Publications by authors named "Wei Wei"

4,728 Publications

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[Transcutaneous auricular vagus nerve stimulation promotes gastric motility by up-rgulating α7nAChR and suppressing NF-κB p65 expression in duodenum in rats with functional dyspepsia].

Zhen Ci Yan Jiu 2022 Jun;47(6):517-24

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Objective: To study the effect of transcutaneous auricular vagus nerve stimulation (taVNS) on gastric sensitivity and motility in rats with functional dyspepsia (FD), so as to explore its underlying mechanism in improving FD.

Methods: A total of 48 young SD rats were randomly divided into control (=10), model (=9), taVNS (=9), subdiaphragmatic vagus nerve stimulation (SDVNS, =9) and sham SDVNS (=7) groups. The FD model was established by gavage of 0.1% iodoa-cetamide+2% glucose, once daily for 6 days. Rats in the taVNS group received taVNS (0.5 mA) of optopoint "Heart" and "Stomach" for 30 min, once daily for 14 days, while rats in the SDVNS group received subdiaphragmatic vagus nerve stimulation through the implanted electrode, and those of the sham SDVNS group received only application of the same electrodes without electrical stimulation. Electromyogram (EMG) of the cervical trapezius muscle (reflecting gastric sensitivity) was recorded before and after intragastric expansion via an air ballon and the gastric emptying rate was calculated for assessing the gastric motility. The contents of acetylcholine (ACh), nicotinic acetylcholine receptor α7 subunit (α7nAChR), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the duodenum tissue were detected by enzyme-linked immunosorbent assay (ELISA). The expression of nuclear factor kappa B (NF-κB) p65 in the duodenum tissue was determined by Western blot.

Results: In comparison with the control group, the EMG change rate at intragastric pressure levels of 40, 60 and 80 mm Hg, expression of NF-κB p65 protein, and contents of IL-6, IL-1β and TNF-α were significantly increased (<0.05,<0.01, <0.001), while the gastric emptying rate, ACh and α7nAChR contents considerably decreased (<0.05, <0.001) in the model group. After interventions, the EMG change rate, contents of IL-6, IL-1β and TNF-α, and expression of NF-κB p65 were notably decreased (<0.05, <0.01, <0.001), and the gastric emptying rate, ACh and α7nAChR contents obviously increased (<0.05, <0.001) in both taVNS and SDVNS groups relevant to the model group. In comparison with the sham SDVNS group, the EMG change rate, contents of IL-6, IL-1β and TNF-α, and expression of NF-κB p65 were notably decreased (<0.01, <0.05,<0.001), and the gastric emptying rate, ACh and α7nAChR contents obviously increased (<0.01, <0.001) in the both SDVNS and taVNS groups.

Conclusion: taVNS can reduce gastric sensitivity and promote gastric emptying in FD model rats, which may be closely related to its functions in up-regulating ACh and α7nAChR contents and inhibiting the activation of NF-κB p65 signaling in the duodenum.
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http://dx.doi.org/10.13702/j.1000-0607.20220111DOI Listing
June 2022

Effects of rhythmic auditory stimulation on upper-limb movements in patients with Parkinson's disease.

Parkinsonism Relat Disord 2022 Jun 23;101:27-30. Epub 2022 Jun 23.

Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Introduction: Rhythmic auditory stimulation (RAS) is an effective technique extensively used to alleviate lower-limb bradykinesia in patients with Parkinson's disease (PD). However, RAS effects on upper-limb bradykinesia have not been well studied. This study investigated immediate effects of RAS on upper-limb movements in PD patients and healthy people.

Methods: PD patients (n = 23) and age- and gender-matched healthy controls (n = 23) executed left-hand, right-hand, and both-hand movement tasks of the Purdue Pegboard Test when listening to the beats of RAS, including 100%, 110%, and 120% of the baseline tempo, which was fastest movement performance of each participant without the aid of RAS. Sequence of RAS and tasks was randomized for each participant.

Results: PD patients had slower upper-limb movements than did health controls. An interaction was found between RAS and tasks. In both patients and controls and for all task conditions, 120%RAS induced higher scores than did 110% RAS, and the latter induced higher scores than did 100%RAS. In both patients and controls and for all RAS conditions, the right-hand condition induced higher scores than did the left-hand condition, and the latter induced higher scores than did the both-hand condition.

Conclusions: RAS was effective in regulating upper-limb movements in PD patients, which may be explained by rich neural connections between auditory and motor cortical areas in humans. Clinical practitioners should consider using RAS in clinical therapy. Future neuroimaging studies are needed to explore neural mechanisms of RAS in PD patients.
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http://dx.doi.org/10.1016/j.parkreldis.2022.06.020DOI Listing
June 2022

Metabolome analysis of genus Forsythia related constituents in Forsythia suspensa leaves and fruits using UPLC-ESI-QQQ-MS/MS technique.

PLoS One 2022 28;17(6):e0269915. Epub 2022 Jun 28.

Shijiazhuang Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, Hebei, China.

Forsythia suspensa is a traditional Chinese herb. Its numerous metabolites have important roles, as they possessed a wide range of biological activities. This study explored the accumulations of F. suspensa metabolites by performing widely targeted metabolomic analysis. The metabolites were studied at four stages of fruit development. Metabolites in the fruits and leaves of F. suspensa during fruit development included phenolic acids, flavonoids, lipids, lignans and coumarins, amino acids and their derivatives, terpenes, organic acids, nucleotides and their derivatives, alkaloids, quinones, steroids, and tannins. Fourteen Forsythia related metabolites were detected. Their contents varied among the developmental stages. Statistically significant correlations were found between the levels of forsythoside B and 11-methyl-forsythide, and forsythialan B and phillygenin, in both leaves and fruits. According to the correlation analysis between metabolites, Forsythia related metabolites were divided into two classes and five subclasses. In total, 33 compounds presented significant correlations in both fruits and leaves, which indicated the potential relationship in the synthesis of Forsythia related metabolites. Forsythialan B and phillygenin were both negatively correlated with L-valine, while Z-6,7-epoxyligustilid was positively correlated with both compounds. The quality control compounds forsythiaside A and phillyrin were positively and negatively correlated with uracil, respectively. These metabolomics results may facilitate the biosynthesis of Forsythia related metabolites.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0269915PLOS
June 2022

Comparative transcriptomic analysis reveals the potential mechanism of hot water treatment alleviated-chilling injury in banana fruit.

Food Res Int 2022 Jul 2;157:111296. Epub 2022 May 2.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Banana fruit is prone to chilling injury (CI) during cold storage, resulting in quality deterioration and commodity reduction. The hot water treatment (HWT), dipping banana fruit in hot water (52 °C) for 3 min, reduced CI symptom at 7 °C storage. The purpose of this study was to investigate the potential molecular mechanism of HWT on the alleviation of CI of postharvest banana fruit. It was found that HWT treatment obviously inhibited the increases in CI index, relative electrolytic leakage, and the contents of malonaldehyde (MDA) and O, while enhanced proline accumulation. Further transcriptome analysis in the pericarp of banana fruit was evaluated during storage. The results showed that differentially expressed genes (DEGs) in the comparison between control and HWT group were mainly enriched in photosynthesis, chlorophyll metabolism, lipid metabolism, glutathione metabolism, and brassinosteroid and carotenoid biosynthesis. Moreover, transcriptome expression profiles and RT-qPCR analyses exhibited that the corresponding genes involved in these metabolism pathways and heat shock proteins (HSPs) were upregulated by HWT during cold storage. In general, our findings clearly reveal the potential pathways by which HWT alleviates CI in banana fruit, enriching the theoretical basis for the application of hot water to reduce CI in fruits.
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http://dx.doi.org/10.1016/j.foodres.2022.111296DOI Listing
July 2022

Design, synthesis and biological evaluation of a series of novel pyrrolo[2,3-d]pyrimidin/pyrazolo[3,4-d]pyrimidin-4-amine derivatives as FGFRs-dominant multi-target receptor tyrosine kinase inhibitors for the treatment of gastric cancer.

Bioorg Chem 2022 Jun 14;127:105965. Epub 2022 Jun 14.

Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. Electronic address:

Gastric cancer is the second most lethal cancer across the world. With the progress in therapeutic approaches, the 5-year survival rate of early gastric cancer can reach > 95%. However, the prognosis and survival time of advanced gastric cancer is still somber. Therefore, more effective targeted therapies for gastric cancer treatment are urgently needed. FGFR, VEGFR and other receptor tyrosine kinases have recently been suggested as potential targets for gastric cancer treatment. We herein report the discovery of pyrrolo[2,3-d]pyrimidin/pyrazolo[3,4-d]pyrimidin-4-amine derivatives as a new class of FGFRs-dominant multi-target receptor tyrosine kinase inhibitors. SAR assessment identified the most active compounds 8f and 8k, which showed excellent inhibitory activity against a variety of receptor tyrosine kinases. Moreover, 8f and 8k displayed excellent potency in the SNU-16 gastric cancer cell line. Furthermore, 8f and 8k could inhibit FGFR1 phosphorylation and downstream signaling pathways as well as induce cell apoptosis. In vivo, 8f and 8k suppress tumor growth in the SNU-16 xenograft model without inducing obvious toxicity. These findings raise the possibility that compounds 8f and 8k might serve as potential agents for the treatment of gastric cancer.
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http://dx.doi.org/10.1016/j.bioorg.2022.105965DOI Listing
June 2022

CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients.

Medicine (Baltimore) 2022 Jun 24;101(25):e29347. Epub 2022 Jun 24.

Department of Clinical Laboratory, the Third Central Hospital of Tianjin, Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Artificial Cell Engineering Technology Research Center, Tianjin Institute of Hepatobiliary Disease, Tianjin, China.

Abstract: Patients with nonalcoholic fatty liver disease (NAFLD) have symptoms of a gut microbiota disorder with abnormal amino acid and glycolipid metabolism. This study was designed to analyze the characteristics of gut microbiota in patients with NAFLD, predict the gut microbiota phenotype, explore its role in the diagnosis of NAFLD, and establish its significance in disease progression.The characteristics of the gut microbiota in NAFLD patients (n = 28, 45.8 ± 14.2 years, male/female = 18/10) and healthy subjects (n = 20, 49.6 ± 4.8 years, male/female = 14/6) during March-May 2020 were analyzed using 16S rRNA sequencing technology and the phenotypes with large differences were predicted using the Tax4Fun method. The metabolites in the fecal samples of the patients were analyzed using mass spectrometry, and their correlation with different microorganisms was examined. The accuracy of the gut microbiota in diagnosing NAFLD was investigated by receiver operating characteristic curve analysis.We found that the microbial diversity and Bacteroides/Firmicutes (BF) ratio changed significantly (P < .05) in the feces of NAFLD patients. Phenotypic prediction showed that there were significant differences in the phenotypes of amino acid, glucose, and lipid metabolism of gut microbiota in the NAFLD group (P < .05). receiver operating characteristic curve analysis revealed that combination of Bacteroides and the BF ratio resulted in 88% and 100% sensitivity and specificity, respectively, when used for NAFLD diagnosis. Metabolomics and bioinformatics analysis revealed changes in the metabolism of nicotinate, nicotinamide, and pyrimidine; signaling pathways of calcium and oxytocin; pancreatic secretion with metabolites such as uracil, xanthine, and biliverdin; and enzymes such as xanthine dehydrogenase and xanthine oxidase (P < .05).Therefore, the phenotypic changes may be a potential marker for NAFLD and we considered that a combined analysis of Bacteroides and BF ratio had good diagnostic accuracy for NAFLD.
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http://dx.doi.org/10.1097/MD.0000000000029347DOI Listing
June 2022

Role Medium-Chain Fatty Acids in the Lipid Metabolism of Infants.

Front Nutr 2022 9;9:804880. Epub 2022 Jun 9.

Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, China.

Human breastmilk, the ideal food for healthy infants, naturally contains a high concentration of medium-chain fatty acids (MCFAs, about 15% of total fatty acids). MCFAs are an important energy source for infants due to their unique digestive and metabolic properties. MCFA-enriched oils are widely used in an infant formula, especially the formula produced for preterm infants. Recently, there has been a growing interest in the triglyceride structure of MCFAs in human milk, their metabolism, and their effects on infant health. This study summarized the MCFA composition and structure in both human milk and infant formula. Recent studies on the nutritional effects of MCFAs on infant gut microbiota have been reviewed. Special attention was given to the MCFAs digestion and metabolism in the infants. This paper aims to provide insights into the optimization of formulations to fulfill infant nutritional requirements.
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http://dx.doi.org/10.3389/fnut.2022.804880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218682PMC
June 2022

Ectopic Colonization and Immune Landscapes of Periodontitis Microbiota in Germ-Free Mice With Streptozotocin-Induced Type 1 Diabetes Mellitus.

Front Microbiol 2022 10;13:889415. Epub 2022 Jun 10.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

A two-way relationship between diabetes and periodontitis has been discussed recently. Periodontitis microbiota might affect the immune homeostasis of diabetes, but the molecular mechanism of their interactions is still not clear. The aims of this study were to clarify the possible immune regulatory effects of periodontitis microbiota on diabetes and the correlation between immunomodulation and ectopic colonization. A model of germ-free mice with streptozotocin-induced type 1 diabetes mellitus (T1D), which was orally inoculated with mixed saliva samples for 2 weeks, was used in this study. Those mice were randomly divided into two groups, namely, SP (where the T1D mice were orally inoculated with mixed saliva samples from periodontitis patients) and SH (where the T1D mice were orally inoculated with mixed saliva samples from healthy subjects). Ectopic colonization of saliva microbiota was assessed using culture-dependent method and Sanger sequencing, and the composition of gut microbiota was analyzed using 16S rRNA gene sequencing. Changes in 15 types of immune cells and six cytokines either from the small intestine or spleen were detected by multicolor flow cytometry. The correlation between gut microbiota and immune cells was evaluated by redundancy analysis. Although periodontitis microbiota minorly colonized the lungs, spleens, and blood system, they predominantly colonized the gut, which was mainly invaded by . SH and SP differed in beta diversity of the gut bacterial community. Compared to SH, microbial alteration in small intestine occurred with an increase of , , , , and a decrease of in SP. More types of immune cells were disordered in the spleen than in the small intestine by periodontitis microbiota, mainly with a dramatical increase in the proportion of macrophages, plasmacytoid dendritic cells (pDCs), monocytes, group 3 innate lymphoid cells, CD4-CD8- T cells and Th17 cells, as well as a decline of αβT cells in SP. Cytokines of IFNγ, IL17, and IL22 produced by CD4 + T cells as well as IL22 produced by ILCs of small intestine rose in numbers, and the intestinal and splenic pDCs were positively regulated by gut bacterial community in SP. In conclusion, periodontitis microbiota invasion leads to ectopic colonization of the extra-oral sites and immune cells infiltration, which might cause local or systemic inflammation. Those cells are considered to act as a "bridge" between T1D and periodontitis.
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http://dx.doi.org/10.3389/fmicb.2022.889415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226645PMC
June 2022

The association between lifestyle and COVID-19 vaccine hesitancy in China: A large-scale cross-sectional survey.

J Affect Disord 2022 Jun 23. Epub 2022 Jun 23.

Department of Health management, College of Public Health, Zhengzhou University, Henan, People's Republic of China. Electronic address:

Objective: To assess the association between lifestyle and COVID-19 vaccine hesitancy among Chinese adults and provide recommendations for increasing vaccination rates.

Methods: From August 6, 2021 to August 9, 2021, we recruited 29,925 participants from 31 Chinese provinces via an online questionnaire. We designed a question to assess COVID-19 vaccine hesitancy and used 16 items to assess lifestyle by calculating lifestyle scores. Odds ratios (OR) with 95 % confidence intervals (CI) were used to estimate the association by using binary logistic regression models.

Results: The overall prevalence of COVID-19 vaccine hesitancy was 8.40 % (95 % CI: 8.09-8.72), and the median lifestyle score was 65.00 (interquartile range: 59.00-71.00). After adjusting for potential confounders, the COVID-19 vaccine hesitancy rate decreased significantly with an increase in lifestyle score (P for Trend <0.001). Low COVID-19 vaccine hesitancy rate was associated with lifestyle factors including adequate sleep, never smoking, intermittent drinking, good relationships, working and studying, using hand sanitizer, wearing masks, less gathering activities, and keeping social distance (P < 0.05).

Conclusion: Our findings indicate that high lifestyle score is associated with low vaccine hesitancy rate among Chinese. The government should view the smokers (or the drinkers) as the key to further boosting the vaccination rate. In addition, the publicity and education about wearing masks and keeping social distance should be prioritized.
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http://dx.doi.org/10.1016/j.jad.2022.06.038DOI Listing
June 2022

Taxonomic and metabolic signatures of gut microbiota for assessing the severity of depression and anxiety in major depressive disorder patients.

Neuroscience 2022 Jun 21. Epub 2022 Jun 21.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Major depressive disorder (MDD) is a heterogeneous mental disorder for which the precise assessment of symptom severity remains challenging. Studies have consistently found that the microbiota-gut-brain (MGB) axis is profoundly altered in MDD, but whether MGB-relevant clinical parameters are applicable to depression subphenotyping remains largely unexplored. In this prospective study, we assessed the taxonomic and metabolic signatures of fecal microbiota from 45 unmedicated MDD patients and explored their associations with the severity of depression and anxiety symptoms as measured by HAMD-17 and HAMA-14, respectively. The global microbial compositions of MDD patients with mild, moderate and severe symptoms were largely similar. Nevertheless, multiple discriminative bacterial taxa could be identified among the subgroups across the genus to species level. The abundance of fecal Streptococcus was highly correlated with both HAMD and HAMA scores. Patients with severe depression symptoms showed significantly higher abundance of Phascolarctobacterium and Akkermansia, while enrichment of Akkermansia, Coprococcus and Streptococcus were observed with severe anxiety symptoms. In addition, fecal microbial metabolite indole-3-carboxyaldehyde proved useful to discriminate the severity of depression or anxiety symptoms. Together, our results support the utility of microbial taxa and metabolites as potential MGB-based biomarker panel for stratifying the symptom severity of MDD patients.
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http://dx.doi.org/10.1016/j.neuroscience.2022.06.024DOI Listing
June 2022

A new concept of atomically thin - junction based on CaN/NaN donor-acceptor heterostructure: a first-principles study.

Nanoscale 2022 Jun 24. Epub 2022 Jun 24.

School of Physics, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.

In atomically thin - junctions, traditional strategies such as doping and implantation for realizing a - or -region will fail at the nanoscale, and the Schottky barrier and Fermi level pinning effect taking place in metal-semiconductor contacts seriously suppress the transport properties. In this work, based on first-principles calculations, we propose a new strategy for realizing an ultrathin - junction by vertically stacking nonstoichiometric CaN and NaN monolayers, which represents a kind of donor-acceptor heterostructure with a natural Ohmic contact. It is of great interest to find that the tunneling barrier can be eliminated and the charge transfer quantity is one order of magnitude higher than that between polar monolayers by adjusting the interlayer distance. In addition, at equilibrium the interlayer tunneling can be turned into resonant transport due to the quasi-bonding, thus enabling excellent transmission performance. In accordance with the results, we believe that our new concept of an atomically thin - junction will provide an unprecedented possibility for the development of nanodevices.
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http://dx.doi.org/10.1039/d2nr03072aDOI Listing
June 2022

The role of the LINC01234/miR-433-3p/GRB2 ceRNA network in NSCLC cell malignant proliferation.

Comb Chem High Throughput Screen 2022 Jun 24. Epub 2022 Jun 24.

Department of Cardiothoracic Surgery , Jinling Hospital, zhongshan road 305, China.

Background: Non-small cell lung cancer (NSCLC) is associated with high morbidity and mortality. Dysregulation of lncRNAs leads to NSCLC progression.

Objective: This study aims to explore the regulatory mechanism of lncRNA LINC01234 in NSCLC.

Materials And Methods: LINC01234 expression in NSCLC cells was determined. Cell proliferation was detected using CCK-8, colony formation, and EDU assays after transfection of siRNA LINC01234 into H1299 cells and transfection of pcDNA3.1-LINC01234 into H1975 cells. Subcellular localization of LINC01234 was predicted and the binding relations between LINC01234 and miR-433-3p as well as miR-433-3p and GRB2 were verified. The expression levels of miR-433-3p and GRB2 in NSCLC cells were determined. Joint experiments of miR-433-3p inhibitor + si-LINC01234-1 or oe-GRB2 + si-LINC01234-1 were conducted to verify the role of miR-433-3p and GRB2 in NSCLC cell malignant proliferation.

Results: LINC01234 was abundantly expressed in NSCLC cells. LINC01234 silencing reduced NSCLC cell proliferation while LINC01234 overexpression enhanced cell proliferation. LINC01234 competitively bound to miR-433-3p and miR-433-3p directly targeted GRB2. miR-433-3p knockdown or GRB2 overexpression counteracted the repressive effect of LINC01234 silencing on NSCLC cell malignant proliferation.

Conclusion: LINC01234 competitively bound to miR-433-3p and promoted GRB2 transcription to augment NSCLC cell malignant proliferation.
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http://dx.doi.org/10.2174/1386207325666220624093957DOI Listing
June 2022

Associated risk factor analysis and the prognostic impact of positive resection margins after endoscopic resection in early esophageal squamous cell carcinoma.

Exp Ther Med 2022 Jul 20;24(1):457. Epub 2022 May 20.

Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.

Endoscopic resection for early esophageal cancer has a risk of residual margins. The risk these residual margins pose have not been fully evaluated. The present study aimed to investigate the associated risk factors and prognosis of residual margins following the endoscopic resection of early esophageal squamous cell carcinoma. In total, 369 patients (381 lesions) with early esophageal squamous cell carcinoma treated in the Fourth Hospital of Hebei Medical University (Shijiazhuang, China) with endoscopic resection were retrospectively analyzed. Sex, age, location, tumor diameter, depth of tumor invasion, endoscopic treatment, endoscopic ultrasonography (EUS) before resection, work experience of endoscopists and the degree of tumor differentiation were all evaluated as potential risk factors. In addition, the prognosis of patients with positive margins were analyzed. A total of 73 patients (73/381, 19.2%) had positive margins after endoscopic resection. Amongst the 65 patients who were successfully followed up, five patients succumbed to cardiovascular and cerebrovascular diseases, one patient received radiotherapy, two patients received radiotherapy and chemotherapy whilst one patient received chemotherapy. By contrast, 12 patients received surgery and 20 patients received additional endoscopic mucosal resection or endoscopic submucosal dissection. The other 29 patients were followed up regularly and no recurrence could be found. Univariate analysis revealed that tumor diameter, endoscopic treatment, depth of invasion, EUS before resection, degree of tumor differentiation and direction of invasion were all associated with the positive margin. Multivariate logistic regression analysis then found that EUS before resection, degree of tumor differentiation and depth of tumor invasion are independent risk factors for positive margins after endoscopic resection. These results suggest that poorly differentiated lesions and deeper invasion depth can increase the risk of positive margin after endoscopic resection. As a result, EUS evaluation before resection may reduce the risk of invasion depth. In addition, for poorly differentiated lesions, more aggressive treatment regimens may be recommended for preventing recurrence.
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http://dx.doi.org/10.3892/etm.2022.11384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204577PMC
July 2022

Characterization of Volatile Flavor Compounds in Supercritical Fluid Separated and Identified in Gurum ( Var. ) Seed Oil Using HSME and GC-MS.

Molecules 2022 Jun 17;27(12). Epub 2022 Jun 17.

National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

In this study, the volatile compound profiles of gurum seed oil were determined using two methods: supercritical CO extraction (SFE) and the screw press process (SPP). For volatile compounds extraction and identification, headspace solid-phase micro-extraction (HS-SPME) and GC-MS were used, respectively. A total number of 56 volatile compounds were revealed and identified in oil extracted by SFE, while only 40 compounds were detected in extracted oil by SPP. Acids, aldehydes, esters, ketones, furans, and other components were present in the highest ratio in oil extracted by SFE. In contrast, alcohols and alkenes were found in the highest proportion in oil extracted by SPP. In this study, it was observed that SFE showed an increase in the amounts of volatile compounds and favorably impacted the aroma of gurum seed oil. The results reveal that different extraction methods significantly impact the volatile components of gurum seed oil, and this study can help evaluate the quality of the oil extracted from gurum seeds.
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http://dx.doi.org/10.3390/molecules27123905DOI Listing
June 2022

Nonivamide induces brown fat-like characteristics in porcine subcutaneous adipocytes.

Biochem Biophys Res Commun 2022 Jun 16;619:68-75. Epub 2022 Jun 16.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Obesity, which is associated with type 2 diabetes, is a threat to human health. There are studies, which suggest that some compounds can induce browning of white adipocytes to combat obesity. In this study, we selected nonivamide, an analog of capsaicin, to detect whether it influenced the browning of porcine white adipocytes. First, we found 25 μM nonivamide promoted apoptosis of porcine subcutaneous pre-adipocytes. After pre-adipocytes differentiation, nonivamide inhibited adipogenesis by reducing the expressions of Pparγ, Cebpα, while it promoted lipolysis by up-regulating Hsl, Atgl. Nonivamide also induced browning of porcine subcutaneous adipocytes by up-regulating the expression of brown and beige adipocyte gene markers, such as Prdm16, Cidea, and Slc27a1. Additionally, thermogenesis gene markers Cpt1a and Cpt1b were significantly up-regulated by nonivamide. Furthermore, nonivamide promoted mitochondrial biogenesis by up-regulating the expression of Tfam, Nrf1, Nrf2, and Tomm20. In conclusion, nonivamide is a potent compound to induce porcine adipocyte browning for treating obesity.
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http://dx.doi.org/10.1016/j.bbrc.2022.06.047DOI Listing
June 2022

Vanadium carbide MXene: as a reductant for the synthesis of gold nanoparticles and its biosensing application.

Amino Acids 2022 Jun 22. Epub 2022 Jun 22.

College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.

Vanadium carbide MXene (VC) acts as a new type of two-dimensional (2D) graphene-like transition metal material that has attracted research interest. VC has been widely used in various fields due to its excellent physical and chemical properties. Herein, the self-assembled [email protected] nanoparticles ([email protected]) are prepared by water bath process at 80 °C. With the addition of glutathione (GSH), the absorbance (Abs.) at 550 nm of [email protected] was decreased. Therefore, an optical sensor is developed to detect GSH based on the properties of [email protected] Under the optimal conditions, the detection range is 1-32 µM and the detection limit is 0.099 µM. Furthermore, the proposed GSH sensor exhibits high sensitivity, high selectivity, strong stability, and excellent recovery. The work will expand the application of VC in biosensing.
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http://dx.doi.org/10.1007/s00726-022-03173-1DOI Listing
June 2022

CP-25 enhances OAT1-mediated absorption of methotrexate in synoviocytes of collagen-induced arthritis rats.

Acta Pharmacol Sin 2022 Jun 22. Epub 2022 Jun 22.

Institute of Clinical Pharmacology, Anhui Medical University; Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Centre of Anti-Inflammatory and Immune Medicine, Hefei, 230032, China.

Organic anion transporter 1 (OAT1) plays a major role in mediating the absorption, distribution and excretion of drugs and other xenobiotics in the human body. In this study we explored the OAT1 status in rheumatoid arthritis (RA) patients and arthritic animals and its role in regulating the anti-arthritic activity of methotrexate (MTX). We showed that OAT1 expression was significantly downregulated in synovial tissues from RA patients compared with that in the control patients. In collagen-induced arthritis (CIA) rats, synovial OAT1 expression was significantly decreased compared with the control rats. In synoviocytes isolated from CIA rats, PGE2 (0.003-1.75 μM) dose-dependently downregulated OAT1 expression, resulting in decreased absorption of MTX. Silencing OAT1 in synoviocytes caused a 43.7% reduction in the uptake of MTX. Furthermore, knockdown of OAT1 impaired MTX-induced inhibitory effects on the viability and migration of synoviocytes isolated from CIA rats. Moreover, injection of OAT1-shRNA into articular cavity of CIA rats significantly decreased synovial OAT1 expression and impaired the anti-arthritic action of MTX, while injection of lentivirus containing OAT1 sequences led to the opposite results. Interestingly, we found that paeoniflorin-6'-O-benzene sulfonate (CP-25) upregulated OAT1 expression both in vitro and in vivo and promoted MTX uptake by synoviocytes via regulating OAT1 expression and function. Taken together, OAT1 plays a major role in regulating MTX uptake by synoviocytes and the anti-arthritic activity of MTX. OAT1 is downregulated in RA and CIA rats, which can be improved by CP-25.
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http://dx.doi.org/10.1038/s41401-022-00931-5DOI Listing
June 2022

The role of IL-10 in kidney disease.

Int Immunopharmacol 2022 Jul 3;108:108917. Epub 2022 Jun 3.

Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, PR China. Electronic address:

Kidney disease is a global health problem with high mortality and socioeconomic burden, including acute kidney injury (AKI), chronic kidney disease (CKD), diabetic nephropathy (DN),and lupus nephritis (LN). However, IL-10 is an anti-inflammatory factor. During severe infection in kidney disease, maintaining tissue homeostasis by inhibiting excessive inflammatory response, regulating immune suppression, delaying tissue fibrosis, and promoting tissue repair. Therefore, improving the pathogenesis and identifying effective therapeutic targets are significant for treating and diagnosing kidney diseases. Thus, the current review describes the structure and function of the IL-10 family and focuses on crucial role in kidney disease. We further discuss the regulatory mechanisms through which IL-10 is involved in kidney disease progression.
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http://dx.doi.org/10.1016/j.intimp.2022.108917DOI Listing
July 2022

Normal B cell ranges in infants: A systematic review and meta-analysis.

J Allergy Clin Immunol 2022 Jun 18. Epub 2022 Jun 18.

Department of Neurology and Department of Biomedicine, University Hospital Basel, University of Basel, Basel.

Background: During the first year of life, B cell level is a valuable indicator of whether external factors, such as exposure to B cell depleting therapies, have an adverse impact on immune system development. However, there are no standard reference ranges of B cell levels in healthy infants by age.

Objective: To estimate the normal range of B cell levels in infants, by age, during the first year of life, by pooling data from published studies.

Methods: Studies reporting B cell levels measured using flow cytometry and CD19 markers in healthy infants were identified via a systematic literature review. Quality and feasibility assessments determined suitability for inclusion in meta-analyses by age group and/or continuous age. Means and normal ranges (2.5th-97.5th percentile) were estimated for absolute and percentage B cell levels. Sensitivity analyses assessed the impact of various assumptions.

Results: Of 37 relevant studies identified, 28 were included in at least 1 meta-analysis. Means and normal ranges of B cell levels were found to be 707 (123-2324) cells/μL in cord blood, 508 (132-1369) cells/μL at age 0-1 month, 1493 (416-3877) cells/μL at age 1-6 months and 1474 (416-3805) cells/μL at age >6 months. The continuous age model showed that B cell levels peaked at week 26. Trends were similar for the percentage B cell estimates and in sensitivity analyses.

Conclusion: These meta-analyses provide the first normal reference ranges for B cell levels in infants, by week of age, during the first year of life.
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http://dx.doi.org/10.1016/j.jaci.2022.06.006DOI Listing
June 2022

Microbial and physicochemical responses of anaerobic hydrogen-producing granular sludge to polyethylene micro(nano)plastics.

Water Res 2022 Jun 14;221:118745. Epub 2022 Jun 14.

Center for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia. Electronic address:

Micro(nano)plastics is an emerging contaminant in wastewater that has showed significant impacts on various biological treatment processes. Nevertheless, the underlying effects of micro(nano)plastics with different concentrations and sizes on the anaerobic hydrogen-producing granular sludge (HPG) were still unclear. This work firstly attempted to illustrate the microbial and physicochemical responses of HPG to a shock load of polyethylene microplastics (PE-MPs) with varied concentrations and sizes. The results revealed that the PE-MPs inhibitory effect on hydrogen production by HPG was both concentration- and size-dependent. Specifically, the increase of PE-MPs concentration and the decline of PE-MPs size to nano-sized plastics (NPs) significantly decreased the hydrogen yield, downgraded to 79.9 ± 2.6% and 63.0 ± 3.9% (p = 0.001, and 0.0002) of control, respectively, at higher MPs concentration and the smaller MPs size (i.e., NPs). The higher PE-MPs concentration and PE-NPs also suppressed extracellular polymeric substances (EPS) generation more severely. The critical bio-processes involved in hydrogen production were disturbed by PE-MPs, with the extent of negative impacts depending on the dosage and size of PE-MPs. These adverse impacts further manifested as granule disintegration and loss of cellular activity. Mechanism analysis highlighted the roles of oxidative stress, leachate released from PE-MPs, interaction between PE-NPs and granules inducing physical crushing of HPG that led to possible direct contact between cells and toxic substances.
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http://dx.doi.org/10.1016/j.watres.2022.118745DOI Listing
June 2022

TCR engineered T cells for solid tumor immunotherapy.

Exp Hematol Oncol 2022 Jun 20;11(1):38. Epub 2022 Jun 20.

Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.

T cell immunotherapy remains an attractive approach for cancer immunotherapy. T cell immunotherapy mainly employs chimeric antigen receptor (CAR)- and T cell receptor (TCR)-engineered T cells. CAR-T cell therapy has been an essential breakthrough in treating hematological malignancies. TCR-T cells can recognize antigens expressed both on cell surfaces and in intracellular compartments. Although TCR-T cells have not been approved for clinical application, a number of clinical trials have been performed, particularly for solid tumors. In this article, we summarized current TCR-T cell advances and their potential advantages for solid tumor immunotherapy.
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http://dx.doi.org/10.1186/s40164-022-00291-0DOI Listing
June 2022

CoIn: Correlation Induced Clustering for Cognition of High Dimensional Bioinformatics Data.

IEEE J Biomed Health Inform 2022 Jun 20;PP. Epub 2022 Jun 20.

Analysis of high dimensional biomedical data such as microarray gene expression data and mass spectrometry images, is crucial to provide better medical services including cancer subtyping, protein homology detection,etc. Clustering is a fundamental cognitive task which aims to group unlabeled data into multiple clusters based on their intrinsic similarities. The K-means algorithm is one of the most widely used clustering heuristics that aims at grouping the data objects into meaningful clusters such that the sum of squared Euclidean distances within each cluster is minimized. Its conceptual simplicity and computational efficiency make it easy to be used for wide applications of different data types. However, all features of data in K-means are considered equally in relevance, which distorts the performance when clustering high-dimensional data such as microarray gene expression data, mass spectrometry images, where there exist many redundant variables and correlated variables. In this paper, we propose a new correlation induced clustering, CoIn, to capture complex correlations among high dimensional data and guarantee the correlation consistency within each cluster. We evaluate the proposed method on a high dimensional mass spectrometry dataset of liver cancer tumor to explore the metabolic differences on tissues and discover the intra-tumor heterogeneity (ITH). By comparing the results of baselines and ours, it has been found that our method produces more explainable and understandable results for clinical analysis, which demonstrates the proposed clustering paradigm has the potential with application to knowledge discovery in high dimensional bioinformatics data.
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http://dx.doi.org/10.1109/JBHI.2022.3179265DOI Listing
June 2022

Global trends in anesthetic research over the past decade: a bibliometric analysis.

Ann Transl Med 2022 May;10(10):607

Department of Anesthesia Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China.

Background: Anesthesia is the reversible inhibition of function of the central and/or peripheral nervous system using drugs or other means to ensure a successful operation. This inhibition is mainly manifested as a loss of sensation, especially pain.

Methods: Bibliometric analysis was used to identify the characteristics, hotspots, and frontiers of global anesthesiology scientific output during the past 10 years. Literatures between 2011 and 2020 in the Web of Science Core Collection (WoSCC) were reviewed and analyzed. VOSviewer was used to visualize trends and hotspots in anesthesia research.

Results: A total of 16,213 publications were retrieved and results showed that there was no significant correlation between the number of articles published each year and the year of publication. England had the most published papers, the greatest number of citations (NC), and the highest h-index. The University of London and the British Journal of Anesthesia were the richest affiliate and journal, respectively. The publication written by Heidenreich had the highest global citation score (GCS).

Conclusions: Our research found that global publications on anesthesia have raised. Recently, "surgery", "management", "propofol", and "analgesia" appeared most frequently, which were active areas of research. In the future research, pain management, pediatric anesthesia, safety, dexmedetomidine, will be the hotspot and mainstream trend of research.
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http://dx.doi.org/10.21037/atm-22-1599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201138PMC
May 2022

Multidimensional identification of disaccharide isomers based on non-covalent complexes and tandem mass spectrometry.

Talanta 2022 Jun 13;249:123674. Epub 2022 Jun 13.

Department of Chemistry, Zhejiang University, Hangzhou, 310027, Zhejiang, PR China. Electronic address:

Glycans are the most abundant organic polymers in nature. They are essential to living organisms and regulate a wide range of biological functions. However, mass spectrometry-based identification of glycan isomers remains challenging due to the complexity of their structures including their complex compositions, linkages, and anomeric configurations. In this study, two novel complex ions, the mononuclear copper-bound dimeric ions [(Cu)(A)(L-His)-H] and the mononuclear copper-bound quaternary ions [(Cu)(A)(L-Ser)-H] (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine), were designed for the collision-induced dissociation-based identification and relative quantification of 14 disaccharide isomers. When the unique fragmentation patterns of the above two types of complex ions were mapped into a three-dimensional vector, all the isomers were completely distinguished. Of note, the established method is able to identify mixtures of linkage isomers only using tandem mass spectrometry based on linkage-specific fragment ions of histidine-based complex ions. Finally, the method was successfully applied to the identification and relative quantification of two disaccharide isomers (lactose and sucrose) in dairy beverages. In conclusion, the established method is sensitive to subtle structural differences in disaccharide isomers and has the potential to be used for the differentiation of various glycans.
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http://dx.doi.org/10.1016/j.talanta.2022.123674DOI Listing
June 2022

First case report of complete paternal isodisomy of chromosome 10 harbouring a novel variant in COL17A1 that causes junctional epidermolysis bullosa intermediate.

BMC Med Genomics 2022 Jun 18;15(1):136. Epub 2022 Jun 18.

Center for Reproductive Medicine, Changhai Hospital, Naval Medical University, Shanghai, China.

Background: Uniparental disomy (UPD) is a condition in which both chromosomes are inherited from the same parent, except for imprinting disorders. Uniparental isodisomy (UPiD) may result in a homozygous variant contributing to an autosomal recessive disorder in the offspring of a heterozygous carrier. Junctional epidermolysis bullosa intermediate (JEB intermediate) is an autosomal recessive inherited disease that is associated with a series of gene variants, including those of COL17A1.

Case Presentation: We report the first case of complete paternal UPiD of chromosome 10 harbouring a novel homozygous variant in COL17A1: c.1880(exon23)delG (p.G627Afs*56). This variant led to the clinical phenotype of junctional epidermolysis bullosa intermediate in a 5-year-old child. Trio-whole exome sequencing (Trio-WES) and in silico data analysis were used for variant identification, Sanger sequencing was performed for variant validation, and pathological examination was performed as the gold standard for phenotype confirmation.

Conclusions: We recommend the use of WES as a first-tier test for the diagnosis of epidermolysis bullosa, especially for paediatric patients. Moreover, UPD events should be detected and analysed routinely through WES data in the future.
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http://dx.doi.org/10.1186/s12920-022-01285-xDOI Listing
June 2022

PDGFA-associated protein 1 is a novel target of c-Myc and contributes to colorectal cancer initiation and progression.

Cancer Commun (Lond) 2022 Jun 18. Epub 2022 Jun 18.

National Translational Science Center for Molecular Medicine & Department of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi, 710032, P. R. China.

Background: The mechanism underlying colorectal cancer (CRC) initiation and progression remains elusive, and overall survival is far from satisfactory. Previous studies have shown that PDGFA-associated protein 1 (PDAP1) is upregulated in several cancers including CRC. Here, we aimed to identify the cause and consequence of PDAP1 dysregulation in CRC and evaluate its role as a potential therapeutic target.

Methods: Multi-omics data analysis was performed to identify potential key players in CRC initiation and progression. Immunohistochemistry (IHC) staining was applied to determine the expression pattern of PDAP1 in CRC tissues. Pdap1 conditional knockout mice were used to establish colitis and CRC mouse models. RNA sequencing, a phosphoprotein antibody array, western blotting, histological analysis, 5-bromo-2'-deoxyuridine (BrdU) incorporation assay, and interactome analysis were applied to identify the underlying mechanisms of PDAP1. A human patient-derived xenograft (PDX) model was used to assess the potential of PDAP1 as a therapeutic target.

Results: PDAP1 was identified as a potential key player in CRC development using multi-omics data analysis. PDAP1 was overexpressed in CRC cells and correlated with reduced overall survival. Further investigation showed that PDAP1 was critical for the regulation of cell proliferation, migration, invasion, and metastasis. Significantly, depletion of Pdap1 in intestinal epithelial cells impaired mucosal restitution in dextran sulfate sodium salt-induced colitis and inhibited tumor initiation and growth in colitis-associated cancers. Mechanistic studies showed that c-Myc directly transactivated PDAP1, which contributed to the high PDAP1 expression in CRC cells. PDAP1 interacted with the juxtamembrane domain of epidermal growth factor receptor (EGFR) and facilitated EGFR-mitogen-activated protein kinase (MAPK) signaling activation, which resulted in FOS-related antigen 1 (FRA-1) expression, thereby facilitating CRC progression. Notably, silencing of PDAP1 could hinder the growth of patient-derived xenografts that sustain high PDAP1 levels.

Conclusions: PDAP1 facilitates mucosal restitution and carcinogenesis in colitis-associated cancer. c-Myc-driven upregulation of PDAP1 promotes proliferation, migration, invasion, and metastasis of CRC cells via the EGFR-MAPK-FRA-1 signaling axis. These findings indicated that PDAP1 inhibition is warranted for CRC patients with PDAP1 overexpression.
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http://dx.doi.org/10.1002/cac2.12322DOI Listing
June 2022

Therapeutic potential of MCC950, a specific inhibitor of NLRP3 inflammasome.

Eur J Pharmacol 2022 Jun 14;928:175091. Epub 2022 Jun 14.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Hefei, China. Electronic address:

NOD-like receptor protein 3 (NLRP3), an important intracellular pattern recognition receptor, is a component of the NLRP3 inflammasome along with apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) and pro-caspase-1. Previous studies have shown that dysregulation of NLRP3 inflammasome may be associated with several human diseases, and therefore blocking NLRP3 inflammasome activation may represent a therapeutic strategy for various diseases. MCC950 is a specific small-molecule inhibitor that selectively blocks activation of the NLRP3 inflammasome. In recent years, research on MCC950 has expanded; its targets are gradually being elucidated, and its metabolism and toxicity have been a focus of study. Preclinical research of MCC950 has yielded promising findings, and MCC950 has shown good efficacy in the treatment of autoimmune diseases, cardiovascular diseases, metabolic diseases and other diseases. Furthermore, clinical trials of MCC950 and other inhibitors of NLRP3 inflammasome have also been conducted. In this review, we discuss the drug targets, metabolism, toxicity and preclinical and clinical research advances of MCC950. We further discuss the clinical therapeutic potential of MCC950 to provide insights for the further study and application of MCC950.
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http://dx.doi.org/10.1016/j.ejphar.2022.175091DOI Listing
June 2022

Shugan granule contributes to the improvement of depression-like behaviors in chronic restraint stress-stimulated rats by altering gut microbiota.

CNS Neurosci Ther 2022 Jun 17. Epub 2022 Jun 17.

Department of Neurology, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China.

Aim: The investigation aims to evaluate the potential effect of Shugan Granule (SGKL) on the gut, brain, and behaviors in rats exposed to chronic restraint stress (CRS).

Methods: The fecal microbiota and metabolite changes were studied in rats exposed to CRS and treated with SGKL (0.1 mg/kg/day). Depressive behaviors of these rats were determined through an open-field experiment, forced swimming test, sucrose preference, and weighing. Moreover, LPS-stimulated microglia and CRS-stimulated rats were treated with SGKL to investigate the regulation between SGKL and the PI3K/Akt/pathway, which is inhibited by LY294002, a PI3K inhibitor.

Results: (i) SGKL improved the altered behaviors in CRS-stimulated rats; (ii) SGKL ameliorated the CRS-induced neuronal degeneration and tangled nerve fiber and also contributed to the recovery of intestinal barrier injury in these rats; (iii) SGKL inhibited the hippocampus elevations of TNF-α, IL-1β, and IL-6 in response to CRS modeling; (iv) based on the principal coordinates analysis (PCoA), SGKL altered α-diversity indices and shifted β-diversity in CRS-stimulated rats; (v) at the genus level, SGKL decreased the CRS-enhanced abundance of Bacteroides; (vi) Butyricimonas and Candidatus Arthromitus were enriched in SGKL-treated rats; (vii) altered gut microbiota and metabolites were correlated with behaviors, inflammation, and PI3K/Akt/mTOR pathway; (viii) SGKL increased the LPS-decreased phosphorylation of the PI3K/Akt/mTOR pathway in microglia and inhibited the LPS-induced microglial activation; (ix) PI3K/Akt/mTOR pathway inactivation reversed the SGKL effects in CRS rats.

Conclusion: SGKL targets the PI3K/Akt/mTOR pathway by altering gut microbiota and metabolites, which ameliorates altered behavior and inflammation in the hippocampus.
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http://dx.doi.org/10.1111/cns.13881DOI Listing
June 2022

KANO Model-Enabled Performance Evaluation of Urban Public Sports Services.

Authors:
Yang Liu Wei Wei

Comput Intell Neurosci 2022 7;2022:1749616. Epub 2022 Jun 7.

School of Physical Education and Sports Science, South China Normal University, Guangzhou 510630, China.

Starting from the goal of overall equalization of basic public services by 2035 as proposed in the report of the 19th Party Congress, firstly the research history of the equalization of public sports services in China is analyzed in stages, and a solid theoretical foundation is laid for the construction of the evaluation index system. Secondly, the performance evaluation system of urban public sports services, which is based on KANO model, is constructed by combining the current development status of public sports services in China. Additionally, fuzzy KANO model is used to analyze and categorize the collected indicators and establish a user-oriented evaluation index system. Finally, an empirical study was conducted on a region using the evaluation system, and the results showed that the degree of equalization of public sports services in the region was achieved to a high degree and was basically close to reaching the target requirement of equalization.
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http://dx.doi.org/10.1155/2022/1749616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197651PMC
June 2022

Transcriptomic Profiling of Intracranial Arteries in Adult Patients With Moyamoya Disease Reveals Novel Insights Into Its Pathogenesis.

Front Mol Neurosci 2022 31;15:881954. Epub 2022 May 31.

Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan, China.

Moyamoya disease (MMD) is a rare, progressively steno-occlusive cerebrovascular disorder of unknown etiology. Here, we revealed the gene expression profile of the intracranial arteries in MMD the RNA-sequencing (RNA-seq). We identified 556 differentially expressed genes (DEGs) for MMD, including 449 and 107 significantly upregulated or downregulated genes. Compared with atherosclerosis-associated intracranial artery stenosis/occlusion (AS-ICASO) controls, upregulated genes were mainly involved in extracellular matrix (ECM) organization, whereas downregulated genes were primarily associated with mitochondrial function and oxidative phosphorylation in MMD. Moreover, we found that a separate sex analysis uncovers more DEGs ( = 1.022) compared to an combined sex analysis in MMD. We identified 133 and 439 sex-specific DEGs for men and women in MMD, respectively. About 95.6% of sex-specific DEGs were protein-coding genes and 3% of the genes belonged to long non-coding RNAs (lncRNA). Sex-specific DEGs were observed on all chromosomes, of which 95.49 and 96.59% were autosomal genes in men and women, respectively. These sex-specific DEGs, such as aquaporin-4 (AQP4), superoxide dismutase 3 (SOD3), and nuclear receptor subfamily 4 group A member 1 (NR4A1), may contribute to sex differences in MMD. This transcriptomic study highlighted that ECM and mitochondrial function are the central molecular mechanisms underlying MMD, and revealed sex differences in the gene expression in the intracranial arteries, thereby providing new insights into the pathogenesis of MMD.
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http://dx.doi.org/10.3389/fnmol.2022.881954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197469PMC
May 2022
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