Publications by authors named "Wei Wu"

4,521 Publications

  • Page 1 of 1

Biofilms in wound healing: A bibliometric and visualised study.

Int Wound J 2022 Jun 29. Epub 2022 Jun 29.

Department of Breast Thyroid Surgery, The Third Xiangya Hospital, Central South University, Changsha, China.

Bibliometric analyses are often used as a means of visualising the knowledge base and associated trends and patterns in a target scientific field based on a quantitative review of the corresponding literature. In this study, we explore the current status of research pertaining to biofilms in wound healing and elucidate trends in this research space. Through this process, we gain insight into findings from papers indexed in the Web of Science Core Collection. These references were then analysed and plotted using Microsoft Excel 2019, VOSviewer, and CiteSpace V. The results provide a fresh perspective regarding global trends and hotspots in biofilm-related wound healing research. These findings also offer a foundation that researchers can use to identify active hotspots of scientific interest to guide further research endeavours.
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http://dx.doi.org/10.1111/iwj.13878DOI Listing
June 2022

The Multidimensional Burden of Atopic Dermatitis Among Adults: Results From a Large National Survey.

JAMA Dermatol 2022 Jun 29. Epub 2022 Jun 29.

Temerty Faculty of Medicine, University of Toronto and Women's College Research Institute, Women's College Hospital, Toronto, Ontario, Canada.

Importance: Atopic dermatitis (AD) is long term and burdensome. Studies investigating disease burden in adults are limited in scope with gaps in understanding of the adult patient lived experience.

Objective: To describe the multidimensional burden of AD among mainly US adults.

Design, Setting, And Participants: This survey study for an externally led patient-focused drug development meeting with the US Food and Drug Administration on adult patients with AD was conducted between August 1, 2019, and October 11, 2019. Data were analyzed betwean March 26, 2021, and June 29, 2021.

Main Outcomes And Measures: We used multivariable ordinal regression to assess associations between demographic and clinical variables and patient-reported overall AD impact scores (ordinal scale from 1 [no impact] to 5 [significant impact]).

Results: Among 1065 survey respondents, 114 (11%) were aged 18 to 24 years, 235 (22%) were 25 to 34 years, 242 (23%) were 35 to 50 years, 288 (27%) were 51 to 64 years, and 186 (17%) were aged 65 years or older; 881 (83%) were women. Four hundred eighty-nine (46%) participants reported low-moderate AD impact scores (2-3), 544 (51%) reported high-significant impact scores (4-5), whereas 32 (3%) reported no association of AD with disease burden (impact score, 1). Variables strongly associated with overall impact scores were current AD severity (moderate: OR, 4.13; 95% CI, 2.94-5.79; severe: OR, 13.63; 95% CI, 8.65-21.50 vs mild), and time spent managing AD (11-20 hours: OR, 2.67; 95% CI, 1.77-4.03, ≥21 hours: OR, 5.34; 95% CI, 3.22-8.85, vs <5 hours).

Conclusions And Relevance: In this survey study, AD severity and time spent managing symptoms showed the strongest associations with disease burden. This analysis highlights the multidimensional burden of AD in adults and emphasizes the need for more effective treatment strategies that reduce the time patients spend managing their AD.
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http://dx.doi.org/10.1001/jamadermatol.2022.1906DOI Listing
June 2022

Comparative dietary effectiveness of a modified government-recommended diet with avoidance of ultra-processed foods on weight and metabolic management in children and adolescents: An open-label, randomized study.

Asia Pac J Clin Nutr 2022 ;31(2):282-293

Departments of Endocrinology, the Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, China. Email:

Background And Objectives: Childhood obesity is rapidly rising in China and effective diet interventions are needed. Here, we determine whether the Chinese government-recommended diet (GRD) or a modified diet of further restriction of sugar and ultra-processed food but without energy restriction, minimally processed diet (MPD) is effective on weight loss in children and adolescents with obesity/overweight.

Methods And Study Design: This open-label, randomized study included 60 children and adolescents between 5-18 years old with overweight/obesity. Participants were randomized 1:1 to the GRD or MPD and self-managed at home for 12 weeks. Both groups received general recommendations in physical activities. The changes were evaluated in body weight, fasting glucose and insulin, lipid metabolism and serum uric acid between baseline and week 12.

Results: The results indicated great reductions by time for BMI, BMI z-score, fat mass percentage and fat mass index in both groups. An obvious decrease by time for weight was found in the MPD group (p<0.001) as well as fasting glucose (p=0.005), fasting insulin (p=0.001), total cholesterol (p=0.007) and serum uric acid (p=0.006). As for the amount of visceral fat, greater reduction by time was observed in MPD group compared with GRD group.

Conclusions: A 12-week self-intervention combining the Chinese government-recommended diet with physical activities was effective on weight loss in children and adolescents with overweight/obesity. The minimally processed diet was more effective on decreasing visceral fat mass and may be beneficial to improving insulin resistance. Further studies are required to assess long-term outcomes of the general public.
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http://dx.doi.org/10.6133/apjcn.202206_31(2).0014DOI Listing
January 2022

Hypochlorous Acid-Activated Multifunctional Fluorescence Platform for Depression Therapy and Antidepressant Efficacy Evaluation.

Anal Chem 2022 Jun 28. Epub 2022 Jun 28.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.

Current diagnosis of depression rests on the symptoms, so it still lacks objective criteria. Meanwhile, existing treatment of depression is dominated by antidepressants, which produce troublesome side effects and usually require months to achieve effect. Therefore, more reliable diagnostic criteria and effective therapy are urgently needed. Some core hallmarks in the etiology of depression have been established, including declined neurotransmitters and inflammatory responses, manifesting in oxidative stress. Thus, we fabricated a HClO-triggered multifunctional fluorescence platform (MB-Rs) for simultaneous neurotransmitter/antidepressant delivery and efficacy evaluation. In MB-Rs, although a urea linkage could be specifically cut off by HClO, methylene blue (MB) endowed with excellent anti-inflammatory and optical properties was covalently linked with neurotransmitters (dopamine or 5-hydroxytryptamine) or antidepressants (fluoxetine). Encountering excess HClO in the brain of mice with depression, MB-Rs released corresponding antidepressants and MB with anti-inflammatory and bright fluorescence. By relieving oxidative stress, inflammation, and coinstantaneous increasing neurotransmitters, MB-Rs elicited better antidepressant response and fewer side effects compared with clinical antidepressants. Furthermore, MB-Rs successfully evaluated the efficacy of antidepressants in mice based on HClO-induced fluorescence. Therefore, this work provides a promising platform for depression diagnosis and treatment.
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http://dx.doi.org/10.1021/acs.analchem.2c01639DOI Listing
June 2022

Clinical Significance of Dual-Block Elastic Stain Evaluating Visceral Pleural Invasion in Peripheral Non-Small Cell Lung Cancer.

Int J Surg Pathol 2022 Jun 27:10668969221098089. Epub 2022 Jun 27.

Department of Pathology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Visceral pleural invasion (VPI) is a critical component in the staging of peripheral non-small cell lung carcinoma (NSCLC). We aim to investigate whether dual-block elastic stain increases visceral pleural invasion positivity compared with single-block elastic stain. We further analyze the potential predictors of visceral pleural invasion. 8419 peripheral NSCLC patients (including 6008 patients with tumor size≤3 cm in stage I) were divided into a cohort using one paraffin block (single-block group, n = 5184) and a cohort using dual paraffin blocks (dual-block group, n = 3235) for elastic stain. The VPI-positive rate demonstrated by the dual-block elastic stains group was significantly higher than that of the single-block elastic stain group (17.7% (573/3235) versus 9.1% (474/5184), respectively,  < .001). The presence of visceral pleural invasion in T1 (≤3 cm) patients detected by single- and dual-block elastic stain was 6.3% (235/3730) and 12.0% (273/2278), respectively ( < .001). 5.7% of T1 patients (stage IA) were additionally upstaged to T2a (stage IB) by dual-block elastic stain. However, the incidence of visceral pleural invasion in pT2a patients showed no significant difference between the single-block group and the dual-block group (16.8% vs. 17.1%,  = .916). Lymphovascular invasion, lymph node metastasis, dedifferentiated carcinomas, the presence of spread through airspaces (STAS) and a poorly differentiated adenocarcinomatous growth pattern could be significant predictors of visceral pleural invasion ( < .001). Our results indicate that using dual-block elastic stain identifies more visceral pleural invasion positive T1 NSCLC patients who are upstaged to T2a, and who could benefit from optimal management post-operatively. The application of dual-block elastic stain is an efficient and practical method to detect visceral pleural invasion status.
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http://dx.doi.org/10.1177/10668969221098089DOI Listing
June 2022

Targeted peptide-modified oxidized mesoporous carbon nanospheres for chemo-thermo combined therapy of ovarian cancer .

Drug Deliv 2022 Dec;29(1):1947-1952

Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai, China.

Ovarian cancer remains one of serious hazards to human health due to many drawbacks of existing available treatment options. In this study, a multifunctional chemo-thermo combined therapy nanoplatform (OMCNPID) was successfully prepared, which is composed of IP peptide as a targeting moiety to interleukin-6 receptors (IL-6Rs), oxidized mesoporous carbon nanospheres (OMCN) as a near infrared (NIR)-triggered drug carrier and doxorubicin (DOX) as a chemotherapeutic drug and fluorescent agent. The synthesized multifunctional nanoplatform displayed high storage capacity for drugs and excellent photothermal properties. Besides, DOX was rapidly released from OMCNPID at the condition of low pH and NIR laser irradiation due to the dissociation of DOX from graphitic cores of OMCN. experimental results verified that OMCNPID could be markedly taken up by SKOV-3 monolayer cells and tumor spheroids, and revealed a remarkable synergistic chemo-photothermal effect against ovarian cancer. All the results demonstrated that OMCNPID is a pH/NIR dual-stimulus responsive nanoplatform and can achieve efficient chemo-thermo combined therapy.
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http://dx.doi.org/10.1080/10717544.2022.2089298DOI Listing
December 2022

Transcriptomic Analysis of Dysregulated Genes of the nDNA-mtDNA Axis in a Mouse Model of Dilated Cardiomyopathy.

Front Genet 2022 8;13:921610. Epub 2022 Jun 8.

Key Laboratory of Environment and Genes Related to Diseases, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Mitochondrial dysfunction is implicated in the development of cardiomyopathy and heart failure. Transcription of mitochondrial DNA (mtDNA) encoded genes and subsequent protein synthesis are tightly regulated by nuclear DNA (nDNA) encoded proteins forming the nDNA-mtDNA axis. The scale of abnormalities in this axis in dilated cardiomyopathy (DCM) is unclear. We previously demonstrated, in a mouse DCM model with cardiac Mst1 overexpression, extensive downregulation of mitochondrial genes and mitochondrial dysfunction. Using the pre-acquired transcriptome sequencing database, we studied expression of gene sets of the nDNA-mtDNA axis. Using RNA-sequencing data from DCM hearts of mice at early and severe disease stages, transcriptome was performed for dysregulated nDNA-encoded gene sets that govern mtDNA transcription and protein synthesis. To validate gene data, expression of a panel of proteins was determined by immunoblotting. Relative to littermate controls, DCM hearts showed significant downregulation of all mtDNA encoded mRNAs, as well as mtDNA transcriptional activators. Downregulation was also evident for gene sets of mt-rRNA processing, aminoacyl-tRNA synthases, and mitoribosome subunits for protein synthesis. Multiple downregulated genes belong to mitochondrial protein-importing machinery indicating compromised importing of proteins for mtDNA transcription and translation. Diverse changes were genes of mtRNA-binding proteins that govern maturation and stability of mtDNA-derived RNAs. Expression of mtDNA replicome genes was largely unchanged. These changes were similarly observed in mouse hearts at early and severe stages of DCM. Transcriptome revealed in our DCM model dysregulation of multiple gene sets of the nDNA-mtDNA axis, that is, expected to interfere with mtDNA transcription and protein synthesis. Dysfunction of the nDNA-mtDNA axis might contribute to mitochondrial dysfunction and ultimately development of DCM.
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http://dx.doi.org/10.3389/fgene.2022.921610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214240PMC
June 2022

Differences in Multicomponent Pharmacokinetics, Tissue Distribution, and Excretion of Tripterygium Glycosides Tablets in Normal and Adriamycin-Induced Nephrotic Syndrome Rat Models and Correlations With Efficacy and Hepatotoxicity.

Front Pharmacol 2022 9;13:910923. Epub 2022 Jun 9.

Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, China.

Tripterygium glycosides tablets (TGT) are widely used for treating nephrotic syndrome (NS), but hepatotoxicity is frequently reported. The presence of underlying disease(s) can alter the disposition of drugs and affect their efficacy and toxicity. However, no studies have reported the impact of NS on the ADME profiles of TGT or its subsequent impact on the efficacy and toxicity. Thus, the efficacy and hepatotoxicity of TGT were evaluated in normal and NS rats after oral administration of TGT (10 mg/kg/day) for 4 weeks. The corresponding ADME profiles of the six key TGT components (triptolide (TPL), wilforlide A (WA), wilforgine (WFG), wilfortrine (WFT), wilfordine (WFD), and wilforine (WFR)) were also measured and compared in normal and NS rats after a single oral gavage of 10 mg/kg TGT. Canonical correlation analysis (CCA) of the severity of NS and the exposure of the six key TGT components was performed to screen the anti-NS and hepatotoxic material bases of TGT. Finally, the efficacy and hepatotoxicity of the target compounds were evaluated . The results showed that TGT decreased the NS symptoms in rats, but caused worse hepatotoxicity under the NS state. Significant differences in the ADME profiles of the six key TGT components between the normal and NS rats were as follows: higher plasma and tissue exposure, lower urinary and biliary excretion, and higher fecal excretion for NS rats. Based on CCA and verification, TPL, WA, WFG, WFT, WFD, and WFR were identified as the anti-NS material bases of TGT, whereas TPL, WFG, WFT, and WFD were recognized as the hepatotoxic material bases. In conclusion, NS significantly altered the ADME profiles of the six key TGT components detected in rats, which were related to the anti-NS and hepatotoxic effects of TGT. These results are useful for the rational clinical applications of TGT.
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http://dx.doi.org/10.3389/fphar.2022.910923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221999PMC
June 2022

Perfluoroalkyl substance pollution: detecting and visualizing emerging trends based on CiteSpace.

Environ Sci Pollut Res Int 2022 Jun 26. Epub 2022 Jun 26.

Lake Basin Management, National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, Chinese Research Academy of Environmental Sciences, Beijing, 100012, People's Republic of China.

In recent years, perfluoroalkyl substances (PFASs) have been detected in all kinds of environmental media and can harm animals and human beings. They have attracted the attention of environmental workers worldwide and have become another research hotspot in the field of environment. However, analyses of PFASs have seldom been studied systematically. Therefore, this study summarizes the available data in 6756 publications (2000-2022) using the CiteSpace software to provide insights into the specific characteristics of PFASs and consequently shows global development trends that scientists can use for establishing future research directions. As opposed to traditional review articles by experts, this study provides a new method for quantitatively visualizing information about the development of this field over the past 23 years. Results show that the countries with more research in this field are mainly the USA and China. The research on PFASs is mainly concentrated in environmental sciences and ecology. Zhanyun Wang and Robert C. Buck's research has the highest influence rate in this field, and their research group is worthy of attention. Through the analysis of hot keywords, we conclude that the research hotspots are mainly focused on PFASs' transmission media and pathways, human exposure and the mechanism of toxicity, and degradation and remediation measures. Collectively these results indicate the major themes of PFAS research are as follows: (1) transmission media and pathways, (2) human exposure and the mechanism of toxicity, (3) degradation and remediation measures. This study maps the major research domains of PFAS research; explanations and implications of the findings are discussed; and emerging trends highlighted.
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http://dx.doi.org/10.1007/s11356-022-20756-3DOI Listing
June 2022

Effect of Boron Doping on the Interlayer Spacing of Graphite.

Materials (Basel) 2022 Jun 13;15(12). Epub 2022 Jun 13.

College of Materials Science and Engineering, Hunan University, Changsha 410082, China.

Boron-doped graphite was prepared by the heat treatment of coke using BC powder as a graphitization catalyst to investigate the effects of the substitutional boron atoms on the interlayer spacing of graphite. Boron atoms can be successfully incorporated into the lattice of graphite by heat treatment, resulting in a reduction in the interlayer spacing of graphite to a value close to that of ideal graphite (0.3354 nm). With an increase in the catalyst mass ratio, the content of substituted boron in the samples increased significantly, causing a decrease in the interlayer spacing of the boron-doped graphite. Density functional theory calculations suggested that the effects of the substitutional boron atoms on the interlayer spacing of the graphite may be attributed to the transfer of Π electrons between layers, the increase in the electrostatic surface potential of the carbon layer due to the electron-deficient nature of boron atoms, and Poisson contraction along the c-axis.
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http://dx.doi.org/10.3390/ma15124203DOI Listing
June 2022

Electrochemical Preparation of Hydroxylated Boron Nitride Nanosheets for Solid-State Flexible Supercapacitors Using Deep Eutectic Solvent and Water Mixture as Electrolytes.

Langmuir 2022 Jun 23. Epub 2022 Jun 23.

College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

One-step and efficient preparation of few-layer hydroxylated boron nitride nanosheets (OH-BNNSs) in electrochemical methods is still challenging. Here, we developed an electrolyte composed of a mixture of deep eutectic solvent (DES, choline chloride-urea) and water for electrochemical methods to enhance the exfoliation and oxidation processes, enabling the one-step preparation of OH-BNNSs. It was found that the obtained OH-BNNSs were an average lateral size of 625 nm and thickness of six layers. Furthermore, the OH-BNNSs and DES were added to the poly(vinyl alcohol) (PVA) substrate to prepare composite gel polymer electrolyte (PVA/DES/OH-BNNSs GPE) for solid-state flexible supercapacitor. The OH-BNNSs can effectively shorten the ionic transport distance and enhance ion conductivity. In addition, their excellent mechanical properties can significantly prevent the electrolyte structure from collapsing during reuse. In the meantime, DES was introduced to improve ionic conductivity and broaden the operating voltage window of supercapacitor. As a result, the symmetric solid-state flexible supercapacitor consisting of activated carbon electrodes and PVA/DES/OH-BNNSs GPE appeared a wide voltage window of 2.3 V, high specific capacitance of 151.22 F g at 0.1 A g and remained 98% capacitance after 1500 charge-discharge cycles. This study not only opened a new pathway to efficient exfoliation of insulating layered materials but also found a novel gel polymer electrolyte for solid-state flexible supercapacitors.
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http://dx.doi.org/10.1021/acs.langmuir.2c01153DOI Listing
June 2022

Degradation characteristics of biodegradable film and its effects on soil nutrients in tillage layer, growth and development of taro and yield formation.

AMB Express 2022 Jun 22;12(1):81. Epub 2022 Jun 22.

Special Grain Classics Laboratory, Taizhou Institute of Agricultural Science, Jiangsu Academy of Agricultural Sciences, 56 Autumn Snow Lake Avenue, Taizhou, 225300, China.

This study investigated the degradation characteristics of different biodegradable film and its effects on soil nutrients in tillage layer, growth and development of taro and yield formation. Field experiment with biodegradable films, including poly-(butylene adipate-co-butylene terephthalate) PBAT, (poly-carbon dioxide) PCO2, (poly propylene carbonate) PPC, as well as common mulch film (CK1) and uncovered mulch film (CK2) were conducted on Longxiang taro in 2020 and 2021 respectively. The degradation rate of the three biodegradable films was PBAT > PPC > PCO2. Compared with CK1, the alkali-hydrolyzed N of PBAT at the growth stage and fruiting stage significantly increased in 2020 and 2021, respectively (both, P < 0.05). The average content of available P of PPC at seedling stage was higher than that in PCO2, and CK1 was significantly decreased compared with that in CK2 (all, P < 0.05). The content of soil available K and organic matter in different growth stages of taro in all film mulching treatments were decreased in comparison to CK2. Moreover, compared with CK2, PCO2 biodegradable film significantly increased plant height at seedling and growth stage, stem diameter at growth stage, and leaf area index at fruiting stage (all, P < 0.05). Similarly, the yield of mother and filial bulbs of PPC, PCO2 and PBAT were significantly higher than those of CK2 in 2020 and 2021, respectively (all, P < 0.05). However, no significant differences were found in starch, polysaccharide and protein contents among different treatments. The three biodegradable films, especially PCO2, can significantly affect soil nutrient content, promote plant growth and improve taro yield.
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http://dx.doi.org/10.1186/s13568-022-01420-yDOI Listing
June 2022

Correction: PROTAC mediated FKBP12 degradation enhances Hepcidin expression via BMP signaling without immunosuppression activity.

Signal Transduct Target Ther 2022 Jun 22;7(1):197. Epub 2022 Jun 22.

MOE Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, 100084, Beijing, China.

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http://dx.doi.org/10.1038/s41392-022-01051-6DOI Listing
June 2022

Does high vegetation coverage equal high giant panda density?

Zool Res 2022 Jul;43(4):608-611

College of Life Science and Technology, Central South University of Forestry & Technology, Changsha, Hunan 410004, China.

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http://dx.doi.org/10.24272/j.issn.2095-8137.2022.005DOI Listing
July 2022

Construction of Core-Shell [email protected] for Aqueous Lubrication and Thermal-Responsive Drug Release.

Small 2022 Jun 16:e2202510. Epub 2022 Jun 16.

State Key Laboratory of Solidification Processing, Center of Advanced Lubrication and Seal Materials, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, 710072, P. R. China.

The construction of porous nanocarriers with good lubricating performance and stimuli-responsive drug release is significant for the synergetic therapy of osteoarthritis (OA). Although metal-organic framework nanoparticles (nanoMOFs) as carriers can support drug delivery, achieving the synergy of aqueous lubrication and stimuli-responsive drug release is challenging. In this work, a core-shell [email protected](N-isopropylacrylamide) (PNIPAm) microgel hybrid via one-pot soap-free emulsion polymerization is developed. Programmable growth of the PNIPAm microgel layer on the surface of nanoMOFs is achieved by tuning the concentration of the monomer and the crosslinker in the reaction. Reversible swelling-collapsing behaviors of the hybrid are realized by tuning the temperature below and above the lower critical solution temperature. When used as water lubrication additives, the hybrid enables reductions in both the coefficient of friction and wear volume. In vitro thermal-responsive drug release is demonstrated on the diclofenac sodium-loaded hybrid by controlling the swelling and collapsing states of the PNIPAm nanolayer. Moreover, the good biocompatibility of the hybrid is verified by culturing toward HeLa and BEAS-2B cells. These results establish a [email protected] hybrid that can achieve friction and wear reduction and thermal-responsive drug release.
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http://dx.doi.org/10.1002/smll.202202510DOI Listing
June 2022

The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal model.

BMC Cancer 2022 Jun 17;22(1):663. Epub 2022 Jun 17.

Department of Ultrasound, Peking University Cancer Hospital & Institute, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Beijing, 100142, China.

Background: Direct and indirect effects of radiofrequency ablation (RFA) on tumor microenvironment of the liver tumor have been noted, which was reported to be related to a variety of tyrosine protein kinase or cytokinetic pathway, but have not been thoroughly investigated and conclusive.

Purpose: To elucidate direct and indirect effects of RFA on tumor microenvironment in the liver tumor model, and to explore the role of the specific inhibitor in tumor growth by targeting the key pathway of RFA.

Materials And Methods: One hundred and ten mice with H22 liver tumor were used in animal experiments. Eighty-four mice were randomized into three groups: control, direct RFA and indirect RFA (a block slide was inside the middle of the tumor). The growth rate of the residual tumor after RFA was calculated (n = 8 each group) and the pathologic changes at different time points (6 h, 24 h, 72 h and 7d after RFA) were evaluated (n = 5 in each subgroup). After semi-quantitative analysis of the pathological staining, the most significant marker after RFA was selected. Then, the specific inhibitor (PHA) was applied with RFA and the tumor growth and pathological changes were evaluated and compared with RFA alone. The Kruskal-Wallis test was used for evaluating the significance of different treatments in the pathological positive rate of specific markers in tumor. The two-way analysis of variance was used to determine the significance of treatment in tumor growth or body weight.

Results: The growth rate of the residual tumor in the direct RFA group was faster than the indirect RFA group (P = 0.026). The pathological analysis showed the expression of HSP70 (73 ± 13% vs 27 ± 9% at 24 h, P < 0.001), SMA (70 ± 18% vs 18 ± 7% at 6 h, P < 0.001) and Ki-67 (51 ± 11% vs 33 ± 14% at 7d, P < 0.001) in the direct RFA group was higher than those in the indirect RFA group after RFA. On the other hand, the expression of c-Met (38 ± 11% vs 28 ± 9% at 24 h, P = 0.01), IL-6 (41 ± 10% vs 25 ± 9% at 24 h, P < 0.001) and HIF-α (48 ± 10% vs 28 ± 8% at 24 h, P < 0.001) in the indirect RFA group was higher than those in the direct RFA group. And the expression of c-Met increased mostly in both direct and indirect RFA group compared to the baseline (53 and 65% at 72 h). Then the specific inhibitor of c-Met-PHA was applied with RFA. The growth rate of the tumor was significantly slower in the RFA + PHA group than the RFA alone group (1112.9 ± 465.6 mm vs 2162.7 ± 911.1 mm at day 16, P = 0.02).

Conclusion: Direct and indirect effects of RFA on tumor microenvironment changed at different time points and resulted in increased residual tumor growth in the animal model. It can be potentially neutralized with specific inhibitor of related pathways, such as tyrosine-protein kinase c-Met.
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http://dx.doi.org/10.1186/s12885-022-09730-xDOI Listing
June 2022

Transcription factor TERF1 promotes seed germination under osmotic conditions by activating gibberellin acid signaling.

Plant Sci 2022 Jun 13;322:111350. Epub 2022 Jun 13.

Graduate School of Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South St., Haidian District, Beijing 100081, PR China. Electronic address:

Seed germination is the first step of seedling establishment, which is particularly sensitive to drought stress. Elucidating the mechanism regulating seed germination under drought stress is of great importance. We showed that overexpressing Tomato Ethylene Responsive Factor 1 (TERF1), an ERF transcription factor in the ethylene signaling pathway, significantly reduced seed sensitivity to mannitol treatment during seed germination. Germination assay demonstrated that TERF1 could activate gibberellin acid (GA) signaling pathway independent on GA metabolism during germination. By comparative transcriptome analysis (mannitol vs normal germination condition, mannitol vs mannitol plus paclobutrazol (PAC, an inhibitor of GA biosynthesis)) we identified the genes regulated by TERF1 specifically under mannitol treatment and confirmed that TERF1 could activate GA signaling pathway independent on GA metabolism, which were consistent with the germination assay with mannitol and mannitol plus PAC treatment. Based on sugar, gene expression and germination analysis we proved that TERF1 promoted seed germination through glucose signaling pathway mediated by GA. Thus our study provides an underlying mechanism for activating GA signaling pathway by TERF1 during seed germination under osmotic conditions.
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http://dx.doi.org/10.1016/j.plantsci.2022.111350DOI Listing
June 2022

Tumor Response and Nomogram-Based Prognostic Stratification for Hepatocellular Carcinoma After Drug-Eluting Beads Transarterial Chemoembolization.

J Hepatocell Carcinoma 2022 7;9:537-551. Epub 2022 Jun 7.

Department of Interventional Radiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.

Purpose: To explore the tumor response and propose a nomogram-based prognostic stratification for hepatocellular carcinoma (HCC) after drug-eluting beads transarterial chemoembolization (DEB-TACE).

Patients And Methods: From the database of two centers, patients who received DEB-TACE as an initial treatment were enrolled and divided into the training and validation sets. The tumor response after DEB-TACE was estimated according to the Modified Response Evaluation Criteria in Solid Tumors. Using the independent survival predictors in the training set, a nomogram was constructed and validated internally and externally by measuring concordance index (C-index) and calibration. A prognostic stratification based on the nomogram was established.

Results: A total of 335 patients met the inclusion criteria for the study. Alkaline phosphatase level, tumor maximum diameter, tumor capsule and portal vein invasion were interrelated with the achievement of complete release after DEB-TACE. Alpha-fetoprotein level, Child-Pugh class, tumor maximum diameter, tumor number, tumor extent and portal vein invasion were integrated into the nomogram. The nomogram demonstrated good calibration and discrimination, with C-indexes of 0.735 and 0.854 and higher area under the curve (AUC) than BCLC and CNLC staging systems in the internal and external validation sets. The prognostic stratification classified patients into three different risk groups, which had significant differences in survival, complete release and objective response rate between any two groups ( < 0.05).

Conclusion: The nomogram-based prognostic stratification has a good distinction and may help to identify the patients benefiting from DEB-TACE.
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http://dx.doi.org/10.2147/JHC.S360421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188409PMC
June 2022

Whole-genome sequencing as an alternative to analyze copy number abnormalities in acute myeloid leukemia and myelodysplastic syndrome.

Leuk Lymphoma 2022 Jun 11:1-10. Epub 2022 Jun 11.

Central Laboratory, The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, China.

Copy number aberrations (CNA) are the core determinants for diagnosis, risk stratification and prognosis in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). In this study, a shallow whole-genome sequencing-based assay, LeukoPrint, was utilized to depict genomic CNA profiles from the bone marrow of 137 newly diagnosed AML/MDS patients. It demonstrated 98.1% concordance of CNA profiles with cytogenetics and/or fluorescence in situ hybridization (FISH). It is advantageous in detecting CNAs of short segments (1 Mb) and from samples with low leukemic cell content, more accurate for describing complex karyotypes and less confounded by subjective bias. LeukoPrint improved the overall diagnostic yield by redefining the risk categories for 16 patients by presenting new information. In summary, LeukoPrint provided an automated, convenient, and cost-effective approach to describe genomic CNA profiles. It brought greater diagnostic yield and risk stratification information by incorporating into the routine cytogenetics based on the CNA-related criteria of standard ELN/IPSS-R guidelines.
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http://dx.doi.org/10.1080/10428194.2022.2080821DOI Listing
June 2022

Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning.

Front Cell Dev Biol 2022 26;10:869531. Epub 2022 May 26.

Department of Gynecologic Oncology, Zhongshan Hospital, Fudan University, Shanghai, China.

Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized in cells remain incompletely understood. Here, we identify potential kinase targets that participate in nuclear F-actin polymerization in ovarian cancer cells using small-molecule inhibitor library screening in combination with a deep learning approach. The analysis of the targets of the inhibitors used in this study suggest that the PI3K-AKT pathway are involved in regulating nuclear F-actin organization in ovarian cancer cells. Our work lays the foundation for uncovering the important roles of nuclear F-actin in the context of ovarian cancer, and for understanding how nuclear F-actin structures are organized.
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http://dx.doi.org/10.3389/fcell.2022.869531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178185PMC
May 2022

Novel HIVEP1-ALK fusion in a patient with lung adenocarcinoma demonstrating sensitivity to alectinib: a case report.

Transl Lung Cancer Res 2022 May;11(5):902-909

Department of Thoracic Medical Oncology, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, China.

Background: Anaplastic lymphoma kinase (ALK) fusion is an important oncogenic driver in non-small cell lung cancer (NSCLC). Reports on the intergenic region (IGR) as an ALK fusion partner are rare. Here, we report the case of a patient with advanced NSCLC harboring a human immunodeficiency virus type I enhancer binding protein 1 (HIVEP1)-ALK fusion that responded effectively to alectinib.

Case Description: A 60-year-old non-smoking male was referred with a 3-month history of productive cough secondary to lung adenocarcinoma metastatic to mediastinal lymph nodes, brain, liver, and bone (T2N3M1c, stage IVB). Next-generation sequencing identified an IGR (upstream HIVEP1-) ALK fusion, and immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) results were consistent with an ALK-positive tumor. The patient was subsequently started on alectinib, with no obvious adverse reaction. After 1 month of therapy, the patient achieved significantly remission of the clinical symptoms and had led to an ongoing partial response (PR) lasting >33 months.

Conclusions: Our experience highlights the efficacy of alectinib in a patient with HIVEP1-ALK fusion positive NSCLC with multiple metastases including brain disease, and the need for multiple genetic testing methods to verify the oncogenicity of ALK fusions prior to treatment. It could provide useful guidance for the treatment of similar cases in the future.
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http://dx.doi.org/10.21037/tlcr-22-288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186176PMC
May 2022

Inhibitory activity of medicinal mushroom on colorectal cancer by attenuating inflammation.

Precis Clin Med 2021 Dec 28;4(4):231-245. Epub 2021 Aug 28.

Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA.

The medicinal mushroom (GL, Reishi or Lingzhi) exhibits an inhibitory effect on cancers. However, the underlying mechanism of the antitumor activity of GL is not fully understood. In this study, we characterized the gene networks regulated by a commercial product of GL containing a mixture of spores and fruiting bodies namely "GLSF", in colorectal carcinoma. We found that co-administration of GLSF extract at non-toxic concentrations significantly potentiated growth inhibition and apoptosis induced by paclitaxel in CT26 and HCT-15 cells. GLSF inhibited NF-κB promoter activity in HEK-293 cells but did not affect the function of P-glycoprotein in K562/DOX cells. Furthermore, we found that when mice were fed a modified diet containing GLSF for 1 month prior to the CT26 tumor cell inoculation, GLSF alone or combined with Nab-paclitaxel markedly suppressed tumor growth and induced apoptosis. RNA-seq analysis of tumor tissues derived from GLSF-treated mice identified 53 differentially expressed genes compared to normal tissues. Many of the GLSF-down-regulated genes were involved in NF-κB-regulated inflammation pathways, such as IL-1β, IL-11 and Cox-2. Pathway enrichment analysis suggested that several inflammatory pathways involving leukocyte migration and adhesion were most affected by the treatment. Upstream analysis predicted activation of multiple tumor suppressors such as α-catenin and TP53 and inhibition of critical inflammatory mediators. "Cancer" was the major significantly inhibited biological effect of GLSF treatment. These results demonstrate that GLSF can improve the therapeutic outcome for colorectal cancer through a mechanism involving suppression of NF-κB-regulated inflammation and carcinogenesis.
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http://dx.doi.org/10.1093/pcmedi/pbab023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982591PMC
December 2021

PD-1 inhibitor induces myocarditis by reducing regulatory T cells, activating inflammatory responses, promoting myocardial apoptosis and autophagy.

Cytokine 2022 Jun 9;157:155932. Epub 2022 Jun 9.

Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

The present study aimed to explore the pathogenesis of autoimmune myocarditis induced by PD-1 inhibitors and their potential therapeutic targets. Mouse models of autoimmune myocarditis induced by PD-1 inhibitor in mouse models of polymyositis were established. The expression level of PD-1 and regulatory T cells (Tregs), CD4, CD8 + T cells, inflammation, apoptosis and autophagy-related factors, including IL-6, TGF-β, AMA-M2, Fas/FasL, LC3 and p62 were detected in peripheral blood, muscle or myocardium of mice in each group, using ELISA, RT-PCR, Western Blot and immunofluorescence. In addition, HE and TUNEL staining and ultrastructural scanning were performed on the myocardium of mice in each group. Results showed that the expression level of PD-1 in the two myositis groups was significantly lower than that in the control group, and the level of PD-1 was lower in the myocarditis group than that in the polymyositis group. In the myocardium, TGF-β, p62, and Tregs proportion showed the same expression level trend as PD-1, while CD8, IL-6, IL-10 and LC3 showed the opposite trend. Levels of Fas/FasL were significantly higher in both myositis groups, but were slightly lower in the myocarditis group, as was AMA-M2. Inflammation, apoptosis, and autophagy were observed in both myositis groups, but were more severe in the myocarditis group. In summary, the decreased expression level of PD-1 leads to decreased Tregs level in the myocardium, aggravated inflammatory response, apoptosis and autophagy, which may be the pathological mechanism of myocarditis induced by PD-1 inhibitors.
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http://dx.doi.org/10.1016/j.cyto.2022.155932DOI Listing
June 2022

A novel KLF11 variant in a family with maturity-onset diabetes of the young.

Pediatr Diabetes 2022 Jun 11. Epub 2022 Jun 11.

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: The Krüppel-like factor 11 (KLF11) gene causes maturity-onset diabetes of the young 7 (MODY7). There are few reports on the clinical and functional characteristics of KLF11 mutations in patients with MODY7, making diagnosis and treatment complicated.

Research Design And Methods: We report a novel KLF11 variant associated with MODY7 in a Chinese family. The proband had hyperglycemia at 9 years of age, and his mother had developed diabetes at age 28 years. Both required insulin injections from the initial phase of the disease. They were negative for islet cell autoantibodies and had normal fasting C-peptide levels. We observed changes in the levels of fasting blood glucose, C-peptide, and islet cell autoantibodies in the proband over 4.5 years.

Results: Whole-exon sequencing was used to screen the proband and his family members for KLF11 variants. The heterozygous KLF11 variant (c.1045C>T, p. Pro349Ser) was identified in the proband, his mother, his maternal grandmother, and an elderly aunt, although the latter two individuals were unaffected. In silico analyses indicated that this variant involved a change in the amino acid side chain in the transcriptional regulatory domain 3. Luciferase reporter assays revealed that the variant had impaired insulin promoter regulation activity. Moreover, in vitro analyses showed that this variant impaired insulin secretion from pancreatic beta cells.

Conclusions: This study documents a novel heterozygous KLF11 variant (p. Pro349Ser) as a potential monogenic mutation associated with MODY7 in a family. This variant impairs insulin secretion from pancreatic beta cells, possibly by repressing insulin promoter regulation activity.
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http://dx.doi.org/10.1111/pedi.13384DOI Listing
June 2022

Research on an Intelligent Identification and Classification Method of Complex Holes in Triangle Meshes for 3D Printing.

Comput Intell Neurosci 2022 31;2022:2249925. Epub 2022 May 31.

Shandong Shanda Hoteam Software Co., Ltd, Jinan, Shandong 250101, China.

In triangular mesh models, the repair of complex hole poses a difficult problem, which always causes serious repair defects. Therefore, it is needed to develop an intelligent identification and classification method of complex holes to reduce repair difficulties. First, the topological structure of the complex hole is studied and all the holes are divided into single holes and continuous holes depending on whether there are intersection points. Second, to tackle the nesting and connecting of complex continuous holes, a decomposition method of multiply connected domains based on intersection points is proposed to partition or reconstruct complex continuous holes into single holes. Based on the different geometric structures, single holes are classified into five common hole types and a corresponding identification method of single holes is presented. Finally, an experiment is carried out to verify the repair quality and efficiency of the proposed method. Compared with Geomagic software, the proposed method can automatically identify and partition complex holes with fewer defects and similar efficiency. It can reduce the difficulties of repairing complex holes and enable the repair of complex holes based on existing methods. It is shown that the method can be applied to complex hole repair of 3D printing models without the participation of technicians.
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http://dx.doi.org/10.1155/2022/2249925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173961PMC
June 2022

Mechanical Modeling of Tube Bending Considering Elastoplastic Evolution of Tube Cross-Section.

Materials (Basel) 2022 Jun 3;15(11). Epub 2022 Jun 3.

School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an 710072, China.

Aluminum alloy tubes are widely used in various industries because of their excellent performance. Up to now, when the tube is bent, the elastoplastic deformation evolution mechanism of the cross-section has not been clear, and no direct analytical proof has been found. In this paper, based on the bilinear material model assumption, a new mechanical model of tube plane bending deformation is constructed. The analytical model can describe in detail the evolution mechanism of elastic-plastic deformation on the cross-section of the tube after bending deformation, the position of the elastic-plastic boundary, the position of the radius of the strain neutral layer, and the relationship between the bending moment over the section and the bending radius. According to this model, the deformation law of the tube cross-section during bending is elucidated. The results are as follows: (1) the deformation evolution of the cross-section of the bending deformed tube calculated by the analytical model is in good agreement with the finite element model (FEM) of pure bending. (2) By comparing the results of the analytical model with FEM results, and the processing test of the self-designed four-axis free bending forming tube bender, the bending moments are in good agreement. (3) Compared with the bending moments calculated by several other analytical models of tube bending, this model has a relatively small deviation value.
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http://dx.doi.org/10.3390/ma15113997DOI Listing
June 2022

[Inhibitory Effect of Interference with PD-L1 Expression on B-cell lymphoma in Mice].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2022 Jun;30(3):771-777

Laboratory of Hematonosis, Northern Jiangsu People's Hospital, Yangzhou Institute of Hematology, Yangzhou 225001, Jiangsu Province, China,E-mail:

Objective: To investigate the potential inhibitory effect of interference with PD-L1 on B cell lymphoma in mice.

Methods: Three shRNA vectors for mouse CD274 (PD-L1) were constructed and transiently transfected into 293T cells. RT-qPCR was used to validate the interference efficiency of CD274. The shRNA vector that interfere efficiently with CD274 expression was packaged by using lentivirus packaging system to generate shRNA lentivirus, and then transfected into A20 lymphoma cell line. The methyl thiazol terazolium (MTT) assay was used to detect proliferation after 48 h culture of CD274-sh A20 cells. Meanwhile, BALB/c mice were hypodermically infected with CD274-sh A20 cells. Infected mice were observed daily and assessed to visualize tumor by in vivo fluorescence imaging.

Results: The proliferation rate of CD274-sh A20 cells in vitro was significantly lower than that of A20 cells (P<0.05). The tumor size detected by in vivo fluorescence imaging showed a significant reduce in tumor bearing mice with CD274-sh compared with other tumor bearing mice. And the weight and size of tumor in CD274-sh group were also significantly reduced compared with other group (P<0.05). Moreover, the survival time of tumor bearing mice in CD274-sh group was longer than that of the PD-L1 high expression group.

Conclusion: PD-L1 plays an important role in the incidence and the progression of lymphoma, and the shRNA-based PD-L1 knockdown can inhibit cell proliferation of A20 cells and partly suppress tumor growth.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.03.018DOI Listing
June 2022

Ginkgo Biloba Extract Ameliorates Scopolamine-induced Memory Deficits via Rescuing Synaptic Damage.

Curr Med Sci 2022 Jun 8;42(3):474-482. Epub 2022 Jun 8.

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Objective: Alzheimer's disease (AD) is the most common age-related neurodegenerative disorder. Emerging evidence suggests that synaptic dysfunction is associated with the onset and progression of AD. Interestingly, Ginkgo biloba extract (EGb) is one of the most frequently investigated herbal medicines for enhancing cognition and alleviating neurodegenerative dementia. This study aimed to investigate the effect and the mechanism of EGb on AD-like synaptic disorders.

Methods: Scopolamine (SCO)-induced rats were used to mimic AD-like memory deficits. Morris water maze test and fear conditioning test were conducted to evaluate the memory status of rats in response to different treatments. Then, the synapse alterations were assessed by Golgi staining, and Western blotting was conducted to assess the protein expression of PSD95, GluN2B, synapsin-1, and synaptophysin. Reverse transcription quantitative polymerase chain reaction was applied to detect the mRNA expression of PSD95 and the levels of miR-1-3p/miR-206-3p.

Results: EGb supplement alleviated the learning and memory deficits induced by SCO in behavioral experiments. Moreover, EGb treatment attenuated synaptic damage elicited by SCO, manifested as increased dendritic spine density and the proportion of mushroom-type spines in hippocampal neurons. Further investigation indicated that EGb rescued the expression of synaptic-related proteins, especially PSD95, and decreased the levels of miR-1-3p/miR-206-3p in the rat hippocampus.

Conclusion: The application of EGb effectively treats SCO-induced memory impairments probably by suppressing miR-1-3p/miR-206-3p and elevating the expression of PSD95.
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http://dx.doi.org/10.1007/s11596-022-2582-8DOI Listing
June 2022

Prediction of major adverse cardiac events is the first critical task in the management of immune checkpoint inhibitor-associated myocarditis.

Cancer Commun (Lond) 2022 Jun 9. Epub 2022 Jun 9.

Department of Respiratory and Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100730, P. R. China.

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http://dx.doi.org/10.1002/cac2.12320DOI Listing
June 2022

Downregulation of lncRNA in the placentas of pregnant women with non‑diabetic fetal macrosomia promotes trophoblast cell proliferation.

Mol Med Rep 2022 08 8;26(2). Epub 2022 Jun 8.

State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China.

Macrosomia is a common perinatal complication, with a series of adverse effects on newborns and pregnant women. However, the effects of long non‑coding RNAs (lncRNAs) on non‑diabetic fetal macrosomia (NDFMS) remain unclear. The aim of the present study was to investigate whether aberrant lncRNA expression in the placenta is involved in the pathogenesis of NDFMS and to elucidate its biological mechanisms. The expression profile of lncRNAs in the placentas of pregnant women with NDFMS was investigated using an Agilent Human LncRNA Microarray. Differentially expressed lncRNAs were selected for validation using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Additionally, the function of lncRNA ubiquitin‑specific peptidase 2 antisense RNA 1 () was investigated using a trophoblast cell line. The results revealed that 763 lncRNAs were upregulated and 129 lncRNAs were downregulated in the placentas of women in the NDFMS group (|FC| ≥2.0). A total of 10 lncRNAs (|FC| ≥4.0, signal value ≥50) were selected for validation using two‑stage RT‑qPCR, indicating that the expression trends of the 10 differentially expressed lncRNAs in the NDFMS group (n=8 vs. 8 and 48 vs. 48) were consistent with the microarray data. In addition, a significant downregulation in the levels of lncRNA was observed in both the microarray data and second‑stage verification. The overexpression of lncRNA induced G1 phase cell cycle arrest and the number of cells entering S phase was reduced. In addition, the viability of HTR‑8/SVneo cells was significantly inhibited when lncRNA was overexpressed. Therefore, these findings demonstrated that lncRNAs were significantly differentially expressed in the placentas of pregnant women with NDFMS and that the downregulation of lncRNA may be involved in the pathogenesis of NDFMS, by promoting trophoblast cell viability.
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http://dx.doi.org/10.3892/mmr.2022.12766DOI Listing
August 2022
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