Publications by authors named "Wei Tang"

1,864 Publications

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A Vision-Based System for Stage Classification of Parkinsonian Gait Using Machine Learning and Synthetic Data.

Sensors (Basel) 2022 Jun 13;22(12). Epub 2022 Jun 13.

Klipsch School of Electrical Engineering, New Mexico State University, Las Cruces, NM 88003, USA.

Parkinson's disease is characterized by abnormal gait, which worsens as the condition progresses. Although several methods have been able to classify this feature through pose-estimation algorithms and machine-learning classifiers, few studies have been able to analyze its progression to perform stage classification of the disease. Moreover, despite the increasing popularity of these systems for gait analysis, the amount of available gait-related data can often be limited, thereby, hindering the progress of the implementation of this technology in the medical field. As such, creating a quantitative prognosis method that can identify the severity levels of a Parkinsonian gait with little data could help facilitate the study of the Parkinsonian gait for rehabilitation. In this contribution, we propose a vision-based system to analyze the Parkinsonian gait at various stages using linear interpolation of Parkinsonian gait models. We present a comparison between the performance of a k-nearest neighbors algorithm (KNN), support-vector machine (SVM) and gradient boosting (GB) algorithms in classifying well-established gait features. Our results show that the proposed system achieved 96-99% accuracy in evaluating the prognosis of Parkinsonian gaits.
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http://dx.doi.org/10.3390/s22124463DOI Listing
June 2022

A Novel Bi-Functional Fibrinolytic Enzyme with Anticoagulant and Thrombolytic Activities from a Marine-Derived Fungus ZLH-1.

Mar Drugs 2022 May 27;20(6). Epub 2022 May 27.

School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

Fibrinolytic enzymes are important components in the treatment of thrombosis-associated disorders. A new bi-functional fibrinolytic enzyme, versiase, was identified from a marine-derived fungus ZLH-1. The enzyme was isolated from the fungal culture through precipitation with ammonium sulfate at 90% saturation. Additionally, it was further purified by DEAE-based ion-exchange chromatography, with a recovery of 20.4%. The fibrinolytic enzyme presented as one band on both SDS-PAGE and fibrin-zymogram, with a molecular mass of 37.3 kDa. It was elucidated as a member of metalloprotease in M35 family by proteomic approaches. The homology-modeling analysis revealed that versiase shares significant structural homology wuth the zinc metalloendopeptidase. The enzyme displayed maximum activity at 40 °C and pH 5.0. The activity of versiase was strongly inhibited by the metalloprotease inhibitors EDTA and BGTA. Furthermore, versiase hydrolyzed fibrin directly and indirectly via the activation of plasminogen, and it was able to hydrolyze the three chains (α, β, γ) of fibrin(ogen). Additionally, versiase demonstrated promising thrombolytic and anticoagulant activities, without many side-effects noticed. In conclusion, versiase appears to be a potent fibrinolytic enzyme deserving further investigation.
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http://dx.doi.org/10.3390/md20060356DOI Listing
May 2022

A comparison of Chinese multicenter breast cancer database and SEER database.

Sci Rep 2022 Jun 21;12(1):10395. Epub 2022 Jun 21.

Department of Breast Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, Yunnan, People's Republic of China.

There are different characteristics of BC in developing countries and developed countries. We intended to study the factors which influence the survival and prognosis of BC between southern China and the United States. (a) To study the two groups BC patients in southern China from 2001 to 2016 and SEER database from 1975 to 2016. (b) To register, collect and analyze the clinicopathological features and treatment information. Our study found that there are significant differences in tumor size, positive lymph node status and KI-67 between southern China and SEER cohort (P < 0.000). The positive lymph node status may be one of the causes of difference of morbidity and mortality of BC patients in China. Furthermore, the differences in treatment methods may also account for the differences between China and seer databases.
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http://dx.doi.org/10.1038/s41598-022-14573-4DOI Listing
June 2022

JAML promotes acute kidney injury mainly through a macrophage-dependent mechanism.

JCI Insight 2022 Jun 16. Epub 2022 Jun 16.

Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, China.

Although macrophages are undoubtedly attractive therapeutic targets for acute kidney injury (AKI) because of their critical roles in renal inflammation and repair, the underlying mechanisms of macrophage phenotype switching and efferocytosis in the regulation of inflammatory responses during AKI are still largely unclear. The present study was to elucidate the role of JAML (junctional adhesion molecule-like protein) in the pathogenesis of AKI. We found that JAML was significantly up-regulated in the kidney from two different murine AKI models including renal ischemia/reperfusion injury (IRI) and cisplatin-induced AKI. By generation of bone marrow chimeric mice, macrophage-specific and tubular-specific Jaml conditional knockout mice, we demonstrated JAML promotes AKI mainly via a macrophage-dependent mechanism and found that JAML-mediated macrophage phenotype polarization and efferocytosis is one of critical signal transduction pathways linking inflammatory responses to AKI. Mechanistically, the effects of JAML on the regulation of macrophages was at least in part, associated with a Mincle-dependent mechanism. Collectively, our studies for the first time explore new biological functions of JAML in macrophages and conclude that JAML is an important mediator and biomarker of AKI. Pharmacologic targeting of JAML mediated signaling pathways at multiple levels may provide a novel therapeutic strategy for patients with AKI.
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http://dx.doi.org/10.1172/jci.insight.158571DOI Listing
June 2022

Norfloxacin adsorption and subsequent degradation on ball-milling tailored N-doped biochar.

Chemosphere 2022 Sep 8;303(Pt 3):135264. Epub 2022 Jun 8.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing, 100083, PR China; School of Earth Sciences and Resources, China University of Geosciences, Beijing, 100083, PR China. Electronic address:

N-doping is an effective way to modify biochar for enhancing the adsorption capacity. The synthesis of N-doped biochar by the ball-milling method has been attractive due to its facile and eco-friendly approach with low energy consumption. However, the commonly used N-precursor NH·HO is environmentally harmful. It is needed to prepare safe and non-toxic N-doped biochar for large-scale production. Here, a urea N-doped biochar (U-MBC) was prepared by the ball-milling method and used for norfloxacin (NOR) removal. The results showed that U-MBC exhibited almost 4-fold higher adsorption capacity for NOR than pristine biochar in a wide pH range (3-9). The adsorption enhancement was owing to the enhancement of H-bonds, π-π electron donor-acceptor, and pore-filling interactions due to the N-doping and ball-milling method. Additionally, 89% of adsorbed NOR can be further removed after 6 h milling. The regenerated U-MBC still had a good adsorption capacity (46.27 mg g) and performed well in three cycles. The knowledge gained from this study could encourage researchers to use urea or similar safe N-precursors with the ball-milling method for the large-scale production of N-doped biochar to remove antibiotic organic pollutants in the environment.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135264DOI Listing
September 2022

Elevated peripheral Neuregulin-1 protein levels in non-medicated focal epilepsy patients.

J Clin Neurosci 2022 Jun 7;102:1-4. Epub 2022 Jun 7.

Department of Science and Education, First Affiliated Hospital of Kunming Medical University, Kunming, China. Electronic address:

Introduction: The Neuregulin-1 (NRG-1) gene has been identified as a susceptibility gene for schizophrenia. Schizophrenia and epilepsy shared some common clinical manifestations and common pathogenesis. Therefore, it is necessary to explore whether there is a relationship between NRG-1 and epilepsy. This study aimed to investigate the expression level of NRG-1 in peripheral blood of non-medicated patients with first-onset focal epilepsy.

Methods: A total of 83 non-medicated first-onset focal epilepsy patients and 80 healthy controls were involved in this study. Serum NRG-1 protein levels were determined by ELISA.

Results: Compared to healthy controls (mean ± SD, 3.97 ± 2.37), NRG-1 protein levels were statistically significantly higher in patients (mean ± SD, 5.37 ± 3.48) (P = 0.006).

Conclusions: Our findings suggest that NRG-1 protein may play a role in the pathogenesis of focal epilepsy, which provides insights into the search for epilepsy potential therapeutic markers and new drug treatment targets.
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http://dx.doi.org/10.1016/j.jocn.2022.06.001DOI Listing
June 2022

2D Gait Skeleton Data Normalization for Quantitative Assessment of Movement Disorders from Freehand Single Camera Video Recordings.

Sensors (Basel) 2022 Jun 2;22(11). Epub 2022 Jun 2.

Department of Neurology, University Medical Center Groningen, University of Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.

Overlapping phenotypic features between Early Onset Ataxia (EOA) and Developmental Coordination Disorder (DCD) can complicate the clinical distinction of these disorders. Clinical rating scales are a common way to quantify movement disorders but in children these scales also rely on the observer's assessment and interpretation. Despite the introduction of inertial measurement units for objective and more precise evaluation, special hardware is still required, restricting their widespread application. Gait video recordings of movement disorder patients are frequently captured in routine clinical settings, but there is presently no suitable quantitative analysis method for these recordings. Owing to advancements in computer vision technology, deep learning pose estimation techniques may soon be ready for convenient and low-cost clinical usage. This study presents a framework based on 2D video recording in the coronal plane and pose estimation for the quantitative assessment of gait in movement disorders. To allow the calculation of distance-based features, seven different methods to normalize 2D skeleton keypoint data derived from pose estimation using deep neural networks applied to freehand video recording of gait were evaluated. In our experiments, 15 children (five EOA, five DCD and five healthy controls) were asked to walk naturally while being videotaped by a single camera in 1280 × 720 resolution at 25 frames per second. The high likelihood of the prediction of keypoint locations (mean = 0.889, standard deviation = 0.02) demonstrates the potential for distance-based features derived from routine video recordings to assist in the clinical evaluation of movement in EOA and DCD. By comparison of mean absolute angle error and mean variance of distance, the normalization methods using the Euclidean (2D) distance of left shoulder and right hip, or the average distance from left shoulder to right hip and from right shoulder to left hip were found to better perform for deriving distance-based features and further quantitative assessment of movement disorders.
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http://dx.doi.org/10.3390/s22114245DOI Listing
June 2022

The Impact of Telemedicine on Rheumatology Care.

Front Med (Lausanne) 2022 20;9:876835. Epub 2022 May 20.

Division of Rheumatology, Columbia University Irving Medical Center, New York, NY, United States.

Background: The pandemic disrupted the care of patients with rheumatic diseases; difficulties in access to care and its psychological impact affected quality of life. Telemedicine as an alternative to traditional face-to-face office visits has the potential to mitigate this impact.

Objective: To evaluate patient and provider experience with telemedicine and its effect on care.

Methods: We surveyed patients with rheumatic diseases and their rheumatology providers. The surveys were conducted in 2020 and repeated in 2021. We assessed data on quality of care and health-related quality of life.

Results: Hundred patients and 17 providers responded to the survey. Patients reported higher satisfaction with telemedicine in 2021 compared to 2020 (94 vs. 84%), felt more comfortable with (96 vs. 86%), expressed a stronger preference for (22 vs. 16%), and higher intention to use telemedicine in the future (83 vs. 77%); patients thought physicians were able to address their concerns. While providers' satisfaction with telemedicine increased (18-76%), 14/17 providers believed that telemedicine visits were worse than in-person visits. There were no differences in annualized office visits and admissions. Mean EQ-5D score was 0.74, lower than general population (0.87) but equivalent to a subset of patients with SLE (0.74).

Conclusion: Our data showed a high level of satisfaction with telemedicine. The lower rheumatology provider satisfaction raises concern if telemedicine constitutes an acceptable alternative to in-person care. The stable number of office visits, admissions, and the similar quality of life to pre-pandemic level suggest effective management of rheumatic diseases using telemedicine/in-person hybrid care.
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http://dx.doi.org/10.3389/fmed.2022.876835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163385PMC
May 2022

Mild Hypothermia Promotes Ischemic Tolerance and Survival of Neural Stem Cell Grafts by Enhancing Global SUMOylation.

Oxid Med Cell Longev 2022 27;2022:6503504. Epub 2022 May 27.

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.

Cerebral infarct penumbra due to hypoxia and toxin accumulation is not conducive to the transplantation of neural stem cells (NSCs), although mild hypothermia can improve the local microenvironment of the ischemic penumbra and exert neuroprotective effects. However, insufficient understanding of the molecular mechanism by which mild hypothermia protects the brain limits widespread clinical application. This study evaluated the molecular mechanism of mild hypothermia-induced brain protection from the perspective of global protein small ubiquitin-like modifier (SUMO) modification, with the aim of improving NSC transplant survival rates in the penumbra to enhance neurological function. NSCs from neonatal rats were extracted to detect the effects of hypoxia and mild hypothermia on SUMOylation modification levels, cell stemness, and hypoxia-induced injury. Overexpression and knockdown of UBC9 in NSCs were used to evaluate their ability to maintain stemness and withstand hypoxic injury. Finally, a rat middle cerebral artery occlusion (MCAO) model was used to verify the effect of mild hypothermia treatment and UBC9 overexpression on neural function of NSCs following penumbra transplantation in rats. Results showed that hypoxia and mild hypothermia promoted both the SUMOylation modification and maintenance of NSC stemness. Overexpression of UBC9 enhanced the abilities of NSCs to maintain stemness and resist hypoxic injury, while UBC9 knockdown had the opposite effect. Following transplantation into the ischemic penumbra of MCAO model rats, mild hypothermia and -overexpressing NSCs significantly reduced cerebral infarct areas and improved neurological function. In conclusion, this study demonstrated that global protein SUMOylation is an important molecular mechanism for NSCs to tolerate hypoxia, and mild hypothermia can further increase the degree of global SUMOylation to enhance the hypoxia tolerance of NSCs, which increases their survival during transplantation in situ and ability to perform nerve repair in the penumbra of cerebral infarction.
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http://dx.doi.org/10.1155/2022/6503504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166982PMC
June 2022

Stilbenes from the leaves of Cajanus cajan and their in vitro anti-inflammatory activities.

Fitoterapia 2022 Jul 2;160:105229. Epub 2022 Jun 2.

Center for Bioactive Natural Molecules and Innovative Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address:

Eighteen stilbenes (1-18), including six previously undescribed ones (1-6), with diverse modification patterns were isolated from the leaves of edible and medicinal plant Cajanus cajan. Among the new isolates, compounds 1-3 were initially obtained as three racemic mixtures, which were further resolved into three pairs of optically pure enantiomers, respectively, by chiral HPLC. Besides, compounds 8, 10, 11, and 18 were obtained from C. cajan for the first time. The chemical structures and absolute configurations of the new stilbenes were elucidated unambiguously on the basis of extensive spectroscopic analyses, single crystal X-ray crystallographic study, and quantum chemical electronic circular dichroism (ECD) calculations. In addition, the in vitro anti-inflammatory activities of all isolated stilbenes were evaluated. Compounds 2, 9, 10, 11, and 14 exerted moderate suppression of nitric oxide (NO) secretion in lipopolysaccharide (LPS)-induced RAW264.7 cells without exhibiting substantial cytotoxicity.
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http://dx.doi.org/10.1016/j.fitote.2022.105229DOI Listing
July 2022

Pretreatment Multiparametric MRI-Based Radiomics Analysis for the Diagnosis of Breast Phyllodes Tumors.

J Magn Reson Imaging 2022 Jun 3. Epub 2022 Jun 3.

Department of Radiology, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: Preoperative pathological grading assessment is important for patients with breast phyllodes tumors (PTs).

Purpose: To develop and validate a clinical-radiomics model based on multiparametric MRI and clinical information for the pretreatment differential diagnosis of PTs.

Study Type: Retrospective.

Population: A total of 216 patients with PTs, 133 in the training cohort (55 benign PTs [BPTs] and 78 borderline/malignant PTs [BMPTs]) and 83 in the validation cohort (28 BPTs and 55 BMPTs).

Field Strength/sequence: 1.5 T and 3 T; T2-weighted imaging (T2WI), precontrast T1-weighted imaging (T1WI) and dynamic contrast-enhanced T1-weighted imaging (DCE-T1WI).

Assessment: A total of 3138 radiomics features were computed to decode the imaging phenotypes of PTs. To build the classification models, the following workflow was followed: minimum-maximum scaling normalization method, recursive feature elimination based on ridge regression (Ridge-RFE), synthetic minority oversampling technique, and support vector machine classifier. We established several models based on the statistically significant features (Ridge-RFE selected) of each sequence to distinguish BPTs from BMPTs, including precontrast T1WI model, DCE-T1WI phase 1 model, T1WI feature fusion model, T2WI model, T1WI + T2WI model, clinical feature model, conventional MRI characteristics model, and combined clinical-radiomics model.

Statistical Tests: Univariate analysis was utilized to compare variables between the BPT and BMPT groups. The receiver operating characteristic curve (ROC) analysis was used to evaluate the diagnostic performance of these models.

Results: In the training cohort, the clinical-radiomics model had excellent diagnostic efficiency, with an area under ROC (AUC) of 0.91 ± 0.02 (95% CI: 0.87-0.94). In the validation cohort, the AUCs were 0.79 ± 0.05 (95% CI: 0.70-0.87) for the combined model and 0.77 ± 0.05 (95% CI: 0.67-0.85) for the radiomics model.

Data Conclusion: Compared with conventional MRI characteristics, radiomics features extracted from multiparametric MRI are helpful for improving the accuracy of differentiating the pathological grades of PTs preoperatively. The model based on radiomics and clinical information is expected to become a potential noninvasive tool for the assessment of PTs grades.

Evidence Level: 4 TECHNICAL EFFICACY: Stage 2.
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http://dx.doi.org/10.1002/jmri.28286DOI Listing
June 2022

The Clinical Study of Bladder Flap Ureteroplasty (Psoas Hitch) in the Treatment of Lower Ureteral Injuries and Strictures (19 Cases).

Evid Based Complement Alternat Med 2022 24;2022:4607735. Epub 2022 May 24.

Department of Urology, Hebei Yanda Hospital, Langfang City 065201, Hebei Province, China.

Objective: The aim of this study was to investigate the efficacy and safety of bladder flap ureteroplasty (psoas hitch) in the treatment of lower ureteral injuries and strictures.

Methods: 19 patients with lower ureteral injuries and strictures scheduled for a bladder flap ureteroplasty (psoas hitch) in our hospital from January 2020 to January 2021 were recruited. The outcome measures included treatment efficacy and safety.

Results: The operative time, intraoperative bleeding, catheter extubation time, hospital stay, extubation time of ureteral stent, and follow-up time were (125.36 ± 15.38) min, (75.37 ± 11.09) ml, (7.25 ± 1.04) d, (8.76 ± 1.11) d, (46.34 ± 7.66) d, and(19.27 ± 1.27) months, respectively. No serious perioperative adverse reactions were observed, and all the symptoms of patients were relieved.

Conclusion: Bladder flap ureteroplasty (psoas hitch) is safe and effective for the treatment of lower ureteral injuries, with advantages such as less intraoperative bleeding and trauma and rapid recovery, so it is worthy of promotion. This was a retrospective study supervised by the Ethics Committee of Hebei Yanda Hospital.This trial is registered with no. hebYD076.
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http://dx.doi.org/10.1155/2022/4607735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9155903PMC
May 2022

Hemin protects against Zika virus infection by disrupting virus-endosome fusion.

Antiviral Res 2022 Jul 26;203:105347. Epub 2022 May 26.

School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210023, PR China; Laboratory of Anti-inflammation and Immunopharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China. Electronic address:

Zika virus (ZIKV) is a flavivirus that causes severe neuropathology in newborns and adults. There is no ZIKV-specific treatment or preventative. Therefore, it is urgent to develop safe and effective anti-ZIKV agents. Hemin, an iron-binding porphyrin, has been authorized by FDA to treat acute porphyria since the 1970s. Here, we aim to evaluate the anti-ZIKV effect of hemin in SNB-19 cells (a human glioma cell line) and explore the underlying mechanism based on the virus life cycle and functions of the host cell. Our study found that hemin has a strong activity to protect SNB-19 cells from ZIKV infection presented by decreased expression of viral proteins and virus yield. Meanwhile, ZIKV infection caused STAT1/IRF1 signaling activation and induced inflammatory responses in SNB-19 cells, which was relieved by hemin treatment. HO-1 has been reported to be potently induced by hemin and play a broad-spectrum antiviral effect. Intriguingly, hemin could still exert anti-ZIKV activity upon HO-1 siRNA treatment. Then, we conducted a time-of-addition assay, the result indicated hemin works mainly by interfering with the virus entry process. Further experiments excluded the effects of hemin on AXL-dependent viral adsorption and clathrin-mediated endocytosis processes. Subsequently, by fluorescence spectroscopy studies, intracellular fusion assay and syncytia formation assay, we revealed that hemin acts on the process of virus-endosome fusion. This study elaborated that hemin could play anti-ZIKV activity by disrupting the virus-endosome fusion process and shed new light on developing novel agents against ZIKV infection.
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http://dx.doi.org/10.1016/j.antiviral.2022.105347DOI Listing
July 2022

Electrochemical exfoliation of two-dimensional phosphorene sheets and its Energy application.

Chemistry 2022 May 31. Epub 2022 May 31.

Xi'an Jiaotong University, State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, CHINA.

Recently, single or few-layer phosphorene has aroused intense attention due to its exceptional physicochemical properties. To this end, mass production of high-quality phosphorene nanosheets with specific functionalities represents a pivotal factor for the basic academic studies and practical applications. Among the current synthetic methods, electrochemical exfoliation of black phosphorous is one of the most hopeful ways for mass-production of phosphorene sheets owing to the uncomplicated apparatus, low cost as well as significant efficiency. Especially, regulating the electrochemical parameters not only induces adjustable phosphorene characteristics but also enables them a promising candidate in energy applications. In this review, a concise and crucial studies of the recent and most representative developments in this domain was introduced, including the relationship between exfoliation philosophy, internal mechanisms, processing techniques, and multiple applications of phosphorene. At the end, a summary discussion and future perspectives is also provided.
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http://dx.doi.org/10.1002/chem.202200857DOI Listing
May 2022

Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.

Kidney Int 2022 May 25. Epub 2022 May 25.

Key Laboratory of Infection and Immunity of Shandong Province, Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, China. Electronic address:

Podocytes are unique, highly specialized, terminally differentiated cells, which are restricted in a post-mitotic state with limited ability to repair or regenerate. Re-entering the mitotic phase causes podocyte mitotic catastrophe, thereby leading to podocyte death and glomerular injury. Myeloid-derived growth factor (MYDGF) is a novel secreted protein and plays an important role in the regulation of cardiovascular function. However, whether MYDGF is expressed in kidney parenchymal cells and whether it has biological functions in the kidney remain unknown. Here, we found that MYDGF was expressed in kidney parenchymal cells and was significantly reduced in podocytes from mice with models of focal segmental glomerulosclerosis and diabetic kidney disease. Podocyte-specific deletion of MYDGF in mice exacerbated podocyte injury and proteinuria in both disease models. Functionally, MYDGF protected podocytes against mitotic catastrophe by reducing accumulation of podocytes in the S phase, a portion of the cell cycle in which DNA is replicated. Mechanistically, MYDGF regulates the expression of the transcription factor RUNX2 which mediates some MYDGF effects. Importantly, a significant reduction of MYDGF was found in glomeruli from patients with glomerular disease due to focal segmental glomerulosclerosis and diabetic kidney disease and the level of MYDGF was correlated with glomerular filtration rate, serum creatinine and podocyte loss. Thus, our studies indicate that MYDGF may be an attractive therapeutic target for glomerular disease.
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http://dx.doi.org/10.1016/j.kint.2022.04.027DOI Listing
May 2022

Diacylglycerol kinase epsilon protects against renal ischemia/reperfusion injury in mice through Krüppel-like factor 15/klotho pathway.

Ren Fail 2022 Dec;44(1):902-913

Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, China.

Although recent studies have indicated that mutations in the gene encoding diacylglycerol kinase epsilon (DGKE) result in some proteinuria related hereditary kidney diseases, the DGKE expression pattern in the kidney and its contribution to acute kidney injury (AKI) remain unknown. Therefore, the present study was designed to detect the role of DGKE in mice with AKI. DGKE expression was time-dependently altered in the kidneys of mice with renal ischemia/reperfusion injury (IRI). Compared with wild-type (WT) mice, DGKE- overexpressing mice () exhibited protective effects against renal IRI, including reduced serum creatinine, blood urea concentration, tubular cell death and inflammatory responses as well as improved morphological injuries. Consistently, , DGKE overexpression in human renal proximal tubule (HK-2) cells also protected against oxygen-glucose deprivation (OGD)/reoxygenation-induced cell death. Mechanistically, DGKE regulated Klotho expression, at least partly the transcription factor Krüppel-like factor (KLF) 15. Moreover, a significant reduction in DGKE was also found in kidneys from patients with ischemia-associated acute tubular necrosis (ATN). Collectively, our studies demonstrate that DGKE protects against AKI in mice at least partly through KLF15/Klotho signaling pathway, indicating that DGKE may present an innovative therapeutic strategy for treating patients with AKI.
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http://dx.doi.org/10.1080/0886022X.2022.2079524DOI Listing
December 2022

Genomic characterization and expression analysis of transcription factors in and .

Plant Signal Behav 2022 12;17(1):2075158

Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, Shandong, China.

The plant-specific transcription factor plays important roles in plant development and environment adaptation. and , the C4 model plants, can grow on drought or arid soils. However, there is no systematic information about the genomic dissection and the expression of genes. A total of 22 genes were both identified from and genomes. They all contained bHLH domain and were grouped into three main clades (PCF, CIN, and CYC/TB1). The genes in the same clades shared similar gene structures. Cis-element in the promoter regions were analyzed and associated with hormones and stress responsiveness. Ten genes were predicted to be targets of miRNA319. Moreover, gene ontology analysis indicated three Si and three genes were involved in the regulation of shoot development, and were clustered together with tillering controlling gene . The genes were differentially expressed in the organs, but orthologs shared similar expression patterns. Ten members were downregulated under drought or salinity stresses, indicating they may play regulatory roles in abiotic stresses. The study provides detailed information regarding genes, providing the theoretical basis for agricultural applications.
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http://dx.doi.org/10.1080/15592324.2022.2075158DOI Listing
December 2022

Role of IL-25 on Eosinophils in the Initiation of Th2 Responses in Allergic Asthma.

Front Immunol 2022 9;13:842500. Epub 2022 May 9.

Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Eosinophils act as a secondary antigen-presenting cell (APC) to stimulate Th cell responses against antigens. IL-25 plays a significant role in eosinophil activation in allergic asthma. The role of IL-25 on the classic APC functions of dendritic cells has been elucidated. However, whether IL-25 facilitates eosinophils for antigen presentation is unknown.

Objective: To elucidate the role of IL-25 on eosinophils antigen presenting function during allergic asthma and its related mechanism.

Methods: Eosinophils from allergic asthma subjects were cultured with IL-25 and HDM to identify the co-stimulator molecules expression. Co-cultures of patient eosinophils and autologous naïve CD4 T cells in the same culture system were to explore whether eosinophils had the capacity to promote Th cell differentiation in response to IL-25 engagement. In asthma mouse model, IL-25 mice were exposed to HDM to investigate the effect of IL-25 on eosinophils during the sensitization phase. The impact of IL-25 on the capacity for eosinophils taking up antigens was evaluated. Mouse bone marrow derived eosinophils (BmEOS) were co-cultured with naïve CD4T cells sorted from spleens under HDM and IL-25 stimulation to identify T cell differentiation.

Results: IL-25 upregulated HLA-DR, PD-L1, and OX-40L expression on eosinophils from allergic asthma patients. IL-25 and HDM co-sensitized eosinophils promoted Th2 differentiation. In mouse model, IL-25 mice experienced restrained allergic pulmonary inflammation and reduced eosinophils recruitment and antigen uptake capacity during the early sensitization phase. , IL-25 promoted antigen uptake by eosinophils. In BmEOS and naïve CD4T cells co-culture, IL-25 accreted the proportion of CD4Th2 cells, which was absent in CD4T cells culture alone.

Conclusion: Our data identify a novel role of IL-25 in enhancing eosinophils antigen uptake and co-stimulator molecules expression to induce Th2 priming in the context of allergic inflammation.
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http://dx.doi.org/10.3389/fimmu.2022.842500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125245PMC
May 2022

Multi-class retinal fluid joint segmentation based on cascaded convolutional neural networks.

Phys Med Biol 2022 06 15;67(12). Epub 2022 Jun 15.

MIPAV Lab, School of Electronic and Information Engineering, Soochow University, Jiangsu 215006, People's Republic of China.

. Retinal fluid mainly includes intra-retinal fluid (IRF), sub-retinal fluid (SRF) and pigment epithelial detachment (PED), whose accurate segmentation in optical coherence tomography (OCT) image is of great importance to the diagnosis and treatment of the relative fundus diseases.. In this paper, a novel two-stage multi-class retinal fluid joint segmentation framework based on cascaded convolutional neural networks is proposed. In the pre-segmentation stage, a U-shape encoder-decoder network is adopted to acquire the retinal mask and generate a retinal relative distance map, which can provide the spatial prior information for the next fluid segmentation. In the fluid segmentation stage, an improved context attention and fusion network based on context shrinkage encode module and multi-scale and multi-category semantic supervision module (named as ICAF-Net) is proposed to jointly segment IRF, SRF and PED.. the proposed segmentation framework was evaluated on the dataset of RETOUCH challenge. The average Dice similarity coefficient, intersection over union and accuracy (Acc) reach 76.39%, 64.03% and 99.32% respectively.. The proposed framework can achieve good performance in the joint segmentation of multi-class fluid in retinal OCT images and outperforms some state-of-the-art segmentation networks.
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http://dx.doi.org/10.1088/1361-6560/ac7378DOI Listing
June 2022

Printed 384-Well Microtiter Plate on Paper for Fluorescent Chemosensor Arrays in Food Analysis.

Chem Asian J 2022 May 25:e202200479. Epub 2022 May 25.

Institute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo, 153-8505, Japan.

We propose a printed 384-well microtiter paper-based fluorescent chemosensor array device (384-well microtiter PCAD) to simultaneously categorize and discriminate saccharides and sulfur-containing amino acids for food analysis. The 384-well microtiter PCAD requiring 1 μL/4 mm of each well can allow high-throughput sensing. The device embedded with self-assembled fluorescence chemosensors displayed a fingerprint-like response pattern for targets, the image of which was rapidly captured by a portable digital camera. Indeed, the paper-based chemosensor array system combined with imaging analysis and pattern recognition techniques not only successfully categorized saccharides and sulfur-containing amino acids but also classified mono- and disaccharide groups. Furthermore, the quantitative detectability of the printed device was revealed by a spike and recovery test for fructose and glutathione in a diluted freshly made tomato juice. We believe that the 384-well microtiter PCAD using the imaging analysis system will be a powerful sensor for multi-analytes at several categorized groups in real samples.
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http://dx.doi.org/10.1002/asia.202200479DOI Listing
May 2022

Two different patterns for the premature ventricular complex induction of a long RP supraventricular tachycardia.

HeartRhythm Case Rep 2022 May 11;8(5):339-342. Epub 2022 Feb 11.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-sen University, Key Laboratory on Assisted Circulation, Ministry of Health, Guangzhou, China.

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http://dx.doi.org/10.1016/j.hrcr.2022.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123318PMC
May 2022

EGFR/BRAF/MEK co-inhibition for EGFR-mutated lung adenocarcinoma patients with an acquired BRAF mutation: a case report and review of literature.

Cancer Drug Resist 2021 1;4(4):1019-1027. Epub 2021 Dec 1.

Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Despite the promising initial anti-tumor efficacy of epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs), most advanced non-small-cell lung cancers (NSCLCs) progress eventually due to therapeutic resistance. V-Raf murine sarcoma viral oncogene homolog B1 (BRAF) mutation has been considered as an uncommon mutation that contributes to acquired resistance for EGFR-TKIs. In the presented case, BRAF mutation was detected as an acquired resistance-mediated mutation in a patient treated with osimertinib (a third-generation EGFR-TKI). The presented patient achieved partial regression and ongoing PFS of four months after the co-inhibition of osimertinib plus dabrafenib (BRAF inhibitor) and trametinib (MEK inhibitor). Our case further enriches the clinical evidence of the efficacy of EGFR/BRAF/MEK co-inhibition in patients with an acquired BRAF mutation, consistent with the review of the literature (eight cases). Additionally, our case highlights the important role of sample type, method, and platform of gene detection in patient management, life quality, and prognosis, as well as the understanding of acquired resistance mechanism.
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http://dx.doi.org/10.20517/cdr.2021.98DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8992450PMC
December 2021

Comparison of laparoscopic versus open radical antegrade modular pancreatosplenectomy for pancreatic cancer: A systematic review and meta-analysis.

Int J Surg 2022 Jul 13;103:106676. Epub 2022 May 13.

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Background: Laparoscopic radical antegrade modular pancreatosplenectomy (l-RAMPS) provides a new surgical approach for patients with pancreatic cancers of the body and tail. However, whether it can achieve comparable outcomes to the open RAMPS (o-RAMPS) remains an issue.

Methods: To evaluate the safety and effectiveness of l-RAMPS, the studies in the databases of Medline, Embase, and the Cochrane Library published before September 13, 2021 were searched and a meta-analysis was performed using the 2020 Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guideline. The perioperative and oncological outcomes were analyzed.

Results: Five retrospective cohorts involving 189 patients were included for final pooled analysis. There were no significant differences in the patients' operation time, intra-abdominal bleeding rate, intra-abdominal infection rate, mild morbidity (Clavien-Dindo classification = 1), moderate to severe morbidity (Clavien-Dindo classification ≥2), overall morbidity, wound infection rate, pancreatic fistula rate, delayed gastric emptying rate, reoperation rate, length of hospital stay, postoperative mortality, R0 resection rate, and 2-year overall survival between the 2 approaches. Besides, l-RAMPS was associated with less blood loss (mean difference (MD) = -232.69, 95% confidence interval (CI) = -316.93 to -148.46, P < 0.00001) and shorter days until oral feeding (MD = -0.79, 95% CI = -1.35 to -0.22, P = 0.006). However, the pooled analysis also indicated a significantly fewer lymph nodes dissected (MD = -3.01, 95% CI = -5.59 to -0.43, P = 0.02) in l-RAMPS approach.

Conclusions: Although l-RAMPS provides similar outcomes associated with benefits of minimal invasiveness compared to o-RAMPS, it harvested significantly fewer lymph nodes which might have potentially negative influence on the patients' long-term survival. L-RAMPS is still in the infancy stage and further investigation is needed to verify its feasibility.
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http://dx.doi.org/10.1016/j.ijsu.2022.106676DOI Listing
July 2022

Translation and validation of the Chinese version of Patient-completed Asthma Knowledge Questionnaire and its implementation in patient education.

J Thorac Dis 2022 Apr;14(4):905-918

Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Poor control of asthma results from many factors, partly due to inadequate knowledge towards asthma among patients. It is necessary to know patients' knowledge level before education. However, there is no accepted instrument to evaluate knowledge of asthma in Chinese patients with asthma. The study aims to develop a Chinese version of Patient-completed Asthma Knowledge Questionnaire (PAKQ) to assess its reliability, validity, and responsiveness for testing its clinical application in Chinese adult patients with asthma.

Methods: After translation, back-translation, and cross-cultural adaptation of the PAKQ into Chinese version, a survey of patients with asthma (n=464) in China was conducted. Demographics and clinical data were collected in addition to questionnaires concerning cognition of asthma, education, history, and asthma control test score. The PAKQ was then completed. 14±4 days after the initial assessment, the participants completed the retested questionnaire and again completed the questionnaire immediately after education. The reliability and the construct validity were evaluated. The optimal cut-off points for predicting disease knowledge among asthma patients were determined using the Youden index method.

Results: The Chinese version of PAKQ showed high internal consistency (Cronbach's alpha =0.888) at baseline and an acceptable 2-week test-retest reliability (ICC =0.932, r=0.874). On the basis of large modification indices (>10), this four-factor questionnaire was found to fit the data satisfactorily (χ/df =1.695, RMSEA =0.039, GFI =0.856, CFI =0.885, and SRMR =0.058). Paired -tests showed significant changes on pre-educational and post-educational tests (t=22.83, df=463, P<0.0001). The optimal cut-off value of the PAKQ total score for assessing patients' knowledge level was 35 points (AUC =0.757).

Conclusions: The Chinese version of the PAKQ questionnaire was developed and validated in terms of reliability and validity as an effective instrument for the insight into asthma knowledge of adult patients with asthma in China. Future research will evaluate the utility of the instrument in clinical practice.
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http://dx.doi.org/10.21037/jtd-21-1604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096276PMC
April 2022

THE APPLICATION OF NEURAL NETWORK TECHNOLOGY BASED ON MEA-BP ALGORITHM IN THE PREDICTION OF MICRODOSIMETRIC QUALITIES.

Radiat Prot Dosimetry 2022 Jun;198(7):405-413

School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, PR China.

The most abundant products of the interaction between radiation and matter are low-energy electrons, and the collisions between these electrons and biomolecules are the main initial source of radiation-based biological damage. To facilitate the rapid and accurate quantification of low-energy electrons (0.1-10 keV) in liquid water at different site diameters (1-2000 nm), this study obtained ${\overline{y}}_{\mathrm{F}}$ and ${\overline{y}}_{\mathrm{D}}$data for low-energy electrons under these conditions. This paper proposes a back-propagation (BP) neural network optimized by the mind evolutionary algorithm (MEA) to construct a prediction model and evaluate the corresponding prediction effect. The results show that the ${\overline{y}}_{\mathrm{F}}$ and ${\overline{y}}_{\mathrm{D}}$ values predicted by the MEA-BP neural network algorithm reach a training precision on the order of ${10}^{-8}$. The relative error range between the prediction results of the validated model and the Monte Carlo calculation results is 0.03-5.98% (the error range for single-energy electrons is 0.1-5.98%, and that for spectral distribution electrons is 0.03-4.4%).
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http://dx.doi.org/10.1093/rpd/ncac062DOI Listing
June 2022

Study on the mechanism of inhibiting patulin production by fengycin.

Open Life Sci 2022 19;17(1):372-379. Epub 2022 Apr 19.

College of Life Science, Shaanxi Normal University, Xi'an, Shaanxi, China.

is the main cause of apple rot. Besides, it can also produce mycotoxin patulin (PAT). Therefore, the search for substances that can inhibit the activity and toxigenicity of has become a hot research topic. This study investigates the inhibitory effects of fengycin on patulin production in was cultured under different environments with different concentrations of fengycin. The patulin content produced per unit weight of mycelium was detected and determined by high pressure liquid chromatography (HPLC). Synergy brands (SYBR) GreenI Real-time PCR was used to detect the expression levels of 6-methylsalicylic acid synthase (6-MSAS) and isoepoxydon dehydrogenase (IDH), which were the key genes of producing patulin of mycelium, in the conditions treated by fengycin and untreated. After fengycin treatments, not only the patulin content in every unit weight of mycelium but also the expression level of 6-MSAS decreased significantly. The expression level of 6-MSAS of treatment was 0.11 folds of control. However, the expression level of IDH treated by fengycin decreased slightly. Fengycin could inhibit the from producing patulin by downregulating the expression of key synthetic genes 6-MSAS.
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http://dx.doi.org/10.1515/biol-2022-0041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019426PMC
April 2022

Intracellular and extracellular untargeted metabolomics reveal the effect of acute uranium exposure in HK-2 cells.

Toxicology 2022 05 4;473:153196. Epub 2022 May 4.

Center for Medical Radiation Biology, 903 Hospital, Institute of Materials, China Academy of Engineering Physics, Mianyang 621907, China. Electronic address:

Uranium exposure poses a serious threat to the health of occupational populations and the public. Although metabolomics is a promising research approach to study the toxicological mechanisms of uranium exposure, in vitro studies using human cells are scarce. Applying cultured cell metabolomics, we exhaustively analyzed the intracellular and extracellular differential metabolites upon uranium exposure and characterized the possible biological effects of uranium exposure on human kidney cells. Uranium exposure significantly induced disturbance in the amino acid biosynthesis and linoleic acid metabolism of the cells. Cells exposed to uranium produce excessive amounts of arachidonic acid, which has the potential to cause oxidative stress and damage cells. The results provide new evidence for an oxidative stress mechanism of uranium-induced renal cell injury. Cell metabolomics has proven to be a useful diagnostic tool to study the molecular mechanisms of uranium poisoning.
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http://dx.doi.org/10.1016/j.tox.2022.153196DOI Listing
May 2022

Targeting tissue-resident memory CD8 T cells in the kidney is a potential therapeutic strategy to ameliorate podocyte injury and glomerulosclerosis.

Mol Ther 2022 May 5. Epub 2022 May 5.

The Key Laboratory of Infection and Immunity of Shandong Province, Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, Shandong 250012, China. Electronic address:

Although tissue-resident-memory T (T) cells, a recently identified non-circulating memory T cell population, play a crucial role in mediating local immune responses and protect against pathogens upon local reinfection, the composition, effector function, and specificity of T cells in the kidney and their relevance for chronic kidney disease remain unknown. In this study, we found that renal tissue displayed high abundance of tissue-resident lymphocytes, and the proportion of CD8 T cells was significantly increased in the kidney from patients and mice with focal segmental glomerulosclerosis (FSGS), diabetic kidney disease (DKD), and lupus nephritis (LN). Mechanistically, IL-15 significantly promoted CD8 T cell formation and activation, thereby promoting podocyte injury and glomerulosclerosis. Interestingly, Sparsentan, the dual angiotensin II (Ang II) receptor and endothelin type A receptor antagonist, can also reduce T cell responses by intervening IL-15 signaling, exploring its new pharmacological functions. Mechanistically, Sparsentan inhibited Ang II or endothelin-1 (ET-1)-mediated IL-15 signaling, thereby further regulating renal CD8 T cell fates. Collectively, our studies provide direct evidence for the pivotal role of renal CD8 T cells in podocyte injury and further strengthen that targeting T cells represents a novel therapeutic strategy for patients with glomerular diseases.
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http://dx.doi.org/10.1016/j.ymthe.2022.04.024DOI Listing
May 2022

Pathogenicity analysis and a novel case report of intronic mutations in CSF1R gene.

Neurocase 2022 Apr 3;28(2):251-257. Epub 2022 May 3.

Department of Neurology, Dalian University Affiliated Xinhua Hospital, Dalian, Liaoning, China.

Colony-stimulating factor 1 receptor-associated leukoencephalopathy (CSF1R-related leukoencephalopathy) is a genetic disorder mutated in a single allele. It is characterized by an adult-onset along with predominantly cognitive impairment, accompanied by neuropsychiatric symptoms as well as motor symptoms such as Parkinsonism. In the current study, we confirmed a case of CSF1R-related leukoencephalopathy pedigree by genetic screening, and a new intron . mutation was detected in affected patients. After reviewing all previous reports of introns, we found that symptoms and clinical manifestations of the patients were typical and met the features of previous intron reports.
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http://dx.doi.org/10.1080/13554794.2022.2071625DOI Listing
April 2022

Comparative Analysis of Anthocyanin Compositions and Starch Physiochemical Properties of Purple-Fleshed Sweetpotato "Xuzishu8" in Desert Regions of China.

Front Plant Sci 2022 12;13:841969. Epub 2022 Apr 12.

Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory of Biology and Genetic Improvement of Sweetpotato, Ministry of Agriculture, Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences, Xuzhou, China.

The present study was undertaken to determine the scope of sweetpotato cultivation in arid regions of China. For this purpose, we investigated yield, anthocyanin compositions and physicochemical properties of starch in purple-fleshed sweetpotato (PFSP) "Xuzishu8" under humid (zi8-X) and arid (zi8-D) environments of China. The experiment was conducted in three replications in both environments during 2019 and 2020. The yield and anthocyanidins contents of PFSP were significantly higher in the arid conditions as compared to humid. Zi8-X and zi8-D both revealed the presence of three anthocyanidins, namely, cyanidin (Cy), peonidin (Pn), and pelargonidin (Pg). Cy and Pn accounted for 36.40 and 63.54% of the total anthocyanidins in zi8-X, while in zi8-D, they were found as 26.13 and 73.80%, respectively. The quantitative analysis of these anthocyanins was performed using HPLC-ESI-MS/MS which revealed eighteen anthocyanins such as nine Cy, eight Pn and one Pg. Out of which, eleven anthocyanins showed a significant difference under both conditions. Starch and amylopectin contents were found to be increased by 15.39 and 4.71%, respectively, while the amylose concentration was reduced by 15.54% under the arid environment. The diameter of the starch granule and the peak viscosity were significantly higher under arid as compared to humid conditions. On the basis of results of this study, it seems quite practicable to develop PFSP cultivation in desert regions.
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http://dx.doi.org/10.3389/fpls.2022.841969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039657PMC
April 2022
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