Publications by authors named "Wei Xiong"

1,465 Publications

  • Page 1 of 1

Correction to: Natural product triptolide induces GSDME-mediated pyroptosis in head and neck cancer through suppressing mitochondrial hexokinase-ΙΙ.

J Exp Clin Cancer Res 2021 Sep 22;40(1):298. Epub 2021 Sep 22.

Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Tongzipo Road, Changsha, 410013, Hunan, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13046-021-02100-8DOI Listing
September 2021

Correction to: Pyroptosis: a new paradigm of cell death for fighting against cancer.

J Exp Clin Cancer Res 2021 Sep 22;40(1):296. Epub 2021 Sep 22.

Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Tongzipo Road, Changsha, 410013, Hunan, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13046-021-02101-7DOI Listing
September 2021

Effects of Sevoflurane-Propofol-Balanced Anesthesia on Flash Visual Evoked Potential Monitoring in Spine Surgery: A Randomized Noninferiority Trial.

Anesth Analg 2021 Sep 20. Epub 2021 Sep 20.

From the Department of Anesthesiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.

Background: Intraoperative flash visual evoked potential (FVEP) can be used to monitor visual function during spine surgery. However, it is limited due to the previous perception of its sensitivity to inhalation anesthesia. We conducted this trial to test the noninferiority of sevoflurane-propofol-balanced anesthesia (BA) versus popular propofol-based total intravenous anesthesia (TIVA) on the amplitude of FVEP during spine surgery.

Methods: A total of 60 patients undergoing spine surgery were randomized to receive either sevoflurane-propofol-balanced anesthesia (BA group) or propofol-based total intravenous anesthesia (TIVA group) for anesthesia maintenance. We titrated the propofol plasma concentration to keep the bispectral index (BIS) values between 40 and 50. The primary outcome was the P100-N145 amplitudes of FVEP at 120 minutes after induction of anesthesia. The noninferiority margin (δ) was defined as 10% of the P100-N145 amplitude at 120 minutes after induction in the TIVA group. If the confidence interval (CI) for mean differences of P100-N145 amplitude at 120 minutes after induction between BA and TIVA groups lied above the lower limit of -δ with P < .025, we defined BA group was noninferior to TIVA group.

Results: Fifty-nine patients were included in the final analysis. The amplitude of P100-N145 at 120 minutes after anesthesia induction in group BA was noninferior to group TIVA (3.8 [1.3] µV vs 3.2 [1.6] µV, -δ = -0.32, mean difference, 0.57, 95% CI, -0.18 to 1.33, P for noninferiority = .015).

Conclusions: The effect of 0.5 minimum alveolar concentration (MAC) of sevoflurane-propofol-balanced anesthesia on the P100-N145 amplitude of FVEP was noninferior to that of propofol-based TIVA under comparable BIS range.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1213/ANE.0000000000005742DOI Listing
September 2021

IL-39 increases ROS production and promotes the phosphorylation of p38 MAPK in the apoptotic cardiomyocytes.

Folia Histochem Cytobiol 2021 Sep 20. Epub 2021 Sep 20.

Department of Gerontology, Shenzhen People's Hospital, The Second Affiliated Hospital of Jinan Medical College, Shenzhen, China.

Introduction: The cytokine interleukin (IL)-39 is a novel member of the IL-12 family. Our previous study found that the serum level of IL-39 significantly increased in patients with acute myocardial infarction. However, the role of IL-39 in cardiomyocyte apoptosis remains unclear.

Material And Methods: In this study, the cultured mouse HL-1 cardiomyocytes were incubated with PBS, 0-100 ng/mL IL-39, 200 μM H₂O₂ or 20 μM Trolox.

Results: IL-39 promoted the production of intracellular reactive oxygen species (ROS) in a concentration-dependent manner in HL-1 cardiomyocytes. IL-39 and H₂O₂ both significantly promoted the production of intracellular ROS, increased the level of intracellular CCL2, stimulated the apoptotic progress of cardiomyocytes, increased the mRNA and protein expression levels of Bax, caspase-3, and p-p38 MAPK, and decreased the mRNA and protein expression levels of Bcl-2. ROS production, CCL2 level, cardiomyocyte apoptosis, and expression of Bax, caspase-3, and p-p38 MAPK were significantly amplified by the administration of IL-39 combined with H₂O₂, and these processes were significantly alleviated by an antioxidant Trolox.

Conclusion: This study was novel in revealing that IL-39 promoted apoptosis by stimulating the phosphorylation of p38 MAPK in mouse HL-1 cardiomyocytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5603/FHC.a2021.0019DOI Listing
September 2021

Interaction screening for high-dimensional heterogeneous data via robust hybrid metrics.

Authors:
Wei Xiong Han Pan

Stat Med 2021 Sep 20. Epub 2021 Sep 20.

School of Statistics, University of International Business and Economics, Beijing, China.

A novel model-free interaction screening approach called the hybrid metrics is introduced for high-dimensional heterogeneous data analysis. The metrics established based on the variation of conditional joint distribution function are measurements of interaction that include both size and direction. They are robust and can work with many types of response variables, including continuous, discrete, and categorical variables. We can apply the hybrid metrics to effective interaction selection for classification, response index models, and Poisson regression, among others. When dealing with classification, the hybrid metrics are capable of capturing both nonlinear category-general and category-specific interaction effects, providing us with a comprehensive overview and precise discovery of category information. When faced with a continuous response, the hybrid metrics perform fairly well even if the signal strength is weak, behaving as if the true interactions were known. To facilitate implementation, a fast two-stage procedure which naturally and efficiently enforces both strong and weak heredity is advocated. We further demonstrate their superior performances over popular competitors by exhaustive simulations and a SRBCT real data example. Supplementary materials for this article are available online.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/sim.9204DOI Listing
September 2021

Epigenetics effect on pathogenesis of thyroid-associated ophthalmopathy.

Int J Ophthalmol 2021 18;14(9):1441-1448. Epub 2021 Sep 18.

Department of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China.

Thyroid-associated ophthalmopathy (TAO) is an autoimmune disease. Recent studies have found the aberrant epigenetics in TAO, including DNA methylation, non-coding RNAs, and histone modification. Many genes have an aberrant level of methylation in TAO. For example, higher levels are found in CD14, MBP, ANGLE1, LYAR and lower levels in DRD4 and BOLL. Non-coding RNAs are involved in the immune response (miR-146a, miR-155, miR-96, miR-183), fibrosis regulation (miR-146a, miR-21, miR-29), adipogenesis (miR-27) and are thought to play roles in TAO. MicroRNA is also related to the clinical activity score (miR-Let7d-5p) and may be a predictor of glucocorticoid therapy (miR-224-5p). The quantities of H4 in TAO are increased compared with euthyroid control subjects, and the role of histone modifications in Graves' disease may lead to better understanding of its role in TAO. More studies are needed to explain the role of epigenetics in TAO and provide potential therapeutic strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18240/ijo.2021.09.22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403864PMC
September 2021

The regulatory networks of the Hippo signaling pathway in cancer development.

J Cancer 2021 28;12(20):6216-6230. Epub 2021 Aug 28.

NHC Key Laboratory of Carcinogenesis, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.

The Hippo signaling pathway is a relatively young tumor-related signaling pathway. Although it was discovered lately, research on it developed rapidly. The Hippo signaling pathway is closely relevant to the occurrence and development of tumors and the maintenance of organ size and other biological processes. This manuscript focuses on YAP, the core molecule of the Hippo signaling pathway, and discussion the upstream and downstream regulatory networks of the Hippo signaling pathway during tumorigenesis and development. It also summarizes the relevant drugs involved in this signaling pathway, which may be helpful to the development of targeted drugs for cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.62402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425214PMC
August 2021

The role of Ca /Calcineurin/NFAT signalling pathway in osteoblastogenesis.

Cell Prolif 2021 Sep 15:e13122. Epub 2021 Sep 15.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

The bone remodelling process is closely related to bone health. Osteoblasts and osteoclasts participate in the bone remodelling process under the regulation of various factors inside and outside. Excessive activation of osteoclasts or lack of function of osteoblasts will cause occurrence and development of multiple bone-related diseases. Ca /Calcineurin/NFAT signalling pathway regulates the growth and development of many types of cells, such as cardiomyocyte differentiation, angiogenesis, chondrogenesis, myogenesis, bone development and regeneration, etc. Some evidences indicate that this signalling pathway plays an extremely important role in bone formation and bone pathophysiologic changes. This review discusses the role of Ca /Calcineurin/NFAT signalling pathway in the process of osteogenic differentiation, as well as the influence of regulating each component in this signalling pathway on the differentiation and function of osteoblasts, whereby the relationship between Ca /Calcineurin/NFAT signalling pathway and osteoblastogenesis could be deeper understood.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cpr.13122DOI Listing
September 2021

Editorial: Microbial C1 Metabolism and Biotechnology.

Front Microbiol 2021 27;12:744030. Epub 2021 Aug 27.

Department of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, United States.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.744030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429839PMC
August 2021

Deletion of Causes Hearing Loss and Abnormal Auditory Nerve Fibers in the Mouse Cochlea.

Front Cell Neurosci 2021 25;15:713651. Epub 2021 Aug 25.

Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Complement C1q Like 1 (C1QL1), a secreted component of C1Q-related protein, is known to play an important role in synaptic maturation, regulation, and maintenance in the central nervous system. is expressed in adult cochlear inner and outer hair cells (IHCs and OHCs) with preferential expression in OHCs. We generated null mice to examine the role of C1QL1 in the auditory periphery. -null mice exhibited progressive hearing loss with elevated thresholds of auditory brainstem response and distortion product otoacoustic emission. Confocal microscopy showed that the number of nerve fibers innervating both IHCs and OHCs was significantly reduced. However, spiral ganglion neurons appeared to be normal under electron microscopy. IHC development and survival were not affected by deletion of Voltage-clamp recording and immunocytochmistry combined with confocal microscopy showed -null IHCs showed no significant reduction of pre-synaptic proteins and synaptic vesicle release. This is in contrast to significant OHC loss in the KO mice. Our study suggests that is essential for development of hair cell innervation and OHC survival. But maturation of presynaptic machinery in IHCs does not depend on C1QL1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fncel.2021.713651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424102PMC
August 2021

Milrinone is better choice for controlled low central venous pressure during hepatectomy: A randomized, controlled trial comparing with nitroglycerin.

Int J Surg 2021 Sep 6;94:106080. Epub 2021 Sep 6.

The First Affiliated Hospital of Sun Yat-sen University, China. Electronic address:

Background: To explore the feasibility and effectiveness of controlled low central venous pressure(CLCVP)induced by milrinone during hepatectomy, and its influence on perioperative hemodynamics, blood loss and patients' recovery, comparing with the traditional method by nitroglycerin.

Methods: 52 patients who underwent elective open hepatectomy were enrolled in the study and randomly divided into two groups: milrinone (M) group and nitroglycerin (NG) group. Milrinone was infused with the rate of 0.5 μg/kg/min in group M, while nitroglycerin was given 0.2-0.5 μg/kg/min in group NG to maintain CVP≤ 5 mmHg during liver resection. The demographic variables, data of the operative procedure and intraoperative hemodynamics were recorded. The postoperative recovery profiles and pre- and post-operative haematological markers of vital organs were also collected and compared.

Results: 1. The blood loss of group M, no matter during liver resection or in the whole procedure, was both less than that of group NG (P < 0.05), so did the hemoglobin detected by blood gas analysis (P < 0.05). Meanwhile, time of hepatectomy and hepatic hilum occlusion were shorter in group M (P < 0.05). 2. Compared with the NG group, cardiac index (CI) and stroke volume index (SVR) were higher in group M in the operation. The norepinephrine dosage necessary in the operation was of no difference in two groups (P > 0.05). 3. Drainage indwelling time and postoperative hospital stay of group M were shorter than that of group NG (P < 0.05). Most of the blood biomarkers increased on postoperative day (POD)-1, and returned to the preoperative level on POD-7 without inter-group difference (P > 0.05). Brain natriuretic peptide precursor (Pro-BNP) in group M was higher than NG group on POD-1 (P < 0.05), and the statistical difference disappeared on POD-7.

Conclusion: Milrinone can effectively maintain a controlled low central venous pressure during hepatectomy. Compared with nitroglycerin, milrinone can reduce the amount of blood loss, with the benefit of better manifestation of hemodynamics and enhanced postoperative recovery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijsu.2021.106080DOI Listing
September 2021

Qisheng Wan formula ameliorates cognitive impairment of Alzheimer's disease rat via inflammation inhibition and intestinal microbiota regulation.

J Ethnopharmacol 2021 Sep 4;282:114598. Epub 2021 Sep 4.

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, PR China. Electronic address:

Ethnopharmacology Relevance: Qisheng Wan formula (QWF) was first described in the book Sheng Ji Zong Lu in 1117. The book states that QWF can cure forgetfulness, improve the mind, and make people smart. Hence, QWF has been widely used to treat patients with forgetfulness or dementia. QWF, a classic Chinese formulation, comprises seven herbal drugs: the sclerotium of Poria cocos (Schw.) Wolf, bark of Cinnamomum cassia Presl, root of Polygala tenuifolia Willd., root and rhizome of Panax ginseng C. A. Mey., root of Asparagus cochinchinensis (Lour.) Merr., root and rhizome of Acorus tatarinowii Schott, and root bark of Lycium chinense Mill.

Aim Of The Study: This study aimed to utilize modern pharmacological methods to evaluate the therapeutic effects and explore the underlying mechanism of QWF action on rats with Alzheimer's disease (AD).

Materials And Methods: The chemical profile of QWF was characterized using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The AD rat model was established via a bilateral intraventricular injection of amyloid-β (1-42) (Aβ). The rats were subsequently treated daily with QWF for 4 weeks. The Morris water maze test was performed to evaluate the cognition processes in the rats, whereas histological changes in the hippocampus were observed using hematoxylin and eosin staining. The expression levels of Aβ, nuclear factor-kappa B (NF-κB), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in the hippocampus and colon were assessed. Moreover, the diversity and composition of the intestinal microbiota were analyzed using 16S rDNA gene sequencing.

Results: One hundred and fourteen compounds were characterized in QWF. QWF significantly ameliorated the cognition processes and histopathological damages due to AD in rats by decreasing the deposition of Aβ and downregulating the expression of NF-κB, TNF-α, and IL-6. QWF also modulated changes in the diversity and composition of intestinal microbiota to suppress the relative abundance of inflammation-associated microbiota.

Conclusion: This study showed that QWF can suppress proinflammatory factors and modulate the intestinal microbiota in AD rats.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2021.114598DOI Listing
September 2021

Effects of temperature on metabolic scaling in silver carp.

J Exp Zool A Ecol Integr Physiol 2021 Sep 7. Epub 2021 Sep 7.

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Sciences, Southwest University, Chongqing, China.

The association between temperature and metabolic scaling varies among species, which could be due to variation in the surface area and its scaling. This study aims to examine the effect of temperature on metabolic scaling and to verify the links between metabolic scaling and surface area scaling at both the whole body and the cell levels. The routine metabolic rate (RMR), gill surface area (GSA), ventilation frequency (VF), red blood cell surface area (S ), and metabolic rate (MR ) were determined in silver carp, and their mass-scaling exponents were analyzed at 10 and 25°C. These results showed that body mass and temperature independently affected the RMR, GSA, and VF, suggesting constant scaling exponents of RMR (0.772), GSA (0.912), and VF (-0.282) with changing temperature. The RMR at 25°C was 2.29 times higher than that at 10°C, suggesting increased metabolic demand at a higher temperature. The results showed that the RMR increased, while the scaling exponents of RMR, GSA, and VF remained unchanged with increasing temperature. These results support the view that the scaling of oxygen supply capacity importantly affects metabolic scaling. The S did not change with either temperature or body mass. However, the MR increased by 5.48 times from 10 to 25°C but did not change with body mass. As the scaling exponents of RMR did not change between temperatures, the results indicate that no obvious link exists between the scaling of both the cell size and cell metabolic rate and the metabolic scaling of silver carp.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jez.2542DOI Listing
September 2021

Current Status of Immune Checkpoint Inhibitor Immunotherapy for Lung Cancer.

Front Oncol 2021 18;11:704336. Epub 2021 Aug 18.

Department of Pulmonary and Critical Care Medicine, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Immunotherapy is a major breakthrough in the treatment of cancer in recent years. Immune checkpoint inhibitors (ICIs) including programmed death-ligand 1 ()/programmed death-1 () and cytotoxic T-lymphocyte antigen-4 () have been used for different histologic types of cancer including primary lung cancer that represents the most common and fatal cancer globally. Among ICI immunotherapy agents, atezolizumab, durvalumab, ipilimumab, nivolumab, and pembrolizumab are currently used as standard-of-care (SOC) treatment for metastatic or earlier stages of lung cancer. Major issues of ICI immunotherapy in lung cancer comprise the use of immune biomarkers prior to ICI therapy, selection of ICI agents, combination of ICIs/chemotherapy, combination of ICIs/radiotherapy, sequence of tyrosine kinase inhibitor (TKI) targeted therapy and ICI immunotherapy, sequence of chemotherapy and ICI immunotherapy, treatment duration of ICI regimen and ICI therapy for different histopathology, stage, , and performance status. Based on the contemporary major clinical trials and authoritative guidelines, the objective of this review is to present an overview of the current status of ICI immunotherapy in lung cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.704336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416501PMC
August 2021

Effective tumor vessel barrier disruption mediated by perfluoro-N-(4-methylcyclohexyl) piperidine nanoparticles to enhance the efficacy of photodynamic therapy.

Nanoscale 2021 Aug 30;13(31):13473-13486. Epub 2021 Jul 30.

Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.

Background: Currently, limited tumor drug permeation, poor oxygen perfusion and immunosuppressive microenvironments are the most important bottlenecks that significantly reduce the efficacy of photodynamic therapy (PDT). The main cause of these major bottlenecks is the platelet activation maintained abnormal tumor vessel barriers. Thus, platelet inhibition may present a new way to most effectively enhance the efficacy of PDT. However, to the best of our knowledge, few studies have validated the effectiveness of such a way in enhancing the efficacy of PDT both in vivo and in vitro. In this study, perfluoro-N-(4-methylcyclohexyl) piperidine-loaded albumin ([email protected]) nanoparticles were discovered, which possess excellent platelet inhibition ability. After [email protected] treatment, remarkably enhanced intra-tumoral drug accumulation, oxygen perfusion and T cell infiltration could be observed owing to the disrupted tumor vessel barriers. Besides, the effect of [email protected] mediated PDT was significantly amplified by [email protected] nanoparticles. It was demonstrated that [email protected] could be used as a useful tool to improve the efficacy of existing PDT by disrupting tumor vessel barriers through effective platelet inhibition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1nr02880dDOI Listing
August 2021

Identification of candidate genes and pathways in limonin-mediated cardiac repair after myocardial infarction.

Biomed Pharmacother 2021 Oct 28;142:112088. Epub 2021 Aug 28.

Department of Clinical Research, Ziyang People's Hospital, Ziyang Hospital of Sichuan Provincial People's Hospital, Ziyang, Sichuan, China; Department of Emergency Intensive Care Unit, Ziyang People's Hospital, Ziyang Hospital of Sichuan Provincial People's Hospital, Ziyang, Sichuan, China. Electronic address:

Background: Myocardial infarction (MI) resulting from acute coronary ischemia may cause significant morbidity and mortality, and microRNAs play a vital role in this pathophysiology. Limonin (LIM) is a natural medicine from citrus fruit that protects organs against ischemic diseases, but the candidate genes and pathways associated with cardioprotection are unknown.

Methods: MI was induced by ligating the left anterior descending coronary in male Sprague-Dawley rats. LIM was orally administered for 7 days after the induction of MI. Subsequently, the hearts were collected to examine significant changes in microRNAs and mRNAs among the control (CON), MI, and LIM + MI groups. Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein-protein interaction (PPI) networks were used to identify the biological functions and signaling pathways of differentially expressed mRNAs. Candidate genes were validated by RT-qPCR.

Results: Compared to the CON group, MI caused significant changes in the expression of 26 microRNAs and 1979 mRNAs. The bioinformatics analysis showed that inflammation, apoptosis, and oxidation were enriched in GO terms, while RAP1, PI3K/AKT, RAS, and cGMP-PKG were enriched in KEGG pathways. In addition, compared to the MI group, LIM induced significant changes in the expression of 4 microRNAs and 173 mRNAs. The differentially expressed mRNAs were related to collagen biosynthesis, the immune response, extrinsic apoptosis, and tight junctions. One microRNA (rno-miR-10a-5p) and 2 mRNAs (IGLON5 and LMX1A) were differentially expressed among the CON, MI, and LIM + MI groups.

Conclusions: Our results suggest that the rno-miR-10a-5p-IGLON5/LMX1A axis may be a candidate pathway and promising target through which LIM alleviates MI-induced cardiac dysfunction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2021.112088DOI Listing
October 2021

Ultra-sparse metamaterials absorber for broadband low-frequency sound with free ventilation.

J Acoust Soc Am 2021 Aug;150(2):1044

Key Laboratory of Modern Acoustics, Department of Physics and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

An absorptive device for broadband low-frequency sound with ventilation is essential but challenging in acoustic engineering, which is subjected to the narrow-band limitation and difficulty of balancing high-efficiency absorption and excellent ventilation. Here, we have theoretically and experimentally demonstrated an ultra-sparse (with filling ratio of 53.7%) broadband metamaterial absorber which can efficiently absorb (absorptance  >90%) sound energy ranging from 307 to 341 Hz, while enabling air to flow freely. The broadband absorber is constructed by parallel coupling four ventilated metamaterials absorbers (VMAs) showing different operating frequencies. Each VMA is composed of three folded Fabry-Pérot resonators as paste components, which are patched subsequently to the walls of a waveguide and correspondingly act as dark, middle, and bright modes following the coupled mode theory. In the VMA, the dark mode is highly over-damped to absorb sound energy, while the bright mode is highly under-damped to be an effective acoustic soft boundary, and the middle mode in-between should be slightly over-damped to strengthen the absorptions. Further investigation demonstrates that broadband high-efficiency absorption is robust against oblique incident angles. The proposed VMA provides a clear scheme for efficiently absorbing low-frequency sound while allowing free air flow simultaneously, which may prompt versatile applications in noise control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1121/10.0005850DOI Listing
August 2021

Aggregation-induced emission luminogens for highly effective microwave dynamic therapy.

Bioact Mater 2022 Jan 3;7:112-125. Epub 2021 Jun 3.

Department of Physics, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75080, USA.

Aggregation-induced emission luminogens (AIEgens) exhibit efficient cytotoxic reactive oxygen species (ROS) generation capability and unique light-up features in the aggregated state, which have been well explored in image-guided photodynamic therapy (PDT). However, the limited penetration depth of light in tissue severely hinders AIEgens as a candidate for primary or adjunctive therapy for clinical applications. Coincidentally, microwaves (MWs) show a distinct advantage for deeper penetration depth in tissues than light. Herein, for the first time, we report AIEgen-mediated microwave dynamic therapy (MWDT) for cancer treatment. We found that two AIEgens ( and ) served as a new type of microwave (MW) sensitizers to produce ROS, including singlet oxygen (O), resulting in efficient destructions of cancer cells. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and live/dead assays reveal that the two AIEgens when activated by MW irradiation can effectively kill cancer cells with average IC-50 values of 2.73 and 3.22 μM, respectively. Overall, the ability of the two AIEgens to be activated by MW not only overcomes the limitations of conventional PDT, but also helps to improve existing MW ablation therapy by reducing the MW dose required to achieve the same therapeutic outcome, thus reducing the occurrence of side-effects of MW radiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioactmat.2021.05.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379457PMC
January 2022

An integrated pore size distribution measurement method of small angle neutron scattering and mercury intrusion capillary pressure.

Sci Rep 2021 Aug 31;11(1):17458. Epub 2021 Aug 31.

Research Institute of Petroleum Exploration & Development, PetroChina Company Limited, Beijing, 100083, China.

Small-angle neutron scattering and high-pressure mercury intrusion capillary pressure testing are integrated to analyze the pore size distribution of the broad sense shale oil reservoir samples of the Permian Lucaogou Formation in the Jimsar Sag, Junggar Basin, China. The results show that, compared with the measurement method integrating gas adsorption and mercury intrusion, combination of small-angle neutron scattering and mercury intrusion can more accurately characterize full-scale pore size distribution. The full-scale pore size distribution curve of the rock samples in the study area includes two types: the declining type and submicron pore-dominated type. The declining type is mainly found with silty mudstone and dolomitic mudstone, and most of its pores are smaller than 80 nm. Silt-fine sandstones and dolarenite are mostly of the submicron pores-dominated type, with most pores smaller than 500 nm. They also present large specific pore volumes and average pore diameters of macropores and are the favorable lithogenous facies for development of high-quality reservoirs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-97027-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408136PMC
August 2021

Circular RNA circRNF13 inhibits proliferation and metastasis of nasopharyngeal carcinoma via SUMO2.

Mol Cancer 2021 08 31;20(1):112. Epub 2021 Aug 31.

NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410078, Hunan, China.

Background: Circular RNAs (circRNAs) are widely expressed in human cells and are closely associated with cancer development. However, they have rarely been investigated in the context of nasopharyngeal carcinoma (NPC).

Methods: We screened a new circRNA, circRNF13, in NPC cells using next-generation sequencing of mRNA. Reverse transcription polymerase chain reaction and RNA fluorescence in situ hybridization were used to detect circRNF13 expression in 12 non-tumor nasopharyngeal epithelial (NPE) tissues and 36 NPC samples. Cell proliferation was detected using MTT and flow cytometry assays, and colony formation capability was detected using colony formation assays. Cell migration and invasion were analyzed using wound-healing and Transwell assays, respectively. Cell glycolysis was analyzed using the Seahorse glycolytic stress test. Glucose transporter type 1 (GLUT1) ubiquitination and SUMOylation modifications were analyzed using co-immunoprecipitation and western blotting. CircRNF13 and Small Ubiquitin-like Modifier 2 (SUMO2) interactions were analyzed using RNA pull-down and luciferase reporter assays. Finally, to test whether circRNF13 inhibited NPC proliferation and metastasis in vivo, we used a xenograft nude mouse model generated by means of subcutaneous or tail vein injection.

Results: We found that circRNF13 was stably expressed at low levels in NPC clinical tissues and NPC cells. In vitro and in vivo experiments showed that circRNF13 inhibited NPC proliferation and metastasis. Moreover, circRNF13 activated the SUMO2 protein by binding to the 3'- Untranslated Region (3'-UTR) of the SUMO2 gene and prolonging the half-life of SUMO2 mRNA. Upregulation of SUMO2 promotes GLUT1 degradation through SUMOylation and ubiquitination of GLUT1, which regulates the AMPK-mTOR pathway by inhibiting glycolysis, ultimately resulting in the proliferation and metastasis of NPC.

Conclusions: Our results revealed that a novel circRNF13 plays an important role in the development of NPC through the circRNF13-SUMO2-GLUT1 axis. This study implies that circRNF13 mediates glycolysis in NPC by binding to SUMO2 and provides an important theoretical basis for further elucidating the pathogenesis of NPC and targeted therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12943-021-01409-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406723PMC
August 2021

Increased ranking change in wheat breeding under climate change.

Nat Plants 2021 Sep 30;7(9):1207-1212. Epub 2021 Aug 30.

Global Wheat Program, International Maize and Wheat Improvement Center, Texcoco, Mexico.

The International Maize and Wheat Improvement Center develops and annually distributes elite wheat lines to public and private breeders worldwide. Trials have been created in multiple sites over many years to assess the lines' performance for use in breeding and release as varieties, and to provide iterative feedback on refining breeding strategies. The collaborator test sites are experiencing climate change, with new implications for how wheat genotypes are bred and selected. Using a standard quantitative genetic model to analyse four International Maize and Wheat Improvement Center global spring wheat trial datasets, we examine how genotype-environment interactions have changed over recent decades. Notably, crossover interactions-a critical indicator of changes in the ranking of cultivar performance in different environments-have increased over time. Climatic factors explained over 70% of the year-to-year variability in crossover interactions for yield. Yield responses of all lines in trial environments from 1980 to 2018 revealed that climate change has increased the ranking change in breeding targeted to favourable environments by ~15%, while it has maintained or reduced the ranking change in breeding targeted to heat and drought stress by up to 13%. Genetic improvement has generally increased crossover interactions, particularly for wheat targeted to high-yielding environments. However, the latest wheat germplasm developed under heat stress was better adapted and more stable, partly offsetting the increase in ranking changes under the warmer climate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41477-021-00988-wDOI Listing
September 2021

Genetic Analysis and Literature Review of Variants in Parkinson's Disease.

Front Aging Neurosci 2021 12;13:648151. Epub 2021 Aug 12.

Center for Experimental Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.

Parkinson's disease (PD) is the fastest-growing neurodegenerative disorder. Aging, environmental factors, and genetics are considered as risk factors. The alpha-synuclein gene (), the first pathogenic gene identified in a familial form of PD, was indisputably involved as a heritable component for familial and sporadic PD. In this study, whole-exome sequencing and Sanger sequencing were performed to evaluate the association between the gene variants and PD. The genetic data of 438 clinically diagnosed patients with PD and 543 matched control populations of the Han Chinese were analyzed. The literature review of variants for 231 cases reported in 89 articles was extracted from the PubMed and the Movement Disorder Society Genetic mutation database. No potentially causative variant(s) in the gene, excepting two single-nucleotide nonsynonymous variants c.158C>T (p.A53V, rs542171324) and c.349C>T (p.P117S, rs145138372), were detected. There was no statistically significant difference in the genotypic or allelic frequencies for either variant between the PD group and the control group (all > 0.05). No copy number variants of the gene were detected. The results of this study suggest that the variants in the exons of the gene may have less or no role in the development of PD in the Han Chinese populations. The literature review suggests that psychiatric signs and cognitive decline/dementia were more common among patients with duplication or triplication (psychiatric signs: χ = 7.892, = 0.005; cognitive decline/dementia: χ = 8.991, = 0.003).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnagi.2021.648151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397385PMC
August 2021

The lncRNA-miRNA-mRNA ceRNA network in mural granulosa cells of patients with polycystic ovary syndrome: an analysis of Gene Expression Omnibus data.

Ann Transl Med 2021 Jul;9(14):1156

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine abnormalities in women of reproductive age. In this study, we set out to construct a molecular long non-coding RNA (lncRNA)-microRNA (miRNA)-messenger RNA (mRNA) network according to the competitive endogenous RNA (ceRNA) theory and obtain insights into the related biological characteristics and pathways.

Methods: We downloaded two gene expression profile datasets of mural granulosa cells (MGCs) of women with PCOS and healthy women without PCOS (GSE84376 and GSE106724) from Gene Expression Omnibus (GEO) DataSets. Using GEO2R, we identified the mRNAs and non-coding RNAs with differential expression. The DIANA-microT-CDS algorithm was applied to predict the genes targeted by the differentially expressed miRNAs. The lncRNA-miRNA interactions were predicted using DIANA-LncBase v2. Then, we constructed the lncRNA-miRNA-mRNA network. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was employed to identify the functions and enriched pathways of the genes. Subsequently, STRING was used to construct the protein-protein interaction (PPI) network. cytoHubba in Cytoscape was used to rank the hub genes, and finally, PPI modules were screened with Cytoscape MCODE.

Results: There were 462 mRNAs, 2,464 lncRNAs, and 55 miRNAs which showed differential expression between the MGCs of patients with PCOS and those of healthy controls. Based on the PPI analysis, differentially expressed genes (DEGs) were significantly enriched in retinol metabolism, drug metabolism-cytochrome P450, malaria, the Hippo signaling pathway, and glycine, serine, and threonine metabolism. The ceRNA network contained 71 lncRNA nodes, 14 miRNA nodes, and 69 mRNA nodes, as well as 167 edges. We identified some novel genes and non-coding RNAs that might be involved in PCOS, including , , , , , , , , , , and .

Conclusions: This study identified a novel lncRNA-miRNA-mRNA network based on the ceRNA mechanism in PCOS. Some novel genes and non-coding RNAs that may be involved in the occurrence and development of PCOS were excavated, including , , , , , , , , , , and . However, our findings need to be validated by and experiments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-2696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350636PMC
July 2021

2D materials-based homogeneous transistor-memory architecture for neuromorphic hardware.

Science 2021 09 19;373(6561):1353-1358. Epub 2021 Aug 19.

Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

[Figure: see text].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.abg3161DOI Listing
September 2021

RARγ activation sensitizes human myeloma cells to carfilzomib treatment through OAS-RNase L innate immune pathway.

Blood 2021 Aug 19. Epub 2021 Aug 19.

Research Institute of Houston Methodist Hospital, Houston, Texas, United States.

Proteasome inhibitors (PIs) such as bortezomib (Btz) and carfilzomib (Cfz) are highly efficacious for patients with multiple myeloma (MM). However, relapses are frequent and acquired resistance to PI treatment emerges in most patients. Here we performed a high-throughput screen of 1855 FDA-approved drugs and identified all-trans retinoic acid (ATRA), which alone has no antimyeloma effect, as a potent drug that enhanced MM sensitivity to Cfz-induced cytotoxicity and re-sensitized Cfz-resistant MM cells to Cfz in vitro. ATRA activated RARγ and IFN-β response pathway, leading to upregulated expression of IRF1. IRF1 in turn initiated the transcription of OAS1, which synthesized 2-5A upon binding to dsRNA induced by Cfz and resulted in cellular RNA degradation by RNase L and cell death. Similar to ATRA, BMS961, a selective RARγ agonist, could also (re)sensitize MM cells to Cfz in vitro, and both ATRA and BMS961 significantly enhanced the therapeutic effects of Cfz in established MM in vivo. In support of these findings, analyses of large patient's gene-profiling datasets showed a strong and positive correlation between RARγ and OAS1 expression and patient's response to PI treatment. Thus, this study highlights the potential for RARγ agonists to sensitize and overcome MM resistance to Cfz treatment in patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1182/blood.2020009856DOI Listing
August 2021

Integrated Analysis of a Competing Endogenous RNA Network Reveals a Prognostic lncRNA Signature in Bladder Cancer.

Front Oncol 2021 2;11:684242. Epub 2021 Aug 2.

Department of Urology, The Second Xiangya Hospital, Central South University, Changsha, China.

Long non-coding RNAs (lncRNAs) act as competing endogenous RNAs (ceRNAs) to regulate mRNA expression through sponging microRNA in tumorigenesis and progression. However, following the discovery of new RNA interaction, the differentially expressed RNAs and ceRNA regulatory network are required to update. Our study comprehensively analyzed the differentially expressed RNA and corresponding ceRNA network and thus constructed a potentially predictive tool for prognosis. "DESeq2" was used to perform differential expression analysis. Two hundred and six differentially expressed (DE) lncRNAs, 222 DE miRNAs, and 2,463 DE mRNAs were found in this study. The lncRNA-mRNA interactions in the miRcode database and the miRNA-mRNA interactions in the starBase, miRcode, and mirTarBase databases were searched, and a competing endogenous RNA (ceRNA) network with 186 nodes and 836 interactions was subsequently constructed. Aberrant expression patterns of lncRNA NR2F1-AS1 and lncRNA AC010168.2 were evaluated in two datasets (GSE89006, GSE31684), and real-time polymerase chain reaction was also performed to validate the expression pattern. Furthermore, we found that these two lncRNAs were independent prognostic biomarkers to generate a prognostic lncRNA signature by univariate and multivariate Cox analyses. According to the lncRNA signature, patients in the high-risk group were associated with a poor prognosis and validated by an external dataset. A novel genomic-clinicopathologic nomogram to improve prognosis prediction of bladder cancer was further plotted and calibrated. Our study deepens the understanding of the regulatory ceRNA network and provides an easy-to-do genomic-clinicopathological nomogram to predict the prognosis in patients with bladder cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.684242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366562PMC
August 2021

Femoral neck system versus inverted cannulated cancellous screw for the treatment of femoral neck fractures in adults: a preliminary comparative study.

J Orthop Surg Res 2021 Aug 16;16(1):504. Epub 2021 Aug 16.

Department of Orthopaedics, West China Hospital, West China School of Medicine, Sichuan University, No. 37, Wuhou Guoxue Road, 610041, Chengdu, Sichuan, People's Republic of China.

Background: The femoral neck system (FNS) may be a valid alternative for treating femoral neck fractures, but few studies have compared the outcomes between FNS and cannulated cancellous screws. This study aimed to compare the clinical efficacy and complications of FNS with those of inverted cannulated cancellous screws (ICCS) for the treatment of femoral neck fractures in adults.

Methods: This retrospective study evaluated patients with femoral neck fractures who underwent FNS or ICCS internal fixation between June 2019 and January 2020. The operative time, intraoperative blood loss, number of fluoroscopies, length of surgical incision, length of hospital stay, time to fracture healing, and other indicators were compared between the two groups. The incidence of complications such as nonunion, avascular necrosis of the femoral head, loosening of the internal fixation, and shortening of the femoral neck during follow-up was also assessed in both groups.

Results: Among the 92 patients included, 47 and 45 patients were categorized into the FNS and ICCS groups, respectively. Follow-up ranged from 14 to 24 months. There were no significant between-group differences in sex, age, side of the injured limb, and type of fracture and in operative time, intraoperative blood loss, incision length, and length of hospital stay (all P > 0.05). The incidence of complications such as fracture nonunion, avascular necrosis of the femoral head, and screw loosening was also comparable between the two groups. However, the number of fluoroscopies was significantly lower in the FNS group than in the ICCS group (P < 0.05). The fracture healing time was also significantly lower in the FNS group than in the ICCS group (P < 0.05). Furthermore, the femoral neck shortening and Harris hip score at the last follow-up were significantly better in the FNS group than in the ICCS group (P < 0.05).

Conclusions: FNS for femoral neck fractures improves hip functional recovery and reduces the femoral neck shortening rate and fluoroscopy exposure postoperatively. Thus, FNS can be an alternative to ICCS for the treatment of femoral neck fractures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02659-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365927PMC
August 2021

Evaluation of efficiency and safety of muscular region's acupuncture treatments for knee osteoarthritis: A protocol for systematic review and network meta-analysis.

Medicine (Baltimore) 2021 Aug;100(31):e26810

Nanchang Hongdu Hospital of Traditional Chinese Medicine, Nanchang, Jiangxi Province, China.

Background: As a chronic degenerative disease, knee osteoarthritis (KOA) is mainly characterized by loose ligaments around the knee joint, degeneration of cartilage in the knee joint, and atrophy of surrounding muscles. According to related investigations, the incidence of knee osteoarthritis in China is 8.1%, of which 10.3% are women and 5.7% are men. Therefore, in order to improve the therapeutic effect of KOA, we must constantly explore new ways to treat the disease. The purpose of this study is to evaluate the effectiveness and safety of acupuncture with needle knife, blade needle, long-round needle, fire needle, micro-needle knife with conventional acupuncture intervention in KOA.

Methods: Computer search of PubMed, Cochrane Library, Web of Science, CNKI, Wangfang, and VIP database, search for randomized controlled trials of muscular region's acupuncture therapy on KOA, the search time limit is to build the database until July 9, 2021. To ensure the comprehensiveness of the search, relevant references and conference literature are also included. After 2 researchers independently screened the literature, extracted data, and evaluated the risk of bias in the included studies, the Stata 14.0 software was used for data analysis.

Results: The effectiveness and safety of muscular region's acupuncture in the treatment of patients with KOA will be systematically evaluated.

Conclusion: The results of this study will provide strong evidence to determine whether muscular region's acupuncture is effective in the treatment of KOA.

Registration Number: INPLASY202170031 (https://inplasy.com/inplasy-2021-7-0031/).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341343PMC
August 2021

Oncostatin M Receptor-targeted antibodies suppress STAT3 signaling and inhibit ovarian cancer growth.

Cancer Res 2021 Aug 11. Epub 2021 Aug 11.

Department of Obstetrics any Gynecology, Medical College of Wisconsin

While patients with advanced ovarian cancer may respond initially to treatment, disease relapse is common and nearly 50% of patients do not survive beyond five years, indicating an urgent need for improved therapies. To identify new therapeutic targets, we performed single cell and nuclear RNA-seq dataset analyses on 17 human ovarian cancer specimens, revealing the oncostatin M receptor (OSMR) as highly expressed in ovarian cancer cells. Conversely, oncostatin M (OSM), the ligand of OSMR, was highly expressed by tumor-associated macrophages and promoted proliferation and metastasis in cancer cells. Ovarian cancer cell lines and additional patient samples also exhibited elevated levels of OSMR when compared to other cell types in the tumor microenvironment or to normal ovarian tissue samples. OSMR was found to be important for ovarian cancer cell proliferation and migration. Binding of OSM to OSMR caused OSMR-IL6ST dimerization, which is required to produce oncogenic signaling cues for prolonged STAT3 activation. Human monoclonal antibody clones B14 and B21 directed to the extracellular domain of OSMR abrogated OSM-induced OSMR-IL6ST heterodimerization, promoted the internalization and degradation of OSMR, and effectively blocked OSMR-mediated signaling in vitro. Importantly, these antibody clones inhibited the growth of ovarian cancer cells in vitro and in vivo by suppressing oncogenic signaling through OSMR and STAT3 activation. Collectively, this study provides a proof of principle that anti-OSMR antibody can mediate disruption of OSM-induced OSMR-IL6ST dimerization and oncogenic signaling, thus documenting the pre-clinical therapeutic efficacy of human OSMR antagonist antibodies for immunotherapy in ovarian cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/0008-5472.CAN-21-0483DOI Listing
August 2021

Pollution-driven morphological plasticity in a running water ecosystem.

Environ Sci Pollut Res Int 2021 Aug 10. Epub 2021 Aug 10.

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing, 100085, China.

Various types of pollutants derived from rapid industrialization and urbanization have largely threaten biodiversity and functioning of freshwater ecosystems globally. Morphological plasticity, especially body size-associated traits, is considered a functional response to water pollution in species, as such changes are often directly related to functioning of freshwater ecosystems through dynamics of food webs. However, detailed dynamics of pollution impacts on morphological plasticity remain largely unknown, particularly in the wild. Here, we used the model planktonic rotifer Brachionus calyciflorus to assess morphological response to chemical pollution in a river reach disturbed by sewage discharges. Multiple analyses showed dynamic morphological response to water pollution in wild B. calyciflorus populations. The distance between anterior lateral spines, lorica length, and egg short diameter were the most sensitive morphological indicators to water pollution, while spine length was stable in varied pollution conditions. Interestingly, body size and egg size were increased with accentuated water pollution, suggesting that wild populations maintain fitness by increasing feeding efficiency and reducing vulnerability to predation and ensure survival by producing large newborns in polluted environments. Total ammonia nitrogen was the leading nitrogen pollutant affecting body size, while total phosphorus and elements of Mn and As were the key factors relating to egg size. The results obtained here provide new sights into biological consequences of environmental pollution in the wild, thus advancing our understanding of pollution impacts on structure and functioning of freshwater ecosystems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15802-5DOI Listing
August 2021
-->