Publications by authors named "Wei-Wei Wu"

86 Publications

A specially designed medical screen for children suffering from burns: A randomized trial of a distraction-type therapy.

Burns 2020 May 30. Epub 2020 May 30.

Department of Burn Surgery, The First Hospital of Jilin University, Chaoyang District, Xinmin Street, Street 71, Changchun City, Jilin Province 130021, China. Electronic address:

Objective: To evaluate the impact of the specially designed medical dressing screen during wound dressing changes of children who suffered burns to their hand or foot.

Design: Randomized controlled trial.

Setting: Burns and Plastic Reconstruction Unit.

Participants: Children (N=120) with burns on up to 1-5% of the total body surface area.

Interventions: The patients were selected and randomly allocated to 3 equal-sized groups as follows: control group (N=40): the children received only regular dressing changes; computer group (N=40): a touch-screen computer was used for children during dressing changes; medical screen group (N=40): a medical screen combined with the touch-screen computer were used for children during dressing changes. All patients underwent a dressing change once per day for four days. Data were distributed four times: immediately after the initial dressing change (T1); and immediately after each times at next three consecutive days (T2-T4).

Main Outcome Measures: The Pain level of the children evaluated by medical staffs was the primary outcome, the Pain level of the children evaluated by children's parents and the satisfaction of wound therapist were used as second outcomes.

Results: The mean scores related to pain level at the medical screen group displayed significantly better results than those of control group and those of the computer group. Additionally, the results of the pain evaluated by parents and satisfaction score of the wound therapist at the medical screen group was also better than other groups.

Conclusions: This study demonstrated "that the" application of the medical screen for burns can relieve the pain of 1-3 years old children suffering from a burns during dressing changes. Additionally, the application of the medical screen also increased the satisfaction of the parents and the wound therapist performing the dressing changes.
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http://dx.doi.org/10.1016/j.burns.2020.05.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261105PMC
May 2020

Alteration of twinfilin1 expression underlies opioid withdrawal-induced remodeling of actin cytoskeleton at synapses and formation of aversive memory.

Mol Psychiatry 2021 May 7. Epub 2021 May 7.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Exposure to drugs of abuse induces alterations of dendritic spine morphology and density that has been proposed to be a cellular basis of long-lasting addictive memory and heavily depend on remodeling of its underlying actin cytoskeleton by the actin cytoskeleton regulators. However, the actin cytoskeleton regulators involved and the specific mechanisms whereby drugs of abuse alter their expression or function are largely unknown. Twinfilin (Twf1) is a highly conserved actin-depolymerizing factor that regulates actin dynamics in organisms from yeast to mammals. Despite abundant expression of Twf1 in mammalian brain, little is known about its importance for brain functions such as experience-dependent synaptic and behavioral plasticity. Here we show that conditioned morphine withdrawal (CMW)-induced synaptic structure and behavior plasticity depends on downregulation of Twf1 in the amygdala of rats. Genetically manipulating Twf1 expression in the amygdala bidirectionally regulates CMW-induced changes in actin polymerization, spine density and behavior. We further demonstrate that downregulation of Twf1 is due to upregulation of miR101a expression via a previously unrecognized mechanism involving CMW-induced increases in miR101a nuclear processing via phosphorylation of MeCP at Ser421. Our findings establish the importance of Twf1 in regulating opioid-induced synaptic and behavioral plasticity and demonstrate its value as a potential therapeutic target for the treatment of opioid addiction.
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http://dx.doi.org/10.1038/s41380-021-01111-3DOI Listing
May 2021

Editorial: Low-Dimension Sensing Nanomaterials.

Front Chem 2021 12;9:608327. Epub 2021 Apr 12.

Department of Chemistry, College of Science, Shanghai University, Shanghai, China.

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http://dx.doi.org/10.3389/fchem.2021.608327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072664PMC
April 2021

Weighted Gene Coexpression Network Analysis Reveals Essential Genes and Pathways in Bipolar Disorder.

Front Psychiatry 2021 17;12:553305. Epub 2021 Mar 17.

Peking University Sixth Hospital, Peking University Institute of Mental Health, Peking University, Beijing, China.

Bipolar disorder (BD) is a major and highly heritable mental illness with severe psychosocial impairment, but its etiology and pathogenesis remains unclear. This study aimed to identify the essential pathways and genes involved in BD using weighted gene coexpression network analysis (WGCNA), a bioinformatic method studying the relationships between genes and phenotypes. Using two available BD gene expression datasets (GSE5388, GSE5389), we constructed a gene coexpression network and identified modules related to BD. The analyses of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways were performed to explore functional enrichment of the candidate modules. A protein-protein interaction (PPI) network was further constructed to identify the potential hub genes. Ten coexpression modules were identified from the top 5,000 genes in 77 samples and three modules were significantly associated with BD, which were involved in several biological processes (e.g., the actin filament-based process) and pathways (e.g., MAPK signaling). Four genes (, and ) were identified as candidate hub genes by PPI analysis and CytoHubba. Finally, we carried out validation analyses in a separate dataset, GSE12649, and verified as a hub gene and the involvement of several biological processes such as actin filament-based process and axon development. Taken together, our findings revealed several candidate pathways and genes () in the pathogenesis of BD and call for further investigation for their potential research values in BD diagnosis and treatment.
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http://dx.doi.org/10.3389/fpsyt.2021.553305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010671PMC
March 2021

Outcomes for Pauwels' Osteotomy in Nonunions of Displaced Femoral Neck Stress Fractures: A Case Series.

J Surg Orthop Adv 2020 ;29(4):234-239

Department of Orthopaedic Surgery, Naval Medical Center San Diego, San Diego, California.

Femoral neck stress fractures (FNSF) are rare injuries and have shown poor results after displacement, including nonunion and osteonecrosis (ON). The goal of this study was to retrospectively evaluate a series of patients who underwent a valgus producing intertrochanteric osteotomy for FNSF nonunion and assess the degree of Pauwels' correction, ON rate, and return to duty. Current functional outcomes were prospectively obtained via a telephone script. Six patients underwent Pauwels' osteotomy for FNSF nonunion, and all went onto bony union. Three of the six patients progressed onto ON, with two patients requiring a total hip arthroplasty due to life-limiting symptoms. Pauwels' osteotomy is a reliable salvage procedure for FNSF nonunions. (Journal of Surgical Orthopaedic Advances 29(4):234-239, 2020).
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January 2021

The therapeutic effect of Interleukin-18 on hypertrophic scar through inducing Fas ligand expression.

Authors:
Xin Le Wei-Wei Wu

Burns 2021 03 15;47(2):430-438. Epub 2020 Jul 15.

Department of Burn, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China; Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China. Electronic address:

Objective: Among downstream interleukin-18 (IL-18) targets, Fas ligand (FasL) in particular, has been strongly implicated in many conditions. Our study aims to explore the role of IL-18 in hypertrophic scar through enhancing FasL expression.

Methods: IL-18 expression in hypertrophic scar tissues and normal tissues were explored by immunohistochemistry, qRT-PCR and Western blotting, and the expression of IL-18 in normal skin fibroblasts and hypertrophic scar fibroblasts by immunofluorescence. Hypertrophic scar fibroblasts treated with recombinant human IL-18 (rhIL-18) were assessed with MTT, Annexin V-FITC/PI, qRT-PCR, ELISA and western blotting. In the hypertrophic scar of rabbit ears, rhIL-18 was injected to determine histological changes with HE and Masson staining. Additionally, the scars were rated based on contour and overall severity using a visual analog scale scores (VAS).

Results: IL-18 was decreased in hypertrophic scar tissues and fibroblasts compared to normal skin tissues and fibroblasts, respectively. Decreased proliferation and increased apoptosis of hypertrophic scar fibroblasts were found after rhIL-18 treatment with enhanced expression of FasL, sFasL FADD, Caspase-8, Caspase-9 and Caspase-3 in a dose-dependent manner. The VAS and thickness of scars in rabbit ears was decreased as time went on after rhIL-18 treatment, with decreases in scar elevation index (SEI) and the increases in FasL expression.

Conclusion: IL-18 curbs proliferation and promotes apoptosis of hypertrophic scar fibroblasts by enhancing FasL expression. IL-18is a potential target for treatment of hypertrophic scar.
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http://dx.doi.org/10.1016/j.burns.2020.07.008DOI Listing
March 2021

Spin-crossover in an organic-inorganic hybrid perovskite.

Chem Commun (Camb) 2020 Apr 23;56(33):4551-4554. Epub 2020 Mar 23.

Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, P. R. China.

The first spin-crossover (SCO) complex with an organic-inorganic hybrid perovskite structure (PPN)[Fe{Au(CN)}] (1) is reported, which displays three-step SCO behaviour. The light-induced excited spin-state trapping measurement gives T = 134 K for a three-dimensional FeL-type (L = bis-monodentate ligand) SCO complex. Moreover, spin-state dependent fluorescence is observed in 1.
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http://dx.doi.org/10.1039/d0cc00992jDOI Listing
April 2020

Maternal uniparental disomy of chromosome 4 indicated by allele copy number of short tandem repeats.

Forensic Sci Int Genet 2020 05 6;46:102273. Epub 2020 Mar 6.

Faculty of Forensic Medicine, Zhongshan Medical School of Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China. Electronic address:

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http://dx.doi.org/10.1016/j.fsigen.2020.102273DOI Listing
May 2020

Anteromedial thalamic nucleus to anterior cingulate cortex inputs modulate histaminergic itch sensation.

Neuropharmacology 2020 05 6;168:108028. Epub 2020 Mar 6.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica and Collaborative Innovation Center for Brain Science, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, China; Zhejiang Chinese Medical University, Hangzhou, 310053, China.

Itch is an unpleasant feeling that triggers scratching behavior. Much progress has been made in identifying the mechanism of itch at the peripheral and spinal levels, however, itch circuits in the brain remain largely unexplored. We previously found that anterior cingulate cortex (ACC) to dorsal medial striatum (DMS) inputs modulated histamine-induced itch sensation, but how itch information was transmitted to ACC remained unclear. Here, we demonstrated that the anteromedial thalamic nucleus (AM) was activated during histaminergic itch, and there existed reciprocal neuronal projections between AM and ACC. Disconnection between AM and ACC resulted in a significant reduction of histaminergic, but not nonhistaminergic, itch-related scratching behavior. Optogenetic activation of AM-ACC, but not ACC-AM, projections evoked histaminergic itch sensation. Thus, our studies firstly reveal that AM is critical for histaminergic itch sensation and AM-ACC projections modulate histaminergic itch-induced scratching behavior.
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http://dx.doi.org/10.1016/j.neuropharm.2020.108028DOI Listing
May 2020

A randomized trial of a distraction-type intervention to assist in managing dressing changes for children experienced burns.

J Adv Nurs 2020 Mar 15;76(3):878-887. Epub 2019 Dec 15.

Department of Burn Surgery, The First Hospital of Jilin University, Changchun, China.

Aim: To evaluate the impact of the specially designed medical dressing screen during wound dressing changes for children aged 1-3 who experienced a burn on their hand or foot.

Design: Randomized controlled trial.

Methods: The study was performed, between January 2019 - April 2019, at a Burn Outpatient Ward. A total of 52 outpatient children who had suffered burns were included in the clinical trial. The burn area of these participants accounted for 1-5% of the total body surface area. The children were randomly divided into two equal-sized groups, each receiving a different treatment. In the medical screen group (N = 26), a medical screen was used for children during the dressing changes. In the control group (N = 26), the children received only regular dressing changes. Pain level of the children during dressing change was the primary outcome, the satisfaction of children's parents and wound therapist were used as second outcomes. The Bonferroni method was used to perform pairwise comparisons of repeatedly measured data at different measurement times in two groups.

Results: The results showed that the medical screen group had better outcomes with respect to pain management during dressing changes; in addition, the satisfaction score of the wound therapist and children's parents presented also better outcomes compared with the control group.

Conclusion: This study demonstrated application of the medical screen for burns can relieve the pain of 1 - 3-year old children experienced a burn during dressing changes. In addition, the application of the medical screen also increased the satisfaction of the child's parents and wound therapist. Registration NO: 1,900,020,953.

Impact: Compared with conventional dressing methods, the medical screen can be used as a novel way to decrease the negative experience of burn patients ages 1-3 who require dressing changes.
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http://dx.doi.org/10.1111/jan.14278DOI Listing
March 2020

Impact of orally administered tramadol combined with self-selected music on adult outpatients with burns undergoing dressing change: A randomized controlled trial.

Burns 2020 06 29;46(4):850-859. Epub 2019 Oct 29.

Department of Burn Surgery, The First Hospital of Jilin University, Changchun 130021, China. Electronic address:

Objective: To investigate the effects of music and/or tramadol on pain and anxiety in burn outpatients undergoing dressing changes.

Design: Randomized controlled trial.

Setting: Burns and Plastic Reconstruction Unit.

Participants: Patients (N=180) with burns on up to 10%-30% of the total body surface area (TBSA).

Interventions: The patients were randomly allocated to 4 equal-sized groups as follows: (1) tramadol group (TG), patients received 100mg of tramadol orally 20min before the dressing change; (2) music group (MG), patients listened to self-selected music during the dressing change; (3) music-plus-tramadol group (MTG), patients received tramadol and listened to self-selected music; and (4) control group (CG), patients received a routine dressing change only. All patients underwent the interventions once per day for 2days.

Main Outcome Measures: McGill Pain Questionnaire Short Form (MPQ-SF) (primary outcome), McGill Pain Persian version of Burn Specific Pain Anxiety Scale (BSPAS) (primary outcome), and heart rate (HR) and overall patient satisfaction (secondary outcomes).

Results: The results showed that music-plus-tramadol group (MTG) had better outcomes with respect to pain and anxiety management during dressing changes.

Conclusions: In comparison with music or tramadol alone, the integration of music and tramadol offers a secure and favorable treatment choice to relieve pain and anxiety, ultimately improving the satisfaction levels of burn outpatients during dressing changes.
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http://dx.doi.org/10.1016/j.burns.2019.10.010DOI Listing
June 2020

Efficacy of acupuncture in the management of post-apoplectic aphasia: a systematic review and meta-analysis of randomized controlled trials.

BMC Complement Altern Med 2019 Oct 25;19(1):282. Epub 2019 Oct 25.

Department of Acupuncture, Anhui University of Chinese Medicine, Hefei, 230012, Anhui, China.

Background: Aim of this study was to evaluate the effectiveness of scalp, tongue, and Jin's 3-needle acupuncture for the improvement of postapoplectic aphasia.

Method: PubMed, Cochrane, Embase databases were searched using index words to identify qualifying randomized controlled trials (RCTs). Meta-analyses of odds ratios (OR) or standardized mean differences (SMD) were performed to evaluate the outcomes between investigational (scalp / tongue / Jin's 3-needle acupuncture) and control (traditional acupuncture; TA and/or rehabilitation training; RT) groups.

Results: Thirty-two RCTs (1310 participants in investigational group and 1270 in control group) were included. Compared to TA, (OR 3.05 [95% CI: 1.77, 5.28]; p<0.00001), tongue acupuncture (OR 3.49 [1.99, 6.11]; p<0.00001), and Jin's 3-needle therapy (OR 2.47 [1.10, 5.53]; p = 0.03) had significantly better total effective rate. Compared to RT, scalp acupuncture (OR 4.24 [95% CI: 1.68, 10.74]; p = 0.002) and scalp acupuncture with tongue acupuncture (OR 7.36 [3.33, 16.23]; p<0.00001) had significantly better total effective rate. In comparison with TA/RT, scalp acupuncture, tongue acupuncture, scalp acupuncture with tongue acupuncture, and Jin's three-needling significantly improved ABC, oral expression, comprehension, writing and reading scores.

Conclusion: As treatments to postapoplectic aphasia, scalp / tongue acupuncture and Jin's Three-needling are found better than TA and/or RT in yielding total effective rate and improving ABC, oral expression, comprehension, reading and writing scores.
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http://dx.doi.org/10.1186/s12906-019-2687-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815030PMC
October 2019

Computer-assisted needle trajectory planning and mathematical modeling for liver tumor thermal ablation: A review.

Math Biosci Eng 2019 05;16(5):4846-4872

College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China.

Radiofrequency ablation (RFA) and microwave ablation (MWA) have become an important means for treating liver tumors. RFA and MWA are a minimally invasive therapy which involves an ablation applicator or needle (i.e., radiofrequency electrode or microwave antenna) inserted percutaneously into a tumor under the guidance of medical imaging, so as to destroy the tumor in situ by heating-induced coagulation necrosis. Treatment planning, particularly needle trajectory planning, is crucial to RFA and MWA. In clinical procedures, however, needle trajectory planning still relies on the personal experience of clinicians. Manual needle trajectory planning is tedious and may cause inter-operator difference. Therefore, computer-assisted needle trajectory planning techniques are of clinical value and have been extensively explored. However, a literature review that focuses on computer-assisted needle trajectory planning for liver tumor RFA and MWA has not been reported. In this paper, we conducted an extensive review on computer-assisted needle trajectory planning for RFA and MWA of liver tumors. Fundamentals of needle trajectory planning are summarized. Algorithms for single-needle and multi-needle trajectory planning are analyzed. Shortcomings of current computer-assisted needle trajectory planning algorithms are discussed and future developments are suggested.
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http://dx.doi.org/10.3934/mbe.2019244DOI Listing
May 2019

Chiral Erbium(III) Complexes: Single-Molecule Magnet Behavior, Chirality, and Nuclearity Control.

Inorg Chem 2019 Aug 7;58(16):10694-10703. Epub 2019 Aug 7.

Key Laboratory of Bioinorganic and Synthetic Chemistry of the Ministry of Education, School of Chemistry , Sun Yat-Sen University , Guangzhou 510275 , P. R. China.

The reactions of chiral ligand ()/()-1,1'-binaphthyl-2,2'-diyl phosphate (-HL/-HL) and ErCl·6HO afford two pairs of di- and tetranuclear enantiomers [Er(/-L)(EtOH)]Cl·6.5EtOH (-, -) and [Er(PO)(/-L)(EtOH)(HO)]Cl(OH)·15EtOH·11HO (-, -). The nuclearity of these complexes is controllable and depends on the reaction temperature with a template effect. Their chirality was evidenced by circular dichroism (CD) spectra. - exhibits two magnetic relaxation pathways under a zero field, with an apparent barrier of 40 K. Ab initio calculations revealed a ferromagnetic dipolar interaction between these two Er(III) ions, the equatorial nature of the ligand field, and the probable origin of the two relaxations.
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http://dx.doi.org/10.1021/acs.inorgchem.9b00616DOI Listing
August 2019

Van der Waals Heterostructured MOF-on-MOF Thin Films: Cascading Functionality to Realize Advanced Chemiresistive Sensing.

Angew Chem Int Ed Engl 2019 Oct 22;58(42):14915-14919. Epub 2019 Aug 22.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences (CAS), 155 Yangqiao Road West, Fuzhou, Fujian, 350002, P. R. China.

Heterostructured metal-organic framework (MOF)-on-MOF thin films have the potential to cascade the various properties of different MOF layers in a sequence to produce functions that cannot be achieved by single MOF layers. An integration method that relies on van der Waals interactions, and which overcomes the lattice-matching limits of reported methods, has been developed. The method deposits molecular sieving Cu-TCPP (TCPP=5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin) layers onto semiconductive Cu-HHTP (HHTP=2,3,6,7,10,11-hexahydrotriphenylene) layers to obtain highly oriented MOF-on-MOF thin films. For the first time, the properties in different MOF layers were cascaded in sequence to synergistically produce an enhanced device function. Cu-TCPP-on-Cu-HHTP demonstrated excellent selectivity and the highest response to benzene of the reported recoverable chemiresistive sensing materials that are active at room temperature. This method allows integration of MOFs with cascading properties into advanced functional materials.
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http://dx.doi.org/10.1002/anie.201907772DOI Listing
October 2019

Survival analysis of intraoperative blood salvage for patients with malignancy disease: A PRISMA-compliant systematic review and meta-analysis.

Medicine (Baltimore) 2019 Jul;98(27):e16040

Department of Anesthesiology, West China Hospital.

Background: Intraoperative blood salvage as a blood-saving strategy has been widely used in surgery. Considering its theoretic risk of malignant tumor cells being reinfused and the corresponding blood metastases, the safety of intraoperative blood salvage in cancer surgery remains controversial.

Methods: Following the Preferred Reporting Items for Systemic Review and Meta-Analysis (PRISMA), we searched the Cochrane Library, MEDLINE and EMBASE to November 2017. We included only studies comparing intraoperative blood salvage with allogeneic blood transfusion.

Results: This meta-analysis included 9 studies with 4354 patients with 1346 patients in the intraoperative blood salvage group and 3008 patients in the allogeneic blood transfusion group. There were no significant differences in the 5-year overall survival outcome (odds ratio [OR] 1.12; 95% confidence interval [CI], 0.80-1.58), 5-year disease-free survival outcome (OR 1.08; 95% CI 0.86-1.35), or 5-year recurrence rate (OR 0.86; 95% CI 0.71-1.05) between the 2 study groups. Subgroup analysis also showed no significant differences in the 5-year overall survival outcome (OR 0.97; 95% CI 0.57-1.67) of hepatocellular carcinoma patients in liver transplantation.

Conclusions: For patients with malignant disease, intraoperative blood salvage did not increase the tumor recurrence rate and had comparable survival outcomes with allogeneic blood transfusion.
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http://dx.doi.org/10.1097/MD.0000000000016040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635293PMC
July 2019

A review of ultrasound detection methods for breast microcalcification.

Math Biosci Eng 2019 03;16(4):1761-1785

Department of Medical Imaging and Radiological Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.

Breast microcalcifications are one of the important imaging features of early breast cancer and are a key to early breast cancer diagnosis. Ultrasound imaging has been widely used in the detection and diagnosis of breast diseases because of its low cost, nonionizing radiation, and real-time capability. However, due to factors such as limited spatial resolution and speckle noise, it is difficult to detect breast microcalcifications using conventional B-mode ultrasound imaging. Recent studies show that new ultrasound technologies improved the visualization of microcalcifications over conventional B-mode ultrasound imaging. In this paper, a review of the literature on the ultrasonic detection methods of microcalcifications was conducted. The reviewed methods were broadly divided into high-frequency B-mode ultrasound imaging techniques, B-mode ultrasound image processing techniques, ultrasound elastography techniques, time reversal techniques, high spatial frequency techniques, second-order ultrasound field imaging techniques, and photoacoustic imaging techniques. The related principles and research status of these methods were introduced, and the characteristics and limitations of the various methods were discussed and analyzed. Future developments of ultrasonic breast microcalcification detection are suggested.
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http://dx.doi.org/10.3934/mbe.2019085DOI Listing
March 2019

Grazoprevir/elbasvir in peginterferon alfa plus ribavirin experienced patients with chronic genotype 1 HCV/HIV co-infection: a non-randomized, open-label clinical trial.

Infect Drug Resist 2019 18;12:937-945. Epub 2019 Apr 18.

Department of Internal Medicine, Division of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan City, Taiwan.

We aimed to evaluate the efficacy and tolerability of grazoprevir/elbasvir in patients with chronic genotype 1 hepatitis C virus (HCV) and HIV co-infection who experienced peginterferon alfa plus ribavirin (PegIFN/RBV) (clinicaltrials.gov NCT03098121). This non-randomized, open-label trial study was conducted in Taoyuan General Hospital. HIV-infected patients were screened for HCV antibody since June 1, 2012, and HCV and HIV co-infected patients were tested for HCV RNA. The subjects who experienced PegIFN/RBV were enrolled in the study, and of whom with chronic genotype 1a or 1b received grazoprevir 100 mg and elbasvir 50 mg in a fixed-dose combination tablet once daily with or without ribavirin for 12 to 16 weeks. Of 2,419 HIV-infected patients, 40 patients with chronic genotype 1 HCV and HIV co-infection who failed PegIFN/RBV treatment were enrolled. Sixteen patients had genotype 1a and 24 patients had genotype 1b, with or without cirrhosis. The median age was 42 (41-47) years, and 5 patients (12.5%) were diagnosed with liver cirrhosis (child Pugh score A). The median CD4 count was 504 cells/μL (321-689). All patients (100%) had HIV viral load <200 copies/mL, and HCV viral load was 6.3 log IU/mL (3.98-7.12). At the end of treatment, all patients (100%, 40/40) had undetectable HCV viral load, and 95.0% (38/40) of patients achieved sustained virologic response at 12 weeks. Grazoprevir/elbasvir was effective in genotype 1 patients co-infected with HIV with or without cirrhosis. This finding is consistent with that of previous trials of this regimen in monoinfected population.
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http://dx.doi.org/10.2147/IDR.S206938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489556PMC
April 2019

Application of Fluorescent Lymphography Technique in Lymphadenectomy of Gastrectomy.

JAMA Surg 2019 07;154(7):671-672

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.1001/jamasurg.2019.0156DOI Listing
July 2019

Bone Hardness of Different Anatomical Regions of Human Radius and its Impact on the Pullout Strength of Screws.

Orthop Surg 2019 Apr 25;11(2):270-276. Epub 2019 Mar 25.

Department of Orthopaedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: To investigate the bone hardness of different anatomical regions of the human radius and its impact on the pullout strength of screws.

Methods: Fresh radius bones were obtained from three donated cadavers. They were divided into three parts: proximal metaphysis, shaft, and distal metaphysis. The proximal metaphysis contains the head, neck, and radial tuberosity. The distal metaphysis includes the palmaris radius and the styloid process. The shaft of the radius was divided into nine segments of equal length. The bone hardness of three radiuses, one from each cadaver, was measured by Vickers microindentation hardness tests, and the screw pullout strength was examined in the other three radiuses using a materials testing machine. The trend between radius hardness and pullout strength was analyzed by using an analysis of variance randomized block design. Pearson correlation analysis was performed to evaluate the linear correlation between the bone hardness and the pullout strength of the human radius.

Results: The mean hardness ranged from 33.30 HV (the head) to 43.82 HV (the diaphysis). The hardest part of the radius was the shaft, with a value of 42.54 ± 5.59 HV. The proximal metaphysis had a hardness value of 34.15 ± 6.48 HV, and the distal metaphysis hardness value was 35.24 ± 5.17 HV. The shaft was 23.5% harder than the proximal metaphysis and 20% harder than the distal metaphysis. The microhardness test demonstrated that the bone hardness value of the diaphysis was significantly higher than those of both the proximal and distal metaphysis of the radius (both P < 0.05). The mean pullout strength values ranged from 552 N (the distal metaphysis) to 2296 N (the diaphysis). The greatest pullout strength of the radius was observed for the shaft, with a pullout strength of 1727.96 ± 111.44 N. The pullout strength of the proximal metaphysis was 726.33 ± 236.39 N, and the pullout strength of the distal metaphysis was 590.67 ± 36.30 N. The pullout strength of the shaft was 138% greater than that of the proximal metaphysis and 190% greater than that of the distal metaphysis. The pullout strength was also higher in the diaphysis than at both ends of the radius (both P < 0.05). A positive correlation was found between bone hardness and pullout strength (R = 0.927, P < 0.001).

Conclusions: Bone hardness and screw pullout strength are higher in the diaphysis of the radius than at either end. The pullout strength is positively related to bone hardness in the human radius.
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http://dx.doi.org/10.1111/os.12436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594527PMC
April 2019

Risk factors of the postoperative 30-day readmission of gastric cancer surgery after discharge: A PRISMA-compliant systematic review and meta-analysis.

Medicine (Baltimore) 2019 Mar;98(10):e14639

Department of Anesthesiology, West China Hospital.

Background: Readmission is a common postoperative adverse event. This study aimed to analyze potential risk factors for the incidence of postoperative 30-day readmission after discharge for gastric cancer patients with surgical treatment.

Methods: Those studies that reported the risk factors of gastric cancer patients who have a postoperative 30-day readmission were identified systematically from the PubMed, Cochrane, and Embase databases through July 2018. A systematic review and meta-analysis was performed to estimate the risk factors of postoperative 30-day readmission after gastric cancer surgery.

Results: Ultimately, 6 studies with 12,586 gastric cancer patients were included in the present study. There were 1473 (11.7%) patients who had postoperative 30-day readmission and 12,586 (88.3%) patients without 30-day postoperative readmission. A greater proportion of the readmission group had cardiovascular comorbidity (P < .001), pulmonary comorbidity (P < .001), and diabetes mellitus (P = .020) than the nonreadmission group. Furthermore, more patients in the readmission group had total gastrectomy (P < .001), combined organ resection (P < .001) and postoperative complications (P < .001) than did patients in the nonreadmission group. Nonhome discharge (odds ratio [OR] 1.580, P = .002), diabetes mellitus (OR 1.181, P = .044), postoperative complications (OR 2.656, P = .006), total gastrectomy (OR 2.242, P < .001), and combined organ resection (OR 1.534, P < .001) were independent risk factors for postoperative readmission.

Conclusion: Postoperative readmission is influenced by the synthetic action of preparative, intraoperative, and postoperative factors, such as diabetes mellitus, total gastrectomy, combined organ resection, nonhome discharge, and postoperative complications. Extra attention should be paid to those patients with high risk factors during the postoperative follow-up and recovery periods.
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http://dx.doi.org/10.1097/MD.0000000000014639DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417637PMC
March 2019

Clockwise, Modularized Lymphadenectomy in Laparoscopic Gastric Cancer Surgery: a New Laparoscopic Surgery Model.

J Gastrointest Surg 2019 05 23;23(5):895-903. Epub 2018 Oct 23.

Department of Gastrointestinal Surgery and Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No. 37 Guo Xue Xiang Street, Chengdu, Sichuan Province, China.

Background: The aim of the study is to present the clockwise, modularized lymphadenectomy model of laparoscopic gastrectomy for gastric cancer patients, which is based on our clinical practice experience in laparoscopic gastric cancer surgery.

Methods: From Jan 2015 to July 2017, 116 patients who underwent laparoscopic gastrectomy were retrospectively collected and analyzed. According to the different resection models, patients were divided into two groups: traditional laparoscopic lymphadenectomy group (63 patients) and clockwise, modularized lymphadenectomy group (53 patients). Operation-related parameters were compared between the two groups.

Results: The clockwise, modularized lymphadenectomy group had less dissection time (119.8 ± 19.1 min vs. 135.3 ± 23.8 min, p < 0.001) and less intraoperative blood loss (81.7 ± 42.9 ml vs. 91.4 ± 28.7 ml, p = 0.016) compared with the traditional laparoscopic lymphadenectomy group. Meanwhile, the clockwise, modularized lymphadenectomy group had more numbers of examined lymph nodes (40.5 ± 14.3 vs. 33.9 ± 11.0, p = 0.007) than the traditional laparoscopic lymphadenectomy group. Besides, there was no statistically significant difference in the postoperative complication rates between the two groups. The clockwise, modularized lymphadenectomy group had shorter postoperative hospital stay than the traditional laparoscopic lymphadenectomy group (8.7 ± 3.2 days vs. 10.4 ± 3.9 days, respectively, p < 0.001).

Conclusions: Through the adoption of the fixed sequence of lymphadenectomy, requirements and standard of lymphadenectomy of each lymph node station, and specific surgical skills for intraoperative exposure by the clockwise and modularized lymphadenectomy model, we can optimize and facilitate the laparoscopic gastric cancer surgery.
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http://dx.doi.org/10.1007/s11605-018-4009-1DOI Listing
May 2019

[A reduction technique for the treatment of refractory femoral trochanteric fracture].

Zhongguo Gu Shang 2018 Sep;31(9):867-870

No. 1 Hospital of Ninghai County, Ninghai 315600, Zhejiang, China;

Objective: To explore the effect of periosteal stripper through spiral blade incision combined with lateral sight bracket prying method in the treatment of irreducible femoral trochanteric fractures with proximal upwarping of fracture.

Methods: From March 2015 to October 2017, 26 patients with refractory femoral trochanteric fractures with proximal upwarp of fracture were treated by periosteal dissection through a spiral blade incision and lateral sight bracket prying, included 9 males and 17 females with an average age of (76.3±8.4) years old ranged from 63 to 85 years old. According to OA/OTA classification, 5 cases were type A1.3 fracture, 6 cases were A2.1, 7 cases were A2.2, 8 cases were A2.3. All cases were closed fractures. The amount of bleeding during operation and the time of fracture healing were observed and recorded. The Harris score of the hip joint function was performed.

Results: All cases were followed up from 7 to 26 months with an average of (17.4±4.7) months. Bone union time was from 9 to 15 months with an average of(12.4±3.5)weeks according to X-rays. At the final follow-up, Harris scores were 86 to 93 points with an average of (85.8±5.6) points, 23 cases were classified as excellent, 2 as good, and 1 as fair.

Conclusions: Using periosteum stripper through spiral blade incision combined lateral sight bracket prying for the treatment of irreducible femoral trochanteric fracture, the reduction effect is accurate, the operation time is shortened obviously, no auxiliary small incision is needed, the clinical effect is good, the reduction technique is simple and practical.
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http://dx.doi.org/10.3969/j.issn.1003-0034.2018.09.017DOI Listing
September 2018

Coexisting of bone marrow fibrosis, dysplasia and an X chromosomal abnormality in chronic neutrophilic leukemia with CSF3R mutation: a case report and literature review.

BMC Cancer 2018 03 27;18(1):343. Epub 2018 Mar 27.

Department of Hematology, Beijing Shijitan Hospital, Capital Medical University, Tieyi Road 10, Yangfangdian, Haidian District, Beijing, 100038, China.

Background: Chronic neutrophilic leukemia (CNL) is a rare myeloproliferative neoplasm (MPN) with less than 40 cases of patients being reported or clinically suspected meeting with 2008 World Health Organization ("WHO") diagnostic criteria. The current diagnosis of CNL remains to exclude other diseases. Recently, a new biomarker of CSF3R mutations that is almost invariably present in CNL has been identified. There is no effective treatment for CNL, therefore prognosis of the disease is poor, but it may be attributed to the presence of both SETBP1 and CSF3R gene mutations. The presence or absence of CSF3R mutation did not affect survival, whereas a trend for shortened survival was observed among patients with SETBP1-mutation.

Case Presentation: Here we report a 65-year old woman patient who presented with leukocytosis without sign of fever and tumors. Bone marrow aspirates showed a markedly hypercellular feature with 76%-92% myeloid and the dysplastic changes were found in about 7% of neutrophils cells. The bone marrow biopsy demonstrated marrow fibrosis with Gomori staining positive (+++~++++). Cytogenetic analysis showed 46,X,del (X) (q22). No molecular markers of BCR/ABL1 rearrangement (P210, P230, P190 and variably), JAK2V617F, FIP1L1-PDGFRA, TEL-PDGFRB, ZNF198-FGFR1 and SETBP1 mutations were identified, however, the CSF3R gene membrane proximal mutation (c.1853C > T/p.T618I sites) was detected by PCR techniques. The patient was diagnosed with CNL and died in about 2 months after disease diagnosis.

Conclusion: In clinical course, the CNL concurrently with severe bone marrow fibrosis and dysplastic features as well as X chromosomal abnormality may predict a worsening prognosis regardless of SETBP1 mutation status.
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http://dx.doi.org/10.1186/s12885-018-4236-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870516PMC
March 2018

Extracorporeal shock wave therapy with low-energy flux density inhibits hypertrophic scar formation in an animal model.

Int J Mol Med 2018 Apr 29;41(4):1931-1938. Epub 2018 Jan 29.

Burns and Plastic Reconstruction Unit, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Hypertrophic scar is characterized by excessive deposits of collagen during skin wound healing, which could become a challenge to clinicians. This study assessed the effects of the extracorporeal shock wave therapy (ESWT) on hypertrophic scar formation and the underlying gene regu-lation. A rabbit ear hypertrophic scar model was generated and randomly divided into three groups: L-ESWT group to receive L-ESWT (energy flux density of 0.1 mJ/mm2), H-ESWT (energy flux density of 0.2 mJ/mm2) and sham ESWT group (S-ESWT). Hypertrophic scar tissues were then collected and stained with hematoxylin and eosin (H&E) and Masson's trichrome staining, respectively, to assess scar elevation index (SEI), fibroblast density and collagen fiber arrangement. Expression of cell proliferation marker proliferating cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA) were assessed using RT-PCR and immunohistochemistry in hypertrophic scar tissues. H&E staining sections showed significant reduction of SEI and fibroblast density in both ESWT treatment groups compared to S-ESWT, but there was no dramatic difference between L-ESWT and H-ESWT groups. Masson's trichrome staining showed that collagen fibers were more slender and broader and oriented in parallel to skin surface after administration of ESWT compared to control tissues. At the gene level, PCNA‑positive fibroblasts and α-SMA-positive myofibroblasts were significantly decreased after L-ESWT or H-ESWT compared to the controls. Furthermore, there was no significant difference in expression of PCNA mRNA between L-ESWT or H-ESWT and S-ESWT, whereas expression of α-SMA mRNA significantly decreased in L-ESWT compared to that of H-ESWT and S-ESWT (P=0.002 and P=0.030, respectively). In conclusion, L-ESWT could be effective on suppression of hypertrophic scar formation by inhibition of scar elevation index and fibroblast density as well as α-SMA expression in hypertrophic scar tissues of the rabbit model.
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http://dx.doi.org/10.3892/ijmm.2018.3434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810209PMC
April 2018

Population structure of Han nationality in Central-Southern China.

Forensic Sci Int Genet 2017 07 4;29:e1-e3. Epub 2017 Apr 4.

Faculty of Forensic Medicine, Zhongshan Medical School of Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China. Electronic address:

Knowledge of population structure is very important for forensic genetics. However, the population substructure in Central-Southern China Han nationality has still not been fully described. In this study, we investigated the genetic diversity of 15 forensic autosomal STR loci from 6879 individuals in 12 Han populations subdivided by administrative provinces in Central-Southern China. The statistical analysis of genetic variation showed that genetic differentiation among these populations was very small with a F value of 0.0009. The Discriminant Analysis of Principal Components (DAPC) showed that there were no obvious population clusters in Central-Southern China Han population. In practice, the population structure effect in Central-Southern China Han population can be negligible in forensic identification and paternity testing.
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http://dx.doi.org/10.1016/j.fsigen.2017.04.001DOI Listing
July 2017

Potential of 13 linked autosomal short tandem repeat loci in pairwise kinship analysis.

Electrophoresis 2016 10 4;37(21):2800-2806. Epub 2016 Oct 4.

Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, P. R. China.

In this study, a panel of 13 STR loci locate on chromosome 3, 4, and 17 (D3S2402, D3S2452, D3S1766, D3S4554, D3S2388, D3S3051, D3S3053, D4S2404, D4S2364, AC001348A, AC001348B, D17S975, and D17S1294) were assessed for pairwise kinship analysis. Map distances between these STR loci ranged from 0.07 cM to 97.03 cM. The population genetic study of Chinese Han population showed that linkage disequilibrium exists in two clusters of closely linked markers (D4S2404-D4S2364 and D17S975-D17S1294), in which the recombination fractions were 0.0026 and 0.0001, respectively. The recombination fractions derived from the Rutgers Map for the closely linked markers (genetic distance < 0.5 cM) were significant underestimates in comparison to those of direct observation of STR transmissions in families. When effect of linkage on pairwise kinship testing was evaluated by comparing likelihood ratio (LR) values taking linkage into account, overall LR values increased. But extremely low LRs were also observed. Finally, the power of the 13 STR loci to discriminate relationship among full-sibs, half-sibs, grandparent-grandchild, uncle-niece, and unrelated pairs was assessed with a category fraction. The results showed that about 72.64% of full-sib pairs and about 82.84% of unrelated pairs could be classified correctly. But the category fractions of second-degree relationships drastically reduced to 7.34-35.48%. If only pairs of grandparent-grandchild, half-sibs, and uncle-niece were distinguished, the category fraction was 0.5512, 0.1147, and 0.4362, respectively. Our study results demonstrated that linked STRs were helpful to differentiate the most frequent relationships in pairwise kinship analysis.
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http://dx.doi.org/10.1002/elps.201600238DOI Listing
October 2016

Crossover Chimney Technique in an Impending Ruptured Aortic Dissection with Bilateral Iliac Tears.

J Vasc Interv Radiol 2016 Mar;27(3):451-3

Department of Vascular Surgery, Peking Union Medical College Hospital, No.1 Shuaifuyuan, Dongchen District, Beijing, China 100730; Department of Vascular Surgery, Beijing Tsinghua Changgung Hospital, Tsinghua University Medical Center, Beijing, China.

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http://dx.doi.org/10.1016/j.jvir.2015.11.048DOI Listing
March 2016

[Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci].

Fa Yi Xue Za Zhi 2015 Oct;31(5):373-6, 380

Objective: To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.

Methods: Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.

Results: A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.

Conclusion: The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.
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October 2015

Awakening from anesthesia using propofol or sevoflurane with epidural block in radical surgery for senile gastric cancer.

Int J Clin Exp Med 2015 15;8(10):19412-7. Epub 2015 Oct 15.

Department of Anesthesiology, The First Affiliated Hospital, Anhui Medical University Hefei 230032, China.

Objective: To study the awakening of the elderly patients from propofol intravenous general anesthesia or sevoflurane inhalation general anesthesia combined with epidural block after radical gastric cancer surgery.

Method: Eighty cases receiving selective radical surgery for gastric cancer were included. They were aged 65-78 years and classified as ASA grade I-II. Using a random number table, the cases were divided into 4 groups (n = 20): propofol intravenous general anesthesia (P group), sevoflurane inhalation general anesthesia (S group), propofol intravenous general anesthesia combined with epidural block (PE group), and sevoflurane inhalation general anesthesia combined with epidural block (SE group). For P and PE group, target controlled infusion of propofol was performed; for S and SE group, sevoflurane was inhaled to induce and maintain general anesthesia; for PE and SE group, before general anesthesia induction, epidural puncture and catheterization at T7-8 was performed. After surgery, perform patient controlled intravenous analgesia (PCIA) or patient controlled epidural analgesia (PCEA), and maintain VAS ≤ 3. The recorded indicators were as follows: time to recovery of spontaneous respiration, time to awakening, time of endotracheal tube removal, time to orientation, time to achieve modified Aldrete scores ≥ 9, modified OAA/S and Aldrete scores upon endotracheal tube removal (T1), 5 min after removal (T2), 15 min after removal (T3) and 30 min after removal (T4), dose of intraoperative remifentanil, intraoperative hypotension, and emergence agitation.

Results: Time to awakening, time of endotracheal tube removal, time to orientation, and time to achieve modified Aldrete scores ≥ 9 in PE and SE group were obviously shortened compared with P and S group (P < 0.05); modified OAA/S and Aldrete scores at T1 and T2 in PE and SE group were significantly higher than those in P and S group (P < 0.05), and the scores of SE group at T1 were much higher compared to PE group (P < 0.05). Dose of intraoperative remifentanil in PE and SE group was significantly lower than that in P and S group.

Conclusion: Compared to propofol intravenous general anesthesia or sevoflurane inhalation general anesthesia, propofol or sevoflurane general anesthesia combined with epidural block was more conducive to increasing the awakening quality of the senile patients from anesthesia after radical gastric cancer surgery. Moreover, sevofluorane inhalation general anesthesia combined with epidural block achieved a more stable hemodynamics and a shortened time to awakening.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694484PMC
January 2016