Publications by authors named "Wei Xia"

1,300 Publications

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An automatic detection method of cerebral aneurysms in time-of-flight magnetic resonance angiography images based on attention 3D U-Net.

Comput Methods Programs Biomed 2022 Jul 1;225:106998. Epub 2022 Jul 1.

Department of Radiology, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China.

Background: Subarachnoid hemorrhage caused by ruptured cerebral aneurysm often leads to fatal consequences. However, if the aneurysm can be found and treated during asymptomatic periods, the probability of rupture can be greatly reduced. At present, time-of-flight magnetic resonance angiography is one of the most commonly used non-invasive screening techniques for cerebral aneurysm, and the application of deep learning technology in aneurysm detection can effectively improve the screening effect of aneurysm. Existing studies have found that three-dimensional features play an important role in aneurysm detection, but they require a large amount of training data and have problems such as a high number of FPs per case.

Methods: This paper proposed a novel method for aneurysm detection. First, a fully automatic cerebral artery segmentation algorithm without training data was used to extract the volume of interest, and then the 3D U-Net was improved by the 3D SENet module to establish an aneurysm detection model. Eventually a set of fully automated, end-to-end aneurysm detection methods have been formed.

Results: A total of 231 magnetic resonance angiography image data were used in this study, among which 132 were training sets, 34 were internal test sets and 65 were external test sets. The presented method obtained 97.89±0.88% sensitivity in the five-fold cross-validation and obtained 90.8% sensitivity with 2.47 FPs/case in the detection of the external test sets.

Conclusions: Compared with the results of our previous studies and other studies, the method in this paper achieves the best sensitivity while maintaining low number of FPs per case. This result proves the feasibility, superiority, and further improvement potential of the improved method combining 3D U-Net and channel attention in the task of aneurysm detection.
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http://dx.doi.org/10.1016/j.cmpb.2022.106998DOI Listing
July 2022

Oxidative degradation of UV-irradiated polyethylene by laccase-mediator system.

J Hazard Mater 2022 Aug 3;440:129709. Epub 2022 Aug 3.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address:

Polyethylene (PE) is one of the most widely used plastics. However, the chemical inertness, inefficient recycling, and random landfilling of PE waste have caused serious pollution to the natural environment. In this study, a series of laccase-mediator systems (LMS) were constructed by combination of two laccases from Botrytis aclada (BaLac) and Bacillus subtilis (BsLac) with three synthetic mediators (ABTS, HBT, and TEMPO) to oxidize LDPE films (UVPE) pretreated with high-temperature UV irradiation. Scanning electron microscopy showed aging phenomena such as etching, fragmentation, and cracking on the surface of the UVPE films after LMS incubation. The FTIR results showed that LMS-UVPE added new oxygen-containing functional groups such as -OH, -CO, and CC. High-temperature gel chromatography confirmed that the average reduction in weight-average molecular weight (Mw) was approximately 40% for the BaLac experimental group. GC-MS analysis showed the presence of oxygen-containing products, such as aldehydes, ketones, and alcohols, in the reaction mixture. To verify the oxidation process UVPE degradation by LMS, we inferred three possible pathways by combined analysis of the oxidation products of LMS on UVPE and model substrates oleic acid and squalene.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129709DOI Listing
August 2022

Effectiveness and Cost-Effectiveness of Inactivated Vaccine to Address COVID-19 Pandemic in China: Evidence From Randomized Control Trials and Real-World Studies.

Front Public Health 2022 19;10:917732. Epub 2022 Jul 19.

School of Public Health, Peking University, Beijing, China.

Objective: This study aimed to determine the efficacy, effectiveness, and cost-effectiveness of inactivated COVID-19 vaccines (CoronaVac and BBIBP-CorV) in China using existing international clinical trials and real-world evidence.

Methods: Through a search of PubMed, Embase, Web of Science, and CNKI, studies investigating the effectiveness of inactivated COVID-19 vaccines were identified, and a meta-analysis was undertaken to synthesize the vaccine efficacy and effectiveness data. Moreover, a decision-analytic model was developed to estimate the cost-effectiveness of inactivated vaccines for combating the COVID-19 pandemic in the Chinese context from a societal perspective. Results of the meta-analysis, along with cost data from official websites and works of literature were used to populate the model. Sensitivity analysis was performed to test the robustness of the model results.

Results: A total of 24 studies were included in the meta-analysis. In comparison to no immunization, the effectiveness of inactivated vaccine against COVID-19 infection, hospitalization, ICU admission and death were 65.18% (95% CI 62.62, 67.75), 79.10% (95% CI 71.69, 86.51), 90.46% (95% CI 89.42, 91.50), and 86.69% (95% CI 85.68, 87.70); and the efficacy against COVID-19 infection and hospitalization were 70.56% (95% CI 57.87, 83.24) and 100% (95% CI 61.72, 100). Inactivated vaccine vaccination prevented more infections, hospitalizations, ICU admissions, and deaths with lower total costs, thus was cost-saving from a societal perspective in China. Base-case analysis results were robust in the one-way sensitivity analysis, and the percentage of ICU admission or death and direct medical cost ranked the top influential factors in our models. In the probabilistic sensitivity analysis, vaccination had a 100% probability of being cost-effective.

Conclusion: Inactivated vaccine is effective in preventing COVID-19 infection, hospitalization, ICU admission and avoiding COVID-19 related death, and COVID-19 vaccination program is cost-saving from societal perspective in China.
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http://dx.doi.org/10.3389/fpubh.2022.917732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343737PMC
August 2022

Efficacy, safety and immunogenicity of hexavalent rotavirus vaccine in Chinese infants.

Virol Sin 2022 Aug 1. Epub 2022 Aug 1.

National Engineering Technology Research Center for Combined Vaccines, Wuhan Institute of Biological Product Co., Ltd., Wuhan, 430207, China.

A randomized, double-blind, placebo-controlled multicenter trial was conducted in healthy Chinese infants to assess the efficacy and safety of a hexavalent live human-bovine reassortant rotavirus vaccine (HRV) against rotavirus gastroenteritis (RVGE). A total of 6400 participants aged 6-12 weeks were enrolled and randomly assigned to either HRV (n = 3200) or placebo (n = 3200) group. All the subjects received three oral doses of vaccine four weeks apart. The vaccine efficacy (VE) against RVGE caused by rotavirus serotypes contained in HRV was evaluated from 14 days after three doses of administration up until the end of the second rotavirus season. VE against severe RVGE, VE against RVGE hospitalization caused by serotypes contained in HRV, and VE against RVGE, severe RVGE, and RVGE hospitalization caused by naturally infecting any serotype of rotavirus were also investigated. All adverse events (AEs) were collected for 30 days after each dose. Serious AEs (SAEs) and intussusception cases were collected during the entire study. Our data showed that VE against RVGE caused by serotypes contained in HRV was 69.21% (95%CI: 53.31-79.69). VE against severe RVGE and VE against RVGE hospitalization caused by serotypes contained in HRV were 91.36% (95%CI: 78.45-96.53) and 89.21% (95%CI: 64.51-96.72) respectively. VE against RVGE, severe RVGE, and RVGE hospitalization caused by naturally infecting any serotype of rotavirus were 62.88% (95%CI: 49.11-72.92), 85.51% (95%CI: 72.74-92.30) and 83.68% (95%CI: 61.34-93.11). Incidences of AEs from the first dose to one month post the third dose in HRV and placebo groups were comparable. There was no significant difference in incidences of SAEs in HRV and placebo groups. This study shows that this hexavalent reassortant rotavirus vaccine is an effective, well-tolerated, and safe vaccine for Chinese infants.
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http://dx.doi.org/10.1016/j.virs.2022.07.011DOI Listing
August 2022

Intracavity generation of glioma stem cell-specific CAR macrophages primes locoregional immunity for postoperative glioblastoma therapy.

Sci Transl Med 2022 Aug 3;14(656):eabn1128. Epub 2022 Aug 3.

Key Laboratory of Chemical Biology (Ministry of Education), Department of Pharmaceutics, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Cultural West Road, Jinan, Shandong Province 250012, China.

Glioblastoma multiforme (GBM) remains incurable despite aggressive implementation of multimodal treatments after surgical debulking. Almost all patients with GBM relapse within a narrow margin around the initial resected lesion due to postsurgery residual glioma stem cells (GSCs). Tracking and eradicating postsurgery residual GSCs is critical for preventing postoperative relapse of this devastating disease, yet effective strategies remain elusive. Here, we report a cavity-injectable nanoporter-hydrogel superstructure that creates GSC-specific chimeric antigen receptor (CAR) macrophages/microglia (MΦs) surrounding the cavity to prevent GBM relapse. Specifically, we demonstrate that the CAR gene-laden nanoporter in the hydrogel can introduce GSC-targeted CAR genes into MΦ nuclei after intracavity delivery to generate CAR-MΦs in mouse models of GBM. These CAR-MΦs were able to seek and engulf GSCs and clear residual GSCs by stimulating an adaptive antitumor immune response in the tumor microenvironment and prevented postoperative glioma relapse by inducing long-term antitumor immunity in mice. In an orthotopic patient-derived glioblastoma humanized mouse model, the combined treatment with nanoporter-hydrogel superstructure and CD47 antibody increased the frequency of positive immune responding cells and suppressed the negative immune regulating cells, conferring a robust tumoricidal immunity surrounding the postsurgical cavity and inhibiting postoperative glioblastoma relapse. Therefore, our work establishes a locoregional treatment strategy for priming cancer stem cell-specific tumoricidal immunity with broad application in patients suffering from recurrent malignancies.
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http://dx.doi.org/10.1126/scitranslmed.abn1128DOI Listing
August 2022

Solution structure of a thrombin binding aptamer complex with a non-planar platinum(ii) compound.

Chem Sci 2022 Jul 23;13(28):8371-8379. Epub 2022 Jun 23.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University Guangzhou 510275 China

Thrombin Binding Aptamer (TBA) is a monomolecular well-defined two G-tetrad antiparallel G-quadruplex DNA that inhibits the activity of human α-thrombin. In this report, we synthesized a quasi-cross-shaped platinum(ii) compound (L'LPt) with one cyclometalated and two carbene ligands. We found L'LPt has selective affinity to bind the TBA G-quadruplex. A fibrinogen clotting assay revealed that L'LPt can abrogate the inhibitory activity of TBA against thrombin. We solved the 1 : 1 L'LPt-TBA complex structure by NMR, which revealed a unique self-adaptive property of L'LPt upon binding to TBA. In the complex, a carbene ligand of L'LPt rotates to pair with the cyclometalated ligand to form a plane stacking over half of the TBA G-tetrad and covered by lateral TT loops. It is notable that the heavy atom Pt stays out of the G-tetrad. Meanwhile, the other carbene ligand remains relatively perpendicular and forms a hydrogen bond with a guanine to anchor the L'LPt position. This structure exhibits a quasi-cross-shaped Pt(ii) compound bound to the G-quadruplex with an unusual "wall-mounted" binding mode. Our structures provide insights into the specific recognition of antiparallel G-quadruplex DNA by a self-adaptive Pt(ii) compound and useful information for the design of selective G-quadruplex targeting non-planar molecules.
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http://dx.doi.org/10.1039/d2sc01196dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297526PMC
July 2022

Relationship between Yes-Associated Protein 1 and Prognosis of Digestive System Neoplasm: Quantitative Analysis and Bioinformatics Analysis Based on 4023 Patients.

Evid Based Complement Alternat Med 2022 19;2022:3798694. Epub 2022 Jul 19.

Department of Gastroenterology, Daping Hospital, Army Medical University, Chongqing 400042, China.

Yes-associated protein 1 (YAP1) is involved in the development of a variety of malignancies. However, the prognosis of malignant digestive tumors with YAP1 expression is still controversial. This study searched 31 articles with 36 data sets of 4023 patients to explore the role of YAP1 expression on the prognosis of digestive malignant tumors by searching the PubMed, Embase, Web of Science, Google Scholar, and Cochrane Library databases. Specifically, relevant cancer expression matrix data were downloaded from The Cancer Genome Atlas (TCGA) database. In this meta-analysis, quantitative analysis showed that the overexpression of YAP1 was not conducive to OS (1.62, 95% CI (1.38, 1.90), =0.001) and DFS (1.59, 95% CI (1.31, 1.93), =0.001) in patients with digestive malignant tumors. In addition, TCGA database analysis showed that YAP1 was overexpressed in gastric cancer, cholangiocarcinoma, and colorectal cancer. Survival analysis showed that the patients with high expression of YAP1 in pancreatic cancer have a poor OS (MST: 394 vs. 691 days, < 0.0001) and DFS (MST: 371 vs. 542 days, =0.026) prognosis. YAP1 may be a molecular marker that effectively predicts the survival of malignant digestive tumors, especially pancreatic cancer, and is a potential therapeutic target for malignant digestive tumors.
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http://dx.doi.org/10.1155/2022/3798694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325623PMC
July 2022

A review of the effects of estrogen and epithelial-mesenchymal transformation on intrauterine adhesion and endometriosis.

Transpl Immunol 2022 Jul 28:101679. Epub 2022 Jul 28.

Institute of Reproductive Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Uterus transplantation has become an option for women suffering from some form of infertility. Current review discusses key physiological functions of the endometrium requiring the transition of tissue cells between the mesenchyme and epithelial cell phenotype, a process known as epithelial-mesenchymal transition (EMT). Estrogen and EMT play a key role in the pathogenesis and treatment of intrauterine adhesion and endometriosis. There is also a close regulatory relationship between estrogen and EMT, and investigation of this relationship is of great significance for the treatment of endometrial disorders. The present review discusses the effects of estrogen on endometrial dysfunction, with a focus on the relationship between estrogen and EMT in endometrial disorders, taking into consideration the mechanisms by which receptors that regulate their functions and proteins that regulate their local biological functions interact with the factors involved in EMT. In addition, the review summarizes emerging drugs targeting receptors or proteins and provides information on the direction of new therapies for endometrial disorders.
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http://dx.doi.org/10.1016/j.trim.2022.101679DOI Listing
July 2022

Experimental study of impact mechanical and microstructural properties of modified carbon fiber reinforced concrete.

Sci Rep 2022 Jul 28;12(1):12928. Epub 2022 Jul 28.

Aviation Engineering School, Air Force Engineering University, Xi'an, 710038, China.

This paper investigated the preparation method and the dispersion behaviour of Modified Carbon Nanotube-fiber Reinforcements (MCNF), the change laws and the effect mechanisms of dynamic compressive strength of MCNF concretes. Electrophoresis method was used to prepare MCNF and its interfacial shear performance was tested by interfacial shear strength (IFSS) test. In addition, the dispersion behavior of MCNF in simulated concrete solution was verified by turbidity method. Split Hopkinson Pressure Bar (SHPB), Scanning Electron Microscope (SEM) and Mercury Intrusion Porosimetry (MIP) tests were carried on concrete samples with different volume fractions (0%, 0.1%, 0.2%, 0.3%, 0.4%) of MCNF. The results show that carbon nanotubes are easier to deposit to the negative electrode, and the higher the content of polycarboxylate superplasticizer, the more obvious the dispersity of MCNF in alkaline environment. The dynamic compressive strength of MCNF concrete was 14.0-35.5% higher than that of untreated concrete, and reached the maximum when the MCNF content was about 0.3%. The MCNF was wrapped in concrete matrix and promoted hydration reaction of interface between cement and MCNF from microscopic observation. The addition of MCNF could increase the porosity. The volume percentage of ≥ 100 nm pore decreased first and then increased. Reasons for the improvement strength of MCNF concrete is that the bridging effect is stronger with the increase of MCNF content (≤ 0.3%) and limited when the MCNF content is equal to 0.4%. MCNF concrete could be used in actual engineering with high requirements for dynamic load.
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http://dx.doi.org/10.1038/s41598-022-17092-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334606PMC
July 2022

Efficacy and safety of focused low-intensity pulsed ultrasound versus pulsed shortwave diathermy on knee osteoarthritis: a randomized comparative trial.

Sci Rep 2022 Jul 27;12(1):12792. Epub 2022 Jul 27.

State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China.

The aim of this study was to compare the efficacy and safety of focused low-intensity pulsed ultrasound (FLIPUS) with pulsed shortwave diathermy (PSWD) in subjects with painful knee osteoarthritis (OA). In a prospective randomized trial, 114 knee OA patients were randomly allocated to receive FLIPUS or PSWD therapy. The primary outcome was the change from baseline in the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total scores. Secondary outcomes included the numerical rating scale (NRS) for pain assessment, time up and go (TUG) test, active joint range of motion (ROM) test, and Global Rating of Change (GRC) scale. Data were collected at baseline, 12 days, 12 weeks and 24 weeks. Patients receiving FLIPUS therapy experienced significantly greater improvements in the WOMAC total scores than patients receiving PSWD therapy at 12 days (mean difference, - 10.50; 95% CI - 13.54 to - 7.45; P = 0.000). The results of the NRS, TUG test, ROM test and GRC scale showed that participants treated with FLIPUS reported less pain and better physical function and health status than those treated with PSWD at 12 days (P = 0.011, P = 0.005, P = 0.025, P = 0.011, respectively). Furthermore, patients in the FLIPUS group showed significant improvements in the WOMAC total scores and NRS scores at 12 weeks (mean difference, - 7.57; 95% CI - 10.87 to - 4.26; P = 0.000 and - 1.79; 95% CI - 2.11 to - 1.47, respectively) and 24 weeks (mean difference, - 6.96; 95% CI - 10.22 to - 3.71; P = 0.000 and - 1.37; 95% CI - 1.64 to - 0.96; P = 0.000, respectively) of follow-up. There were no adverse events during or after the interventions in either group. This study concluded that both FLIPUS and pulsed SWD are safe modalities, and FLIPUS was more effective than PSWD in alleviating pain and in improving dysfunction and health status among subjects with knee OA in the short term.Trial registration: Chinese Clinical Trial Registry, ChiCTR2000032735. Registered 08/05/2020, http://www.chictr.org.cn/showproj.aspx?proj=53413 .
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http://dx.doi.org/10.1038/s41598-022-17291-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329394PMC
July 2022

Preoperative CECT-based Radiomic Signature for Predicting the Response of Transarterial Chemoembolization (TACE) Therapy in Hepatocellular Carcinoma.

Cardiovasc Intervent Radiol 2022 Jul 27. Epub 2022 Jul 27.

Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou, 215006, Jiangsu, China.

Purpose: To evaluate the efficiency of radiomics signatures in predicting the response of transarterial chemoembolization (TACE) therapy based on preoperative contrast-enhanced computed tomography (CECT).

Materials: This study consisted of 111 patients with intermediate-stage hepatocellular carcinoma who underwent CECT at both the arterial phase (AP) and venous phase (VP) before and after TACE. According to mRECIST 1.1, patients were divided into an objective-response group (n = 38) and a non-response group (n = 73). Among them, 79 patients were assigned as the training dataset, and the remaining 32 cases were assigned as the test dataset.

Methods: Radiomics features were extracted from CECT images. Two feature ranking methods and three classifiers were used to find the best single-phase radiomics signatures for both AP and VP on the training set. Meanwhile, multi-phase radiomics signatures were built upon integration of images from two CECT phases by decision-level fusion and feature-level fusion. Finally, multivariable logistic regression was used to develop a nomogram by combining radiomics signatures and clinic-radiologic characteristics. The prediction performance was evaluated by AUC on the test dataset.

Results: The multi-phase radiomics signature (AUC = 0.883) performed better in predicting TACE therapy response compared to the best single-phase radiomics signature (AUC = 0.861). The nomogram (AUC = 0.913) showed better performance than any radiomics signatures.

Conclusion: The radiomics signatures and nomogram were developed and validated for predicting responses to TACE therapy, and the radiomics model may play a positive role in identifying patients who may benefit from TACE therapy in clinical practice.
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http://dx.doi.org/10.1007/s00270-022-03221-zDOI Listing
July 2022

Purification and Identification of Flavonoid Molecules from Waste Extracts and Evaluation of Antioxidant, Antiproliferative and Antimicrobial Activities.

Molecules 2022 Jul 8;27(14). Epub 2022 Jul 8.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

is widely used in the essential oil industry and generates large amounts of waste annually. The purpose of this research is the recycling of bioactive flavonoids from rose waste biomass to develop high-value products. Resin screening and adsorption/desorption dynamic analysis showed that HP20 resin was suitable to purify the flavonoids from waste extracts. Under the optimal enrichment process, the product had a 10.7-fold higher purity of flavonoids with a satisfactory recovery of 82.02%. In total, 14 flavonoids were identified in the sample after purification by UHPLC-QTOF-MS. Moreover, the DPPH and ABTS assays revealed that the flavonoids-purified extracts exhibited higher antioxidant activities than the crude extracts. Meanwhile, the purified extracts presented stronger antiproliferative activity against HepG2, Caco-2, MCF-7 and A549 cell lines. The bacteriostatic effects of the purified extracts against four bacteria (, , , )) and yeast ()) were stronger compared with the crude extracts. It was concluded that flavonoids-enriched extracts from waste had the potential to be applied in functional food and pharmaceutical industries.
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http://dx.doi.org/10.3390/molecules27144379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323010PMC
July 2022

Rare Carbon-Bridged Citrinin Dimers from the Starfish-Derived Symbiotic Fungus sp. GGF16-1-2.

Mar Drugs 2022 Jul 6;20(7). Epub 2022 Jul 6.

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

Four novel, rare carbon-bridged citrinin dimers, namely dicitrinones G-J (-), and five known analogs (-) were isolated from the starfish-derived fungus sp. GGF 16-1-2. Their structures were elucidated by extensive spectroscopic analysis and quantum chemical calculations. Compounds - exhibited strong antifungal activities against with LD values from 0.61 μg/mL to 16.14 μg/mL. Meanwhile, all compounds were evaluated for their cytotoxic activities against human pancreatic cancer BXPC-3 and PANC-1 cell lines; as a result, compound showed more significant cytotoxicities than the positive control against both cell lines. In addition, based on the analyses of the protein-protein interaction (PPI) network and Western blot, could induce apoptosis by activating caspase 3 proteins (CASP3).
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http://dx.doi.org/10.3390/md20070443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317178PMC
July 2022

Unusual Ether Lipids and Branched Chain Fatty Acids in Sea Cucumber () Viscera and Their Seasonal Variation.

Mar Drugs 2022 Jun 29;20(7). Epub 2022 Jun 29.

Agriculture and Agri-Food Canada, Kentville, NS B4N 1J5, Canada.

The sea cucumber, , is harvested primarily for its muscular bands and body wall. Development of a nutraceutical product based on lipid recovered from its viscera would give commercial value to the entire organism; however, such development requires knowledge of the lipid and fatty acid (FA) profiles of the viscera. Here, we describe the lipid and FA composition of viscera recovered from harvested in coastal waters in the northwest Atlantic, taking into account variation due to harvest season. We found highest lipid content at ~29% in winter, with diacylglyceryl ethers (DAGE) comprising ~55% of the total lipid mass and triacylglycerols (TAG), phospholipids (PL) and monoacylglycerol ethers (MAGE) at 5-25% each. The branched chain FA, 12-methyltetradecanoic acid (12-MTA), represented 42% of total FA mass in DAGE. In summer, lipid content was lower at 24% and TAG was the dominate lipid, with proportions more than double that found in winter (45% vs. 20%); DAGE in summer dropped to ~30% of total lipids. In TAG, 12-MTA was much lower than found in DAGE in winter, at only 10% but eicosapentaenoic acid (EPA) content was ~20%, which brought the total EPA% to 28% of total FA-the highest among all three seasons. There was little effect of season on MAGE or PL proportions. These data can help harvesters maximize catch efforts in terms of lipid yield and profile.
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http://dx.doi.org/10.3390/md20070435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318488PMC
June 2022

Preparation of trimetallic electrocatalysts by one-step co-electrodeposition and efficient CO reduction to ethylene.

Chem Sci 2022 Jun 10;13(25):7509-7515. Epub 2022 Jun 10.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University Shanghai 200062 China

Use of multi-metallic catalysts to enhance reactions is an interesting research area, which has attracted much attention. In this work, we carried out the first work to prepare trimetallic electrocatalysts by a one-step co-electrodeposition process. A series of Cu-X-Y (X and Y denote different metals) catalysts were fabricated using this method. It was found that CuLaCs (the content ratio of Cu, La, and Cs in the electrolyte is 10 : 1 : 1 in the deposition process), which had an elemental composition of CuLaCs in the catalyst, formed a composite structure on three dimensional (3D) carbon paper (CP), which showed outstanding performance for CO electroreduction reaction (CORR) to produce ethylene (CH). The faradaic efficiency (FE) of CH could reach 56.9% with a current density of 37.4 mA cm in an H-type cell, and the partial current density of CH was among the highest ones up to date, including those over the catalysts consisting of Cu and noble metals. Moreover, the FE of C products (CH, ethanol, and propanol) over the CuLaCs catalyst in a flow cell reached 70.5% with a high current density of 486 mA cm. Experimental and theoretical studies suggested that the doping of La and Cs into Cu could efficiently enhance the reaction efficiency a combination of different effects, such as defects, change of electronic structure, and enhanced charge transfer rate. This work provides a simple method to prepare multi-metallic catalysts and demonstrates a successful example for highly efficient CORR using non-noble metals.
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http://dx.doi.org/10.1039/d1sc06964kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241956PMC
June 2022

Association of exposure to ambient particulate matter with maternal thyroid function in early pregnancy.

Environ Res 2022 Jul 21;214(Pt 2):113942. Epub 2022 Jul 21.

School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Background: It is known that maternal thyroid dysfunction during early pregnancy can cause adverse pregnancy complications and birth outcomes. This study was designed to examine the association between ambient particulate matter with aerodynamic diameters ≤2.5 μm (PM) and particulate matter with aerodynamic diameters ≤10 μm (PM) exposure and maternal thyroid function during early pregnancy.

Methods: This study was based on data from a birth cohort study of 921 pregnant women in China. We estimated associations between ambient PM and PM exposure during the first trimester of pregnancy (estimated with land-use regression models) and maternal thyroid hormone concentrations (free thyroxine (FT4), free tri-iodothyronine (FT3), and thyroid-stimulating hormone (TSH)) collected between weeks 10 and 17 of gestation using linear regression models adjusting for potential confounders. Ambient PM and PM concentrations were modeled per interquartile range (IQR) increment and as tertiles based on the distribution of the exposure levels.

Results: An IQR increment (68 μg/m) in PM exposure was associated with a significant decrease in maternal FT4 levels (β = -0.60, 95% CI: -1.07, -0.12); and a significant decrease in FT4/FT3 ratio (β = -0.13, 95% CI: -0.25, -0.02). Further analyses showed that, relative to the lowest tertile, women in both the middle and highest tertiles of PM had significantly lower concentrations of maternal FT4 and FT4/FT3 ratio. No significant associations were found between PM and FT3 or TSH levels. PM exposure was not significantly associated with maternal thyroid function.

Conclusions: Our study suggested that higher ambient PM, not PM exposed during the first trimester of pregnancy were associated with a significant decrease in maternal serum FT4 concentrations and FT4/FT3 ratio. Studies in populations with different exposure levels are needed to replicate our study results.
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http://dx.doi.org/10.1016/j.envres.2022.113942DOI Listing
July 2022

Photocatalytic Cleavage of C(sp )-N Bond in Trialkylamines to Dialkylamines and Olefins.

ChemSusChem 2022 Jul 12:e202201119. Epub 2022 Jul 12.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200062, P. R. China.

Development of a new and green strategy for C(sp )-N bond cleavage is very interesting. Herein, photocatalytic cleavage of the C(sp )-N bond of trialkylamines was achieved, with concurrent formation of dialkylamines and olefins. It was found that a rationally designed 2D-Bi WO @1D-LaPO heterostructure was very efficient for the reaction due to its high light collection efficiency and unique catalytic properties. The strategy could be used for different trialkylamines, including triethylamine, tri-n-propylamine, and ethyl-di-isopropylamine. The mechanistic investigation indicated that the catalyst with heterostructure was not only favorable for charge carrier separation but also rendered excited electrons with high reduction capacity. This work opens a way for C(sp )-N bond cleavage of trialkylamines.
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http://dx.doi.org/10.1002/cssc.202201119DOI Listing
July 2022

Effects of surface treatment and shade on the color, translucency, and surface roughness of high-translucency self-glazed zirconia materials.

J Prosthet Dent 2022 Jul 8. Epub 2022 Jul 8.

Associate Professor, Department of General Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China. Electronic address:

Statement Of Problem: The impact of different surface treatments and shades on the color, translucency, and surface roughness of high-translucency self-glazed zirconia materials is unclear.

Purpose: The purpose of this in vitro study was to investigate the effects of different external surface treatments (self-glazed, milled, polished, and glazed), intaglio surface treatments (milled and airborne-particle abraded), and shades (A1 and A3 shades) on the color, translucency, and surface roughness of high-translucency self-glazed zirconia materials, as well as the correlations among optical parameters, translucency, and surface roughness.

Material And Methods: Eighty shade A1 and 80 shade A3 disks were fabricated with a thickness of 0.80 ±0.02 mm and divided into 16 groups (n=10). Different external and intaglio surface treatments were applied to the specimens. CIELab values were measured with a spectrophotometer, and color differences (ΔE) and relative translucency parameter (RTP) were calculated. Total transmittance (Tt%) and reflectance (R%) were tested with a spectrophotometer equipped with an integrating sphere. Surface roughness (Ra and Rz) (μm) was measured with a noncontact 3-dimensional laser scanning microscope. One specimen from each group was subjected to scanning electron microscope (SEM) examination. Data were analyzed with ANOVA and the Tukey post hoc test. The correlation among optical parameters, translucency, and surface roughness was investigated by using Pearson correlation analysis (α=.05).

Results: The effects of external surface treatments, intaglio airborne-particle abrasion, and shades on ΔE, RTP, and Ra values of the disks were significantly different (P<.001). The smoothest external polishing surface had the greatest RTP and color difference (P<.001). Shade A3 disks had lower RTP and Tt% values than shade A1 disks (P<.001). ΔE had a highly positive relationship with the RTP (A1: r=0.884, P<.001; A3: r=0.859, P<.001). SEM images demonstrated that surface treatments affected the surface texture of monolithic zirconia ceramics.

Conclusions: Different surface treatments affected the surface roughness, translucency, and final color of zirconia materials. The smoothest external polishing surface had the greatest RTP and color difference. Different shades influenced the translucency, as the darker the disk shade, the lower the translucency. The RTP was appropriate as an auxiliary indicator for evaluating the color of a dental ceramic.
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http://dx.doi.org/10.1016/j.prosdent.2022.05.014DOI Listing
July 2022

Myocardial Injury Caused by Chronic Alcohol Exposure-A Pilot Study Based on Proteomics.

Molecules 2022 Jul 3;27(13). Epub 2022 Jul 3.

Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China.

Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM.
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http://dx.doi.org/10.3390/molecules27134284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268295PMC
July 2022

Differentiation of Cerebral Dissecting Aneurysm from Hemorrhagic Saccular Aneurysm by Machine-Learning Based on Vessel Wall MRI: A Multicenter Study.

J Clin Med 2022 Jun 23;11(13). Epub 2022 Jun 23.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.

The differential diagnosis of a cerebral dissecting aneurysm (DA) and a hemorrhagic saccular aneurysm (SA) often depends on the intraoperative findings; thus, improved non-invasive imaging diagnosis before surgery is essential to distinguish between these two aneurysms, in order to provide the correct formulation of surgical procedure. We aimed to build a radiomic model based on high-resolution vessel wall magnetic resonance imaging (VW-MRI) and a machine-learning algorithm. In total, 851 radiomic features from 146 cases were analyzed retrospectively, and the ElasticNet algorithm was used to establish the radiomic model in a training set of 77 cases. A clinico-radiological model using clinical features and MRI features was also built. Then an integrated model was built by combining the radiomic model and clinico-radiological model. The area under the ROC curve (AUC) was used to quantify the performance of models. The models were evaluated using leave-one-out cross-validation in a training set, and further validated in an external test set of 69 cases. The diagnostic performance of experienced radiologists was also assessed for comparison. Eight features were used to establish the radiomic model, and the radiomic model performs better (AUC = 0.831) than the clinico-radiological model (AUC = 0.717), integrated model (AUC = 0.813), and even experienced radiologists (AUC = 0.801). Therefore, a radiomic model based on VW-MRI can reliably be used to distinguish DA and hemorrhagic SA, and, thus, be widely applied in clinical practice.
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http://dx.doi.org/10.3390/jcm11133623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267569PMC
June 2022

Tensorized Bipartite Graph Learning for Multi-View Clustering.

IEEE Trans Pattern Anal Mach Intell 2022 Jul 4;PP. Epub 2022 Jul 4.

Despite the impressive clustering performance and efficiency in characterizing both the relationship between the data and cluster structure, most existing graph-based multi-view clustering methods still have the following drawbacks. They suffer from the expensive time burden due to both the construction of graphs and eigen-decomposition of Laplacian matrix. Moreover, none of them simultaneously considers the similarity of inter-view and similarity of intra-view. In this article, we propose a variance-based de-correlation anchor selection strategy for bipartite construction. The selected anchors not only cover the whole classes but also characterize the intrinsic structure of data. Following that, we present a tensorized bipartite graph learning for multi-view clustering (TBGL). Specifically, TBGL exploits the similarity of inter-view by minimizing the tensor Schatten p-norm, which well exploits both the spatial structure and complementary information embedded in the bipartite graphs of views. We exploit the similarity of intra-view by using the l-norm minimization regularization and connectivity constraint on each bipartite graph. So the learned graph not only well encodes discriminative information but also has the exact connected components which directly indicates the clusters of data. Moreover, we solve TBGL by an efficient algorithm which is time-economical and has good convergence. Extensive experimental results demonstrate that TBGL is superior to the state-of-the-art methods. Codes and datasets are available: https://github.com/xdweixia/TBGL-MVC.
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http://dx.doi.org/10.1109/TPAMI.2022.3187976DOI Listing
July 2022

Nonlinear waveform distortion: Assessment and detection of clipping on speech data and systems.

Speech Commun 2021 Nov 12;134:20-31. Epub 2021 Aug 12.

Center for Robust Speech Systems (CRSS), Erik Jonsson School of Engineering, University of Texas at Dallas, Richardson, TX, USA.

Speech, speaker, and language systems have traditionally relied on carefully collected speech material for training acoustic models. There is an enormous amount of freely accessible audio content. A major challenge, however, is that such data is not professionally recorded, and therefore may contain a wide diversity of background noise, nonlinear distortions, or other unknown environmental or technology-based contamination or mismatch. There is a crucial need for automatic analysis to screen such unknown datasets before acoustic model development training, or to perform input audio purity screening prior to classification. In this study, we propose a waveform based clipping detection algorithm for naturalistic audio streams and examine the impact of clipping at different severities on speech quality measurements and automatic speaker recognition systems. We use the TIMIT and NIST SRE08 corpora as case studies. The results show, as expected, that clipping introduces a nonlinear distortion into clean speech data, which reduces speech quality and performance for speaker recognition. We also investigate what degree of clipping can be present to sustain effective speech system performance. The proposed detection system, which will be released, could contribute to massive new audio collections for speech and language technology development (e.g. Google Audioset (Gemmeke et al., 2017), CRSS-UTDallas Apollo Fearless-Steps (Yu et al., 2014) (19,000 h naturalistic audio from NASA Apollo missions)).
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http://dx.doi.org/10.1016/j.specom.2021.07.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246086PMC
November 2021

Predictive Value of Systemic Immune-Inflammation index and Neutrophil-to-Lymphocyte Ratio in Patients with Severe COVID-19.

Clin Appl Thromb Hemost 2022 Jan-Dec;28:10760296221111391

Department of Laboratory Medicine, Jingzhou Central Hospital; Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, Hubei, China.

It was initially reported that a novel coronavirus (COVID-19) had been identified in Wuhan, China, in December 2019.To date, COVID-19 is still threatening all humanity and has affected the public healthcare system and the world economic situation. Neutrophil-to-lymphocyte ratio (NLR) has also been demonstrated that associated with severity of COVID-19, but little is known about systemic immune-inflammation index (SII) relation with COVID-19. One hundred and twenty-five patients with diagnosed COVID-19 including non-severe cases (n = 77) and severe cases (n = 48) were enrolled in this study. Each patient of clinical characteristic information, blood routine parameters, and the haemogram-derived ratios were collected, calculated, and retrospectively analyzed. Receiver operating characteristics (ROC) was performed to investigate whether these parameters could be used to the predictive value of patients with severe COVID-19. White blood cell count (WBC), neutrophil count (NEU), red cell volume distribution width (RDW), NLR, Platelet to lymphocyte ratio (PLR), neutrophil-to-platelet ratio (NPR), and SII were significantly higher in the severe groups than in the non-severe group (p < 0.01).Conversely, the severe group had a markedly decreased lymphocyte count, basophil (Baso#) count, red blood cell count (RBC), Hemoglobin (HGB), hematocrit (HCT), and lymphocyte-to-monocyte ratio (LMR) (P < 0.01).ROC curve analysis showed the AUC, optimal cut-off value, sensitivity, specificity of NLR and SII to early predict severe-patients with COVID-19 were 0.867, 7.25, 70.83%, 92.21% and 0.860, 887.20, 81.25%, 81.82%, respectively. The results suggest that the SII and NLR is a potential new diagnosed biomarker in severe-patients with COVID-19.
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http://dx.doi.org/10.1177/10760296221111391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247370PMC
June 2022

Optimizing the Construction and Update Strategies for the Genomic Selection of Pig Reference and Candidate Populations in China.

Front Genet 2022 8;13:938947. Epub 2022 Jun 8.

Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Optimizing the construction and update strategies for reference and candidate populations is the basis of the application of genomic selection (GS). In this study, we first simulated1200-purebred-pigs population that have been popular in China for 20 generations to study the effects of different population sizes and the relationship between individuals of the reference and candidate populations. The results showed that the accuracy was positively correlated with the size of the reference population within the same generation ( = 0.9366, < 0.05), while was negatively correlated with the number of generation intervals between the reference and candidate populations ( = -0.9267, < 0.01). When the reference population accumulated more than seven generations, the accuracy began to decline. We then simulated the population structure of 1200 purebred pigs for five generations and studied the effects of different heritabilities (0.1, 0.3, and 0.5), genotyping proportions (20, 30, and 50%), and sex ratios on the accuracy of the genomic estimate breeding value (GEBV) and genetic progress. The results showed that if the proportion of genotyping individuals accounts for 20% of the candidate population, the traits with different heritabilities can be genotyped according to the sex ratio of 1:1male to female. If the proportion is 30% and the traits are of low heritability (0.1), the sex ratio of 1:1 male to female is the best. If the traits are of medium or high heritability, the male-to-female ratio is 1:1, 1:2, or 2:1, which may achieve higher genetic progress. If the genotyping proportion is up to 50%, for low heritability traits (0.1), the proportion of sows from all genotyping individuals should not be less than 25%, and for the medium and high heritability traits, the optimal choice for the male-to-female ratio is 1:1, which may obtain the greatest genetic progress. This study provides a reference for determining a construction and update plan for the reference population of breeding pigs.
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http://dx.doi.org/10.3389/fgene.2022.938947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213789PMC
June 2022

Molecular Sieving of Propylene from Propane in Metal-Organic Framework-Derived Ultramicroporous Carbon Adsorbents.

ACS Appl Mater Interfaces 2022 Jul 24;14(26):30443-30453. Epub 2022 Jun 24.

Key Laboratory of Biomass Chemical Engineering of ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, 38 Zheda Road, Hangzhou 310027, P. R. China.

The development of adsorption-based separation processes alternative to the energy-intensive cryogenic distillation for a mixture of propylene and propane remains essential but challenging in gas industries. Molecular sieving separation of CH/CH on stable carbon adsorbents appeals to be promising, while it is quite challenging to realize due to the random distributions and arrangements of the internal pores in common carbons. Herein, a series of polysaccharide-based CD-MOF-derived ultramicroporous carbon adsorbents with their pore size tuned at a subangstrom level were prepared. Molecular sieving separation of CH/CH was realized on the optimal C-CDMOF-2-700 owing to the delicate structure with an appropriate pore size (5.0 Å). Besides, C-CDMOF-2-700 exhibited a high CH uptake of 1.97 mmol g under ambient conditions. An ultrahigh uptake ratio of CH/CH at 1.0 kPa (403) was also achieved, outperforming all reported adsorbents. Kinetic adsorption tests and breakthrough experiments further demonstrate this well-designed carbon adsorbent to be promising in industrial CH/CH separation.
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http://dx.doi.org/10.1021/acsami.2c09189DOI Listing
July 2022

The Influence of Erotic Stimulation on Brand Preference of Male and Female Consumers: From the Perspective of Human Reproductive Motives.

Front Psychol 2022 7;13:848864. Epub 2022 Jun 7.

College of Management, Shenzhen University, Shenzhen, China.

Based on the theory of human reproductive motives, this study discusses how erotic stimulation can help activate male and female consumers' reproductive motives (i.e., mate-attraction and mate-retention motives), influencing product purchase intention and brand personality perception. Specifically, the results of four experiments reveal that when a man receives erotic stimulation from women, his mate-attraction motive is activated. Consequently, he perceives that the brand personality of his possessions is more rugged, and his preference for products with a rugged brand personality is stronger. Unlike male consumers, when a heterosexual woman in an intimate romantic relationship receives erotic stimulation from other women, her mate-retention motive is activated. As a result, she perceives that the brand personality of her partner's possessions is sincerer and prefers to purchase products with a sincere brand personality for her partner. This effect will not occur when a product is owned by her male colleague than her partner.
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http://dx.doi.org/10.3389/fpsyg.2022.848864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210950PMC
June 2022

Oxidative degradation of pre-oxidated polystyrene plastics by dye decolorizing peroxidases from Thermomonospora curvata and Nostocaceae.

J Hazard Mater 2022 08 2;436:129265. Epub 2022 Jun 2.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address:

Biodegradation of PS has attracted lots of public attentions due to its environmental friendliness. However, no specific PS degrading enzyme has been identified yet. Dye decolorizing peroxidases (DyPs) are heme-containing peroxidases named for the ability to degrade a variety of organic dyes. Herein, the abilities of two DyPs from Thermomonospora curvata (TcDyP) and Nostocaceae (AnaPX) to degrade PS were evaluated. Preoxidation methods by ultraviolet (UV) irradiation and chemical oxidants were developed to initially activate C-C bonds in the PS skeleton. DyPs degradation caused obvious etching and enhanced hydrophilicity of UV-PS films, and also generated new CO and C-OH groups. The cleavage of activated C-C bonds by DyPs was experimentally proven by analyzing the degradation products of UV-PS and model substrates. Furthermore, better pre-oxidation was obtained by using chemical oxidants KMnO/HSO and CPBA to oxidize PS materials in dissolved state. And AnaPX exhibited stronger degradation effects on KMnO/HSO-PS and CPBA-PS by causing greater changes in functional groups CO, C-O, -OH groups and substituted benzenes and higher molecular weight reductions of 19.7% and 31.0%, respectively. To our knowledge, this is the first report on the identification of PS-degrading enzymes that provides experimental evidence.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129265DOI Listing
August 2022

Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm.

Front Plant Sci 2022 2;13:885418. Epub 2022 Jun 2.

Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, China.

Oil palm ( Jacq.) is a tropical woody oil crop of the palm family and is known as "the oil king of the world," but its palm oil contains about 50% palmitic acid, which is considered unhealthy for humans. Intron polymorphisms (IP) are highly efficient and easily examined molecular markers located adjacent to exon regions of functional genes, thus may be associated with targeted trait variation. In order to speed up the breeding of oil palm fatty acid composition, the current study identified a total of 310 introns located within 52 candidate genes involved in fatty acid biosynthesis in the oil palm genome. Based on the intron sequences, 205 primer pairs were designed, 64 of which showed polymorphism among 70 oil palm individuals. Phenotypic variation of fatty acid content in the 70 oil palm individuals was also investigated. Association analysis revealed that 13 IP markers were significantly associated with fatty acid content variation, and these IP markers were located on chromosomes 2, 5, 6, 8, 9, and 10 of oil palm. The development of such IP markers may be useful for the genetic improvement of fatty acid composition in oil palm.
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http://dx.doi.org/10.3389/fpls.2022.885418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201816PMC
June 2022
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