Publications by authors named "Wei Zhou"

4,348 Publications

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Large-Scale Studies on Antimicrobial Resistance and Molecular Characterization of Escherichia coli from Food Animals in Developed Areas of Eastern China.

Microbiol Spectr 2022 Aug 11:e0201522. Epub 2022 Aug 11.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.

Widely distributed multidrug-resistant (MDR) bacteria threaten animals and human health. Nevertheless, few antimicrobial resistance (AMR) surveys of large-scale animal-derived bacteria have been explored. Here, 1,468 (97.54%) Escherichia coli strains were isolated from 1,505 pig (1,060) and chicken (445) anal swab samples from 11 cities in Zhejiang Province, China, in 2020. These isolates had a high resistance to tetracycline (92.92%), sulfisoxazole (93.05%), florfenicol (83.11%), and ampicillin (78.27%). More than 88.68% of the strains were MDR bacteria. A low AMR ratio to the "last-resort" antimicrobials tigecycline (0.75%), colistin (1.36%), and meropenem (0.75%) were found. The AMR of E. coli from pigs was higher than that of chickens. Eighteen strains among 31 MDR strains that were resistant to "last-resort" antimicrobials could transfer the AMR genes (, (X), and ) to the recipient strain J53, which confer colistin, tigecycline, and carbapenem resistance, respectively. The homology among -carrying isolates was relatively high, and the sequence types were mainly ST5529, ST101, and ST354, while the homology of isolates harboring (X4) and genes were different. The , , and (X4) genes in strains LS45, JH51, and TZ118 were identified on the Incl2, IncHI2, and IncX1 plasmids, respectively. Moreover, (A), , and were the most common ARGs in 31 strains. Additionally, the heavy metals copper and zinc had a significant correlation with amoxicillin/clavulanate and tetracycline resistance. Controlling the movement of animals between cities and reducing the use of antimicrobials are effective methods to reduce the threat of AMR bacteria. Pigs and chickens are the most common food animals that are the important vectors for spreading antimicrobial-resistant pathogens among animals and humans. Limited systematic AMR monitoring of these food animal origin bacteria had been reported, especially in developed areas of China. Our study provides a comprehensive and systematic study of AMR in Escherichia coli from eastern China. The AMR of E. coli strains among the animals or cities has statistically significant differences. Moreover, the , (X4), and genes, considered resistant to the last line of AMR, were identified in part of farms. The transferability and the prevalence of these AMR strains were intensively studied. Our monitoring is comparable to human clinical research and has an essential reference for public health safety. These findings will provide early warning for AMR strains and guide the clinical use of antibiotics to control the spread of antibiotic resistance.
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http://dx.doi.org/10.1128/spectrum.02015-22DOI Listing
August 2022

Effect of a Decision Aid on Agreement Between Patient Preferences and Repair Type for Abdominal Aortic Aneurysm: A Randomized Clinical Trial.

JAMA Surg 2022 Aug 10:e222935. Epub 2022 Aug 10.

Department of Surgery and VA Outcomes Group, White River Junction VA Medical Center, White River Junction, Vermont.

Importance: Patients with abdominal aortic aneurysm (AAA) can choose open repair or endovascular repair (EVAR). While EVAR is less invasive, it requires lifelong surveillance and more frequent aneurysm-related reinterventions than open repair. A decision aid may help patients receive their preferred type of AAA repair.

Objective: To determine the effect of a decision aid on agreement between patient preference for AAA repair type and the repair type they receive.

Design, Setting, And Participants: In this cluster randomized trial, 235 patients were randomized at 22 VA vascular surgery clinics. All patients had AAAs greater than 5.0 cm in diameter and were candidates for both open repair and EVAR. Data were collected from August 2017 to December 2020, and data were analyzed from December 2020 to June 2021.

Interventions: Presurgical consultation using a decision aid vs usual care.

Main Outcomes And Measures: The primary outcome was the proportion of patients who had agreement between their preference and their repair type, measured using χ2 analyses, κ statistics, and adjusted odds ratios.

Results: Of 235 included patients, 234 (99.6%) were male, and the mean (SD) age was 73 (5.9) years. A total of 126 patients were enrolled in the decision aid group, and 109 were enrolled in the control group. Within 2 years after enrollment, 192 (81.7%) underwent repair. Patients were similar between the decision aid and control groups by age, sex, aneurysm size, iliac artery involvement, and Charlson Comorbidity Index score. Patients preferred EVAR over open repair in both groups (96 of 122 [79%] in the decision aid group; 81 of 106 [76%] in the control group; P = .60). Patients in the decision aid group were more likely to receive their preferred repair type than patients in the control group (95% agreement [93 of 98] vs 86% agreement [81 of 94]; P = .03), and κ statistics were higher in the decision aid group (κ = 0.78; 95% CI, 0.60-0.95) compared with the control group (κ = 0.53; 95% CI, 0.32-0.74). Adjusted models confirmed this association (odds ratio of agreement in the decision aid group relative to control group, 2.93; 95% CI, 1.10-7.70).

Conclusions And Relevance: Patients exposed to a decision aid were more likely to receive their preferred AAA repair type, suggesting that decision aids can help better align patient preferences and treatments in major cardiovascular procedures.

Trial Registration: ClinicalTrials.gov Identifier: NCT03115346.
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http://dx.doi.org/10.1001/jamasurg.2022.2935DOI Listing
August 2022

Enhanced rare disease mapping for phenome-wide genetic association in the UK Biobank.

Genome Med 2022 Aug 9;14(1):85. Epub 2022 Aug 9.

Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI, USA.

Background: Rare diseases collectively affect up to 10% of the population, but often lack effective treatment, and typically little is known about their pathophysiology. Major challenges include suboptimal phenotype mapping and limited statistical power. Population biobanks, such as the UK Biobank, recruit many individuals who can be affected by rare diseases; however, investigation into their utility for rare disease research remains limited. We hypothesized the UK Biobank can be used as a unique population assay for rare diseases in the general population.

Methods: We constructed a consensus mapping between ICD-10 codes and ORPHA codes for rare diseases, then identified individuals with each rare condition in the UK Biobank, and investigated their age at recruitment, sex bias, and comorbidity distributions. Using exome sequencing data from 167,246 individuals of European ancestry, we performed genetic association controlling for case/control imbalance (SAIGE) to identify potential rare pathogenic variants for each disease.

Results: Using our mapping approach, we identified and characterized 420 rare diseases affecting 23,575 individuals in the UK Biobank. Significant genetic associations included JAK2 V617F for immune thrombocytopenic purpura (p = 1.24 × 10) and a novel CALR loss of function variant for essential thrombocythemia (p = 1.59 × 10). We constructed an interactive resource highlighting demographic information ( http://www-personal.umich.edu/~mattpat/rareDiseases.html ) and demonstrate transferability by applying our mapping to a medical claims database.

Conclusions: Enhanced disease mapping and increased power from population biobanks can elucidate the demographics and genetic associations for rare diseases.
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http://dx.doi.org/10.1186/s13073-022-01094-yDOI Listing
August 2022

A Risk Stratification Model for Metastatic Lymph Nodes of Papillary Thyroid Cancer: A Retrospective Study Based on Sonographic Features.

Front Endocrinol (Lausanne) 2022 22;13:942569. Epub 2022 Jul 22.

Department of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Papillary thyroid carcinoma (PTC) has a high probability of cervical lymph node (LN) metastasis. The aim of the study was to develop an ultrasound risk stratification model to standardize the diagnosis of metastatic LNs of PTC.

Methods: Patients with suspicious thyroid nodules who underwent US examination and US guided fine-needle aspiration for cervical LNs were retrospectively collected. Univariate and multivariate logistic regression analyses were performed to assess the independent risk factor of metastatic LNs. According to the OR value of correlated indicators in logistic regression analysis, a risk stratification model was established.

Results: A total of 653 LNs were included. The independent risk factors of metastatic LNs were long-axis diameter/short-axis ≤ 2 (OR=1.644), absence of hilum (OR=1.894), hyperechogenicity (OR=5.375), calcifications (OR=6.201), cystic change (OR=71.818), and abnormal flow (OR=3.811) (P<0.05 for all). The risk stratification model and malignancy rate were as follows: 0-2 points, malignancy rate of 10.61%, low suspicion; 3-5 points, malignancy rate of 50.49%, intermediate suspicion, ≥6 points, malignancy rate of 84.81%, high suspicion. The area under the receiver operating characteristic curve for the model was 0.827 (95% CI 0.795-0.859).

Conclusions: Our established risk stratification model can effectively evaluate metastatic LNs in the patients with suspicious thyroid nodules, and it might provide a new strategy choice for clinical practice.
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http://dx.doi.org/10.3389/fendo.2022.942569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355421PMC
August 2022

Color change of glass ceramic restorations cemented by four types of dual-cured resin luting agents with different initiator systems.

Dent Mater J 2022 Aug 5. Epub 2022 Aug 5.

National Clinical Research Center for Oral Diseases & State Key Laboratory of Military Stomatology & Shaanxi Key Laboratory of Oral Diseases & Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University.

This study aimed to comprehensively evaluate the effect of dual-cured resin luting agents with different initiator systems on the color stability of glass ceramic restorations by simulating various clinical glass ceramic restorations. Three commonly used shades from each of the two dual-cured resin luting agents with an amine-initiation system or without it were studied. The individual specimens had different translucency and thickness and were artificially aged using a xenon light aging machine. The color was measured before and after aging using a digital spectrophotometer with the difference calculated and analyzed statistically. As results, the amine-free dual-cured resin luting agents were more color stable than those using amine-initiation systems for both uncovered and bonding groups. The translucency and thickness of the ceramic, and shade and type of the resin luting agent significantly affected the color stability of glass ceramic restorations.
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http://dx.doi.org/10.4012/dmj.2022-044DOI Listing
August 2022

A novel WRKY34-bZIP3 module regulates phenolic acid and tanshinone biosynthesis in Salvia miltiorrhiza.

Metab Eng 2022 Aug 4. Epub 2022 Aug 4.

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. Electronic address:

Phenolic acids and tanshinones are main bioactive compounds produced in Salvia miltiorrhiza widely used in treatment of cardiovascular diseases, which could be promoted by abscisic acid elicitation. However, the regulation mechanism remained to be elucidated. An ABA-inducible IIa WRKY transcription factor (TF) named SmWRKY34 exhibiting high homology with AtWRKY40 was isolated. SmWRKY34 exhibited a negative role on phenolic acids and tanshinones by directly regulating SmRAS and SmGGPPS. Moreover, ABA-responsive bZIP TF member named SmbZIP3 expressed significantly in SmWRKY34 transcriptome was screened. SmWRKY34 has a negative regulatory role on SmbZIP3. SmbZIP3 acts as a positive regulator in the biosynthesis of phenolic acids and tanshinones by targeting SmTAT and two tanshinone-promoting TFs SmERF128 and SmMYB9b. Taken together, we identified a new module WRKY34-bZIP3 involved in ABA signaling that manipulates phenolic acid and tanshinone accumulation, shedding new insights in metabolic engineering application in S. miltiorrhiza.
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http://dx.doi.org/10.1016/j.ymben.2022.08.002DOI Listing
August 2022

Thiourea with sulfur-donor as an effective additive for enhanced performance of lead-free double perovskite photovoltaic cells.

J Colloid Interface Sci 2022 Jul 29;628(Pt A):476-485. Epub 2022 Jul 29.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 210009, China; WA School of Mines: Minerals, Energy and Chemical Engineering (WASM-MECE), Curtin University, Perth, WA 6845, Australia. Electronic address:

Lead-free inorganic CsAgBiBr double perovskites have emerged as promising materials in perovskite solar cells (PSCs) to tackle the inferior stability and toxicity issues of organic-inorganic hybrid PSCs. However, the power conversion efficiencies (PCEs) of CsAgBiBr solar cells are remarkably restricted by the intrinsic and extrinsic defects of CsAgBiBr films. More specifically, the fast crystallization process in the formation of CsAgBiBr films strongly prevents the homogeneous growth of perovskite crystals, leading to inferior CsAgBiBr film quality. This work introduces a facile strategy to retard the crystallization of CsAgBiBr perovskites by introducing Lewis base additives into the precursor solution. The incorporation of a strongly coordinated thiourea additive with a sulfur donor leads to the generation of a Lewis acid base adduct, which retards the crystallization process for CsAgBiBr crystals, improves the quality of the CsAgBiBr film, decreases the defect density and inhibits charge carrier recombination. After optimization, the cell delivers a superior PCE of 3.07%, surpassing that reported for most CsAgBiBr-based solar cells in the literature, and exhibits outstanding stability with a PCE retention rate of 95% after 20 days of storage in air. This work provides an effective strategy for further improvements in the performance of inorganic lead-free CsAgBiBr-based photovoltaic cells.
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http://dx.doi.org/10.1016/j.jcis.2022.07.165DOI Listing
July 2022

Preoperative hypoalbuminemia in patients undergoing cardiac surgery: a meta-analysis.

Surg Today 2022 Aug 7. Epub 2022 Aug 7.

Department of Cardio-Thoracic Surgery, The Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, 330006, China.

The preoperative serum albumin level has been shown to be associated with adverse postoperative complications, meaning that hypoalbuminemia may also be a risk factor. We performed a meta-analysis to evaluate the association of serum albumin levels with survival and complication rates after cardiac surgery. Relevant articles were identified through seven databases. Twenty studies with 22553 patients (hypoalbuminemia group, n = 9903; normal group, n = 12650) who underwent cardiac surgery met the inclusion criteria after screening. The primary outcomes were that hypoalbuminemia was significantly correlated with serious long-term all-cause mortality (hazard ratio [HR]: 1.95 [1.54-2.48]; P < 0.00001) and increased mortality (risk ratio [RR] = 1.91 [1.61-2.27], P < 0.00001). Hypoalbuminemic patients with cardiopathy were more likely to suffer postoperative complications (bleeding, infections, renal injury, and others) than those whose serum albumin levels were normal. Furthermore, hypoalbuminemia increased the time in the intensive-care unit (ICU) (mean difference [MD] = 1.18 [0.49-1.87], P = 0.0008), length of hospital stay (LOS) (MD = 3.34, 95% CI: 1.88-4.80, P < 0.00001), and cardiopulmonary bypass time (CPB) (MD = 12.40 [1.13-23.66], P = 0.03). Hypoalbuminemia in patients undergoing cardiac surgery appears to have a poor all-cause mortality or increased risk of complications. Adjusted perioperative serum albumin levels and treatment strategies for this high-risk population have the potential to improve the survival.
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http://dx.doi.org/10.1007/s00595-022-02566-9DOI Listing
August 2022

Left iliac vein involvement is a protective factor against symptomatic pulmonary embolism in lower-left extremity deep vein thrombosis.

J Vasc Surg Venous Lymphat Disord 2022 Aug 2. Epub 2022 Aug 2.

Department of Vascular and Interventional Radiology, the second affiliated Hospital, Nanchang University, Nanchang 330006, China.

Objective: Left iliac vein compression is associated with left iliac vein thrombosis (IVT), potentially limiting the migration of the thrombus from this stenotic segment to the pulmonary arteries. We sought to investigate the differences in clinical characteristics and risk factors of symptomatic pulmonary embolism (SPE) in patients with deep vein thrombosis (DVT) in different limbs and anatomical locations.

Methods: A retrospective analysis was conducted in 1476 patients with acute unilateral lower extremity DVT. Differences of clinical characteristics and risk factors between left-side and right-side DVT, IVT and non-IVT, cases with SPE and cases without SPE were compared. Risk factors for SPE were investigated using logistic regression analysis.

Results: SPE was more common in right-side DVT patients than left-side DVT patients (13.8% vs 7.0%, p<0.001). SPE incidence in left IVT, 5.4%, was lower than that in left non-IVT, right IVT and right non-IVT (12.8%, 10.1%, 16.6%, p<0.001). There was no difference in SPE incidence among patients with left non-IVT, right IVT and right non-IVT (p>0.05). In left-side DVT patients, male sex was associated with increased odds of SPE (OR: 1.77, 95% CI: 1.10-2.85, P=0.02). IVT, surgery and immobilization were associated with decreased odds of SPE (OR:0.46, 95% CI: 0.28-0.76, p<0.01; OR: 0.55, 95% CI:0.32-0.95, p=0.03; OR: 0.53, 95% CI:0.32-0.86, p=0.01). In right-side DVT patients, provoked DVT was associated with decreased odds of SPE (OR:0.50, 95% CI: 0.27-0.93, p=0.03).

Conclusion: Left IVT is associated with a lower SPE incidence than right-side DVT. Left IVT and may be a relative protective factor against SPE.
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http://dx.doi.org/10.1016/j.jvsv.2022.05.015DOI Listing
August 2022

A multi-layer functional genomic analysis to understand noncoding genetic variation in lipids.

Am J Hum Genet 2022 08;109(8):1366-1387

Division of Genome Science, Department of Precision Medicine, National Institute of Health, Chungbuk, South Korea.

A major challenge of genome-wide association studies (GWASs) is to translate phenotypic associations into biological insights. Here, we integrate a large GWAS on blood lipids involving 1.6 million individuals from five ancestries with a wide array of functional genomic datasets to discover regulatory mechanisms underlying lipid associations. We first prioritize lipid-associated genes with expression quantitative trait locus (eQTL) colocalizations and then add chromatin interaction data to narrow the search for functional genes. Polygenic enrichment analysis across 697 annotations from a host of tissues and cell types confirms the central role of the liver in lipid levels and highlights the selective enrichment of adipose-specific chromatin marks in high-density lipoprotein cholesterol and triglycerides. Overlapping transcription factor (TF) binding sites with lipid-associated loci identifies TFs relevant in lipid biology. In addition, we present an integrative framework to prioritize causal variants at GWAS loci, producing a comprehensive list of candidate causal genes and variants with multiple layers of functional evidence. We highlight two of the prioritized genes, CREBRF and RRBP1, which show convergent evidence across functional datasets supporting their roles in lipid biology.
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http://dx.doi.org/10.1016/j.ajhg.2022.06.012DOI Listing
August 2022

Recent Advances in TiO2-based Photoanodes for Photoelectrochemical Water Splitting.

Chem Asian J 2022 Aug 4. Epub 2022 Aug 4.

Shanghai Aerospace Hydrogen Energy Technology Co. Ltd, Department of R & D, CHINA.

Photoelectrochemical (PEC) water splitting has attracted a great attention in the past several decades which holds great promise to address global energy and environmental issues by converting solar energy into hydrogen. However, its low solar-to-hydrogen (STH) conversion efficiency remains a bottleneck for practical application. Developing efficient photoelectrocatalysts with high stability and high STH conversion efficiency is one of the key challenges. As a typical n-type semiconductor, titanium dioxide (TiO 2 ) exhibits high PEC water splitting performance, especially high chemical and photo stability. But, TiO 2 has also disadvantages such as wide band gap and fast electron-hole recombination rate, which seriously hinder its PEC performance. This review focuses on recent development in TiO 2 -based photoanodes as well as some key fundamentals. The corresponding mechanisms and key factors for high STH, and controllable synthesis and modification strategies are highlighted in this review. We conclude finally with an outlook providing a critical perspective on future trends on TiO 2 -based photoanodes for PEC water splitting.
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http://dx.doi.org/10.1002/asia.202200668DOI Listing
August 2022

Porous organic polymer overcomes the post-treatment phototoxicity of photodynamic agents and maintains their antitumor efficiency.

Acta Biomater 2022 Jul 30. Epub 2022 Jul 30.

Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China. Electronic address:

Since 1995, photodynamic therapy (PDT) has been utilized as an effective method for cancer treatment. However, the residues of photosensitizers in the normal tissues after PDT can be activated by sunlight to cause severe skin phototoxicity, for which currently there are no clinical solutions. As a result, post-PDT patients need to remain out of sunlight for up to five weeks, which produces great living and mental burdens for patients. Herein, we report that a biocompatible porous organic polymer (POP) with average 3.1 nm porosity is able to suppress the skin phototoxicity of clinically used porphyrin-based photodynamic agents (PDAs), including Photofrin, Talaporfin and Hiporfin, through an adsorption-elimination mechanism. Fluorescence titration and dialysis experiments show that POP can adsorb and retain the PDAs at a micromolar concentration. In vivo experiments demonstrate that POP can significantly suppress the skin phototoxicity caused by all the three PDAs without reducing their PDT efficacy. STATEMENT OF SIGNIFICANCE: Up to now, no efficient clinical treatment for the inhibition of post-PDT phototoxicity of clinically used porphyrin-based PDAs is available. In the manuscript, a water-soluble cationic porous organic polymer has been revealed to include three clinically used PDAs. In vivo experiments show that this inclusion remarkably reduces the content of PDAs in mouse skins, leading to significant alleviation of their post-PDT phototoxicity without no negative effect on their PDT efficacy. Thus, this work provides a strategy for overcoming the drawback of clinically used photodynamic agents.
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http://dx.doi.org/10.1016/j.actbio.2022.07.043DOI Listing
July 2022

Context-dependent tumor-suppressive BMP signaling in diffuse intrinsic pontine glioma regulates stemness through epigenetic regulation of CXXC5.

Nat Cancer 2022 Aug 1. Epub 2022 Aug 1.

MOE Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University, Beijing, China.

The most lethal subtype of diffuse intrinsic pontine glioma (DIPG) is H3K27M. Although ACVR1 mutations have been implicated in the pathogenesis of this currently incurable disease, the impacts of bone morphogenetic protein (BMP) signaling on more than 60% of H3K27M DIPG carrying ACVR1 wild-type remain unknown. Here we show that BMP ligands exert potent tumor-suppressive effects against H3.3K27M and ACVR1 WT DIPG in a SMAD-dependent manner. Specifically, clinical data revealed that many DIPG tumors have exploited the capacity of CHRDL1 to hijack BMP ligands. We discovered that activation of BMP signaling promotes the exit of DIPG tumor cells from 'prolonged stem-cell-like' state to differentiation by epigenetically regulating CXXC5, which acts as a tumor suppressor and positive regulator of BMP signaling. Beyond showing how BMP signaling impacts DIPG, our study also identified the potent antitumor efficacy of Dacinostat for DIPG. Thus, our study delineates context-dependent features of the BMP signaling pathway in a DIPG subtype.
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http://dx.doi.org/10.1038/s43018-022-00408-8DOI Listing
August 2022

Intelligent pH-sensing film based on polyvinyl alcohol/cellulose nanocrystal with purple cabbage anthocyanins for visually monitoring shrimp freshness.

Int J Biol Macromol 2022 Jul 27;218:900-908. Epub 2022 Jul 27.

Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524001, China.

We aimed to prepare a new pH-sensing film based on the immobilization of purple cabbage anthocyanins (PCA) into Polyvinyl alcohol (PVA) reinforced by cellulose nanocrystals (CNC). FT-IR, XRD and TGA were used to assess the intermolecular interactions and thermo-stability of films. The addition of CNC and PCA resulted in an enhancement in UV-vis barrier, mechanical properties and moisture resistance. Inclusion of PCA imparted intelligent properties to the films. PCA-loaded films displayed strong visually detectable colorimetric responses to pH (2-13) and volatile ammonia. When applied to monitor shrimp freshness at 4 °C, PVA/CNC films containing 0.6 % PCA exhibited conspicuous color fluctuations from purple to gray blue upon deterioration. As a result, PVA/CNC-PCA colorimetric films were considered as intelligent packaging labels with significant mechanical, water vapor barrier properties and pH-sensing qualities for visual quality evaluation of fresh seafood products.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.194DOI Listing
July 2022

Electrochemical Upgrading of Formic Acid to Formamide via Coupling Nitrite Co-Reduction.

J Am Chem Soc 2022 Jul 29. Epub 2022 Jul 29.

Institute of Molecular Plus, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.

Formic acid (HCOOH) can be exclusively prepared through CO electroreduction at an industrial current density (0.5 A cm). However, the global annual demand for formic acid is only ∼1 million tons, far less than the current CO emission scale. The exploration of an economical and green approach to upgrading CO-derived formic acid is significant. Here, we report an electrochemical process to convert formic acid and nitrite into high-valued formamide over a copper catalyst under ambient conditions, which offers the selectivity from formic acid to formamide up to 90.0%. Isotope-labeled in situ attenuated total reflection surface-enhanced infrared absorption spectroscopy and quasi in situ electron paramagnetic resonance results reveal the key C-N bond formation through coupling *CHO and *NH intermediates. This work offers an electrochemical strategy to upgrade CO-derived formic acid into high-value formamide.
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http://dx.doi.org/10.1021/jacs.2c05660DOI Listing
July 2022

Functional Studies and Revision of the NFAT-133/TM-123 Biosynthetic Pathway in .

ACS Chem Biol 2022 Jul 29. Epub 2022 Jul 29.

Department of Pharmaceutical Sciences, Oregon State University, Corvallis, Oregon 97331-3507, United States.

The biosynthetic gene cluster of NFAT-133, an inhibitor of the nuclear factor of activated T cells, was recently identified in ATCC 27456. This cluster is conspicuous by its highly disordered noncollinear type I modular polyketide synthase (PKS) genes that encode PKSs with one module more than those expected for the heptaketide NFAT-133 biosynthesis. Thus, the major metabolite NFAT-133 was proposed to derive from an octaketide analogue, TM-123. Here, we report that further bioinformatic analysis and gene inactivation studies suggest that NFAT-133 is not derived from TM-123 but rather a product of programmed KS extension skipping of a nascent heptaketide from the PKS assembly line that produces TM-123. Furthermore, identification of NFAT-133/TM-123 analogues from mutants of the ATCC 27456 strain suggests that NftN (a putative dehydrogenase), NftE (a cytochrome P450), and NftG (a putative hydrolase/decarboxylase) function "" during the polyketide chain assembly processes.
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http://dx.doi.org/10.1021/acschembio.2c00454DOI Listing
July 2022

Increased Mortality Trends in Patients With Chronic Non-communicable Diseases and Comorbid Hypertension in the United States, 2000-2019.

Front Public Health 2022 11;10:753861. Epub 2022 Jul 11.

Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, China.

Background: According to the Sustainable Development Goals (SDGs), countries are required to reduce the mortality rates of four main non-communicable diseases (NCDs), including cardiovascular diseases (CVDs), diabetes mellitus (DM), chronic respiratory diseases (CRDs), and cancer (CA), by one-third in 2030 from the 2015 level. However, progress fell short of expectations, partly attributed to the high rates of hypertension-related NCD mortality. This study aimed to investigate the mortality trends of SDG-targeted NCDs with comorbid hypertension. In addition, the disparities in mortality rates among different demographic subgroups were further explored.

Methods: Mortality data from 2000 to 2019 were acquired from the Centers for Disease Control and Prevention in the United States. SDG-targeted NCDs were considered the underlying causes of death, and hypertension was considered a multiple cause of death. Permutation tests were performed to determine the time points of Joinpoints for mortality trends. The annual percent changes and average annual percent changes (AAPCs), as well as 95% confidence intervals (CIs), were calculated to demonstrate the temporary trend of mortality rates overall and by age, sex, ethnicity, and region.

Results: The hypertension-related DM, CRD, and CA mortality rates increased over the 20 years, of which the AAPCs were 2.0% (95% CI: 1.4%, 2.6%), 3.2% (95% CI: 2.8%, 3.6%), and 2.1% (95% CI: 1.6%, 2.6%), respectively. Moreover, despite decreasing between 2005 and 2015, the hypertension-related CVD mortality rate increased from 2015 to 2019 [APC: 1.3% (95% CI: 0.7%, 1.9%)]. The increased trends were consistent across most age groups. Mortality rates among men were higher and increased faster than those among women. The hypertension-related CVD, DM, and CA mortality rates among African American people were higher than those among White people. The increased mortality rates in rural areas, especially in rural south, were higher than those in urban areas.

Conclusion: In the United States, the hypertension-related DM, CRD, and CA mortality rates increased between 2000 and 2019, as well as hypertension-related CVD mortality between 2015 and 2019. Disparities existed among different sexes, ethnicities, and areas. Actions to prevent and manage hypertension among patients with NCDs are required to reduce the high mortality rates and minimize disparities.
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http://dx.doi.org/10.3389/fpubh.2022.753861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309719PMC
July 2022

An efficient photo Fenton system for in-situ evolution of HOvia defective iron-based metal organic [email protected] core-shell Z-scheme heterojunction nanoreactor.

J Hazard Mater 2022 Sep 21;437:129436. Epub 2022 Jun 21.

Department of Environmental Science, School of Chemistry and Materials Science, Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin 150080, PR China; Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China. Electronic address:

The fabrication of an efficient photoFenton system without the addition of HO is still a challenge and is cost-effective and favorable for practical applications. In this work, a [email protected] Z-scheme heterojunction nanoreactor was successfully fabricated, in which hierarchical two-dimensional (2D) ZnInS nanosheets are coated on defective iron-based metal-organic frameworks (MOFs) (NH-MIL-88B(Fe)), realizing efficient in-situ evolution of HO and constructing an optimal heterogeneous Fenton platform. The degradation rates of defective NH-MIL-88B(Fe)@ZnInS (0.4 g L) for bisphenol A and ofloxacin under visible light irradiation within 180 min reached 99.4% and 98.5%, respectively, and the photocatalytic hydrogen production efficiency was approximately 502 μmol h g. The excellent photoFenton performance was attributed to the introduction of ligand defects into the MOF, which can adjust the band structure to enhance the light absorption capacity, and the in-situ generation of HO accelerating the Fe/Fe conversion. In addition, the formation of the [email protected] nanoreactor Z-scheme heterojunction structure promoted spatial charge separation. This strategy offers new ideas for constructing efficient photocatalysis and photoFenton systems.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129436DOI Listing
September 2022

Association of marital status with cognitive function in Chinese hypertensive patients: a cross-sectional study.

BMC Psychiatry 2022 07 27;22(1):504. Epub 2022 Jul 27.

Department of Cardiovascular, the Second Affiliated Hospital of Nanchang University, No. 1 Minde Road, Nanchang, 330006, Jiangxi, China.

Purpose: The aim of this study was to evaluate the association of marital status with cognitive function and to examine the potential effect modifiers in Chinese hypertensive populations.

Methods: A total of 9,525 adult Chinese hypertensive patients were enrolled in this cross-sectional study. Cognitive function, as the dependent variable in our study, was assessed by the Chinese version of the Mini-Mental State Examination (MMSE). We adjusted for potential confounding factors in multiple linear regression models to examine the relationship of marital status with cognitive function. In addition, we divided the population according to sex to explore whether there were sex-specific differences.

Results: Among the 9,525 study participants, the mean (SD) age for men was 63.5 (10.3) years, and the mean MMSE score was 24.9 ± 5.0, whereas for women, the mean (SD) age was 63.8 (9.3) years, and the mean MMSE score was 19.4 ± 6.4. Unmarried persons had lower scores on the MMSE and lower subscores in each of the cognitive domains. A stronger correlation between marital status and a lower MMSE score was statistically significant in men (unmarried men: β = -1.55; 95% CI: -1.89, -1.21) but not women (unmarried women: β = -0.22; 95% CI: -0.56, 0.12; p interaction = 0.006). Compared to men who were widowed or divorced, never married men were more likely to have lower MMSE scores (β = -2.30, 95% CI -3.10,-1.50; p < 0.001).

Conclusions: Our study demonstrated that being unmarried is an extremely important but neglected social risk factor for cognitive function. Sex was a strong effect modifier: being unmarried was correlated with a higher risk of cognitive decline than being married in Chinese hypertensive men, especially among older men, but this correlation was not observed among women. Moreover, never married men showed poorer cognitive function than those who were divorced or widowed.
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http://dx.doi.org/10.1186/s12888-022-04159-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327272PMC
July 2022

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of (Mill.) I.M. Johnst, a Multipurpose Woody Plant.

Plants (Basel) 2022 Jul 26;11(15). Epub 2022 Jul 26.

Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou 510642, China.

(Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments ( < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.
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http://dx.doi.org/10.3390/plants11151937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332613PMC
July 2022

LINC00265 Promotes Metastasis and Progression of Hepatocellular Carcinoma by Interacting with E2F1 at The Promoter of .

Cell J 2022 Jun 29;24(6):294-301. Epub 2022 Jun 29.

Department of Psychiatry, Guangxi Zhuang Autonomous Region Brain Hospital, Liuzhou, Guangxi, China. Email:

Objective: This study aimed to explore biological function of long intergenic non-protein coding RNA 265 (LINC00265) in hepatocellular carcinoma (HCC) cells, and evaluate its potential function as a biomarker.

Materials And Methods: In this experimental study, GEPIA database and Kaplan-Meier Plotter database were employed to analyze LINC00265 expression in HCC tissue samples and its predicting value for prognosis. LINC00265 expression in HCC tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). After overexpressing and knocking-down of LINC00265 in HCC cells, cell counting kit-8 (CCK-8) and 5-Ethynyl-2'- deoxyuridine (EdU) assays were adopted to detect proliferation of HCC cells. Transwell assay was used to detect migration and invasion of HCC cells. Interaction of LINC00265 with E2F transcription factor 1 (E2F1) was verified by the catRAPID online analysis tool, RNA pull-down experiment and RNA binding protein immunoprecipitation (RIP) assay. Binding of E2F1 to the promoter region of cyclin-dependent kinases 2 () was detected by dual-luciferase reporter assay and chromatin immunoprecipitation. Regulatory effects of LINC00265 and E2F1 on expression were probed by Western blot.

Results: LINC00265 expression was increased in HCC tissues and cells. LINC00265 overexpression promoted proliferation, migration and invasion of HCC cells, and knocking-down LINC00265 worked oppositely. LINC00265 could bind to E2F1 and it could enhance combination of E2F1 and promoter regions, thus promoting CDK2 transcription. LINC00265 overexpression promoted expression of in HCC cells.

Conclusion: Our data suggested that LINC00265 can promote malignant behaviors of HCC cells by recruiting E2F1 to the promoter region of .
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http://dx.doi.org/10.22074/cellj.2022.8035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315211PMC
June 2022

Electric field and strain engineering tuning Rashba spin splitting in quasi-one-dimensional organic-inorganic hybrid perovskites (MV)AICl (MV = methylviologen, A = Bi, Sb).

Phys Chem Chem Phys 2022 Aug 3;24(30):18401-18407. Epub 2022 Aug 3.

Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, School of Science, Tianjin University, Tianjin 300072, China.

We systematically study the Rashba spin texture of lead-free quasi-one-dimensional organic-inorganic hybrid perovskites (OIHP), (MV)AICl (MV = methylviologen, A = Bi, Sb) with first-principles calculations. The - plane Rashba spin splitting was found to depend on the composition of Bi (Sb) and I atoms at band edges. Importantly, increasing ferroelectric polarization and the stretch along the -direction can effectively enhance the amplitude of the Rashba spin splitting. This work provides an avenue for electric field and strain-controlled spin splitting and highlights the potential of quasi-one-dimensional OIHP for further applications in spin field effect transistors and photovoltaic cells.
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http://dx.doi.org/10.1039/d2cp02059aDOI Listing
August 2022

A new megastigmane glycoside, a new organic acid glycoside and other constituents with anticomplementary activity from .

Nat Prod Res 2022 Jul 25:1-6. Epub 2022 Jul 25.

Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Yanbian University, Yanji, China.

A new megastigmane glycoside, (1,5,6,7)-megastigman-3,9-dione-7-en-6,11-diol 11---D-glucopyranoside (), and a new organic acid glycoside, methyl (4 )-4---D-glucopyranosyl-decanoate (), together with eight known compounds (-), were isolated from the aerial parts of Turcz. ex Bess. (Asteraceae). Their chemical structures were elucidated by 1 D and 2 D NMR and HR-ESI-MS spectra and DP4+ probability analysis. Among the identified compounds, compounds , and were isolated from the family Asteraceae, and compounds , and - were identified from the genus for the first time. All of the compounds were evaluated for their anticomplementary activity against the classical pathway (CP) and the alternative pathway (AP). Compounds and showed anticomplementary activity with the CH values of 0.31 ± 0.08 and 0.50 ± 0.09 mM, respectively.
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http://dx.doi.org/10.1080/14786419.2022.2104273DOI Listing
July 2022

Incorporating family disease history and controlling case-control imbalance for population based genetic association studies.

Bioinformatics 2022 Jul 25. Epub 2022 Jul 25.

Graduate School of Data Science, Seoul National University, Seoul, Korea.

Background: In the genome-wide association analysis of population-based biobanks, most diseases have low prevalence, which results in low detection power. One approach to tackle the problem is using family disease history, yet existing methods are unable to address type I error inflation induced by increased correlation of phenotypes among closely related samples, as well as unbalanced phenotypic distribution.

Results: We propose a new method for genetic association test with family disease history, TAPE (mixed-model-based Test with Adjusted Phenotype and Empirical saddlepoint approximation), which controls for increased phenotype correlation by adopting a two-variance-component mixed model, accounts for case-control imbalance by using empirical saddlepoint approximation, and is flexible to incorporate any existing adjusted phenotypes such as phenotypes from the LT-FH method. We show through simulation studies and analysis of UK-Biobank data of white British samples and the Korean Genome and Epidemiology Study (KoGES) of Korean samples that the proposed method is robust and yields better calibration compared to existing methods while gaining power for detection of variant-phenotype associations.

Availability: The summary statistics and code generated in this study are available at https://github.com/styvon/TAPE.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btac459DOI Listing
July 2022

Subtyping of metastatic breast cancer based on plasma circulating tumor DNA alterations: An observational, multicentre platform study.

EClinicalMedicine 2022 Sep 18;51:101567. Epub 2022 Jul 18.

Department of Breast Cancer Medical Oncology, Hunan Cancer Hospital, Changsha, 410013, China.

Background: After multiple lines of therapies, no guideline or consensus is currently available for the treatment of patients with metastatic breast cancer. This study aims to evaluate the efficacy of a novel re-subtyping and treatment strategy based on ctDNA alterations.

Methods: This observational, multicentre study recruited 223 patients with metastatic breast cancer intending to receive late-line therapy from Dec 1, 2016, to June 31, 2019. This study took place in Hunan Cancer Hospital, the Forth Hospital of Changsha and Zhuzhou Central Hospital in China. ctDNA alterations were assessed by next-generation sequencing (NGS). Patients with druggable ctDNA alterations were treated with corresponding targeted drugs which are clinically available. Other patients received physician-chosen treatment. This study was registered with ClinicalTrials.gov, NCT05079074.

Findings: The progression-free survival (hazard ratio: 0.45, 95% Confidence Interval (CI): 0.33-0.62,  < 0.0001) and disease control rate (89.4% 65.9%,  < 0.0001) were significantly improved in patients who received druggable ctDNA alteration-guided therapy compared with those of patients who received physician-chosen treatment. ctDNA alterations with top rank and high clustering scores were classified into four subtypes based on their functions as follows: 1) extracellular function (ECF), 2) cell proliferation (CP), 3) nuclear function (NF), and 4) cascade signaling pathway (CSP). A significant benefit from ctDNA alteration-guided treatment was observed in patients with NF and CSP ctDNA alterations, with hazard ratios of 0.39 (95% CI: 0.24-0.65,  = 0.0003) and 0.14 (95% CI: 0.04-0.46,  < 0.0001), respectively.

Interpretation: After multiline traditional pathological HR/HER2 subtype-guided therapies, ctDNA testing could identify druggable ctDNA alterations to guide late-line therapy for patients with metastatic breast cancer.

Funding: This work was supported by Key Grants of Research and Development in Hunan Province (2018SK2124, 2018SK2120), Natural Science Foundation of Hunan (2019JJ50360), Hunan Provincial Health Commission Project (B2019085, B2019089 and C2019070), and Changsha Science and Technology Project (kq2004125 and kq2004137).
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http://dx.doi.org/10.1016/j.eclinm.2022.101567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304912PMC
September 2022

PD-1 Inhibitor Combined With Radiotherapy and GM-CSF (PRaG) in Patients With Metastatic Solid Tumors: An Open-Label Phase II Study.

Front Immunol 2022 8;13:952066. Epub 2022 Jul 8.

Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Patients with metastatic cancer refractory to standard systemic therapies have a poor prognosis and few therapeutic options. Radiotherapy can shape the tumor microenvironment (TME) by inducing immunogenic cell death and promoting tumor recognition by natural killer cells and T lymphocytes. Granulocyte macrophage-colony stimulating factor (GM-CSF) was known to promote dendric cell maturation and function, and might also induce the macrophage polarization with anti-tumor capabilities. A phase II trial (ChiCTR1900026175) was conducted to assess the clinical efficacy and safety of radiotherapy, PD-1 inhibitor and GM-CSF (PRaG regimen). This trial was registered at http://www.chictr.org.cn/index.aspx. A PRaG cycle consisted of 3 fractions of 5 or 8 Gy delivered for one metastatic lesion from day 1, followed by 200 μg subcutaneous injection of GM-CSF once daily for 2 weeks, and intravenous infusion of PD-1 inhibitor once within one week after completion of radiotherapy. The PRaG regimen was repeated every 21 days for at least two cycles. Once the PRaG therapy was completed, the patient continued PD-1 inhibitor monotherapy until confirmed disease progression or unacceptable toxicity. The primary endpoint was objective response rate (ORR). A total of 54 patients were enrolled with a median follow-up time of 16.4 months. The ORR was 16.7%, and the disease control rate was 46.3% in intent-to-treat patients. Median progression-free survival was 4.0 months (95% confidence interval [CI], 3.3 to 4.8), and median overall survival was 10.5 months (95% CI, 8.7 to 12.2). Grade 3 treatment-related adverse events occurred in five patients (10.0%) and grade 4 in one patient (2.0%). Therefore, the PRaG regimen was well tolerated with acceptable toxicity and may represent a promising salvage treatment for patients with chemotherapy-refractory solid tumors. It is likely that PRaG acts heating upthe TME with radiotherapy and GM-CSF, which was further boosted by PD-1 inhibitors.
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http://dx.doi.org/10.3389/fimmu.2022.952066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304897PMC
July 2022

Ginsenoside Rb1 Ameliorated Bavachin-Induced Renal Fibrosis via Suppressing Bip/eIF2α/CHOP Signaling-Mediated EMT.

Front Pharmacol 2022 8;13:872474. Epub 2022 Jul 8.

Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing, China.

The nephrotoxicity of , an effective traditional Chinese medicine for vitiligo treatment, has been reported. As one of the main toxic components in , bavachin (BV) was considered to be related to -caused adverse outcomes, but the direct evidence and molecular mechanism underlying BV-induced nephrotoxicity are not well elucidated. Therefore, this study was designed to confirm whether BV would cause toxic effects on the kidney and explore the possible mode of action. Our results demonstrated that days' treatment with 0.5 μM BV indeed caused obvious renal fibrosis in the zebrafish kidney. The obvious E- to N-cadherin switch and the expressions of proteins promoting epithelial-mesenchymal transition (EMT) were observed in BV-treated human renal tubular epithelial and zebrafish kidneys. In addition, elevated reactive oxygen species (ROS) levels and Bip/eIF2α/CHOP-mediated endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) were caused by BV, both of which could be reversed by ROS scavenger N-acetyl-L-cysteine (NAC). Also, blocking ER stress-caused cytoplasmic Ca overload with 4-PBA notably alleviated BV-induced alterations in key molecular events related to EMT and renal fibrosis. Furthermore, of the natural compounds subjected to screening, ginsenoside Rb1 significantly downregulated BV-induced ER stress by inhibiting ROS generation and following the activation of Bip/eIF2α/CHOP signaling in HK2 cells. Subsequently, BV-triggered EMT and renal fibrosis were both ameliorated by ginsenoside Rb1. In summary, our findings suggested that BV-induced ROS promoted the appearance of EMT and renal fibrosis mainly via Bip/eIF2α/CHOP-mediated ER stress. This ER stress-related toxic pathway might be a potential intervention target for BV-caused renal fibrosis, and ginsenoside Rb1 would be a promising drug against BV- or -induced nephrotoxicity.
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http://dx.doi.org/10.3389/fphar.2022.872474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304982PMC
July 2022

Modeling the Uncertainty of Information Propagation for Rumor Detection: A Neuro-Fuzzy Approach.

IEEE Trans Neural Netw Learn Syst 2022 Jul 22;PP. Epub 2022 Jul 22.

Automatic rumor detection is critical for maintaining a healthy social media environment. The mainstream methods generally learn rich features from information cascades by modeling the cascade as a tree or graph structure where edges are built based on interactions between a tweet and retweets. Some psychology studies have empirically shown that users' various subjective factors always cause the uncertainty of interactions such as differences among interactive behavior activation thresholds or semantic relevancy. However, previous works model interactions by employing a simple fully connected layer on fixed edge weights in the graph and cannot reasonably describe this inherent uncertainty of complex interactions. In this article, inspired by the fuzzy theory, we propose a novel neuro-fuzzy method, fuzzy graph convolutional networks (FGCNs), to sufficiently understand uncertain interactions in the information cascade in a fuzzy perspective. Specifically, a new strategy of graph construction is first designed to convert each information cascade into a heterogeneous graph structure with the consideration of explicit interactive behaviors between a tweet and its retweet, as well as implicit interactive behaviors among retweets, enriching more structural clues in the graph. Then, we improve graph convolutional networks by incorporating edge fuzzification (EF) modules. The EFs adapt edge weights according to predefined membership to enhance message passing in the graph. The proposed model can provide a stronger relational inductive bias for expressing uncertain interactions and capture more discriminative and robust structural features for rumor detection. Extensive experiments demonstrate the effectiveness and superiority of FGCN on both rumor detection and early rumor detection.
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http://dx.doi.org/10.1109/TNNLS.2022.3190348DOI Listing
July 2022

Ambient synthesis of an iminium-linked covalent organic framework for synergetic RNA interference and metabolic therapy of fibrosarcoma.

Chem Sci 2022 Jul 15;13(26):7846-7854. Epub 2022 Jun 15.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University Jinan 250014 China

Small interfering RNA (siRNA)-mediated gene silencing is a promising therapeutic approach. Herein, we report the ambient synthesis of a positively charged iminium-linked covalent organic framework by a three-component one-pot reaction. Through anion exchange and siRNA adsorption, the resulting multifunctional [email protected], which possesses both the HK2 inhibitor 3-bromopyruvate and SLC7A11 siRNA, exhibits powerful synergistic antitumor activity against fibrosarcoma the ferroptosis and apoptosis pathways.
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http://dx.doi.org/10.1039/d2sc02297dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258322PMC
July 2022

Neutrophil-lymphocyte ratio as a potential marker for differential diagnosis between spinal tuberculosis and pyogenic spinal infection.

J Orthop Surg Res 2022 Jul 21;17(1):357. Epub 2022 Jul 21.

Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.

Objective: Distinguishing spinal tuberculosis and pyogenic spinal infection is extremely important. The neutrophil-lymphocyte ratio (NLR), a simple indicator, has been shown to be a novel inflammatory marker. The objective of our study was to determine whether the NLR could be a potential indicator for discriminating spinal tuberculosis (STB) from pyogenic spinal infection (PSI).

Methods: We compared the clinical and laboratory characteristics of 146 patients diagnosed with STB and 60 participants with PSI from the First Affiliated Hospital of Nanjing Medical University. The NLR's diagnostic ability for differential diagnosis was assessed and compared to other hematological indicators, including the platelet-lymphocyte ratio (PLR).

Results: The NLR in STB patients was considerably lower than that in PSI patients [3.85 (2.70-5.71) vs. 10.82 (6.79-17.62), P < 0.001]. An NLR of 6.742 was proposed as an optimal cutoff value for distinguishing patients with STB from those with PSI (sensitivity 78.33%, specificity 83.56%). However, the NLR's area under the curve [0.87, 95% confidence interval (CI) 0.81-0.92] was considerably higher than that of the PLR (0.73, 95% CI 0.65-0.80; P < 0.0001).

Conclusion: NLR levels could be a valuable laboratory diagnostic for distinguishing patients with STB from those who have PSI.
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http://dx.doi.org/10.1186/s13018-022-03250-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301616PMC
July 2022
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