Publications by authors named "Wei Sun"

3,974 Publications

  • Page 1 of 1

Anti-counterfeiting Behaviour of Organic-Inorganic Hybrid Silica Film with a Chiral Smectic C Structure.

Chem Asian J 2022 Aug 18. Epub 2022 Aug 18.

Soochow University, College of Chemistry, Chemical Engineering and Materials Science, CHINA.

Artificial photonic materials based on chiral liquid-crystalline nanostructures have attracted increasing interests for their wide applications as sensors, anti-counterfeit measures, displays and colour filters. Although the structurally coloured films with chiral nematic structures or blue phases have been prepared, the ones fabricated by fixing chiral smectic C (SmC*) phases have been rarely reported. In this work, organic-inorganic hybrid silica (OIHS) films with a SmC* structure were reported for the first time. An organosilane (CSC) with an enantiotropic SmC* phase was synthesized. The OIHS films with a SmC* structure were fabricated by the polycondensation of CSC under an acidic condition. The colour patterns can be observed in the oblique view, but not be observed in the vertical view, which is mainly due to the light scattering of the nanoparticles on film surface and the selective Bragg reflection of film inside. Therefore, such properties make the OIHS films promising candidates for anti-counterfeiting applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/asia.202200793DOI Listing
August 2022

Double-Balanced Loss for Imbalanced Colorectal Lesion Classification.

Comput Math Methods Med 2022 8;2022:1691075. Epub 2022 Aug 8.

Department of Gastroenterology, Eastern Hospital, Shanghai Sixth People's Hospital, Shanghai 201306, China.

Colorectal cancer has a high incidence rate in all countries around the world, and the survival rate of patients is improved by early detection. With the development of object detection technology based on deep learning, computer-aided diagnosis of colonoscopy medical images becomes a reality, which can effectively reduce the occurrence of missed diagnosis and misdiagnosis. In medical image recognition, the assumption that training samples follow independent identical distribution (IID) is the key to the high accuracy of deep learning. However, the classification of medical images is unbalanced in most cases. This paper proposes a new loss function named the double-balanced loss function for the deep learning model, to improve the impact of datasets on classification accuracy. It introduces the effects of sample size and sample difficulty to the loss calculation and deals with both sample size imbalance and sample difficulty imbalance. And it combines with deep learning to build the medical diagnosis model for colorectal cancer. Experimentally verified by three colorectal white-light endoscopic image datasets, the double-balanced loss function proposed in this paper has better performance on the imbalance classification problem of colorectal medical images.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/1691075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377973PMC
August 2022

Feasibility and preliminary efficacy of tantalum components in the management of acetabular reconstruction following periacetabular oncologic resection in primary malignancies.

Eur J Med Res 2022 Aug 17;27(1):151. Epub 2022 Aug 17.

Department of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.

Background: The aim of the study was to investigate the feasibility and preliminary efficacy of tantalum components utility in the reconstruction of acetabular defects following periacetabular oncologic resection of primary malignancies.

Methods: We prospectively collected a consecutive of 15 cases that were treated with tantalum components for acetabular reconstruction after periacetabular oncologic resection from January 2018 to December 2018. The cohort included 8 male and 7 female patients, with a mean age of 47.6 years (range, 33 to 67 years). Pathology types: chondrosarcoma (n = 9), malignant bone giant cell tumor (n = 3) and osteosarcoma (n = 3). Clinical outcomes, functional and radiographic results were recorded in detail for analysis.

Results: Patients received planned oncologic resection and tantalum components reconstruction without casualty; they were followed up with a mean of 39.7 months (35-45 months). The mean operation time was 4.0 h (3.0-6.0 h), and the mean blood loss was 1260 ml (800-2200 ml). Functional outcomes were assessed by MSTS-93 scale, with an average of 21.8 (12.0-26.0 scores), among which 3 cases were excellent, 11 were good and 1 was fair. The mean Harris Hip Score was 79.1scores (46.0-92.0 scores) at 1-year follow-up postoperatively. 3(3/15, 20.0%) cases experienced postoperative complications: 2 cases with hip dislocation received closed reduction under general anesthesia and were fixed with hip joint abduction braces for 6 weeks; one case had a superficial infection and received debridement with a delayed wound healing. Oncologic prognosis: one case relapsed at 8-month follow-up and received hemi-pelvic amputation; and another osteosarcoma patient experienced relapse with pulmonary metastasis and received further chemotherapy. No prosthetic loosening, displacement or fracture occurred during the follow-up period.

Conclusion: Preliminary results suggested that the use of tantalum components in the management of acetabular reconstruction following periacetabular oncologic resection provided reasonable improvement on functional outcomes and early stability of the prostheses. Porous tantalum components are conducive to bony ingrowth, which is a potential alternative to various existing reconstruction techniques to achieve better functional outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40001-022-00777-xDOI Listing
August 2022

E3 ubiquitin ligase RNF6 promotes antiviral immune responses through enhancing the expression of interferon stimulated genes in myeloid cells.

Clin Immunol 2022 Aug 13:109099. Epub 2022 Aug 13.

Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Southeast University, Nanjing, China; Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, China; Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address:

Interferon signaling is closely associated with clearance of viral infections as well as the development of systemic lupus erythematosus (SLE). Therefore, from a clinical perspective, it is important to identify the key regulators involved in interferon signaling pathways. In this study, we identified that RNF6, as an interferon inducible E3 ubiquitin ligase, promoted the interferon-dependent antiviral response. Knock-down of RNF6 greatly attenuated expression of ISGs and the transcriptional activity of ISRE. Specifically, increased RNF6 expression in myeloid cells of patients with SLE correlated with high expression of ISGs. Our results uncover RNF6 as a positive mediator in the antiviral immune responses and suggest that RNF6 may contribute to predict interferon signaling in SLE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clim.2022.109099DOI Listing
August 2022

Reprogramming barriers in bovine cells nuclear transfer revealed by single-cell RNA-seq analysis.

J Cell Mol Med 2022 Aug 15. Epub 2022 Aug 15.

The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, China.

Many progresses have recently been achieved in animal somatic cell nuclear transfer (SCNT). However, embryos derived from SCNT rarely result in live births. Single-cell RNA sequencing (scRNA-seq) can be used to investigate the development details of SCNT embryos. Here, bovine fibroblasts and three factors bovine iPSCs (3F biPSCs) were used as donors for bovine nuclear transfer, and the single blastomere transcriptome was analysed by scRNA-seq. Compared to in vitro fertilization (IVF) embryos, SCNT embryos exhibited many defects. Abnormally expressed genes were found at each stage of embryos, which enriched in metabolism, and epigenetic modification. The DEGs of the adjacent stage in SCNT embryos did not follow the temporal expression pattern similar to that of IVF embryos. Particularly, SCNT 8-cell stage embryos showed failures in some gene activation, including ZSCAN4, and defects in protein association networks which cored as POLR2K, GRO1, and ANKRD1. Some important signalling pathways also showed incomplete activation at SCNT zygote to morula stage. Interestingly, 3F biPSCNT embryos exhibited more dysregulated genes than SCNT embryos at zygote and 2-cell stage, including genes in KDM family. Pseudotime analysis of 3F biPSCNT embryos showed the different developmental fate from SCNT and IVF embryos. These findings suggested partial reprogrammed 3F biPS cells as donors for bovine nuclear transfer hindered the reprogramming of nuclear transfer embryos. Our studies revealed the abnormal gene expression and pathway activation of SCNT embryos, which could increase our understanding of the development of SCNT embryos and give hints to improve the efficiency of nuclear transfer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jcmm.17505DOI Listing
August 2022

In vitro Studies of Non-Diphtheriae Isolates on Antimicrobial Susceptibilities, Drug Resistance Mechanisms, and Biofilm Formation Capabilities.

Infect Drug Resist 2022 9;15:4347-4359. Epub 2022 Aug 9.

Department of Clinical Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China.

Objective: This study aimed to investigate the antimicrobial susceptibilities, drug resistance mechanisms, and biofilm formation capacities of non-diphtheriae strains isolated from sterile midstream urine of hospitalized patients with clinical urinary tract infections (UTIs).

Methods: A total of 45 non-diphtheriae isolates were recovered from sterile midstream urine. The available data of 45 patients were collected. Minimum inhibitory concentrations (MICs) of 10 commonly used antibiotics were determined. Meanwhile, the molecular resistance mechanisms of each agent were performed through PCR with specific primers. Moreover, the biofilm formation capability of each isolate on abiotic surfaces was detected with the MTT method.

Results: In this study, the most prevalent three species were (15/45, 33.3%), (9/45, 20.0%) and (8/45, 17.8%). These three species also accounted for most renal and ureteral calculi cases. Male patients older than 50 years, especially those with underlying diseases, were more susceptible to non-diphtheriae infection. All the 45 isolates were 100% susceptible to vancomycin and linezolid, but highly resistant to macrolide-lincosamide-streptogramin B (MLSB), fluoroquinolones, tetracyclines and β-lactams with corresponding mechanisms. The detection rate of multidrug-resistant (MDR) non-diphtheriae is 91.1%. All isolates are able to form biofilm on abiotic surfaces, except those of and . Isolates of and possessed the strongest biofilm formation capacity. could form biofilm, but varied greatly among different isolates.

Conclusion: and were the most prevalent species relevant to UTIs. The high occurrence of MDR isolates and high diversities in resistance profiles, and the distinctive abilities of biofilm formation highlighted the urgency for identification to species level. We should pay more attention to the drug resistance profiles of non-diphtheriae , which would help improve empirical antibiotic therapy and reduce drug resistance transmission.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S376328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375566PMC
August 2022

GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization, mitochondria-targeting and -activating properties of human salivary histatin 1.

Int J Oral Sci 2022 Aug 15;14(1):42. Epub 2022 Aug 15.

Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam(ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, Netherlands.

Human salivary histatin 1 (Hst1) exhibits a series of cell-activating properties, such as promoting cell spreading, migration, and metabolic activity. We recently have shown that fluorescently labeled Hst1 (F-Hst1) targets and activates mitochondria, presenting an important molecular mechanism. However, its regulating signaling pathways remain to be elucidated. We investigated the influence of specific inhibitors of G protein-coupled receptors (GPCR), endocytosis pathways, extracellular signal-regulated kinases 1/2 (ERK1/2) signaling, p38 signaling, mitochondrial respiration and Na+/K+-ATPase activity on the uptake, mitochondria-targeting and -activating properties of F-Hst1. We performed a siRNA knockdown (KD) to assess the effect of Sigma-2 receptor (S2R) /Transmembrane Protein 97 (TMEM97)-a recently identified target protein of Hst1. We also adopted live cell imaging to monitor the whole intracellular trafficking process of F-Hst1. Our results showed that the inhibition of cellular respiration hindered the internalization of F-Hst1. The inhibitors of GPCR, ERK1/2, phagocytosis, and clathrin-mediated endocytosis (CME) as well as siRNA KD of S2R/TMEM97 significantly reduced the uptake, which was accompanied by the nullification of the promoting effect of F-Hst1 on cell metabolic activity. Only the inhibitor of CME and KD of S2R/TMEM97 significantly compromised the mitochondria-targeting of Hst1. We further showed the intracellular trafficking and targeting process of F-Hst1, in which early endosome plays an important role. Overall, phagocytosis, CME, GPCR, ERK signaling, and S2R/TMEM97 are involved in the internalization of Hst1, while only CME and S2R/TMEM97 are critical for its subcellular targeting. The inhibition of either internalization or mitochondria-targeting of Hst1 could significantly compromise its mitochondria-activating property.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41368-022-00181-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378733PMC
August 2022

The role of lincRNA-p21 in regulating the biology of cancer cells.

Hum Cell 2022 Aug 15. Epub 2022 Aug 15.

Department of Orthopedics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, Guangdong, China.

Long non-coding RNAs (lncRNAs) are a type of multifunctional endogenous RNA transcript. The dysregulation of lncRNAs is considered to play a role in the initiation and progression of cancer. One such lncRNA, long intergenic non-coding RNA-p21 (lincRNA-p21), was identified in 2010 as a regulator in the p53 pathway and is gradually being identified to play crucial roles in diverse cellular processes. In this review, we have summarised the diverse regulatory functions of lincRNA-p21. For example, lincRNA-p21 has been reported to function as a protein decoy, act as a competitive endogenous RNA, regulate the transcription, regulate the translation processes and exist in the secreted exosomes. Furthermore, we highlight the emerging roles of lincRNA-p21 in cancer cell regulation. Various types of cancers, including colorectal carcinoma, hepatocellular carcinoma and non-small cell lung carcinoma, aberrantly express lincRNA-p21. However, the current understanding of the roles of lincRNA-p21 in cancer remains limited. Therefore, considering its potential as a valuable therapeutic target or biomarker for cancer, more research should be conducted to understand the role of lincRNA-p21 in cancer and other diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13577-022-00768-4DOI Listing
August 2022

Application of plasma metabolome for monitoring the effect of rivaroxaban in patients with nonvalvular atrial fibrillation.

PeerJ 2022 9;10:e13853. Epub 2022 Aug 9.

Department of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Rivaroxaban, an oral factor Xa inhibitor, has been used to treating a series of thromboembolic disorders in clinical practice. Measurement of the anticoagulant effect of rivaroxaban is important to avoid serious bleeding events, thus ensuring the safety and efficacy of drug administration. Metabolomics could help to predict differences in the responses among patients by profiling metabolites in biosamples. In this study, plasma metabolomes before and 3 hours after rivaroxaban intake in 150 nonvalvular atrial fibrillation (NVAF) patients and 100 age/gender-matched controls were analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). When compared with controls, a total of thirteen plasma metabolites were differentially expressed in the NVAF patients. Pathway analysis revealed that purine and lipid metabolism were dysregulated. A panel of three metabolites (17a-ethynylestradiol, tryptophyl-glutamate and adenosine) showed good predictive ability to distinguish nonvalvular atrial fibrillation with an area under the receiver operating characteristic curve (AUC) of 1 for the discovery phase and 1 for validation. Under rivaroxaban treatment, a total of seven metabolites changed, the lipid and glycosylphosphatidylinositol biosynthesis pathways were altered and the panel consisting of avocadene, prenyl glucoside and phosphatidylethanolamine showed predictive ability with an AUC of 0.86 for the discovery dataset and 0.82 for the validation. The study showed that plasma metabolomic analyses hold the potential to differentiate nonvalvular atrial fibrillation and can help to monitor the effect of rivaroxaban anticoagulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.13853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373988PMC
August 2022

Case report: Image guidance retrieval of a foreign body in retropharyngeal space.

Front Surg 2022 27;9:949964. Epub 2022 Jul 27.

Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Ingested foreign bodies fully embedded in retropharyngeal space present a unique challenge, as they can be difficult to locate and visualize classic transoral laryngoscopy or the transcervical approach.

Methods: We retrieved a complete extraluminal chicken bone located in the patient's retropharyngeal space at the level of the C4-C5 spine through a well-designed transcervical approach with a combination of image-guided neck navigation.

Conclusion: A combined use of image-guided neck navigation and a dedicated transcervical approach for location of a foreign body in the retropharyngeal space is practical and available for clinical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fsurg.2022.949964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363669PMC
July 2022

Fundamental research on selective arsenic removal from high-salinity alkaline wastewater.

Chemosphere 2022 Aug 11:135992. Epub 2022 Aug 11.

Lengshuijiang Antimony Capital Environmental Protection Co., Ltd, Lengshuijiang, PR China.

The alkaline leaching process of arsenic-containing solid waste discharged during nonferrous metal smelting affords typical high-salinity alkaline arsenic-containing wastewater (HSAW). In this study, for the first time, Me (Ca and Mg)-AsO-OH-HO and Me (Ca and Mg)-AsO-CO-HO systems are studied based on a thermodynamic equilibrium diagram and an arsenic removal experiment, proving that the removal of arsenic using single metal ions in the presence of CO is infeasible because of carbonate coprecipitation. Based on this observation, a new method that uses magnesium ammonium complex salts (MACSs) for HSAW treatment is proposed. Based on the thermodynamic calculations of the Mg-AsO-NH-CO-HO system and the arsenic removal experiment, carbonate and arsenate can be selectively separated by the formation of magnesium ammonium arsenate (NHMgAsO·6HO). In an arsenic solution containing 150-g/L NaCO, the arsenic removal rate and the arsenic grade of the precipitation product reach 90.16% and 27.13%, respectively, when the molar ratios of Mg/NH:As(V) are 1.8:1 and 2:1, respectively. The proposed method is successfully employed for treating a leaching solution of alkaline arsenic slag discharged during antimony smelting. The findings of this study will broaden the basic theory of HSAW treatment and lay a foundation for the resource treatment of arsenic-containing solid waste.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2022.135992DOI Listing
August 2022

Mechanistic and structure investigation of the KOH activation ZIF-8 derived porous carbon as metal-free for unprecedented peroxymonosulfate activation degradation of bisphenol A.

Chemosphere 2022 Aug 10;307(Pt 3):135961. Epub 2022 Aug 10.

School of Civil Engineering and Architecture, East China Jiaotong University, Nanchang, 330013, Jiangxi Province, China.

The high-performance and free secondary pollution of the catalysts are the most critical issues in the peroxymonosulfate-based advanced oxidation processes (PMS-AOPs). In this research, the KOH was used to activate ZIF-8 derived carbon materials to synthesize the NC-KOH-x (x = 700, 800, 900 °C), which was an effective metal-free PMS activator. As-prepared NC-KOH-x showed significant improvement not only pore structure and BET surface area but also CO groups, and graphite N content, which were beneficial for the adsorptive and oxidative reaction. The NC-KOH-900 as an excellent metal-free carbon-catalyst exhibited considerable reactivity for bisphenol A (BPA) removal in broad pH ranges. Almost 100% of BPA was eliminated using 9 mg NC-KOH-900, 0.5 mM PMS within 60 min. Interestingly, It was found that the BPA removal efficiency by adding PMS after saturated adsorption of NC-KOH-x was better than that by adding NC-KOH-x and PMS simultaneously. Electronic paramagnetic resonance (EPR) and quenching experiments results demonstrated that the BPA degradation relied mainly on the nonradical (O) pathways and the defects (I/I), graphitic nitrogen, pyridinic nitrogen, and CO were verified as leading catalytic sites for BPA degradation via PMS activation. Finally, degradation pathways of BPA were proposed and the Toxicity Estimation Software Tool (T.E.S.T.) result implicated that the intermediates of BPA were environmentally friendly to the microorganism and recycled in the ecosystem. The outcomes of this study illustrated the NC-KOH-x owned many merits of state-of-the-art, eco-friendly, and high-performance for great potential practical application value.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2022.135961DOI Listing
August 2022

Mixed potential type sensor based on GdZrO solid electrolyte and BiVO sensing electrode for effective detection of triethylamine.

J Hazard Mater 2022 Jul 28;440:129695. Epub 2022 Jul 28.

State Key Laboratory of Integrated Optoelectronics, Key Laboratory of Advanced Gas Sensors, Jilin Province, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China; International Center of Future Science, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

Triethylamine (TEA), as a common and widely used industrial raw material, is extremely hazardous to the environment and human health. Therefore, the development of a portable gas-sensing technology for high-efficiency detection of TEA is of great worth for human health and environmental monitoring. In this work, a mixed potential type TEA sensor was initially developed based on pyrochlore GdZrO solid state electrolyte and BiVO sensing electrode. The sensor generates high response values of - 62.2 mV and - 134.4 mV to 5 ppm and 100 ppm TEA at 500 °C, respectively. The response value of the sensor displays a logarithmic linear relationship with the concentration of TEA in the range of 1-100 ppm with the sensitivity of - 50.8 mV/decade. Besides, the sensor shows good response and recovery characteristics, and the response and recovery time to 10 ppm TEA is 10 s and 89 s, respectively. Moreover, the sensor possesses good humidity resistance, reproducibility and stability. The sensing behavior of the sensor is explained by the mixed potential sensing mechanism, which is confirmed by the measurement of the polarization curves. This work provides a good supplement for TEA gas sensor, which holds important application value for the sensitive detection of TEA in the environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2022.129695DOI Listing
July 2022

Application of the Non-dominated Sorting Genetic Algorithm II in Multi-objective Optimization of Orally Disintegrating Tablet Formulation.

AAPS PharmSciTech 2022 Aug 12;23(6):224. Epub 2022 Aug 12.

Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, 510000, China.

In the context of increasing application of modelling methods in the field of pharmaceutics, this study aims to reduce the weight of sildenafil orally disintegrating tablets (ODTs) and optimize their formulation through modelling methods. To achieve the goal, the back-propagation neural network (BPNN)-based non-dominated sorting genetic algorithm II (NSGA-II) was introduced to establish the models and to optimize the percentage of magnesium stearate (MgSt), crospovidone (PVPP), and croscarmellose sodium (CCNa) to obtain satisfactory candidate ODTs. Ultimately, the bioequivalence trial was conducted to verify the effectiveness of the formulation. With the support of the neural network, the model showed satisfactory results in the prediction of hardness and disintegration time of ODTs, and the pareto front obtained by the NSGA-II suggested that there was a strong "competition" between disintegration time and hardness. Since disintegration time should be given the priority, the optimal formulation was determined as 1% MgSt, 6% CCNa, and 2.6% PVPP. The bioequivalence trial results indicated a bioequivalence between the test and the reference formulations of sildenafil, and better medication experience for the test formulation. A bioequivalent formulation with better medication experience is successfully prepared using the NSGA-II. It proves that the NSGA-II is applicable to multi-objective optimization of the drug formulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1208/s12249-022-02379-6DOI Listing
August 2022

PBX1-SIRT1 Positive Feedback Loop Attenuates ROS-Mediated HF-MSC Senescence and Apoptosis.

Stem Cell Rev Rep 2022 Aug 12. Epub 2022 Aug 12.

Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Avenue, Changchun, 130021, Jilin, China.

Stem cell senescence and depletion are major causes of aging and aging-related diseases. The NAD (Nicotinamide adenine dinucleotide) - SIRT1 (Silent Information Regulator 1) - PARP1 (Poly (ADP-ribose) polymerase-1) axis has gained interest owing to its significant role in regulating stem cell senescence and organismal aging. A recent study from our lab showed that pre-B-cell leukemia transcription factor1 (PBX1) overexpression attenuates hair follicle-derived mesenchymal stem cells (HF-MSCs) senescence and apoptosis by regulating ROS-mediated DNA damage via PARP1 downregulation; thus, suggesting that PARP1 downregulation is a common manifestation of the roles of both PBX1 and SIRT1 in HF-MSCs senescence attenuation, and implying a potential link between PBX1 and SIRT1. To this end, HF-MSCs overexpressing PBX1, overexpressing both PBX1 and PARP1, downregulating SIRT1, and overexpressing PBX1 as well as downregulating SIRT1 were generated, and senescence, apoptosis, DNA damage, and repair biomarkers were analyzed. Our results showed that (1) PBX1 overexpression alleviated HF-MSCs senescence and apoptosis accompanied by SIRT1 upregulation, PARP1 downregulation, and increased intracellular NAD and ATP levels. (2) SIRT1 knockdown enhanced cellular senescence and apoptosis, accompanied by increased ROS accumulation, DNA damage aggravation, and decreased intracellular NAD and ATP levels. (3) PBX1 overexpression rescued HF-MSCs senescence and apoptosis induced by SIRT1 knockdown. (4) PBX1 rescued PARP1 overexpression-mediated ATP and NAD depletion, accompanied by increased SIRT1 expression. Collectively, our results revealed that a positive interaction feedback loop exists between PBX1 and SIRT1. To the best of our knowledge we are the first to report that there is a PBX1-SIRT1-PARP1 axis that plays a critical role in alleviating HF-MSCs senescence and apoptosis. We provide a new perspective on the mechanisms underlying stem cell senescence as well as age-related disease prevention and treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12015-022-10425-wDOI Listing
August 2022

Corrigendum to "Insights into the implementation of Fibronectin 1 in the cartilage tissue engineering" [Biomed. Pharmacother. 148 (2022) 112782].

Biomed Pharmacother 2022 Aug 9:113539. Epub 2022 Aug 9.

Hand and Foot Surgery Department, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518000, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2022.113539DOI Listing
August 2022

Longitudinal Serum Proteome Characterization of COVID-19 Patients With Different Severities Revealed Potential Therapeutic Strategies.

Front Immunol 2022 26;13:893943. Epub 2022 Jul 26.

State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China.

The COVID-19 pandemic caused by SARS-CoV-2 is exerting huge pressure on global healthcare. Understanding of the molecular pathophysiological alterations in COVID-19 patients with different severities during disease is important for effective treatment. In this study, we performed proteomic profiling of 181 serum samples collected at multiple time points from 79 COVID-19 patients with different severity levels (asymptomatic, mild, moderate, and severe/critical) and 27 serum samples from non-COVID-19 control individuals. Dysregulation of immune response and metabolic reprogramming was found in severe/critical COVID-19 patients compared with non-severe/critical patients, whereas asymptomatic patients presented an effective immune response compared with symptomatic COVID-19 patients. Interestingly, the moderate COVID-19 patients were mainly grouped into two distinct clusters using hierarchical cluster analysis, which demonstrates the molecular pathophysiological heterogeneity in COVID-19 patients. Analysis of protein-level alterations during disease progression revealed that proteins involved in complement activation, the coagulation cascade and cholesterol metabolism were restored at the convalescence stage, but the levels of some proteins, such as anti-angiogenesis protein PLGLB1, would not recovered. The higher serum level of PLGLB1 in COVID-19 patients than in control groups was further confirmed by parallel reaction monitoring (PRM). These findings expand our understanding of the pathogenesis and progression of COVID-19 and provide insight into the discovery of potential therapeutic targets and serum biomarkers worth further validation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.893943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361788PMC
August 2022

Urinary proteomic analysis during pregnancy and its potential application in early prediction of gestational diabetes mellitus and spontaneous abortion.

Ann Transl Med 2022 Jul;10(13):736

Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.

Background: The maternal physiological changes which occur during gestation are complex and affect diverse systems in the body. Elucidating the various changes that occur during pregnancy may assist with understanding maternal health and the factors affecting pregnancy outcomes.

Methods: A longitudinal cohort of 84 pregnant women was established. The urinary proteomes of women in different trimesters of pregnancy (6-8, 22-24, and 32-34 weeks) were characterized using data-independent acquisition tandem mass spectrometry. Gestational diabetes mellitus (GDM) was diagnosed at 24 to 28 weeks. Functional analysis of serial changed proteins was performed.

Results: Fifteen women had GDM, 50 were healthy, and 19 experienced spontaneous abortion (SA). Functional analysis showed that the urinary proteome reflected physiological and pathological changes during pregnancy. Compared to those of women with a normal pregnancy, the urinary proteomes of women with GDM and SA showed significant disease-related changes in insulin secretion and estrogen receptor activity, respectively, during the first trimester. Urinary protein during the first trimester of pregnancy achieved an area under the curve of 0.91 and 0.81 for GDM and SA, respectively.

Conclusions: The urinary proteome has the potential to reflect serial changes of pregnancy progression; therefore, its use might facilitate early diagnosis of pregnancy complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-3497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358500PMC
July 2022

Graph-Based Motion Artifacts Detection Method from Head Computed Tomography Images.

Sensors (Basel) 2022 Jul 28;22(15). Epub 2022 Jul 28.

School of Information Science and Engineering, Northeastern University, Shenyang 110819, China.

Computed tomography (CT) images play an important role due to effectiveness and accessibility, however, motion artifacts may obscure or simulate pathology and dramatically degrade the diagnosis accuracy. In recent years, convolutional neural networks (CNNs) have achieved state-of-the-art performance in medical imaging due to the powerful learning ability with the help of the advanced hardware technology. Unfortunately, CNNs have significant overhead on memory usage and computational resources and are labeled 'black-box' by scholars for their complex underlying structures. To this end, an interpretable graph-based method has been proposed for motion artifacts detection from head CT images in this paper. From a topological perspective, the artifacts detection problem has been reformulated as a complex network classification problem based on the network topological characteristics of the corresponding complex networks. A motion artifacts detection method based on complex networks (MADM-CN) has been proposed. Firstly, the graph of each CT image is constructed based on the theory of complex networks. Secondly, slice-to-slice relationship has been explored by multiple graph construction. In addition, network topological characteristics are investigated locally and globally, consistent topological characteristics including average degree, average clustering coefficient have been utilized for classification. The experimental results have demonstrated that the proposed MADM-CN has achieved better performance over conventional machine learning and deep learning methods on a real CT dataset, reaching up to 98% of the accuracy and 97% of the sensitivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s22155666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371218PMC
July 2022

Small Molecular Drug Screening Based on Clinical Therapeutic Effect.

Molecules 2022 Jul 27;27(15). Epub 2022 Jul 27.

College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Virtual screening can significantly save experimental time and costs for early drug discovery. Drug multi-classification can speed up virtual screening and quickly predict the most likely class for a drug. In this study, 1019 drug molecules with actual therapeutic effects are collected from multiple databases and documents, and molecular sets are grouped according to therapeutic effect and mechanism of action. Molecular descriptors and molecular fingerprints are obtained through SMILES to quantify molecular structures. After using the Kennard-Stone method to divide the data set, a better combination can be obtained by comparing the combined results of five classification algorithms and a fusion method. Furthermore, for a specific data set, the model with the best performance is used to predict the validation data set. The test set shows that prediction accuracy can reach 0.862 and kappa coefficient can reach 0.808. The highest classification accuracy of the validation set is 0.873. The more reliable molecular set has been found, which could be used to predict potential attributes of unknown drug compounds and even to discover new use for old drugs. We hope this research can provide a reference for virtual screening of multiple classes of drugs at the same time in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules27154807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369618PMC
July 2022

Kidney Bean Fermented Broth Alleviates Hyperlipidemic by Regulating Serum Metabolites and Gut Microbiota Composition.

Nutrients 2022 Aug 5;14(15). Epub 2022 Aug 5.

College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China.

Hyperlipidemia with fat accumulation and weight gain causes metabolic diseases and endangers human body health easily which is accompanied by metabolic abnormalities and intestinal flora disorders. In this study, the kidney bean fermented broth (KBF) was used in rats that were fed a high-fat diet to induce hyperlipidemia in order to subsequently analyse the serum metabolomics and gut microbiota modulatoration. The results show that the contents of the total polyphenols and total flavonoids in the KBF were up three and one times, while energy and carbohydrates decreased. In the HFD-induced hyperlipidemic model, body weight, organ weight, and the level of blood lipids (ALT, AST, TG, TC) were lower in rats treated with KBF than in the controls. Metabonomics indicate that there were significant differences in serum metabolomics between the KBF and the HFD. KBF could significantly improve the glycerophospholipids, taurine, and hypotaurine metabolism and amino acid metabolism of hyperlipidemic rats and then improve the symptoms of hypersterol and fat accumulation in rats. The relative abundance of beneficial bacteria increased while pathogenic bacteria decreased after the intervention of KBF. KBF ameliorates dyslipidemia of HFD-induced hyperlipidemic via modulating the blood metabolism and the intestinal microbiota. Collectively, these findings suggest that KBF could be developed as a functional food for anti-hyperlipidemia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu14153202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370468PMC
August 2022

The application of Caprini Risk Assessment Model in evaluation of deep vein thrombosis for patients with end-stage osteoarthritis before arthroplasty.

BMC Musculoskelet Disord 2022 Aug 12;23(1):767. Epub 2022 Aug 12.

State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: Deep vein thrombosis (DVT) was a fatal complication of knee arthroplasty. We had neglected the risk factors of preoperative DVT although patients undergoing knee arthroplasty were at high risk for VTE. This study was to determine the risk factors for preoperative DVT and application of Caprini Risk Assessment Model (RAM) in patients with end-stage knee osteoarthritis (OA).

Methods: We retrospectively analyzed 1808 cases with end-stage knee OA undergoing primary knee arthroplasty from May 2015 to December 2020. Based on the results of ultrasonography in lower extremities, all patients were divided into non-DVT group and DVT group. Distribution of risk factors and risk levels were compared using χ test between two groups. Binary logistic regression analysis was used to determine the risk factors and relationship of risk levels and preoperative DVT.

Results: The incidence of preoperative DVT was 5.53% (n = 100). Distribution of the study population by risk level was low, 4.09%; moderate, 23.95%; high, 66.98%; and highest 4.98%. Female (P = 0.002), age (P = 0.012), swollen legs (P = 0.035) and history of blood clots (P < 0.001) was correlated with preoperative DVT. Difference among four risk levels was significant (P = 0.007). Patients with highest risk level had statistically significant association with preoperative DVT (P = 0.005, OR = 2.93, 95%CI [1.375-6.246]).

Conclusion: The incidence of preoperative DVT was 5.53% in end-stage knee OA patients. The gender (female) and age were independent risk factors for preoperative DVT. The risk group classification by Caprini RAM was significantly associated with preoperative DVT. The usage of Caprini RAM before knee arthroplasty may be beneficial for prophylaxis of DVT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12891-022-05712-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373495PMC
August 2022

Application of DNA barcoding to the entire traditional Chinese medicine industrial chain: A case study of Rhei Radix et Rhizoma.

Phytomedicine 2022 Aug 2;105:154375. Epub 2022 Aug 2.

Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Engineering Research Center of Chinese Medicine Resource of Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China. Electronic address:

Background: Safety concerns, caused by complex and unpredictable adulterants, run through the entire industrial chain of traditional Chinese medicines (TCMs). However, the conventional circulation traceability system only focuses on a certain end or link at the back end of the TCM industrial chain, ignoring the integrity of the links cross the entire industrial chain and lacking traceability. In consequence, a strict and rational supervision system is urgently required for the entire industrial chain.

Hypothesis/purpose: We hypothesize that DNA barcoding would be a suitable measure for the traceability of adulterants in the entire TCM industrial chain.

Methods: In this study, Rhei Radix et Rhizoma was selected as a model to establish a traceability system for the entire TCM industrial chain. A total of 110 samples, including leaves, seeds, roots, decoction pieces, and traditional Chinese patent medicines (TCPMs), were collected upstream, midstream, and downstream of the entire industrial chain of Rhei Radix et Rhizoma. The ndhF-rpl32 fragment rather than the universal DNA barcodes, which could not distinguish the three original species of Rhei Radix et Rhizoma, was selected as a specific DNA barcode to evaluate the practical application of DNA barcoding in the chain.

Results: The results showed that the ndhF-rpl32 fragment in all samples could be amplified and bi-directionally sequenced. Based on the standard operating procedures of DNA barcoding, the ndhF-rpl32 fragment clearly distinguished the seven Rheum species collected upstream of the entire industrial chain. For the samples collected midstream and downstream of the entire industrial chain, 25% of the 36 commercial decoction pieces samples were identified as adulterants, whereas the eight TCPM samples were all derived from genuine Rhei Radix et Rhizoma.

Conclusions: This study shows that DNA barcoding is a powerful and suitable technology that can be applied to trace TCMs in the entire industrial chain, thereby assuring clinical medication safety.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2022.154375DOI Listing
August 2022

HDAC3 Inhibitor RGFP966 Ameliorated Neuroinflammation in the Cuprizone-Induced Demyelinating Mouse Model and LPS-Stimulated BV2 Cells by Downregulating the P2X7R/STAT3/NF-κB65/NLRP3 Activation.

ACS Chem Neurosci 2022 Aug 10. Epub 2022 Aug 10.

Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, Shandong 252000, China.

Suppression of excessive microglial overactivation can prevent the progression of multiple sclerosis (MS). Histone deacetylases 3 inhibitor (HDAC3i) has been demonstrated to exert anti-inflammatory effects by suppressing microglia (M1-liked) activation. Here, we demonstrate that the RGFP966 (a selective inhibitor of HDAC3) protects white matter after cuprizone-induced demyelination, as shown by reductions in neurological behavioral deficits and increases in myelin basic protein. Moreover, in this study, we found that RGFP966 caused a significant reduction in the levels of inflammatory cytokines, including IL-1β, TNF-α, as well as iNOS, and inhibited microglial (M1-liked) activation in the experimental cuprizone model and LPS-stimulated BV2 cells. Meanwhile, RGFP966 alleviated apoptosis of LPS-induced BV2 cells in vitro. Furthermore, RGFP966 suppressed the expression of P2X7R, NLRP3, ASC, IL-18, IL-1β, and caspase-1, inhibited the ratio of phosphorylated-STAT3/STAT3 and phosphorylated NF-κB p65/NF-κB p65, as well as increased acetylated NF-κB p65 in vitro and in vivo. Furthermore, we confirmed that brilliant blue G (antagonists of P2X7R) suppressed the expression of microglial NLRP3, IL-18, IL-1β, caspase-1, NF-κB p65 (including phosphorylated NF-κB p65), and STAT3 (including phosphorylated STAT3) in vitro. These findings demonstrated that RFFP966 alleviated the inflammatory response and exerted a neuroprotective effect possibly by modulating P2X7R/STAT3/NF-κB65/NLRP3 signaling pathways. Thus, HDAD3 might be considered a promising intervention target for neurodegenerative diseases, such as MS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acschemneuro.1c00826DOI Listing
August 2022

The lower limb stiffness, moments and work mode during stair descent among the older adults.

Am J Phys Med Rehabil 2022 Aug 6. Epub 2022 Aug 6.

Shandong Sport University, Jinan, China.

Objective: Lower limb stiffness strategies and work mode changes between young and older adults during stair descent are unclear. This study investigated the effect of aging on the lower limb stiffness, moments and joint work mode during stair descent.

Design: Twenty young adults and Twenty older adults were recruited from the local community for stair descent test. Kinematics and kinetics data were collected by Vicon system and Kistler force plate. The lower limb stiffness, moments and work mode were calculated and assess between groups.

Results: No significant differences in gait parameters were detected between groups. Compared with young adults, older adults have decreased leg stiffness, knee and ankle stiffness, increased peak hip extension moment, hip stiffness and ankle work contribution.

Conclusion: The older adults actively reduce the lower limb stiffness to reduce the risk of injury during stair descent. The hip joint strategy reduces the risk of forwarding falls, and ankle joint compensation work mode to make up for the lack of knee extension strength. This provides a reference for the focus of exercise intervention and rehabilitation strategies for older adults.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/PHM.0000000000002079DOI Listing
August 2022

Exploring the effects of peripheral sensibility on visuospatial and postural capacities during goal-directed movements in long-term Tai Chi practitioners.

Front Aging Neurosci 2022 22;14:881972. Epub 2022 Jul 22.

College of Sports and Health, Shandong Sport University, Jinan, China.

Background: Falls are directly related to visuospatial ability and postural stability. Perturbations of upper body movements pose a challenge to older adults and may cause falls. This study investigated visuospatial ability and postural stability during goal-directed upper body movements between the Tai Chi and control groups and tried to connect them with their sensations.

Materials And Methods: Thirty-seven older adults were recruited to perform the touch (TT) and blind touch (BTT) tasks. The target positioning error (TPE), ankle proprioception, tactile sensation, time to stabilization (TTS), and maximum displacement (D) of the center of pressure trajectory were compared between the groups during the tasks. The relationships of visuospatial ability and postural stability to proprioception and tactile sensation were investigated.

Results: D in the mediolateral (D) direction decreased during BTT compared to TT among the Tai Chi group but not the control group. Compared to the control group, less D in the anterio-posterior (D) direction, and shorter TTS in AP/ML (TTS/TTS) directions were observed among the Tai Chi group. Compared to TT, D decreased during the BTT. The Tai Chi group had less TPE in the vertical (TPE) direction and in three-dimensional space. Among the Tai Chi group, TPE, TTS, and D were correlated to their proprioception during plantarflexion; TTS was correlated to tactile sensation at the great toe during the TT and BTT; D was correlated to tactile sensation at the great toe during the TT. Among the control group, TTS was correlated to ankle proprioception during dorsiflexion and plantarflexion during the BTT.

Conclusion: Long-term Tai Chi practitioners exhibited superior visuospatial ability and postural stability during goal-directed upper body movements, which was associated with sensitive proprioception and tactile sensation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnagi.2022.881972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355721PMC
July 2022

LC-MS-Based Urine Metabolomics Analysis for the Diagnosis and Monitoring of Medulloblastoma.

Front Oncol 2022 22;12:949513. Epub 2022 Jul 22.

Core Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.

Medulloblastoma (MB) is the most common type of brain cancer in pediatric patients. Body fluid biomarkers will be helpful for clinical diagnosis and treatment. In this study, liquid chromatography-mass spectrometry (LC-MS)-based metabolomics was used to identify specific urine metabolites of MB in a cohort, including 118 healthy controls, 111 MB patients, 31 patients with malignant brain cancer, 51 patients with benign brain disease, 29 MB patients 1 week postsurgery and 80 MB patients 1 month postsurgery. The results showed an apparent separation for MB vs. healthy controls, MB vs. benign brain diseases, and MB vs. other malignant brain tumors, with AUCs values of 0.947/0.906, 0.900/0.873, and 0.842/0.885, respectively, in the discovery/validation group. Among all differentially identified metabolites, 4 metabolites (tetrahydrocortisone, cortolone, urothion and 20-oxo-leukotriene E4) were specific to MB. The analysis of these 4 metabolites in pre- and postoperative MB urine samples showed that their levels returned to a healthy state after the operation (especially after one month), showing the potential specificity of these metabolites for MB. Finally, the combination of two metabolites, tetrahydrocortisone and cortolone, showed diagnostic accuracy for distinguishing MB from non-MB, with an AUC value of 0.851. Our data showed that urine metabolomics might be used for MB diagnosis and monitoring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.949513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353006PMC
July 2022

Percutaneous kyphoplasty combined with zoledronic acid for the treatment of primary osteoporotic vertebral compression fracture: a prospective, multicenter study.

Arch Orthop Trauma Surg 2022 Aug 6. Epub 2022 Aug 6.

Department of Orthopedics, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.

Introduction: To explore the therapeutic efficacy of percutaneous kyphoplasty (PKP) combined with zoledronic acid (ZOL) in postmenopausal women and adult men with osteoporotic vertebral compression fracture (OVCF).

Materials And Methods: A total of 238 patients with OVCF were randomly assigned to the control or ZOL group: 119 patients were treated with only PKP (control group), and 119 were treated with ZOL infusion after PKP (ZOL group). Clinical, radiological and laboratory indices were evaluated at follow-up.

Results: The visual analog scale (VAS) score and Oswestry Disability Index (ODI) were significantly higher in both groups post-treatment than at baseline (all p < 0.01). The bone mineral density (BMD) of the proximal femoral neck and height of the injured vertebra were significantly increased after treatment compared with before treatment, and the Cobb angle of the injured vertebra was significantly decreased in both groups (all p < 0.01). However, the bone metabolism indices (type I procollagen amino-terminal peptide (PINP), beta type I collagen carboxy-terminal peptide (β-CTX), and osteocalcin in the N-terminal molecular fragment (NMID)) were significantly lower post-treatment than at baseline in only the ZOL group (all p < 0.01). The VAS score, ODI, BMD, PINP level, β-CTX level, NMID level, vertebral height and Cobb angle of the injured vertebra were significantly higher in the ZOL group than in the control group (all p < 0.01). There were no significant differences in the postoperative bone cement leakage rate between the two groups. At follow-up, new OVCFs were experienced by 16 patients in the control group and 2 patients in the ZOL group (p < 0.01).

Conclusion: The therapeutic efficacy of PKP combined with ZOL for primary OVCF is clinically beneficial and warrants further study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00402-022-04557-4DOI Listing
August 2022

Mowing enhances the positive effects of nitrogen addition on ecosystem carbon fluxes and water use efficiency in a semi-arid meadow steppe.

J Environ Manage 2022 Aug 3;320:115889. Epub 2022 Aug 3.

Institute of Grassland Science, Key Laboratory of Vegetation Ecology of the Ministry of Education, Jilin Songnen Grassland Ecosystem National Observation and Research Station, Northeast Normal University, Changchun, 130024, China. Electronic address:

Grasslands are now facing a continuously increasing supply of nitrogen (N) fertilizers, resulting in alterations in ecosystem functioning, including changes in carbon (C) and water cycling. Mowing, one of the most widely used grassland management techniques, has been shown to mitigate the negative impacts of increased N availability on species richness. However, knowledge of how N addition and mowing, alone and/or in combination, affect ecosystem-level C fluxes and water use efficiency (W) is still limited. We experimentally manipulated N fertilization (0 and 10 g N m yr) and mowing (once per year at the end of the growing season) following a randomized block design in a meadow steppe characterized by salinization and alkalinization in northeastern China. We found that, compared to the control plots, N addition, mowing, and their interaction increased net ecosystem CO exchange by 65.1%, 14.7%, and 133%, and W by 40.7%, 18.5%, and 96.1%, respectively. Nitrogen enrichment also decreased soil pH, which resulted in greater aboveground biomass (AGB). Moreover, N addition indirectly increased AGB by inducing changes in species richness. Our results indicate that mowing enhances the positive effects of N addition on ecosystem C fluxes and W. Therefore, appropriate grassland management practices are essential to improve ecosystem C sequestration, W, and mitigate future species diversity declines due to ecosystem eutrophication.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2022.115889DOI Listing
August 2022

Endocytosis-mediated triple-activable prodrug nanotherapeutics potentiating therapeutic efficacy and security towards solid tumors.

Colloids Surf B Biointerfaces 2022 Jul 30;218:112723. Epub 2022 Jul 30.

Department of Biomedical Engineering, School of Medical Devices, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address:

Self-assembling prodrug nanotherapeutics have emerged as a promising nanoplatform for anticancer drug delivery. The specific and efficient activation of prodrug nanotherapeutics inside tumor cells is vital for the antitumor efficacy and security. Herein, a triple-activable prodrug polymer (TAP) is synthesized by conjugating polyethylene glycol-poly-(caprolactone)-paclitaxel (PTX) polymer with two tumor-responsive bonds, disulfide and acetal. TAP could self-assemble into nanotherapeutics (TAP NTs) free of surfactant with a high drug loading (32.6%). In blood circulation, TAP NTs could remain intact to efficiently accumulate in tumor sites. Thereafter, tumor cells would internalize TAP NTs through multiple endocytosis pathways. Inside tumor cells, TAP NTs could be activated to release PTX and induce tumor cell apoptosis in triple pathways: (i) lysosomal acidity rapid activation; (ii) ROS-acidity tandem activation and (iii) GSH-acidity tandem activation. Compared with Taxol and non-activable control, TAP NTs significantly potentiate the antitumor efficacy and security of PTX against solid tumors including breast cancer and colon cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2022.112723DOI Listing
July 2022
-->