Publications by authors named "Wei Yao"

632 Publications

Anion binding to ubiquitin and its relevance to the Hofmeister effects.

Chem Sci 2020 Nov 4;12(1):320-330. Epub 2020 Nov 4.

Department of Chemistry, Tulane University New Orleans LA 70118 USA

Although the non-covalent interactions between proteins and salts contributing to the Hofmeister effects have been generally mapped, there are many questions regarding the specifics of these interactions. We report here studies involving the small protein ubiquitin and salts of polarizable anions. These studies reveal a complex interplay between the reverse Hofmeister effect at low pH, the salting-in Hofmeister effect at higher pH, and six anion binding sites in ubiquitin at the root of these phenomena. These sites are all located at protuberances of preorganized secondary structure, and although stronger at low pH, are still apparent when ubiquitin possesses no net charge. These results demonstrate the traceability of these Hofmeister phenomena and suggest new strategies for understanding the supramolecular properties of proteins.
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http://dx.doi.org/10.1039/d0sc04245eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178748PMC
November 2020

Naringin attenuates rat myocardial ischemia/reperfusion injury via PI3K/Akt pathway-mediated inhibition of apoptosis, oxidative stress and autophagy.

Exp Ther Med 2021 Aug 28;22(2):811. Epub 2021 May 28.

Department of Cardiology, Suizhou Hospital, Hubei University of Medicine, Suizhou, Hubei 441300, P.R. China.

Naringin (NRG) has been reported to exert cardioprotective effects against multiple cardiovascular diseases, including lipopolysaccharide-induced and hyperglycemia-induced myocardial injury. However, the role of NRG in myocardial ischemia/reperfusion (I/R) injury remains unclear. In the present study, the PI3K/Akt pathway was investigated to evaluate the possible mechanisms underlying the roles of NRG in myocardial ischemia/reperfusion (I/R) injury. The levels of cardiac enzymes were measured by ELISA to evaluate the optimal dosage of NRG that could protect against myocardial I/R injury. Rats were administered 100 mg/kg of NRG and activities of myocardial enzymes, the level of cardiac apoptosis and inflammation, oxidant response, autophagy indicators and echocardiography were evaluated. The level of corresponding proteins was measured using western blotting. The results indicated that NRG elicited the best cardioprotective effects at a dose of 100 mg/kg by significantly reducing the levels of myocardial enzymes, apoptosis, inflammation, oxidative response and infarct size. Furthermore, NRG alleviated contractile dysfunction by increasing the left ventricular ejection fraction and fractional shortening. In addition, NRG markedly promoted the phosphorylation of Akt, while decreasing the level of autophagy indicator beclin-1 and the microtubule-associated protein 1B-light chain 3 (LC3B) II/ LC3BI ratio. However, PI3K/Akt inhibitor (LY294002) partially reduced the NRG induced phosphorylation of Akt and the reduction in beclin-1, along with the LC3BII/LC3BI ratio. The results of the present study demonstrated that NRG could attenuate myocardial I/R injury.
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http://dx.doi.org/10.3892/etm.2021.10243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193209PMC
August 2021

Experimental Evidence of Chiral Symmetry Breaking in Kekulé-Ordered Graphene.

Phys Rev Lett 2021 May;126(20):206804

State Key Laboratory of Low-Dimensional Quantum Physics and Department of Physics, Tsinghua University, Beijing 100084, People's Republic of China.

The low-energy excitations of graphene are relativistic massless Dirac fermions with opposite chiralities at valleys K and K^{'}. Breaking the chiral symmetry could lead to gap opening in analogy to dynamical mass generation in particle physics. Here we report direct experimental evidences of chiral symmetry breaking (CSB) from both microscopic and spectroscopic measurements in a Li-intercalated graphene. The CSB is evidenced by gap opening at the Dirac point, Kekulé-O type modulation, and chirality mixing near the gap edge. Our work opens up opportunities for investigating CSB related physics in a Kekulé-ordered graphene.
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http://dx.doi.org/10.1103/PhysRevLett.126.206804DOI Listing
May 2021

Selective PDE1 inhibition ameliorates vascular function, reduces inflammatory response, and lowers blood pressure in ageing animals.

J Pharmacol Exp Ther 2021 Jun 7. Epub 2021 Jun 7.

Erasmus MC, Netherlands

Diminished nitric oxide - cGMP -mediated relaxation plays a crucial role in cardiovascular ageing, leading to decreased vasodilation, vascular hypertrophy and stiffening, and ultimately cardiovascular dysfunction. Ageing is the time-related worsening of physiological function due to complex cellular and molecular interactions, and is at least partly driven by DNA damage. Genetic deletion of the DNA repair enzyme ERCC1 endonuclease in mice provides us an efficient tool to accelerate vascular ageing, explore mechanisms, and test potential treatments. Previously we identified the cGMP-degrading enzyme phosphodiesterase 1 as a potential treatment target in vascular ageing. In the present study, we studied the effect of acute and chronic treatment with ITI-214, a selective phosphodiesterase 1 inhibitor on vascular ageing features in mice. Compared to wild-type mice, mice at the age of 14 weeks showed decreased reactive hyperemia, diminished endothelium-dependent and -independent responses of arteries in organ baths, carotid wall hypertrophy, and elevated circulating levels of inflammatory cytokines. Acute ITI-214 treatment in organ baths restored the arterial endothelium-independent vasodilation in mice. An 8-week treatment with 100 mg/kg/d ITI-214 improved endothelium-independent relaxation in both aorta and coronary arteries, at least partly restored the diminished reactive hyperemia, lowered the systolic and diastolic blood pressure, normalized the carotid hypertrophy, and ameliorated inflammatory responses exclusively in mice. These findings suggest PDE1 inhibition would provide a powerful tool for nitric oxide - cGMP augmentation and have significant therapeutic potential to battle arteriopathy related to ageing. The findings implicate the key role of PDE1 in vascular function and might be of clinical importance for prevention of mortalities and morbidities related to vascular complications during ageing, as well as for progeria patients that show a high risk of cardiovascular disease.
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http://dx.doi.org/10.1124/jpet.121.000628DOI Listing
June 2021

Attention Enhancement for Exoskeleton-Assisted Hand Rehabilitation Using Fingertip Haptic Stimulation.

Front Robot AI 2021 21;8:602091. Epub 2021 May 21.

Department of Mechanical Engineering, University College London, London, United Kingdom.

Active enrollment in rehabilitation training yields better treatment outcomes. This paper introduces an exoskeleton-assisted hand rehabilitation system. It is the first attempt to combine fingertip cutaneous haptic stimulation with exoskeleton-assisted hand rehabilitation for training participation enhancement. For the first time, soft material 3D printing techniques are adopted to make soft pneumatic fingertip haptic feedback actuators to achieve cheaper and faster iterations of prototype designs with consistent quality. The fingertip haptic stimulation is synchronized with the motion of our hand exoskeleton. The contact force of the fingertips resulted from a virtual interaction with a glass of water was based on data collected from normal hand motions to grasp a glass of water. System characterization experiments were conducted and exoskeleton-assisted hand motion with and without the fingertip cutaneous haptic stimulation were compared in an experiment involving healthy human subjects. Users' attention levels were monitored in the motion control process using a Brainlink EEG-recording device and software. The results of characterization experiments show that our created haptic actuators are lightweight (6.8 ± 0.23 g each with a PLA fixture and Velcro) and their performance is consistent and stable with small hysteresis. The user study experimental results show that participants had significantly higher attention levels with additional haptic stimulations compared to when only the exoskeleton was deployed; heavier stimulated grasping weight (a 300 g glass) was associated with significantly higher attention levels of the participants compared to when lighter stimulated grasping weight (a 150 g glass) was applied. We conclude that haptic stimulations increase the involvement level of human subjects during exoskeleton-assisted hand exercises. Potentially, the proposed exoskeleton-assisted hand rehabilitation with fingertip stimulation may better attract user's attention during treatment.
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http://dx.doi.org/10.3389/frobt.2021.602091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176106PMC
May 2021

circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: Selected Key circRNAs in Neuroblastoma and Their Associations with Clinical Features.

Cancer Manag Res 2021 28;13:4271-4281. Epub 2021 May 28.

Department of Pediatric Surgery, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, People's Republic of China.

Objective: The roles of circRNAs in neuroblastoma (NB) are unclear. We used next-generation sequencing to detect the circRNA expression profiles in NB to identify the key circRNAs and analyzed the relationships between the circRNAs and clinical features.

Methods: Five paired neuroblastoma tumor and adjacent normal fetal adrenal medulla samples were collected for high-throughput RNA sequencing. Bioinformatics analysis was performed for functional annotation of the host genes of differentially expressed circRNAs. Validation of dysregulated circRNAs was performed by real-time quantitative reverse transcription polymerase chain reaction. The relationships between the key circRNAs and clinical features were analyzed. In addition, overexpression of key circRNAs in an NB cell line, as well as cell proliferation assays, colony formation assays and cell migration assays, was conducted to investigate the biological functions of key circRNAs.

Results: A total of 4704 differentially expressed circRNAs were found, including 2462 up-regulated and 2242 down-regulated circRNAs. According to our previous studies, the predicted target circRNAs of miR-21 involved in tumorigenic signaling pathways were selected, including circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3. These circRNAs were associated with clinical features, and the circRNA expression was significantly lower (P < 0.05) in the NB tissues than in normal adrenal tissues. Overexpression of circRNA-TBC1D4 promotes NB cell migration, but not proliferation and colony-formation in vitro.

Conclusion: We suggest that circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3 may be cancer suppressor genes, which act by sponging miR-21 in NB. Further investigations are needed to elucidate the underlying mechanism.
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http://dx.doi.org/10.2147/CMAR.S297316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168971PMC
May 2021

MORPHOLOGY OF ADULTS AND IMMATURES OF THE ACANTHOCEPHALAN, POMPHORHYNCHUS FUHAIENSIS YUE, 1998 (ACANTHOCEPHALA: POMPHORHYNCHIDAE) FROM CYPRINID FISH IN NORTHWEST CHINA.

J Parasitol 2021 May;107(3):446-454

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 7 South Donghu Road, Wuchang District, Wuhan, Hubei Province, 430072, China.

Adult specimens of Pomphorhynchus fuhaiensis were identified from common carp (Cyprinus carpio L.) in Ulungur Lake of northwest China, and acanthors, acanthellae, cystacanths dissected from Gammarus lacustris in a small tributary of Ulungur River for the first time. The acanthocephalans were also found in crucian carp (Carassius carassius L.), tench (Tinca tinca L.), oriental bream (Abramis brama orientalis Berg), and ide (Leuciscus idus L.) in the lake. This species is distinguished from other species in Pomphorhynchus by its large, spherical bulb and very long neck as well as by a cylindrical proboscis armed with 15-17 longitudinal rows of 9-12 hooks each. The anterior proboscis hooks are almost uniform in size and shape, the sixth hook in a short row and the seventh hook in long row decrease abruptly in size posteriorly with the last end hook being a little larger than the prebasal hook, and in a ring; posterior proboscis hooks much more widely spaced. Furthermore, the lemnisci are claviform. The mean neck:trunk ratio is about 0.5, which is larger than most other species in Pomphorhynchus. Females are larger than males. In males, the testes are in one-third to one-half of the trunk, equal, ovoid-spheroid, usually contiguous, and small relative to the body size, and there are 6 ovoid cement glands. Pomphorhynchus fuhaiensis is similar to Pomphorhynchus laevis but can be distinguished by the number of longitudinal rows of hooks. Pomphorhynchus laevis is armed with 18-20 longitudinal rows of 11-13 hooks, P. fuhaiensis is armed with 15-17 longitudinal rows of 9-12 hooks.
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http://dx.doi.org/10.1645/13-228DOI Listing
May 2021

The Application of and Factors Influencing, the NB5 Assay in Neuroblastomas.

Front Oncol 2021 14;11:633106. Epub 2021 May 14.

Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.

Purpose: The NB5 assay was performed in bone marrow (BM) and peripheral blood (PB) to detect neuroblastomas (NBs) with micrometastases. The sensitivity and factors influencing the NB5 assay were preliminarily evaluated.

Methods: The NB5 assay uses RT-PCR to detect the co-expression of five mRNAs from the neuroblastoma-associated genes, CHGA, DCX, DDC, PHOX2B, and TH. We enrolled 180 cases of neuroblastoma and 65 cases of non-neuroblastoma. Bone marrow and peripheral blood were collected from every patient. The gold standard for the diagnosis of NB was pathological evaluation of solid tumor specimens or bone marrow biopsies (BMBs) from hematological tumors. STATA version 15 and SPSS version 17 software were used for analysis.

Results: We found that 17 patients were BMB (+), and they were diagnosed as the International Neuroblastoma Staging System (INSS) stage IV and the high-risk group. All 17 patients were BM (+), while 15 patients were PB (+) (15/17, 88.2%). Among the 163 children who were BMB (-), 56 were BM (+), 40 were PB (+), and 36 were BM (+) and PB (+). The sensitivity of the NB5 assay in BM (40.5%) and PB (30.5%) was significantly higher than the sensitivity of BMB (9.4%, P = 0.000). In the non-NB group, four cases were BM (+) and one case was PB (+). The specificity of the NB5 assay in BM and PB was 93.8% and 98.5%, respectively. The sensitivity of the NB5 assay in both BM and PB in INSS stage IV patients was significantly higher than that in INSS stage I-II patients (P <0.05). The sensitivity of the NB5 assay in both BM and PB in the high-risk group was significantly higher than that in the middle-low-risk groups (P = 0.0001). Logistic regression analyses indicated that liver metastases and bone metastases were the primary factors influencing the sensitivity of the NB5 assay in BM and PB (P <0.05).

Conclusions: The NB5 assay had significantly higher sensitivity than the pathological analysis of BMB in detecting NB with micrometastases. The NB5 assay had higher sensitivity in INSS stage IV or the high-risk group. Liver metastases and bone metastases were the primary factors that affected the sensitivity of the NB5 assay.
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http://dx.doi.org/10.3389/fonc.2021.633106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162211PMC
May 2021

Psychometric Properties of the Chinese Version of the Sarcopenia and Quality of Life, a Quality of Life Questionnaire Specific for Sarcopenia.

Calcif Tissue Int 2021 May 15. Epub 2021 May 15.

Chemistry, National University of Singapore, No. 21, Lower Kent Ridge Road, Singapore, 119077, Singapore.

A quality of life questionnaire specific to sarcopenia (SarQoL®) was successfully developed. There is a huge demand for translation and validation in Chinese. The aim of this study was to translate the SarQoL® into Chinese and investigate its psychometric properties. The translation and cross-cultural adaptation process recommended by the developers of the initial questionnaire was followed. A total of 159 participants were investigated. The translation process consists of five steps: (1) two bilinguals independently translate initial English to Chinese; (2) synthesize the two translations into one; (3) backward translations; (4) expert committee review and (5) test of the pre-final version. The validation consists of three parts: (1) validity (discriminative power, construct validity); (2) reliability (internal consistency, test-retest reliability) and (3) floor and ceiling effects. There was no difficulty in translation process. Regarding the validity, good discriminant validity {quality of life for sarcopenic subjects [35.56 (29.73-42.70)] vs. non-sarcopenic ones [73.22 (60.09-82.90)], p < 0.001} and consistent construct validity [high correlations (spearman's r) of SarQoL® with generic Short Form-36 version 2 questionnaire (0.250 to 0.824) and EuroQoL-5-Dimension questionnaire (- 0.114 to - 0.823)] were found in SarQoL®. Regarding reliability, high internal consistency (Cronbach's alpha coefficient was 0.867) and excellent test-retest reliability (intraclass coefficient correlation was 0.997, 95% CI 0.994-0.998) were found. No ceiling/floor effect was reflected. A valid SarQoL® questionnaire is now available for Chinese population. It can provide a better understanding of the sarcopenia disease burden and serve as a therapeutic outcome indicator in research.
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http://dx.doi.org/10.1007/s00223-021-00859-8DOI Listing
May 2021

CircRNA expression profiling and bioinformatics analysis indicate the potential biological role and clinical significance of circRNA in influenza A virus-induced lung injury.

J Biosci 2021 ;46

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215000, China.

Circular RNA (circRNA) plays an important role in the regulation of multiple biological processes. However, circRNA profiling and the potential biological role of circRNA in influenza A virus (IAV)-induced lung injury have not been investigated. In the present study, circRNA expression profiles in lung tissues from mice with and without IAV-induced lung injury were analyzed using high-throughput sequencing, and differentially expressed circRNAs were verified by quantitative PCR. The gene homology of candidate circRNAs was investigated and the expression of plasma circRNAs from patients with IAV-induced acute respiratory distress syndrome (ARDS) was detected. The target microRNAs (miRNAs) of circRNAs were predicted. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. In total, 781 circRNAs were differentially expressed between ARDS mice and control (467 were up-regulated and 314 were down-regulated). Moreover, the candidate circRNAs (Slco3a1, Nfatc2, Wdr33, and Dmd) expression showed the same trend with the sequencing results. The isoforms of circRNA Slco3a1 and Wdr33 were highly conserved between humans and mice. Plasma circRNA Slco3a1 and Wdr33 presented differential expression in patients with IAV-induced ARDS compared to control. The circRNAmiRNA interaction network and GO and KEGG analyses indicated the potential biological role of circRNAs in the development of IAV-induced lung injury. Taken together, a large number of differentially expressed circRNAs were identified in our study. CircRNA Slco3a1 and Wdr33 had significantly different expression in specimens from mice and humans, and showed a potential biological role in IAV-induced lung injury by bioinformatics analysis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060339PMC
January 2021

A Supervised Video Hashing Method Based on a Deep 3D Convolutional Neural Network for Large-Scale Video Retrieval.

Sensors (Basel) 2021 Apr 29;21(9). Epub 2021 Apr 29.

Major of Electrical Engineering and Electronics, Graduate School of Engineering, Kogakuin University, Tokyo 163-8677, Japan.

Recently, with the popularization of camera tools such as mobile phones and the rise of various short video platforms, a lot of videos are being uploaded to the Internet at all times, for which a video retrieval system with fast retrieval speed and high precision is very necessary. Therefore, content-based video retrieval (CBVR) has aroused the interest of many researchers. A typical CBVR system mainly contains the following two essential parts: video feature extraction and similarity comparison. Feature extraction of video is very challenging, previous video retrieval methods are mostly based on extracting features from single video frames, while resulting the loss of temporal information in the videos. Hashing methods are extensively used in multimedia information retrieval due to its retrieval efficiency, but most of them are currently only applied to image retrieval. In order to solve these problems in video retrieval, we build an end-to-end framework called deep supervised video hashing (DSVH), which employs a 3D convolutional neural network (CNN) to obtain spatial-temporal features of videos, then train a set of hash functions by supervised hashing to transfer the video features into binary space and get the compact binary codes of videos. Finally, we use triplet loss for network training. We conduct a lot of experiments on three public video datasets UCF-101, JHMDB and HMDB-51, and the results show that the proposed method has advantages over many state-of-the-art video retrieval methods. Compared with the DVH method, the mAP value of UCF-101 dataset is improved by 9.3%, and the minimum improvement on JHMDB dataset is also increased by 0.3%. At the same time, we also demonstrate the stability of the algorithm in the HMDB-51 dataset.
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http://dx.doi.org/10.3390/s21093094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124307PMC
April 2021

Intercalation and delamination of TiSnC with high lithium ion storage capacity.

Nanoscale 2021 Apr 13;13(15):7355-7361. Epub 2021 Apr 13.

School of Mechanical and Energy Engineering, Shaoyang University, Shaoyang, Hunan 422000, PR China.

Li-ion batteries attract great attention due to the rapidly increasing and urgent demand for high energy storage devices. MAX phase compounds, layered ternary transition metal carbides and/or nitrides show promise as candidate materials of electrodes for Li-ion batteries. However, the highest specific capacity reported up to now is relatively low (180 mA h g), preventing them from use in real applications. Exploring more MAX phase compounds with delaminated two-dimensional structure is an effective solution to increase the specific capacity. Herein, we report the reversible electrochemical intercalation of Li into TiSnC (MAX phase) nanosheets. Owing to the synergistic effects of intercalation and dimethyl sulfoxide (DMSO)-assisted exfoliation, TiSnC nanosheets are successfully obtained via sonication in DMSO. Moreover, when using as an anode of a Li-ion battery, TiSnC nanosheets exhibited an increasing specific capacity with cycling due to the exfoliation of TiSnC nanosheets via reversible Li-ion intercalation. After 1000 charge-discharge cycles, TiSnC nanosheets delivered a high specific capacity of 735 mA h g at a current density of 50 mA g, which is far better than other MAX phases, such as TiSC, TiSiC and NbSnC. The current work demonstrates the Li-ion storage potential and indicates a novel strategy for further intercalation and delamination of MAX phases.
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http://dx.doi.org/10.1039/d0nr06260jDOI Listing
April 2021

Gentamicin Combined With Hypoionic Shock Rapidly Eradicates Aquaculture Bacteria and .

Front Microbiol 2021 6;12:641846. Epub 2021 Apr 6.

Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China.

Bacterial pathogens are a major cause of infectious diseases in aquatic animals. The abuse of antibiotics in the aquatic industry has led to the proliferation of antibiotic resistance. It is therefore essential to develop more effective and safer strategies to increase the efficacy and extend the life span of the antibiotics used in aquaculture. In this study, we show that six aquaculture bacterial pathogens (i.e., , , , , , and ) in the stationary phase can be rapidly killed after immersion in gentamicin- or neomycin-containing, ion-free solutions for a few minutes. Such hypoionic shock treatment enhances the bacterial uptake of gentamicin in an ATP-dependent manner. Importantly, we demonstrate, as a proof of concept, that gentamicin under hypoionic shock conditions can effectively kill in a skin infection model of zebrafish (), completely curing the infected fish. Given that pathogenic bacteria generally adhere to the skin surface and gills of aquatic animals, our strategy is of potential significance for bacterial infection control, especially for small-scale economic fish farming and ornamental fish farming. Further, the combined treatment can be completed within 5 min with a relatively small volume of solution, thus minimizing the amount of residual antibiotics in both animals and the environment.
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http://dx.doi.org/10.3389/fmicb.2021.641846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055967PMC
April 2021

Rivaroxaban versus low-molecular weight heparin plus warfarin prevents portal vein system thrombosis after splenectomy and pericardial devascularization: A randomized clinical trial.

EXCLI J 2021 4;20:537-549. Epub 2021 Mar 4.

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, Shaanxi 710061, P.R. China.

This study aimed to evaluate the safety and efficacy of rivaroxaban in preventing portal vein system thrombosis (PVST) in patients with liver cirrhosis after splenectomy and pericardial devascularization. 70 cirrhotic patients undergoing splenectomy and pericardial devascularization were randomly assigned to rivaroxaban treatment (n=35) or low-molecular weight heparin (LMWH) plus warfarin treatment (n=35) for 30 days in this randomized controlled trial. The primary endpoint is the PVST formation. Ultrasound doctors and radiologists were blinded to the randomization results. Both groups received routine outpatient inspection every month and were followed for one year. 17 patients (48.6 %) in rivaroxaban group developed PVST, compared with 27 patients (77.1 %) in LMWH plus warfarin group (=0.025). The incidence of PVST during the first year postoperation was significantly lower in rivaroxaban group than in LMWH plus warfarin group (=7.901, =0.006). The intra-group comparisons versus baseline showed the liver function improved from POD 21 to POM 1, and coagulation function improved from POM 2, in rivaroxaban group. In contrast, the liver function improved from POM 1 to POM 2, and coagulation function improved from POM 4, in LMWH plus warfarin group. The prophylactic use of rivaroxaban significantly decreases the incidence of PVST after splenectomy and pericardial devascularization in cirrhotic patients compared to LMWH plus warfarin treatment. Besides, rivaroxaban treatment was safe and effective and associated with better liver and coagulation functions improvement than LMWH plus warfarin treatment.
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http://dx.doi.org/10.17179/excli2020-3120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056059PMC
March 2021

The efficacy of a multistrain probiotic on cognitive function and risk of falls in patients with cirrhosis: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(16):e25535

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong, 250014, China.

Objective: The effect of probiotics on cognitive function and the risk of falling in cirrhosis patients have not been previously evaluated. We perform this protocol for systematic review and meta-analysis to evaluate the effect of a multistrain probiotic on cognitive function and the risk of falls in patients with cirrhosis.

Methods: An all-round retrieval will be performed in 5 electronic journal databases from their inception to March 2021, which comprise Medline, Pubmed, Embase, ScienceDirect, and the Cochrane Library by 2 independent reviewers. Data extraction was performed independently, and any conflict was resolved before final analysis. Only randomized clinical trials were included in this study. The main endpoints were cognitive function and risk of falls, and the secondary endpoints were fall incidence, health-related quality of life (HRQOL), systemic inflammatory response, gut barrier, bacterial translocation, and fecal microbiota. The risk of bias assessment of the included studies was performed by 2 authors independently using the tool recommended in the Cochrane Handbook for Systematic Reviews of Interventions.

Results: We hypothesized that the multistrain probiotic improved cognitive function, risk of falls, and inflammatory response in patients with cirrhosis and cognitive dysfunction.

Conclusion: This study expects to provide credible and scientific clinical evidence for the efficacy and safety of a multistrain probiotic on cognitive function and the risk of falls in patients with cirrhosis.

Osf Registration Number: 10.17605/OSF.IO/JKMTP.
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http://dx.doi.org/10.1097/MD.0000000000025535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078332PMC
April 2021

Overexpression Prox1 in HemECs resembles Kaposiform hemangioendothelioma and cytotoxicity of sirolimus in vitro.

J Pediatr Surg 2021 Jul 26;56(7):1203-1210. Epub 2021 Mar 26.

Department of Pediatric Surgery, Children's Hospital of Fudan University, National Children's Medical Center, 399 Wanyuan Road, Shanghai 201102, China. Electronic address:

Background: Kaposiform hemangioendothelioma (KHE) is a rare vascular tumor that occurs in children. Prox1 is a specific lymphatic marker for KHE. We intended to establish a Prox1 transgenic cell line resembling KHE and investigate the mechanism of sirolimus in treating KHE.

Methods: Prox1 was stably expressed in infantile hemangioma cell HemECs. RT-qPCR and Western blot were conducted to measure the expression of target genes. CCK-8, EdU assay, and cell cycle analysis were conducted to detect cell proliferation. Wound healing and transwell assay were used to evaluate cell migration and invasion.

Results: Both mRNA and protein levels of Prox1, LYVE-1, Podoplanin were upregulated in Prox1 HemECs. An acceleration of cell growth and a rise in migration and invasion were observed with Prox1 overexpression. Sirolimus inhibited cell proliferation, promoted apoptosis and led to G1 phase arrest in Prox1 HemECs. The expression of p-mTOR, p-4EBP1, and p-P70S6K decreased and the ratio of LC-3 II/LC-3 I elevated after treatment of sirolimus.

Conclusions: Stable overexpression of Prox1 in HemECs induced a lymphatic endothelial reprogramming, and enhanced aggressive biological effects, partly resembled the invasion of KHE, and could serve as a novel model for KHE. Sirolimus may block mTOR-mediated pathways and induced autophagy in KHE.
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http://dx.doi.org/10.1016/j.jpedsurg.2021.03.023DOI Listing
July 2021

Finger Citron Extract Ameliorates Glycolipid Metabolism and Inflammation by Regulating GLP-1 Secretion via TGR5 Receptors in Obese Rats.

Evid Based Complement Alternat Med 2021 27;2021:6623379. Epub 2021 Mar 27.

Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China, Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou 510006, China.

Finger citron (FC) is one of many traditional Chinese herbs that have been used to treat obesity. The aim of this study was to elucidate the pharmacological effects and mechanisms of FC on obese rats. Rats were fed with a high-fat diet as a model of obesity and treated with FC at three different dosages for 6 weeks. Pathology in liver tissue was observed. Glucose levels, lipids levels, and inflammatory indicators in serum were evaluated by enzyme-linked immunosorbent assay. Furthermore, the expression of protein-coupled receptor 5 (TGR5) pathway genes in rat colon tissue was detected by reverse transcription-polymerase chain reaction analysis (RT-PCR). Our result revealed that FC alleviates obesity by reducing body weight (BW) and waist circumference, managing inflammation and improving glycolipid metabolism, liver function, and liver lipid peroxidation in vivo. In addition, the mechanism of FC on obesity is possibly the stimulation of glucagon-like peptide-1 (GLP-1) secretion by activating the TGR5 pathway in intestinal endocrine cells. Our studies highlight the obesity reduction effects of FC and one of the mechanisms may be the activation of the TGR5 pathway in intestinal endocrine cells.
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http://dx.doi.org/10.1155/2021/6623379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021467PMC
March 2021

An experimental system to evaluate impact shear failure of rock discontinuities.

Rev Sci Instrum 2021 Mar;92(3):034501

School of Geosciences, University of Edinburgh, Edinburgh EH9 3FE, United Kingdom.

Conventionally, the evaluation of shear failure of discontinuities in rocks and other geomaterials has been conducted under static shear loading. In such methods, the shear failure behaviors of rock discontinuities are significantly influenced by loading velocities. To evaluate the shear failure process under dynamic loading, in this paper, we propose a new experimental methodology by taking advantages of recently available high-speed optical and mechanical measurement techniques. The methodology utilizes the Hopkinson bar to apply impact loading, and the diagnostics include a dynamic stress wave acquisition system, a digital image correlation (DIC) system, and an acoustic emission (AE) monitoring system. To improve the accuracy of the DIC analysis, an advanced digital speckle pattern and an updated water transfer printing are used to obtain the optimized and consistent speckle pattern. A flexible piezoelectric film sensor is first introduced to acquire AE signals in order to locate AE events accurately. A dynamic impact shear experiment indicates that the normal stress has a significant effect on the peak shear stress of rock discontinuities and the peak shear stress itself is rate dependent. The displacement field along shear directions is quantified using the DIC method, and the initial AE source locations during the impact shear process are determined using the AE monitoring system. We thus conclude that the dynamic impact shear system can systematically characterize the dynamic impact shear process with quantitative details and can further be implemented to study other dynamic impact failure behaviors of rock discontinuities under in situ stresses.
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http://dx.doi.org/10.1063/5.0032003DOI Listing
March 2021

Functional photoacoustic/ultrasound imaging for the assessment of breast intraductal lesions: preliminary clinical findings.

Biomed Opt Express 2021 Mar 3;12(3):1236-1246. Epub 2021 Feb 3.

Department of Ultrasound, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

This study aimed to identify features of breast intraductal lesions in photoacoustic/ultrasound (PA/US) imaging and compare PA/US with color Doppler flow/ultrasound (CDFI/US) in the evaluation of breast intraductal lesions. In the nine patients with 10 breast intraductal lesions and 8 patients with 8 benign lesions, total vessel scores evaluated from PA/US are significantly greater than those from CDFI/US (p=0.005). PA internal vessel scores and oxygen saturation (SO) score are significantly increased in breast intraductal lesions than in benign lesions (p=0.016, p=0.006). With a cutoff PA score (sum of PA internal vessel score and SO score) of 2.5, we obtained a sensitivity of 90% and a specificity of 87.5% in differentiation of two groups. PA/US upgraded 40% of breast intraductal lesions, and downgraded 50% of benign lesions from the Breast Imaging Reporting and Data System grading results based on CDFI/US. PA/US functional imaging has the potential in differentiating breast intraductal lesions.
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http://dx.doi.org/10.1364/BOE.411215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984794PMC
March 2021

Clinical effectiveness of fish oil on arterial stiffness: A systematic review and meta-analysis of randomized controlled trials.

Nutr Metab Cardiovasc Dis 2021 05 23;31(5):1339-1348. Epub 2021 Jan 23.

Department of Cardiology, Children's Hospital of Soochow University, Suzhou, Jiangsu, China, University, Suzhou, Jiangsu, China. Electronic address:

Aims: The increase of arterial stiffness is an independent risk factor for cardiovascular diseases (CVD). Fish oil supplementation was shown to reduce the risk of CVD outcomes. However, the effects of fish oil on arterial stiffness remains controversial. This meta-analysis summarized existing randomized clinical trials (RCTs) to determine whether fish oil can affect arterial stiffness in adults.

Data Synthesis: Systematic searches were performed using the PubMed/Medline, EMbase, Cochrane database, Clinical trials, and Web of Science. All RCTs assessed the effect of fish oil intervention on carotid to femoral-Pulse Wave Velocity (cf-PWV), brachial to ankle-PWV (ba-PWV), augmentation index (AIx) and AIx75 were considered. A fixed-effect model was used to calculate the pooled effect. A total of 14 RCTs were included. The pooled data analysis showed that fish oil significantly reduced PWV levels (SMD: -0.145, 95%CI: -0.265 to -0.033, P = 0.012) compared to the control group. In subgroup analyses, a significant decrease in PWV was found in trials that fish oil with low dosages (≤1.8 g/d), short time (<24 weeks), low DHA to EPA ratio (DHA/EPA<1) and among young participant (<50 years old). Besides, the effect of fish oil was more obvious in ba-PWV compared to cf-PWV. In contrast, the effect of fish oil supplementation on AIx (WMD: -0.588%, 95% CI: -2.745 to 1.568, P = 0.593) and AIx75 (WMD: 0.542%, 95% CI: -1.490 to 2.574, P = 0.601) was nonsignificant.

Conclusions: The current study showed that fish oil supplementation had a beneficial effect on pulse wave velocity.
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http://dx.doi.org/10.1016/j.numecd.2020.12.033DOI Listing
May 2021

Prevalence and risk factor analysis for the nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus.

Medicine (Baltimore) 2021 Mar;100(10):e24940

Experimental Center of Clinical Research, The First Affiliated Hospital of Anhui University of Chinese Medicine.

Abstract: Although non-alcoholic fatty liver disease (NAFLD) is strongly associated with type 2 diabetes mellitus (T2DM), the diagnosis of NAFLD for T2DM patients remains a challenge.This study aimed to investigate the prevalence and risk factors for the NAFLD in T2DM outpatients.This is a retrospective, cross-sectional study that included 2405 T2DM patients treated and admitted for glucose control into the Endocrinology Department of our hospital from April 2017 to March 2019. Using strict exclusion criteria, the target patients were screened and divided into two groups: NAFLD patients (study group) and non-NAFLD patients (control group). Subsequently, 34 factors were compared between the two groups. Furthermore, multivariate analysis of the NAFLD risk factors was performed using logistic regression. Finally, the diagnostic significance of individual biochemical predictors, as well as the combined predictive indicator (CPI), for NAFLD was estimated using receiver operating characteristic (ROC) curve analysis.In this study, the overall prevalence of NAFLD in T2DM patients was 58.67%. Of the target patients, 17 factors were identified by univariate analysis to be associated with NAFLD, and 8 factors were found to be significant predictors for NAFLD using binary logistic regression modeling. Furthermore, the CPI and C-Peptide represent high diagnostic value for NAFLD in T2DM patients.This study provides a more comprehensive risk factor analysis for NAFLD in T2DM patients. These data can be used to provide timely diagnosis and effective management of NAFLD.
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http://dx.doi.org/10.1097/MD.0000000000024940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969325PMC
March 2021

Lacunar-like changes of the chorion: can it be a first-trimester ultrasound sign in predicting worse clinical outcome in cesarean scar pregnancy termination?

J Matern Fetal Neonatal Med 2021 Jul 8;34(14):2355-2362. Epub 2021 Mar 8.

Department of Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Objective: To observe the lacunar-like changes in cesarean scar pregnancy (CSP) ultrasonography in first trimester and to explore its relationship with clinical outcome in early pregnancy termination.

Methods: This is a retrospective case-control study. Patients who were diagnosed as CSP and chose to terminate pregnancy from January 2017 to April 2020 were enrolled. According to occurrence of lacunar-like change in chorion membrane, patients were divided into case and control group. The clinical manifestation, laboratory test, ultrasound data, and outcome were compared.

Results: Fifty-five CSP patients were enrolled with 20 (36.4%) in case group and 35 (63.6%) in control group. As for ultrasound features, the maximum outer diameter of gestational mass (5.6 ± 2.5 cm vs. 3.9 ± 1.5 cm), the maximum thickness of the chorion membrane (median number 1.1 cm vs. 0.7 cm), the longitudinal diameter of the implanting part of gestational mass in uterine lower segment (3.3 ± 1.8 cm vs. 1.2 ± 0.5 cm), uterine lower segment protrusion incidence (12, 60% vs. 2, 5.7%), and the crown-rump length of fetus (median number 1.7 cm vs. 0.7 cm) were bigger or higher in case group than that of the control group; the minimum thickness of the uterine lower segment myometrium (median number 0.08 cm vs. 0.20 cm) was significantly thinner in case group. CDFI grading of case group was different from control group with more cases in higher grades. As for clinical outcome, the patients of case group showed more frequency of CSP lesion resection under open surgery or laparoscopy (7, 35% vs. 1, 2.86%) rather than suction curettage, more blood loss in surgery (median number 35 ml vs. 20 ml) and more hospitalization days (median number 7.5 d vs. 3.5 d) than control group.

Conclusions: Lacunar-like change of chorion can be detected in early gestation and may act as a predictor of complicated and worse clinical outcome.
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http://dx.doi.org/10.1080/14767058.2021.1888914DOI Listing
July 2021

Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodents.

Transl Psychiatry 2021 02 24;11(1):140. Epub 2021 Feb 24.

Department of Physiology, School of Medicine, Jinan University, 510632, Guangzhou, China.

The transcription factor erythroid 2-related factor 2 (Nrf2) and brain-derived neurotrophic factor (BDNF) play a key role in depression. However, the molecular mechanisms underlying the crosstalk between Nrf2 and BDNF in depression remain unclear. We examined whether Nrf2 regulates the transcription of Bdnf by binding to its exon I promoter. Furthermore, the role of Nrf2 and BDNF in the brain regions from mice with depression-like phenotypes was examined. Nrf2 regulated the transcription of Bdnf by binding to its exon I promoter. Activation of Nrf2 by sulforaphane (SFN) showed fast-acting antidepressant-like effects in mice by activating BDNF as well as by inhibiting the expression of its transcriptional repressors (HDAC2, mSin3A, and MeCP2) and revising abnormal synaptic transmission. In contrast, SFN did not affect the protein expression of BDNF and its transcriptional repressor proteins in the medial prefrontal cortex (mPFC) and hippocampus, nor did it reduce depression-like behaviors and abnormal synaptic transmission in Nrf2 knockout mice. In the mouse model of chronic social defeat stress (CSDS), protein levels of Nrf2 and BDNF in the mPFC and hippocampus were lower than those of control and CSDS-resilient mice. In contrast, the protein levels of BDNF transcriptional repressors in the CSDS-susceptible mice were higher than those of control and CSDS-resilient mice. These data suggest that Nrf2 activation increases the expression of Bdnf and decreases the expression of its transcriptional repressors, which result in fast-acting antidepressant-like actions. Furthermore, abnormalities in crosstalk between Nrf2 and BDNF may contribute to the resilience versus susceptibility of mice against CSDS.
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http://dx.doi.org/10.1038/s41398-021-01261-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904924PMC
February 2021

Comparison of efficacy and safety of corticosteroid and vincristine in treating kaposiform hemangioendothelioma and tufted angioma: A multicenter prospective randomized controlled clinical trial.

J Dermatol 2021 May 19;48(5):576-584. Epub 2021 Feb 19.

Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.

Kaposiform haemangioendothelioma (KHE) and tufted angioma (TA) are rare vascular tumors that can cause life-threatening Kasabach-Merritt phenomenon. No evidence-based treatment strategies have yet been established, and its management is still a challenge. The purpose of this multicenter prospective randomized controlled study was to evaluate and compare the efficacy of corticosteroid and vincristine (VCR) in the treatment of KHE and TA. All patients with KHE/TA who met the diagnostic criteria were consecutively recruited. The patients were randomized into a methylprednisolone (MP) group and a VCR group. The primary outcome was the single main parameter effective rate and overall effective rate of corticosteroid and VCR over 1 month after treatment. The single main parameters included platelets, fibrinogen, tumor size, texture, and appearance. From May 2016 to April 2018, a total of 59 patients completed the clinical trial, including 29 in the MP group and 30 in the VCR group. The results showed that VCR was superior to corticosteroid in the improvement of platelet (80.0% vs 44.0%, P = 0.019) and tumor texture (68.9% vs 30.8%, P = 0.007). Although the efficacy of VCR on fibrinogen (23.3% vs 20.7%, P = 1.000), tumor size (23.3% vs 13.8%, P = 0.273), and appearance (65.5% vs 46.2%, P = 0.120) was higher than that of corticosteroid, there was no significant difference (P > 0.05). Meanwhile, the overall effective rate of VCR was higher than that of corticosteroid (56.7% vs 31.0%), but the difference was also not statistically significant (P = 0.067). In conclusion, the therapeutic effect of VCR was significantly better than that of corticosteroid with regard to treating thrombocytopenia and tumor texture. We recommend that VCR could be an option for first-line treatment in KHE/TA patients.
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http://dx.doi.org/10.1111/1346-8138.15767DOI Listing
May 2021

Autonomous Robot for Removing Superficial Traumatic Blood.

IEEE J Transl Eng Health Med 2021;9:2600109. Epub 2021 Feb 2.

Department of NeurosurgeryXuanwu HospitalCapital Medical UniversityBeijing100053China.

: To remove blood from an incision and find the incision spot is a key task during surgery, or else over discharge of blood will endanger a patient's life. However, the repetitive manual blood removal involves plenty of workload contributing fatigue of surgeons. Thus, it is valuable to design a robotic system which can automatically remove blood on the incision surface. : In this paper, we design a robotic system to fulfill the surgical task of the blood removal. The system consists of a pair of dual cameras, a 6-DoF robotic arm, an aspirator whose handle is fixed to a robotic arm, and a pump connected to the aspirator. Further, a path-planning algorithm is designed to generate a path, which the aspirator tip should follow to remove blood. : In a group of simulating bleeding experiments on ex vivo porcine tissue, the contour of the blood region is detected, and the reconstructed spatial coordinates of the detected blood contour is obtained afterward. The BRR robot cleans thoroughly the blood running out the incision. : This study contributes the first result on designing an autonomous blood removal medical robot. The skill of the surgical blood removal operation, which is manually operated by surgeons nowadays, is alternatively grasped by the proposed BRR medical robot.
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http://dx.doi.org/10.1109/JTEHM.2021.3056618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880304PMC
February 2021

Investigation of Dropwise Condensation on a Super-Aligned Carbon Nanotube Mesh-Coated Surface.

Langmuir 2021 Mar 14;37(8):2629-2638. Epub 2021 Feb 14.

Department of Space Science Research, Qian Xuesen Laboratory of Space Technology, China Academy of Space Technology, Beijing 100094, China.

Enhanced vapor condensation is a critical issue for improving the efficiency of energy conversion, thermal management, water recovery, and treatment. Low-energy surfaces incorporating micro/nanoscale roughness have been reported to significantly promote vapor condensation. In this research, the mesh structures of super-aligned carbon nanotube (SACNT) films were prepared by crossing monolayer SACNT films on a copper substrate. Then, the sustaining dropwise condensation was achieved on the SACNT mesh-coated surface. The SACNT mesh-coated surface could obviously enhance the coalescence and sweeping departure of the condensing droplets. Additionally, the measured overall heat transfer coefficient (HTC) of the SACNT mesh-coated surface demonstrated a 36% enhancement compared to that on the bare copper surface. The parallel stacking of SACNT films with different groove structures was also studied, and a 15% enhancement in the HTC was shown as compared with the bare copper surface. Furthermore, we developed a morphology-based model to theoretically analyze the condensation-enhancement mechanism on a SACNT mesh-coated surface. The SACNT surfaces also have advantages of low cost, durability, flexibility, and extensibility. Our findings revealed that the SACNT films could be readily used as vapor condensation-strengthening surfaces, further extending their potential applications to industrial equipment.
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http://dx.doi.org/10.1021/acs.langmuir.0c03263DOI Listing
March 2021

Interactions between ScNAC23 and ScGAI regulate GA-mediated flowering and senescence in sugarcane.

Plant Sci 2021 Mar 25;304:110806. Epub 2020 Dec 25.

State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi University, Nanning, 530005, China; Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning, 530005, China. Electronic address:

Control of gene transcription is crucial to regulate plant growth and development events, such as flowering, leaf senescence, and seed germination. Here we identified a NAC transcription factor (ScNAC23) isolated from sugarcane (cv. ROC22). Analysis by qRT-PCR indicated that ScNAC23 expression was strongly induced in mature leaves and flowering varieties and was also responsive to exogenous treatment with the hormone gibberellin (GA). Ectopic expression of ScNAC23 in Arabidopsis accelerated bolting, flowering, and leaf senescence compared to wild type plants. Furthermore, Arabidopsis overexpressed ScNAC23 were more sensitive to GA than the wild type, and exogenous GA significantly accelerated flowering and senescence in the ScNAC23-overexpressed ones. A direct interaction between ScNAC23 and ScGAI, an inhibitor of GA signaling, was confirmed by yeast-two hybrid, bimolecular fluorescence complementation, and GST-pull down assay. The putative GA-ScNAC23-LFY/SAGs regulator module might provide a new sight into the molecular action of GA to accelerating flowering and leaf senescence in sugarcane.
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http://dx.doi.org/10.1016/j.plantsci.2020.110806DOI Listing
March 2021

The formation and evolution of centromeric satellite repeats in Saccharum species.

Plant J 2021 May 9;106(3):616-629. Epub 2021 Mar 9.

Guangxi Key Laboratory of Sugarcane Biology & Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops (MOE), College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Centromeres in eukaryotes are composed of tandem DNAs and retrotransposons. However, centromeric repeats exhibit considerable diversity, even among closely related species, and their origin and evolution are largely unknown. We conducted a genome-wide characterization of the centromeric sequences in sugarcane (Saccharum officinarum). Four centromeric tandem repeat sequences, So1, So103, So137 and So119, were isolated. So1 has a monomeric length of 137 bp, typical of a centromeric satellite, and has evolved four variants. However, these So1 variants had distinct centromere distributions and some were unique to an individual centromere. The distributions of the So1 variants were unexpectedly consistent among the Saccharum species that had different basic chromosome numbers or ploidy levels, thus suggesting evolutionary stability for approximately 7 million years in sugarcane. So103, So137 and So119 had unusually longer monomeric lengths that ranged from 327 to 1371 bp and lacked translational phasing on the CENH3 nucleosomes. Moreover, So103, So137 and So119 seemed to be highly similar to retrotransposons, which suggests that they originated from these mobile elements. Notably, all three repeats were flanked by direct repeats, and formed extrachromosomal circular DNAs (eccDNAs). The presence of circular molecules for these retrotransposon-derived centromeric satellites suggests an eccDNA-mediated centromeric satellite formation pathway in sugarcane.
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http://dx.doi.org/10.1111/tpj.15186DOI Listing
May 2021

Genomic Characterization of WRKY Transcription Factors Related to Andrographolide Biosynthesis in .

Front Genet 2020 18;11:601689. Epub 2021 Jan 18.

Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.

Andrographolide, which is enriched in the leaves of , has been known as "natural antibiotic" due to its pharmacological activities such as anti-inflammatory, antimicrobial and antioxidant effects. Several key enzymes in andrographolide biosynthetic pathway have been studied since the genome sequences were released, but its regulatory mechanism remains unknown. WRKY transcription factors proteins have been reported to regulate plant secondary metabolism, development as well as biotic and abiotic stresses. Here, WRKY transcription factors related to andrographolide biosynthesis were systematically identified, including sequences alignment, phylogenetic analysis, chromosomal distribution, gene structure, conserved motifs, synteny, alternative splicing event and Gene ontology (GO) annotation. A total of 58 WRKYs were identified in Chuanxinlian genome and phylogenetically classified into three groups. Moreover, nine WRKY genes underwent alternative splicing events. Furthermore, the combination of binding site prediction, gene-specific expression patterns, and phylogenetic analysis suggested that 7 WRKYs (, and ) might regulate andrographolide biosynthesis. This study laid a foundation for understanding the regulatory mechanism of andrographolide biosynthesis and the improvement and breeding of varieties.
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http://dx.doi.org/10.3389/fgene.2020.601689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848199PMC
January 2021

BiNV bond: A hole-transfer bridge for high-efficient separation and transfer of carriers.

J Colloid Interface Sci 2021 May 21;590:144-153. Epub 2021 Jan 21.

College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; School of Mechanical Engineering, University of Western Australia, 35 Stirling Highway, WA 6009, Australia. Electronic address:

Addressing the inherent holes transport limitation of BiVO photoanode is crucial to achieve efficient photoelectrochemical (PEC) water splitting. The construction of the hole-transfer bridge between co-catalysts and BiVO photoanode could be an effective way to overcome sluggish hole-transfer kinetics of BiVO photoanode. Herein, CN/BiVO photoanode was prepared by coupling carbon nitride hydrogel (CNH) containing unsaturated N on the BiVO photoanode during annealing. CN/BiVO photoanode exhibited excellent PEC performance and stability. Photoelectrochemical tests proved that the coupling of CN accelerated holes transfer and enhanced oxygen evolution kinetics. X-ray photoelectron spectroscopy (XPS) and theoretical calculations confirmed the existence of the BiNV bond between BiVO photoanode and CN, which could serve as the hole-transfer bridge to significantly accelerate separation and transfer of carriers driven by the interfacial electric field. Moreover, it was found that the coupling of CN effectively inhibited the dissociation of metal ions through changing their coordination environment, resulting in the excellent stability of CN/BiVO photoanode. This result provides unique insights into vital roles of the interfacial structure, which might have a significant impact on the construction of PEC devices.
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http://dx.doi.org/10.1016/j.jcis.2021.01.043DOI Listing
May 2021