosylation-related genes (OGRGs) in the genome of the red flour beetle. 14 OGRGs were selected for functional analysis based on their involvement in the initial attachment of the carbohydrate in the different glycosylation pathways or the further elongation of the most abundant glycans and, in addition, showing severe RNAi-induced phenotypes in . The expression profile of these OGRGs was mapped throughout the developmental stages of the insect and in the different tissues of the pupa and adult. Subsequently, these genes were silenced using RNA interference (RNAi) to analyze their role in development. A broad spectrum of phenotypes was observed: from subtle effects and disrupted wing formation when silencing the genes involved in mannosylation, to blockage of pupation and high mortality after silencing of the genes involved in -GalNAc and core 1 -glycan (-GalNAc-Gal) synthesis. RNAi experiments were also performed to assess the effects of blocking multiple pathways of -glycosylation. However, the observed phenotypes induced by multiple RNAi were similar to those of the single gene RNAi experiments. The silencing of OGRGs often resulted in high mortality and wing phenotypes, indicating the importance of -glycosylation for the survival of the insect and the formation of wings during the post-embryonic development of .
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http://dx.doi.org/10.3389/fphys.2021.629682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024498PMC
March 2021

The reference genome and transcriptome of the limestone langur, Trachypithecus leucocephalus, reveal expansion of genes related to alkali tolerance.

BMC Biol 2021 Apr 8;19(1):67. Epub 2021 Apr 8.

Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi, Nanning, Guangxi, 530003, China.

Background: Trachypithecus leucocephalus, the white-headed langur, is a critically endangered primate that is endemic to the karst mountains in the southern Guangxi province of China. Studying the genomic and transcriptomic mechanisms underlying its local adaptation could help explain its persistence within a highly specialized ecological niche.

Results: In this study, we used PacBio sequencing and optical assembly and Hi-C analysis to create a high-quality de novo assembly of the T. leucocephalus genome. Annotation and functional enrichment revealed many genes involved in metabolism, transport, and homeostasis, and almost all of the positively selected genes were related to mineral ion binding. The transcriptomes of 12 tissues from three T. leucocephalus individuals showed that the great majority of genes involved in mineral absorption and calcium signaling were expressed, and their gene families were significantly expanded. For example, FTH1 primarily functions in iron storage and had 20 expanded copies.

Conclusions: These results increase our understanding of the evolution of alkali tolerance and other traits necessary for the persistence of T. leucocephalus within an ecologically unique limestone karst environment.
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http://dx.doi.org/10.1186/s12915-021-00998-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034193PMC
April 2021

Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.

Int J Biol Macromol 2020 Dec 25;165(Pt B):2621-2629. Epub 2020 Oct 25.

School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. Electronic address:

Many effects of Coprinus comatus are attributed to its polysaccharide components. Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs). The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated. At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared. The structural analysis exhibited that there were slight differences in the two prepared polysaccharides. However, both ICPs and CBPs could be utilized by these two strains. Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05). Therefore, this work demonstrates that ICPs could be an equivalent replacer for CBPs.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.163DOI Listing
December 2020

Elevated HB-EGF expression in neural stem cells causes middle age obesity by suppressing Hypocretin/Orexin expression.

FASEB J 2021 Apr;35(4):e21345

Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China.

Obesity is common in the middle aged population and it increases the risks of diabetes, cardiovascular diseases, certain cancers, and dementia. Yet, its etiology remains incompletely understood. Here, we show that ectopic expression of HB-EGF, an important regulator of neurogenesis, in Nestin neuroepithelial progenitors with the Cre-LoxP system leads to development of spontaneous middle age obesity in male mice accompanied by hyperglycemia and insulin resistance. The Nestin-HB-EGF mice show decreases in food uptake, energy expenditure, and physical activity, suggesting that reduced energy expenditure underlies the pathogenesis of this obesity model. However, HB-EGF expression in appetite-controlling POMC or AgRP neurons or adipocytes fails to induce obesity. Mechanistically, HB-EGF suppresses expression of Hypocretin/Orexin, an orexigenic neuropeptide hormone, in the hypothalamus of middle aged Nestin-HB-EGF mice. Hypothalamus Orexin administration alleviates the obese and hyperglycemic phenotypes in Nestin-HB-EGF mice. This study uncovers an important role for HB-EGF in regulating Orexin expression and energy expenditure and establishes a midlife obesity model whose pathogenesis involves age-dependent changes in hypothalamus neurons.
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http://dx.doi.org/10.1096/fj.202001945RDOI Listing
April 2021

Metrological Detection of Multipartite Entanglement from Young Diagrams.

Phys Rev Lett 2021 Feb;126(8):080502

Laboratoire Kastler Brossel, ENS-Université PSL, CNRS, Sorbonne Université, Collège de France, 24 Rue Lhomond, 75005 Paris, France.

We characterize metrologically useful multipartite entanglement by representing partitions with Young diagrams. We derive entanglement witnesses that are sensitive to the shape of Young diagrams and show that Dyson's rank acts as a resource for quantum metrology. Common quantifiers, such as the entanglement depth and k-separability are contained in this approach as the diagram's width and height. Our methods are experimentally accessible in a wide range of atomic systems, as we illustrate by analyzing published data on the quantum Fisher information and spin-squeezing coefficients.
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http://dx.doi.org/10.1103/PhysRevLett.126.080502DOI Listing
February 2021

Pharmacological rescue in patient iPSC and mouse models with a rare DISC1 mutation.

Nat Commun 2021 03 3;12(1):1398. Epub 2021 Mar 3.

Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School for Medicine, University of Pennsylvania, Philadelphia, PA, USA.

We previously identified a causal link between a rare patient mutation in DISC1 (disrupted-in-schizophrenia 1) and synaptic deficits in cortical neurons differentiated from isogenic patient-derived induced pluripotent stem cells (iPSCs). Here we find that transcripts related to phosphodiesterase 4 (PDE4) signaling are significantly elevated in human cortical neurons differentiated from iPSCs with the DISC1 mutation and that inhibition of PDE4 or activation of the cAMP signaling pathway functionally rescues synaptic deficits. We further generated a knock-in mouse line harboring the same patient mutation in the Disc1 gene. Heterozygous Disc1 mutant mice exhibit elevated levels of PDE4s and synaptic abnormalities in the brain, and social and cognitive behavioral deficits. Pharmacological inhibition of the PDE4 signaling pathway rescues these synaptic, social and cognitive behavioral abnormalities. Our study shows that patient-derived isogenic iPSC and humanized mouse disease models are integral and complementary for translational studies with a better understanding of underlying molecular mechanisms.
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http://dx.doi.org/10.1038/s41467-021-21713-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930023PMC
March 2021

Depression-/Anxiety-Like Behavior Alterations in Adult Slit2 Transgenic Mice.

Front Behav Neurosci 2020 5;14:622257. Epub 2021 Feb 5.

School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou, China.

Slit2 is a member of the Slit family of secreted glycoproteins that plays highly conserved roles in neuronal axon guidance and cellular migration. Our previous experimental results showed Alzheimer's disease-like alterations and increased permeability of the blood-brain barrier in Slit2-overexpressing transgenic (Slit2-Tg) mice aged 8-9 months. Nevertheless, relatively little is known about behavioral alterations in adult Slit2-Tg mice (2-6 months of age). To observe the age-related behavioral effects of Slit2 overexpression in adult mice, we performed a battery of behavioral tests with adult Slit2-Tg mice at 2-6 months of age. The body weight of Slit2-Tg mice was lower than that of the wild-type mice from 15 weeks of age. Compared with the control mice, depression-like behaviors were found in Slit2-Tg mice from 15 to 21 weeks of age in the sucrose preference test, although Slit2-Tg mice were hyperactive in the tail suspension test. The anxiety-like behaviors were found in Slit2-Tg mice in the open field test, as well as increased locomotor activity. The anxiety-like behaviors were also found in adult Slit2-Tg mice in the elevated plus maze. Compared to wild-type mice at 23 weeks old, impairment of the hippocampal neurons were found in Slit2-Tg mice at the same age in hematoxylin-eosin staining (H&E), including some eccentric dispersion and expansion of neuronal bodies. In addition, the messenger RNA (mRNA) expression of TNF-α was elevated in the hippocampus of adult Slit2-Tg mice. Slit2 overexpression causes depression-/anxiety-like behaviors in adult mice that may be related to an increase in inflammatory factors and damage to hippocampal neurons.
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http://dx.doi.org/10.3389/fnbeh.2020.622257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892588PMC
February 2021

Circadian clock gene BMAL1 controls testosterone production by regulating steroidogenesis-related gene transcription in goat Leydig cells.

J Cell Physiol 2021 Feb 17. Epub 2021 Feb 17.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.

Testosterone is produced by Leydig cells (LCs) and undergoes diurnal changes in serum levels in rats, mice, and humans, but little is known in goats. The present study revealed that goat serum testosterone levels displayed diurnal rhythmic changes (peak time at ZT11.2). Immunohistochemical staining showed that BMAL1, a circadian clock protein, is highly expressed in goat LCs. ELISA revealed that both hCG (0-5 IU/ml) and 22R-OH-cholesterol (0-30 μM) addition stimulated testosterone synthesis in primary goat LCs in a dose-dependent manner. Treating goat LCs with hCG (5 IU/ml) significantly increased intracellular cAMP levels. Additionally, real-time quantitative polymerase chain reaction (PCR) analysis revealed that the circadian clock (BMAL1, PER1, PER2, DBP, and NR1D1) and steroidogenesis-related genes (SF1, NUR77, StAR, HSD3B2, CYP17A1, CYP11A1, and HSD17B3) showed rhythmic expression patterns in goat LCs following dexamethasone synchronization. Several Bmal1-Luc circadian oscillations were clearly observed in dexamethasone-treated goat LCs transfected with the pLV6-Bmal1-Luc plasmid. BMAL1 knockdown significantly downregulated mRNA levels of PER2, NR1D1, DBP, StAR, HSD3B2, SF1, NUR77, and GATA4, and dramatically decreased StAR and HSD3B2 protein levels and testosterone production. In contrast, BMAL1 overexpression significantly increased the mRNA and protein expression levels of StAR and HSD17B3 and enhanced testosterone production. Reporter assays revealed that goat BMAL1, or in combination with mouse CLOCK, activated goat HSD17B3 transcription in vitro. These data indicate that BMAL1 contributes to testosterone production by regulating transcription of steroidogenesis-related genes in goat LCs, providing a basis for further exploring the underlying mechanism by which the circadian clock regulates ruminant reproductive capability.
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http://dx.doi.org/10.1002/jcp.30334DOI Listing
February 2021

Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B).

Stem Cell Res 2021 Apr 2;52:102222. Epub 2021 Feb 2.

Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland; FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland. Electronic address:

NRXN1 encodes thousands of splicing variants categorized into long NRXN1α, short NRXN1β and extremely short NRXN1γ, which exert differential roles in neuronal excitation/inhibition. NRXN1α deletions are common in autism spectrum disorder (ASD) and other neurodevelopmental/neuropsychiatric disorders. We derived induced pluripotent stem cells (iPSCs) from one sibling control and two ASD probands carrying NRXN1α, using non-integrating Sendai viral method. All iPSCs highly expressed pluripotency markers and could be differentiated into ectodermal/mesodermal/endodermal cells. The genotype and karyotype of the iPSCs were validated by whole genome SNP array. The availability of the iPSCs offers an opportunity for understanding NRXN1α function in human neurons and in ASD.
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http://dx.doi.org/10.1016/j.scr.2021.102222DOI Listing
April 2021

Comparison of Effects of Sodium Chloride and Potassium Chloride on Spray Drying and Redispersion of Cellulose Nanofibrils Suspension.

Nanomaterials (Basel) 2021 Feb 9;11(2). Epub 2021 Feb 9.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

Cellulose nanofibrils (CNFs) were exposed to the same levels of potassium chloride (KCl) and sodium chloride (NaCl) before being subjected to spray drying. The effect of NaCl and KCl on the size of atomized droplets and the hydrogen bond retardation between CNFs was investigated by characterizing product morphology, particle size distribution, dispersion stability in aqueous system, and surface chemistry. The results showed that the CNF suspensions treated with KCl could be atomized into smaller droplets during spray drying, and then CNF powder with smaller sizes could be obtained. As the agglomeration was less, and the CNF with KCl addition had good dispersion stability after redispersion compared with CNF treated by NaCl. Therefore, KCl treatment was an effective method to reduce the agglomeration of CNF during spray drying.
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http://dx.doi.org/10.3390/nano11020439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916111PMC
February 2021

Comparison of clinical and serological features of RT-PCR positive and negative COVID-19 patients.

J Int Med Res 2021 Feb;49(2):300060520972658

Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China.

Background: In December 2019, an outbreak of coronavirus disease 2019 (COVID-19) began in Wuhan, China, and led to a global epidemic. We aimed to compare the clinical and serological features of COVID-19 patients with positive and negative reverse transcriptase polymerase chain reaction (RT-PCR) tests.

Methods: This was a retrospective cohort study conducted from 9 February to 4 April 2020. COVID-19 patients at Leishenshan Hospital in Wuhan, China (125 total cases; 87 RT-PCR positive and 38 RT-PCR negative) were included. COVID-19 serology was assessed by colloidal gold assay. All cases were analyzed for demographic, clinical, and serological features.

Results: There were no significant differences in most demographic features, clinical symptoms, complications or treatments of RT-PCR positive and negative COVID-19 patients. Serum IgM/IgG was positive in 82 (94%) and 33 (87%) RT-PCR positive and negative cases, respectively. IgM was detectable as early as 3 days after symptom onset and was undetectable 60 days after symptom onset. By contrast, IgG could be detected only 10 days after symptom onset and reached its peak 60 days after symptom onset.

Conclusions: Serological tests performed during the appropriate time window of disease progression could be valuable auxiliary methods to RT-PCR in COVID-19 patients.
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http://dx.doi.org/10.1177/0300060520972658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871088PMC
February 2021

Identification of the raw and processed Crataegi Fructus based on the electronic nose coupled with chemometric methods.

Sci Rep 2021 Jan 20;11(1):1849. Epub 2021 Jan 20.

State Key Lab of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, China.

Crataegi Fructus (CF) is widely used as a medicinal and edible material around the world. Currently, different types of processed CF products are commonly found in the market. Quality evaluation of them mainly relies on chemical content determination, which is time and money consuming. To rapidly and nondestructively discriminate different types of processed CF products, an electronic nose coupled with chemometrics was developed. The odour detection method of CF was first established by single-factor investigation. Then, the sensor array was optimised by a stepwise discriminant analysis (SDA) and analysis of variance (ANOVA). Based on the best-optimised sensor array, the digital and mode standard were established, realizing the odour quality control of samples. Meanwhile, mathematical prediction models including the discriminant formula and back-propagation neural network (BPNN) model exhibited good evaluation with a high accuracy rate. These results suggest that the developed electronic nose system could be an alternative way for evaluating the odour of different types of processed CF products.
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http://dx.doi.org/10.1038/s41598-020-79717-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817683PMC
January 2021

Associations between first-trimester intrauterine hematoma and twin pregnancy outcomes: a retrospective cohort study.

BMC Pregnancy Childbirth 2021 Jan 11;21(1):46. Epub 2021 Jan 11.

Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, Guangdong Province, China.

Background: In recent years, first-trimester intrauterine hematoma (IUH) has become increasingly common in twin pregnancy. The majority of studies on IUH have excluded twin pregnancies, and others did not differentiate between singleton and twin pregnancies. The impact of IUH on twin pregnancy is unclear. Therefore, the primary objective of our study was to examine associations between first-trimester IUH and pregnancy outcomes in twin pregnancies.

Methods: The data of 1020 twin pregnancies in women who received a routine examination from January 2014 to December 2018 were reviewed. We compared baseline data and pregnancy outcomes between those with and without IUH. Multivariable logistic regression analysis was used to adjust for possible confounding factors.

Results: A total of 209 patients (21.3%) developed IUH in the first trimester. First-trimester IUH was significantly associated with increased odds of miscarriage (adjusted odds ratio 14.27, 95% CI 8.25-24.70) and vanishing twin syndrome (adjusted odds ratio 3.26, 95% CI 1.11-4.61). However, there were no differences in the rates of stillbirth, preeclampsia, preterm labor (< 34 weeks), low birth weight, postpartum hemorrhage or fetal distress between the two groups. Maternal age, previous preterm birth, chorionicity in twins and the gestational week at first ultrasound did not differ between the two groups. The women with IUH had high rates of previous miscarriage (46.73% vs 38.37%, p = 0.01), assisted conception (48.56% vs 32.60%, p < 0.001) and accompanied vaginal bleeding (67.46% vs 13.43%, p < 0.001). According to the logistic regression analyses, these characteristics were not associated with pregnancy loss or vanishing twin syndrome. No IUH characteristics, including volume, largest diameter, or the presence of vaginal bleeding, were associated with pregnancy loss or vanishing twin syndrome before 20 weeks of gestation (P > 0.05).

Conclusion: In women with twin pregnancy, the presence of IUH in the first trimester was associated with the loss of one or both fetuses before 20 weeks of gestation. However, previous miscarriage, the conception method, the IUH size and the presence of vaginal bleeding were not independently associated with miscarriage or vanishing twin syndrome.
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http://dx.doi.org/10.1186/s12884-020-03528-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802326PMC
January 2021

[Nutritional analysis of eight kinds of prefabricated beef steaks].

Wei Sheng Yan Jiu 2020 Nov;49(6):969-977

Center for Disease Control and Prevention of Anhui Province, Hefei 230601, China.

Objective: A investigate on the nutritional quality of the prefabricated beef steak in the market was conducted, aiming to provide scientific basis for consumers in the consumption of prefabricated beef steaks.

Methods: The basic nutriments, amino acids, fatty acids, vitamins and mineral elements of 8 kinds of prefabricated beef steaks were analyzed and evaluated.

Results: It was showed that the contents of crude ash(1. 3-2. 4 g/100 g edible) and crude fat(1. 3-10. 0 g/100 g edible) in the 8 kinds of prefabricated beef steaks were higher than those of raw beef, while the contents of protein(11. 4-17. 2 g/100 g edible) was lower than those of raw beef. All the 8 kinds of prefabricated beef steaks had 18 kinds of amino acids and the content of histidine in children's steak(0. 57 g/100 g edible) was the highest. With the exception of snowflake steak and bulk steak, the first limiting amino acid of other 6 kinds of prefabricated beef steaks was valine. The essential amino acid composition of the 8 kinds of prefabricated steaks was close to that of FAO/WHO standard protein. This meant they were all high quality protein sources. The proportion of saturated fatty acids(48. 0%-62. 0%) was the highest, followed by monounsaturated fatty acids(32. 2%-46. 6%) and polyunsaturated fatty acids(0. 7%-7. 6%). All the 8 kinds of prepared beef steak didn't meet the S∶M∶P standard recommended by American Heart Association. There were differences in the content of vitamins among 8 kinds of prefabricated beef steaks. In terms of mineral elements, the content of Na(344-689 mg/100 g edible), Ca(6-15 mg/100 g edible), Fe(1. 0-1. 9 mg/100 g edible), Cu(0. 029-0. 050 mg/100 g edible), Mn(0. 056-0. 183 mg/100 g edible) in prefabricated steak was higher than that in raw beef.

Conclusion: There were interspecific differences in the nutritional element content and quality among different kinds of prefabricated steaks. Compared with the raw beef, the fat content was higher and protein content was lower, the composition of fatty acids was less reasonable than fish and pork.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.06.017DOI Listing
November 2020

[Nutritional components of five main products of freshwater shrimp in Anhui Province].

Wei Sheng Yan Jiu 2020 Nov;49(6):962-968

Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.

Objective: To analyze and evaluate the nutritional composition of five main products of freshwater shrimp including crawfish, river prawn, macrobrachium, freshwater shrimp, exopalaemon modestus in Anhui Province.

Methods: Conventional and biochemical analytical method were used to detect the moisture, ash, crude protein, crude fat, amino acid, fatty acid, minerals and vitamin.

Results: The contents of crude protein, crude fat, ash of the five freshwater shrimp were 13. 9-20. 8 g/100 g edible, 0. 6-2. 2 g/100 g edible, 1. 5-4. 1 g/100 g edible, respectively. A total of 18 amino acids were detected, the content of which were 11600-18600 mg/100 g edible. The amount of nine essential amino acid was measured to be 5336-9342 mg/100 g edible. The main restrictive amino acids in the five kinds of shrimps were methionine and cysteine, threonine and valine. The essential amino acid indexes in five kinds of shrimps(EAAI) were 39. 62 and 31. 81, 28. 65, 44. 85, and 33. 48, respectively. Fourteen types of fatty acid were detected. The total content of fatty acid was 287-1851 mg/100 g edible. The monounsaturated fatty acid(MUFA) and the polyunsaturated fatty acid(PUFA) were 12-234 mg/100 g edible, 164-377 mg/100 g edible, respectively. Nine minerals were detected which including five microelements and four microelements. The content of calcium in river prawn, macrobrachium and exopalaemon modestus was higher than that in crawfish and freshwater shrimp. The content of phosphorus, kalium, sodium, magnesium in five freshwater shrimp were 221-296 mg/100 g edible, 196-278 mg/100 g edible, 120. 7-202. 7 mg/100 g edible, 37-95 mg/100 g edible, respectively. The content of iron, manganese, cuprum in crawfish were higher than the others. The content of zinc in river prawn and exopalaemon modestus was high. Moreover, the vitamin B_2 and E were also detected in five freshwater shrimp. The content of vitamin B_2 and E were 0. 02-0. 07 mg/100 g edible and 1. 61-3. 30 mg/100 g edible.

Conclusion: Five main products of freshwater shrimp in Anhui belong to super nutritional value food which contained high protein, variety of amino acid, low fat, a certain amount of vitamin B_2 and E, rich in minerals.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.06.016DOI Listing
November 2020

Surgical treatment for anomalous origin of the right coronary artery from the pulmonary artery: a case report with five-year follow-up.

J Cardiothorac Surg 2021 Jan 6;16(1). Epub 2021 Jan 6.

Department of Cardiovascular Surgery, the First Affiliated Hospital, College of Medicine, Zhejiang University, Postal Address: 79#, Qingchun Road, Hangzhou, 310000, Zhejiang, China.

Background: Anomalous origin of the right coronary artery from the pulmonary artery (ARCAPA) is a rare congenital heart disease affecting about 0.002% of the population. Knowledge of ARCAPA is almost collected from case reports. The aim of this study was to provide a rare case to better understand this rare congenital coronary anomaly.

Case Presentation: We report a rare case of an 18-year-old male who was initially referred because of heart murmur. Dilated and tortuous coronary arteries were detected by echocardiography and congenital coronary anomaly was suggested. Further coronary CT angiography confirmed the diagnosis of ARCAPA. Although dual coronary system provides favorable long-term outcome, bypass surgery was considered technically difficult due to the huge mismatch of caliber between the right coronary artery and graft vessels. Eventually, simple right coronary artery ligation was performed. The patient was followed up for about 5 years without evidence of atherosclerosis or myocardial ischemia.

Conclusions: ARCAPA presents as a rare congenital heart disease with variable clinical manifestations. Surgical treatment is highly recommended to re-establish dual coronary system and prevent further complications. To our best knowledge, only about 200 cases of ARCAPA has been reported.
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http://dx.doi.org/10.1186/s13019-020-01374-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789775PMC
January 2021

Epidemiological profile of Plasmodium ovale spp. imported from Africa to Anhui Province, China, 2012-2019.

Malar J 2021 Jan 6;20(1):15. Epub 2021 Jan 6.

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, China.

Background: Although autochthonous malaria cases are no longer reported in Anhui Province, China, imported malaria has become a major health concern. The proportion of reported malaria cases caused by Plasmodium ovale spp. increased to levels higher than expected during 2012 to 2019, and showed two peaks, 19.69% in 2015 and 19.35% in 2018.

Methods: A case-based retrospective study was performed using data collected from the China Information System for Disease Control and Prevention (CISDCP) and Information System for Parasitic Disease Control and Prevention (ISPDCP) from 2012 to 2019 to assess the trends and differences between Plasmodium ovale curtisi (P. o. curtisi) and Plasmodium ovale wallikeri (P. o. wallikeri). Epidemiological characteristics were analyzed using descriptive statistics.

Results: Plasmodium o. curtisi and P. o. wallikeri were found to simultaneously circulate in 14 African countries. Among 128 patients infected with P. ovale spp., the proportion of co-infection cases was 10.16%. Six cases of co-infection with P. ovale spp. and P. falciparum were noted, each presenting with two clinical attacks (the first attack was due to P. falciparum and the second was due to P. ovale spp.) at different intervals. Accurate identification of the infecting species was achieved among only 20.00% of cases of P. ovale spp. infection. At the reporting units, 32.17% and 6.96% of cases of P. ovale spp. infection were misdiagnosed as P. vivax and P. falciparum infections, respectively.

Conclusion: The present results indicate that the potential of P. ovale spp. to co-infect with other Plasmodium species has been previously underestimated, as is the incidence of P. ovale spp. in countries where malaria is endemic. P. o. curtisi may have a long latency period of > 3 years and potentially cause residual foci, thus posing challenges to the elimination of malaria in P. ovale spp.-endemic areas. Considering the low rate of species identification, more sensitive point-of-care detection methods need to be developed for P. ovale spp. and introduced in non-endemic areas.
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http://dx.doi.org/10.1186/s12936-020-03551-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788861PMC
January 2021

LncRNA CRNDE attenuates chemoresistance in gastric cancer via SRSF6-regulated alternative splicing of PICALM.

Mol Cancer 2021 01 4;20(1). Epub 2021 Jan 4.

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

De novo and acquired resistance, which are mainly mediated by genetic alterations, are barriers to effective routine chemotherapy. However, the mechanisms underlying gastric cancer (GC) resistance to chemotherapy are still unclear. We showed that the long noncoding RNA CRNDE was related to the chemosensitivity of GC in clinical samples and a PDX model. CRNDE was decreased and inhibited autophagy flux in chemoresistant GC cells. CRNDE directly bound to splicing protein SRSF6 to reduce its protein stability and thus regulate alternative splicing (AS) events. We determined that SRSF6 regulated the PICALM exon 14 skip splice variant and triggered a significant S-to-L isoform switch, which contributed to the expression of the long isoform of PICALM (encoding PICALML). Collectively, our findings reveal the key role of CRNDE in autophagy regulation, highlighting the significance of CRNDE as a potential prognostic marker and therapeutic target against chemoresistance in GC.
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http://dx.doi.org/10.1186/s12943-020-01299-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780690PMC
January 2021

Long intergenic non-protein coding RNA 960 regulates cancer cell viability, migration and invasion through modulating miR-146a-5p/interleukin 1 receptor associated kinase 1 axis in pancreatic ductal adenocarcinoma.

Bioengineered 2021 Dec;12(1):369-381

Department of Gastroenterology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology , Guangzhou, China.

Long non-coding RNAs (lncRNAs) are considered as crucial regulatory factors in cancer biology. However, the biological function of long intergenic non-protein coding RNA 960 (LINC00960) in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC) is still unknown. The goal of this study is to investigate the role of LINC00960 in PDAC. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine the expression levels of LINC00960 in PDAC tissues and cell lines. After transfection, the loss-of-function models of LINC00960 or interleukin 1 receptor-associated kinase 1 (IRAK1) were established with BxPC-3 cells and Colo357 cells, and the malignant phenotypes of BxPC-3 cells and Colo357 cells were detected by CCK-8 assay, BrdU assay and Transwell assay, respectively. The interactions among LINC00960, miR-146a-5p and IRAK1 were predicted by bioinformatics analysis, and verified by luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. The regulatory functions of LINC00960 and miR-146a-5p on IRAK1 were detected by Western blot. We demonstrated that the LINC00960 expression was increased in PDAC tissues and cell lines. Knocking down LINC00960 or IRAK1 could repress the viability, migration, and invasion of BxPC-3 and Colo357 cells. LINC00960 functioned as a molecular sponge for miR-146a-5p, and IRAK1 was verified as a target gene of miR-146a-5p. Additionally, LINC00960 could up-regulate IRAK1 expression via repressing miR-146a-5p, and the oncogenic properties of LINC00960 were partly reversed by miR-146a-5p. Our findings reveal that LINC00960 is a promoter of PDAC progression through regulating miR-146a-5p/IRAK1axis.
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http://dx.doi.org/10.1080/21655979.2020.1868742DOI Listing
December 2021