Publications by authors named "Wei Zeng"

534 Publications

Detection of Oesophageal Fistula by Radionuclide Salivagram SPECT/CT.

Front Oncol 2021 24;11:612122. Epub 2021 Aug 24.

Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, China.

Purpose: Videofluoroscopic swallowing study (VFSS) is currently the most widely used clinical examination method for diagnosis of oesophageal fistula, but it has many limitations. Therefore, we evaluated radionuclide salivagram single-photon emission computed tomography (SPECT/CT) as a new method of oesophageal fistula diagnosis.

Methods: We retrospectively evaluated the data of 11 patients (10 men and 1 woman, aged 41 to 70 years, with an average age of 58.6 years) who had clinically suspected oesophageal fistula from January 2019 to October 2020. They underwent radionuclide salivagram SPECT/CT and VFSS examinations, and we analysed and compared the results of the two examinations.

Results: A total of 11 patients were included in this study. Ten underwent both salivagram and VFSS examinations. One patient was unable to swallow the contrast agent; therefore, only salivagram was performed, and we excluded this patient from the VFSS analysis. A total of 11 patients underwent salivagram examinations, of which 6 were positive and 5 were negative. A total of 10 patients were tested by VFSS, of which 6 results were positive and 4 were negative.

Conclusion: Radionuclide salivagram SPECT/CT and VFSS are complementary, which can greatly improve the clinical diagnosis and prognosis of oesophageal fistula. When the patient cannot perform the VFSS, or the clinical symptoms are inconsistent with the VFSS imaging findings, the salivagram is an ideal test method.
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http://dx.doi.org/10.3389/fonc.2021.612122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421790PMC
August 2021

Quercetin as an antiviral agent inhibits the Pseudorabies virus in vitro and in vivo.

Virus Res 2021 Sep 4:198556. Epub 2021 Sep 4.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430000, China. Electronic address:

Pseudorabies virus (PRV) accounts for a critical swine disease incurring economic losses worldwide. Several PRV vaccines are commercially available but these vaccines are effective against only certain prevalent PRV strains in China. To prevent PRV-induced latent infection and decrease the pathogenicity, novel anti-PRV drugs are required to prevent PRV infection. Natural products show exceptional structural diversity representing an important source for developing novel therapeutic agents. Quercetin is a flavonoid with anti-oxidant, anti-cancer, anti-bacterial and anti-viral activities. This study involved quercetin for studying the anti-PRV function in vitro and in vivo. Quercetin was found to significantly decrease the PRV virulent strain HNX at a half-maximal inhibitory concentration (IC) of 2.618 μM and selectivity index 229. This anti-PRV activity of quercetin was found to be dose-dependent. Furthermore, quercetin also inhibited a wide the infections by a spectrum of PRV strains like HNX, Ea, Bartha and Fa strain. These virucidal effects of quercetin suggest the interaction between these molecules and viral particles, and quercetin is responsible for inhibiting the adsorption of PRV infections. The silico assays suggesting that quercetin might interact with the gD-protein on the surface of the PRV important for viral infection. Additional, the quercetin plantar injection protected the mice from the lethal challenge, decreasing the PRV-infected mice's brain viral loads and mortality. These results provides a anti-PRV strategy and contribute to drug discovery and development.
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http://dx.doi.org/10.1016/j.virusres.2021.198556DOI Listing
September 2021

MicroRNA-206 relieves irradiation-induced neuroinflammation by regulating connexin 43.

Exp Ther Med 2021 Oct 16;22(4):1186. Epub 2021 Aug 16.

Department of Radiology, Affiliated Hospital of Jianghan University, The Sixth Hospital of Wuhan City, Wuhan, Hubei 430019, P.R. China.

Radiation therapy has been widely used for the treatment of various types of cancer; however, it may cause neuroinflammation during the pathological process of the disease. Astrocytes, the most abundant cell type in the central nervous system, have been confirmed to play vital roles in various diseases. Connexin (Cx)43, the main Cx type in astrocytes, which has been identified as a direct target gene of microRNA (miR)-206, was found to be involved in diseases pathologies in regions with astrocytes. The aim of the present study was to investigate the mechanism through which γ-radiation may cause astrocyte neuroinflammation and determine the specific mechanism underlying the effects of miR-206 in irradiation-induced HA-1800 cells. A dual-luciferase reporter system was used to predict and verify the target binding site between Cx43 and miR-206. HA-1800 cell viability and apoptosis were determined using a MTT assay and flow cytometry, respectively. In addition, the HA-1800 cells were induced by γ-radiation, then the protein and mRNA expression levels of Cx43, miR-206 and cleaved-caspase-3 were determined using western blot and reverse transcription-quantitative PCR analyses, respectively. ELISA was also performed to evaluate the concentrations of different inflammatory cytokines (TNF-α, IL-β, IL-6 and IFN-γ). The dual-luciferase reporter system indicated that Cx43 was a direct target of miR-206. miR-206 mimics increased the expression level of miR-206 in the astrocytes. Irradiation suppressed cell proliferation, increased apoptotic cells and enhanced cleaved-caspase-3 expression and inflammatory cytokines secretion in astrocytes. Furthermore, miR-206 was found to be downregulated and its expression was inversely associated with that of Cx43 in γ-radiation-induced astrocytes. Overexpression of miR-206 enhanced miR-206 and suppressed Cx43 expression, while Cx43 was upregulated in HA-1800 cells transfected with miR-206 mimic + Cx43-plasmid. However, the expression level of miR-206 was not significantly different in the Cx43-plasmid transfected group. In addition, it was found that miR-206 mimics relieved irradiation-induced neuroinflammation, which was confirmed by increased cell viability, and reduced cell apoptosis and cleaved caspase-3 protein expression, as well as decreased inflammatory cytokine secretion. Furthermore, all the effects of miR-206 mimics on γ-radiation-induced astrocytes were reversed by Cx43-plasmid. In summary, the results of the present study indicated that miR-206 may relieve irradiation-induced neural damage by regulating Cx43, which may provide a novel research direction and a potential therapeutic target for the clinical treatment of inflammation-associated neuronal injury following irradiation.
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http://dx.doi.org/10.3892/etm.2021.10620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406811PMC
October 2021

Identification of partial denitrification granulation enhanced by low C/N ratio in the aspect of metabolomics and quorum sensing.

Chemosphere 2021 Aug 18;286(Pt 3):131895. Epub 2021 Aug 18.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing, 100124, China.

Partial denitrification granular sludge (PDGS) and denitrification granular sludge (DGS) play an important role in nitrogen removal from wastewater. However, the inherent cause of aggregation capacity related to the ratio of COD to nitrogen (COD/N) is still unclear. In this study, metabolomics analysis was combined with microbiological analyses, granular performance and extracellular polymeric substances (EPS) structure to explore the granulation mechanism at different influent COD/N ratios. The results showed that the higher COD/N ratio selectively enhanced the gluconeogenesis pathway, purine and pyrimidine metabolism pathway, resulting in more extracellular polysaccharide (PS) excretion and floc sludge. The absence of carbon source weakened tricarboxylic acid cycle (TCA) reaction, resulting in NAD and ADP decrease, nitrite accumulation and change of microbial community structure. The amino acids biosynthesis pathway was enhanced under low COD/N ratio, which promoted the hydrophobicity of EPS. PDGS had stronger Acyl-homoserine lactones (AHLs)-based quorum sensing (QS) than DGS during the operational period. CO8-HSL, C8-HSL and C6-HSL, as the main form of AHLs, played a dominating role in DGS and PDGS. Batch tests illustrated that adding AHLs obviously improved the synthesis of the amino acids, threonine (Thr), tryptophan (Trp), methionine (Met) and glycine (Gly). Dosing AHLs regulated PS synthesis only at a high COD/N ratio. The glucose-6P, glycerate-3p and UDP-Glc were up-regulated only in DSG, which increased the hydrophilic groups in EPS. The results not only provided the new insights into the metabolism of denitrifying granular sludge, but also indicated the application potential of the technologies regarding start-up and operation of granule sludge.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131895DOI Listing
August 2021

Deletion of the crp gene affects the virulence and the activation of the NF-κB and MAPK signaling pathways in PK-15 and iPAM cells derived from G. parasuis serovar 5.

Vet Microbiol 2021 Oct 5;261:109198. Epub 2021 Aug 5.

State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. Electronic address:

Glaesserella parasuis can cause serious systemic disease (Glasser's disease) that is characterized by fibrinous polyserositis, polyarthritis and meningitis. cAMP receptor protein (CRP) is among the well studied global regulator proteins which could modulate the virulence of many pathogenic bacteria. Our previous study showed that the crp gene was involved in the regulation of growth rate, biofilm formation, stress tolerance, serum resistance, and iron utilization in G. parasuis. However, whether the crp gene could regulate the virulence of G. parasuis has not been analyzed previously. In this study, it was observed that the crp gene in G. parasuis serovar 5 (HPS5) was involved in regulating the adhesion and invasion abilities on iPAM cells, and the mRNA expression of various virulence-related factors. It also possessed the ability to induce the mRNA expression of pro-inflammatory cytokines (IL-1α, IL-1β, IL-6, IL-8 and TNF-α), promoted the activation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways in porcine kidney epithelial (PK-15) and immortalized swine pulmonary alveolar macrophage (iPAM) cells, and contributed to the pathogenicity and organs colonization in mice. As compared with the wild type, both the expression of virulence-related factors in the crp mutant strain and its ability to induce the mRNA expression of pro-inflammatory cytokines, as well as the expression of phospho-p65 and phospho-p38 in PK-15 and iPAM cells was reduced significantly. Furthermore, it also found that the virulence of crp mutant was significantly reduced as compared with the wild type. However, the abilities of adherence and invasion on iPAM cell of Δcrp strain was noted to be significantly enhanced as compared with the wild type. These results suggested that the crp gene deletion could effectively attenuate the virulence of G. parasuis, and crp gene may act as an important potential target for the formulation of a novel vaccine against G. parasuis.
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http://dx.doi.org/10.1016/j.vetmic.2021.109198DOI Listing
October 2021

Visible-Light-Catalyzed in Situ Denitrogenative Sulfonylation of Sulfonylhydrazones.

Org Lett 2021 Sep 18;23(17):6784-6788. Epub 2021 Aug 18.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China.

A photocatalyzed denitrogenative sulfonylation of -arylsulfonyl hydrazones has been developed. This transformation provides a low-carbon strategy to assemble arylalkyl sulfones in a stepwise denitrogenation/sulfonylation manner.
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http://dx.doi.org/10.1021/acs.orglett.1c02369DOI Listing
September 2021

Roles of HDAC2, eIF5, and eIF6 in Lung Cancer Tumorigenesis.

Curr Med Sci 2021 Aug 17;41(4):764-769. Epub 2021 Aug 17.

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China.

Objective: The expression levels of histone deacetylase 2 (HDAC2), eukaryotic initiation factor 5 (eIF5), and eukaryotic initiation factor 6 (eIF6), and relationship between HDAC2 and eIF5 or eIF6 in lung cancer tissues were investigated, in order to charify the relationship between HDAC2 and the prognosis of lung cancer patients and its influence on the expression of eIF5 and eIF6.

Methods: The expression of HDAC2, eIF5, and eIF6 in lung cancer tissues was detected by quantitative reverse transcription polymerase chain reaction. The expression correlation between HDAC2 and eIF5 or eIF6 was tested using a t test. The correlation between HDAC2 and eIF5 or eIF6 was analyzed using the TCGA database. The identified cells were constructed with small interfering siRNA and HDAC2 overexpression plasmid. The proliferation and migration ability of the identified cells was investigated by CCK8 and Transwell assays, respectively.

Results: HDAC2, eIF5, and eIF6 were overexpressed in lung cancer tissues, and HDAC2 expression level was negatively correlated with the prognosis of lung cancer patients. HDAC2 expression level was positively correlated with eIF5 and eIF6 expression levels. HDAC2 could regulate the expression of eIF5 and eIF6. The regulation of proliferation and invasion of lung cancer cells by HDAC2 depended on eIF5 and eIF6.

Conclusion: HDAC2, eIF5, and eIF6 were closely related with lung cancer tumorigenesis, which might be potential biological markers and therapeutic targets for lung cancer.
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http://dx.doi.org/10.1007/s11596-021-2389-zDOI Listing
August 2021

Bimetal Cooperatively Catalyzed Arylalkynylation of Alkynylsilanes.

Org Lett 2021 Sep 16;23(17):6724-6728. Epub 2021 Aug 16.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China.

An unprecedented Pd/Rh cooperatively catalyzed arylalkynylation of alkynylsilanes was developed to merge an alkynylidene moiety with benzosilacycle. These silaarenes possess a particular aggregation-induced emission behavior. Mechanistic investigations demonstrate that the relay trimetallic transmetalation plays a pivotal role in governing this transformation.
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http://dx.doi.org/10.1021/acs.orglett.1c02283DOI Listing
September 2021

Potency of miR-144-3p in promoting abdominal aortic aneurysm progression in mice correlates with apoptosis of smooth muscle cells.

Vascul Pharmacol 2021 Aug 5:106901. Epub 2021 Aug 5.

Department of Vascular Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, PR China. Electronic address:

Abdominal aortic aneurysm (AAA), a life-threatening disease, is commonly diagnosed among people with risk factors, including increasing age, male gender, and smoking. The apoptosis of smooth muscle cells (SMCs) has been reported to disrupt the vascular structural integrity, which causes AAA. Thus, we sought to characterize the potential role of microRNA (miR)-144-3p in SMC apoptosis, and to outline the molecular mechanisms involved in this pathway. We collected pathological abdominal aortic tissues and adjacent normal aortic biopsy specimens from 18 patients undergoing AAA repair surgery. The relationship between miR-144-3p expression and SMC proliferation was assessed by transfecting mimic/inhibitor of miR-144-3p in human aortic smooth muscle cells (HASMCs). Anti-growth effect of miR-144-3p and related genes was evaluated in a murine AAA model. Dual luciferase reporter gene assay was adopted to validate the targeting relationship between miR-144-3p and enhancer of zeste homolog 2 (EZH2), and the enrichment of EZH2 in the p21 promoter region was determined by chromatin immunoprecipitation assay. MiR-144-3p was highly expressed in AAA tissues. Enhanced miR-144-3p diminished SMC proliferation by binding to the EZH2 3'-untranslated region and thereby inhibiting EZH2 expression. In addition, EZH2 was highly enriched in the promoter region of p21, and knockdown of p21 expression could rescue the effect of miR-144-3p on SMC proliferation and apoptosis. miR-144-3p serves as a promoter for the apoptosis of SMCs, which contributes to the occurrence and progression of AAA. This observation will serve as the basis for further investigations into potential p21-based therapies for AAA treatment.
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http://dx.doi.org/10.1016/j.vph.2021.106901DOI Listing
August 2021

Achieving enhanced biological phosphorus removal utilizing waste activated sludge as sole carbon source and simultaneous sludge reduction in sequencing batch reactor.

Sci Total Environ 2021 Jul 26;799:149291. Epub 2021 Jul 26.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.

Achieving enhanced biological phosphorus removal dominated by Tetrasphaera utilizing waste activated sludge (WAS) as carbon source could solve the problems of insufficient carbon source and excessive discharge of WAS in biological phosphorus removal. Up to now, the sludge reduction ability of Tetrasphaera remained largely unknown. Furthermore, the difference between traditional sludge fermentation and sludge fermentation dominated by Tetrasphaera was still unclear. In this study, two different sequencing batch reactors (SBRs) were operated. WAS from SBR-parent was utilized as sole carbon source to enrich Tetrasphaera with the relative abundance of 91.9% in SBR-Tetrasphaera. PO-P removal and sludge reduction could simultaneously be achieved. The effluent concentration of PO-P was 0, and the sludge reduction efficiency reached about 44.14% without pretreatment of sludge. Cell integrity detected by flow cytometry, the increase of DNA concentration in the sludge supernatant and decrease of particle size of activated sludge indicated that cell death and lysis occurred in sludge reduction dominated by Tetrasphaera. Stable structure of activated sludge was also damaged in this process, which led to the sludge reduction. By analyzing the excitation-emission matrix spectra of extracellular polymeric substances and the changes of carbohydrate and protein concentration, this study proved that slowly biodegradable organics (e.g., soluble microbial byproduct, tyrosine and tryptophan aromatic protein) could be better hydrolyzed and acidized to volatile fatty acids (VFAs) in sludge fermentation dominated by Tetrasphaera than traditional sludge fermentation, which provided carbon source for biological nutrients removal and saved operation cost in wastewater treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149291DOI Listing
July 2021

Development and multi-level validation of a computational model to predict traumatic aortic injury.

Comput Biol Med 2021 Sep 31;136:104700. Epub 2021 Jul 31.

Center for Applied Biomechanics, University of Virginia, Charlottesville, VA, USA. Electronic address:

Traumatic aortic injury (TAI) is one of the leading causes of fatalities in blunt impact. However, there is no consensus on the injury mechanism of TAI in traffic accidents, mainly due to the complexity of occurrence scenarios and limited real-world crash data relevant to TAI. In this study, a computational model of the aorta with nonlinear mechanical characteristics and accurate morphology was developed and integrated within a thorax finite element model that included all major anatomical structures. To maximize the model's capability for predicting TAI, a multi-level process was presented to validate the model comprehensively. At the component level, the in vitro aortic pressurization testing was simulated to mimic the aortic burst pressure. Then, a sled test of a truncated cadaver was modeled to evaluate aorta response under posterior acceleration. The frontal chest pendulum impact was utilized to validate the performance of the aorta within full body model under direct chest compression. A parametric study was implemented to determine an injury tolerance for the aorta under these different loading conditions. The simulated peak pressure before aortic rupture was within the range of the experimental burst pressure. For the sled test, the simulated chest deflection and cross-sectional pressure of the aorta were correlated with the experimental measurement. No aorta injury was observed in simulated results of both sled test and chest pendulum impact, which matched the experimental findings. The present model will be a useful tool for understanding the TAI mechanisms, evaluating injury tolerance, and developing prevention strategies for aortic injuries.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104700DOI Listing
September 2021

Evaluation and Validation of Thorax Model Responses: A Hierarchical Approach to Achieve High Biofidelity for Thoracic Musculoskeletal System.

Front Bioeng Biotechnol 2021 16;9:712656. Epub 2021 Jul 16.

Center for Applied Biomechanics, University of Virginia, Charlottesville, VA, United States.

As one of the most frequently occurring injuries, thoracic trauma is a significant public health burden occurring in road traffic crashes, sports accidents, and military events. The biomechanics of the human thorax under impact loading can be investigated by computational finite element (FE) models, which are capable of predicting complex thoracic responses and injury outcomes quantitatively. One of the key challenges for developing a biofidelic FE model involves model evaluation and validation. In this work, the biofidelity of a mid-sized male thorax model has been evaluated and enhanced by a multi-level, hierarchical strategy of validation, focusing on injury characteristics, and model improvement of the thoracic musculoskeletal system. At the component level, the biomechanical responses of several major thoracic load-bearing structures were validated against different relevant experimental cases in the literature, including the thoracic intervertebral joints, costovertebral joints, clavicle, sternum, and costal cartilages. As an example, the thoracic spine was improved by accurate representation of the components, material properties, and ligament failure features at tissue level then validated based on the quasi-static response at the segment level, flexion bending response at the functional spinal unit level, and extension angle of the whole thoracic spine. At ribcage and full thorax levels, the thorax model with validated bony components was evaluated by a series of experimental testing cases. The validation responses were rated above 0.76, as assessed by the CORA evaluation system, indicating the model exhibited overall good biofidelity. At both component and full thorax levels, the model showed good computational stability, and reasonable agreement with the experimental data both qualitatively and quantitatively. It is expected that our validated thorax model can predict thorax behavior with high biofidelity to assess injury risk and investigate injury mechanisms of the thoracic musculoskeletal system in various impact scenarios. The relevant validation cases established in this study shall be directly used for future evaluation of other thorax models, and the validation approach and process presented here may provide an insightful framework toward multi-level validating of human body models.
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http://dx.doi.org/10.3389/fbioe.2021.712656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324103PMC
July 2021

Nitrogen removal performance of sulfur autotrophic denitrification under different SO additions using isotopic fractionation of nitrogen and oxygen.

Sci Total Environ 2021 Nov 1;794:148794. Epub 2021 Jul 1.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.

The dual isotope fractionation of nitrogen (N) and oxygen (O) is an effective way to track the transformation of NO-N in biological denitrification process. The Sulfur autotrophic denitrification combined with the different concentrations of SO was investigated using the dual isotope fractionation of nitrogen (N) and oxygen (O) to reveal the nitrogen removal mechanism of the activated sludge. Based on successful autotrophic denitrification incubation, the modified Logistic model responded to the short-term effects of SO addition on NO-N removal and SO generation. Under the SO addition of 0.5, 1, 2 and 4 times of the incubation stage (49.29 mg/L-394.32 mg/L), the fractionation effect of N in NO-N (ε) decreased from 8.74 ± 1.81‰ to 2.08 ± 0.06‰, and the fractionation effect of O in NO-N (ε) declined from 11.34 ± 0.46‰ to 5.48 ± 0.46‰. The ε/ε was maintained at 0.46-0.94, indicating a negative correlation between addition amount and isotope effect, and the addition of high concentrations of SO was not suitable for system stabilization. Moreover, the O-labeled HO (δO) tests significantly proved the presence of O exchange between NO-N/NO-N and HO (67%/97%) during the nitrogen removal process, while the reoxidation of NO-N was explored in the autotrophic denitrification. The kinetic models coupled with isotope fractionation effectively revealed the nitrogen removal characteristics in the autotrophic denitrification systems, and verified the difference between the activated sludge-based wastewater treatment process and the natural ecosystem.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148794DOI Listing
November 2021

FloorLevel-Net: Recognizing Floor-Level Lines With Height-Attention-Guided Multi-Task Learning.

IEEE Trans Image Process 2021 ;30:6686-6699

The ability to recognize the position and order of the floor-level lines that divide adjacent building floors can benefit many applications, for example, urban augmented reality (AR). This work tackles the problem of locating floor-level lines in street-view images, using a supervised deep learning approach. Unfortunately, very little data is available for training such a network - current street-view datasets contain either semantic annotations that lack geometric attributes, or rectified facades without perspective priors. To address this issue, we first compile a new dataset and develop a new data augmentation scheme to synthesize training samples by harassing (i) the rich semantics of existing rectified facades and (ii) perspective priors of buildings in diverse street views. Next, we design FloorLevel-Net, a multi-task learning network that associates explicit features of building facades and implicit floor-level lines, along with a height-attention mechanism to help enforce a vertical ordering of floor-level lines. The generated segmentations are then passed to a second-stage geometry post-processing to exploit self-constrained geometric priors for plausible and consistent reconstruction of floor-level lines. Quantitative and qualitative evaluations conducted on assorted facades in existing datasets and street views from Google demonstrate the effectiveness of our approach. Also, we present context-aware image overlay results and show the potentials of our approach in enriching AR-related applications. Project website: https://wumengyangok.github.io/Project/FloorLevelNet.
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http://dx.doi.org/10.1109/TIP.2021.3096090DOI Listing
January 2021

Circular RNA hsa_circ_0033144 (CircBCL11B) regulates oral squamous cell carcinoma progression via the miR-579/LASP1 axis.

Bioengineered 2021 Dec;12(1):4111-4122

Department of Oral and Maxillofacial surgery, Meizhou People's Hospital, Meizhou, Guangdong Province, China.

Oral squamous cell carcinoma is one of the most common malignant tumors of the head and neck. Increasing evidence suggests that various non-coding RNAs, such as circRNAs, are implicated in a myriad of biological processes supporting tumor progression. Recent studies have revealed that several circRNAs are dysregulated in oral squamous cell carcinoma (OSCC). However, their functional role in OSCC and the underlying mechanism remains to be further investigated. In this study, we aim to evaluate the biological role and survey the molecular mechanism of circBCL11B in regulating the progression of OSCC. We demonstrated that circBCL11B was significantly upregulated in OSCC tissues and cell lines, and the expression level was correlated with the malignancy. Silencing cirCBCL11B inhibited cell proliferation and migration, and also included cell apoptosis in OSCC cells. miR-145 was identified as a downstream target mediating the effect of circBCL11B by targeting LASP1. miR-145 negatively regulated LASP1 expression, which could be rescued by miR-145 inhibitor. Collectively, our study uncovered a functional role of circBCL11B/miR-579/LASP1 axis in OSCC, implying that targeting these molecules could be an intervention approach in OSCC treatment.
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http://dx.doi.org/10.1080/21655979.2021.1953214DOI Listing
December 2021

The Impact of General Self-Efficacy on Psychological Resilience During the COVID-19 Pandemic: The Mediating Role of Posttraumatic Growth and the Moderating Role of Deliberate Rumination.

Front Psychol 2021 23;12:684354. Epub 2021 Jun 23.

Office of International Cooperation and Exchange, Dongguan University of Technology, Dongguan, China.

This study used a moderated mediation model to explore the relationship between general self-efficacy (GSE) and psychological resilience (PR) and the associated mechanisms, the mediating role of posttraumatic growth (PTG), and the moderating role of deliberate rumination (DR) during the coronavirus disease 2019 (COVID-19) pandemic. Knowledge of the relationship between these four variables examined further understanding of the PR improvement mechanism of college students and even the general public. The college students who participated in this study came from an independent college in Guangdong Province, China. A total of 918 college students completed the survey, and the final data sample size was 881. SPSS 23.0 and PROCESS (version 3.3) were used to conduct Pearson's correlation analysis and hierarchical regression linear analysis on the data. (1) The correlation analysis showed that GSE and PR were positively correlated and that PTG was positively correlated with GSE and PR. DR was positively correlated with GSE, PTG, and PR. (2) The results of mediation analysis showed that GSE had a direct predictive effect on DR, and PTG partially mediated the relationship between the two. (3) The results of moderating effect analysis showed that DR hindered the effect of GSE on PTG but enhanced the positive impact of PTG on PR. General self-efficacy can improve PR under the mediating influence of PTG. DR played a positive moderating role in the relationship between GSE and PTG, and played a negative moderating role in the relationship between PTG and PR. These results advance the understanding of the mechanism between GSE and PR.
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http://dx.doi.org/10.3389/fpsyg.2021.684354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261126PMC
June 2021

GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis-elements.

New Phytol 2021 Jul 10. Epub 2021 Jul 10.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.

Cotton fibre is the most important source for natural textiles. The secondary cell walls (SCWs) of mature cotton fibres contain the highest proportion of cellulose content (> 90%) in any plant. The onset and progression of SCW cellulose synthesis need to be tightly controlled to balance fibre elongation and cell wall deposition. However, regulatory mechanisms that control cellulose synthesis during cotton fibre growth remain elusive. Here, we conducted genetic and functional analyses demonstrating that the R2R3-MYB GhMYB7 controls cotton fibre cellulose synthesis. Overexpression of GhMYB7 in cotton sped up SCW cellulose biosynthesis in fibre cells, and led to shorter fibres with thicker walls. By contrast, RNA interference (RNAi) silencing of GhMYB7 delayed fibre SCW cellulose synthesis and resulted in elongated fibres with thinner walls. Furthermore, we demonstrated that GhMYB7 regulated cotton fibre SCW cellulose synthases by directly binding to three distinct cis-elements in the respective GhCesA4, GhCesA7 and GhCesA8 promoters. We found that this regulatory mechanism of cellulose synthesis was 'hi-jacked' also by other GhMYBs. Together, our findings uncover a hitherto-unknown mechanism that cotton fibre employs to regulate SCW cellulose synthesis. Our results also provide a strategy for genetic improvement of SCW thickness of cotton fibre.
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http://dx.doi.org/10.1111/nph.17612DOI Listing
July 2021

Involvement of glucosinolates in the resistance to zinc oxide nanoparticle-induced toxicity and growth inhibition in .

Environ Sci Process Impacts 2021 Jul;23(7):1040-1049

Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, Zhejiang, China.

Zinc oxide nanoparticles (ZnO NPs) are widely used to manufacture textile fibers, synthetic rubber, and paint. However, crop yields and quality are threatened by the increased use of metallic NPs in industry, which has resulted in their accumulation in agricultural land. Many studies have shown that plants defend against biotic and abiotic stresses through the activities of metabolites and hormones. However, whether glucosinolates (GSs) are involved in plant responses to ZnO NP-related stress remains unknown. In this study, wild-type (WT) and GS mutant (myb28/29 and cyp79B2/B3) Arabidopsis plants were subjected to ZnO NP stress to address this question. Our results showed that exposure to ZnO NPs promoted GS accumulation and induced the relative messenger RNA (mRNA) expression levels of GS biosynthesis-related genes. Moreover, ZnO NP treatment adversely affected root length, the number of lateral roots, chlorophyll contents, and plant biomass. Importantly, our results showed that root growth, chlorophyll contents, and plant biomass were all decreased in the GS mutants compared with those in WT plants. Overall, our results showed that WT plants tolerated ZnO NP-induced stress more efficiently than the GS mutants, suggesting that GSs are involved in plant resistance to ZnO NP-induced toxicity.
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http://dx.doi.org/10.1039/d1em00134eDOI Listing
July 2021

Performances and mechanisms of simultaneous nitrate and phosphate removal in sponge iron biofilter.

Bioresour Technol 2021 Oct 9;337:125390. Epub 2021 Jun 9.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.

Sponge iron is a potential material for nitrogen and phosphate removal. To explore the performances and mechanisms of nitrogen and phosphate removal by sponge iron, a sponge iron biofilter was established. The results showed that nitrate was completely removed at HRT of 48 h without external carbon source and at HRT of 3 h with C/N ratio of 5. Furthermore, it was easy to achieve partial denitrification at HRT of 1 h with C/N ratio of 3. The mechanisms of nitrate removal were chemical reduction and nitrate dependent ferrous oxidation without external carbon source and heterotrophic denitrification with external carbon source. XPS result indicated that phosphate was removed by the formation of ferric phosphate precipitation. High throughput sequencing showed that iron-oxidizing bacteria Gallionellaceae was highly enriched in biofilter, accounting for 17.83%, which indicated that it was feasible to achieve autotrophic nitrate removal by sponge iron biofilter.
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http://dx.doi.org/10.1016/j.biortech.2021.125390DOI Listing
October 2021

Phosphorus recovery from waste activated sludge by sponge iron seeded crystallization of vivianite and process optimization with response surface methodology.

Environ Sci Pollut Res Int 2021 Jun 11. Epub 2021 Jun 11.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental and Biological Sciences, Beijing University of Technology, Pingleyuan No.100, Chaoyang District, Beijing, 100124, China.

As a novel phosphorus recovery product, vivianite (Fe(PO)·8HO) has attracted much attention due to its enormous recycling potential and foreseeable economic value. Taking sponge iron as seed material, the effect of different reaction conditions on the recovery of phosphorus in waste activated sludge by vivianite crystallization was studied. Through single factor tests, the optimal conditions for vivianite formation were in the pH range of 5.5-6.0 with Fe/P molar ratio of 1.5. Scanning electron microscopy (SEM), powder X-ray diffraction (XRD), and energy dispersive spectroscopy (EDS) were used to analyze the components of the crystals. The results showed that the vivianite produced by sponge iron as the seed crystal were larger and thicker (300-700 μm) than other seed (200-300 μm) and without seed (50-100 μm). Moreover, vivianite, which was synthesized with sponge iron as seed, was obviously magnetic and could be separated from the sludge by rubidium magnet. The Box-Behnken design of the response surface methodology was used to optimize the phosphorus-recovery process with sponge iron (maximum phosphorus recovery rate was 83.17%), and the interaction effect of parameters was also examined, pH had a significant effect on the formation of vivianite. In summary, this research verifies the feasibility of using sponge iron as the seed crystal to recover phosphorus in the form of vivianite from waste activated sludge, which is conducive to the subsequent separation and utilization of vivianite.
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http://dx.doi.org/10.1007/s11356-021-14561-7DOI Listing
June 2021

Biochemical and Functional Characterization of GALT8, an GT31 β-(1,3)-Galactosyltransferase That Influences Seedling Development.

Front Plant Sci 2021 25;12:678564. Epub 2021 May 25.

ARC Centre of Excellence on Plant Cell Walls, School of BioSciences, The University of Melbourne, Melbourne, VIC, Australia.

Arabinogalactan-proteins (AGPs) are members of the hydroxyproline-rich glycoprotein (HRGP) superfamily, a group of highly diverse proteoglycans that are present in the cell wall, plasma membrane as well as secretions of almost all plants, with important roles in many developmental processes. The role of GALT8 (At1g22015), a Glycosyltransferase-31 (GT31) family member of the Carbohydrate-Active Enzyme database (CAZy), was examined by biochemical characterization and phenotypic analysis of a mutant line. To characterize its catalytic function, GALT8 was heterologously expressed in tobacco leaves and its enzymatic activity tested. GALT8 was shown to be a β-(1,3)-galactosyltransferase (GalT) that catalyzes the synthesis of a β-(1,3)-galactan, similar to the activity of KNS4/UPEX1 (At1g33430), a homologous GT31 member previously shown to have this activity. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed the products were of 2-6 degree of polymerisation (DP). Previous reporter studies showed that is expressed in the central and synergid cells, from whence the micropylar endosperm originates after the fertilization of the central cell of the ovule. Homozygous mutants have multiple seedling phenotypes including significantly shorter hypocotyls and smaller leaf area compared to wild type (WT) that are attributable to defects in female gametophyte and/or endosperm development. was shown to partially complement the mutant phenotypes in genetic complementation assays suggesting a similar but not identical role compared to in β-(1,3)-galactan biosynthesis. Taken together, these data add further evidence of the important roles GT31 β-(1,3)-GalTs play in elaborating type II AGs that decorate AGPs and pectins, thereby imparting functional consequences on plant growth and development.
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http://dx.doi.org/10.3389/fpls.2021.678564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186459PMC
May 2021

Constitutive expression of transcription factor SirZ blocks pathogenicity in Leptosphaeria maculans independently of sirodesmin production.

PLoS One 2021 10;16(6):e0252333. Epub 2021 Jun 10.

School of BioSciences, The University of Melbourne, Melbourne, Victoria, Australia.

Sirodesmin, the major secondary metabolite produced by the plant pathogenic fungus Leptosphaeria maculans in vitro, has been linked to disease on Brassica species since the 1970s, and yet its role has remained ambiguous. Re-examination of gene expression data revealed that all previously described genes and two newly identified genes within the sir gene cluster in the genome are down-regulated during the crucial early establishment stages of blackleg disease on Brassica napus. To test if this is a strategy employed by the fungus to avoid damage to and then detection by the host plant during the L. maculans asymptomatic biotrophic phase, sirodesmin was produced constitutively by overexpressing the sirZ gene encoding the transcription factor that coordinates the regulation of the other genes in the sir cluster. The sirZ over-expression strains had a major reduction in pathogenicity. Mutation of the over-expression construct restored pathogenicity. However, mutation of two genes, sirP and sirG, required for specific steps in the sirodesmin biosynthesis pathway, in the sirZ over-expression background resulted in strains that were unable to synthesize sirodesmin, yet were still non-pathogenic. Elucidating the basis for this pathogenicity defect or finding ways to overexpress sirZ during disease may provide new strategies for the control of blackleg disease.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252333PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191991PMC
June 2021

Exploring collective identity of a group of teaching-oriented academics amid research discourse: a Chinese case.

High Educ (Dordr) 2021 Jun 4:1-18. Epub 2021 Jun 4.

College of Education, University of Canterbury, Christchurch, New Zealand.

This article explores how a group of teaching-oriented academics-College English (CE) teachers in China-negotiate their collective identity at the workplace amid research discourse. Drawing on an integrated theoretical framework focusing on the interrelationship between discourse, practice, and social networks, the study reveals the field of research where CE teachers lived imbued by multiple, dynamic discourses and power relations, i.e., the discourse of illegitimate pedagogic research v. the discourse of officially valued research, the discourse of the lower-status department oriented to teaching and public service v. the higher-status, disciplinary department. Drawing upon discursive resources, CE teachers positioned themselves as marginal pedagogic researchers and inferior CE teachers. Meanwhile, CE teachers constructed their identity-in-practice as pedagogic researchers by developing a pedagogic research community through their daily teaching practice. The contradiction between identity-in-discourse and identity-in-practice suggests the complexity of teaching-oriented, public service-oriented academics' professional life and the predicament they face, such as the limited access to research networks, and the lack of shared understanding in constructing a pedagogic research community. The study argues for more attention to be given to these academics who occupy the bottom of academic hierarchy in higher education context and might be the most vulnerable group in the midst of the COVID-19 pandemic.
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http://dx.doi.org/10.1007/s10734-021-00728-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175435PMC
June 2021

Supercapacitive brophene-graphene aerogel as elastic-electrochemical dielectric layer for sensitive pressure sensors.

J Colloid Interface Sci 2021 Nov 25;601:355-364. Epub 2021 May 25.

Science and Technology Institute, Hubei Key Laboratory of Advanced Textile Materials & Application, Laboratory for Electron Microscopy, Wuhan Textile University, Wuhan 430073, Hubei, People's Republic of China.

A sensitive pressure sensor based on ultralight and superelastic supercapacitive borophene-graphene aerogel as dielectric layer is reported. The borophene-graphene aerogel not only combines large specific surface area of reduced graphene oxide and high conductivity of borophene, but also exhibits rich porous structure. The strong synergy and intercalation between two different two-dimensional materials benefit electron transfer and electrolyte ion diffusion. On the one hand, the aerogel exhibits greater mass specific capacitance of 330 F g than pure graphene aerogel. More importantly, serving as dielectric layer for pressure sensors with a symmetrical structure, the sensor represents ultra-high sensitivity (0.90 KPa) in the pressure range (<3 KPa), ultra-rapid response time (~110 ms), ultra-low detection limit as 8.7 Pa and excellent working stability after 1000 cycles. In practical application, the sensor demonstrates great performance in monitoring human physiological signals, and agricultural applications.
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http://dx.doi.org/10.1016/j.jcis.2021.05.116DOI Listing
November 2021

Photocatalyzed cycloaromatization of vinylsilanes with arylsulfonylazides.

Nat Commun 2021 06 3;12(1):3304. Epub 2021 Jun 3.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.

Sila-molecules have recently attracted attention due to their promising applications in medical and industrial fields. Compared with all-carbon parent compounds, the different covalent radius and electronegativity of silicon from carbon generally endow the corresponding sila-analogs with unique biological activity and physicochemical properties. Vinylsilanes feature both silyl-hyperconjugation effect and versatile reactivities, developing vinylsilane-based Smiles rearrangement will therefore provide an efficient platform to assemble complex silacycles. Here we report a practical Ir(III)-catalyzed cycloaromatization of ortho-alkynylaryl vinylsilanes with arylsulfonyl azides for delivering naphthyl-fused benzosiloles under visible-light photoredox conditions. The combination of experiments and density functional theory (DFT) energy profiles reveals the reaction mechanism involving α-silyl radical Smiles rearrangement.
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http://dx.doi.org/10.1038/s41467-021-23326-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175346PMC
June 2021

[Wastewater Treatment Effects of Ferric-carbon Micro-electrolysis and Zeolite in Constructed Wetlands].

Huan Jing Ke Xue 2021 Jun;42(6):2875-2884

State Cultivation Base of Eco-agriculture for Southwest Mountainous Land, College of Resources and Environment, Southwest University, Chongqing 400716, China.

Ferric-carbon micro-electrolysis fillers and zeolite have been increasingly used as substrates in constructed wetlands due to their good wastewater pollution-removal efficiencies. To explore the effects of different fillers on wastewater treatment in constructed wetlands, four constructed wetlands were examined with vertical subsurface flow areas filled with ferric-carbon micro-electrolysis filler+gravel (CW-A), ferric-carbon micro-electrolysis filler+zeolite (CW-B), zeolite (CW-C), and gravel (CW-D). In addition, intermittent aeration was used to improve the dissolved oxygen (DO) environment. The results showed that, compared with CW-D, the ferric-carbon micro-electrolysis filler significantly increased the dissolved oxygen (DO, <0.05) and pH (<0.05) of the effluent from the wetlands. The mean removal efficiency of chemical oxygen demand (COD) in the four constructed wetlands were more than 95% (>0.05). For TN, the mean removal efficiency of CW-A,-B, and-C was 7.94% (<0.05), 9.29% (<0.05), and 3.63% (<0.05) higher than that of CW-D, respectively. The contribution of ferric-carbon micro-electrolysis filler and zeolite to improving the TN removal efficiency of the constructed wetlands was 73.55% and 26.45%, respectively. The mean removal efficiency of NH in the four wetlands ranged from 67.93% to 76.90%, and compared with CW-D, the other treatments significantly improved the removal efficiency of NH (<0.05). The ferric-carbon micro-electrolysis filler had an excellent removal effect on NO, with a removal efficiency of more than 99%, which was significantly higher than the constructed wetlands without ferric-carbon micro-electrolysis (<0.05). Considering the treatment effect of the organic pollutants and the nitrogen-containing pollutants, CW-B achieved the best removal efficiency in constructed wetlands with intermittent aeration.
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http://dx.doi.org/10.13227/j.hjkx.202010200DOI Listing
June 2021

Depression and Creativity During COVID-19: Psychological Resilience as a Mediator and Deliberate Rumination as a Moderator.

Front Psychol 2021 6;12:665961. Epub 2021 May 6.

School of Geography, South China Normal University, Guangzhou, China.

The pandemic of coronavirus disease 2019 (COVID-19), which has had a significant impact on people's lives, has apparently increased the incidence of depression. Although the topic of how depression affects creativity is contested, previous research has revealed a significant relationship between the two. The purpose of this study is to further investigate the relationship and the mechanisms that operate between depression and creativity. A total of 881 students at an independent college in China completed a questionnaire consisting of the Self-Reported Depression Scale, Runco Ideational Behavior Scale, Psychological Resilience Scale, Deliberate Rumination Scale and demographic information. Among the respondents, 317 (36.0%) were male and 564 (64.0%) were female, all of whom were from the same grade. Correlation analyses were conducted, and then the researchers carried out mediation analysis and developed a moderated mediation model. The results indicated that (a) depression was positively related to creativity ( = 0.085, < 0.05); (b) psychological resilience mediated the relationship between depression and creativity; specifically, psychological resilience was negatively related to depression ( = -0.462, < 0.01), which in turn was positively related to creativity ( = 0.198, < 0.01); and (c) deliberate rumination moderated the relationship between depression and psychological resilience, showing a significant negative correlation with depression ( = 0.138, < 0.01), psychological resilience ( = 0.078, < 0.05), and creativity ( = 0.288, < 0.05); specifically, higher levels of deliberate rumination strengthened the negative correlation between psychological resilience and depression. The results suggest that depression is a positive predictor of creativity and may promote creativity to some extent. Further, individuals with greater psychological resilience are more creative than those with less psychological resilience, as it is a question of whether they can and to what extent they can effectively use depression as an emotional resource. Last, an individual's level of deliberate rumination moderates the mediating process, especially at the stage where depression is associated with psychological resilience. These findings advance understanding of the mechanisms that operate between depression and creativity.
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http://dx.doi.org/10.3389/fpsyg.2021.665961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134673PMC
May 2021

The flavor of Chinese kale sprouts is affected by genotypic variation of glucosinolates and their breakdown products.

Food Chem 2021 Oct 20;359:129824. Epub 2021 Apr 20.

Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China. Electronic address:

Metabolic profiling of glucosinolates and their breakdown products in sprouts of 22 Chinese kale (Brassica oleracea var. alboglabra, BOA) varieties were investigated by using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). Relationships between glucosinolate metabolites and flavor of Chinese kale sprouts were also analyzed. Results showed that compositions and contents of both glucosinolates and their breakdown products varied greatly among different varieties of Chinese kale sprouts. Gluconapin and 4,5-Epithio-pentanenitrile were the dominant glucosinolate and glucosinolate breakdown product in Chinese kale sprouts, respectively. Gluconapin and glucobrassicin were significantly related to bitterness (r = 0.577, 0.648, respectively; p < 0.05). BOA 1 and BOA 13, BOA 3 and BOA 10 are good candidates for future breeding programs since the former two varieties have light bitterness and pungency, and the latter two varieties contain high levels of glucosinolate breakdown products such as isothiocyanates and epithionitriles in sprouts.
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http://dx.doi.org/10.1016/j.foodchem.2021.129824DOI Listing
October 2021

Rh(III)-Catalyzed Csp-Csp σ-Bond Enolation of α-Indolyl Alcohols.

Org Lett 2021 May 6;23(10):3965-3969. Epub 2021 May 6.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China.

A Rh(III)-catalyzed Csp-Csp σ-bond carbenoid functionalization of α-(2-indolyl)alcohols with acceptor/acceptor diazo compounds has been developed. This transformation provides an efficient strategy to assemble stable C2-enolated indole skeletons via Csp-Csp σ-bond cleavage.
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http://dx.doi.org/10.1021/acs.orglett.1c01132DOI Listing
May 2021
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