Publications by authors named "Wei Zhang"

17,525 Publications

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Genomic characterization of Streptococcus parasuis, a close relative of Streptococcus suis and also a potential opportunistic zoonotic pathogen.

BMC Genomics 2022 Jun 25;23(1):469. Epub 2022 Jun 25.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

Streptococcus parasuis (S. parasuis) is a close relative of Streptococcus suis (S. suis), composed of former members of S. suis serotypes 20, 22 and 26. S. parasuis could infect pigs and cows, and recently, human infection cases have been reported, making S. parasuis a potential opportunistic zoonotic pathogen. In this study, we analysed the genomic characteristics of S. parasuis, using pan-genome analysis, and compare some phenotypic determinants such as capsular polysaccharide, integrative conjugative elements, CRISPR-Cas system and pili, and predicted the potential virulence genes by associated analysis of the clinical condition of isolated source animals and genotypes. Furthermore, to discuss the relationship with S. suis, we compared these characteristics of S. parasuis with those of S. suis. We found that the characteristics of S. parasuis are similar to those of S. suis, both of them have "open" pan-genome, their antimicrobial resistance gene profiles are similar and a srtF pilus cluster of S. suis was identified in S. parasuis genome. But S. parasuis still have its unique characteristics, two novel pilus clusters are and three different type CRISPR-Cas system were found. Therefore, this study provides novel insights into the interspecific and intraspecific genetic characteristics of S. parasuis, which can be useful for further study of this opportunistic pathogen, such as serotyping, diagnostics, vaccine development, and study of the pathogenesis mechanism.
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http://dx.doi.org/10.1186/s12864-022-08710-6DOI Listing
June 2022

Sappanwood-derived polyphenolic antidote of amyloidal toxins achieved detoxification via inhibition/reversion of amyloidal fibrillation.

Int J Biol Macromol 2022 Jun 22. Epub 2022 Jun 22.

Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA.

The formidable virulence of methicillin-resistant staphylococcus aureus (MRSA) have thrown great challenges to biomedicine, which mainly derives from their autocrine phenol-soluble modulins (PSMs) toxins, especially the most toxic member termed phenol-soluble modulins α3 (PSMα3). PSMα3 cytotoxicity is attributed to its amyloidal fibrillation and subsequent formation of cross-α sheet fibrils. Inspired by the multiple biological activity of Sappanwood, herein, we adopted brazilin, a natural polyphenolic compound originated from Caesalpinia sappan, as a potential antidote of PSMα3 toxins, and attempted to prove that the regulation of PSMα3 fibrillation was an effective alexipharmic way for MRSA infections. In vitro results revealed that brazilin suppressed PSMα3 fibrillation and disassembled preformed amyloidal fibrils in a dose-dependent manner, in which molar ratio (brazilin: PSMα3) of efficient inhibition and disassembly were both 1:1. These desired regulations dominated by brazilin benefited from its bonding to core fibrils-forming residues of PSMα3 monomers urged by hydrogen bonding and pi-pi stacking, and such binding modes facilitated brazilin-mediated inhibition or disruption of interactions between neighboring PSMα3 monomers. In this context, these inhibited and disassembled PSMα3 assemblies could not easily insert into cell membrane and subsequent penetration, and thus alleviating the membrane disruption, cytoplasmic leakage, and reactive oxygen species (ROS) generation in normal cells. As such, brazilin dramatically decreased the cytotoxicity borne by toxic PSMα3 fibrils. In addition, in vivo experiments affirmed that brazilin relieved the toxicity of PSMα3 toxins and thus promoted the skin wound healing of mice. This study provides a new antidote of PSMα3 toxins, and also confirms the feasibility of the assembly-regulation strategy in development of antidotes against supramolecular fibrillation-dependent toxins.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.141DOI Listing
June 2022

CancerCellTracker: A Brightfield Time-lapse Microscopy Framework for Cancer Drug Sensitivity Estimation.

Bioinformatics 2022 Jun 25. Epub 2022 Jun 25.

Department of Computer Science, University of Central Florida, Orlando, FL 32816, USA.

Motivation: Time-lapse microscopy is a powerful technique that relies on images of live cells cultured ex vivo that are captured at regular intervals of time to describe and quantify their behavior under certain experimental conditions. This imaging method has great potential in advancing the field of precision oncology by quantifying the response of cancer cells to various therapies and identifying the most efficacious treatment for a given patient. Digital image processing algorithms developed so far require high-resolution images involving very few cells originating from homogeneous cell line populations. We propose a novel framework that tracks cancer cells to capture their behavior and quantify cell viability to inform clinical decisions in a high-throughput manner.

Results: The brightfield microscopy images a large number of patient-derived cells in an ex vivo reconstruction of the tumor microenvironment treated with 31 drugs for up to six days. We developed a robust and user-friendly pipeline CancerCellTracker that detects cells in co-culture, tracks these cells across time, and identifies cell death events using changes in cell attributes. We validated our computational pipeline by comparing the timing of cell death estimates by CancerCellTracker from brightfield images and a fluorescent channel featuring ethidium homodimer. We benchmarked our results using a state-of-the-art algorithm implemented in ImageJ and previously published in the literature. We highlighted CancerCellTracker's efficiency in estimating the percentage of live cells in the presence of bone marrow stromal cells.

Availability And Implementation: https://github.com/compbiolabucf/CancerCellTracker.
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http://dx.doi.org/10.1093/bioinformatics/btac417DOI Listing
June 2022

TaSRO1 plays a dual role in suppressing TaSIP1 to fine tune mitochondrial retrograde signaling and enhance salinity stress tolerance.

New Phytol 2022 Jun 24. Epub 2022 Jun 24.

The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, P. R. China.

Initially discovered in yeast, mitochondrial retrograde signaling has long been recognized as an essential in the perception of stress by eukaryotes. However, how to maintain the optimal amplitude and duration of its activation under natural stress conditions remains elusive in plants. Here, we show that TaSRO1, a major contributor to the agronomic performance of bread wheat plants exposed to salinity stress, interacted with a transmembrane domain-containing NAC transcription factor TaSIP1, which could translocate from the endoplasmic reticulum (ER) into the nucleus and activate a number of mitochondrial dysfunction stimulon (MDS) genes. Over-expression of TaSIP1 and TaSIP1-∆C (a form lacking the transmembrane domain) in wheat both compromised the plants' tolerance of salinity stress, highlighting the importance of precise regulation of this signal cascade during salinity stress. The interaction of TaSRO1/TaSIP1, in the cytoplasm, arrested more TaSIP1 on the membrane of ER, and in the nucleus, attenuated the trans-activation activity of TaSIP1, thus reducing the TaSIP1-mediated activation of MDS genes. Moreover, the over-expression of TaSRO1 rescued the inferior phenotype induced by TaSIP1 over-expression. Our study provides an orchestrating mechanism executed by the TaSRO1-TaSIP1 module that balances the growth and stress response via fine-tuning the level of mitochondria retrograde signaling.
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http://dx.doi.org/10.1111/nph.18340DOI Listing
June 2022

Nanosilicate-Reinforced Silk Fibroin Hydrogel for Endogenous Regeneration of both Cartilage and Subchondral Bone.

Adv Healthc Mater 2022 Jun 24:e2200602. Epub 2022 Jun 24.

School of Medicine, Southeast University, Nanjing, 210009, China.

Osteochondral defects are characterized by injuries to both cartilage and subchondral bone, which is a result of trauma, inflammation, or inappropriate loading. Due to the unique biological properties of subchondral bone and cartilage, developing a tissue engineering scaffold that can promote dual-lineage regeneration of cartilage and bone simultaneously remains a great challenge. In this study, we fabricated a microporous nanosilicate-reinforced enzymatically-crosslinked silk fibroin (SF) hydrogel by introducing two-dimensional montmorillonite (MMT) nanoparticles via intercalation chemistry. In vitro studies showed that SF-MMT nanocomposite hydrogel had improved mechanical properties and hydrophilicity, as well as the bioactivities to promote the osteogenic differentiation of bone marrow stem cells (BMSCs) with an increased ALP activity and calcium deposition and maintain chondrocyte phenotype with a higher aggrecan expression and a lower matrix-degrading enzyme expression (MMP3/13, ADAMTS4/5) compared with SF hydrogel. Global proteomic analysis verified the dual-lineage bioactivities of SF-MMT nanocomposite hydrogel, which were probably regulated by multiple signaling pathways. Besides, it was observed that the biophysical interaction of cells and SF-MMT nanocomposite hydrogel was partially mediated by clathrin-mediated endocytosis and its downstream processes. In vivo, SF-MMT nanocomposite hydrogel effectively promoted subchondral bone and cartilage regeneration in rabbit osteochondral defect model as evidenced by macroscopic, micro-CT, and histological evaluation. In conclusion, we developed a functionalized SF-MMT nanocomposite hydrogel with dual-lineage bioactivity for osteochondral regeneration, indicating its potential in osteochondral tissue engineering. Statement of significance Osteochondral defects involve the injuries of both articular cartilage and subchondral bone. Due to the distinct properties of these two tissues, it remains a great challenge to induce the dual-lineage regeneration of cartilage and bone simultaneously. This study developed an SF-MMT nanocomposite hydrogel by enzymatic crosslinking and intercalation chemistry, which effectively enhanced the osteogenic differentiation of BMSCs and the maintenance of chondrocyte phenotype, thereby promoting the dual-lineage regeneration of articular cartilage and subchondral bone in osteochondral defects. Therefore, SF-MMT nanocomposite hydrogel with dual-lineage bioactivity is a promising scaffold for osteochondral tissue engineering. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adhm.202200602DOI Listing
June 2022

Branched poly(ethylenimine) carbon dots-MnO nanosheets based fluorescent sensory system for sensing of malachite green in fish samples.

Food Chem 2022 Jun 18;394:133517. Epub 2022 Jun 18.

Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address:

Malachite green (MG) is an organic dye compound that is frequently used as a fungicide and antiseptic in aquaculture. However, human or animal exposure to MG causes carcinogenic, teratogenic and mutagenic effects. Herein, a novel fluorescent assay was designed for the detection of MG using manganese dioxide nanosheets (MnO NS) as an energy acceptor to quench the fluorescence of branched poly(ethylenimine) carbon dots (BPEI-CDs) via Förster resonance energy transfer. When butyrylcholinesterase is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide, MnO NS can be recovered by thiocholine to Mn, resulting in restoration of the fluorescence of BPEI-CDs. Exploiting these changes in fluorescence intensity in the above system, a fluorescence probe was successfully developed for the quantitative detection of MG. Besides, this assay was applied to fish samples, verifying the high potential for practical application of the proposed sensor for the monitoring of MG in aquatic products.
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http://dx.doi.org/10.1016/j.foodchem.2022.133517DOI Listing
June 2022

Highly Efficient Artificial Light-Harvesting Systems Constructed in an Aqueous Solution Based on Twisted Cucurbit[14]Uril.

ACS Appl Mater Interfaces 2022 Jun 24. Epub 2022 Jun 24.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China.

Relying on the supramolecular self-assembly of twisted cucurbit[14]urils (Q[14]), anthracene derivatives (ADPy), Nile red (NiR), and rhodamine B (RB), highly efficient light-harvesting systems have been successfully designed in an aqueous medium. The addition of Q[14] causes ADPy to aggregate through supramolecular self-assembly to form a supramolecular polymer ([email protected][14]) with excellent aggregation-induced fluorescence and an interesting spherical external morphology, making it a remarkable energy donor. Consequently, efficient energy-transfer processes have occurred between [email protected][14] assembly and NiR and RB, which both serve as effective energy acceptors while being loaded onto [email protected][14]. In the case of NiR, the energy-transfer efficiency is up to 72.45%, and the antenna effect is near 55.4 at a donor/acceptor ratio of 100:1, making it close to the light-harvesting systems in nature. As a result, effective water-soluble artificial light-harvesting systems are showing enormous prospective as versatile platforms for simulating photosynthesis.
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http://dx.doi.org/10.1021/acsami.2c05599DOI Listing
June 2022

Design, Synthesis and Biological Evaluation of Novel Quinoline Derivatives as Potential Anti-proliferative Agents Against PC-3 and KG-1 Cells.

Anticancer Agents Med Chem 2022 Jun 23. Epub 2022 Jun 23.

Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, China.

Background: Cancer is a major public health problem worldwide and it is the leading cause of death currently. The discovery and development of cancer therapeutic drugs has become the most urgent measure, which significantly benefited from the usage of small molecule compounds. The quinoline core possessed a vast number of biological activities that were found to be imperative.

Objective: The aim is to design, synthesize and perform biological evaluation of novel quinoline derivatives as potential anti-proliferative agents.

Methods: Quinoline as a privileged scaffold was adopted to introduce diverse effective nitrogen heterocycles through different linkers. The synthesized compounds were spectroscopically characterized and evaluated for their anti-proliferative activity using CCK8 assay. The mechanism of action was investigated by flow cytometry and the inhibitory activity against Pim-1 kinase was measured by mobility shift assay. A molecular docking analysis was performed to rationalize biochemical potency as well.

Results: The majority of these quinolines displayed potent growth inhibitory effects, among which compounds 13e, 13f and 13h were the most effective ones with GI50 values of 2.61/3.56, 4.73/4.88 and 4.68/2.98 μM, respectively. Structure-activity relationships indicated that both appropriate heterocycle at C4 position of pyridine and suitable substituent at quinoline had significant impact on improving activity. Compounds 13e and 24d exhibited moderate Pim-1 kinase inhibitory activity.

Conclusion: In this study, three series of novel molecules bearing quinoline scaffold were designed, synthesized and evaluated for their in-vitro anti-proliferative activity. The most promising candidate 13e caused cell cycle arrest in a concentration-dependent manner and further induced apoptosis, which might represent a novel anti-proliferative agent working through Pim-1 kinase inhibition to a certain extent.
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http://dx.doi.org/10.2174/1871520622666220623103957DOI Listing
June 2022

Sevoflurane suppresses hepatocellular carcinoma cell progression via circ_0001649/miR-19a-3p/SGTB axis.

Histol Histopathol 2022 Jun 24:18484. Epub 2022 Jun 24.

Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou City, Henan Province, China.

Background: Sevoflurane is a widely used anesthetic agent and is reported to play an anti-tumor action in many cancers. However, the underlying mechanisms are largely unclear.

Methods: Hepatocellular carcinoma (HCC) cells were treated with sevoflurane for 12 or 24 h. HCC cell proliferation, migration, invasion, and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay, transwell assay, and flow cytometry assay, respectively. The protein levels were determined by western blot. The expression of circular RNA (circ)_0001649, microRNA (miR)-19a-3p, and small glutamine rich tetratricopeptide repeat containing Beta (SGTB) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between miR-19a-3p and circ_0001649 or SGTB was predicted by Starbase and confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays.

Results: Sevoflurane inhibited HCC cell proliferation, migration, and invasion, but promoted apoptosis. Sevoflurane could affect the expression of circ_0001649 and knockdown of circ_0001649 reversed the effects of sevoflurane on HCC cell progression. Subsequently, miR-19a-3p was identified as a target of circ_0001649 and directly targeted SGTB. In addition, circ_0001649 suppressed the development of sevoflurane-induced HCC cells through miR-19a-3p/SGTB axis.

Conclusion: Our study demonstrated that sevoflurane inhibited HCC cell development via circ_0001649/miR-19a-3p/SGTB axis.
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http://dx.doi.org/10.14670/HH-18-484DOI Listing
June 2022

Bioinformatics Approach Predicts Candidate Targets for SARS-CoV-2 Infections to COPD Patients.

Biomed Res Int 2022 21;2022:1806427. Epub 2022 Jun 21.

Department of Pulmonary and Critical Care Medicine, Jinan University First Affiliated Hospital, Guangzhou 510630, China.

COVID-19 is still prevalent in more world regions and poses a severe threat to human health due to its high pathogenicity. The incidence of COPD patients is gradually increasing, especially in patients over 45 years old. COPD patients are susceptible to COVID-19 due to the specific lung receptor ACE2 of SARS-CoV-2. We attempt to reveal the genetic basis by analyzing the expression of common DEGs of the two diseases through bioinformatics approaches and find potential therapeutic agents based on the target genes. Thus, we search the GEO database for COVID-19 and COPD transcriptomic gene expression. We also study the enrichment of signaling regulatory pathways and hub genes for potential therapeutic treatments. There are 34 common DEGs in the two datasets. The signaling pathways are mainly enriched in intercellular junctions between virus and cytokine regulation. In the PPI network of common DEGs, we extract 5 hub genes. We find that artesunate CTD 00001840, dexverapamil MCF7 UP, and STOCK1N-35696 PC3 DOWN could be therapeutic agents for both diseases. We also analyze the regulatory network of differential genes with transcription factors and miRNAs. Therefore, we conclude that artesunate CTD 00001840, dexverapamil MCF7 UP, and STOCK1N-35696 PC3 DOWN can be therapeutic candidates in COPD combined with COVID-19.
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http://dx.doi.org/10.1155/2022/1806427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9211381PMC
June 2022

Case Report: Bilateral Biportal Endoscopic Open-Door Laminoplasty With the Use of Suture Anchors: A Technical Report and Literature Review.

Front Surg 2022 7;9:913456. Epub 2022 Jun 7.

Department of Orthopaedics, Hangzhou Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, China.

Background: Unilateral biportal endoscopy (UBE) is a newly developed technique for spine surgery. Owing to the convenience of nerve decompression and compatibility with open surgical instruments under endoscopic guidance, this technique has seen widespread global use. In this study, we first used modified UBE with suture anchor fixation for cervical laminoplasty in a 65-year-old female patient with good clinical outcomes.

Methods: We used bilateral biportal endoscopy (BBE) for cervical laminoplasty with suture anchor fixation in a patient with cervical stenosis. Under endoscopic guidance, a bilateral approach was used to make the gutter and lift the lamina door. After the lamina doors were opened, sutures were tied tightly using facia cannula and knot pusher. After confirming the solidarity of the open-door status, the drainage tube was inserted and the incisions were closed. The patient's pre- and postoperative radiological and clinical results were evaluated.

Results: Postoperative Japanese Orthopaedic Association (JOA) and Neck Disability Index (NDI) scores were improved clinically, and cervical canal was decompressed radiologically.

Conclusions: BBE laminoplasty combined with suture anchor fixation showed a favorable clinical and radiological result and appears to be a safe and effective technique for cervical stenosis.
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http://dx.doi.org/10.3389/fsurg.2022.913456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209651PMC
June 2022

Efficacy and Safety of for Treating Chronic Kidney Diseases: A Systematic Review and Meta-Analysis.

Evid Based Complement Alternat Med 2022 14;2022:2117433. Epub 2022 Jun 14.

Macau University of Science and Technology, Faculty of Chinese Medicine, Taipa 999078, Macao, China.

Objective: This meta-analysis evaluated the effects and potential harms of or its extracts Salvianolate and Tanshinone for the treatment of population with a chronic kidney disease (CKD).

Methods: We searched for the randomized clinical trials (RCTs) through databases including the Cochrane Library, PubMed, Embase, Web of Science, Current Controlled Trials, China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform (Wanfang Data), China Biology Medicine Disc (SinoMed), and Chinese Clinical Trial Registry (ChiCTR). Meta-analysis was performed with STATA 16 software after data extraction. The risk of bias was assessed with the Cochrane risk-of-bias tool (RoB 2.0), and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) framework was employed to evaluate the quality of evidence.

Result: A total of 32 studies were included involving 2264 participants. Compared to the control group, the treatment group significantly decreased serum creatinine (SCr) (SMD -0.60, 95% CI -0.79 to -0.41, < 0.0001), blood urea nitrogen (BUN) (SMD -0.66, 95% CI -0.81 to -0.50, < 0.0001), Cystatin C (CysC) (SMD -5.16, 95% CI -14.84 to 4.53, =0.297), 24 hour urine protein (24 h UPE) (SMD -0.70, 95% CI -1.21 to -0.19, =0.008), time to initiation of dialysis (Log RR 0.43, 95% CI 0.23 to 0.81, =0.0089), serum total cholesterol (TC) (SMD -0.53, 95% CI -0.88 to -0.17, =0.0042, =0.0035), plasma fibrinogen (FIB) (SMD -0.79, 95% CI -1.12 to -0.46, < 0.0001), C-reactive protein (CRP) (SMD -0.56, 95% CI -0.93 to -0.19, =0.0029); increased creatinine clearance (Ccr) (SMD 0.92, 95% CI 0.43 to 1.41, =0.0002), glomerular filtration rate (GFR) (SMD 0.56, 95% CI 0.30 to 0.83, < 0.001), effective rate (Log RR 0.30, 95% CI 0.23 to 0.37, < 0.0001), and hemoglobin (Hb) (SMD 0.42, 95% CI 0.13 to 0.71, =0.0042). Moreover, the incidences of adverse effects were similar between the two groups.

Conclusions: or its extracts Salvianolate and Tanshinone, as a complementary therapy to conventional medicine, presents potential impacts to improve kidney functions and delay the progression of CKD without obvious adverse effects. However, the certainty of the evidence and the risk of bias are suboptimal and further clinical studies are still required to determine the underlying effects.
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http://dx.doi.org/10.1155/2022/2117433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213127PMC
June 2022

Design of a Morphing Skin with Shape Memory Alloy Based on Equivalent Thermal Stress Approach.

Micromachines (Basel) 2022 Jun 13;13(6). Epub 2022 Jun 13.

China Aerodynamics Research and Development Center, Mianyang 621000, China.

Shape memory alloy (SMA) is one of the potential driving devices for morphing aircraft due to its advantages of pseudoelasticity, superelasticity, and shape memory effect. Precise and fast analysis of SMA has simultaneously become a key requirement for industrial applications. In this study, a user-defined material subroutine (UMAT) was implemented and successfully applied in a three-dimensional numerical simulation in ABAQUS based on the extended Boyd-Lagoudas model. In addition to the conventional detwinned martensite () and austenite (), twinned martensite () was also considered to model the practical transformation accurately. Then, the equivalent thermal strain approach was adopted to simplify the simulation complexity with UMAT. By resetting the thermal expansion coefficient, the thermal strain equivalent to the original phase transformation strain was generated. The approach was validated in two cases, showing consistent results with the extended Boyd-Lagoudas model and reduction in time consumption by 89.1%. Lastly, an active morphing skin integrating the single-range SMA and a stainless-steel plate was designed to realize two-way morphing. The calculated arc height variation of the skin was 3.74 mm with a relative error of 1.84% compared to the experimental result of 3.81 mm. The coupled use of UMAT and the equivalent thermal stress approach helped to reduce the challenge in modeling SMA.
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http://dx.doi.org/10.3390/mi13060939DOI Listing
June 2022

Wafer-Level Vacuum-Packaged Electric Field Microsensor: Structure Design, Theoretical Model, Microfabrication, and Characterization.

Micromachines (Basel) 2022 Jun 11;13(6). Epub 2022 Jun 11.

State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.

This paper proposes a novel wafer-level vacuum packaged electric field microsensor (EFM) featuring a high quality factor, low driving voltage, low noise, and low power consumption. The silicon-on-insulator (SOI) conductive handle layer was innovatively used as the sensing channel to transmit the external electric field to the surface of the sensitive structure, and the vacuum packaging was realized through anodic bonding between the SOI and glass-on-silicon (GOS). The fabrication process was designed and successfully realized, featured with a simplified process and highly efficient batch manufacturing, and the final chip size was only 5 × 5 mm. A theoretical model for the packaged device was set up. The influence of key parameters in the packaging structure on the output characteristics of the microsensor was analyzed on the basis of the proposed model. Experiments were conducted on the wafer-level vacuum-packaged EFM to characterize its performance. Experimental results show that, under the condition of applying 5 V DC driving voltage, the required AC driving voltage of the sensor was only 0.05 V, and the feedthrough was only 4.2 mV. The quality factor was higher than 5000 and was maintained with no drop in the 50-day test. The vacuum in the chamber of the sensor was about 10 Pa. A sensitivity of 0.16 mV/(kV/m) was achieved within the electrostatic field range of 0-50 kV/m. The linearity of the microsensor was 1.62%, and the uncertainty was 4.42%.
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http://dx.doi.org/10.3390/mi13060928DOI Listing
June 2022

Study on the Conventional Performance and Microscopic Properties of PPA/SBS-Modified Bio-Mixed Asphalt.

Materials (Basel) 2022 Jun 9;15(12). Epub 2022 Jun 9.

School of Civil Engineering and Architecture, Guangxi University, Nanning 530004, China.

To promote the construction of environmentally friendly, sustainable pavements and solve the impact of the scarcity of asphalt resources on highway development, bio-mixed asphalt (BMA) modified by SBS and polyphosphoric acid (PPA) was prepared, and the influence of the ratio of bio-asphalt (BA) replacing petroleum asphalt on different PPA/SBS blending schemes was explored through conventional property tests. According to each PPA/SBS blending scheme, the optimal replacement ratio of bio-asphalt was optimized, and the microstructure and distribution morphology of different PPA/SBS-modified BMA were evaluated. Conventional property test results show that with the same PPA/SBS content, the replacement ratio of bio-asphalt has a significant impact on the conventional performance of composite-modified asphalt, but the appropriate replacement ratio of bio-asphalt can improve the storage stability and conventional performance of composite-modified asphalt; in micromorphological analysis, it was found that the number of bee-like structures on the surface of the modified BMA decreased significantly, which indicated that the molecular heterogeneity of various components in the asphalt was reduced. In addition, bio-asphalt changed the particle morphology and improved the dispersity of SBS in asphalt. The composite-modified BMA had a lower SBS content, but its conventional performance was still excellent-so it has significant application prospects in road engineering.
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http://dx.doi.org/10.3390/ma15124101DOI Listing
June 2022

How to Evaluate Investment Efficiency of Environmental Pollution Control: Evidence from China.

Int J Environ Res Public Health 2022 Jun 13;19(12). Epub 2022 Jun 13.

School of Public Administration, Sichuan University, Chengdu 610065, China.

Clarifying the efficiency of investment in environmental pollution control is conducive to better control of environmental pollution. Based on panel data of 30 provinces and cities in China from 2008 to 2017, this study combines the three-stage super-efficient SBM-DEA model and the Global-Malmquist-Luenberger index to measure the efficiency of investment in environmental pollution control in China and analyze regional differences. The results show that: First, the investment efficiency of environmental pollution control in China shows a rising trend year by year, but there are significant differences among provinces and regions; the presence of random factors and environmental variables makes the control efficiency underestimated. Second, excluding the effects of both, the national investment efficiency of environmental pollution control has improved significantly, but still has not reached the optimal effect; the gap between provinces and regions has narrowed while the investment efficiency of environmental pollution control has improved, and there is still an unbalanced situation. Third, the main driver of the year-on-year improvement in China's environmental pollution control efficiency is technological progress; compared with northeastern China, technological progress has a more significant role in promoting eastern, central, and western China. Finally, based on the results, this paper focuses on making suggestions to promote environmental pollution control in China in terms of making regional cooperation, making good environmental protection investment and strengthening environmental protection technology research and development.
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http://dx.doi.org/10.3390/ijerph19127252DOI Listing
June 2022

Comparisons between needle puncture and chondroitinase ABC to induce intervertebral disc degeneration in rabbits.

Eur Spine J 2022 Jun 23. Epub 2022 Jun 23.

Department of Spine Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, China.

Purpose: This study aimed to compare the effect of needle puncture and chondroitinase ABC (ChABC) injection on inducing intervertebral disc (IVD) degeneration (IVDD) in rabbits.

Methods: Sixteen New Zealand white rabbits were used in this study. Briefly, the rabbits were divided into four groups. In the annulus fibrosis (AF) needle puncture group, a 16-G needle was used to puncture the L5-6 and L6-7 IVDs, while in the sham group, these IVDs were not punctured. In the ChABC group, 30 μL 0.5 Unit/mL ChABC was injected into L5-6 and L6-7 IVDs using a 26-G needle, while in the vehicle group, these IVDs were injected with 30 μL phosphate-buffered saline (PBS). X-ray and MRI scans were performed at the 4th, 12th and 16th weeks postoperatively. Histological, immunohistochemical and biochemical analyses were performed at the 16th week postoperatively.

Results: Both needle puncture and ChABC successfully established IVDD in rabbits at 4th, 12th and 16th weeks, confirmed by X-ray and MRI scan. The progression of IVDD went in a time-dependent manner. The IVDD in the ChABC group was less severe than in the needle puncture group throughout the study. Aggrecan and type II collagen significantly decreased, while tumor necrosis factor-α and superoxide dismutase 2 increased in the needle puncture and ChABC groups, compared with the sham and PBS groups.

Conclusions: Both AF needle puncture and ChABC injection can successfully induce IVDD in rabbits. Compared with ChABC injection, AF needle puncture can induce more severe IVDD.
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http://dx.doi.org/10.1007/s00586-022-07287-8DOI Listing
June 2022

Shanghai expert consensus on clinical protocol for traditional Chinese medicine treatment of COVID-19 among the elderly population (second edition).

J Integr Med 2022 Jun 8. Epub 2022 Jun 8.

Research Center for Traditional Chinese Medicine, Shanghai Institute of Infectious Diseases and Biosecurity, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

This document is the revised edition of the previously issued Shanghai Expert Consensus on Clinical Protocol for Traditional Chinese Medicine Treatment of COVID-19 among the Elderly Population. Based on the clinical experience and the Protocol for Diagnosis and Treatment of COVID-19 (Trial 9th Edition), this revised edition provides treatment approaches and recommendations to proactively cope with Omicron variant and increase the therapeutic efficacy for coronavirus disease 2019 among the elderly population in Shanghai, China.
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http://dx.doi.org/10.1016/j.joim.2022.06.001DOI Listing
June 2022

Characterization of Plant Accumulation of Pharmaceuticals from Soils with Their Concentration in Soil Pore Water.

Environ Sci Technol 2022 Jun 23. Epub 2022 Jun 23.

Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824, United States.

Predicting plant uptake of pharmaceuticals from soils is very challenging because many pharmaceuticals are ionizable compounds, which experience highly variable sorption/desorption and transformation processes in soils. This study aimed to elucidate how the equilibrium between sorbed and dissolved phases influences radish uptake of 15 pharmaceuticals from three soils with different properties. After 30 days of uptake, the accumulation of acetaminophen, carbamazepine, lamotrigine, carbadox, trimethoprim, and triclosan in radish ranked as Riddles > Capac > Spinks soil. In contrast, radish accumulation of caffeine, lincomycin, monensin, tylosin, sulfadiazine, and sulfamethoxazole exhibited the opposite order of Riddles < Capac < Spinks soil. Oxytetracycline and estrone demonstrated similar accumulation in radish grown in the three soils. Accumulation of pharmaceuticals in radish demonstrated no apparent relation with their concentration in soils. However, we identified strong positive correlation between pharmaceutical accumulation in radish and their corresponding concentration in soil pore water. These results reveal that pharmaceutical in soil pore water is the dominant fraction bioavailable to plant uptake. Relatively constant root concentration factors (RCFs) on the basis of pharmaceutical concentration in soil pore water, compared to the highly variable RCFs derived from soils, suggest that pore water-based RCF is superior for describing pharmaceutical accumulation in plants grown in soils. We recommend that pharmaceuticals in soil pore water should be evaluated and included in modeling their uptake by plants.
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http://dx.doi.org/10.1021/acs.est.2c00303DOI Listing
June 2022

Simultaneous fluorescence imaging of Golgi O and Golgi HO in mice with hypertension.

Biosens Bioelectron 2022 Jun 17;213:114480. Epub 2022 Jun 17.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Minis-try of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University, Jinan, 250014, People's Republic of China. Electronic address:

Hypertensive cardiovascular disease is a persistent threat to public health. Elucidating the pathogenesis of hypertension is expected to provide more highly targeted therapies for patients. To date, reactive oxygen species (ROS) induced endothelial nitric oxide synthase (eNOS) uncoupling are generally considered to be common phenomena in hypertension. However, the critical factor contribute to persistent eNOS uncoupling remains poorly understood. Herein, we established a fluorescence probe, Gol, for the multicolored and simultaneous detection of Golgi O and HO in situ. We successfully detected increases in Golgi ROS levels in hypertensive mice and evaluated the pharmaceutical effects of various antihypertensive drugs. More importantly, we identified the ROS post-transcriptional modification sites on dihydrofolate reductase (DHFR). Altogether, we propose a novel therapeutic target for hypertension, which will promote the development of new antihypertensive drugs, and also developed an ideal fluorescence probe to study in situ Golgi O and HO changes in various biochemical processes.
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http://dx.doi.org/10.1016/j.bios.2022.114480DOI Listing
June 2022

Cancer bone metastases and nanotechnology-based treatment strategies.

Expert Opin Drug Deliv 2022 Jun 23. Epub 2022 Jun 23.

Department of Orthopedic, Zhuhai People's Hospital (Zhuhai hospital affiliated with Jinan University), Zhuhai, Guangdong, China.

Introduction: Bone metastases have gradually been recognized as common metastases that affect patient quality of life and survival due to the increased incidence of primary tumors. However, there is still a lack of effective clinical treatment methods for bone metastases because of their particularity and complexity. Nanomedicine provides a new strategy for the treatment of bone metastases and shows great therapeutic potential. Thus, it is important to review the latest nanomedicine treatments for bone metastases.

Areas Covered: This review introduces the mechanistic relationships of bone metastases and summarizes nanotechnology-based treatments of bone metastases according to targeting strategies.

Expert Opinion: As we start to understand the mechanisms that enable bone metastases, we can better develop nanomedicine treatments. However, many of the mechanisms behind bone metastasis remain unclear. The application of nanomedicine shows promising anti-bone metastasis efficacy and helps to explore the pathogenesis of bone metastases. The optimized construction of nanomedicine according to bone metastatic properties is crucial to ensure the desired anti-bone metastasis efficacy and good biosafety. Therefore, the transition from bench to bedside still requires continued exploration.
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http://dx.doi.org/10.1080/17425247.2022.2093856DOI Listing
June 2022

Modulation of genes expression in aged rat liver, brain, and kidney by caloric restriction analyzed via single-nucleus/cell RNA sequencing.

Autophagy 2022 Jun 23:1-10. Epub 2022 Jun 23.

Department of Geriatrics, Shenzhen People's Hospital (The Second Clinical Medical College of Jinan University; the First Affiliated Hospital of Southern University of Science and Technology), Shenzhen, Guangdong, China.

Dysregulation of macroautophagy/autophagy has been closely implicated in aging. Caloric restriction (CR) is an effective intervention of aging partially via activation of autophagy. Recently, a high-throughput single-cell RNA-seq technique has been employed to detect the comprehensive transcriptomes of individual cells. However, the transcriptional networks of (autophagy related) genes in the aging process and the modulation of genes expression by CR at the single-cell level have not been elucidated. Here, by performing data analysis of single nucleus/cells RNA sequencing in rats undergoing aging and the modulation by CR, we demonstrate that the transcription patterns of genes in different cell types of rat liver, brain, and kidney are highly heterogeneous. Importantly, CR reversed aging-induced changes of multiple genes across different cell types in the brain, liver, and kidney. In summary, our results, for the first time, provide comprehensive information on gene expression in specific cell types of different organs in a mammal during aging and give novel insight into the protective role of autophagy and CR in aging at the single-cell resolution. genes: autophagy-related genes; : autophagy related 5; : autophagy related 7; CR: caloric restriction; DEATG: differentially expressed autophagy-related; NAFLD: nonalcoholic fatty liver disease; ScRNA-seq: single-cell RNA sequencing.
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http://dx.doi.org/10.1080/15548627.2022.2091903DOI Listing
June 2022

Epigenetic Signatures of Centrosomes Are Novel Targets in Cancer Diagnosis: Insights from an Analysis of the Cancer Genome Atlas.

Epigenomes 2022 Jun 2;6(2). Epub 2022 Jun 2.

Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

The centrosome plays a central role for cellular signaling and is critical for several fundamental cellular processes in human cells. Centrosome abnormalities have been linked to multiple solid tumors and hematological malignancies. We sought to explore the potential role of the DNA methylation, a critical epigenetic modification, of centrosome-related genes in different cancers. The 450K array DNA methylation data and RNA-seq data were downloaded for ~4000 tumor samples and ~500 normal controls from The Cancer Genome Atlas (TCGA) project, covering 11 major cancer types. Cancers with more than 30 normal controls were retained for analysis. Differentially modified CpGs of centrosome genes were identified, and cancer-specific epigenetic models were developed using a machine-learning algorithm for each cancer type. The association between the methylation level of differential CpGs and the corresponding gene expression, as well as the co-localization of the differential CpGs and -regulatory elements were evaluated. In total, 2761 CpGs located on 160 centrosome genes for 6 cancers were included in the analysis. Cancer-specific models demonstrated a high accuracy in terms of the area under the receiver operating characteristic (ROC) curve (AUC > 0.9) in five cancers and showed tissue specificity. This study enhanced our understanding of the epigenetic mechanisms underlying the DNA methylation of centrosome-related genes in cancers, and showed the potential of these epigenetic modifications as novel cancer biomarkers.
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http://dx.doi.org/10.3390/epigenomes6020014DOI Listing
June 2022

Impact of allograft types on outcomes after pediatric liver transplantation due to biliary atresia.

Pediatr Transplant 2022 Jun 23:e14342. Epub 2022 Jun 23.

Department of Pediatric transplantation, Organ Transplantation Center, Tianjin First Central Hospital, Tianjin, China.

Background: Several surgical strategies, including split donor transplantation and living donor transplantation, have been used to increase the donor liver pool. This report focuses on the effects of whole, split, and LDLT on recipient outcomes.

Methods: We retrospectively analyzed the records of all patients with biliary atresia at Tianjin First Central Hospital between April 2013 and December 2019.

Results: A total of 882 patients were included and divided into three groups by graft type, with 198 in the whole-liver-transplantation group, 78 in the split liver transplantation group, and 606 in the LDLT group. The median follow-up time was 39 months, patient survival rates of three groups were 94.4%, 88.5%, and 95.0%, respectively, and graft survival rates were 90.2%, 83.3%, and 94.7%, respectively. We divided the split liver transplantation group into two subgroups according to the donor's age, and patient survival rates exhibited a significant difference only in the group whose donor age was over 45 years. The postoperative complication rates were significantly higher with respect to hepatic artery thrombosis, portal stenosis, and AR; and lower in hepatic venous stenosis, PTLDs, CMV virus, and EBV infection in the WLT group. Our multivariate model showed that donor age ≥45 years, RBC transfusion, pneumonia, and HAT were the independent predictors of allograft loss.

Conclusions: The survival of split liver transplantation group was slightly lower. The types of complications are different from different graft types. Therefore, postoperative monitoring and treatment need to be adjusted according to the different graft types used.
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http://dx.doi.org/10.1111/petr.14342DOI Listing
June 2022

Interferon-mediated repression of miR-324-5p potentiates necroptosis to facilitate antiviral defense.

EMBO Rep 2022 Jun 23:e54438. Epub 2022 Jun 23.

Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, Soochow University, Suzhou, China.

Mixed lineage kinase domain-like protein (MLKL) is the terminal effector of necroptosis, a form of regulated necrosis. Optimal activation of necroptosis, which eliminates infected cells, is critical for antiviral host defense. MicroRNAs (miRNAs) regulate the expression of genes involved in various biological and pathological processes. However, the roles of miRNAs in necroptosis-associated host defense remain largely unknown. We screened a library of miRNAs and identified miR-324-5p as the most effective suppressor of necroptosis. MiR-324-5p downregulates human MLKL expression by specifically targeting the 3'UTR in a seed region-independent manner. In response to interferons (IFNs), miR-324-5p is downregulated via the JAK/STAT signaling pathway, which removes the posttranscriptional suppression of MLKL mRNA and facilitates the activation of necroptosis. In influenza A virus (IAV)-infected human primary macrophages, IFNs are induced, leading to the downregulation of miR-324-5p. MiR-324-5p overexpression attenuates IAV-associated necroptosis and enhances viral replication, whereas deletion of miR-324-5p potentiates necroptosis and suppresses viral replication. Hence, miR-324-5p negatively regulates necroptosis by manipulating MLKL expression, and its downregulation by IFNs orchestrates optimal activation of necroptosis in host antiviral defense.
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http://dx.doi.org/10.15252/embr.202154438DOI Listing
June 2022

Exploration of the Effect and Potential Mechanism of Echinacoside Against Endometrial Cancer Based on Network Pharmacology and in vitro Experimental Verification.

Drug Des Devel Ther 2022 16;16:1847-1863. Epub 2022 Jun 16.

Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.

Background: Endometrial cancer (EC) is one of the most common gynecological malignancies, especially in postmenopausal women. Echinacoside (ECH) is a major natural bioactive ingredient derived from Cistanches Herba and Echinacea that has a variety of pharmacological effects. However, the efficacy and the mechanism of ECH against EC have not been elucidated yet.

Purpose: A compound-target-disease network was constructed to explore the potential targets and mechanism of ECH against EC. Molecular docking and in vitro experiments further verified the effect of ECH against EC.

Methods: The potential targets of ECH against EC were retrieved from multiple public databases. Then, the protein-protein interaction (PPI) network was constructed to screen hub targets. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to discover the potential mechanism. Molecular docking was utilized to verify the binding affinity between hub targets and ECH. Finally, in vitro experiments were conducted to demonstrate the anti-EC effect of ECH.

Results: A total of 110 genes were identified as potential targets of ECH against EC. The GO enrichment analysis found that targets were primarily related to oxygen species, apoptosis, and other physiological processes. KEGG pathway analysis showed that PI3K/Akt signaling pathways might play an important role in ECH against EC. Molecular docking indicated that ECH had a significant binding ability with the EGFR, AKT1, ESR1, CASP3, HSP90AA1and MMP9 targets. Results from in vitro experiments revealed that ECH induced apoptosis of Ishikawa and HEC-1-B cells by promoting the arrest of the G2M phase, increasing ROS levels, and decreasing mitochondrial membrane potential (MMP) levels. Furthermore, treatment of ECH significantly reduced the expression levels of PI3K and p-AKT, and the combination of the PI3K inhibitor (LY294002) further enhanced the effects of ECH against EC. The findings suggested that ECH exerted an inhibitory effect on EC cells by inhibiting the PI3K/AKT pathway.

Conclusion: Based on network pharmacology, molecular docking technology and in vitro experiments, we comprehensively clarified the anti-EC efficacy of ECH through multiple targets and signal pathways. Furthermore, we provided a novel idea of Traditional Chinese medicine (TCM) against EC.
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http://dx.doi.org/10.2147/DDDT.S361955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208491PMC
June 2022

Non-Wilms' renal tumors in children: experience with 139 cases treated at a single center.

BMC Urol 2022 Jun 22;22(1):89. Epub 2022 Jun 22.

Department of Urology, National Children's Medical Center, Beijing Children's Hospital of Capital Medical University, No. 56 Nanlishi St, Xicheng District, Beijing, 100045, China.

Background: Pediatric non-Wilms renal tumors (NWRTs), which comprise a small proportion of renal tumors, are a heterogeneous group of neoplasms with variable malignant potential, mortality, and response to treatment. We performed this study to determine the clinical characteristics, management and prognosis of children with Pediatric NWRTs.

Methods: Medical records of all patients (n = 139) treated for NWRTs over a 12-year period (2008.01-2019.10) at a single center were reviewed retrospectively.

Results: The histopathological groups of NWRTs included malignant rhabdoid tumor of the kidney (MRTK) (n: 30, 21.6%), renal cell cancer (RCC) (n: 26,18.7%), clear cell sarcoma of the kidney (CCSK) (n: 24,17.3%), congenital mesoblastic nephroma (CMN) (n: 21,15.1%), cystic nephroma (CN) (n: 16,11.5%), metanephric tumors (n: 12, 8.6%), renal angiomyoliporma (RAML) (n: 3, 2.2%), renal primitive neuroectodermal tumor (n: 2, 1.4%), renal hemangioma (n: 2, 1.4%), inflammatory myofibroblastic tumor (n: 2, 1.4%), ossifying renal tumor of infancy (ORTI) (n: 1, 0.7%). The distribution of all malignant NWRTs, including MRTK, CCSK, RCC and PNET, according to stage was as follows: stages I (n = 26), II (n = 16), III (n = 29), and IV (n = 11). The summary table shows the treatment offered to children with NWRTs. A total of 123 children were followed up for an average of 42 months. Sixteen children were lost to follow-up. Tumor-free survival was observed in 94 children. One patient who suffered from RCC is currently receiving targeted therapy and survives with the tumor. Twenty-eight children (22.8%) died.

Conclusions: Pediatric NWRTs comprise 19.1% of all renal tumors in our single center. Most NWRTs can readily be distinguished using a range of immunohistochemical markers. Molecular genetic profiling has allowed much progress in the understanding of this group of tumors, making diagnosis and classification less difficult. The mainstay treatment of malignant NWRTs, including MRTK, CCSK, RCC and PNET, is comprehensive treatment. The mainstay treatment of benign NWRTs, including RAML, CN, ORTI, CMN, metanephric tumors, and renal hemangioma, is surgical resection alone and when the tumor diameter is smaller than 7 cm and the tumor locates in one pole, NSS can be performed.
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http://dx.doi.org/10.1186/s12894-022-01042-3DOI Listing
June 2022

Mussel-Inspired Surface Coating to Stabilize and Functionalize Supramolecular -Aggregate Nanotubes Composed of Amphiphilic Cyanine Dyes.

Langmuir 2022 Jun 22. Epub 2022 Jun 22.

School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, 2001 Longxiang Avenue, Shenzhen 518172 Guangdong, China.

We report a mussel-inspired strategy of polydopamine (PDA) coating to stabilize and functionalize -aggregate nanotubes (NTs) formed by supramolecular self-assembly of an amphiphilic cyanine dye called C8S3 in aqueous media. Optimization of the coating condition by changing the incubation time in a slightly basic media of dopamine with different concentrations leads to conformal wrapping of the PDA layer with controllable thickness on the surface of the NTs. Compared to noncoated pristine C8S3 NTs, these PDA-coated NTs show enhanced stability against dilution, heating, and photobleaching. Moreover, the PDA layer wrapping around the NTs serves as an adhesive for the adsorption of a variety of metal ions and electroless deposition of the metal nanoparticles. Such stabilized and functionalized NT composites may offer a robust synthetic -aggregate system to mimic the structure and function of light-harvesting complexes and reaction centers in photosynthetic systems.
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http://dx.doi.org/10.1021/acs.langmuir.2c01136DOI Listing
June 2022

Construction and validation of N6-methyladenosine long non-coding RNAs signature of prognostic value for early biochemical recurrence of prostate cancer.

J Cancer Res Clin Oncol 2022 Jun 22. Epub 2022 Jun 22.

Department of Urology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, No. 1 DaHua Road, Dong Dan, Beijing, 100730, China.

Purpose: Early biochemical recurrence (eBCR) indicated a high risk for potential recurrence and metastasis in prostate cancer. The N6-methyladenosine (m6A) methylation modification played an important role in prostate cancer progression. This study aimed to develop a m6A lncRNA signature to accurately predict eBCR in prostate cancer.

Methods: Pearson correlation analysis was first conducted to explore m6A lncRNAs and univariate Cox regression analysis was further performed to identify m6A lncRNAs of prognostic roles for predicting eBCR in prostate cancer. The m6A lncRNA signature was constructed by least absolute shrinkage and selection operator analysis (LASSO) in training cohort and further validated in test cohort. Furthermore, half maximal inhibitory concentration (IC50) values were utilized to explore potential effective drugs for high-risk group in this study.

Results: Five hundred and thirty-eighth m6A lncRNAs were searched out through Pearson correlation analysis and 25 out of 538 m6A lncRNAs were identified to pose prediction roles for eBCR in prostate cancers. An m6A lncRNA signature including 5 lncRNAs was successfully built in training cohort. The high-risk group derived from m6A lncRNA signature could efficiently predict eBCR occurrence in both training (p < 0.001) and test cohort (p = 0.002). ROC analysis also confirmed that lncRNA signature in this study posed more accurate prediction roles for eBCR occurrence when compared with PSA, TNM stages and Gleason scores. Drug sensitivity analysis further discovered that various drugs could be potentially utilized to treat high-risk samples in this study.

Conclusions: The m6A lncRNA signature in this study could be utilized to efficiently predict eBCR occurrence, various clinical characteristic and immune microenvironment for prostate cancer.
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http://dx.doi.org/10.1007/s00432-022-04040-yDOI Listing
June 2022

Promoting optoelectronic properties of CsAgBiBr nanocrystals by formation of heterostructures with BiOCl nanosheets.

Chem Commun (Camb) 2022 Jun 22. Epub 2022 Jun 22.

Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China.

Lead halide perovskite nanocrystals and their heterostructures have achieved substantial advances in optoelectronics; however, their inherent material instability and lead toxicity have driven research on alternative material systems. Herein, solution-processable heterostructures composed of lead-free double perovskite CsAgBiBr nanocrystals and BiOCl nanosheets were prepared through a colloidal synthesis method. Defect states were present in BiOCl and benefited carrier generation, recombination and transport in CsAgBiBr. As a result, the light emission of the CsAgBiBr nanocrystals was greatly enhanced at low temperatures, and the photodetector based on the CsAgBiBr/BiOCl heterostructure exhibited a much improved on-off ratio compared to the device based on CsAgBiBr alone. Our work highlights the complex nature and impact of two-dimensional heterostructure assembly on the optoelectronic properties of lead-free double perovskites and demonstrates their great potential toward environmentally friendly optoelectronic devices.
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http://dx.doi.org/10.1039/d2cc01684bDOI Listing
June 2022
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