Publications by authors named "Wei-Guang Zhang"

89 Publications

Is aspirin a drug or just a marker for biliary cancer?

Hepatology 2021 Jul 9. Epub 2021 Jul 9.

Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The research by Liao et al. showed that postdiagnosis aspirin use improved the mortality of various subtypes of biliary tract cancer (BTC). The results were impressive, with the adjusted hazard ratios of mortality ranging from 0.51 to 0.58, and all subgroup analyses were statistically significant. However, we think that some other aspects should be discussed.
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http://dx.doi.org/10.1002/hep.32052DOI Listing
July 2021

Image quality and lesion detectability in low-dose pediatric F-FDG scans using total-body PET/CT.

Eur J Nucl Med Mol Imaging 2021 Mar 18. Epub 2021 Mar 18.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, China.

Purpose: To investigate the effects of dose reduction on image quality and lesion detectability of oncological F-FDG total-body PET/CT in pediatric oncological patients and explore the minimum threshold of administered tracer activity.

Methods: A total of 33 pediatric patients (weight 8.5-58.5 kg; age 0.8-17.6 years) underwent total-body PET/CT using uEXPLORER scanner with an F-FDG administered dose of 3.7 MBq/kg and an acquisition time of 600 s were retrospectively enrolled. Low-dose images (0.12-1.85 MBq/kg) were simulated by truncating the list-mode PET data to reducing count density. Subjective image quality was rated on a 5-point scale. Semi-quantitative uptake metrics for low-dose images were assessed using region-of-interest (ROI) analysis of healthy liver and suspected lesions and were compared with full-dose images. The micro-lesion detectability was compared among the dose-dependent PET images.

Results: Our analysis shows that sufficient subjective image quality and lesion conspicuity could be maintained down to 1/30th (0.12 MBq/kg) of the administered dose of F-FDG, where good image quality scores were given to 1/2- and 1/10- dose groups. The image noise was significantly more deranged than the overall quality and lesion conspicuity in 1/30- to 1/10-dose groups (all p < 0.05). With reduced doses, quantitative analysis of ROIs showed that SUV and SD in the liver increased gradually (p < 0.05), but SUV in the lesions and lesion-to-background ratio (LBR) showed no significant deviation down to 1/30-dose. One hundred percent of the F-FDG-avid micro-lesions identified in full-dose images were localized down to 1/15-dose images, while 97% of the lesion were localized in 1/30-dose images.

Conclusion: The total-body PET/CT might significantly decrease the administered dose upon maintaining the image quality and diagnostic performance of micro-lesions in pediatric patients. Data suggests that using total-body PET/CT, optimal image quality could be achieved with an administered dose-reduction down to 1/10-dose (0.37 MBq/kg).
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http://dx.doi.org/10.1007/s00259-021-05304-4DOI Listing
March 2021

Covalent Cross-Linking of Metal-Organic Cages: Formation of an Amorphous Cationic Porous Extended Framework for the Uptake of Oxo-Anions from Water.

Chempluschem 2021 Mar 18;86(5):699. Epub 2021 Mar 18.

School of Chemistry, South China Normal University, Guangzhou, 510006, P. R. China.

Invited for this month's cover are the collaborating groups of Sheng-Run Zheng and Wei-Guang Zhang from South China Normal University, China. The cover picture shows an amorphous cationic porous metal-organic material that constructed from the covalent linking of large cationic metal-organic cage for the removal of toxic oxo-anions from water with high capacities and rapid kinetics. Read the full text of the article at 10.1002/cplu.202000570.
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http://dx.doi.org/10.1002/cplu.202100056DOI Listing
March 2021

Covalent Cross-Linking of Metal-Organic Cages: Formation of an Amorphous Cationic Porous Extended Framework for the Uptake of Oxo-Anions from Water.

Chempluschem 2020 Nov 24;86(5):709-715. Epub 2020 Nov 24.

School of Chemistry, South China Normal University, Guangzhou, 510006, P. R. China.

Cationic amorphous metal-organic cage (MOC)-based materials capable of removing anionic pollutants from water are receiving increasing attention but they are still relatively less reported. Herein, for the first time, a cationic porous MOC-based extended framework, namely, CL-aMOC-1, was constructed by covalent linking of a cationic Pd L (L=3,5-di-pyridin-4-yl-benzaldehyde) cage with a 1,4-bis(4-aminophenyl)benzene (BAPB) linker. Interestingly, the reaction could be completed within 15 min using an amorphous MOC-based solid (aMOC-1) and BAPB as reactant via a low-temperature solid-state reaction. The CL-aMOC-1 showed improved stability, lower solubility and higher oxo-anion uptake in water compared with the original aMOC-1. The adsorption capacities for CrO , Cr O and ReO on CL-aMOC-1 were 245.1, 311.5 and 452.5 mg/g, respectively, in which the uptake of Cr(VI)-containing oxo-anions was among the highest compared with those of other metal-organic materials. The CL-aMOC-1 can selectively capture oxo-anions in the presence of competitive anions. It exhibits good reusability as over 85 % of the uptake capacity is retained after 5 cycles. Finally, it shows the ability to remove Cr(VI) ions from electroplating wastewater.
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http://dx.doi.org/10.1002/cplu.202000570DOI Listing
November 2020

Dihydromyricetin ameliorates chronic liver injury by reducing pyroptosis.

World J Gastroenterol 2020 Nov;26(41):6346-6360

Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

Background: Chronic liver injury (CLI) is now a worldwide disease. However, there is no effective treatment. Pyroptosis plays an essential role in CLI. Dihydromyricetin (DHM) resists oxidation and protects the liver. We hypothesize that the beneficial effect of DHM on CLI is related to its effect on the expression of pyroptosis-related molecules. Therefore, we studied the influence of DHM on CLI and pyroptosis.

Aim: To study the role of pyroptosis in the pathogenesis of CLI and the therapeutic mechanism of DHM.

Methods: Thirty-two mice were randomly divided into four groups: The control group was injected with olive oil, the carbon tetrachloride (CCl) group was injected with CCl, the vehicle group was injected with hydroxypropyl-β-cyclodextrin while injecting CCl and the DHM group was injected with DHM while injecting CCl. After four weeks of treatment, liver tissues from the mice were stained with hematoxylin and eosin, and oil red O. Blood was collected from the angular vein for serological analysis. The severity of CLI was estimated. Some liver tissue was sampled for immunohistochemistry, Western blotting and quantitative reverse transcription PCR to observe the changes in pyroptosis-related molecules.

Results: Serum total cholesterol, low density lipoprotein, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the CCl group were higher than those in the control group, and serum total cholesterol, low density lipoprotein, AST and ALT in the DHM group were lower than those in the vehicle group. Hematoxylin and eosin and oil red O staining showed that there were more lipid droplets in the CCl group than in the control group, and there were fewer lipid droplets in the DHM group than in the vehicle group. Western blotting showed that the expression of the pyroptosis-related molecules caspase-1, NOD-, LRR- and pyrin domain-containing 3 (NLRP3) and gasdermin D (GSDMD)-N in the CCl group was higher than that in the control group, while expression of these proteins in the DHM group was lower than that in the vehicle group. Quantitative reverse transcription PCR results showed that the expression of the pyroptosis-related genes caspase-1, NLRP3, GSDMD and interleukin-1β (IL-1β) in the CCl group was higher than that in the control group, while there was no significant change in NLRP3 and caspase-1 expression in the DHM group compared with that in the vehicle group, and the expression of GSDMD and IL-1β was decreased.

Conclusion: DHM improves CCl-induced CLI and regulates the pyroptosis pathway in hepatocytes. DHM may be a potential therapeutic agent for CLI.
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http://dx.doi.org/10.3748/wjg.v26.i41.6346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656208PMC
November 2020

[Clinical anatomical study on the treatment of carpal tunnel syndrome with classic Acupotomy].

Zhongguo Gu Shang 2020 Aug;33(8):745-9

Department of Acupuncture and Moxibustion, China-Japan Friendship Hospital, Beijing 100029, China.

Objective: To explore the safety of classic Acupotomy in the treatment of carpal tunnel syndrome.

Methods: Twenty six adult specimens (15 males and 11 females), aged 60 to 95(82.54±6.94) years old, were selected from 10% formalin antiseptic fixation. There were 52 sides(two of them could not be tested). The study period was from November 2017 to May 2018. The specimens were collected from the body donation center of the school of basic medicine, Peking University. The operation of releasing the transverse carpal ligament on the human body specimen was simulated by the classic acupotomy, and the distance from the four points to the surrounding anatomical structure was measured to calculate the direct injury rate to the nerve and blood vessels, and the shortest distance between the acupotomy and the nerve and blood vessels was defined as ≥2 mm as safety.

Results: In the experimental operation, the direct injury rate of nerve and blood vessel was 14% and 12% respectively. There was significant difference in the rate of direct nerve injury between the four injection points (<0.05). There was no significant difference in the rate of direct vascular injury between the four injection points (>0.05). Among the four points, there was a statistically significant difference in the safety of nerves(<0.05), and the safety of point 1 and point 3 of radial injection was higher than that of point 2 and point 4 of ulnar injection(<0.05). There was significant difference in the safety of blood vessels between the four points(<0.05), and the safety of radial point 1 was higher than that of ulnar point 2 and point 4 (<0.05).

Conclusion: The safety of the classic Acupotomy for carpal tunnel syndrome is related to the location of the needle entry point, and the safety of theradial proximal end of the needle is the highest.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2020.08.012DOI Listing
August 2020

A Mn(II)-MOF with inherent missing metal-ion defects based on an imidazole-tetrazole tripodal ligand and its application in supercapacitors.

Dalton Trans 2020 Sep;49(35):12150-12155

School of Chemistry, South China Normal University, Guangzhou, 510006, China. zhengsr-scnu.edu.cn wgzhang-scnu.edu.cn.

A metal-organic framework (MOF), namely SCNU-Z3, based on an imidazole-tetrazole tripodal ligand and Mn(ii), has been constructed. It exhibits a porous 3D framework composed of truncated octahedron cage subunits. Unexpected ligand-induced missing metal-ion defects were observed in the framework. In addition, the application of SCNU-Z3 in a supercapacitor was performed.
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http://dx.doi.org/10.1039/d0dt01666gDOI Listing
September 2020

N-methoxy-β-carboline alkaloids with inhibitory activities against Aβ aggregation and acetylcholinesterase from the stems of Picrasma quassioides.

Bioorg Chem 2020 08 25;101:104043. Epub 2020 Jun 25.

Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, People's Republic of China; College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address:

Nine new N-methoxy-β-carboline alkaloids (NMCAs) (1a/1b-3a/3b and 4-6) and two known NMCAs (7 and 8) were isolated from the stems of Picrasma quassioides. Their structures were elucidated by spectroscopic data analyses, quantum chemical calculations, and single-crystal X-ray crystallographic data. An analysis of the C NMR chemical shifts of the N-methoxy groups in these NMCAs and 41 gathered known compounds reveals the phenomenon that the chemical shifts of all these N-methoxy groups are greater than δ 62, which can be used to recognize the N-methoxy group rapidly. In addition, the acetylcholinesterase (AChE) and Aβ aggregation inhibitory activities of 1-8 were evaluated. Compounds 1, 2, 7, and 8 displayed AChE inhibitory activity with IC values of 14.9, 13.2, 17.6, and 43.9 μM, respectively. Compound 2 showed inhibition activity against Aβ aggregation with an IC value of 10.1 μM.
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http://dx.doi.org/10.1016/j.bioorg.2020.104043DOI Listing
August 2020

Sporormielones A-E, bioactive novel C-C coupled orsellinic acid derivative dimers, and their biosynthetic origin.

Chem Commun (Camb) 2020 Apr 25;56(33):4607-4610. Epub 2020 Mar 25.

Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China.

Sporormielones A-E (1-5), novel C-C coupled orsellinic acid derivative dimers containing tricyclic cores with a dimethylcyclopentenone unit, were obtained, of which 1-3 and 5 showed obvious short-term memory improvement activity in AD flies. Based on transcriptome analysis, C labelling, and gene deletion, their plausible biosynthetic mechanism was proposed.
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http://dx.doi.org/10.1039/d0cc00855aDOI Listing
April 2020

Anatomical and clinical study of a new mallet fracture classification method.

Chin Med J (Engl) 2020 Mar(6):657-663

Department of Hand Surgery, Beijing Jishuitan Hospital, Beijing 100035, China.

Background: Mallet fracture is avulsion of the terminal extensor tendon from the base of the distal phalangeal bone with a bony fragment. This study was performed to evaluate the anatomical characteristics of mallet fractures, investigate a new mallet fracture classification system using anatomical and imaging methods, and discuss the treatment schemes for different types of mallet fracture.

Methods: Sixty-four fresh cadaveric fingers were divided into four groups, and models of different types of mallet fracture with distal interphalangeal joint instability were established by dissecting 25%, 50%, 75%, and 100% of the bilateral collateral ligaments. The effect of mallet fractures on the stability of the distal interphalangeal joint was then observed. The lateral radiographs of mallet fractures in 168 patients were analyzed and classified according to the involvement of the joint surface in the fracture, the thickness of fracture, the untreated time after injury, and the complication of distal interphalangeal joint palmar subluxation. Forty-seven patients were surgically treated by reconstruction of extensor tendon insertion, the Ishiguro method, or single Kirschner wire fixation.

Results: The established mallet fracture model showed that the distal interphalangeal joint was stable when the bilateral collateral ligaments were cut off by 25% (t = -0.415, P = 0.684) and significantly unstable when this range was ≥50% (50% transection: t = -6.363, P < 0.001; 75% transection: t = -17.036, P < 0.001; 100% transection: t = -30.977, P < 0.001, respectively). The mallet fractures were divided into Types I, II, and III (fracture involving <20%, 20%-50%, and >50% of the joint surface, respectively). Type II was further divided into Types IIa and IIb according to whether the course of injury was < or ≥2 weeks, respectively. The mean post-operative flexion of the distal interphalangeal joint was 63.4° ± 7.9°, and the mean extension lag was 6.7° ± 4.6°.

Conclusions: The lateral collateral ligament is the main factor that maintains the stability of the distal interphalangeal joint. Classification that combines the involvement of the joint surface in the fracture, the thickness of the fracture, and the untreated time after injury is reasonable and will help to choose an appropriate operational method.
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http://dx.doi.org/10.1097/CM9.0000000000000676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190220PMC
March 2020

Rational design of ultra-small photoluminescent copper nano-dots loaded PLGA micro-vessels for targeted co-delivery of natural piperine molecules for the treatment for epilepsy.

J Photochem Photobiol B 2020 Apr 23;205:111805. Epub 2020 Jan 23.

Department of neurology, Mudanjiang Second Hospital, China.

In recent days, reported researches demonstrated that encapsulation of natural hydrophobic drug molecules (Piperine) into the biodegradable polymer system with nanoformulations opens a novel prospect in bio-nanomedicine field. Generally, the nanostructured materials embedded with the drug molecules could render enhanced efficiency in therapies. Piperine is a chief alkaloid compound of natural black pepper exhibits excellent anti-convulsant efficiency in the anti-epileptic treatment. Nonetheless, the poor water solubility of the piperine molecules has some difficulties in drug delivery and clinical applications. Herein we report the synthesis of Copper oxide quantum dots coated Hyaluronic acid (HA)/ Poly(lactic-co-glycolic acid) (PLGA) with for the effective delivery of piperine in the targeted drug delivery for epilepsy treatment. The physicochemical characterization was performed using the as prepared material. The crystal structure, surface morphology and the elemental composition were investigated from XRD, SEM, TEM and EDX analyses respectively. The surface morphology clearly stated the loading of CuO QDs loaded HA/PLGA microspheres. The capping of the polymer matrix was also studied using FTIR analysis. A Photoluminescence spectrum is also recorded. This study was illustrating that Piperine loaded [email protected]/PLGA nanostructures exhibit improved neuroprotection and encourage the activation of astrocytes in chemical kindling model of epilepsy. This proposed material could be a novel and effective therapeutic platform for the targeted drug delivery systems.
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http://dx.doi.org/10.1016/j.jphotobiol.2020.111805DOI Listing
April 2020

A new QCM signal enhancement strategy based on [email protected] framework complex for miRNA detection.

Anal Chim Acta 2020 Jan 19;1095:212-218. Epub 2019 Oct 19.

School of Chemistry, South China Normal University, Guangzhou, 510006, China. Electronic address:

Sensitive and selective detection of miRNA is of great significance for the early diagnosis of human diseases, especially for cancers. Quartz crystal microbalance (QCM) is an effective tool for detecting biological molecules; however, the application of QCM for miRNA detection is still very limited. One of the great needs for QCM detection is to further improve the QCM signal. Herein, for the first time, we promote a new signal enhancement strategy for the detection of miRNA by QCM. First, a hairpin biotin-modified DNA was used as a probe DNA, which exposes the biotin site when interacting with target miRNA. Then, a [email protected] framework ([email protected]) complex formed by electrostatic attractions between SA and a MOF was introduced into the QCM detection system. The [email protected] complexes serve as both a signal amplifier and a specific recognition element via specific biotin-SA interactions. The strategy was applied to the detection of a colorectal cancer marker, miR-221, by using a stable Zr(IV)-MOF, UiO-66-NH. The detection linear range was 10 fM-1 nM, the detection limit was 6.9 fM, and the relative standard deviation (RSD) (n = 5) was lower than 10% in both simulated conditions and the real serum environment. Furthermore, the detection limit reached 0.79 aM when coupled with the isothermal exponential amplification reaction (EXPAR).
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http://dx.doi.org/10.1016/j.aca.2019.10.034DOI Listing
January 2020

The interaction of an amorphous metal-organic cage-based solid (aMOC) with miRNA/DNA and its application on a quartz crystal microbalance (QCM) sensor.

Chem Commun (Camb) 2020 Jan 12;56(4):591-594. Epub 2019 Dec 12.

School of Chemistry, South China Normal University, Guangzhou, 510006, China. zhengsr-scnu.edu.cn wgzhang-scnu.edu.cn.

The interaction between an aMOC and miRNA/DNA is studied and the use of a signal probe DNA (spDNA)@aMOC complex as an effective amplifier in a QCM sensor to detect miRNA is developed. The signal can be significantly enhanced, which leads to improved performance when detecting and distinguishing miRNAs.
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http://dx.doi.org/10.1039/c9cc08014gDOI Listing
January 2020

Diagnostic efficacy of serum anti-phospholipase A2 receptor antibodies for idiopathic membranous nephropathy in patients with diabetic kidney disease.

Clin Chim Acta 2020 Mar 13;502:222-226. Epub 2019 Nov 13.

Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing, China. Electronic address:

Aim: Serum anti-phospholipase A2 receptor (anti-PLA2R) antibodies are highly accurate in diagnosing idiopathic membranous nephropathy (IMN) in populations with kidney disease. However, the diagnostic value of anti-PLA2R antibodies for IMN in diabetic kidney disease (DKD) is unclear. The objective of this study is to determine the diagnostic efficacy and the optimal cut-off value of this marker in populations with DKD.

Methods: This study included 227 patients with type 2 diabetes who were admitted to the Department of Nephrology of the Chinese People's Liberation Army General Hospital from May 2016 to January 2018 and underwent pathological diagnosis by renal biopsy. Anti-PLA2R antibodies were detected by enzyme-linked immunosorbent assay in this population. According to the pathological results, the participants were divided into an IMN group and non-membranous nephropathy (non-MN) group. The clinical characteristics were analyzed, the diagnostic ability of anti-PLA2R antibodies was evaluated, and the receiver operating characteristic (ROC) curve was constructed to obtain the optimal cut-off value.

Results: There were 45 patients in the IMN group, accounting for 19.8% of the study sample. The patients in this group were older at the time of renal biopsy than the non-MN group and presented a shorter duration of diabetes, better glycemic control, lower blood pressure and uric acid, and better renal function; in addition, their clinical symptoms indicated nephrotic syndrome. The optimal cut-off value for anti-PLA2R antibodies for the diagnosis of IMN in DKD was 2.71 Ru/ml, sensitivity was 0.800, specificity was 0.951, positive predictive value was 0.800, negative predictive value was 0.951, accuracy was 0.921, and the Yoden index was 0.750. The area under the ROC curve was 0.87 (95% CI, 0.788-0.952) (p < 0.001).

Conclusions: Patients in the IMN group were older, had better renal function and general condition, and the clinical symptoms indicated nephrotic syndrome. Anti-PLA2R antibodies had a good diagnostic performance for IMN in the population with DKD, and the optimal cut-off value was 2.71.
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http://dx.doi.org/10.1016/j.cca.2019.11.004DOI Listing
March 2020

Features of intra-hamate vascularity and its possible relationship with avascular risk of hamate fracture.

Chin Med J (Engl) 2019 Nov;132(21):2572-2580

Department of Hand Surgery, Beijing Jishuitan Hospital, Beijing 100035, China.

Background: The angiography with micro-computed tomography (micro-CT) has been proved its great advantages on investigating the intra-osseous vascularity of carpal bones. But few researches have focused on the intra-hamate vascularity. This study aimed to illustrate the intra-osseous arteries of the hamate and the relationship between the intra-hamate vascularity and the avascular risk of different types of hamate fractures.

Methods: Six normal cadaveric hamates were investigated with red lead (Pb3O4) micro-CT angiography. The intra-osseous arteries of specimens were clearly enhanced and the three-dimensional model was reconstructed. In order to study the features of the arterial entrances and intra-hamate vascularity, the diameters, quantities, locations of enhanced arteries, and the locations of transversal/proximal pole fracture lines on the body of the hamate were statistically compared. Besides, in order to analyze the relationship between intra-hamate vascularities and different hamate fractures, 127 cases of hamate fractures who presented in our hospital from March 2003 to June 2017 were retrospectively studied.

Results: A total of 94 cases were followed up (range: 4-37 months; mean: 12.4 months) effectively. The overall union rate of hamate fractures was as high as 92.6% (87 of 94 cases), while non-union of fracture on hamate hook was more common (P = 0.031). The arterial entrances were located around the dorsal, volar, radial, ulnar non-articular surfaces of the hamate body and the hook of the hamate. Generally, there were one to two trunk arteries on the volar non-articular surface and one to three trunk arteries on the dorsal non-articular surface. They formed one or two arterial arches, from which some branches were emitted and supplied the proximal parts. The intra-osseous vascularities of the hamate body were generally located in the radial part. The blood supply of the hook was mainly from the volar non-articular surface in most specimens. Hamate fractures could be classified into four types: fractures of the transversal/proximal pole, medial tuberosity, dorsal coronal of the hamate body, and fractures of the hamate hook.

Conclusions: This study showed new features of intra-hamate vascularity and the results will guide surgeons to reduce the vascular damage during the hamate fracture operations. The fracture lines of different types of hamate fractures may disrupt the intra-hamate arteries. The intra-hamate vascularities will have different influences on the avascular risks of different hamate fractures.
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http://dx.doi.org/10.1097/CM9.0000000000000417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6846259PMC
November 2019

An unprecedented 2D covalent organic framework with an htb net topology.

Chem Commun (Camb) 2019 Nov 24;55(89):13454-13457. Epub 2019 Oct 24.

School of Chemistry, South China Normal University, Guangzhou 510006, P. R. China.

A 2D imine-linked COF with a hitherto unreported htb type topology was synthesized from a linear diamine linker and a judiciously designed tetra-aldehyde building block. This work opens the door to the development of COFs with unprecedented topologies and may broaden the scope of COF functional materials by pore size and pore surface engineering.
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http://dx.doi.org/10.1039/c9cc06780aDOI Listing
November 2019

Homochiral Cu(I) Coordination Polymers Based on a Double-Stranded Helical Building Block from Achiral Ligands: Symmetry-Breaking Crystallization, Photophysical and Photocatalytic Properties.

Inorg Chem 2019 Nov 14;58(21):14660-14666. Epub 2019 Oct 14.

School of Chemistry , South China Normal University , Guangzhou , 510006 , P. R. China.

A pair of homochiral coordination polymers, [Cu(DPT)] ( and , HDPT = 3,5-di-4-pyridinyl-2H-tetrazole), were assembled from achiral precursors. Crystal structure analysis showed that they are chiral three-dimensional (3D) coordination polymers based on a new double-stranded helical building block that is composed of two different 1D helices. Interestingly, rare symmetry-breaking crystallization was observed, in which the possibility of obtaining enantio-enriched bulk product with excessive enantiomers () was obviously higher than that for enantiomers () as demonstrated in multiple, repeated experiments with single-crystal diffraction and vibrational circular dichroism (VCD) spectra. Moreover, compound [Cu(DPT)] shows good chemical stability in water, with pH values ranging from 3 to 13, as well as in many common organic solvents. Photophysical properties, including thermochromic properties and two-photon excited luminescence, were studied, and the potential for applications in temperature sensing was exhibited. In addition, the photocatalytic degradation of methylene blue in water indicated that compound [Cu(DPT)] can be used as a photocatalyst.
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http://dx.doi.org/10.1021/acs.inorgchem.9b02341DOI Listing
November 2019

An Anionic Nanotubular Metal-Organic Framework for High-Capacity Dye Adsorption and Dye Degradation in Darkness.

Inorg Chem 2019 Oct 4;58(20):13979-13987. Epub 2019 Oct 4.

School of Chemistry , South China Normal University , Guangzhou , 510006 , P. R. China.

A metal-organic framework (MOF), named SCNU-Z2, based on a new heterotopic tripodal nitrogen-containing ligand, has been constructed. Due to the replacement of one imidazole group in the reported ligand with one tetrazole group, the charge of the framework is changed from cationic to anionic but retains the same framework structure. The framework consists of tubular channels with a diameter of 1.5 nm and exhibits satisfactory stability in water with a pH range of 3-11. The anionic nature of the framework allows the effective adsorption of the cationic dyes MLB, CV, and RhB with capacities of 455.6, 847.4, and 751.8 mg/g, respectively. Among them, the adsorption capacities for SCNU-Z2 on CV and RhB rank as the highest when compared with other reported MOFs. In contrast, SCNU-Z2 exhibits an extremely low capacity for anionic dyes MO and AO, making it useful for the separation of anionic and cationic dyes based on a charge-dependent mode. Interestingly, SCNU-Z2 can be used to degrade an anionic dye, MB, within 30 min under darkness at room temperature. The apparent activation energy of the dye degradation reaction is calculated to be approximately 18.96 kJ·mol, implying that the catalytic reaction of MB can be considered as a low-temperature thermocatalytic reaction in the dark/SCNU-Z2 system.
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http://dx.doi.org/10.1021/acs.inorgchem.9b01959DOI Listing
October 2019

Integrin α6-Targeted Positron Emission Tomography Imaging of Colorectal Cancer.

ACS Omega 2019 Sep 11;4(13):15560-15566. Epub 2019 Sep 11.

Department of Endoscopy, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Department of Nuclear Medicine, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, and Department of Experiment, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.

Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer deaths worldwide. Integrin α6 is overexpressed in all stages of CRC which makes it a potential diagnostic biomarker for CRC. Previously, we identified an integrin α6-targeted peptide CRWYDENAC (dubbed RWY) using phage display technology and employed it for nasopharyngeal carcinoma specific nanotherapeutics. In this study, we developed a radiotracer, F-RWY, based on this integrin α6-targeted RWY peptide for positron emission tomography (PET) imaging of CRC. Integrin α6 was overexpressed on several CRC cells including HT29 cells where the biotin-labeled RWY peptide colocalized with integrin α6. F-RWY PET imaging was performed on subcutaneous, chemically induced, and genetically engineered CRC mice. F-RWY generated high PET signals in subcutaneous HT29 tumors, and the tumor uptake of F-RWY was reduced by a blocking study using nonradio-labeled RWY. Moreover, F-RWY PET imaging enabled detection of CRC in chemically induced and genetically engineered CRC mice. The overexpression of integrin α6 in tumor tissues isolated from chemically induced and genetically engineered CRC mice was confirmed. These results demonstrate the potential clinical application of F-RWY for PET imaging of CRC.
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http://dx.doi.org/10.1021/acsomega.9b01920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761804PMC
September 2019

Integrin α6 targeted positron emission tomography imaging of hepatocellular carcinoma in mouse models.

J Control Release 2019 09 7;310:11-21. Epub 2019 Aug 7.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. Electronic address:

Integrin α6 emerges to be a diagnostic biomarker for hepatocellular carcinoma (HCC). Here, we translated our previously identified integrin α6 targeted peptide RWY into a positron emission tomography (PET) tracer F-RWY for the detection of HCC lesions in following four HCC mouse models including subcutaneous, orthotopic, genetically engineered and chemical induced HCC mice. F-RWY produced high PET signals in liver tumor tissues that were reduced by blocking studies using nonradiolabeled RWY peptide. We compared the integrin α6 targeted PET tracer F-RWY with the integrin αvβ3-targeted PET tracer F-3PRGD and the clinical PET tracer F-FDG in chemical induced HCC mice. Among 12 HCC identified by enhanced magnetic resonance imaging (MRI) with hepatocellular specific gadoxetate disodium Gd-EOB-DTPA, the sensitivities of F-RWY, F-3PRGD and F-FDG were approximately 92%, 73% and 50% while the tumor-to-liver ratios were 4.36 ± 1.41, 1.97 ± 0.43 and 1.63 ± 0.23 respectively. Additionally, PET imaging with the integrin α6 targeted F-RWY enabled to visualize small HCC lesions with diameters approximately 0.2 cm that was hard to be distinguished from surround hepatic vascular by enhanced MRI with Gd-EOB-DTPA. These findings potentiate the use of integrin α6 targeted PET tracer F-RWY for the detection of HCC.
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http://dx.doi.org/10.1016/j.jconrel.2019.08.003DOI Listing
September 2019

Hemodynamic changes in hepatic sinusoids of hepatic steatosis mice.

World J Gastroenterol 2019 Mar;25(11):1355-1365

Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

Background: Fatty liver (FL) is now a worldwide disease. For decades, researchers have been kept trying to elucidate the mechanism of FL at the molecular level, but rarely involve the study of morphology and medical physics. Traditionally, it was believed that hemodynamic changes occur only when fibrosis occurs, but it has been proved that these changes already show in steatosis stage, which may help to reveal the pathogenesis and its progress. Because the pseudolobules are not formed during the steatosis stage, this phenomenon may be caused by the compression of the liver microcirculation and changes in the hemodynamics.

Aim: To understand the pathogenesis of hepatic steatosis and to study the hemodynamic changes associated with hepatic steatosis.

Methods: Eight-week-old male C57BL/6 mice were divided into three groups randomly (control group, 2-wk group, and 4-wk group), with 16 mice per group. A hepatic steatosis model was established by subcutaneous injection of carbon tetrachloride in mice. After establishing the model, liver tissue from mice was stained with hematoxylin and eosin (HE), and oil red O stains. Blood was collected from the angular vein, and hemorheological parameters were estimated. A two-photon fluorescence microscope was used to examine the flow properties of red blood cells in the hepatic sinusoids.

Results: Oil red O staining indicated lipid accumulation in the liver after CCl treatment. HE staining indicated narrowing of the hepatic sinusoidal vessels. No significant difference was observed between the 2-wk and 4-wk groups of mice on morphological examination. Hemorheological tests included whole blood viscosity (mPas, γ = 10 s-/γ = 100 s) (8.83 ± 2.22/4.69 ± 1.16, 7.73 ± 2.46/4.22 ± 1.32, and 8.06 ± 2.88/4.22 ± 1.50), red blood cell volume (%) (51.00 ± 4.00, 42.00 ± 5.00, and 40.00 ± 3.00), the content of plasma fibrinase (g/L) (3.80 ± 0.50, 2.90 ± 0.80, and 2.30 ± 0.70), erythrocyte deformation index (%) (44.49 ± 5.81, 48.00 ± 15.29, and 44.36 ± 15.01), erythrocyte electrophoresis rate (mm/s per V/m) (0.55 ± 0.11, 0.50 ± 0.11, and 0.60 ± 0.20), revealing pathological changes in plasma components and red blood cells of hepatic steatosis. Assessment of blood flow velocity in the hepatic sinusoids with a laser Doppler flowmeter (mL/min per 100 g) (94.43 ± 14.64, 80.00 ± 12.12, and 67.26 ± 5.92) and two-photon laser scanning microscope (μm/s) (325.68 ± 112.66, 213.53 ± 65.33, and 173.26 ± 44.02) revealed that as the modeling time increased, the blood flow velocity in the hepatic sinusoids decreased gradually, and the diameter of the hepatic sinusoids became smaller (μm) (10.28 ± 1.40, 6.84 ± 0.93, and 5.82 ± 0.79).

Conclusion: The inner diameter of the hepatic sinusoids decreases along with the decrease in the blood flow velocity within the sinusoids and the changes in the systemic hemorheology.
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http://dx.doi.org/10.3748/wjg.v25.i11.1355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429340PMC
March 2019

Stable Hydrazone-Linked Covalent Organic Frameworks Containing O,N,O'-Chelating Sites for Fe(III) Detection in Water.

ACS Appl Mater Interfaces 2019 Apr 22;11(13):12830-12837. Epub 2019 Mar 22.

School of Chemistry and Environment , South China Normal University , Guangzhou 510006 , P. R. China.

Two stable crystalline hydrazone-linked covalent organic frameworks (COFs) (Bth-Dha and Bth-Dma) containing functional O,N,O'-chelating sites have been designed and successfully synthesized by the Schiff-base condensation reactions between benzene-1,3,5-tricarbohydrazide (Bth) and 2,5-dihydroxyterephthalaldehyde (Dha) or 2,5-dimethoxyterephthal-aldehyde (Dma), respectively. Bth-Dma exhibits strong fluorescence in the solid state and in an aqueous dispersion, while no fluorescence can be observed for Bth-Dha. Interestingly, the as-synthesized Bth-Dma can be used as a turn-off fluorescence sensor for the Fe(III) ion in aqueous solution with outstanding selectivity and sensitivity. The recognition process can be attributed to the coordination interaction between Fe(III) ion and the O,N,O'-chelating sites in the pore wall of Bth-Dma COF, as verified by X-ray photoelectron spectroscopy and H NMR spectroscopy. To the best of our knowledge, this is the first report on the rational design of luminescent COF with predesigned O,N,O'-chelating sites as a fluorescence sensor for highly selective and sensitive metal ion detection. This work may pave the way for designing luminescent COF sensors with functional binding sites for detecting specific metal ions.
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http://dx.doi.org/10.1021/acsami.9b02640DOI Listing
April 2019

Peroxisome Proliferator-Activated Receptor-γ Coactivator-1α Inhibits Vascular Calcification Through Sirtuin 3-Mediated Reduction of Mitochondrial Oxidative Stress.

Antioxid Redox Signal 2019 07 2;31(1):75-91. Epub 2019 Apr 2.

1 Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China.

Vascular calcification is associated with cardiovascular death in patients with chronic kidney disease (CKD). Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) plays an important role in various cardiovascular diseases. However, its role in vascular calcification remains unknown. Adenine-induced rat CKD model was used to induce arterial medial calcification. The level of PGC-1α decreased in abdominal aorta of CKD rats. Overexpression of PGC-1α significantly ameliorated calcium deposition in rat abdominal aorta, isolated carotid rings, and cultured vascular smooth muscle cells (VSMCs). Mitochondrial reactive oxygen species (mtROS) increased in calcifying aorta and VSMCs. Upregulation of PGC-1α inhibited, whereas PGC-1α depletion promoted β-glycerophosphate-induced mtROS production and calcium deposition. Moreover, PGC-1α increased superoxide dismutase 1 (SOD1) and SOD2 contents and , whereas SOD2 deletion eliminated PGC-1α-mediated mtROS change and promoted calcium deposition. Mechanistically, sirtuin 3 (SIRT3) expression declined in calcifying aorta and VSMCs, while PGC-1α overexpression restored SIRT3 expression. Inhibition of SIRT3 by 3-TYP or siRNA (small interfering RNA) reduced PGC-1α-induced upregulation of SOD1 and SOD2, and abolished the protective effect of PGC-1α on calcification of VSMCs. Importantly, PGC-1α was reduced in calcified femoral arteries in CKD patients. In phosphate-induced human umbilical arterial calcification, upregulation of PGC-1α attenuated calcium nodule formation, while this protective effect was abolished by SIRT3 inhibitor. We showed for the first time that PGC-1α is an important endogenous regulator against vascular calcification. Induction of PGC-1α could be a potential strategy to treat vascular calcification in CKD patients. PGC-1α protected against vascular calcification by SIRT3-mediated mtROS reduction.
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http://dx.doi.org/10.1089/ars.2018.7620DOI Listing
July 2019

Hydrolytically Stable Nanotubular Cationic Metal-Organic Framework for Rapid and Efficient Removal of Toxic Oxo-Anions and Dyes from Water.

Inorg Chem 2019 Feb 5;58(4):2899-2909. Epub 2019 Feb 5.

School of Chemistry and Environment , South China Normal University , Guangzhou , 510006 , PR China.

Cationic framework materials capable of removing anionic pollutants from wastewater are highly desirable but relatively rarely reported. Herein, a cationic MOF (SCNU-Z1-Cl) possessing tubular channels with diameter of 1.5 nm based on Ni(II) and a nitrogen-containing ligand has been synthesized and applied to capture hazardous anionic contaminants from water. The SCNU-Z1-Cl exhibits high BET surface area of 1636 m/g, and shows high hydrolytically stability in pH range from 4 to 10. Owing to the large tubular channels and the uncoordinated anions in the framework, the aqueous-phase anion-exchange applications of SCNU-Z1-Cl were explored with environmentally toxic oxo-anions including CrO, CrO, MnO, and ReO, and organic dyes. The adsorption of oxoanions exhibits high uptake kinetics and the adsorption capacities of CrO, CrO, MnO, and ReO are 126, 241, 292, and 318 mg/g, respectively, which were some of the highest values in the field of MOF/COF. In additional, the selectively is high when other concurrent anions are exist. The anionic dyes with different sizes including methyl orange, acid orange A, congo red, as well as methyl blue can be adsorbed by SCNU-Z1-Cl in few minutes to about 1 h. The adsorption capacities for them are 285, 180, 585, and 262 mg/g, respectively. In contrast, the adsorption kinetics for catinionic dyes with different sizes is obviously lower and exhibit a size-selectively adsorption that only cationic dye with suitable size (rhodamine B) can be adsorbed by SCNU-Z1-Cl. Consequently, SCNU-Z1-Cl can sepearate organic dyes in three different modes: size-dependent, charge-dependent, and kinetics-dependent selective adsorption. The excellent adsorption and separation properties of SCNU-Z1-Cl is attribute to the cationic framework, large tubular channel, as well as the high positive Zeta potential.
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http://dx.doi.org/10.1021/acs.inorgchem.9b00104DOI Listing
February 2019

promotes regeneration after sciatic nerve crush injury in rats.

Neural Regen Res 2019 Apr;14(4):683-691

Department of Trauma and Orthopedics, Peking University People's Hospital, Beijing, China.

Qian-Zheng-San, a traditional Chinese prescription consisting of Typhonii Rhizoma, Bombyx Batryticatus, Scorpio, has been found to play an active therapeutic role in central nervous system diseases. However, it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury. Therefore, we used Sprague-Dawley rats to investigate this. A sciatic nerve crush injury model was induced by clamping the right sciatic nerve. Subsequently, rats in the treatment group were administered 2 mL Qian-Zheng-San (1.75 g/mL) daily as systemic therapy for 1, 2, 4, or 8 weeks. Rats in the control group were not administered Qian-Zheng-San. Rats in sham group did not undergo surgery and systemic therapy. Footprint analysis was used to assess nerve motor function. Electrophysiological experiments were used to detect nerve conduction function. Immunofluorescence staining was used to assess axon counts and morphological analysis. Immunohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord. At 2 and 4 weeks postoperatively, the sciatic nerve function index, nerve conduction velocity, the number of distant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group. At 2 weeks postoperatively, nerve fiber diameter, myelin thickness, and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group. These results indicate that Qian-Zheng-San has a positive effect on peripheral nerve regeneration.
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http://dx.doi.org/10.4103/1673-5374.247472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352607PMC
April 2019

Plexiform fibromyxoma of the small bowel: A case report.

World J Clin Cases 2018 Dec;6(15):1067-1072

Department of Endoscopy, the Affiliated Hospital of Guizhou Medical University, Guiyang 550001, Guizhou Province, China.

Background: Plexiform fibromyxoma is a rare, special type of mesenchymal tumor. The most common presenting symptoms are anemia, hematemesis, and hematochezia, without sex or age predilection. The reported cases have mainly occurred in the gastric antrum and pylorus region, with some cases in the duodenum.

Case Summary: We report here a case of plexiform fibromyxoma in the upper segment of the jejunum, which was continuously followed up for 3 years after surgical removal. Plexiform fibromyxoma showed multinodular or plexiform growth. The cells in the tumor node were spindle-shaped but few in number and mitotic figures. Small blood vessels and mucous matrix were found among the tumor cells. Immunohistochemistry revealed that the plexiform fibromyxoma cells were positive for smooth muscle actin, focally positive for CD10, and negative for cytokeratin, CD117, DOG-1 (discovered on GIST-1) desmin, S-100, epithelial membrane antigen, and CD34. Ki-67 labeling index was < 5%. Plexiform fibromyxoma showed benign biological behavior. After 3 years of consecutive postoperative follow-up, no obvious signs of metastasis or recurrence were found by imaging examination.

Conclusion: Plexiform fibromyxoma is a rare type of mesenchymal tumor. The diagnosis mainly depends on pathological examination, and it should be distinguished from other gastrointestinal mesenchymal tumors.
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http://dx.doi.org/10.12998/wjcc.v6.i15.1067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288503PMC
December 2018

Clinicopathological Features of Nondiabetic Renal Diseases from Different Age Groups: An Observational Cross-sectional Study.

Chin Med J (Engl) 2018 Dec;131(24):2953-2959

Department of Nephrology, Chinese People's Liberation Army General Hospital, Chinese People's Liberation Army Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853, China.

Background: Diabetes mellitus (DM) has become the leading cause of chronic kidney disease (CKD). Nondiabetic renal diseases (NDRDs) have different clinicopathological features and prognosis from those of diabetic nephropathy. Our study sought to analyze the clinical and pathological features of NDRDs, in different age groups through a cross-sectional study.

Methods: All patients with type 2 DM at our center who underwent renal biopsy between March 1997 and March 2017 were screened and divided into three groups by age: Group 1 (youth group), 18-44 years old; Group 2 (middle-aged group), 45-59 years old; and Group 3 (elderly group), ≥60 years old. We analyzed the clinicopathological data and risk factors by univariate and multivariate logistic regression for NDRD of the patients to identify the features of NDRD in different age groups.

Results: We included 982 patients in the final analysis. Patients with NDRD accounted for 64.4% of all patients. IgA nephropathy (IgAN) was the most common pathological pattern in young patients with NDRD, accounting for 26.3%. In the middle-aged group, the two most common pathological patterns were IgAN and membranous nephropathy. Membranous nephropathy was the most common pathological pattern in elderly patients with NDRD, accounting for 29.3%. Consistent with pathological features, glomerular hematuria is a risk factor for NDRD in Group 1 (odds ratio [OR], 26.514; 95% confidence interval [CI], 2.503-280.910; P = 0.006). On the other hand, rapidly increasing proteinuria or nephrotic syndrome is a risk factor for NDRD in Group 2 (OR, 5.921; 95% CI, 2.061-17.013; P = 0.001) and Group 3 (OR, 90.409; 95% CI, 6.198-1318.826; P = 0.001).

Conclusions: This single-center study showed that the proportion and composition of NDRD differ among different age groups. Consistent with pathological features, some clinical indices such as hematuria and proteinuria showed different features among different age groups.
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http://dx.doi.org/10.4103/0366-6999.247197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302642PMC
December 2018

Assembly of a miRNA-modified QCM sensor for miRNA recognition through response patterns.

J Mol Recognit 2019 05 6;32(5):e2772. Epub 2018 Dec 6.

School of Chemistry and Environment, South China Normal University, Guangzhou, China.

In this paper, a miRNA-based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR-27a, miR-27b, and Let-7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR-27a and miR-27b. The selective sensing of miR-27a in mixed miRNA solution was also achieved. This miRNA-based QCM biosensor has the advantages of real-time, label-free, and short cycle detection.
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http://dx.doi.org/10.1002/jmr.2772DOI Listing
May 2019