Publications by authors named "WeiXia Li"

63 Publications

Differentiation of Hepatocellular Carcinoma from Hepatic Hemangioma and Focal Nodular Hyperplasia using Computed Tomographic Spectral Imaging.

J Clin Transl Hepatol 2021 Jun 31;9(3):315-323. Epub 2021 Mar 31.

Department of Radiology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background And Aims: Hepatocellular carcinoma (HCC) is the most common primary hepatic malignancy. This study was designed to investigate the value of computed tomography (CT) spectral imaging in differentiating HCC from hepatic hemangioma (HH) and focal nodular hyperplasia (FNH).

Methods: This was a retrospective study of 51 patients who underwent spectral multiple-phase CT at 40-140 keV during the arterial phase (AP) and portal venous phase (PP). Slopes of the spectral curves, iodine density, water density derived from iodine- and water-based material decomposition images, iodine uptake ratio (IUR), normalized iodine concentration, and the ratio of iodine concentration in liver lesions between AP and PP were measured or calculated.

Results: As energy level decreased, the CT values of HCC (=31), HH (=17), and FNH (=7) increased in both AP and PP. There were significant differences in IUR in the AP, IUR in the PP, normalized iodine concentration in the AP, slope in the AP, and slope in the PP among HCC, HH, and FNH. The CT values in AP, IUR in the AP and PP, normalized iodine concentration in the AP, slope in the AP and PP had high sensitivity and specificity in differentiating HH and HCC from FNH. Quantitative CT spectral data had higher sensitivity and specificity than conventional qualitative CT image analysis during the combined phases.

Conclusions: Mean CT values at low energy (40-90 keV) and quantitative analysis of CT spectral data (IUR in the AP) could be helpful in the differentiation of HCC, HH, and FNH.
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http://dx.doi.org/10.14218/JCTH.2020.00173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237149PMC
June 2021

A Novel Prediction Model of COVID-19 Progression: A Retrospective Cohort Study.

Infect Dis Ther 2021 Jun 14. Epub 2021 Jun 14.

Department of Liver Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.

Introduction: Estimating the risk of disease progression is of utmost importance for planning appropriate setting of care and treatment for patients with coronavirus disease 2019 (COVID-19). This study aimed to develop and validate a novel prediction model of COVID-19 progression.

Methods: In total, 814 patients in the training set were included to develop a novel scoring system; and 420 patients in the validation set were included to validate the model.

Results: A prediction score, called ACCCDL, was developed on the basis of six risk factors associated with COVID-19 progression: age, comorbidity, CD4 T cell count, C-reactive protein (CRP), D-dimer, and lactate dehydrogenase (LDH). For predicting COVID-19 progression, the ACCCDL score yielded a significantly higher area under the receiver operating characteristic curve (AUROC) compared with the CALL score, CoLACD score, PH-COVID-19 score, neutrophil-lymphocyte ratio, and lymphocyte-monocyte ratio both in the training set (0.92, 0.84, 0.83, 0.83, 0.76, and 0.65, respectively) and in the validation set (0.97, 0.83, 0.83, 0.78, 0.74, and 0.60, respectively). Over 99% of patients with the ACCCDL score < 12 points will not progress to severe cases, and over 30% of patients with the ACCCDL score > 20 points will progress to severe cases.

Conclusion: The ACCCDL score could stratify patients with at risk of COVID-19 progression, and was useful in regulating the large flow of patients with COVID-19 between primary health care and tertiary centers.
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http://dx.doi.org/10.1007/s40121-021-00460-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202540PMC
June 2021

Efficacy Evaluation of Thymosin Alpha 1 in Non-severe Patients With COVID-19: A Retrospective Cohort Study Based on Propensity Score Matching.

Front Med (Lausanne) 2021 23;8:664776. Epub 2021 Apr 23.

Department of Liver Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Thymosin alpha 1 (Thymosin-α1) is a potential treatment for patients with COVID-19. We aimed to determine the effect of Thymosin-α1 in non-severe patients with COVID-19. We retrospectively enrolled 1,388 non-severe patients with COVID-19. The primary and secondary clinical outcomes were evaluated with comparisons between patients treated with or without Thymosin-α1 therapy. Among 1,388 enrolled patients, 232 patients (16.7%) received both Thymosin-α1 therapy and standard therapy (Thymosin-α1 group), and 1,156 patients (83.3%) received standard therapy (control group). After propensity score matching (1:1 ratio), baseline characteristics were well-balanced between the Thymosin-α1 group and control group. The proportion of patients that progressed to severe COVID-19 is 2.17% for the Thymosin-α1 group and 2.71% for the control group ( = 0.736). The COVID-19-related mortality is 0.54% for the Thymosin-α1 group and 0 for the control group ( = 0.317). Compared with the control group, the Thymosin-α1 group had significantly shorter SARS-CoV-2 RNA shedding duration (13 vs. 16 days, = 0.025) and hospital stay (14 vs. 18 days, < 0.001). No statistically significant difference was found between the Thymosin-α1 group and control group in duration of symptoms (median, 4 vs. 3 days, = 0.843) and antibiotic utilization rate (14.1% vs. 15.2%, = 0.768). For non-severe patients with COVID-19, Thymosin-α1 can shorten viral RNA shedding duration and hospital stay but did not prevent COVID-19 progression and reduce COVID-19-related mortality rate.
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http://dx.doi.org/10.3389/fmed.2021.664776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102900PMC
April 2021

Stated acceptance and behavioral responses of drivers towards innovative connected vehicle applications.

Accid Anal Prev 2021 Jun 30;155:106095. Epub 2021 Mar 30.

Center for Environmental Research and Technology, University of California at Riverside, 1084 Columbia Ave, Riverside, CA, 92507, USA. Electronic address:

This research is aimed at investigating drivers' attitudes towards connected vehicle technology in general and two connected vehicle applications in particular-Lane Speed Monitoring and High Speed Differential Warning-which have been demonstrated via simulation to be effective in enhancing traffic mobility and safety, respectively. An online survey was sent to customers of an automobile manufacturer in the United States. Out of the 1453 survey responses that were received, 650 complete and valid responses were used to analyze the respondents' stated acceptance of and expected behavioral responses to the two connected vehicle applications under a variety of scenarios. Statistical analyses were conducted to examine the influence of demographic and socioeconomic factors. The results reveal that the respondents express high willingness to use connected vehicle technology and the two applications under various circumstances, and the willingness is strongly associated with age, gender, education level, and income. Higher levels of acceptance are observed in older, male, higher-educated, or higher-income respondents, while the patterns of conditional acceptance and expected behavioral responses vary with specific scenarios. These results provide useful information for application developers, traffic operators, and policy makers to steer connected vehicle technology development and deployment in the direction that will benefit both the users and the society.
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http://dx.doi.org/10.1016/j.aap.2021.106095DOI Listing
June 2021

A Novel Prediction Model for Long-Term SARS-CoV-2 RNA Shedding in Non-Severe Adult Hospitalized Patients with COVID-19: A Retrospective Cohort Study.

Infect Dis Ther 2021 Jun 31;10(2):897-909. Epub 2021 Mar 31.

Department of Liver Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.

Introduction: Due to the lack of clear direction (evidence) on the duration of viral shedding and thus potential for transmission, this retrospective study aimed to come up with a prediction model of prolonged coronavirus disease-19 (COVID-19) transmission or infection-spreading potential.

Methods: A total of 1211 non-severe patients with COVID-19 were retrospectively enrolled. Multivariate Cox regression was performed to identify the risk factors associated with long-term SARS-CoV-2 RNA shedding, and a prediction model was established.

Results: In the training set, 796 patients were divided into the long-term (> 21 days) group (n = 116, 14.6%) and the short-term (≤ 21 days) group (n = 680, 85.4%) based on their viral shedding duration. Multivariate analysis identified that age > 50 years, comorbidity, CD4-positive T-lymphocytes count (CD4 + T cell) ≤ 410 cells/ul, C-reactive protein (CRP) > 10 mg/L, and the corticosteroid use were independent risk factors for long-term SARS-CoV-2 RNA shedding. Incorporating the five risk factors, a prediction model, named as the CCCCA score, was established, and its area under the receiver operator characteristic curve (AUROC) was 0.87 in the training set and 0.83 in the validation set, respectively. In the validation set, using a cut-off of 8 points, we found sensitivity, specificity, positive predictive value, and negative predictive value of 51.7%, 92.2%, 33.3%, and 96.2%, respectively. Long-term SARS-CoV-2 RNA shedding increased from 14/370 (3.8%) in patients with CCCCA < 8 points to 15/45 (33.3%) in patients with CCCCA ≥ 8 points.

Conclusion: Using the CCCCA score, clinicians can identify patients with long-term SARS-CoV-2 RNA shedding.
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http://dx.doi.org/10.1007/s40121-021-00437-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011066PMC
June 2021

Feasibility of automatic detection of small hepatocellular carcinoma (≤2 cm) in cirrhotic liver based on pattern matching and deep learning.

Phys Med Biol 2021 Apr 16;66(8). Epub 2021 Apr 16.

Department of Radiology, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, People's Republic of China.

Early detection of hepatocellular carcinoma (HCC) is crucial for clinical management. Current studies have reported large HCC detections using automatic algorithms, but there is a lack of research on automatic detection of small HCCs (sHCCs). This study is to investigate the feasibility of automatic detection of sHCC (≤2 cm) based on pattern matching and deep learning (PM-DL) model.. A retrospective study included 5376 image sets from 56 cirrhosis patients (28 sHCC patients with 32 pathologically confirmed lesions and 28 non-HCC cirrhosis patients) in the training-validation cohort to build and validate the model through five-fold cross-validation. In addition, an external test cohort including 6144 image sets from 64 cirrhosis patients (32 sHCC patients with 38 lesions and 32 non-HCC cirrhosis patients) was applied to further verify the generalization ability of the model. The proposed PM-DL model consisted of three main steps: 3D co-registration and liver segmentation, screening of suspicious lesions on diffusion-weighted imaging images based on pattern matching algorithm, and identification/segmentation of sHCC lesions on dynamic contrast-enhanced images with convolutional neural network.The PM-DL model achieved a sensitivity of 89.74% and a positive predictive value of 85.00% in the external test cohort for per-lesion analysis. No significant difference was observed in volumes (= 0.13) and the largest sizes (= 0.89) between manually delineated and segmented lesions. The DICE coefficient reached 0.77 ± 0.16. Similar performances were identified in the validation cohort. Moreover, the PM-DL model outperformed Liver Imaging Reporting and Data System (LI-RADS) in sensitivity (probable HCCs: LR-5 or LR-4,= 0.18; definite HCCs: LR-5,< 0.001), with a similar high specificity for per-patient analysis.. The PM-DL model may be feasible for accurate automatic detection of sHCC in cirrhotic liver.
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http://dx.doi.org/10.1088/1361-6560/abf2f8DOI Listing
April 2021

Efficacy evaluation of intravenous immunoglobulin in non-severe patients with COVID-19: A retrospective cohort study based on propensity score matching.

Int J Infect Dis 2021 Apr 9;105:525-531. Epub 2021 Jan 9.

Department of Liver Disease, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China. Electronic address:

Objectives: At the present time, there is an absence of any proven effective antiviral therapy for patients with coronavirus disease 2019 (COVID-19). The aim of this study was to assess the efficacy of intravenous immunoglobulin (IVIG) in non-severe patients with COVID-19.

Methods: A retrospective study based on propensity score matching (PSM) was designed. Primary outcomes included the severity and mortality rates. Secondary outcomes included the duration of fever, virus clearance time, length of hospital stay, and use of antibiotics.

Results: A total of 639 non-severe patients with COVID-19 were enrolled. Forty-five patients received IVIG therapy and 594 received non-IVIG therapy. After PSM (1:2 ratio), the baseline characteristics were well balanced between the IVIG group (n = 45) and control group (n = 90). No statistically significant difference was found between the IVIG group and control group in the duration of fever (median 3 vs 3 days, p = 0.667), virus clearance time (median 11 vs 10 days, p = 0.288), length of hospital stay (median 14 vs 13 days, p = 0.469), or use of antibiotics (40% vs 38.9%, p = 0.901). Meanwhile, compared to the IVIG group, no more patients in the control group progressed to severe disease (3.3% vs 6.6%, p = 0.376) or died (0 vs 2.2%, p = 0.156).

Conclusions: In non-severe patients with COVID-19, no benefit was observed with IVIG therapy beyond standard therapy.
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http://dx.doi.org/10.1016/j.ijid.2021.01.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833031PMC
April 2021

Psoralidin, a major component of Psoraleae Fructus, induces inflammasome activation and idiosyncratic liver injury.

Int Immunopharmacol 2021 Mar 7;92:107352. Epub 2021 Jan 7.

China Military Institute of Chinese Materia, The Fifth Medical Centre, Chinese PLA General Hospital, Beijing 100039, China; Integrative Medical Center, The Fifth Medical Centre, Chinese PLA General Hospital, Beijing 100039, China. Electronic address:

Idiosyncratic drug-induced liver injury (IDILI) is a rare but potentially fatal disease that is unpredictable and independent of the dose of the drug. Increasing evidence suggests that the majority of IDILI cases are immune-mediated, and the aberrant activation of inflammasome plays a vital role in progression. Psoraleae Fructus (PF), a tonic Chinese medicine, has been able to cause IDILI, but the precise mechanism of hepatotoxicity remains unclear. In this study, eight bioactive compounds involved in PF-induced inflammasome activation were investigated. The results demonstrated that psoralidin activated the inflammasomes followed by secreting caspase-1 and interleukin 1β (IL-1β) in a dose-dependent manner. Interestingly, MCC950, a potent inhibitor of the NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, could not entirely suppress the psoralidin-induced inflammasome activation. Moreover, psoralidin significantly induced IL-1β maturation and caspase-1 activation in NLRP3-knockout bone marrow-derived macrophages (BMDMs), suggesting that psoralidin not only activates the NLRP3 inflammasome but also activates other types of inflammasomes. The results also demonstrated that psoralidin activated the inflammasomes by promoting the C-terminal caspase recruitment domain (ASC) oligomerization, and the production of mitochondrial reactive oxygen species (mtROS) is a decisive factor in psoralidin-induced inflammasome activation. Importantly, in vivo data revealed that psoralidin induced hepatic inflammation, increased aminotransferase activity and increased the production of IL-1β and tumor necrosis factor(TNF-α) in a susceptible mouse model of lipopolysaccharide (LPS)-mediated IDILI. In summary, these results confirmed that psoralidin causes IDILI by inducing inflammasome activation. The study suggests that psoralidin is a possible risk factor and is responsible for PF-induced IDILI.
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http://dx.doi.org/10.1016/j.intimp.2020.107352DOI Listing
March 2021

Novel aqueous biphasic system based on ionic liquid for the simultaneous extraction of seven active pharmaceutical ingredients in aquatic environment.

Environ Sci Pollut Res Int 2021 Apr 5;28(14):17853-17864. Epub 2021 Jan 5.

College of Standardization, China Jiliang University, Hangzhou, 310018, China.

Nonsteroidal anti-inflammatory drugs and antibiotics are classes of active pharmaceutical ingredients (APIs), which are continuously contaminating the ecosystem through various anthropogenic activities. Because of their pseudo-persistence in the aquatic environment and their potentially chronic effects on aquatic life, it is important to closely monitor their concentrations in the aquatic environment using a sensitive analytical method. Sustainable aqueous biphasic systems (ABSs) composed of ionic liquids and biodegradable organic salt (sodium malate) were proposed. The phase diagrams of the systems were firstly determined, and [N]Cl-based ABS was selected for the simultaneous extraction and preconcentration of seven APIs. With the developed ABS, extraction efficiencies of APIs close to 100% were obtained. For the developed method, limits of detection (LODs) of 45, 65, 76, 14, 60, 48, and 51 ng L were obtained for indomethacin, ibuprofen, diclofenac, naproxen, ketoprofen, flurbiprofen, and chloramphenicol, respectively, providing from 1216- to 1238-fold improvement as compared with the analysis without preconcentration. From an economic and environmental point of view, we can predict the prospects and competitive position of the method developed.
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http://dx.doi.org/10.1007/s11356-020-11751-7DOI Listing
April 2021

Efficacy and Biomarker Analysis of Camrelizumab in Combination with Apatinib in Patients with Advanced Nonsquamous NSCLC Previously Treated with Chemotherapy.

Clin Cancer Res 2021 Mar 15;27(5):1296-1304. Epub 2020 Dec 15.

Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University, Shanghai, P.R. China.

Purpose: Our preclinical work suggests that appropriate angiogenesis inhibition could potentiate PD-1/PD-L1 blockade via alleviating hypoxia, increasing infiltration of CD8 T cells and reducing recruitment of tumor-associated macrophages. We hereby conducted a clinical trial to evaluate this combination in pretreated patients with advanced non-small cell lung cancer (NSCLC).

Patients And Methods: The study included phase Ib apatinib dose-escalation and phase II expansion cohorts. Patients received apatinib at doses of 250-500 mg orally once daily, in combination with camrelizumab 200 mg intravenously every 2 weeks.

Results: From March 2017 to October 2018, 105 chemotherapy-pretreated patients with nonsquamous NSCLC were enrolled and received apatinib 250 mg (recommended phase II dose) and camrelizumab. Among them, one (1.0%) complete response, 28 (26.7%) partial responses, and 48 (45.7%) stable diseases were observed. In the efficacy-evaluable population ( = 94), objective response rate (ORR) was 30.9% [95% confidence interval (CI), 21.7-41.2]. The median progression-free survival was 5.7 months (95% CI, 4.5-8.8) and overall survival was 15.5 months (95% CI, 10.9-24.5). Efficacy of combination therapy was evident across all PD-L1 and tumor mutation burden subgroups, and appeared to be improved in patients with STK11/KEAP1 mutation (mutant vs. wild-type, ORR: 42.9% vs. 28.1%; 1-year survival rate: 85.1% vs. 53.1%). No unexpected adverse events were observed.

Conclusions: Combined apatinib and camrelizumab showed encouraging antitumor activity and acceptable toxicity in chemotherapy-pretreated patients with advanced nonsquamous NSCLC. Patients with STK11/KEAP1 mutation might derive more benefits from this combination. We will validate these results in an ongoing phase III trial (NCT04203485).
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http://dx.doi.org/10.1158/1078-0432.CCR-20-3136DOI Listing
March 2021

Comparison of Nasopharyngeal MR, F-FDG PET/CT, and F-FDG PET/MR for Local Detection of Natural Killer/T-Cell Lymphoma, Nasal Type.

Front Oncol 2020 14;10:576409. Epub 2020 Oct 14.

Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Objectives: The present study aims to compare the diagnostic efficacy of MR, F-FDG PET/CT, and F-FDG PET/MR for the local detection of early-stage extranodal natural killer/T-cell lymphoma, nasal type (ENKTL).

Patients And Methods: Thirty-six patients with histologically proven early-stage ENKTL were enrolled from a phase 2 study (Cohort A). Eight nasopharyngeal anatomical regions from each patient were imaged using F-FDG PET/CT and MR. A further nine patients were prospectively enrolled from a multicenter, phase 3 study; these patients underwent F-FDG PET/CT and PET/MR after a single F-FDG injection (Cohort B). Region-based sensitivity and specificity were calculated. The standardized uptake values (SUV) obtained from PET/CT and PET/MR were compared, and the relationship between the SUV and apparent diffusion coefficients (ADC) of PET/MR were analyzed.

Results: In Cohort A, of the 288 anatomic regions, 86 demonstrated lymphoma involvement. All lesions were detected by F-FDG PET/CT, while only 70 were detected by MR. F-FDG PET/CT exhibited a higher sensitivity than MR (100% vs. 81.4%, χ = 17.641, < 0.001) for local detection of malignancies. The specificity of F-FDG PET/CT and MR were 98.5 and 97.5%, respectively (χ = 0.510, = 0.475). The accuracy of F-FDG PET/CT was 99.0% and the accuracy of MR was 92.7% (χ = 14.087, < 0.001). In Cohort B, 72 anatomical regions were analyzed. PET/CT and PET/MR have a sensitivity of 100% and a specificity of 92.5%. The two methods were consistent (κ = 0.833, < 0.001). There was a significant correlation between PET/MR SUVmax and PET/CT SUVmax ( = 0.711, < 0.001), and SUVmean ( = 0.685, < 0.001). No correlation was observed between the SUV and the ADC.

Conclusion: In early-stage ENKTL, nasopharyngeal MR showed a lower sensitivity and a similar specificity when compared with F-FDG PET/CT. PET/MR showed similar performance compared with PET/CT.
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http://dx.doi.org/10.3389/fonc.2020.576409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591820PMC
October 2020

Development of a dual-energy spectral CT based nomogram for the preoperative discrimination of mutated and wild-type KRAS in patients with colorectal cancer.

Clin Imaging 2021 Jan 6;69:205-212. Epub 2020 Sep 6.

Key Laboratory of Medical Imaging, Gansu Province, Lanzhou, China; Department of Radiology, Lanzhou University Second Hospital, Lanzhou, China. Electronic address:

Purpose: To develop a dual-energy spectral CT (DESCT) nomogram for the preoperative identification of KRAS mutation in patients with colorectal cancer (CRC).

Method: One hundred and twenty-four patients who underwent energy spectrum CT pre-operatively were recruited and split into mutated KRAS group (n = 50) and wild-type KRAS group (n = 74). DESCT parameters, including monochromatic CT value, iodine concentration, water concentration, and effective atomic number were measured independently by two reviewers in the arterial, venous, and delayed phases. Normalized iodine concentration (NIC) and slope k of the spectral HU curve were calculated. Evaluate other imaging features such as ATL/LTL ratio, tumor gross pattern, pericolorectal fat invasion (PFI) was also performed by these reviewers. Independent predictors for KRAS mutation were screened out using logistic regression, and these predictors were presented as a nomogram. The receiver operating characteristic (ROC) curve, calibration curve and decision curve analysis (DCA) were used to evaluate the clinical usefulness of the nomogram.

Results: The slope k in the arterial phase, effective atomic number in the arterial phase, NIC in the venous phase, ATL/LTL ratio and PFI were significant independent predictors for KRAS mutation. Based on these independent predictors, a quantitative nomogram was developed to predict individual KRAS mutation probability. The nomogram had excellent performance with an AUC of 0.848 and excellent calibration. DCA showed that our nomogram has outstanding clinical utility.

Conclusions: This study demonstrates that a DESCT based nomogram has potential value for individual preoperative identification of KRAS mutation in CRC patients.
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http://dx.doi.org/10.1016/j.clinimag.2020.08.023DOI Listing
January 2021

Efficacy Evaluation of Early, Low-Dose, Short-Term Corticosteroids in Adults Hospitalized with Non-Severe COVID-19 Pneumonia: A Retrospective Cohort Study.

Infect Dis Ther 2020 Dec 2;9(4):823-836. Epub 2020 Sep 2.

Department of Liver Disease, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Objectives: This study aimed to observe the efficacy of corticosteroids in non-severe COVID-19 pneumonia.

Methods: A retrospective study based on propensity score matching was designed to explore the effects of corticosteroids. Primary outcomes included the rate of patients who developed severe disease and mortality. Secondary outcomes included duration of fever, virus clearance time, length of hospital stay, and the use of antibiotics.

Results: A total of 475 patients with non-severe COVID-19 pneumonia were enrolled, 55 patients received early, low-dose, and short-term corticosteroids therapy, 420 patients received non-corticosteroids therapy. Compared to the non-corticosteroids group, there was a prolonged duration of fever (median 5 vs 3 days, p < 0.001), virus clearance time (median 18 vs 11 days, p < 0.001), and length of hospital stay (median 23 vs 15 days, p < 0.001) in the corticosteroids group. The percentages of antibiotics therapy (89.1% vs 23.6%, p < 0.001), use of at least two antibiotics (38.2% vs 12.7%, p = 0.002), and antifungal therapy (7.3% vs 0, p = 0.042) were higher in the corticosteroids group than those in the non-corticosteroids group. Compared to the non-corticosteroids group, more patients developed severe disease (12.7% vs 1.8%, p = 0.028) in the corticosteroids group. There was no significant difference between the two groups in mortality (1.8% vs 0, p = 0.315).

Conclusion: In adult patients with non-severe COVID-19 pneumonia, early, low-dose, and short-term corticosteroids therapy was associated with worse clinical outcomes.
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http://dx.doi.org/10.1007/s40121-020-00332-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467137PMC
December 2020

Intrahepatic manifestation and distant extrahepatic disease in alveolar echinococcosis: a multicenter cohort study.

Acta Radiol 2020 Aug 26:284185120951958. Epub 2020 Aug 26.

Department of Internal Medicine I, University Hospital Ulm, Ulm, Germany.

Background: The main endemic areas of alveolar echinococcosis (AE) are in central Europe and western China. The infiltration of intrahepatic vascular and bile ducts as well as extrahepatic disease can lead to complications and may increase morbidity in AE.

Purpose: To evaluate the vascular/biliary involvement of hepatic alveolar echinococcosis (HAE) and distant extrahepatic disease at each of four locations in Germany, France, and China.

Material And Methods: Contrast-enhanced abdominal magnetic resonance imaging (MRI) scans of patients with HAE, 200 in total, were evaluated by five examiners. AE liver lesions were classified according to Kodama's classification. Furthermore, distant extrahepatic manifestations were documented with additionally performed imaging modalities. Vascular/biliary involvement of hepatic manifestations as well as the presence of extrahepatic manifestations were correlated with the respective Kodama type of the liver lesion.

Results: Distant extrahepatic AE manifestations were significantly more frequent in China than in Europe (12/100 vs. 3/100; Fisher's exact test: =0.0286). A significant relationship exists between presence of distant extrahepatic disease manifestation and size of the AE liver lesion (132.53 ± 48.65 vs. 92.49 ± 50.06;  = 0.0030). Vascular/biliary involvement is significantly more frequent in China than in Europe (86/100 vs. 65/100; χ = 11.92;  = 0.0006). Vascular/biliary involvement depends on lesion size (111.10 ± 47.44 vs. 47.36 ± 24.36; <0.0001). Different types of AE liver lesions are associated with differences in vascular/biliary involvement and extrahepatic manifestations.

Conclusion: Vascular/biliary involvement and presence of distant extrahepatic manifestations depend on size of the HAE lesions and are more frequently detected in China. Different MRI morphological patterns influence vascular/biliary involvement and the occurrence of distant extrahepatic manifestations.
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http://dx.doi.org/10.1177/0284185120951958DOI Listing
August 2020

A simple algorithm helps early identification of SARS-CoV-2 infection patients with severe progression tendency.

Infection 2020 Aug 21;48(4):577-584. Epub 2020 May 21.

Department of Liver Disease, Shanghai Public Health Clinical Center, Fudan University, 2901 Cao Lang Road, Shanghai, 201508, China.

Objectives: We aimed to develop a simple algorithm to help early identification of SARS-CoV-2 infection patients with severe progression tendency.

Methods: The univariable and multivariable analysis were computed to identify the independent predictors of COVID-19 progression. The prediction model was established in a retrospective training set of 322 COVID-19 patients and was re-evaluated in a prospective validation set of 317 COVID-19 patients.

Results: The multivariable analysis identified age (OR = 1.061, p = 0.028), lactate dehydrogenase (LDH) (OR = 1.006, p = 0.037), and CD4 count (OR = 0.993, p = 0.006) as the independent predictors of COVID-19 progression. Consequently, the age-LDH-CD4 algorithm was derived as (age × LDH)/CD4 count. In the training set, the area under the ROC curve (AUROC) of age-LDH-CD4 model was significantly higher than that of single CD4 count, LDH, or age (0.92, 0.85, 0.80, and 0.75, respectively). In the prospective validation set, the AUROC of age-LDH-CD4 model was also significantly higher than that of single CD4 count, LDH, or age (0.92, 0.75, 0.81, and 0.82, respectively). The age-LDH-CD4 ≥ 82 has high sensitive (81%) and specific (93%) for the early identification of COVID-19 patients with severe progression tendency.

Conclusions: The age-LDH-CD4 model is a simple algorithm for early identifying patients with severe progression tendency following SARS-CoV-2 infection, and warrants further validation.
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http://dx.doi.org/10.1007/s15010-020-01446-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240242PMC
August 2020

Hepatic alveolar echinococcosis: Comparative computed tomography study between two Chinese and two European centres.

Food Waterborne Parasitol 2020 Jun 11;19:e00082. Epub 2020 May 11.

Xinjiang Medical University, First Affiliated Hospital, WHO Collaborating Centre on Prevention and Care Management of Echinococcosis, 830054 Urumqi, Xinjiang Uyghur Autonomous Region, PR China.

The main endemic areas for alveolar echinococcosis (AE) are in Central Europe and Western China, and in >98% of cases, AE manifests in the liver. The aim of this work was to compare European and Chinese patient groups for number, size, and computed tomography (CT) appearance of hepatic AE lesions. A total of 200 CT scans of patients with hepatic AE were evaluated by four blinded, experienced radiologists from two European (Besancon, Ulm) and two Chinese centres (Xining, Urumqi). In addition to noting the number, size, and localisation of the lesions, the radiologists evaluated morphological appearance using the Ulm Classification - CT scheme. Chinese patients were younger than European patients (36.8 ± 13.2 vs. 63.5 ± 17.7;  < 0.0001) and had significantly larger lesions (120.4 ± 50.8 vs. 70.9 ± 39.8; p < 0.0001). The morphological appearance of the lesions on CT differed significantly between the two groups ( < 0.05), as did the number of lesions (2.6 ± 3.9 in European centres versus 3.8 ± 5.0 in Chinese centres;  = 0.0062). Patient age and AE-related morphological manifestations differ between Europe and China, but the reasons for the differences are unknown.
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http://dx.doi.org/10.1016/j.fawpar.2020.e00082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232088PMC
June 2020

Anesthesia Management and Perioperative Infection Control in Patients With the Novel Coronavirus.

J Cardiothorac Vasc Anesth 2021 05 29;35(5):1503-1508. Epub 2020 Mar 29.

University of Colorado, Aurora, CO.

Anesthesiologists have a high risk of infection with COVID-19 during perioperative care and as first responders to airway emergencies. The potential of becoming infected can be reduced by a systematic and integrated approach that assesses infection risk. The latter leads to an acceptable choice of materials and techniques for personal protection and prevention of cross-contamination to other patients and staff. The authors have presented a protocolized approach that uses diagnostic criteria to clearly define benchmarks from the medical history along with clinical symptoms and laboratory tests. Patients can then be rapidly assigned into 1 of 3 risk categories that direct the choice of protective materials and/or techniques. Each hospital can adapt this approach to develop a system that fits its individual resources. Educating medical staff about the proper use of high-risk areas for containment serves to protect staff and patients.
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http://dx.doi.org/10.1053/j.jvca.2020.03.035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146651PMC
May 2021

Worldwide literature on epidemiology of human alveolar echinococcosis: a systematic review of research published in the twenty-first century.

Infection 2019 Oct 30;47(5):703-727. Epub 2019 May 30.

Qinghai University Affiliated Hospital, Qinghai University, Xining, 810001, Qinghai, People's Republic of China.

Purpose: Human alveolar echinococcosis (AE) is a potentially lethal zoonosis caused by the cestode Echinococcus multilocularis. The aim of this systematic review is to establish a comprehensive global AE literature overview taking into account the epidemiologically relevant AE research of the twenty-first century.

Methods: We systematically searched the global literature published from 2001 through 2018 via MEDLINE, EMBASE, the Russian databases eLIBRARY.RU, CyberLeninka, the Chinese databases CNKI, VIP, Journals.

Research: ac.ir (Farsi language-based), Jordan E-Library (Arab language-based) and supplementary Google Scholar, in accordance with the PRISMA guidelines. QGIS software was used for the mapping of the affected countries.

Results: We have listed 154 relevant publications in the final literature synopsis in consideration of our quality assessment. Including non-autochthonous cases, human AE was reported in 36 countries within the northern hemisphere from 2001 to 2018. The first publication of AE in Tajikistan, Pakistan, South Korea, Belgium, the Netherlands, Slovakia, Hungary, Lithuania, Latvia, Slovenia and Morocco occurred in this century; further first cases in Taiwan, Thailand, and Denmark were considered to be non-autochthonous by the authors. The highest total case numbers (n ≥ 100 in a single article) were reported in France, Germany, Switzerland, Poland, and Lithuania, including China and Kyrgyzstan with by far the highest prevalence figures.

Conclusions: Our paper emphasises the increasing spread of reported cases and the rise in its numbers in the literature of the twenty-first century, especially in western, northern and eastern Europe, as well as in central Asia. Epidemiological studies on human infections are lacking in many parts of the world.
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http://dx.doi.org/10.1007/s15010-019-01325-2DOI Listing
October 2019

Magnetic resonance imaging evaluation of characteristics of vascular invasion in intermediate and advanced hepatic alveolar echinococcosis.

Exp Ther Med 2019 May 1;17(5):4197-4204. Epub 2019 Apr 1.

Imaging Center of Affiliated Hospital of Qinghai University, Xining, Qinghai 810001, P.R. China.

The objective of the present study was to provide a basis for the personalized treatment of intermediate and advanced hepatic alveolar echinococcosis (HAE) by elucidating the characteristics of vascular invasion and lesion growth. A total of 160 patients with intermediate and advanced HAE who were subjected to plain as well as contrast-enhanced 3.0-T magnetic resonance imaging prior to surgery were analyzed. Pathological and intra-operative observations of the subjects were also considered. The size and location of HAE lesions, vascular invasion characteristics and growth patterns were assessed. A total of 78 patients (48.75%) had lesions involving the S5-8 segment/partial right liver lobe, 21 (13.13%) had involvement in the S2-4 segment/partial left liver lobe and 61 (38.13%) had lesions that transcended the left and right liver lobes. Pathological examination revealed that the vascular invasion rates of the hepatic portal veins, intrahepatic veins (left, central and right vein, and inferior vena cava) and hepatic arteries were 51.88, 43.28 and 26.87%, respectively. Liver hilum invasion was observed in 128 patients (80.00%), 71 of which (44.38%) presented with invasion of the primary porta hepatis, 11 (6.88%) with invasion of the secondary porta hepatis and 46 (28.75%) with invasion of the primary as well as the secondary porta hepatis. In conclusion, the growth pattern of intermediate and advanced HAE is determined by the site, blood supply and activity of the lesion. The current study demonstrated that lesions tend to invade the intrahepatic venous system and porta hepatis, and to target veins rather than arteries.
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http://dx.doi.org/10.3892/etm.2019.7460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468930PMC
May 2019

A nanocomposite consisting of an amorphous seed and a molecularly imprinted covalent organic framework shell for extraction and HPLC determination of nonsteroidal anti-inflammatory drugs.

Mikrochim Acta 2019 01 9;186(2):76. Epub 2019 Jan 9.

Department of Chemistry, Zhejiang University, Xixi Campus, Hangzhou, 310028, China.

A novel nanocomposite consisting of an amorphous seed and a molecularly imprinted covalent organic framework shell was prepared via a heterogeneous nucleation and growth method. By using ibuprofen as the dummy template, a molecularly imprinted covalent organic framework (MICOF) with a large surface area was prepared from 1,3,5-triformylbenzene and 4,4'-diaminobiphenyl. It was placed on the surface of monodisperse amorphous seeds. Owing to strong π-interaction, the [email protected] nanocomposite displays fast binding kinetics, large adsorption capacities and selectivity for nonsteroidal anti-inflammatory drugs (NSAIDs). Following desorption from the [email protected] with methanol containing 1% ammonium hydroxide, the NSAIDs ketoprofen, ibuprofen, diclofenac, indomethacin, flurbiprofen and naproxen were quantified by HPLC with UV detection. Under optimized conditions, the method exhibits good linearity within the range of 0.002-1.0 μg mL-1, low limits of detection (0.38-2.92 μg L-1), and acceptable repeatability. The recoveries of NSAIDs at three spiking levels range from 77 to 112%, and the RSDs are <9.4%. The method was successfully applied to the analysis of NSAIDs in spiked environmental water samples. Graphical abstract A molecular imprinted covalent organic framework nanocomposite ([email protected]) was prepared by heterogeneous nucleation and growth method. It was explored as a sorbent for the solid phase extraction of nonsteroidal anti-inflammatory drugs before determination by HPLC with UV detection.
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http://dx.doi.org/10.1007/s00604-018-3187-6DOI Listing
January 2019

The associations of long-time mobile phone use with sleep disturbances and mental distress in technical college students: a prospective cohort study.

Sleep 2019 02;42(2)

Department of Psychiatry, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Study Objectives: To determine the longitudinal associations of long-time mobile phone use (LTMPU) with sleep disturbances and mental distress in a prospective cohort of technical college students.

Methods: A total of 4333 (response rate: 91.5%) and 3396 (response rate: 78.4%) participants were recruited at baseline and 8-month follow-up, respectively. Data were collected by a set of questionnaires including socio-demographics, lifestyle practice, duration of mobile phone use per day, sleep patterns on weekdays and weekends, as well as Insomnia Severity Index, Epworth Sleepiness Scale, reduced Morningness-Eveningness Questionnaire, Beck Depression Inventory, and Zung Self-Rating Anxiety Scale. LTMPU was defined as using mobile phone ≥4 hours/day.

Results: At baseline, 23.5% (n = 1020) of the participants reported using mobile phone ≥ 4 hours/day. LTMPU at baseline was positively associated with the new incidences (range, adjusted odds ratio 1.31-1.53) of a series of the sleep disturbances and mental distress at follow-up. The discontinuation of LTMPU was associated with an amelioration of the risks of most of these problems. Cross-lagged analyses revealed bidirectional associations of the duration of mobile phone use with poor sleep and mental health outcomes.

Conclusions: LTMPU predicts the new incidences of most sleep disturbances and mental distress, while discontinuation of LTMPU is associated with amelioration of these problems. Moreover, there are bidirectional associations between the duration of mobile phone use and various sleep and mental outcomes. These findings highlight the critical role of prevention and early recognition of excessive mobile phone use and their accompanied mental and sleep problems.
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http://dx.doi.org/10.1093/sleep/zsy213DOI Listing
February 2019

A novel diagnostic algorithm to predict significant liver inflammation in chronic hepatitis B virus infection patients with detectable HBV DNA and persistently normal alanine transaminase.

Sci Rep 2018 10 18;8(1):15449. Epub 2018 Oct 18.

Department of Liver Disease, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.

Significant liver inflammation might be found in 20-34% of chronic hepatitis B virus (HBV) infection patients with detectable HBV DNA and persistently normal alanine transaminase (ALT) (PNALT). We aimed to develop a diagnostic algorithm to predict significant liver inflammation in these specific patients. Using liver biopsy as the gold standard, we developed a novel, simple diagnostic algorithm to predict significant liver inflammation in a training set of 365 chronic HBV infection patients with detectable HBV DNA and PNALT, and validated the diagnostic accuracy in a validation set of 164 similar patients. The novel algorithm (AAGP) attributed to age, ALT, gamma-glutamyl transpeptidase (GGT), and platelet count was developed. In the training set, the area under the receiver operating characteristic curve (AUROC) of AAGP was higher than that of ALT and aspartate transaminase (AST), to diagnose significant liver inflammation (0.77, 0.67, and 0.59, respectively, p < 0.001). In the validation set, the AUROC of AAGP was also higher than ALT and AST (0.75, 0.61, and 0.54, respectively, p < 0.001). Using AAGP ≥2, the sensitivity and negative predictive value (NPV) was 91% and 93%, respectively, to diagnose significant liver inflammation. Using AAGP ≥8, the specificity and NPV was 91% and 86%, respectively, for significant liver inflammation. In conclusion, the AAGP algorithm is a novel, simple, user-friendly algorithm for the diagnosis of significant liver inflammation in chronic HBV infection patients with detectable HBV DNA and PNALT.
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http://dx.doi.org/10.1038/s41598-018-33412-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193950PMC
October 2018

Post-marketing safety surveillance and re-evaluation of Xueshuantong injection.

BMC Complement Altern Med 2018 Oct 16;18(1):277. Epub 2018 Oct 16.

The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, 450000, People's Republic of China.

Background: Traditional Chinese medicine injections (TCMIs) have been widely used to treat severe and acute diseases due to their high bioavailability, accurate curative effect, and rapid effect. However, incidence rates of adverse drug reactions (ADRs) of TCMIs have also increased in recent years. Xueshuantong injection (XSTI) is a commonly-used TCMI comprised of Panax notoginseng total sapiens for the treatment of stroke hemiplegia, chest pain, and central retinal vein occlusion. Its safety remains uncelar. Therefore, post-marketing safety of XSTI was studied in this research.

Methods: In present study, post-marketing safety surveillance and re-evaluation of XSTI were reported. Thirty thousand eight hundred eighty-four patients in 33 hospitals from 7 provinces participated in this study. Incidence rate, most common clinical manifestations, types, severity, occurrence time, and disposal of ADRs were calculated.

Results: Incidence rate of ADR of XSTI was 4.14‰ and the most common clinical manifestations were skin and its appendages damage. Type A accounts for 95.49% of ADRs of XSTI and most of them (41.41%) were occurred within 24 h after receiving XSTI treatment. Severities of most ADRs of XSTI were moderate reactions (86.72%). Main disposition of ADRs of XSTI was drug withdrawal and symptomatic treatment (54.69%).

Conclusions: Our data provide basis for improvement of instructions of XSTI and clinical safety of XSTI. Post-marketing surveillance of TCMIs in this study is a powerful tool to identify types and manifestations of ADRs to improve safety and effectiveness of drugs in clinical applications.

Trial Registration: This protocol has international registration in China clinical trial registration center ( ChiCTR~OPC~ 14,005,718 ) at December 22, 2014.
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http://dx.doi.org/10.1186/s12906-018-2329-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192149PMC
October 2018

Extracellular Hepatitis B Virus RNAs Are Heterogeneous in Length and Circulate as Capsid-Antibody Complexes in Addition to Virions in Chronic Hepatitis B Patients.

J Virol 2018 12 27;92(24). Epub 2018 Nov 27.

Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai, China

Extracellular HBV RNA has been detected in both HBV-replicating cell culture media and sera from chronic hepatitis B (CHB) patients, but its exact origin and composition remain controversial. Here, we demonstrated that extracellular HBV RNA species were of heterogeneous lengths, ranging from the length of pregenomic RNA to a few hundred nucleotides. In cell models, these RNAs were predominantly associated with naked capsids, although virions also harbored a minority of them. Moreover, HBV RNAs in hepatitis B patients' blood circulation were localized in unenveloped capsids in the form of capsid-antibody complexes (CACs) and in virions. Furthermore, we showed that extracellular HBV RNAs could serve as the template for viral DNA synthesis. In conclusion, extracellular HBV RNAs mainly consist of pgRNA or the pgRNA species degraded by the RNase H domain of the polymerase in the process of viral DNA synthesis and circulate as CACs and virions. Their presence in blood circulation of CHB patients may be exploited to develop novel biomarkers for HBV persistence. Although increasing evidence suggests the presence of extracellular HBV RNA species, their origin and molecular forms are still under debate. In addition to the infectious virions, HBV is known to secrete several species of incomplete viral particles, including hepatitis B surface antigen (HBsAg) particles, naked capsids, and empty virions, during its replication cycle. Here, we demonstrated that extracellular HBV RNAs were associated with naked capsids and virions in HepAD38 cells. Interestingly, we found that unenveloped capsids circulate in the blood of hepatitis B patients in the form of CACs and, together with virions, serve as vehicles carrying these RNA molecules. Moreover, extracellular HBV RNAs are heterogeneous in length and represent either pregenomic RNA (pgRNA) or products of incomplete reverse transcription during viral replication. These findings provide a conceptual basis for further application of extracellular RNA species as novel biomarkers for HBV persistence.
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http://dx.doi.org/10.1128/JVI.00798-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258948PMC
December 2018

Determination of nitenpyram and 6-chloronicotinic acid in environmental samples by ion chromatography coupled with online photochemically induced fluorescence detector.

J Sep Sci 2018 Nov 9;41(22):4096-4104. Epub 2018 Oct 9.

Department of chemistry, Xixi Campus, Zhejiang University, Hangzhou, P. R. China.

A simple, cost-effective, sensitive, and quick method for the determination of nitenpyram and its metabolite 6-chloronicotinic acid in environmental samples was developed by coupling an ion chromatograph with a fluorescence detector and a post-column photochemical reactor. This developed analytical method involved a rapid sample extraction by modified and miniaturized quick, easy, cheap, effective, rugged, and safe method followed by isocratic ion chromatographic separation of nitenpyram and 6-chloronicotinic acid into an IonPac AS11-HC column protected by IonPac AG11A guard column by running 30 mM NaOH + 10% acetonitrile mobile phase. A homemade post-column photochemical reactor was also integrated with the ion chromatographic system for online transformation of both analytes into their respective highly fluorescent photoproduct in basic media without using an extra pump. The developed method was validated by following SANTE/11945/2015 guidelines on analytical quality control and validation procedures. The method showed a good linear response (r > 0.999), improved limit of detection (0.101-0.132 μg/L), minimum or no matrix effect, excellent recoveries (90.2-100.10%) and relative standard deviations were found to be ≤6.50%.
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http://dx.doi.org/10.1002/jssc.201800612DOI Listing
November 2018

Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization.

R Soc Open Sci 2018 Aug 15;5(8):171948. Epub 2018 Aug 15.

Department of Chemistry, Zhejiang University, Xixi Campus, Hangzhou 310028, People's Republic of China.

A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM HPO and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0 mg l with a correlation coefficient of 0.9997. The limit of qualification (/ = 10) and limit of detection (/ = 3) were 9.7 and 2.9 µg l, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.
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http://dx.doi.org/10.1098/rsos.171948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124075PMC
August 2018

Self-assembling covalent organic framework functionalized poly (styrene-divinyl benzene-glycidylmethacrylate) composite for the rapid extraction of non-steroidal anti-inflammatory drugs in wastewater.

J Chromatogr A 2018 Oct 9;1571:76-83. Epub 2018 Aug 9.

Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China. Electronic address:

The growing use of non-steroidal anti-inflammatory drugs (NSAIDs) has seriously affected human health and ecosystems, as a result, the World Health Organization (WHO) has regarded them as emerging contaminants. NSAID's polar nature and trace amount present in wastewater make their extraction and determination challenging in modern analytical science. Therefore, regarding the need, we herein report a rapid, sensitive and selective in-syringe solid-phase extraction (SPE) method coupled with ultrahigh performance liquid chromatography and UV detector for the determination of seven NSAIDs in environmental water samples. Specifically, the NSAIDs in water samples were directly extracted by using synthesized porous covalent organic framework functionalized poly (styrene-divinyl benzene-glycidylmethacrylate) composite ([email protected]) without further cleanup procedures. The adsorption of NSAIDs on [email protected] sorbent was investigated by using adsorption isotherms and kinetic studies. Various parameters, including amount of sorbent, pH of the samples, the volume of desorption solvent, and desorption time that were involved in in-syringe SPE were optimized. Under the optimized conditions, good linearity was observed at 0.005-5.0 μg mL with method quantification limits (LOQs) estimated between 0.54-2.74 μg L. The recoveries of the seven NSAIDs at the level of 0.5, 5.0 and 20.0 μg L were ranging from 84.3% to 99.6%.
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http://dx.doi.org/10.1016/j.chroma.2018.08.019DOI Listing
October 2018

Upregulation of miR-191 promotes cell growth and invasion via targeting TIMP3 in prostate cancer.

J BUON 2018 Mar-Apr;23(2):444-452

Department of Laboratory Medicine, Medical Technology Affiliated Hospital, College of Medicine, Hebei University of Engineering, Handan 056002, P.R.China.

Purpose: Prostate cancer (PCa) is the most frequently malignant neoplasm in men. MicroRNAs (miRs) have been identified to play important biological roles in a variety of tumors. Several studies showed that miR-191 was involved in the development of different cancers, but its role in prostate cancer remains unclear.

Methods: Human PCa cell lines DU145, PC-3 and LNCAP, and benign prostate hyperplasia (BPH) and human prostate epithelial cell line RWPE-1 were used. The expression level of miR-191 in 48 paired prostate tumor and adjacent normal tissues was assessed along with the clinical patient features. Synthetic miR-191 mimics and inhibitors were used to overexpress or inhibit the miR-191 level. CCK8 and colony formation assay were used to evaluate the cell growth. The ability of cell invasion was studied by transwell assay. Dual-luciferase experiment was used to identify the target gene and western blot was performed to evaluate the protein level.

Results: miR-191 was overexpressed in PCa tissue samples compared to the normal group as well in PCa-derived cell lines. Upregulation miR-191 in PC-3 cells significantly promoted while downregulation miR-191 in DU145 cells retarded the cell proliferation and invasion. Furthermore, TIMP3 were proved to be a direct target gene of miR-191 and knockdown of TIMP3 reversed the function of miR-191 downregulation.

Conclusion: This study demonstrated that miR-191 promoted the cell growth and invasion ability in prostate cancer through downregulating TIMP3 and might be a potential target for the biotherapy for PCa.
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August 2019

Hydrothermal Synthesized of CoMoO Microspheres as Excellent Electrode Material for Supercapacitor.

Nanoscale Res Lett 2018 Apr 24;13(1):120. Epub 2018 Apr 24.

Laboratory of Functional Materials and Henan Key Laboratory of Photovoltaic Materials, College of Physics and Materials Science, Henan Normal University, No. 46 Construction East Road, Xinxiang, 453007, Henan, China.

The single-phase CoMoO was prepared via a facile hydrothermal method coupled with calcination treatment at 400 °C. The structures, morphologies, and electrochemical properties of samples with different hydrothermal reaction times were investigated. The microsphere structure, which consisted of nanoflakes, was observed in samples. The specific capacitances at 1 A g are 151, 182, 243, 384, and 186 F g for samples with the hydrothermal times of 1, 4, 8, 12, and 24 h, respectively. In addition, the sample with the hydrothermal time of 12 h shows a good rate capability, and there is 45% retention of initial capacitance when the current density increases from 1 to 8 A g. The high retain capacitances of samples show the fine long-cycle stability after 1000 charge-discharge cycles at current density of 8 A g. The results indicate that CoMoO samples could be a choice of excellent electrode materials for supercapacitor.
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http://dx.doi.org/10.1186/s11671-018-2540-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915980PMC
April 2018

Serum hepatitis B surface antigen levels predict insignificant fibrosis and non-cirrhosis in hepatitis B e antigen positive patients with normal or mildly elevated alanine transaminase levels.

Oncotarget 2017 Oct 23;8(49):86463-86470. Epub 2017 Sep 23.

Department of Hepatitis, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.

Background/aims: We aimed to evaluate the diagnostic value of serum hepatitis B surface antigen (HBsAg) levels for liver fibrosis in hepatitis B e antigen-positive [HBeAg (+)] chronic hepatitis B (CHB) patients with alanine transaminase (ALT)≤twice upper limit of normal (ULN).

Methods: 505 patients who underwent liver biopsies and HBsAg quantitative detections were included. Liver histology was scored using METAVIR scoring system. The area under the receiver-operator curve (AUROC) was used to determine the diagnostic accuracy.

Results: Of 505 CHB patients, 333 have HBeAg (+), and 172 have HBeAg (-). HBsAg levels and METAVIR fibrosis scores showed strong correlation (r=-0.50, <0.001) in HBeAg (+) patients, but no correlation in HBeAg (-) patients (r=0.09, =0.239). HBeAg (+) patients with insignificant fibrosis (F0-1) exhibited higher HBsAg levels than those with significant fibrosis (F2-4) (4.60 4.12 log10IU/ml, <0.001). HBeAg (+) patients with non-cirrhosis (F0-3) exhibited higher HBsAg levels than those with cirrhosis (F4) (4.48 3.95 log10IU/ml, <0.001). In this study, the AUROC of HBsAg was 0.86 for diagnosing insignificant fibrosis, and 0.91 for diagnosing non-cirrhosis in HBeAg (+) CHB patients.

Conclusions: Serum HBsAg level can identify insignificant fibrosis and non-cirrhosis in HBeAg (+) CHB patients with ALT≤2 ULN, and thus avoid liver biopsy in this population.
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http://dx.doi.org/10.18632/oncotarget.21210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689698PMC
October 2017
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