Publications by authors named "Wei Zhang"

15,284 Publications

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Versatile Gold-Coupled Te-Carbon Dots for Quantitative Monitoring and Efficient Scavenging of Superoxide Anions.

Anal Chem 2021 Jun 22. Epub 2021 Jun 22.

College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014 P. R. China.

The superoxide anion (O) is a reactive oxygen species (ROS) that functions as an important regulator of signal transduction in living systems. However, excess O can cause metabolic imbalances and oxidative damage inside cells. Quantitative detection and efficient scavenging of O are therefore critical for maintaining intracellular redox balance and homeostasis. In this work, a nanomaterial (Au-TeCD) composed of BSA-modified gold nanoparticles (AuNPs) complexed with tellurium-containing carbon dots (TeCDs) was constructed. The introduction of Au-TeCDs to solutions containing superoxide resulted in enhanced elimination of the anion, indicating that Au-TeCDs are able to scavenge O from the surrounding environment. Notably, the respective TeCD and AuNP components of the Au-TeCDs were found to emit fluorescence at 425 and 640 nm upon exposure to superoxide anions. This unique spectroscopic property of Au-TeCDs allowed levels of O in solution to be quantified using dual-fluorescence detection. The Au-TeCDs developed herein also exhibited low-cytotoxicity, versatile capabilities for fluorescence imaging, and effective scavenging of O in living cells. Taken together, these results suggest that Au-TeCDs act as effective tools for monitoring superoxide concentrations in complex mixtures and may be developed as possible therapeutics designed to scavenge excess ROS from diseased cells.
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http://dx.doi.org/10.1021/acs.analchem.1c00844DOI Listing
June 2021

Identification of a phage-derived depolymerase specific for KL64 capsule of Klebsiella pneumoniae and its anti-biofilm effect.

Virus Genes 2021 Jun 22. Epub 2021 Jun 22.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

The increasing prevalence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a serious threat to global health. Phages and phage-derived enzymes gained increasing attention for controling CRKP infections. In this study, a lytic phage P510 infecting KL64 type K. pneumoniae was isolated and characterized. Whole genome analysis and electron microscopy analysis showed that phage P510 belonged to genus Przondovirus, family Autographiviridae, the order Caudovirales. The tail fiber protein of the phage was predicted to encode capsule depolymerase. Further analysis demonstrated that recombinant depolymerase P510dep had polysaccharide-degrading activity against KL64-types capsule of K. pneumoniae, and its lysis spectrum matched to host range of phage P510. We also demonstrated that the recombinant depolymerase was able to significantly inhibit biofilm formation. The discovery of the phage-derived depolymerase lays the foundation for controlling the spread of CRKPs.
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http://dx.doi.org/10.1007/s11262-021-01847-8DOI Listing
June 2021

Transfer, Amplification, Storage and Complete Self-Recovery of Supramolecular Chirality in Achiral Polymer System.

Angew Chem Int Ed Engl 2021 Jun 22. Epub 2021 Jun 22.

Soochow University, College of Chemistry, Chemical Engineering and Materials Science, CHINA.

Supramolecular chirality and its complete self-recovery ability are highly mystical in nature and biological systems, which remains a major challenge today. Herein, we firstly demonstrate that the partially cross-linked azobenzene (Azo) units can be employed as the potential chiral trigger to fully heal the destroyed helical superstructure in achiral nematic polymer system. Combining the self-assembly of Azo units and terminal hydroxyl in polymer side chains allows vapor-induced chiral nematic phase and covalent fixation of the superstructure via acetal reaction. The induced helical structure of Azo units can be stored by the inter-chain cross-linking, even after the removal of chiral source. Most interestingly, the stored chiral information can trigger the perfect chiral self-recovery (CSR) behaviours after being destroyed by UV light, heat and solvent. The results pave a new way for producing novel chiroptical materials with reversible chirality from achiral sources.
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http://dx.doi.org/10.1002/anie.202107992DOI Listing
June 2021

Effectiveness of an individually tailored home-based exercise rogramme for pre-frail older adults, driven by a tablet application and mobility monitoring: a pilot study.

Eur Rev Aging Phys Act 2021 Jun 21;18(1):10. Epub 2021 Jun 21.

Department of Orthopaedics, University of Groningen, University Medical Center Groningen, P.O. BOX 30.001, 9700 RB, Groningen, The Netherlands.

Objectives: To gain first insight into the effectiveness of a home-based exercise programme for pre-frail older adults with independent use of novel ICT technology.

Methods: A pilot study. Forty pre-frail older adults joined a six-month home-based exercise programme using a tablet PC for exercise administration and feedback, and a necklace-worn motion sensor for daily physical activity registration. Participants received weekly telephone supervision during the first 3 months and exercised independently without supervision from a coach during the last 3 months. Functional performance and daily physical activity were assessed at baseline, after three and 6 months.

Results: Twenty-one participants completed the programme. Overall, functional performance showed positive results varying from (very) small to large effects (Cohen's d 0.04-0.81), mainly during the supervised part of the intervention. Regarding daily physical activity, a slight improvement with (very) small effects (Cohen's d 0.07-0.38), was observed for both self-reported and objectively measured physical activity during the supervised period. However, during the unsupervised period this pattern only continued for self-reported physical activity.

Conclusion: This pilot study showed positive results varying from (very) small to large effects in levels and maintenance of functional performance and daily physical activity, especially during the supervised first 3 months. Remote supervision seems to importantly affect effectiveness of a home-based exercise programme. Effectiveness of the programme and the exact contribution of its components should be further quantified in a randomized controlled trial.

Practice Implications: Home-based exercising using novel technology may be promising for functional performance and physical activity improvement in (pre-frail) older adults.

Trial Registration: Netherlands Trial Register (NTR); trial number NL4049 . The study was prospectively registered (registration date 14/11/2013).
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http://dx.doi.org/10.1186/s11556-021-00264-yDOI Listing
June 2021

High quality, high index-contrast chalcogenide microdisk resonators.

Opt Express 2021 Jun;29(12):17775-17783

We demonstrate the high quality (Q) factor microdisk resonators in high index-contrast chalcogenide glass (ChG) film GeSbSe using electron-beam lithography followed by plasma dry etching. High confinement, low-loss, and single-point-coupled microdisk resonators with a loaded Q factor of 5×10 are measured. We also present pulley-coupled microdisk resonators for relaxing the requirements on the coupling gap. While adjusting the wrap-around coupling waveguides to be phase-matched to the resonator mode, a single specific microdisk radial mode can be excited. Moreover, the thermal characterization of microdisk resonators is carried out to estimate the thermo-optic coefficient of 6.7×10/K for bulk ChG.
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http://dx.doi.org/10.1364/OE.427054DOI Listing
June 2021

The effect of action observation on aesthetic preference of Chinese calligraphy: An fMRI study.

Brain Behav 2021 Jun 21. Epub 2021 Jun 21.

School of Psychology, South China Normal University, Guangzhou, Guangdong, China.

Introduction: There is some evidence suggesting that movement perception has an effect on aesthetic experience. However, the neural mechanisms underlying the observation of creators' creative action (the process that calligraphers create calligraphy) remain unclear.

Methods: In this study, participants were scanned with fMRI while performing aesthetic judgments on Chinese calligraphy images with/without action observation.

Results: Behavioral results showed that both the work by the expert and novice with action observation were rated significantly higher on aesthetic preference than those without action observation. Imaging results showed that brain regions associated with perceptual, cognitive, and emotional processing were commonly activated by calligraphy images with/without action observation. However, compared with no action observation, aesthetic judgments of calligraphy images with action observation elicited stronger activation in the anterior cingulate cortex and the bilateral insula. Meanwhile, the superior parietal lobe which is associated with relevant inner action imitation, was also activated when observing the creator's action.

Conclusions: Brain activation in the superior parietal lobe, anterior cingulate cortex, and the bilateral insula indicated that observing the creative action of the creators contributed to the aesthetic experience of the observer.
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http://dx.doi.org/10.1002/brb3.2265DOI Listing
June 2021

An integrative analysis of genome-wide 5-hydroxymethylcytosines in circulating cell-free DNA detects noninvasive diagnostic markers for gliomas.

Neurooncol Adv 2021 Jan-Dec;3(1):vdab049. Epub 2021 Apr 16.

State Key Laboratory of Medical Neurobiology, School of Basic Medical Sciences, and The Collaborative Innovation Centre for Brain Science, Fudan University, Shanghai, China.

Background: Gliomas, especially the high-grade glioblastomas (GBM), are highly aggressive tumors in the central nervous system (CNS) with dismal clinical outcomes. Effective biomarkers, which are not currently available, may improve clinical outcomes through early detection. We sought to develop a noninvasive diagnostic approach for gliomas based on 5-hydroxymethylcytosines (5hmC) in circulating cell-free DNA (cfDNA).

Methods: We obtained genome-wide 5hmC profiles using the 5hmC-Seal technique in cfDNA samples from 111 prospectively enrolled patients with gliomas and 111 age-, gender-matched healthy individuals, which were split into a training set and a validation set. Integrated models comprised 5hmC levels summarized for gene bodies, long noncoding RNAs (lncRNAs), -regulatory elements, and repetitive elements were developed using the elastic net regularization under a case-control design.

Results: The integrated 5hmC-based models differentiated healthy individuals from gliomas (area under the curve [AUC] = 84%; 95% confidence interval [CI], 74-93%), GBM patients (AUC = 84%; 95% CI, 74-94%), WHO II-III glioma patients (AUC = 86%; 95% CI, 76-96%), regardless of (encoding isocitrate dehydrogenase) mutation status or other glioma-related pathological features such as TERT, TP53 in the validation set. Furthermore, the 5hmC biomarkers in cfDNA showed the potential as an independent indicator from mutation status and worked in synergy with mutation to distinguish GBM from WHO II-III gliomas. Exploration of the 5hmC biomarkers for gliomas revealed relevance to glioma biology.

Conclusions: The 5hmC-Seal in cfDNA offers the promise as a noninvasive approach for effective detection of gliomas in a screening program.
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http://dx.doi.org/10.1093/noajnl/vdab049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209591PMC
April 2021

Hydrogen Sulfide Inhibits Enzymatic Browning of Fresh-Cut Chinese Water Chestnuts.

Front Nutr 2021 4;8:652984. Epub 2021 Jun 4.

College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, China.

This work investigates the role of hydrogen sulfide (HS) in the browning and regulating the antioxidant defensive system in fresh-cut Chinese water chestnuts. The samples were fumigated with 0, 10, and 15 μl L of HS and stored at 10°C for 8 days. The results indicated that the HS treatment significantly inhibited the browning of fresh-cut Chinese water chestnuts, reduced superoxide anion ( ) production rate and HO content accumulation, promoted the increase of total phenol content, and enhanced activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) ( < 0.05). On the other hand, phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) activities remained at a low level in the HS treatment ( < 0.05). This result suggested that HS treatment might be a promising approach to inhibit browning and prolong the shelf life by enhancing oxidation resistance and inhibiting browning enzyme activity of fresh-cut Chinese water chestnuts during storage. Among them, the 15 μl L HS treatment had the best effect on fresh-cut Chinese water chestnuts.
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http://dx.doi.org/10.3389/fnut.2021.652984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212951PMC
June 2021

Immune-Inflammatory Responses of an Acellular Cartilage Matrix Biomimetic Scaffold in a Xenotransplantation Goat Model for Cartilage Tissue Engineering.

Front Bioeng Biotechnol 2021 2;9:667161. Epub 2021 Jun 2.

Research Institute of Plastic Surgery, Weifang Medical University, Weifang, China.

The rapid development of tissue engineering and regenerative medicine has introduced a new strategy for ear reconstruction, successfully regenerating human-ear-shaped cartilage and achieving the first clinical breakthrough using a polyglycolic acid/polylactic acid (PGA/PLA) scaffold. However, its clinical repair varies greatly among individuals, and the quality of regenerated cartilage is unstable, which seriously limits further clinical application. Acellular cartilage matrix (ACM), with a cartilage-specific microenvironment, good biocompatibility, and potential to promote cell proliferation, has been used to regenerate homogeneous ear-shaped cartilage in immunocompromised nude mice. However, there is no evidence on whether ACM will regenerate homogeneous cartilage tissue in large animals or has the potential for clinical transformation. In this study, xenogeneic ACM assisted with gelatin (GT) with or without autologous chondrocytes was implanted subcutaneously into goats to establish a xenotransplantation model and compared with a PGA/PLA scaffold to evaluate the immune-inflammatory response and quality of regenerated cartilage. The results confirmed the superiority of the ACM/GT, which has the potential capacity to promote cell proliferation and cartilage formation. Although there is a slight immune-inflammatory response in large animals, it does not affect the quality of the regenerated cartilage and forms homogeneous and mature cartilage. The current study provides detailed insights into the immune-inflammatory response of the xenogeneic ACM/GT and also provides scientific evidence for future clinical application of ACM/GT in cartilage tissue engineering.
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http://dx.doi.org/10.3389/fbioe.2021.667161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208476PMC
June 2021

Comparative Transcriptome Analysis Demonstrates the Positive Effect of the Cyclic AMP Receptor Protein Crp on Daptomycin Biosynthesis in .

Front Bioeng Biotechnol 2021 4;9:618029. Epub 2021 Jun 4.

Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd., Hangzhou, China.

Daptomycin, which is produced by , has been characterized as a novel cyclic lipopeptide antibiotic that is effective against Gram-positive bacteria. The biosynthesis of daptomycin is regulated by various factors. In the present study, we demonstrated that the cyclic AMP receptor protein (Crp) plays an important role in producing daptomycin in the industrial strain. We found that daptomycin production from the deletion strain decreased drastically, whereas production from the overexpression strain increased by 22.1%. Transcriptome and qPCR analyses showed that some genes related to the daptomycin biosynthetic gene cluster () and the pleiotropic regulator () were significantly upregulated. RNA-seq also shows Crp to be a multifunctional regulator that modulates primary metabolism and enhances precursor flux to secondary metabolite biosynthesis. These results provide guidance for the development and improvement of potential natural products.
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http://dx.doi.org/10.3389/fbioe.2021.618029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212052PMC
June 2021

Combined detection of ACL and β2-GP1 in diagnosis of adverse pregnancy.

Am J Transl Res 2021 15;13(5):5653-5658. Epub 2021 May 15.

Department of Obstetrics, Hengshui People's Hospital Hengshui, China.

Objective: To illustrate the significance of combined detection of anti cardiolipin (ACL) and anti-β2-glycoprotein 1 (β2-GP1) in the diagnosis of adverse pregnancy.

Methods: 60 postnatal women with adverse pregnancy presented to our hospital from March 2019 to March 2020 were enrolled as the experimental group, and 60 postnatal women with normal delivery in the same period were selected as the control group. The levels of ACL, β2-GP1 were measured to analyze the significance of the integrated detection in the diagnosis of adverse pregnancy.

Results: No obvious differences were observed in mean age, mean gestational week, mean height, number of cases of primigravida and multiparous women, educational background, place of residence between two groups ( > 0.05). The number of cases of positive β2-GP1-IgG and β2-GP1-IgM tests in experimental group were remarkably higher ( < 0.05). The number of β2-GP1-IgA positive cases in the two kinds of parturients was not notably different ( > 0.05). The ACL of the experimental parturients had a notably higher number of β2-GP1-positive cases ( < 0.001), and the combined positive cases of the experimental parturients had notably higher serum BPA and resistin levels ( < 0.001). The incidence of fetal growth restriction, premature delivery and placental abruption of experimental group were higher than those of control group (P < 0.05). There was no stillbirth in both groups.

Conclusions: The positive expression of ACL with β2-GP1 is the culprit of adverse pregnancy, and the combined diagnosis can greatly improve the clinical screening rate of adverse pregnancy and provide more evidence for subsequent treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205803PMC
May 2021

Effects of PICCO in the guidance of goal-directed fluid therapy for gastrointestinal function after cytoreductive surgery for ovarian cancer.

Am J Transl Res 2021 15;13(5):4852-4859. Epub 2021 May 15.

Department of Anesthesiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China Hefei, Anhui Province, China.

Objective: To explore the effects of PICCO on the Guidance of Goal-Directed Fluid Therapy for gastrointestinal function after cytoreductive surgery for ovarian cancer.

Methods: A total of 98 patients who received cytoreductive surgery for ovarian cancer under general anesthesia in our hospital were stochastically divided into the observation group and control group, with 49 cases in each group. The observation group received PICCO guided goal-directed fluid therapy, while patients in the control group were treated with conventional fluid therapy. At last, the total amount of infusion, the time required for postoperative gastrointestinal function recovery and related recovery rating, the first time to get out of bed after the surgery and the total hospitalization time were observed and compared between the two groups.

Results: The observation group had less time for gastrointestinal function recovery and had better functional recovery rating as well as other indicators than the control group. Besides, the observation group had less volume of infusion, needed shorter time to get out of bed and had a shorter length of hospital stay than the control group (all P<0.05).

Conclusion: PICCO guided goal-directed liquid therapy can effectively improve the recovery of gastrointestinal function and reduce the length of stay in patients with ovarian cancer after cytoreductive surgery.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205791PMC
May 2021

A brief diagnostic screen for cluster headache: Creation and initial validation of the Erwin Test for Cluster Headache.

Cephalalgia 2021 Jun 20:3331024211018138. Epub 2021 Jun 20.

Department of Neurosurgery, 12340University of Texas Health Science Center at Houston, Houston, TX, USA.

Objective: To use 1) newly generated data, 2) existing evidence, and 3) expert opinion to create and validate a new cluster headache screening tool.

Methods: In phase 1 of the study, we performed a prospective study of an English translation of an Italian screen on 95 participants (45 with cluster headache, 17 with other trigeminal autonomic cephalalgias, 30 with migraine, and 3 with trigeminal neuralgia). In phase 2, we performed a systematic review in PubMed of all studies until September 2019 with diagnostic screening tools for cluster headache. In phase 3, a 6-person panel of cluster headache patients, research coordinators, and headache specialists analyzed the data from the first two phases to generate a new diagnostic screening tool. Finally, in phase 4 this new screen was validated on participants at a single headache center (all diagnoses) and through research recruitment (trigeminal autonomic cephalalgias only, as recruitment was essential but was otherwise low).

Results: In total, this study included 319 unique participants including 109 cluster headache participants (95 total participants/45 cluster headache participants in phase 1, and 224 total participants/64 cluster headache participants in phase 4). It also found 123 articles on potential screening tools in our systematic review. In phase 1, analysis of the English translation of an Italian screen generated 7 questions with high sensitivity and specificity against migraine, trigeminal neuralgia, and other trigeminal autonomic cephalalgias, but had grammatical and other limitations as a general screening tool. In phase 2, the systematic review revealed nine studies that met inclusion criteria as diagnostic screening tools for cluster headache, including four where sensitivity and specificity were available for individual questions or small groups of questions. In phase 3, this data was reviewed by the expert panel to generate a brief (6-item), binary (yes/no), written screening test. In phase 4, a total of 224 participants completed the new 6-item screening test (81 migraine, 64 cluster headache, 21 other trigeminal autonomic cephalalgias, 35 secondary headaches, 7 neuralgias, 5 probable migraine, and 11 other headache disorders). Answers to the 6 items were combined in a decision tree algorithm and three items had a sensitivity of 84% (confidence interval or 95% confidence interval 73-92%), specificity of 89% (95% confidence interval 84-94%), positive predictive value of 76% (95% confidence interval 64-85%), and negative predictive value of 93% (95% confidence interval 88-97%) for the diagnosis of cluster headache. These three items focused on headache intensity, duration, and autonomic features.

Conclusion: The 3-item Erwin Test for Cluster Headache is a promising diagnostic screening tool for cluster headache.
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http://dx.doi.org/10.1177/03331024211018138DOI Listing
June 2021

Bisphenol F promotes the secretion of pro-inflammatory cytokines in macrophages by enhanced glycolysis through PI3K-AKT signaling pathway.

Toxicol Lett 2021 Jun 17. Epub 2021 Jun 17.

Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, 523808, Guangdong Province, China; Liaobu Hospital, Guangdong Medical University, Dongguan, 523808, Guangdong Province, China. Electronic address:

Bisphenol F (BPF) is a member of endocrine disrupting chemicals (EDCs). As a substitute of bisphenol A (BPA), BPF is widely used in various consumer products, leading to an increased risk of people's exposure. However, there are few studies on the immunotoxicity and mechanism of BPF. This study aimed to investigate the effect of BPF on the secretion of pro-inflammatory cytokines by macrophages and explore its mechanism. In our study, RAW264.7 macrophages were treated with different concentrations of BPF (0, 5, 10 and 20 μM) for 24 h. The results showed that the secretion of pro-inflammatory cytokines (IL-6, TNF-α and IL-1β) and the production of lactate were increased in a dose-dependent manner. BPFalso led to the activation of the PI3K-AKT signaling pathway. After pretreatment with glycolysis inhibitor (2-DG) and exposure to BPF (20 μM), the secretion of pro-inflammatory cytokines induced by BPF was inhibited. PI3K inhibitor (LY294002) and estrogen receptor (ER) antagonist (ICI 182,780) could also inhibit the above effects induced by BPF (20 μM). In conclusion, our results suggested that BPF can enhance glycolysis through ER mediated PI3K-AKT signaling pathway, and the enhanced glycolysis further promoted the secretion of pro-inflammatory cytokines. Our research provides basic data for future studies on bisphenol exposure and immunotoxicity.
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http://dx.doi.org/10.1016/j.toxlet.2021.06.011DOI Listing
June 2021

Targeting matrix stiffness-induced activation of retinal pigment epithelial cells through the RhoA/YAP pathway ameliorates proliferative vitreoretinopathy.

Authors:
Wei Zhang Han Han

Exp Eye Res 2021 Jun 18;209:108677. Epub 2021 Jun 18.

Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, 300052, China.

The purpose of this study was to investigate whether excessive extracellular matrix (ECM) deposition-induced mechanical matrix stiffness plays a key role in promoting retinal pigment epithelial (RPE) cell activation and the subsequent development of proliferative vitreoretinopathy (PVR). Human ARPE-19 cells were cultured on either 50 kappa (stiff) or 0.5 kappa (soft) gel-coated coverslips. Reverse and knockdown experiments were carried out to establish a model of matrix stiffness-induced activation in ARPE-19 cells in vitro. A PVR mouse model was established by the intravitreal injection of dispase. The effects of RhoA/YAP signalling blockade on matrix stiffness-induced ARPE-19 cell activation and PVR-induced retinal fibrosis were determined by using a combination of the Yes-associated protein (YAP) inhibitor verteporfin and the RhoA inhibitor C3 exoenzyme. Matrix stiffness stimulated YAP nuclear translocation and expression in ARPE-19 cells. The effect of YAP activation was dependent on F-actin cytoskeleton polymerization and RhoA activity, forming the RhoA/YAP signalling pathway. Upstream pharmacological blockade of RhoA by C3 exoenzyme or downstream blockade of YAP by verteporfin reduced the invasion, migration, and MMP expression of ARPE-19 cells and collagen gel contraction. Furthermore, blockade of RhoA/YAP signalling reduced PVR-induced retinal fibrogenesis and inhibited the TGF-β/Smad pathway in vivo. RhoA/YAP signalling modulates matrix stiffness-induced activation of ARPE-19 cells. Targeting this signalling pathway could alleviate PVR-induced retinal fibrosis and suggests attractive novel therapeutic strategies for intervening in the progression of PVR.
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http://dx.doi.org/10.1016/j.exer.2021.108677DOI Listing
June 2021

Sequence-Based Prediction of Transmembrane Protein Crystallization Propensity.

Interdiscip Sci 2021 Jun 18. Epub 2021 Jun 18.

Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, China.

Transmembrane proteins play a vital role in cell life activities. There are several techniques to determine transmembrane protein structures and X-ray crystallography is the primary methodology. However, due to the special properties of transmembrane proteins, it is still hard to determine their structures by X-ray crystallography technique. To reduce experimental consumption and improve experimental efficiency, it is of great significance to develop computational methods for predicting the crystallization propensity of transmembrane proteins. In this work, we proposed a sequence-based machine learning method, namely Prediction of TransMembrane protein Crystallization propensity (PTMC), to predict the propensity of transmembrane protein crystallization. First, we obtained several general sequence features and the specific encoded features of relative solvent accessibility and hydrophobicity. Second, feature selection was employed to filter out redundant and irrelevant features, and the optimal feature subset is composed of hydrophobicity, amino acid composition and relative solvent accessibility. Finally, we chose extreme gradient boosting by comparing with other several machine learning methods. Comparative results on the independent test set indicate that PTMC outperforms state-of-the-art sequence-based methods in terms of sensitivity, specificity, accuracy, Matthew's Correlation Coefficient (MCC) and Area Under the receiver operating characteristic Curve (AUC). In comparison with two competitors, Bcrystal and TMCrys, PTMC achieves an improvement by 0.132 and 0.179 for sensitivity, 0.014 and 0.127 for specificity, 0.037 and 0.192 for accuracy, 0.128 and 0.362 for MCC, and 0.027 and 0.125 for AUC, respectively.
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http://dx.doi.org/10.1007/s12539-021-00448-1DOI Listing
June 2021

A Novel Mechanism of Action of Histone Deacetylase Inhibitor Chidamide: Enhancing the Chemotaxis Function of Circulating PD-1(+) Cells From Patients With PTCL.

Front Oncol 2021 1;11:682436. Epub 2021 Jun 1.

Department of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Background: Peripheral T-cell lymphomas (PTCLs) are a heterogeneous group of neoplasms characterized by a poor prognosis. Histone deacetylase (HDAC) inhibitors have emerged as novel therapeutic agents for PTCLs. In this study, we aimed to explore the immunomodulatory effect of the HDAC inhibitor chidamide on circulating PD-1(+) cells from patients with PTCL, as well as its correlation with treatment response.

Methods: We enrolled newly diagnosed patients with PTCLs treated with a combination of chidamide and chemotherapy. Gene expression profile analysis was performed on peripheral blood PD-1(+) cells, both at baseline and at the end of treatment. A list of differentially expressed genes (DEGs) was identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to annotate the biological implications of the DEGs. A gene concept network was constructed to identify the key DEGs for further PCR verification.

Results: A total of 302 DEGs were identified in the complete remission (CR) group, including 162 upregulated and 140 downregulated genes. In contrast, only 12 DEGs were identified in the non-CR group. GO analysis revealed that these upregulated DEGs were mainly involved in chemokine activity, cell chemotaxis, and cellular response to interleukin-1 and interferon-γ. Furthermore, KEGG pathway analysis showed that these DEGs were enriched in cytokine-cytokine receptor interaction and chemokine signaling pathways. The innate immune signaling pathways, including the Toll-like and NOD-like receptor signaling pathways, were also influenced. The gene concept network revealed that the key upregulated genes belonged to the C-C chemokine family.

Conclusion: Our results showed that chidamide treatment notably enhanced the expression of genes associated with chemokine activity and chemotaxis function of circulating PD-1(+) cells. By recruiting immune cells and improving the innate immune function of PD-1(+) cells, chidamide may reshape the tumor microenvironment to an anti-tumor phenotype and synergize with checkpoint inhibitors.
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http://dx.doi.org/10.3389/fonc.2021.682436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204089PMC
June 2021

A colorimetric and fluorescence turn-on probe for the detection of ascorbic acid in living cells and beverages.

Food Chem 2021 Jun 9;363:130325. Epub 2021 Jun 9.

College of Science, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. Electronic address:

A colorimetric and fluorescence turn-on dual-signal assay was developed for the determination of ascorbic acid (AA). Because the ultraviolet absorption of the oxidized 3,3',5,5'-tetramethylbenzidine (oxTMB) overlapped with the fluorescence emission of glutathione stabilized Au nanoclusters (AuNCs), the fluorescence of AuNCs can be quenched by oxTMB. When AA was added, the blue oxTMB was reduced to colorless TMB, and the fluorescence of AuNCs was restored simultaneously. The decrease in absorbance and increase in fluorescence signal depended on the concentration of AA. In the determination range of 0.5 to 200 μM, the detection limits (LOD) for AA were as low as 0.15 µM and 0.22 µM for fluorometric and colorimetric, respectively. The established probe was used successfully for AA detection in living cells and beverages.
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http://dx.doi.org/10.1016/j.foodchem.2021.130325DOI Listing
June 2021

Vibration warning design for reaction time reduction under the environment of intelligent connected vehicles.

Appl Ergon 2021 Jun 14;96:103490. Epub 2021 Jun 14.

State Key Laboratory of Automotive Safety and Energy, Department of Industrial Engineering, Tsinghua University, Beijing, China. Electronic address:

Scenario-based warnings for road safety can be provided in the environment of intelligent connected vehicles via Bluetooth earphones or smart wristbands; designing an optimal means of presentation to drivers is an important point of consideration. Vibration warnings have been widely studied owing to their unique benefits. This experimental study aims to identify suitable body parts for vibration warnings during driving. The independent variables were the vibration position (three levels of stimulus, i.e., wrist, shin, and upper jaw) and response effector (two levels, i.e., hand and foot). Experiment Tasks 1 and 2 measured participants' simple reaction time and choice reaction time, respectively, when providing vibration warnings in non-driving situations. The results demonstrate that the vibration on the upper jaw has the shortest simple reaction time and choice reaction time. The effect of stimulus-response consistency on choice reaction time was insignificant. Task 3 was similar to Task 2, with the exception of simulated driving. Compared to the result in Task 2, the choice reaction time in Task 3 was approximately 200 ms longer. Vibration of the upper jaw was reported to have the highest perceived intensity and preference. Based on the study results, the design implications for wearable vibration warnings of collision avoidance systems are presented.
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http://dx.doi.org/10.1016/j.apergo.2021.103490DOI Listing
June 2021

Bismuth-Based Free-Standing Electrodes for Ambient-Condition Ammonia Production in Neutral Media.

Nanomicro Lett 2020 Jun 19;12(1):133. Epub 2020 Jun 19.

Discipline of Chemistry, University of Newcastle, Callaghan, NSW, 2308, Australia.

Electrocatalytic nitrogen reduction reaction is a carbon-free and energy-saving strategy for efficient synthesis of ammonia under ambient conditions. Here, we report the synthesis of nanosized BiO particles grown on functionalized exfoliated graphene (BiO/FEG) via a facile electrochemical deposition method. The obtained free-standing BiO/FEG achieves a high Faradaic efficiency of 11.2% and a large NH yield of 4.21 ± 0.14 [Formula: see text] h cm at - 0.5 V versus reversible hydrogen electrode in 0.1 M NaSO, better than that in the strong acidic and basic media. Benefiting from its strong interaction of Bi 6p band with the N 2p orbitals, binder-free characteristic, and facile electron transfer, BiO/FEG achieves superior catalytic performance and excellent long-term stability as compared with most of the previous reported catalysts. This study is significant to design low-cost, high-efficient Bi-based electrocatalysts for electrochemical ammonia synthesis.
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http://dx.doi.org/10.1007/s40820-020-00444-yDOI Listing
June 2020

The global emergence of a novel Streptococcus suis clade associated with human infections.

EMBO Mol Med 2021 Jun 17:e13810. Epub 2021 Jun 17.

Key Laboratory of Environment Correlative Dietology, Interdisciplinary Sciences Institute, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.

Streptococcus suis, a ubiquitous bacterial colonizer in pigs, has recently extended host range to humans, leading to a global surge of deadly human infections and three large outbreaks since 1998. To better understand the mechanisms for the emergence of cross-species transmission and virulence in human, we have sequenced 366 S. suis human and pig isolates from 2005 to 2016 and performed a large-scale phylogenomic analysis on 1,634 isolates from 14 countries over 36 years. We show the formation of a novel human-associated clade (HAC) diversified from swine S. suis isolates. Phylogeographic analysis identified Europe as the origin of HAC, coinciding with the exportation of European swine breeds between 1960s and 1970s. HAC is composed of three sub-lineages and contains several healthy-pig isolates that display high virulence in experimental infections, suggesting healthy-pig carriers as a potential source for human infection. New HAC-specific genes are identified as promising markers for pathogen detection and surveillance. Our discovery of a human-associated S. suis clade provides insights into the evolution of this emerging human pathogen and extend our understanding of S. suis epidemics worldwide.
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http://dx.doi.org/10.15252/emmm.202013810DOI Listing
June 2021

Jasmonate signaling restricts root soluble sugar accumulation and drives root-fungus symbiosis loss at flowering by antagonizing gibberellin biosynthesis.

Plant Sci 2021 Aug 19;309:110940. Epub 2021 May 19.

Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, China. Electronic address:

Jasmonate restricts accumulation of constitutive and fungus-induced root soluble sugars at flowering stage, and thus reduces root beneficial fungal colonization, but little is known about how these are achieved. To determine whether jasmonate-mediated depletion of soluble sugars is the result of direct phytohormonal cross-talk or indirect induced defensive secondary metabolism, we first profiled soluble sugar and tryptophan (Trp)-derived defensive secondary metabolites in the roots of wild-type and jasmonate signaling-impaired Arabidopsis thaliana at flowering upon a beneficial fungus Phomopsis liquidambaris inoculation. Next, jasmonate and gibberellin signaling were manipulated to determine the relationship between jasmonate and gibberellin, and to quantify the effects of these phytohormones on fungal colonization degree, soluble sugar accumulation, Trp-derived secondary metabolites production, and sugar source-sink transport and metabolism. Gibberellin complementation increased Ph. liquidambaris colonization and rescued jasmonate-dependent root soluble sugar depletion and phloem sugar transport and root invertase activity without influencing jasmonate-induced Trp-derived secondary metabolites production at flowering. Furthermore, jasmonate signaling antagonized gibberellin biosynthesis in Ph. liquidambaris-inoculated roots. Our results suggest a phytohormonal antagonism model that jasmonate signaling restricts root soluble sugar accumulation through antagonizing gibberellin biosynthesis rather than through promoting Trp-derived secondary metabolites production and thus drives beneficial fungal colonization decline at flowering.
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http://dx.doi.org/10.1016/j.plantsci.2021.110940DOI Listing
August 2021

CELL CYCLE SEITCH 52 regulates tillering by interacting with LATERAL SUPPRESSOR in non-heading Chinese cabbage.

Plant Sci 2021 Aug 3;309:110934. Epub 2021 May 3.

State Key Laboratory of Crop Genetics & Germplasm Enhancement, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of the P.R. China, China; Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of the P.R. China, Nanjing, 210095, China. Electronic address:

With the discovery of essential genes regulating tillering, such as MONOCULM 1 (MOC1) in rice and LATERAL SUPPRESSOR (LAS in Arabidopsis, LS in tomato), research on tillering mechanisms has made great progress; however, the study of tillering in non-heading Chinese cabbage (NHCC) is rare. Here, we report that BcLAS, as a member of the GRAS family, plays an important role in the tillering of NHCC during its vegetative growth. BcLAS was almost not expressed in other examed parts except leaf axils throughout life. When the expression of BcLAS was silenced utilizing virus-induced gene silencing (VIGS) technology, we found that the tiller number of 'Maertou' decreased sharply. In 'Suzhouqing', overexpression of BcLAS significantly promoted tillering. BcCCS52, the orthologue to CELL CYCLE SEITCH 52 (CCS52), interacts with BcLAS. Downregulation of the expression of BcCCS52 promoted tillering of 'Suzhouqing'; therefore, we conclude that BcCCS52 plays a negative role in tillering regulation. Our findings reveal the tillering regulation mechanism of NHCCs at the vegetative stage and report an orthologue of CCS52 regulating tillering in NHCC.
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http://dx.doi.org/10.1016/j.plantsci.2021.110934DOI Listing
August 2021

The largest secure corridor of the infra-acetabular screw-a 3-D axial perspective analysis.

BMC Musculoskelet Disord 2021 Jun 16;22(1):551. Epub 2021 Jun 16.

Department of Traumatic Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

Background: The infra-acetabular screw which is placed from the pubis to the ischium can be used as a special positional screw of the posterior column of the acetabulum. This study was performed to simulate the surgical procedure and obtain the ideal insertion point, diameter, length and angle of the screw through the method of axial perspective in Chinese patients.

Methods: We randomly collected the pelvic computed tomography (CT) scans of 200 adults. DICOM-formatted CT-scan images were imported into Mimics software to establish the 3D digital model of the right semi-pelvic was established. A virtual cylinder representing the screw was placed from the pubis to the ischium to fix the posterior column. The largest secure diameter and length of the virtual screw were measured and the position of the insertion point and the directions of the screw were also researched.

Results: The screw insertion safe zone exhibits an irregular shape of "tear drop" in the reconstructed pelvic model. The mean maximum diameter of screws was 5.01 ± 1.28 mm, and the mean maximum length of screws was 93.99 ± 8.92 mm. The screw insertion corridor with the least diameter 3.5 mm was found in 94 of 100 males (94%) and 86 of 100 females (86%). We found gender-dependent differences for the mean maximum diameter and the maximum length of the screw. There was statistically significant difference between genders in the position of insertion point.

Conclusions: In this study, we suggest an individual preoperative 3D reconstruction simulation to develop better screw placement plans, which provides a valuable guideline for seeking the largest secure corridor of infra-acetabular screw. Further biomechanical studies are needed to verify the function of the screw.
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http://dx.doi.org/10.1186/s12891-021-04433-zDOI Listing
June 2021

[Retracted] Additive effects of eukaryotic co‑expression plasmid carrying GRIM‑19 and LKB1 genes on breast cancer and .

Mol Med Rep 2021 Aug 16;24(2). Epub 2021 Jun 16.

Department of Cosmetology Plastic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that the western blotting data featured in Figs. 1C and 4C, and tumour images in Fig. 5A, were strikingly similar to data appearing in different form in other articles by different authors at different research institutes. Owing to the fact that the contentious data in the above article were already under consideration for publication, or had already been published, elsewhere prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors did not reply to indicate whether or not they agreed with the retraction of the paper. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 12: 7665‑7672, 2015; DOI: 10.3892/mmr.2015.4393].
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http://dx.doi.org/10.3892/mmr.2021.12226DOI Listing
August 2021

[Retracted] Upregulation of GRIM‑19 inhibits the growth and invasion of human breast cancer cells.

Mol Med Rep 2021 Aug 16;24(2). Epub 2021 Jun 16.

Department of Cosmetology Plastic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that the western blotting data featured in Figs. 4B and 6B (wherein there was also a duplicated set of data bands) were strikingly similar to data appearing in different form in other articles at different research institutes. Owing to the fact that the contentious data in the above article were already under consideration for publication, or had already been published, elsewhere prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors did not reply to indicate whether or not they agreed with the retraction of the paper. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 10.3892/mmr.2015.3757].
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http://dx.doi.org/10.3892/mmr.2021.12225DOI Listing
August 2021

Efficient heavy metal removal from water by alginate-based porous nanocomposite hydrogels: The enhanced removal mechanism and influencing factor insight.

J Hazard Mater 2021 Jun 8;418:126358. Epub 2021 Jun 8.

School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha 410114, China; Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha 410114, China. Electronic address:

Novel porous alginate-based nanocomposite hydrogels were prepared by incorporating polyaniline-polypyrrole modified graphene oxide ([email protected]) as reinforcing fillers into the alginate matrix ([email protected]/SA) for Cr(VI) and Cu(II) removal from water. Different in-situ co-polymerization functionalized GO with Py-to-An mass ratios of monomers (from nil to 1:1) and contents of [email protected] (from nil to 2.0%(w/v)) were embedded into the alginate backbone to improve the sorption performance. Key factors, such as pH, coexisting metal ions, and swelling states were investigated in batch adsorption modes. The synergistic effect combined from polymer backbone and fillers could lower the impact of the pH-dependent adsorption reaction. With an adsorption ability superior to that of plain SA and GO/SA, the optimized [email protected](1)/SA exhibited good experimental maximum adsorption capacities for Cr(VI) (~133.7 mg/g) and Cu(II) (~87.2 mg/g) at pH 3.0, which were better than those of many other similar sorbents. The sorbents possessed excellent adaptability for 0.2 M salt for Cr(VI) removal but poor for Cu(II) removal. Pre-swelling treatment and co-adsorption could enhance the adsorption performance. The excellent reusability of hydrogel was demonstrated after five cycles in single/binary system. Overall, this work reveals that the resultant hydrogel holds potential as candidate sorbent to remove anionic-cationic heavy metal ions from water.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126358DOI Listing
June 2021

An Escherichia coli isolate from hospital sewage carries bla and bla.

Arch Microbiol 2021 Jun 15. Epub 2021 Jun 15.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

Carbapenems, as the "last line of defense" against Gram-negative bacteria, are increasingly being challenged by drug-resistant bacteria, especially Enterobacteriaceae. In this study, a carbapenem-resistant Gram-negative bacterium, named AH001, was isolated from hospital sewage, and a modified Hodge test confirmed that this bacterium can produce carbapenemase. Further analysis revealed that this bacterium exhibits multidrug resistance against an additional seven antibiotics. Whole-genome sequencing and analysis showed that AH001 could not be classified by existing MLST, and its serotype could not be distinguished among O9, O89 or O168 according to O antigen prediction. More attention should be given to the role of environmental sources of Escherichia coli in the development and transfer of drug resistance in the hospital environment.
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http://dx.doi.org/10.1007/s00203-021-02431-2DOI Listing
June 2021

Brachial-ankle pulse wave velocity and prognosis in patients with atherosclerotic cardiovascular disease: a systematic review and meta-analysis.

Hypertens Res 2021 Jun 14. Epub 2021 Jun 14.

Department of Special Care Center, Fuwai Hospital, National Clinical Research Center for Cardiovascular Diseases, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Arterial stiffness has been suggested as an independent cardiovascular risk factor. This systematic review and meta-analysis aimed to quantify the association between brachial-ankle pulse wave velocity (baPWV) and prognosis in atherosclerotic cardiovascular disease (ASCVD) patients. The PubMed, EMBASE, and Cochrane Library databases were searched for cohort studies examining the association of high baPWV with prognosis in ASCVD patients. High baPWV was defined by the cutoffs provided by each study. The outcomes of interest were cardiovascular events, cardiovascular mortality, and all-cause mortality. Hazard ratios (HRs) and 95% confidence intervals (CIs) were combined using the random-effects model with inverse variance weighting. We identified 15 studies that were eligible for inclusion in the meta-analysis. The overall HRs and 95% CIs of cardiovascular events, cardiovascular mortality, and all-cause mortality for high baPWV were 2.55 (1.61-4.03), 2.66 (1.88-3.76), and 1.77 (1.09-2.87), respectively. The association between baPWV and cardiovascular events remained significant, irrespective of determination methods for cutoffs of baPWV, classification of ASCVD, outcome definitions, Newcastle-Ottawa Scale score and average age, independent of age and hypertension. Significantly higher HRs were observed in the subgroups of >3 years follow-up duration (p for interaction: 0.04), cutoff points by ROC curves (p for interaction: 0.04) and an average age of <65 years (p for interaction: 0.01). A 1 standard deviation increase in baPWV was associated with a 1.41-fold (1.24-1.60) increase in the risk of cardiovascular events. High baPWV is independently associated with an increased risk of cardiovascular events, cardiovascular mortality, and all-cause mortality in ASCVD patients.
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http://dx.doi.org/10.1038/s41440-021-00678-2DOI Listing
June 2021

Exploring different mechanisms of biochars in removing hexavalent chromium: Sorption, reduction and electron shuttle.

Bioresour Technol 2021 Jun 10;337:125382. Epub 2021 Jun 10.

State Environmental Protection Engineering Center for Urban Soil Contamination Control and Remediation, Shanghai Academy of Environmental Sciences, Shanghai 200233, China.

The role of different biochars in Cr(VI) removal process is not clear. Two raw materials with distinct components were prepared into biochars at various pyrolysis temperatures. The biochar derived from cotton stark (with less lignin than walnut shell) always had more surface functional groups. The pyrolysis temperature had a significant effect on the biochar. In the sorption experiments, the fitting results of two compartment models showed that slow compartment dominated by surface functional groups plays a leading role in the Cr(VI) removal by low-temperature char, while the contribution of the rapid or slow compartment to the process of high-temperature char was equal. In the experiment of biochar mediated the lactate to reduce Cr(VI), the biochar derived from walnut shell (richer lignin) had better conductivity due to its highly aromatic structure, which could directly transfer electrons. This study indicated that various biochars may play different roles in the Cr(VI) removal process.
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http://dx.doi.org/10.1016/j.biortech.2021.125382DOI Listing
June 2021