Publications by authors named "Weidong Chen"

341 Publications

microRNA-6785-5p-loaded human umbilical cord mesenchymal stem cells-derived exosomes suppress angiogenesis and metastasis in gastric cancer via INHBA.

Life Sci 2021 Feb 17:119222. Epub 2021 Feb 17.

Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.

Objective: Exosomes (Exos) are known to transfer microRNAs (miRNAs) to participate in human diseases. We aim to identify the role of human umbilical cord mesenchymal stem cells (HUCMSCs)-derived Exos (HUCMSC-Exos) conveying miR-6785-5p in gastric cancer (GC).

Methods: MiR-6785-5p and inhibin subunit beta A (INHBA) expression in GC tissues and cells were determined. GC cells were transfected with the vectors that altered miR-6785-5p or INHBA expression. HUCMSCs were transfected with altered miR-6785-5p or INHBA vectors, and the HUCMSC-Exos were extracted. Then, HUCMSC-Exos were co-cultured with GC cells. The proliferation, migration, invasion, apoptosis and angiogenesis of GC cells were assessed. The binding relationship between miR-6785-5p and INHBA was verified.

Results: MiR-6785-5p was down-regulated and INHBA was up-regulated in GC tissues and cells. Elevation of miR-6785-5p or inhibition of INHBA restricted the malignant development of GC cells. HUCMSC-Exos suppressed malignant episodes of GC cells, which could be further enhanced by up-regulated miR-6785-5p or down-regulated INHBA. Elevated INHBA abolished the impacts of up-regulated miR-6785-5p in HUCMSC-Exos on GC cells. INHBA was confirmed as a target gene of miR-6785-5p.

Conclusion: HUCMSC-Exos containing elevated miR-6785-5p suppress angiogenesis and metastasis in GC via inhibiting INHBA. This study may further the understanding on molecular mechanisms of GC.
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http://dx.doi.org/10.1016/j.lfs.2021.119222DOI Listing
February 2021

Synthesis of Paeonol-Ozagrel Conjugate: Structure Characterization and Anti-Ischemic Stroke potential.

Front Pharmacol 2020 1;11:608221. Epub 2021 Feb 1.

College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China.

Ischemic stroke is a common neurological disease that can lead to mortality and disability. The current curative effect remains unsatisfactory because drug accumulation in the diseased areas is insufficient as a result of the unique blood-brain barrier. Therefore, much attention has been paid to develop a novel therapeutic compound, paeonol-ozagrel conjugate (POC), for ischemic stroke. Then, POC was successfully synthesized by conjugating of paeonol and ozagrel as mutual prodrug. A series of characterizations and evaluations, including high - resolution mass spectroscopy, nuclear magnetic resonance spectroscopy, partition coefficient, and assessment of cytotoxicity against PC12 cells, were performed. Pharmacokinetic study demonstrated POC is eliminated quickly (t = 53.46 ± 19.64 min), which supported a short dosing interval. The neurological score, infarct volume, histopathological changes, oxidative stress, inflammatory cytokines levels, and TXA levels also were evaluated in middle cerebral artery occlusion (MCAO) rats. All results showed that POC had a significant curative and therapeutic effect on ischemic stroke, as evaluated by the middle cerebral artery occlusion. Overall, POC can be expected to become a new drug candidate for the treatment of ischemic stroke.
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http://dx.doi.org/10.3389/fphar.2020.608221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883289PMC
February 2021

Oral Delivery of Gambogenic Acid by Functional Polydopamine Nanoparticles for Targeted Tumor Therapy.

Mol Pharm 2021 Mar 14;18(3):1470-1479. Epub 2021 Feb 14.

School of Pharmacy, Anhui University of Chinese Medicine, Anhui Academy of Chinese Medicine, Hefei, Anhui 230012, China.

To enhance the water solubility, oral bioavailability, and tumor targeting of gambogenic acid (GNA), polydopamine nanoparticles (PDA NPs) were prepared to encapsulate and stabilize GNA surface modified by folic acid (FA) and then coated with sodium alginate (GNA@PDA-FA SA NPs) to achieve an antitumor effect by oral administration. GNA@PDA-FA SA NPs exhibited pH-sensitive release behavior. cell studies manifested that GNA@PDA-FA NPs had higher cytotoxicity to 4T1 cells compared with raw GNA (IC = 2.58 μM 7.57 μM). After being modified with FA, GNA@PDA-FA NPs were taken up easily by 4T1 cells. studies demonstrated that the area under the curve (AUC) of the plasma drug concentration-time of GNA@PDA-FA SA NPs was 2.97-fold higher than that of raw GNA, along with improving drug distribution in the liver, lung, and kidney tissues. anti-tumor experiments, GNA@PDA-FA SA NPs significantly inhibited the growth of breast tumors in the 4T1 xenograft breast cancer model oral administration without obvious toxicity on major organs. Our studies indicated that the GNA@PDA-FA SA NPs modified with FA and coated with SA were a promising drug delivery system for targeting tumor therapy oral administration.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00030DOI Listing
March 2021

Regulative effect of Taohong Siwu decoction on extracellular matrix of endometrium in drug-induced abortion.

J Tradit Chin Med 2021 02;41(1):98-106

Anhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.

Objective: To explore the effects of Taohong Siwu decoction (, THSWD) on the extracellular matrix of endometrium in rats following drug-induced abortion.

Methods: Thirty-six pregnant female rats were administered mifepristone and misoprostol to induce abortion, and amounts of uterine bleeding were recorded. Pathological damage and collagen accumulation were detected by hematoxylin-eosin staining and Masson's trichrome staining in uterus, respectively. Myeloperoxidase was evaluated by immunohistochemistry.The expression levels of fibronectin, laminin, matrix metalloproteinase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1) were quantified using western blotting.

Results: THSWD could promote endometrial protection in rats following drug-induced abortion. The contents of cellulose and myeloperoxidase were significantly decreased in uterine tissue of THSWD-treated groups. Moreover, THSWD significantly decreased the expression levels of fibronectin, laminin, and TIMP-1. THSWD also significantly increased MMP-9 expression and the MMP-9/TIMP1 ratio.

Conclusion: THSWD plays a critical role in endometrial protection by reducing extracellular matrix deposition and uterine fibrosis. These effects may have been achieved by increasing MMP-9, reducing TIMP-1, and/or altering the ratio of MMP-9/TIMP-1.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2021.01.012DOI Listing
February 2021

The prognostic value of circular RNA regulatory genes in competitive endogenous RNA network in gastric cancer.

Cancer Gene Ther 2021 Jan 29. Epub 2021 Jan 29.

Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, China.

Accumulating evidence shows that circular RNA (circRNA) is an important regulator of many diseases, especially cancer. Gastric cancer (GC) is a malignant tumor of the digestive system. The regulatory role and potential mechanism of circRNAs in GC remain unknown. This study aims to explore the function and regulatory mechanism of circRNA-related competitive endogenous RNA (ceRNA) in GC. The circRNA expression profile was downloaded from the Gene Expression Omnibus (GEO) database. The RNA expression profile and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database. Difference analysis was conducted after quality control. Based on CircInteractome, TargetScan, and miRDB databases, a circRNA-related ceRNA network was constructed. R package "clusterProfiler" was used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Then, a univariate and multivariate Cox regression was used to construct a prognostic-related gene model to predict survival models. Finally, a gene set enrichment analysis (GSEA) analysis was performed to elucidate the function of genes related to prognosis. Altogether, 23 DEcircRNAs, 319 DEmiRNAs, and 14,541 DEmRNAs were identified. Based on ceRNA trends, the ceRNA network included 15 DEcircRNAs, 25 DEmiRNAs, and 1099 DEmRNAs in GC. Univariate and multivariate Cox proportional hazards regression analysis was used to establish a survival model with 11 prognosis-related genes and its AUC was 0.741, indicating good sensitivity and specificity in the prediction of GC prognosis. Finally, three prognostic-related genes were selected randomly to verify expression levels, which were consistent with the analysis result. The prognostic genes were significantly enriched in cancer-related biological processes, suggesting their roles in the onset and progression of GC. Our study constructs a prognostic model of GC, deepens our understanding of circRNA-related ceRNA networks in GC biology, and provided further implications for the diagnosis and treatment of GC.
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http://dx.doi.org/10.1038/s41417-020-00270-9DOI Listing
January 2021

Berberine Prevents Disease Progression of Nonalcoholic Steatohepatitis through Modulating Multiple Pathways.

Cells 2021 Jan 21;10(2). Epub 2021 Jan 21.

Department of Microbiology and Immunology, Medical College of Virginia and McGuire Veterans Affairs Medical Center, Virginia Commonwealth University, 1220 East Broad Street, MMRB-5044, Richmond, VA 23298, USA.

The disease progression of nonalcoholic fatty liver disease (NAFLD) from simple steatosis (NAFL) to nonalcoholic steatohepatitis (NASH) is driven by multiple factors. Berberine (BBR) is an ancient Chinese medicine and has various beneficial effects on metabolic diseases, including NAFLD/NASH. However, the underlying mechanisms remain incompletely understood due to the limitation of the NASH animal models used. A high-fat and high-fructose diet-induced mouse model of NAFLD, the best available preclinical NASH mouse model, was used. RNAseq, histological, and metabolic pathway analyses were used to identify the potential signaling pathways modulated by BBR. LC-MS was used to measure bile acid levels in the serum and liver. The real-time RT-PCR and Western blot analysis were used to validate the RNAseq data. BBR not only significantly reduced hepatic lipid accumulation by modulating fatty acid synthesis and metabolism but also restored the bile acid homeostasis by targeting multiple pathways. In addition, BBR markedly inhibited inflammation by reducing immune cell infiltration and inhibition of neutrophil activation and inflammatory gene expression. Furthermore, BBR was able to inhibit hepatic fibrosis by modulating the expression of multiple genes involved in hepatic stellate cell activation and cholangiocyte proliferation. Consistent with our previous findings, BBR's beneficial effects are linked with the downregulation of microRNA34a and long noncoding RNA H19, which are two important players in promoting NASH progression and liver fibrosis. : BBR is a promising therapeutic agent for NASH by targeting multiple pathways. These results provide a strong foundation for a future clinical investigation.
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http://dx.doi.org/10.3390/cells10020210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912096PMC
January 2021

Nanosecond optical parametric oscillator with midinfrared intracavity difference-frequency mixing in orientation-patterned GaAs.

Opt Lett 2021 Jan;46(2):332-335

We report on efficient midinfrared difference-frequency generation (DFG) in orientation-patterned GaAs by intracavity mixing the signal and idler pulses of a narrowband nanosecond optical parametric oscillator based on periodically poled . The maximum average DFG output power reached 215 mW at 8.15 µm for a repetition rate of 35 kHz. The temperature tuning range spanned over 8026-8710 nm. The maximum overall conversion efficiency from 1 to 8 µm amounted to ∼1.3.
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http://dx.doi.org/10.1364/OL.413583DOI Listing
January 2021

A novel antisense long noncoding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum.

Environ Microbiol 2021 Jan 12. Epub 2021 Jan 12.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Fusarium graminearum is an important worldwide pathogen that causes Fusarium head blight (FHB) in wheat, barley, maize, and other grains. LncRNAs play important roles in many biological processes, but little is known about their functions and mechanisms in filamentous fungi. Here, we report that a natural antisense RNA, GzmetE-AS, is transcribed from the opposite strand of GzmetE. GzmetE encodes a homoserine O-acetyltransferase, which is important for sexual development and plant infection. The expression of GzmetE-AS was increased significantly during the conidiation stage, while GzmetE was upregulated in the late stage of sexual reproduction. Overexpression of GzmetE-AS inhibited the transcription of GzmetE. In contrast, the expression of GzmetE was significantly increased in GzmetE-AS transcription termination strain GzmetE-AS-T. Furthermore, GzmetE-AS-T produced more perithecia and facilitated the ascospore discharge, resembling the phenotype of GzmetE overexpressing strains. However, overexpression of GzmetE-AS in ∆dcl1/2 strain cannot inhibit the expression of GzmetE, and the GzmetE nat-siRNA is also significantly reduced in ∆dcl1/2 mutant. Taken together, we have identified a novel antisense lncRNA GzmetE-AS, which is involved in asexual and sexual reproduction by regulating its antisense gene GzmetE through RNAi pathway. Our findings reveal that the lncRNA plays critical roles in the development in F. graminearum. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1462-2920.15399DOI Listing
January 2021

Isoflavones' effects on pharmacokinetic profiles of main iridoids from in rats.

J Pharm Anal 2020 Dec 14;10(6):571-580. Epub 2019 Nov 14.

School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.

(GF) and Praeparatum (SSP) are both medicine food homologies and widely used in Chinese clinical prescriptions together. The research investigated the pharmacokinetics of four iridoids in normal rats and isolfavones-fed rats, which were administered with isolfavones from SSP for 7, 14, 21 and 28 consecutive days. A validated LC-MS/MS method was developed for determining shanzhiside, genipin-1-gentiobioside, geniposide and their metabolite genipin in rat plasma. Plasma samples were pretreated by solid-phase extraction using paeoniflorin as the internal standard. The chromatographic separation was performed on a Waters Atlantis T3 (4.6 mm × 150 mm, 3 μm) column using a gradient mobile phase consisting of acetonitril and water (containing 0.06% acetic acid). The mass detection was under the multiple reaction monitoring (MRM) mode via polarity switching between negative and positive ionization modes. The calibration curves exhibited good linearity ( > 0.997) for all components. The lower limit of quantitation was in the range of 1-10 ng/mL. The intra-day and inter-day precisions (RSD) at three different levels were both less than 12.2% and the accuracies (RE) ranged from -10.1% to 16.4%. The extraction recovery of them ranged from 53.8% to 99.7%. Pharmacokinetic results indicated the bioavailability of three iridoid glycosides and the metabolite, genipin in normal rats was higher than that in rats exposed to isoflavones. With the longer time of administration of isoflavones, plasma concentrations of iridoids decreased, while genipin sulfate, the phase Ⅱ metabolite of genposide and genipin-1-gentiobioside, appeared the rising exposure. The pharmacokinetic profiles of main iridoids from GF were altered by isoflavones.
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http://dx.doi.org/10.1016/j.jpha.2019.11.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775847PMC
December 2020

Single-cell analysis reveals transcriptomic remodellings in distinct cell types that contribute to human prostate cancer progression.

Nat Cell Biol 2021 01 8;23(1):87-98. Epub 2021 Jan 8.

Department of Urology, Shanghai Changhai Hospital, Shanghai, P. R. China.

Prostate cancer shows remarkable clinical heterogeneity, which manifests in spatial and clonal genomic diversity. By contrast, the transcriptomic heterogeneity of prostate tumours is poorly understood. Here we have profiled the transcriptomes of 36,424 single cells from 13 prostate tumours and identified the epithelial cells underlying disease aggressiveness. The tumour microenvironment (TME) showed activation of multiple progression-associated transcriptomic programs. Notably, we observed promiscuous KLK3 expression and validated the ability of cancer cells in altering T-cell transcriptomes. Profiling of a primary tumour and two matched lymph nodes provided evidence that KLK3 ectopic expression is associated with micrometastases. Close cell-cell communication exists among cells. We identified an endothelial subset harbouring active communication (activated endothelial cells, aECs) with tumour cells. Together with sequencing of an additional 11 samples, we showed that aECs are enriched in castration-resistant prostate cancer and promote cancer cell invasion. Finally, we created a user-friendly web interface for users to explore the sequenced data.
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http://dx.doi.org/10.1038/s41556-020-00613-6DOI Listing
January 2021

Development of a 443 nm diode laser-based differential photoacoustic spectrometer for simultaneous measurements of aerosol absorption and NO.

Photoacoustics 2021 Mar 9;21:100229. Epub 2020 Dec 9.

School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230031, China.

Measurement of ambient aerosol and nitrogen dioxide (NO) is important as they are major pollutants from the burning of fossil fuel and biomass. In the present work, a differential photoacoustic spectrometer (D-PAS) was developed for simultaneous, online measurements of aerosol optical absorption and NO concentration. A novel photoacoustic resonator was designed and employed in the D-PAS for controlling a large flow rate, improving response time, and keeping the flow noise at a low level. The detection limits of 1.0 Mm and 0.87 ppb for aerosol absorption and NO concentration measurements were achieved with a lock-in amplifier time constant of 1 s. The D-PAS accuracy was demonstrated by performing a long-time, continuous measurement of aerosol, and NO in ambient air. The measured results of NO are consistent with the NOx analyzer and environmental monitoring station results.
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http://dx.doi.org/10.1016/j.pacs.2020.100229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749428PMC
March 2021

Continuous-wave and SESAM mode-locked operation of the Yb:BiSiO laser.

Opt Express 2021 Jan;29(1):105-110

We demonstrate a comprehensive characterization of the diode-pumped Yb:BiSiO laser operating in the continuous-wave and soliton mode-locked regimes. Pumping with a 650 mW, single-transverse mode, fiber-coupled laser diode, a maximum continuous-wave output power amounted to 213 mW with a slope efficiency up to 57.6%. A broadband wavelength tuning range of more than 70 nm was achieved in CW regime with a fused silica prism. Applying a SESAM as mode locker, nearly transform-limited pulses as short as 113 fs were generated for a maximum average power of 53 mW and a pulse repetition rate of ∼106 MHz. To the best of our knowledge, this is the first report on passively mode-locked operation with the Yb:BiSiO crystal.
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http://dx.doi.org/10.1364/OE.413930DOI Listing
January 2021

SESAM mode-locked Tm:LuYO ceramic laser generating 54-fs pulses at 2048  nm.

Appl Opt 2020 Nov;59(33):10493-10497

Mode-locked laser operation near 2.05 µm based on a mixed sesquioxide : ceramic is demonstrated. Continuous-wave and wavelength-tunable operation is also investigated. Employing a GaSb-based semiconductor saturable absorber mirror as a saturable absorber, a maximum average output power of 133 mW is obtained for a pulse duration of 59 fs. Pulses as short as 54 fs, i.e., eight optical cycles are generated at a repetition rate of ∼78 with an average output power of 51 mW. To the best of our knowledge, this result represents the shortest pulse duration ever achieved from Tm-based solid-state mode-locked lasers.
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http://dx.doi.org/10.1364/AO.408650DOI Listing
November 2020

Single-walled carbon-nanotube saturable absorber assisted Kerr-lens mode-locked Tm:MgWO laser.

Opt Lett 2020 Nov;45(22):6142-6145

We demonstrate sub-100-fs Kerr-lens mode-locking of a : laser emitting at ∼2µ assisted by a single-walled carbon-nanotube saturable absorber. A maximum average output power of 100 mW is achieved with pulse duration of 89 fs at a pulse repetition rate of ∼86. The shortest pulse duration derived from frequency-resolved optical gating amounts to 76 fs at 2037 nm, corresponding to nearly bandwidth-limited pulses. To the best of our knowledge, these are the shortest pulses generated from any Tm-doped tungstate crystal and the first report on saturable absorber assisted Kerr-lens mode-locking of a Tm laser at ∼2µ.
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http://dx.doi.org/10.1364/OL.411288DOI Listing
November 2020

Coherent combination of two intracavity eigenmodes producing linearly polarized emission in an isotropic laser.

Opt Express 2020 Nov;28(23):34337-34345

The behavior of directly linearly polarized emission was investigated in an isotropic Nd:YAG laser with a weakly anisotropic Fabry-Perot cavity. In order to explore the polarization mechanism, the intracavity eigenmodes were analyzed theoretically. A theoretical model was developed and shown to be suitable for describing the orientation-dependent beam profiles of the polarizer-specific features observed in experiments. This model indicates that the linear polarization emission results from the coherently combined state of intracavity eigenmodes as opposed to an intrinsic polarization state. The coherent combination is attributed to the frequency degeneracy and spontaneous phase locking of polarized eigenmodes. For the first time, the polarization coupling mechanism based on the coherent combination of eigenmodes in an isotropic solid-state laser was demonstrated. Moreover, this study also contributes an effective method to judge different types of linear polarization states.
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http://dx.doi.org/10.1364/OE.405813DOI Listing
November 2020

UPLC/MS-based untargeted metabolomics reveals the changes of metabolites profile of Salvia miltiorrhiza bunge during Sweating processing.

Sci Rep 2020 11 11;10(1):19524. Epub 2020 Nov 11.

College of Pharmacy, Anhui University of Chinese Medicine, Room 405, Qianjiang Road No. 1, Yaohai Disrict, Hefei, 230012, Anhui Province, China.

Salvia miltiorrhiza has numerous compounds with extensive clinical application. "Sweating", a processing method of Traditional Chinese Medicine (TCM), results in great changes in pharmacology and pharmacodynamics. Previously, chromatogram of 10 characteristic metabolites in S. miltiorrhiza showed a significant difference after "Sweating". Due to the complexity of TCM, changes in metabolites should be investigated metabolome-wide after "Sweating". An untargeted UPLC/MS-based metabolomics was performed to discover metabolites profile variation of S. miltiorrhiza after "Sweating". Multivariate analysis was conducted to screen differential metabolites. Analysis indicated distinct differences between sweated and non-sweated samples. 10,108 substance peaks had been detected altogether, and 4759 metabolites had been identified from negative and positive ion model. 287 differential metabolites were screened including 112 up-regulated and 175 down-regulated and they belong to lipids and lipid-like molecules, and phenylpropanoid and polyketides. KEGG analysis showed the pathway of linoleic acid metabolism, and glyoxylate and dicarboxylate metabolism were mainly enriched. 31 and 49 identified metabolites were exclusively detected in SSM and NSSM, respectively, which mainly belong to carboxylic acids and derivatives, polyketides and fatty acyls. By mapping tanshinones and salvianolic acids to 4759 identified metabolites library, 23 characteristic metabolites had been identified, among which 11 metabolites changed most. We conclude that "Sweating'' has significant effect on metabolites content and composition of S. miltiorrhiza.
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http://dx.doi.org/10.1038/s41598-020-76650-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658355PMC
November 2020

The influencing factors and spillover effects of interprovincial agricultural carbon emissions in China.

PLoS One 2020 4;15(11):e0240800. Epub 2020 Nov 4.

School of Architecture, Tianjin University, Tianjin, China.

Agricultural carbon emissions have become the constraints of agricultural low-carbon and circular economy development in China. China's agricultural production faces severe pressures and challenges in agricultural carbon reduction. In this paper, we take observation for the 31 provinces in china from 1997 to 2017, to explore the influencing factors and spatial spillover effects of agricultural by estimating spatial panel data models. The results show that China's agricultural carbon emissions will continue to increase in the future, because the growth of per capita gross domestic product (GDP) is the main driving force to accelerate the growth of agricultural carbon emissions, but the agricultural input factors will help to reduce the growth of carbon emissions. Moreover, it is proved that economic factors and agricultural input factors have direct effects and spatial spillover effects on agricultural carbon emissions except for agricultural environmental factors. In the short term, strengthening environmental protection may bring some pressure to the economic development of some places, but to achieve high-quality development, we must fundamentally solve the problem of environmental pollution. The conclusion provides important enlightenment and scientific basis for formulating effective policies to curb the growth of CO2 emissions in China.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0240800PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641402PMC
December 2020

A Dual-Laser Sensor Based on Off-Axis Integrated Cavity Output Spectroscopy and Time-Division Multiplexing Method.

Sensors (Basel) 2020 Oct 30;20(21). Epub 2020 Oct 30.

Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Anhui 230031, China.

In this article, a compact dual-laser sensor based on an off-axis integrated-cavity output spectroscopy and time-division multiplexing method is reported. A complete dual-channel optical structure is developed and integrated on an optical cavity, which allows two distributed feedback (DFB) lasers operating at wavelengths of 1603 nm and 1651 nm to measure the concentration of CO and CH, simultaneously. Performances of the dual-laser sensor are experimentally evaluated by using standard air (with a mixture of CO and CH). The limit of detection (LoD) is 0.271 ppm and 1.743 ppb at a 20 s for CO and CH, respectively, and the noise equivalent absorption sensitivities are 2.68 × 10 cm Hz and 3.88 × 10 cm Hz, respectively. Together with a commercial instrument, the dual-laser sensor is used to measure CO and CH concentration over 120 h and verify the regular operation of the sensor for the detection of ambient air. Furthermore, a first-order exponential moving average algorithm is implemented as an effective digital filtering method to estimate the gas concentration.
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http://dx.doi.org/10.3390/s20216192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662223PMC
October 2020

The D-galacturonic acid catabolic pathway genes differentially regulate virulence and salinity response in Sclerotinia sclerotiorum.

Fungal Genet Biol 2020 Dec 31;145:103482. Epub 2020 Oct 31.

Department of Plant Pathology, Washington State University, Pullman, WA 99164, USA; USDA Agricultural Research Service, Pullman, WA 99164, USA. Electronic address:

Sclerotinia sclerotiorum causes white mold disease on a wide range of economically important crops such as soybean, canola, tomato, pea and sunflower. As one of the most successful plant pathogens, S. sclerotiorum has the unique ability of adapting to various environmental conditions and effectively suppressing or evading plant defense. Notably, S. sclerotiorum secretes an array of plant cell-wall degrading enzymes (CWDEs) to macerate host cell wall and utilizes the liberated monosaccharides and oligosaccharides as nutrients. One of the major plant cell wall constituents is polygalacturonic acid in pectin, with D-galacturonic acid being the most abundant component. In this research, we identified four S. sclerotiorum genes that encode the enzymes for the D-galacturonic acid catabolism, namely Ssgar1, Ssgar2, Sslgd1 and Sslga1. Gene-knockout mutants were created for all four catabolic genes. When cultured on pectin as the alternative carbon source, Sslgd1- and Sslga1-deletion mutants and Ssgar1/Ssgar2 double deletion mutants exhibited significantly reduced growth. The D-galacturonic acid catabolic genes are transcriptionally induced by either polygalacturonic acid in the culture media or during host infection. Virulence tests of the knockout mutants revealed that Ssgar2, Sslgd1 and Sslga1 all facilitated the effective colonization of S. sclerotiorum to the leaves of soybean and pea, but not of tomato which has the lowest D-galacturonic acid contents in its leaves. In addition to their positive roles in virulence, all four enzymes negatively affect S. sclerotiorum tolerance to salt stress. SsGAR2 has an additional function in tolerance to Congo Red, suggesting a potential role in cell wall stability of S. sclerotiorum. This study is the first report revealing the versatile functions of D-galacturonic acid catabolic genes in S. sclerotiorum virulence, salinity response and cell wall integrity.
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http://dx.doi.org/10.1016/j.fgb.2020.103482DOI Listing
December 2020

Diet supplemented with fermented okara improved growth performance, meat quality, and amino acid profiles in growing pigs.

Food Sci Nutr 2020 Oct 7;8(10):5650-5659. Epub 2020 Sep 7.

State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture and Rural Affairs Guangdong Key Laboratory of Animal Breeding and Nutrition Guangdong Engineering Technology Research Center of animal Meat quality and Safety Control and Evaluation Institute of Animal Science Guangdong Academy of Agricultural Sciences Guangzhou China.

This study aimed to assess the efficacy of fermented okara on performance and meat quality, and to explore the feasibility of its partial substitution for corn-soybean meal in pig production. A total of 48 pigs (Duroc × Landrace × Yorkshire) with an average body weight of 58.60 ± 0.65 kg were randomly assigned to 2 groups, Control group and Fermented okara (FO) group. There were 8 replicate pens each with 3 pigs per treatment. Control pigs were fed a corn-soybean meal basal diet, treatment pigs were fed a basal diet supplemented with FO throughout the 55-d experimental period. Results showed that fermentation of okara using probiotics increased its microporous structure, polysaccharides, lactic acid, and free amino acids (FAA) by 46.06%, 150%, and 66.45% compared with unfermented okara, respectively ( < .05). The diet supplemented with FO significantly improved average daily gain (ADG) by 8.70% ( < .01), but decreased the feed gain ratio (F/G) by 5.56% of growing pigs compared to the control diet ( < .05). Furthermore, dietary FO improve meat color, FAA, and the activity of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX) in the serum and muscles ( < .05). Collectively, probiotics-fermented okara improved growth performance, meat quality and antioxidant capacity, and it can be used to substitute partial corn-soybean meal in pig industry.
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http://dx.doi.org/10.1002/fsn3.1857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590273PMC
October 2020

Chickpea seed rot and damping-off caused by metalaxyl-resistant Pythium ultimum and its management with ethaboxam.

Plant Dis 2020 Oct 29. Epub 2020 Oct 29.

USDA-ARS, Grain Legume genetics and Physiology, Pullman, Washington, United States.

Metalaxyl and its isomer mefenoxam have been the primary fungicides used as seed treatments in managing Pythium seed rot and damping-off of chickpea. However, recent outbreaks of seed rot and damping-off of metalaxyl-treated chickpea seeds were found in the dryland agriculture regions of southeastern Washington and northern Idaho. Pythium spp. isolated from rotten seeds and associated soils showed high levels of resistance to metalaxyl. Large proportions (31 to 91%) of Pythium isolates resistant to metalaxyl were detected in areas where severe chickpea damping-off occurred and were observed in commercial chickpea fields over several years. All metalaxyl-resistant isolates were identified as P. ultimum var. ultimum. The metalaxyl resistance trait measured by EC50 values was stable over 10 generations in the absence of metalaxyl, and no observable fitness costs were associated with metalaxyl resistance. Under controlled conditions, metalaxyl treatments failed to protect chickpea seeds from seed rot and damping-off following inoculation with metalaxyl-resistant Pythium isolates. In culture, ethaboxam inhibited mycelial growth of metalaxyl-resistant, as well as metalaxyl-sensitive isolates. Greenhouse and field tests showed that ethaboxam is effective in managing metalaxyl-resistant Pythium. Ethaboxam in combination with metalaxyl is now commonly applied as seed treatments in commercial chickpea production.
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http://dx.doi.org/10.1094/PDIS-08-20-1659-REDOI Listing
October 2020

Investigation of catalysts M/CeO (M = Pt, Rh, or Pd) for purification of CO derived from oxycombustion in the absence or presence of water.

Environ Sci Pollut Res Int 2021 Mar 20;28(10):12521-12532. Epub 2020 Oct 20.

SFR Condorcet FR CNRS 3417, Univ. Littoral Côte d'Opale, Unité de Chimie Environnementale et Interactions sur le Vivant, UR 4492, 145 avenue Maurice Schumann, Dunkerque, 59140, France.

Oxyfuel combustion is a promising technology to produce a CO-rich flue gas ready suitable for sequestration or valorization. But its storage as well as its further valorization requires to increase the CO purification as a small amount of CO and NOx are produced during combustion. Based on the technology developed for three-way converters, similar systems, i.e., M/CeO where M is Pt, Pd, or Rh, were studied for NO-CO abatement in a gas stream similar to those obtained when an oxyfuel combustion is performed. The results evidenced that the role of the metal nature influences the performances obtained on NO-CO abatement, platinum supported on ceria being the most efficient catalyst. We also measured the impact of the presence of water in the reaction stream on the catalytic activity of these materials. It appears that the presence of water has a beneficial effect on the different reactions due to a water gas shift reaction that increases the reduction of the NO and favors the formation of N. The study pointed out that platinum supported on ceria remained the best catalyst, under these wet operating conditions close to industrial ones, for purification of oxyfuel combustion exhausts.
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http://dx.doi.org/10.1007/s11356-020-11252-7DOI Listing
March 2021

Safety and Feasibility of Robotic Natural Orifice Specimen Extraction Surgery in Colorectal Neoplasms During the Initial Learning Curve.

Front Oncol 2020 11;10:1355. Epub 2020 Sep 11.

Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.

To analyze the learning curve (LC) for robotic natural orifice specimen extraction surgery (NOSES) for colorectal neoplasms and evaluate safety and feasibility during the initial LC. Patients who consecutively underwent robotic NOSES performed by two surgeons between March 2016 and October 2019 were analyzed retrospectively. The operation time was evaluated using the cumulative sum method to analyze the LC. The clinicopathological data before and after the completion of LC were extracted and compared to evaluate safety and feasibility. In total, 99 and 66 cases were scheduled for robotic NOSES by Prof. Yao and Prof. Li, respectively. The peak points of LC were observed at the 42nd and 15th cases of Yao and Li, respectively, then operation time began to decrease. Only the operation time for Yao before the completion of LC (213.3 ± 67.0 min) was longer than that after the completion of LC (143.8 ± 33.3 min). For Yao nor for Li, other indices, such as postoperative hospital stay, intraoperative blood loss, conversion to laparotomy, incidence of anastomotic leakage, reoperation rate, and 90-day mortality rate lacked significant statistical differences( > 0.05). In terms of feasibility, the number of lymph nodes harvested, positive resection margin rate, and total cost before and after the completion of LC had no significant statistical difference ( > 0.05). The cases before the completion of LC for robotic NOSES in colorectal neoplasms varied from 15 cases to 42 cases. Robotic NOSES is safe and feasible during the initial LC.
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http://dx.doi.org/10.3389/fonc.2020.01355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533530PMC
September 2020

Uterine Metabolomics Reveals Protection of Taohong Siwu Decoction Against Abnormal Uterine Bleeding.

Front Pharmacol 2020 11;11:507113. Epub 2020 Sep 11.

AnHui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine, Hefei, China.

Incomplete abortion, a procedure for terminating pregnancy, will lead to abnormal uterine bleeding (AUB), infections, and even death. Taohong Siwu decoction (TSD) is a traditional Chinese medicine (TCM) formula, which has been developed to treat AUB for hundreds of years. However, the mechanism of the protective effect of TSD against AUB is not clear. We performed mass spectrometry (MS) of uterine samples to observe metabolic profile resulting from the treatment with TSD. An integrated gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry based untargeted metabolomics approach combined with multivariate statistical analyses were used to investigate the metabolic profile of TSD against AUB. There was clear separation between pregnant and incomplete aborting rats as well as incomplete aborting and TSD administered rats. Based on random forest algorithm and receiver operator characteristic analysis, 12 biomarkers were optimized related to TSD administered. The effect of TSD on AUB are related to several pathways, such as AA metabolism, glyoxylate and dicarboxylate metabolism, alanine, aspartate, and glutamate metabolism. To our knowledge, this is the first uterine metabolomics study focusing on TSD on AUB and provide a new perspective for explaining the mechanism of TSD on AUB.
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http://dx.doi.org/10.3389/fphar.2020.507113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518030PMC
September 2020

Outbreaks of Highly Pathogenic Avian Influenza (H5N6) Virus Subclade 2.3.4.4h in Swans, Xinjiang, Western China, 2020.

Emerg Infect Dis 2020 12 8;26(12):2956-2960. Epub 2020 Oct 8.

In January 2020, the subclade 2.3.4.4h of highly pathogenic avian influenza (H5N6) virus infected migratory whooper swans and mute swans in Xinjiang, western China. The virus is lethal to chickens and ducks but has low pathogenicity in mice. Antigenically, this subclade is similar to the H5N1 vaccine seed virus Re-11.
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http://dx.doi.org/10.3201/eid2612.201201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706961PMC
December 2020

Effect of Long-term Transcranial Direct Current Stimulation on Glx and GABA: A Pilot Study.

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:3561-3564

Previous studies have demonstrated that transcranial direct current stimulation (tDCS) over the dorsolateral prefrontal cortex (dlPFC) can enhance working memory. However, the mechanism underlying the long-term tDCS is still unclear. This pilot study aims to examine neurotransmitters such as gamma-aminobutyric (GABA) and Glx (a measure of glutamate and glutamine combined) and working memory in response to the long-term anodal tDCS over dlPFC. Six healthy, right-handed young adults enrolled in this study received 2-mA anodal tDCS over dlPFC within 4 weeks. Long-term tDCS means that it was applied 5 times per week for the first two weeks and once for the last two weeks with 30 min each time. The other six participants were enrolled as the control group without stimulation for testing the baseline enhancement of working memory due to learning. The GABA and Glx levels were assessed by Magnetic Resonance Spectroscopy (MRS), while a 3-back task was performed to assess working memory. Data were collected at the beginning of the experiment, after two-week tDCS and at the end of the experiment. We found that the working memory was not significantly enhanced by the first two-week tDCS because the accuracy of response in 3-back was not significantly increased compared to the control group. Meanwhile, there were no significant changes in the levels of GABA. However, the Glx level was found significantly decreased in both 2- and 4-week MRS measurements. The observation that the long-term tDCS causes the decrease of excitatory neurotransmitters implies the different underlying mechanisms between the long-term tDCS and the single one.
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http://dx.doi.org/10.1109/EMBC44109.2020.9175996DOI Listing
July 2020

Orthogonalizing the Activity of Two Neural Units for 2D Cursor Movement Control.

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:3046-3049

In the design of brain-machine interface (BMI), as the number of electrodes used to collect neural spike signals declines slowly, it is important to be able to decode with fewer units. We tried to train a monkey to control a cursor to perform a two-dimensional (2D) center-out task smoothly with spiking activities only from two units (direct units). At the same time, we studied how the direct units did change their tuning to the preferred direction during BMI training and tried to explore the underlying mechanism of how the monkey learned to control the cursor with their neural signals. In this study, we observed that both direct units slowly changed their preferred directions during BMI learning. Although the initial angles between the preferred directions of 3 pairs units are different, the angle between their preferred directions approached 90 degrees at the end of the training. Our results imply that BMI learning made the two units independent of each other. To our knowledge, it is the first time to demonstrate that only two units could be used to control a 2D cursor movements. Meanwhile, orthogonalizing the activities of two units driven by BMI learning in this study implies that the plasticity of the motor cortex is capable of providing an efficient strategy for motor control.
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http://dx.doi.org/10.1109/EMBC44109.2020.9175931DOI Listing
July 2020

lncRNA TINCR facilities bladder cancer progression via regulating miR‑7 and mTOR.

Mol Med Rep 2020 Nov 22;22(5):4243-4253. Epub 2020 Sep 22.

Shengli Hospital of Shengli Petroleum Administration, Dongying, Shandong 257055, P.R. China.

Long non‑coding RNAs (lncRNAs) have been implicated in various human malignancies, but the molecular mechanism of lncRNA TINCR ubiquitin domain containing (TINCR) in bladder cancer remains unclear. The present study found that the expression of TINCR was significantly increased in bladder cancer tissues and cell lines, when compared with that in adjacent normal tissues and normal urinary tract epithelial cell line SV‑HUC‑1, respectively. Moreover, the high expression of TINCR was associated with tumor metastasis and advanced tumor, node, metastasis stage, as well as reduced overall survival rates of patients with bladder cancer. Further investigation revealed that microRNA (miR)‑7 was negatively mediated by TINCR in bladder cancer cells. Silencing of TINCR expression significantly increased miR‑7 expression and reduced bladder cancer cell proliferation, migration and invasion, while knockdown of miR‑7 expression reversed the inhibitory effects of TINCR downregulation on bladder cancer cells. mTOR was then identified as a target gene of miR‑7 in bladder cancer, and it was demonstrated that overexpression of mTOR reversed the inhibitory effects of miR‑7 on bladder cancer cells. In conclusion, this study suggests that TINCR/miR‑7/mTOR signaling may be a potential therapeutic target for bladder cancer.
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http://dx.doi.org/10.3892/mmr.2020.11530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533511PMC
November 2020

Analysis of the active components and metabolites of Taohong Siwu decoction by using ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry.

J Sep Sci 2020 Nov 30;43(22):4131-4147. Epub 2020 Sep 30.

Anhui Province Key Laboratory of Chinese Medicinal Formula, Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Anhui University of Chinese Medicine, Hefei, P. R. China.

Taohong Siwu Decoction is a classic Chinese medicine prescription for treatment of cerebral ischemia and gynecological diseases. However, the active ingredients of Taohong Siwu Decoction have not been identified. In this study, a ultra performance liquid chromatography quadrupole time-of-flight mass system was used to analyze the active components and metabolites of Taohong Siwu Decoction absorbed into the blood and the brain. A total of 39 active compounds and 90 metabolites were identified in the blood and brain by comparing retention times, accurate masses, fragmentation patterns, and literature data. The results showed that flavonoids (Carthamus tinctorius L), aromatic organic acids, and benzoquinones (Angelica sinensis (Oliv.) Diels and Ligusticumchuanxiong hort) were prominent active ingredients in Taohong Siwu Decoction. Furthermore, hydrolysis, glucuronidation, and sulfation were identified as the main metabolic pathways of Taohong Siwu Decoction in vivo. This was the first study to characterize the active components and metabolites of Taohong Siwu Decoction in the blood and brain using ultra performance liquid chromatography quadrupole time-of-flight mass.
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http://dx.doi.org/10.1002/jssc.202000498DOI Listing
November 2020

Unnatural Amino-Acid-Based Star-Shaped Poly(l-Ornithine)s as Emerging Long-Term and Biofilm-Disrupting Antimicrobial Peptides to Treat Pseudomonas aeruginosa-Infected Burn Wounds.

Adv Healthc Mater 2020 10 6;9(19):e2000647. Epub 2020 Sep 6.

Department of Chemistry, University of South Florida, Tampa, FL, 33620, USA.

Peptide-based antimicrobial materials are recognized as promising alternatives to antibiotics to circumvent the emergence of antibiotic-resistant bacteria or to combat multiple resistant bacteria by targeting the bacterial cell membrane. The components and conformations of antimicrobial peptides are extensively explored to achieve broad-spectrum and effective antimicrobial activity. Here, star-shaped antimicrobial polypeptides are fabricated by employing homologs of poly(l-lysine)s (i.e., poly(l-ornithine)s, poly(l-lysine)s, and poly(l-α,ζ-diaminoheptylic acid)s) with the aim of modulating their charge/hydrophobicity balance and rationalizing their structure-antimicrobial property relationships. The in vitro antibacterial investigation reveals that unnatural amino-acid-based star-shaped poly(l-ornithine)s have remarkable proteolytic stability, excellent biofilm-disrupting capacity, and broad-spectrum antimicrobial activity, even against difficult-to-kill Gram-negative Pseudomonas aeruginosa. Furthermore, star-shaped poly(l-ornithine)s significantly reduce the microbial burden and improve the burn wound healing of mouse skin infected with P. aeruginosa. These results demonstrate that unnatural amino-acid-based star-shaped poly(l-ornithine)s can serve as emerging long-term and biofilm-disrupting antimicrobial agents to treat biofilm-related infections in burn, especially caused by notorious P. aeruginosa.
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http://dx.doi.org/10.1002/adhm.202000647DOI Listing
October 2020