Publications by authors named "Wei Su"

619 Publications

NTM-Based Skill-Aware Knowledge Tracing for Conjunctive Skills.

Comput Intell Neurosci 2022 27;2022:9153697. Epub 2022 Jul 27.

School of Information Science and Engineering, Lanzhou University, Lanzhou 730000, China.

Knowledge tracing (KT) is the task of modelling students' knowledge state based on their historical interactions on intelligent tutoring systems. Existing KT models ignore the relevance among the multiple knowledge concepts of a question and characteristics of online tutoring systems. This paper proposes a neural Turing machine-based skill-aware knowledge tracing (NSKT) for conjunctive skills, which can capture the relevance among the knowledge concepts of a question to model students' knowledge state more accurately and to discover more latent relevance among knowledge concepts effectively. We analyze the characteristics of the three real-world KT datasets in depth. Experiments on real-world datasets show that NSKT outperforms the state-of-the-art deep KT models on the AUC of prediction. This paper explores details of the prediction process of NSKT in modelling students' knowledge state, as well as the relevance of knowledge concepts and conditional influences between exercises.
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http://dx.doi.org/10.1155/2022/9153697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348931PMC
August 2022

The Emerging Role of RNA N6-Methyladenosine Modification in Pancreatic Cancer.

Front Oncol 2022 22;12:927640. Epub 2022 Jul 22.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Pancreatic cancer (PC) is one of the most common malignant cancers, ranking the seventh highest causes of cancer-related deaths globally. Recently, RNA N6-methyladenosine (m6A) is emerging as one of the most abundant RNA modifications in eukaryote cells, involved in multiple RNA processes including RNA translocation, alternative splicing, maturation, stability, and degradation. As reported, m6A was dynamically and reversibly regulated by its "writers", "erasers", and "readers", Increasing evidence has revealed the vital role of m6A modification in the development of multiple types of cancers including PC. Currently, aberrant m6A modification level has been found in both PC tissues and cell lines. Moreover, abnormal expressions of m6A regulators and m6A-modified genes have been reported to contribute to the malignant development of PC. Here in this review, we will focus on the function and molecular mechanism of m6A-modulated RNAs including coding RNAs as well as non-coding RNAs. Then the m6A regulators will be summarized to reveal their potential applications in the clinical diagnosis, prognosis, and therapeutics of PC.
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http://dx.doi.org/10.3389/fonc.2022.927640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354683PMC
July 2022

miR-199a-5p Relieves Obstructive Sleep Apnea Syndrome-Related Hypertension by Targeting HIF-1.

J Immunol Res 2022 27;2022:7236647. Epub 2022 Jul 27.

Cardiovascular Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Introduction: Obstructive sleep apnea syndrome (OSAS) is related to hypertension. Vascular remodeling is both the pathogenesis and the structural change basis of OSAS-related hypertension. Exploring miRNA functioning in OSAS-related hypertension may offer novel diagnostic and therapeutic targets for controlling hypertension-associated cardiovascular diseases. However, the role of miR-199a-5p in OSAS-related hypertension has not been demonstrated yet.

Methods: In this study, we investigated the role of miR-199a-5p and HIF-1 in OSAS-related hypertension by performing cell experiments and animal experiments. Rat aortic smooth muscle cells (A7r5) were cultured under hypoxia as an model. To establish the animal model of OSAS-related hypertension, the rats were under exposure to chronic intermittent hypoxia (CIH) in a hypoxic instrument. The rats were randomly grouped into normal, CIH, CIH+NC, and CIH+miR-199a-5p.

Results: By establishing an animal model, we found decreased miR-199a-5p expression and increased HIF-1 expression in OSAS with hypertension. The overexpressed miR-199a-5p could reduce systolic blood pressure and relieve oxidase stress and inflammation. miR-199a-5p treatment could overturn the upregulation of HIF-1 and TGF-1 and downregulation of -SMA. Overexpressed miR-199a-5p might attenuate vascular remodeling through HIF-1 downregulation. miR-199a-5p/HIF-1 may inhibit proliferation of vascular smooth muscle cells under hypoxia.

Conclusion: miR-199a-5p may relieve OSAS-related hypertension by targeting HIF-1 and be a novel potential therapeutic target.
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http://dx.doi.org/10.1155/2022/7236647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348946PMC
August 2022

Automatic Captioning of Early Gastric Cancer via Magnification Endoscopy with Narrow Band Imaging.

Gastrointest Endosc 2022 Jul 30. Epub 2022 Jul 30.

Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.

Background And Aims: Early gastric cancer (EGC) detection rate is unsatisfactory, and mastering the diagnostic skills of magnifying endoscopy with narrow-band imaging (ME-NBI) requires rich expertise and experience. We aimed to develop an EGC captioning model (EGCCap) to automatically describe the visual characteristics of ME-NBI images for endoscopists.

Methods: A total of 1886 ME-NBI images from 294 cases were enrolled from multi-centers, and corresponding 5658 text data was designed following the simple EGC diagnostic algorithm. An EGCCap was developed using the multi-scale meshed-memory transformer. We conducted comprehensive evaluations for EGCCap including the quantitative and quality performance, generalization, robustness, interpretability, and assistant value analyses. The commonly used metrics including BLEUs, CIDEr, METEOR, ROUGE, SPICE, accuracy, sensitivity, and specificity were used. Two-sided statistical tests were conducted, and statistical significance was determined when P<0.05.

Results: EGCCap acquired satisfying captioning performance by outputting correctly and coherently clinically meaningful sentences in the internal test cohort (BLEU1 = 52.434, CIDEr = 36.734, METEOR = 27.823, ROUGE = 49.949, SPICE = 35.548), and it maintained over 80% performance when applied to other centers or corrupted data. The diagnostic ability of endoscopists improved with the assistance of EGCCap, which was especially significant (P<0.05) for junior endoscopists. Endoscopists gave EGCCap an average remarkable score of 7.182, showing acceptance of EGCCap.

Conclusion: EGCCap exhibited promising captioning performance and was proved with satisfying generalization, robustness, and interpretability. Our study showed potential value in aiding and improving the diagnosis of EGC and facilitating the development of automated reporting in the future.
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http://dx.doi.org/10.1016/j.gie.2022.07.019DOI Listing
July 2022

Preparation of graphene oxide/polydopamine-curcumin composite nanomaterials and its antibacterial effect against Staphylococcus aureus induced by white light.

Biomater Adv 2022 Aug 25;139:213040. Epub 2022 Jul 25.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning 530001, China. Electronic address:

Curcumin (Cur) plays a key role in photodynamic antibacterial activity as a photosensitizer. On the other hand, the antimicrobial potential of graphene oxide (GO) has been reported controversially, and how to improve its antimicrobial ability has become an meaningful study. In this study, we prepared polydopamine-curcumin (PDA-Cur) by pi-pi stacking and loaded it onto the GO surface to obtain GO/PDA-Cur composite nanomaterials. GO/PDA-Cur was characterized by physical and optical means, and GO/PDA-Cur possessed good dispersion and stability in water. In vitro antibacterial results showed that GO/PDA-Cur mediated photodynamic therapy significantly reduced Gram-positive Staphylococcus aureus (S. aureus) by 4 orders of magnitude with a bactericidal rate of 99.99 %. The antibacterial mechanism stems from the fact that GO/PDA-Cur can generate reactive oxygen species (ROS) under white light irradiation (405-780 nm), which causes bacterial outer membrane breakage and cellular deformation. In addition, GO/PDA-Cur has good biocompatibility. The antibacterial ability of graphene oxide was significantly improved by combining it with PDA-Cur, which allows it to be used as a photodynamic antibacterial material.
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http://dx.doi.org/10.1016/j.bioadv.2022.213040DOI Listing
August 2022

Nitrogen-doped carbon dots/curcumin nanocomposite for combined Photodynamic/photothermal dual-mode antibacterial therapy.

Photodiagnosis Photodyn Ther 2022 Jul 26;39:103033. Epub 2022 Jul 26.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning 530001, China. Electronic address:

Due to their excellent photophysical properties, carbon quantum dots have great potential in biomedical and drug delivery fields. In this study, nitrogen-doped carbon quantum dots with good water solubility were prepared using citric acid and ethylenediamine as precursors, and compounded with curcumin, a photosensitive component, to produce composite nanomaterials with photodynamic therapy and synergistic photothermal therapy. The formation of nitrogen-doped carbon quantum dots and composite nanomaterials was verified using physical and optical means. In addition, the composite nanomaterials produced single-linear oxygen and exacerbated the increase of solution temperature under blue (405 nm) and near-infrared (808 nm) light irradiation, respectively. The plate counting method showed that the composite nanomaterials exhibited good photodynamic synergistic photothermal antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus under dual light source (405+808 nm) irradiation, which improved the photoinactivation of curcumin against bacteria. In addition, the composite nanomaterials exhibited low toxicity and good hemocompatibility. These experimental results suggested that the composite nanomaterials showed great potential in a multimodal photodynamic therapy synergistic photothermal treatment platform.
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http://dx.doi.org/10.1016/j.pdpdt.2022.103033DOI Listing
July 2022

The LIM Protein AJUBA is a Potential Oncogenic Target and Prognostic Marker in Human Cancer Pan-Cancer Analysis.

Front Cell Dev Biol 2022 11;10:921897. Epub 2022 Jul 11.

School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.

The LIM (Lin-11, Isl1, MEC-3) domain protein AJUBA is involved in multiple biological functions, and its aberrant expression is related to the occurrence and progression of various cancers. However, there are no analytical studies on AJUBA in pan-cancer. We performed a comprehensive pan-cancer analysis and explored the potential oncogenic roles of AJUBA, including gene expression, genetic mutation, protein phosphorylation, clinical diagnostic biomarker, prognosis, and AJUBA-related immune infiltration based on The Cancer Genome Atlas and Genotype-Tissue Expression databases. The results revealed that the expression of AJUBA highly correlated with poor clinical outcomes in patients with different types of cancer. Meanwhile, AJUBA expression was positively correlated with cancer-associated fibroblasts in many human cancers, such as breast invasive carcinoma, colon adenocarcinoma, brain lower-grade glioma, lung adenocarcinoma (LUAD), and ovarian serous cystadenocarcinoma (OV). Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that AJUBA is mainly involved in protein serine/threonine kinase activity, cell-cell junction, covalent chromatin modification, and Hippo signaling pathway. The pan-cancer study reveals the oncogenic roles of AJUBA and provides a comprehensive understanding of the molecular biological genetic information of AJUBA in various tumors.
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http://dx.doi.org/10.3389/fcell.2022.921897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309301PMC
July 2022

Overexpression of splicing factor poly(rC)-binding protein 1 elicits cycle arrest, apoptosis induction, and p73 splicing in human cervical carcinoma cells.

J Cancer Res Clin Oncol 2022 Jul 27. Epub 2022 Jul 27.

Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.

Purpose: Splicing factor poly(rC)-binding protein 1 (PCBP1) is a novel tumor suppressor that is downregulated in several cancers thereby regulating tumor formation and metastasis. However, the involvement of PCBP1 in apoptosis of cancer cells and the molecular mechanism remains elusive. On this basis, we sought to investigate the role of splicing factor PCBP1 in the apoptosis in human cervical cancer cells.

Methods: To investigate PCBP1 functions in vitro, we overexpressed PCBP1 in human cervical cancer cells. A series of cytological function assays were employed to study to the role of PCBP1 in cell proliferation, cell cycle arrest and apoptosis.

Results: Overexpression of PCBP1 was found to greatly repress proliferation of HeLa cells in a time-dependent manner. It also induced a significant increase in G2/M phase arrest and apoptosis. Furthermore, overexpressed PCBP1 favored the production of long isoforms of p73, thereby inducing upregulated ratio of Bax/Bcl-2, the release of cytochrome c and the expression of caspase-3.

Conclusion: Our results revealed that PCBP1 played a vital role in p73 splicing, cycle arrest and apoptosis induction in human cervical carcinoma cells. Targeting PCBP1 may be a potential therapeutic strategy for cervical cancer therapy.
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http://dx.doi.org/10.1007/s00432-022-04170-3DOI Listing
July 2022

Methylene Blue/Carbon Dots Composite with Photothermal and Photodynamic Properties: Synthesis, Characterization, and Antibacterial Application.

Photochem Photobiol 2022 Jul 25. Epub 2022 Jul 25.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning, China.

Photodynamic therapy and photothermal therapy provide new ways to combat antibiotic resistance. In this research, methylene blue (MB) as an effective photosensitizer was conjugated with carbon quantum dots (CQDs), the composite product not only possessed good antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) due to excellent singlet oxygen ( O ) production rate and light heat transfer performance, but also showed good biocompatibility. Combined with 808 nm and 660 nm laser irradiation, the minimum bactericidal concentration of CQDs-MB towards S. aureus and E. coli was 5 μm. Therefore, this study provides a potential candidate material based on CQDs for clinical applications.
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http://dx.doi.org/10.1111/php.13680DOI Listing
July 2022

Biomechanical and Histological Results of Dual-Suspensory Reconstruction Using Banded Tendon Graft to Bridge Massive Rotator Cuff Tears in a Chronic Rabbit Model.

Am J Sports Med 2022 08 19;50(10):2767-2781. Epub 2022 Jul 19.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Bridging rotator cuff tendon defects with a patch is a reasonable treatment for massive rotator cuff tears (MRCTs). However, the poor outcomes associated with routine patch repair have prompted exploration into superior bridging techniques and graft structures.

Purpose: To detect whether dual-suspensory reconstruction using a banded graft would be superior to routine bridging using a patch graft to treat MRCTs and to detect the comparative effectiveness of patellar tendon (PT) and fascia lata (FL) grafts in dual-suspensory reconstruction.

Study Design: Controlled laboratory study.

Methods: Unilateral chronic MRCTs were created in 72 mature male New Zealand White rabbits, which were randomly divided into 3 groups: (1) patch bridging repair using rectangular FL autograft (PR-FL), (2) dual-suspensory bridging reconstruction using banded FL autograft (DSR-FL), and (3) dual-suspensory bridging reconstruction using banded PT autograft (DSR-PT). In each group, the mean failure load and stiffness of the cuff-graft-humerus (C-G-H) complexes of 6-week and 12-week specimens were recorded, with the failure modes and sites noted. Moreover, cuff-to-graft and graft-to-bone interface healing and graft substance remodeling of the complexes were histologically evaluated (via hematoxylin and eosin, Picrosirius red, Masson trichrome, and Safranin O/fast green staining) at 6 and 12 weeks to assess integrations between the bridging constructs and the native bone or rotator cuff tendons.

Results: The DSR-PT group had the greatest mean failure loads and stiffness of the C-G-H complexes at 6 and 12 weeks (41.81 ± 7.00 N, 10.34 ± 2.68 N/mm; 87.62 ± 9.20 N, 17.98 ± 1.57 N/mm, respectively), followed by the DSR-FL group (32.04 ± 5.49 N, 8.20 ± 2.27 N/mm; 75.30 ± 7.31 N, 14.39 ± 3.29 N/mm, respectively). In the DSR-PT and DSR-FL groups, fewer specimens failed at the graft-to-bone junction and more failed at the cuff-to-graft junction, but both groups had higher median failure loads at 6 and 12 weeks (DSR-PT: cuff-to-graft junction, 37.80 and 83.76 N; graft-to-bone junction, 45.46 and 95.86 N) (DSR-FL: cuff-to-graft junction, 28.52 and 67.68 N; graft-to-bone junction, 37.92 and 82.18 N) compared with PR-FL (cuff-to-graft junction, 27.17 and 60.04 N; graft-to-bone junction, 30.12 and 55.95 N). At 12 weeks, the DSR-FL group had higher median failure loads at graft substance (72.26 N) than the PR-FL group (61.27 N). Moreover, the PR-FL group showed more inflammatory responses at the 2 healing interfaces and the graft substance in the 6-week specimens and subsequently displayed poorer interface healing (assessed via collagen organization, collagen maturity, and fibrocartilage regeneration) and graft substance remodeling (assessed via collagen organization and maturity) in 12-week specimens compared with the DSR-PT and DSR-FL groups. Superior interface healing and substance remodeling processes were observed in the DSR-PT group compared with the DSR-FL group.

Conclusion: When compared with routine patch repair, the dual-suspensory reconstructions optimized biomechanical properties and improved interface healing and graft substance remodeling for bridging MRCTs. Furthermore, the dual-suspensory technique using the PT graft presented superior histological and biomechanical characteristics than that using FL.

Clinical Relevance: The dual-suspensory reconstruction technique using banded tendon grafts may enhance bridging constructs for MRCTs in humans, warranting further investigations of clinical outcomes.
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http://dx.doi.org/10.1177/03635465221102744DOI Listing
August 2022

A systematic pan-cancer analysis identifies RIOK3 as an immunological and prognostic biomarker.

Am J Transl Res 2022 15;14(6):3750-3768. Epub 2022 Jun 15.

Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University Xinxiang 453003, Henan, China.

Objectives: Despite recent research highlighting the critical function of RIO kinase 3 (RIOK3) in a variety of malignancies, a comprehensive evaluation of RIOK3 in human tumors is absent. Our study helps to clarify the molecular mechanism of RIOK3 in carcinogenesis from multiple perspectives.

Methods: Our research looked into the potential oncogenic role of RIOK3 in 33 cancers using TCGA (The Cancer Genome Atlas), GTEx (Genotype-Tissue Expression Project), GEO (Gene Expression Omnibus) datasets, and several bioinformatics tools.

Results: RIOK3 expression in tumors is disordered compared to normal tissue, and it is highly linked with the level of MMR (Mismatch repair) gene mutations and DNA methyltransferase expression. According to univariate survival analysis, it could be used as an independent prognostic factor. Further investigation demonstrated that RIOK3 expression was correlated with cancer-associated fibroblast, neutrophil, and endothelial infiltration levels in kidney cancer and was positively correlated with the expression of immune checkpoint markers in different cancers. The functional pathways of RIOK3 also included cell-cell adhesion, protein phosphorylation, and innate immune-related functions.

Conclusions: These findings suggest that RIOK3 could be used as an immunological and prognostic biomarker in various malignant tumors.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274588PMC
June 2022

Instant diagnosis of gastroscopic biopsy via deep-learned single-shot femtosecond stimulated Raman histology.

Nat Commun 2022 Jul 13;13(1):4050. Epub 2022 Jul 13.

State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Life Sciences, Yiwu Research Institute, Fudan University, 200433, Shanghai, China.

Gastroscopic biopsy provides the only effective method for gastric cancer diagnosis, but the gold standard histopathology is time-consuming and incompatible with gastroscopy. Conventional stimulated Raman scattering (SRS) microscopy has shown promise in label-free diagnosis on human tissues, yet it requires the tuning of picosecond lasers to achieve chemical specificity at the cost of time and complexity. Here, we demonstrate that single-shot femtosecond SRS (femto-SRS) reaches the maximum speed and sensitivity with preserved chemical resolution by integrating with U-Net. Fresh gastroscopic biopsy is imaged in <60 s, revealing essential histoarchitectural hallmarks perfectly agreed with standard histopathology. Moreover, a diagnostic neural network (CNN) is constructed based on images from 279 patients that predicts gastric cancer with accuracy >96%. We further demonstrate semantic segmentation of intratumor heterogeneity and evaluation of resection margins of endoscopic submucosal dissection (ESD) tissues to simulate rapid and automated intraoperative diagnosis. Our method holds potential for synchronizing gastroscopy and histopathological diagnosis.
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http://dx.doi.org/10.1038/s41467-022-31339-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279377PMC
July 2022

Curcumin-loaded multifunctional chitosan gold nanoparticles: An enhanced PDT/PTT dual-modal phototherapeutic and pH-responsive antimicrobial agent.

Photodiagnosis Photodyn Ther 2022 Jul 9;39:103011. Epub 2022 Jul 9.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning 530001, P. R. China. Electronic address:

Overuse of antibiotics has led to the emergence of multidrug resistant (MDR) bacteria.. Photothermal (PTT) and photodynamic therapy (PDT) have may be effective alternatives for antibiotics in the treatment of bacterial infections. In this study, based on chitosan (CS)-coated gold nanoparticles, a pH stimulus-responsive drug delivery system was developed, which can anchor to the cell membrane for photodynamic therapy and photothermal therapy, and enhance the therapeutic potential of curcumin (Cur). Release experiments showed that AuNPs/CS-Cur nanocomposites released curcumin in a pH-dependent manner, which may facilitate the drug to be delivered to the acidic bacterial infection environment. CS as the outer layer covered on gold nanoparticles could improve the dispersibility of Cur in aqueous solution, gold nanoparticles prevent rapid photobleaching of curcumin, thus ensuring the yield of singlet oxygen under irradiation, and enhance the electrostatic binding with bacteria cell membrane. Under light conditions, AuNPs/CS-Cur can produce a large amount of reactive oxygen species and heat to kill S. aureus and E. coli. Compared with free Cur-mediated PDT, the complex significantly improved the synergistic PTT/PDT photoinactivation ability against S. aureus and E. coli. In addition, AuNPs/CS-Cur had good biocompatibility. Therefore, AuNPs/CS-Cur possessed the characteristics of electrostatic targeting, photodynamic and photothermal antibacterial therapy, which would become an efficient and safe antibacterial nano-platform and provide new ideas for the treatment of bacterial infection.
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http://dx.doi.org/10.1016/j.pdpdt.2022.103011DOI Listing
July 2022

Endoscopic ultrasound-guided fine needle aspiration for smooth benign appearing malignant esophageal stricture: a cross-sectional study.

J Thorac Dis 2022 Jun;14(6):2112-2121

Department of Endoscopy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Background: Endoscopic biopsy is standard for the diagnosis of esophageal malignancy. However, few cases are difficult to diagnose as they present with smooth esophageal stricture with negative biopsy results. We aimed to evaluate the effectiveness and safety of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) in the diagnosis of biopsy-negative suspected malignant esophageal strictures.

Methods: We retrospectively analyzed cases of esophageal stricture with negative biopsies. From September 2016 to November 2021, 50 patients were enrolled. All the patients accepted the EUS-FNA examination. And histological and cytological specimens were obtained from all patients. Clinical, endoscopic, imaging, cytological, and histopathological results were noted and analyzed.

Results: A total of 50 patients (40 male and 10 female) were enrolled in this study. The 19G puncture needle was used in 6 cases and the 22G puncture needle was used in 44 cases; an average of 2.7 needles were used per case. Satisfactory specimens were obtained by EUS-FNA for all subjects. All patients were diagnosed as malignant tumor. The diagnosis was confirmed by EUS-FNA biopsies in 98% of patients. Based on the surgical pathology results, there were 16 cases of esophageal squamous cell carcinoma, 2 cases of esophageal metastatic carcinoma, 1 case of esophageal sarcoma, 22 cases of lung cancer, 6 cases of mediastinal lymph node metastasis, and 3 cases of mediastinal tumor. No obvious complications were observed. A total of 5 cases were treated with surgery, 28 with chemotherapy, 3 with chemotherapy + surgery, and 12 with radiotherapy; 2 patients ceased treatment. No obvious complications, such as bleeding and mediastinal infection, were observed.

Conclusions: EUS-FNA is effective and safe for the diagnosis of malignant esophageal strictures with smooth overlying esophageal mucosa. EUS-FNA is effective and safe for patients with smooth esophagus stenosis for whom satisfactory cytological and histological specimens can be obtained, and the diagnosis can be confirmed by cytological, histological, and immunohistochemical examinations. It can be used as the first choice for diagnosis and treatment.
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http://dx.doi.org/10.21037/jtd-22-584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264049PMC
June 2022

Identification of BcARR Genes and CTK Effects on Stalk Development of Flowering Chinese Cabbage.

Int J Mol Sci 2022 Jul 3;23(13). Epub 2022 Jul 3.

College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

Flowering Chinese cabbage ( L. ssp. var. utilis Tsen et Lee) is an important and extensively cultivated vegetable in south China, whose major food product is the stalk. In the process of stalk formation, its initiation and development are regulated by a series of hormonal signals, such as cytokinin and gibberellin. In this study, we analyzed the effects of zeatin (ZT) and gibberellin A3 (GA3), and their interaction, on the bolting of flowering Chinese cabbage. The results indicated that the three-true-leaf spraying of ZT and GA synthesis inhibitor (PAC) inhibited plant height but increased stem diameter. Cytokinin (CTK) synthesis inhibitor (YZJ) and GA3 treatment increased plant height and decreased stem diameter. In addition, ZT and GA3 co-treated plants displayed antagonistic effect. Further, 19 type-B authentic response regulators (ARR-Bs), the positive regulators of cytokinin signal transduction were identified from flowering Chinese cabbage. Comprehensive analysis of phylogeny showed BcARR-Bs clustered into three subfamilies with 10 conserved motifs. Analysis of their expression patterns in different tissues and at various growth stage, and their response to hormone treatment suggest that ARR1-b localized in the nucleus displayed unique highest expression patterns in stem tips, are responsive both to ZT and GA, suggesting a significant role in mediating the crosstalk of ZT and GA in the bolting of flowering Chinese cabbage.
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http://dx.doi.org/10.3390/ijms23137412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266762PMC
July 2022

Application of iron and steel slags in mitigating greenhouse gas emissions: A review.

Sci Total Environ 2022 Jul 6;844:157041. Epub 2022 Jul 6.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, PR China; Guangdong Province Engineering Laboratory for Air Pollution Control, Guangzhou, 510530, PR China. Electronic address:

The comprehensive consideration of climate warming and by-product management in the iron and steel industry, has a significant impact on the realization of environmental protection and green production. Blast furnace slag (BFS) and steel slag (SS), collectively called iron and steel slags, are the main by-products of steelmaking. The economical and efficient use of iron and steel slags to reduce greenhouse gas (GHG) emissions is an urgent problem to be solved. This paper reviewed the carbonization and waste heat recovery of iron and steel slags, and the utilization of iron and steel slags as soil amendments, discussed their application status and limitations in GHG reduction. Iron and steel slags are rich in CaO, which can be used as CO adsorbents to achieve a maximum concentration of 0.4-0.5 kg CO/kg SS. Blast furnace molten slag contains a considerable amount of waste heat, and thermal methods can recover more than 60 % of the heat energy. Chemical methods can use waste heat in the reaction to generate gas fuel, and iron in slags can be used as a catalytic component to promote chemical reaction. Waste heat recovery saves fuel and reduces the CO emissions caused by combustion. When iron and steel slags are used as soil amendments, the iron oxides, alkaline substances, and SiO in iron and steel slags can affect the emission of CH, NO, and CO from soil, microorganisms, and crops, and achieve a maximum reduction of more than 60 % of the overall GHG of paddy fields. Finally, This paper provided valuable suggestions for future GHG reduction studies of iron and steel slags in energy, industry, and agriculture.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157041DOI Listing
July 2022

Mapping the Corn Residue-Covered Types Using Multi-Scale Feature Fusion and Supervised Learning Method by Chinese GF-2 PMS Image.

Front Plant Sci 2022 21;13:901042. Epub 2022 Jun 21.

College of Land Science and Technology, China Agricultural University, Beijing, China.

The management of crop residue covering is a vital part of conservation tillage, which protects black soil by reducing soil erosion and increasing soil organic carbon. Accurate and rapid classification of corn residue-covered types is significant for monitoring crop residue management. The remote sensing technology using high spatial resolution images is an effective means to classify the crop residue-covered areas quickly and objectively in the regional area. Unfortunately, the classification of crop residue-covered area is tricky because there is intra-object heterogeneity, as a two-edged sword of high resolution, and spectral confusion resulting from different straw mulching ways. Therefore, this study focuses on exploring the multi-scale feature fusion method and classification method to classify the corn residue-covered areas effectively and accurately using Chinese high-resolution GF-2 PMS images in the regional area. First, the multi-scale image features are built by compressing pixel domain details with the wavelet and principal component analysis (PCA), which has been verified to effectively alleviate intra-object heterogeneity of corn residue-covered areas on GF-2 PMS images. Second, the optimal image dataset (OID) is identified by comparing model accuracy based on the fusion of different features. Third, the 1D-CNN_CA method is proposed by combining one-dimensional convolutional neural networks (1D-CNN) and attention mechanisms, which are used to classify corn residue-covered areas based on the OID. Comparison of the naive Bayesian (NB), random forest (RF), support vector machine (SVM), and 1D-CNN methods indicate that the residue-covered areas can be classified effectively using the 1D-CNN-CA method with the highest accuracy (: 96.92% and overall accuracy (): 97.26%). Finally, the most appropriate machine learning model and the connected domain calibration method are combined to improve the visualization, which are further used to classify the corn residue-covered areas into three covering types. In addition, the study showed the superiority of multi-scale image features by comparing the contribution of the different image features in the classification of corn residue-covered areas.
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http://dx.doi.org/10.3389/fpls.2022.901042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253822PMC
June 2022

An analysis of the correlation between diabetic retinopathy and preretinal oxygen tension using three-dimensional spoiled gradient-recalled echo sequence imaging.

BMC Med Imaging 2022 07 5;22(1):121. Epub 2022 Jul 5.

Department of Radiology, Affiliated Hospital of Yunnan University, The Second People's Hospital of Yunnan Province, No.176 of Qingnian street, Wuhua District, Kunming, 650000, China.

Background: The aims of this study were to evaluate the levels of preretinal oxygen tension in patients with diabetes who did not have hypertension by using three-dimensional spoiled gradient-recalled (3D-SPGR) echo sequence imaging and to explore the correlation between diabetic retinopathy (DR) and changes in preretinal oxygen tension.

Method: This study involved 15 patients with type 2 diabetes without hypertension, who were divided into a diabetic retinopathy (DR) group (n = 10 eyes) and a diabetic non-retinopathy (NDR) group (n = 20 eyes), according to the results of a fundus photography test. Another healthy control group (n = 14 eyes) also participated in the study. The preretinal vitreous optic disc area, nasal side, and temporal side signal intensity of the eyes was assessed before and after oxygen inhalation with the use of 3D-SPGR echo magnetic resonance imaging (MRI). The signal acquisition time was 10, 20, 30, 40, and 50 min after oxygen inhalation.

Results: The results showed that, in the DR and NDR groups, the preretinal vitreous oxygen tension increased rapidly at 10 min after oxygen inhalation and peaked at 30-40 min, and the increased slope of the DR group was higher than that of the NDR group. The oxygen tension of the preretinal vitreous gradually increased after oxygen inhalation, and the difference between the DR and NDR groups and the control group was statistically significant (P < 0.05). The preretinal vitreous oxygen tension was higher in the optic disc, temporal side, and nasal side in the NDR group than in the control group, and the difference was statistically significant (P < 0.05). The maximum slope ratios of the optic disc and the temporal side of the DR group were greater than those of the control group, and the difference was statistically significant (P < 0.05).

Conclusion: Three-dimensional-SPGR echo MRI sequencing technology is useful for detecting preretinal oxygen tension levels in patients with diabetes. It can be used as one of the functional and imaging observation indicators for the early diagnosis of DR.
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http://dx.doi.org/10.1186/s12880-022-00846-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258100PMC
July 2022

An Arthroscopy-Assisted Mini-Invasive Technique to Create a Chronic Rabbit Model With Massive and Retracted Supraspinatus Rotator Cuff Tears.

Arthrosc Tech 2022 Jun 11;11(6):e999-e1005. Epub 2022 May 11.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Understanding the pathophysiology of rotator cuff tears (RCTs) in animal models is of great importance, as it helps in the development of repair strategies and therapeutic treatments for rotator cuff diseases in humans. This Technical Note describes a comprehensive step-by-step description of an arthroscopic-assisted minimally invasive RCT model in rabbits. This technique is beneficial because the rabbit has rotator cuffs anatomically similar to those of humans, and it has been widely used as a preclinical animal model in the basic science literature. Compared with other small animals (e.g., mice and rats), the advantage of the rabbit model is that it can test the effectiveness and healing process of new surgical repair techniques that require relatively larger anatomical structures. Moreover, it is more cost-effective compared with larger animal models, such as sheep and canines. This arthroscopic-assisted mini-invasive technique to create an RCT model may have a better effect on simulating the degenerative and chronic RCT state in humans than the commonly used open surgery, along with an earlier return to activities, less scarring and tissue adhesion, fewer injuries to the deltoid, and fewer complications.
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http://dx.doi.org/10.1016/j.eats.2022.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244463PMC
June 2022

Arthroscopic Biceps Tenotomy Using a Single Portal for Working and Viewing: A Rabbit Model and Technique.

Arthrosc Tech 2022 Jun 11;11(6):e983-e988. Epub 2022 May 11.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Biceps tenotomy (BT) is a common surgery used to address anterior shoulder pain and joint dysfunction in humans. Using animal models to simulate human conditions is an effective and essential research strategy to further understand histologic and biomechanical processes that occur after BT, including the pathology of the detached biceps, secondary tendinopathic conditions of the rotator cuffs, and glenohumeral functional changes. This Technical Note presents a comprehensive step-by-step description of an arthroscopic BT procedure in rabbits. This technique is particularly beneficial, as the mini-invasive arthroscopic technique in a rabbit model is similar to that performed in humans. which resulted in less scarring and injuries to other adjacent structures in comparison with open surgery.
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http://dx.doi.org/10.1016/j.eats.2022.01.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243671PMC
June 2022

Rose bengal-modified gold nanorods for PTT/PDT antibacterial synergistic therapy.

Photodiagnosis Photodyn Ther 2022 Jun 30;39:102988. Epub 2022 Jun 30.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning, China. Electronic address:

In this study, Rose Bengal (RB) was loaded onto mesoporous silica coated gold nanorods ([email protected]) to form a novel multifunctional platform for antimicrobial therapy ([email protected]). The platform combines the photothermal functions of AuNR and the photodynamic functions of RB to effectively inactivate bacteria under irradiation. Moreover, [email protected] showed negligible cytotoxicity and good blood compatibility. Therefore, this work has potential significance for the development of new antibacterial agents.
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http://dx.doi.org/10.1016/j.pdpdt.2022.102988DOI Listing
June 2022

Evaluation of contrast-induced acute kidney injury using IVIM and DKI MRI in a rat model of diabetic nephropathy.

Insights Imaging 2022 Jun 29;13(1):110. Epub 2022 Jun 29.

Department of Radiology, The Affiliated Hospital of Yunnan University, NO.176 Qingnian Road, Kunming, 650021, Yunnan, China.

Objective: To assess the potential of intravoxel incoherent motion (IVIM) and diffusion kurtosis imaging (DKI) in monitoring renal changes in a diabetic nephropathy (DN) rat model with acute kidney injury (CI-AKI) induced by iso-osmotic contrast media (IOCM) and low-osmotic contrast media (LOCM).

Methods: A diabetic nephropathy rat model was established, and the animals were randomly split into the LOCM group and IOCM group (n = 13 per group), with iopamidol and iodixanol injection, respectively (4 g iodine/kg). MRI including IVIM and DKI was performed 24 h before contrast medium injections (baseline) and 1, 24, 48, and 72 h after injections. Changes in pure molecular diffusion (D), pseudo-diffusion coefficient (D*), perfusion fraction (f), mean diffusion (MD), mean kurtosis (MK), serum creatinine (SCr) and urea nitrogen (BUN), histopathology alterations, and α-smooth muscle actin (α-SMA) expression were assessed. Inter-observer agreement was evaluated using the intraclass correlation coefficient (ICC).

Results: Compared against baseline levels, significant decreases in D, D*, and f were observed in all anatomical kidney compartments after contrast injection (p < 0.05). MD in the cortex (CO) and outer medullary (OM) gradually decreased, and MK in OM gradually increased 24-72 h after injection. D, D*, f, and MD were negatively correlated with the histopathologic findings and α-smooth muscle actin (α-SMA) expression in all anatomical kidney compartments. Inter-observer reproducibility was generally good (ICCs ranging from 0.776 to 0.979).

Conclusions: IVIM and DKI provided noninvasive imaging parameters, which might offer effective detection of CI-AKI in DN.
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http://dx.doi.org/10.1186/s13244-022-01249-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243200PMC
June 2022

The Plug-Type Patch Results in Immediate and Postoperative Advantages in Graft-to-Bone Integration for Bridging Massive Rotator Cuff Tears in a Chronic Rabbit Model.

Am J Sports Med 2022 07 20;50(9):2497-2507. Epub 2022 Jun 20.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Various patches have been used to bridge massive rotator cuff tears (MRCTs) by reconnecting the cuff tendons to the humeral head, but the outcomes continue to be suboptimal. Notably, the graft-bone junction is a vulnerable site for failure, which requires optimization in patch design and techniques to enhance initial and postoperative fixation strength at the graft-bone interface.

Hypothesis: The plug-type patch (Plug-Pat) through intratunnel fixation would optimize mechanical characteristics in initial graft-to-bone fixation and subsequently improve postoperative biomechanical and histological properties in graft-to-bone healing when compared with the routine rectangular patch (Rect-Pat).

Study Design: Controlled laboratory study.

Methods: A total of 60 mature male New Zealand White rabbits underwent acute rotator cuff defects to create chronic models with MRCTs. The fascia lata autograft was then harvested to prepare a Plug-Pat, which was distally rooted in the bone tunnel and proximally sutured to native tendons in a horizontal mattress fashion to reconnect the humeral head and cuff tendons. The control group was repaired with a routine Rect-Pat that was secured onto the bone surface for graft-bone fixation. After surgery, the cuff-graft-bone complexes of rabbits in both groups were harvested immediately (0 weeks) for time-zero initial fixation strength and refreshed contact area assessment, and at 6 or 12 weeks for postoperative biomechanical and histological evaluation.

Results: The Plug-Pat significantly enhanced initial fixation strength in comparison with the Rect-Pat (mean ± SD; failure load, 36.79 ± 4.53 N vs 24.15 ± 2.76 N; < .001) and decreased failure at the graft-bone interface of the construct at 0 weeks, with a significantly increased refreshed bone bed contact area (52.63 ± 2.97 mm vs 18.28 ± 1.60 mm; < .001) between the graft and bone. At 6 and 12 weeks postoperatively, the Plug-Pat similarly resulted in greater failure load (43.15 ± 4.53 N vs 33.74 ± 2.58 N at 6 weeks; = .001; 76.65 ± 5.04 N vs 58.17 ± 5.06 N at 12 weeks; < .001) and stiffness (10.77 ± 2.67 N/mm vs 8.43 ± 0.86 N/mm at 6 weeks; = .066; 16.98 ± 2.47 N/mm vs 13.21 ± 1.66 N/mm at 12 weeks; = .011), with less specimen failure at the graft-bone interface than the Rect-Pat. In histological analyses, the Plug-Pat had a higher postoperative graft-bone integration score than the Rect-Pat, showing a more mature intratunnel healing interface with fibrocartilage tidemark formation, improved collagen properties, and more oriented cells when compared with those at the surface healing interface in the Rect-Pat.

Conclusion: The Plug-Pat enhanced initial fixation strength and enlarged the refreshed contact area for graft-bone connection at time zero and subsequently improved postoperative biomechanical properties and graft-bone integration at the graft-bone healing interface when compared with the Rect-Pat.

Clinical Relevance: The Plug-Pat using intratunnel fixation may be a promising strategy for patch design to optimize its initial and postoperative graft-bone connection for bridging reconstruction of MRCTs.
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http://dx.doi.org/10.1177/03635465221101416DOI Listing
July 2022

Trihexyltetradecylphosphonium chloride based ratiometric fluorescent nanosensors for multiplex anion discrimination.

Analyst 2022 Jul 12;147(14):3209-3218. Epub 2022 Jul 12.

State Key Laboratory of Analytical Chemistry for Life science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P.R. China.

Compared with the well-studied cations, the development of methods for anion detection is relatively slow due to the anion characteristics such as a complex geometry, strong hydration and a low charge density. Herein, a colorimetric and ratiometric fluorescent anion sensing platform based on trihexyltetradecylphosphonium chloride ([THTP][Cl]) was developed for the first time. Such nanosensors exhibited a pH response of 5-7 as well as a high selectivity to perchlorate. The selectivity behavior followed the Hofmeister series in which lipophilic anions were more readily co-extracted. To deviate from the Hofmeister series, anion ionophores should be introduced for selective complexation of the target anions. As a proof of concept, the organomercury compounds ETH9033 and ETH9009 were employed as model ionophores. The obtained nitrate- and chloride-selective [THTP][Cl]-based nanosensors demonstrated prominent colorimetric and spectroscopic transformations specifically induced by the anion species. The fluorescence (/) and absorbance (/) intensities the logarithm values of anion concentrations proved a high selectivity towards the major anion. The excellent performance such as high selectivity, good sensitivity and fast response times enabled the accurate determination of nitrate in mineral water. More importantly, through simply altering the ionophores, a pool of [THTP][Cl]-based anion-selective nano-optodes for extended targets could be achieved. The nanosensor shows great potential for anion determination in the environmental and biomedical fields.
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http://dx.doi.org/10.1039/d2an00735eDOI Listing
July 2022

NEK2 promotes the migration and proliferation of ESCC via stabilization of YAP1 by phosphorylation at Thr-143.

Cell Commun Signal 2022 06 15;20(1):87. Epub 2022 Jun 15.

Zhongshan Hospital Fudan University Endoscopy Center, Shanghai, 200032, China.

Background: Esophageal Squamous Cell Carcinoma (ESCC) was characterized as a regional-prevalent and aggressive tumor with high morbidity and mortality. NIMA-related kinase 2 (NEK2) is an interesting oncogene, the alteration of which leads to patients-beneficial outcomes. We aimed to explore the role of NEK2 in ESCC and excavate its mechanism.

Methods: RNA-seq data were downloaded from TCGA and GEO and analyzed by R software. The protein levels were detected by immunohistochemistry (IHC) or western blot (WB), and mRNA expression was detected by qRT-PCR. The in vitro role of proliferation and migration was detected by Transwell migration assay and by colony formation assay, respectively. The in vivo roles were explored using a subcutaneous xenograft tumor model, where immunofluorescence (IF) and IHC were employed to investigate expression and localization. The interaction between proteins was detected by immunoprecipitation. The stability of proteins was measured by WB in the presence of cycloheximide.

Results: A higher level of NEK2 was found in ESCC than normal esophageal epithelia in GEO, TCGA, and tissue microarray, which was associated with worse prognoses. The NEK2 knockdown impaired the proliferation and migration of ESCC, which also downregulated YAP1 and EMT markers like N-cadherin and Vimentin in vitro. On the contrary, NEK2 overexpression enhanced the migration of ESCC and elevated the levels of YAP1, N-cadherin, and Vimentin. Additionally, the overexpression of YAP1 in NEK2 knocked down ESCCs partly rescued the corresponding decrease in migration. The knockdown of NEK2 played an anti-tumor role in vivo and was accompanied by a lower level and nucleus shuffling of YAP1. In mechanism, NEK2 interacted with YAP1 and increased the stability of both endogenous and exogenous YAP1 by preventing ubiquitination. Moreover, the computer-predicted phosphorylation site of YAP1, Thr-143, reduced the ubiquitination of HA-YAP1, strengthened its stability, and thus influenced the migration in vitro.

Conclusions: NEK2 is a prognostic oncogene highly expressed in ESCC and promotes the progression of ESCC in vitro and in vivo. Mechanistically, NEK2-mediated phosphorylation of YAP1 at Thr-143 protects it from proteasome degradation and might serve as a promising therapeutic target in ESCC. Video Abstract.
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http://dx.doi.org/10.1186/s12964-022-00898-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199137PMC
June 2022

Proteomic Profiling of Cryoglobulinemia.

Front Immunol 2022 23;13:855513. Epub 2022 May 23.

Department of Laboratory Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College Hospital, Beijing, China.

Objective: We aimed to explore and identify candidate protein biomarkers of cryoglobulinemia (CGE) in disease control patients with negative cryoglobulin (DC) or healthy controls (HCs).

Methods: The tandem mass tag (TMT)-labeled serum quantitative proteomics approach was used to identify differentially expressed proteins between the CGE and DC groups. Ingenuity pathway analysis was used for functional annotation of differentially expressed proteins. Biomarker candidates were validated in another cohort using the parallel reaction monitoring (PRM) method. Apolipoprotein A1 (APOA1), apolipoprotein CIII (APOC3), adiponectin, and proprotein convertase subtilisin/kexin type-9 (PCSK9), which represent key proteins involved in the cholesterol metabolism pathway, were further verified in an increased number of samples by enzyme-linked immunosorbent assay (ELISA).

Results: A total of 1004 proteins were identified, of which 109 proteins were differentially expressed between the CGE and DC groups. These differentially expressed proteins were primarily involved in hepatic fibrosis/hepatic stellate cell activation and immune/inflammation-related pathways. In the disease and biofunction analysis, these proteins were mainly associated with the adhesion of blood cells, leukocyte migration, cholesterol transport, and transport of lipids. Twelve candidate biomarkers were validated by PRM-based proteomics, and proteins involved in the cholesterol metabolism pathway were further verified. APOA1, APOC3, adiponectin and PCSK9 concentrations were increased in CGE patients compared with healthy controls (P=0.0123, 0.1136, 0.5760, and 0.0019, respectively).

Conclusion: This report describes the first application of a TMT-PRM-ELISA workflow to identify and validate CGE-specific biomarkers in serum. APOA1 and PCSK9 have been confirmed to be increased in CGE patients, demonstrating that proteins involved in cholesterol metabolism are also implicated in the development of CGE. These findings contribute to pathogenesis research and biomarker discovery in CGE.
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http://dx.doi.org/10.3389/fimmu.2022.855513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167934PMC
May 2022

Systematic analyses identify the anti-fibrotic role of lncRNA TP53TG1 in IPF.

Cell Death Dis 2022 Jun 4;13(6):525. Epub 2022 Jun 4.

Zhuhai People's Hospital, Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai Hospital Affiliated With Jinan University, Jinan University, Zhuhai, 519000, Guangdong, China.

Long non-coding RNA (lncRNA) was reported to be a critical regulator of cellular homeostasis, but poorly understood in idiopathic pulmonary fibrosis (IPF). Here, we systematically identified a crucial lncRNA, p53-induced long non-coding RNA TP53 target 1 (TP53TG1), which was the dysregulated hub gene in IPF regulatory network and one of the top degree genes and down-regulated in IPF-drived fibroblasts. Functional experiments revealed that overexpression of TP53TG1 attenuated the increased expression of fibronectin 1 (Fn1), Collagen 1α1, Collagen 3α1, ACTA2 mRNA, Fn1, and Collagen I protein level, excessive fibroblasts proliferation, migration and differentiation induced by TGF-β1 in MRC-5 as well as PMLFs. In vivo assays identified that forced expression of TP53TG1 by adeno-associated virus 5 (AAV5) not only prevented BLM-induced experimental fibrosis but also reversed established lung fibrosis in the murine model. Mechanistically, TP53TG1 was found to bind to amount of tight junction proteins. Importantly, we found that TP53TG1 binds to the Myosin Heavy Chain 9 (MYH9) to inhibit its protein expression and thus the MYH9-mediated activation of fibroblasts. Collectively, we identified the TP53TG1 as a master suppressor of fibroblast activation and IPF, which could be a potential hub for targeting treatment of the disease.
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http://dx.doi.org/10.1038/s41419-022-04975-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166247PMC
June 2022

Investigation of epidemiological characteristics and development of a nomogram to predict survival in primary ocular adnexal lymphoma.

Clin Exp Ophthalmol 2022 Aug 5;50(6):615-631. Epub 2022 Jul 5.

Department of Hematology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Background: Studies on the epidemiology and prognosis of primary ocular adnexal lymphoma (POAL) are scarce for its low occurrence. The goal of our research was to assess the epidemiologic characteristics, prognostic variables and survival of POAL patients.

Methods: The Surveillance, Epidemiology and End Results (SEER) database was used to collect data on patients identified with POAL from 1975 to 2011 and the incidence rate of POAL from 1975 to 2017. To discover independent predictive markers for overall survival (OS) and disease-specific survival (DSS), multivariable Cox regression analysis was utilised. The independent prognostic factors found by multivariate Cox regression analysis were used to generate a nomogram.

Results: A total of 2839 patients were diagnosed with POAL, with an average age of 64.1 years. The total incidence of POAL was 2.51/1000000 (according to the overall adjustment of the 2000 American standard) from 1975 to 2017, and the annual percentage change (APC) was 2.47 (95% confidence interval 1.64-3.32, p < 0.05), showing a sharp upward trend. After multivariate Cox regression analysis, age, gender, year of diagnosis, marital status, primary site, laterality, pathological type and treatment strategy were evaluated as independent prognostic factors of OS or DSS (p < 0.05). A nomogram was constructed to forecast the DSS of 1, 3, 5 and 10 years. The concordance index (C-index) and the calibration plots demonstrated the robustness and accuracy of the nomogram.

Conclusions: Although POAL is sporadic, the incidence has generally increased in the past 36 years. In recent years, survival rates have risen, and radiotherapy can render better OS and DSS. The nomogram specially made for POAL is robust and precise in predicting the DSS of 1, 3, 5 and 10 years.
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http://dx.doi.org/10.1111/ceo.14122DOI Listing
August 2022

MUC3A promotes the progression of colorectal cancer through the PI3K/Akt/mTOR pathway.

BMC Cancer 2022 Jun 2;22(1):602. Epub 2022 Jun 2.

Department of Oncology, Fudan University Pudong Medical Center, Shanghai Pudong Hospital, 2800 Gongwei Road, Shanghai, 201399, China.

Mucin 3A (MUC3A) is overexpressed in colorectal cancer (CRC) and associated with poor prognosis, but the related mechanism remains unclear. Our study found that MUC3A promotes the progression of CRC by activating the PI3K/Akt/mTOR signaling pathway. Knockout of MUC3A significantly inhibited the proliferation of CRC cells and induced G1 phase arrest by upregulating p21 protein, an important cell cycle regulator. Moreover, knockout of MUC3A significantly inhibited invasion ability and enhanced the sensitivity to the chemotherapeutic agent 5-FU. Furthermore, we found that knockout of MUC3A repressed the PI3K/Akt/mTOR pathway through RNA-seq. Treatment with the PI3K/Akt/mTOR pathway inhibitor rapamycin successfully eliminated the difference in proliferation, invasion and chemoresistance between MUC3A knockout cells and control cells. Our study suggests that MUC3A is a potential oncogene that promotes the proliferation, invasion, and chemotherapy resistance of CRC. Moreover, CRC patients with high expression of MUC3A may benefit from rapamycin treatment.
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http://dx.doi.org/10.1186/s12885-022-09709-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161576PMC
June 2022

Advances in Regenerative Sports Medicine Research.

Front Bioeng Biotechnol 2022 13;10:908751. Epub 2022 May 13.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Regenerative sports medicine aims to address sports and aging-related conditions in the locomotor system using techniques that induce tissue regeneration. It also involves the treatment of meniscus and ligament injuries in the knee, Achilles' tendon ruptures, rotator cuff tears, and cartilage and bone defects in various joints, as well as the regeneration of tendon-bone and cartilage-bone interfaces. There has been considerable progress in this field in recent years, resulting in promising steps toward the development of improved treatments as well as the identification of conundrums that require further targeted research. In this review the regeneration techniques currently considered optimal for each area of regenerative sports medicine have been reviewed and the time required for feasible clinical translation has been assessed. This review also provides insights into the direction of future efforts to minimize the gap between basic research and clinical applications.
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http://dx.doi.org/10.3389/fbioe.2022.908751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136559PMC
May 2022
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