Publications by authors named "Wei Qin"

1,510 Publications

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[Preparation of liquid crystal-based molecularly imprinted monolith and its molecular recognition thermodynamics].

Se Pu 2021 Nov;39(11):1171-1181

Tianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.

Molecularly imprinted polymers (MIPs) incorporated with liquid crystalline monomers can imprint and recognize templates at a very low level of crosslinking, thus addressing challenges associated with conventional MIPs, such as the embedding of the imprinted sites, low binding capacity, and slow mass transfer due to the high degree of crosslinking. Compared with traditional MIPs, the prepared MIPs have a greater number of easily binding sites, which can effectively overcome the embedding and low utilization of imprinting sites. Simultaneously, with a decrease in the level of chemical crosslinking, the mass transfer of template molecules can be significantly improved. However, the imprinting effect of liquid crystalline MIPs is generally weaker than that of traditional MIPs due to the low degree of crosslinking. Therefore, to obtain liquid crystalline MIPs with a good imprinting effect, a series of low-crosslinked liquid crystalline molecularly imprinted monoliths were prepared by graft polymerization and evaluated by high performance liquid chromatography (HPLC) to systematically determine the relation between the polymerization parameters and the affinity of the resulting liquid crystalline MIPs. In this experiment, trimethylolpropane trimethacrylate (TRIM) was used to synthesize a monolithic column skeleton with toluene and dodecyl alcohol as porogens. ()-Naproxen was used as a template and liquid crystalline monomer 4-(4-cyanophenyl)-cyclohexyl ethylene (CPCE) was added for grafting to synthesize the liquid crystalline MIP monolith. The influence of the acetonitrile content and pH in the mobile phase on the chromatographic retention of the template molecule was investigated. The results showed that the main force of MIP recognizing naproxen changed from hydrogen bonding to hydrophobic interaction by the addition of the liquid crystalline monomer. Frontal analysis and adsorption isotherm fitting, including Langmuir, Freundlich, and Scatchard fitting, showed that when the crosslinking degree was 15%, the liquid crystalline MIPs exhibited the highest imprinting factor and heterogeneity, and the specific adsorption was stronger than non-specific adsorption. By analyzing the stoichiometric displacement model, the total affinity of the MIP monoliths for the template molecules (ln ) was determined to be 0.645, significantly higher than that of its analogues, indicating that the liquid crystalline imprinted monolith had a higher total affinity for the template molecule. The spatial matching degree () of the template molecule to the cavity structures of MIPs was also very high, and only inferior to that of ketoprofen. Nevertheless, the ln value of ketoprofen was only 0.242, which indicated that the spatial effect was not the key factor in determining the recognition ability of liquid crystalline imprinting systems. An analysis of the separation thermodynamics revealed that the separation of the liquid crystalline MIPs was an entropy-controlled process, while that of conventional liquid crystalline-free MIPs was an enthalpy-controlled process. Based on the above results, the addition of a liquid crystalline monomer may alter the recognition mechanism of MIPs, and an appropriately low crosslinking degree can significantly improve the recognition performance of liquid crystalline MIPs, paving the way for a new generation of MIPs.
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http://dx.doi.org/10.3724/SP.J.1123.2021.01017DOI Listing
November 2021

MoS -Based Catalysts for N Electroreduction to NH - An Overview of MoS Optimization Strategies.

ChemistryOpen 2021 Oct;10(10):1041-1054

Collaborative Innovation Centre for Green Chemical Manufacturing and Accurate Detection School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, P.R. China.

The nitrogen reduction reaction (NRR) has become an ideal alternative to the Haber-Bosch process, as NRR possesses, among others, the advantage of operating under ambient conditions and saving energy consumption. The key to efficient NRR is to find a suitable electrocatalyst, which helps to break the strong N≡N bond and improves the reaction selectivity. Molybdenum disulfide (MoS ) as an emerging layered two-dimensional material has attracted a mass of attention in various fields. In this minireview, we summarize the optimization strategies of MoS -based catalysts which have been developed to improve the weak NRR activity of primitive MoS . Some theoretical predictions have also been summarized, which can provide direction for optimizing NRR activity of future MoS -based materials. Finally, an outlook about the optimization of MoS -based catalysts used in electrochemical N fixation are given.
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http://dx.doi.org/10.1002/open.202100196DOI Listing
October 2021

A sandwiched photoelectrochemical biosensing platform for detecting Cytokeratin-19 fragments based on AgS-sensitized BiOI/BiS heterostructure amplified by sulfur and nitrogen co-doped carbon quantum dots.

Biosens Bioelectron 2021 Oct 11;196:113703. Epub 2021 Oct 11.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China. Electronic address:

A sandwiched photoelectrochemical (PEC) immunosensor based on BiOI/BiS/AgS was designed for the quantitative detection of cytokeratin-19 fragments (CYFRA21-1) in serum. In this work, due to the intervention of the narrow band gap BiS, the absorption of the light source by the BiOI/BiS heterostructure has been significantly enhanced. Meanwhile, the matched band structure of BiOI, BiS and AgS promoted the rapid transfer of electrons between the conduction bands and effectively inhibited the recombination of electron-hole pairs, thus enhanced the photoelectric signals. Sulfur and nitrogen co-doped carbon quantum dots (S,N-CQDs) with up-conversion luminescence properties provided more light energy for the base materials. On the other hand, S,N-CQDs were combined with Ab through polydopamine (PDA), as secondary antibody labels, further enhanced the sensitivity of the sensor. Herein, the linear range of the sensor was from 0.001 to 100 ng mL and the detection limit was 1.72 pg mL. In addition, the sensor provides a feasible way for the detection of tumor markers due to its excellent selectivity, repeatability and good stability.
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http://dx.doi.org/10.1016/j.bios.2021.113703DOI Listing
October 2021

A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

Plant Methods 2021 Oct 16;17(1):106. Epub 2021 Oct 16.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Background: The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua transient expression, achieving high-throughput gene functional characterization basing on a fast and easy-manipulated transient transformation system in A. annua remains challenging.

Results: The first pair of true leaves of A. annua is an ideal candidate for Agrobacterium injection. EHA105 was the optimal strain that can be used for the development of the transient expression system. The supplementation of Triton X-100 at a concentration of 0.005% greatly improved the transient expression frequency. According to the histochemical β-Glucuronidase (GUS) staining assay, high transient expression level of the reporter gene (GUS) maintained at least a week. Dual-luciferase (Dual-LUC) transient assays showed that the activity of cauliflower mosaic virus 35S (CaMV35S) promoter and its derivates varied between A. annua and tobacco. In A. annua, the CaMV35S promoter had comparable activity with double CaMV35S promoter, while in tobacco, CaMV35S exhibited approximately 50% activity of double CaMV35S promoter. Otherwise, despite the CaMV35S promoter and double CaMV35S promoter from GoldenBraid Kit 2.0 displayed high activity strength in tobacco, they demonstrated a very low activity in transiently expressed A. annua. The activity of UBQ10 promoter and endogenous UBQb promoter was investigated as well. Additionally, using our transient expression system, the transactivation of AaGSW1 and AaORA on AaCYP71AV1 promoter was confirmed. Dual-LUC assays demonstrated that AaHD8 activated the expression of two glandular secreting trichomes-specific lipid transfer protein genes AaLTP1 and AaLTP2, indicating that AaLTP1 and AaLTP2 might serve as downstream components of AaHD8-involved glandular trichome initiation and cuticle formation, as well as artemisinin secretion in A. annua.

Conclusions: A simple, rapid, good-reproducibility, high-efficiency and low-cost transient transformation system in A. annua was developed. Our method offered a new way for gene functional characterization studies such as gene subcellular localization, promoter activity and transcription activation assays in A. annua, avoiding the aberrant phenotypes resulting from gene expression in a heterologous system.
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http://dx.doi.org/10.1186/s13007-021-00807-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520255PMC
October 2021

Effect of Thermo-Mechanical Treatment on the Microstructure and Tensile Properties of the Fe-22Cr-5Al-0.1Y Alloy.

Materials (Basel) 2021 Sep 30;14(19). Epub 2021 Sep 30.

State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou 730050, China.

Oxide dispersion strengthened ferritic steel is considered an important structural material in fusion reactors due to its excellent resistance to radiation and oxidation. Fine and dispersed oxides can be introduced into the matrix via the powder metallurgy process. In the present study, large grain sizes and prior particle boundaries (PPBs) formed in the FeCrAlY alloy prepared via powder metallurgy. Thermo-mechanical treatment was conducted on the FeCrAlY alloy. Results showed that microstructure was optimized: the average grain diameter decreased, the PPBs disappeared, and the distribution of oxides dispersed. Both ultimate tensile strength and elongation improved, especially the average elongation increased from 0.5% to 23%.
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http://dx.doi.org/10.3390/ma14195696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510115PMC
September 2021

Ultrasensitive Double-Channel Microfluidic Biosensor-Based Cathodic Photo-electrochemical Analysis via Signal Amplification of [email protected] for Cardiac Troponin I Detection.

Anal Chem 2021 Oct 12. Epub 2021 Oct 12.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, University of Jinan, Jinan 250022, Shandong, China.

Interesting double-channel microfluidic chip integration with a sandwich-type cathodic photo-electrochemical (PEC) biosensor is utilized for ultrasensitive and efficient detection of cardiac troponin I (cTnI) based on a signal amplification strategy. The Pd nanoparticles loading on the I-doped bismuth oxybromide with oxygen vacancies (Pd/I:BiOBr-OVs) as a sensing platform can effectively enhance cathodic photocurrent response by improving the visible light absorption ability with I doping, facilitating the efficiency separation of photogenerated electron-hole pairs with OVs, and increasing the electron-transfer rate with Pd loading, where the photogenerated electron could be captured by dissolved O to boost generation of a superoxide anion radical (O). To further enhance the PEC response, a novel superoxide dismutase loaded on [email protected] ([email protected]) as a signal amplification label is developed for incubating the detection antibody (dAb). It is particularly noteworthy that SOD can effectively catalyze dismutation of the O to produce HO and O, and [email protected] with a good reduction and catalytic property can catalyze the produced HO into HO and O. Then, the produced O that has been dissolved or adsorbed can capture more photogenerated electrons, resulting in more electron-hole pairs to separate, so as to the cathodic photocurrent signal of this system which can be amplified more significantly. Therefore, a signal amplification cathodic PEC biosensor is prepared for sensitively detecting cTnI, in which a good linearity ranging from 0.1 pg/mL to 100 ng/mL with a low detection limit of 0.042 pg/mL is obtained. Furthermore, the proposed biosensor exhibits excellent sensitivity and high selectivity, which could be extended to detect other disease markers in biological analysis and early disease diagnosis.
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http://dx.doi.org/10.1021/acs.analchem.1c02922DOI Listing
October 2021

Electrocatalytic N Reduction on FeS Nanoparticles Embedded in Graphene Oxide in Acid and Neutral Conditions.

ACS Appl Mater Interfaces 2021 Oct 12. Epub 2021 Oct 12.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, P. R. China.

The development of stable, low-cost, and highly efficient electrocatalysts for the N reduction reaction (NRR) process is challenging but crucial for ammonia production. Herein, we demonstrate the synthesis of pyrite nanoparticles wrapped by graphene oxide ([email protected]) acting as a highly efficient NRR catalyst in a wide pH range. The FeS nanoparticles are uniformly dispersed across the GO nanosheet, thus leading to the fine exposure of active sites, the promotion of charge transfer, and the increment of a contact surface area, which are all beneficial for a desired catalyst. In the meantime, the low-coordinated Fe atoms are activated as highly active sites, which is in favor of the enhanced electrochemical performance for the NRR. Furthermore, density functional theory (DFT) calculations illustrated that the high activity of N reduction over the [email protected] catalyst arises from the well-exposed Fe active sites and the increment of charge density at the valence band edge. Benefiting from the well-optimized interface, the barrier of the addition of the first hydrogen atom to N forming *NNH species as the potential-determining step is as low as 0.93 eV in N electroreduction. The electrochemical test results reveal that, as expected, [email protected] exhibits high Faradaic efficiencies (4.7% in 0.1 M HCl solution and 6.8% in 0.1 M NaSO solution) and advanced NH yields (78.6 and 27.9 μg h mg in 0.1 M HCl and 0.1 M NaSO solutions, respectively) in both acid and neutral conditions. This work offers a new avenue for exploring novel electrocatalysts, which has great promise to accelerate the practical application of the NRR.
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http://dx.doi.org/10.1021/acsami.1c15678DOI Listing
October 2021

Intraoperative Indocyanine Green Retention Test of Left Hemiliver in Decision-Making for Patients With Hepatocellular Carcinoma Undergoing Right Hepatectomy.

Front Surg 2021 17;8:709017. Epub 2021 Sep 17.

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, China.

The aim of this study was to select qualified patients with hepatocellular carcinoma (HCC) who underwent right hepatectomy (RH) intraoperative indocyanine green retention test at 15 min (ICG-R15) of the left hemiliver, which prevents severe posthepatectomy liver failure (PHLF). Twenty HCC patients who were preoperatively planned to undergo RH were enrolled. Intraoperative ICG-R15 of left hemiliver was measured after the right Glissonean pedicle was completely blocked. Patients then underwent RH if intraoperative ICG-R15 was ≤ 10%. Otherwise, patients underwent staged RH (SRH), either associating liver partitioning and portal vein ligation for staged hepatectomy (ALPPS) or portal vein ligation (PVL), followed by stage-2 RH. The comparison group consisted of patients with a ratio of standard left liver volume (SLLV) of > 40% and preoperative ICG-R15 ≤ 10% who underwent RH. The clinical outcomes of these two groups were compared. Of the 20 patients, six underwent stage-1 RH, six underwent ALPPS, five underwent PVL followed by stage-2 RH, and three failed to proceed to stage-2 RH after PVL. No significant differences were found among the 17 patients who underwent stage-1 or stage-2 RH in the study group, the 19 patients in the comparison group, the 11 patients in the stage-2 RH group, and the six patients in the stage-1 RH group in incidences of PHLF, postoperative complications, hospital stay, and HCC recurrence within 1 year after RH. Compared with the stage-1 ALPPS group, the mean operative time and blood loss of the stage-1 PVL group were significantly less ( <0.001 and = 0.022, respectively). The stage-1 PVL group had a significantly longer waiting-time (43.4 vs. 14.0 days, = 0.016) than the stage-1 ALPPS group to proceed to stage-2 RH. After stage-2 RH, tumor recurrence within 1 year was 20% (1/5) in patients after PVL and 50% (3/6) after stage-1 ALPPS. Intraoperative ICG-R15 ≤ 10% of left hemiliver was valuable in intraoperative decision-making for patients who were planned to undergo RH. There is a possibility that stage-1 PVL might help to select patients with more favorable biological behavior to undergo stage-2 RH.
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http://dx.doi.org/10.3389/fsurg.2021.709017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484520PMC
September 2021

AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua.

Hortic Res 2021 Oct 1;8(1):217. Epub 2021 Oct 1.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Frontiers Science Center for Transformative Molecules, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both the response to biotic stresses, such as pathogens, and artemisinin biosynthesis. Here, we isolated and identified the WRKY transcription factor (TF) AaWRKY17, which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A. annua. Yeast one-hybrid (Y1H), dual-luciferase (dual-LUC), and electrophoretic mobility shift assay (EMSA) results showed that AaWRKY17 directly bound to the W-box motifs in the promoter region of the artemisinin biosynthetic pathway gene amorpha-4,11-diene synthase (ADS) and promoted its expression. Real-time quantitative PCR (RT-qPCR) analysis revealed that the transcript levels of two defense marker genes, Pathogenesis-Related 5 (PR5) and NDR1/HIN1-LIKE 10 (NHL10), were greatly increased in AaWRKY17-overexpressing transgenic A. annua plants. Additionally, overexpression of AaWRKY17 in A. annua resulted in decreased susceptibility to P. syringae. These results indicated that AaWRKY17 acted as a positive regulator in response to P. syringae infection. Together, our findings demonstrated that the novel WRKY transcription factor AaWRKY17 could potentially be used in transgenic breeding to improve the content of artemisinin and pathogen tolerance in A. annua.
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http://dx.doi.org/10.1038/s41438-021-00652-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484609PMC
October 2021

Microfluidic Ratiometric Photoelectrochemical Biosensor Using a Magnetic Field on a Photochromic Composite Platform: A Proof-of-Concept Study for Magnetic-Photoelectrochemical Bioanalysis.

Anal Chem 2021 10 29;93(40):13680-13686. Epub 2021 Sep 29.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, University of Jinan, Jinan 250022, P. R. China.

Integrating a microfluidic sensor with a ratiometric photoelectrochemical (PEC) strategy to build a bioanalysis device for actual sample testing is often limited to large-volume space-resolution equipment and wavelength-dependent or potential-dependent paired photoactive materials. This work reports a microfluidic ratiometric magnetic-photoelectrochemical (M-PEC) biosensor on the photochromic composite platform to solve the above problems. In particular, as a proof-of-concept study, the platform BiWO/amorphous BiOCl nanosheets/BiS (p-BWO-s) mediated by photochromic color centers and the magnetic photoactive secondary antibody marker [email protected] are integrated on the microfluidic biosensor. By enhancement of the photochromic color centers, p-BWO-s outputs a considerable photocurrent signal. Meanwhile, the photoactivity of the secondary antibody marker can be changed with a magnetic field; thus, different photocurrent signals can be obtained to realize ratiometric detection. The quenching photocurrent signal without the magnetic field and the difference photocurrent signal under the magnetic field are quantitatively related to the target concentration, which unfolds a novel general strategy for bioanalysis. Different from traditional ratiometric PEC biosensors, this work characterizes the first ratiometric PEC biosensor based on an external magnetic field. Generally speaking, combined with different biorecognition cases, this scheme with good expansibility brings a unique new perspective.
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http://dx.doi.org/10.1021/acs.analchem.1c03171DOI Listing
October 2021

Molecular Heterogeneity in Localized Diffuse Large B-Cell Lymphoma.

Front Oncol 2021 7;11:638757. Epub 2021 Sep 7.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine, Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The clinical and molecular characteristics of localized diffuse large B-cell lymphoma (DLBCL) with single nodal (SN) or single extranodal (SE) involvement remain largely elusive in the rituximab era. The clinical data of 181 patients from a retrospective cohort and 108 patients from a phase 3 randomized trial NHL-001 (NCT01852435) were reviewed. Meanwhile, genetic aberrations, gene expression pattern, and tumor immunophenotype profile were revealed by DNA and RNA sequencing of 116 and 53 patients, respectively. SE patients showed similar clinicopathological features as SN patients, except for an increased percentage of low-intermediate risk in the National Comprehensive Cancer Network-International Prognostic Index. According to the molecular features, increased mutations were observed in SN patients, while SE patients were associated with upregulation of TGF-β signaling pathway and downregulation of T-cell receptor signaling pathway. SE patients also presented immunosuppressive status with lower activity of killing of cancer cells and recruiting dendritic cells. Extranodal involvement had no influence on progression-free survival (PFS) or overall survival (OS) in localized DLBCL. Serum lactate dehydrogenase >3 upper limit of normal was an independent adverse prognostic factor for OS, and mutations were related to inferior PFS. Although the overall prognosis is satisfactory, specific clinical, genetic, and microenvironmental factors should be considered for future personalized treatment in localized DLBCL.
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http://dx.doi.org/10.3389/fonc.2021.638757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454464PMC
September 2021

Effects of omega-3 polyunsaturated fatty acids supplementation for patients with cardiovascular disease risks: a dose-response meta-analysis.

Am J Transl Res 2021 15;13(8):8526-8539. Epub 2021 Aug 15.

School of Medicine, Southeast University Nanjing, Jiangsu, P. R. China.

Background: Previous studies assessing the impact of omega-3 polyunsaturated fatty acids (ω-3 PUFA) have shown conflicting results in regard to the cardiovascular mortality. It is likely that higher dose of ω-3 PUFA would have a greater effect on the major adverse cardiovascular events (MACEs). Therefore, we performed a dose-response meta-analysis to explore the potential protective effect of ω-3 PUFA, with the increase of daily intake and extension of the intervention period, on patients with cardiovascular disease risks. Outcomes included major adverse cardiovascular events, cardiovascular and all-cause mortality.

Methods: A systematic literature search of PubMed, Embase and the Cochrane Library from inception to September 31, 2019 was conducted to identify the randomized controlled trails (RCTs) of ω-3 PUFA supplementation, which reported cardiovascular events or deaths and recruited no less than 500 participants. We evaluated the effect of ω-3 PUFA through the pooled relative risks (RR) and 95% confidence intervals (95% CI), and further carried out subgroup analysis and dose-response meta-analysis.

Results: Fourteen trials including 87718 individuals were reviewed. By conventional statistical significance, there was no apparent difference between the two groups on major adverse cardiovascular effects (RR 0.94, 95% CI 0.84-1.04) and all-cause mortality (RR 0.96, 95% CI 0.91-1.00), but there was an effect on the cardiovascular mortality (RR 0.93, 95% CI 0.88-0.99). However, with the dose increased and intervention period prolonged (daily dose × intervention period > 8 grams/day × years), subgroup analyses showed a more obvious reduction of MACEs (RR 0.79, 95% CI 0.65-0.95) and all-cause mortality (RR 0.93, 95% CI 0.85-1.03). Furthermore, the dose-response meta-analysis presented a 13.05% reduction of MACEs and 8.99% reduction of all-cause mortality with 10 grams/day × years increments.

Conclusions: Updated with the newly published RCTs, this meta-analysis indicated that large dose and long period of interventions with ω-3 PUFA supplementation produce a close association with MACEs and cardiovascular or all-cause mortality. A dose-response beneficial effect was preliminarily established.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430075PMC
August 2021

Correlation between bispectral index and prognosis of patients with acute cerebral infarction.

Curr Neurovasc Res 2021 Sep 17. Epub 2021 Sep 17.

Department of Neurology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing. China.

Introduction: This study aimed to investigate the clinical value of bispectral index (BIS) monitoring in assessing the consciousness and prognosis of acute cerebral infarction (ACI) patients.

Methods: In total, 64 patients who suffered from ACI with consciousness disturbance were enrolled in this study. Glasgow Coma Scale (GCS) was performed to evaluate the consciousness level of ACI patients, and BIS was used to monitor the depth of anesthesia and sedation. Then, patients were divided into good prognosis, poor prognosis and death groups according to modified Rankin score (mRS). Discrimination analysis of BIS values and GCS score for the prediction of prognosis was performed using the receiver operator characteristic (ROC) curve.

Results: GCS score and BIS values showed statistically significant differences among the three groups. Spearman rank correlation analysis revealed a significant positive correlation between BIS values and GCS score, while BIS values was negatively related with mRS. The ROC curve of prognosis prediction showed strong prognostic power, with area under the curves (AUCs) between 0.830 and 0.917. Moreover, the AUC of BISmean score was higher than that of BISmax, BISmin and GCS, and BISmean of 74 was the best cut-off point for good prognosis.

Conclusion: BIS directly reflects the degree of consciousness disturbance in ACI patients, and thus accurately predicts the prognosis, indicating potential application values of BIS in clinical practice.
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http://dx.doi.org/10.2174/1567202618666210917164223DOI Listing
September 2021

A dual signal-amplified electrochemiluminescence immunosensor based on core-shell [email protected] nanosphere for procalcitonin detection.

Mikrochim Acta 2021 Sep 16;188(10):344. Epub 2021 Sep 16.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.

A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was fabricated for trace detection of procalcitonin (PCT). [email protected] composed of sea urchin-like [email protected] nanoparticles coated on CeO hollow nanospheres was immobilized on electrode surface to electrochemically catalyze HO to produce a large number of superoxide anion (O). The immunosensor was prepared by linking the capture antibody on immobilized [email protected] with heptapeptide (HWRGWVC), which could maintain the activity of the antibody. The prepared Au [email protected] was used to bind abundant luminol for labeling the secondary antibody (Ab). Upon the sandwich-typed immunoreactions, the O could react with the introduced luminol on the immunosensor surface to produce strong ECL intensity. With an outstanding linear detection range and a low detection limit of 17 fg/mL, the ECL immunosensor permitted ultrasensitive detection of PCT at a low HO concentration and demonstrated its high application potential in the clinical assay.
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http://dx.doi.org/10.1007/s00604-021-04988-7DOI Listing
September 2021

Peptide-Based Electrochemiluminescence Biosensors Using Silver Nanoclusters as Signal Probes and Pd-CuO Hybrid Nanoconcaves as Coreactant Promoters for Immunoassays.

Anal Chem 2021 09 15;93(38):13045-13053. Epub 2021 Sep 15.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China.

Metal nanoclusters (NCs) possess high light stability and biocompatibility because of their unique quantum size effect, which has gradually become a new type of electrochemiluminescence (ECL) nanomaterial for immunoassays. However, the luminescence efficiency of metal NCs is too low to meet the needs of trace analysis, which limits its application. Herein, Ag NCs served as signal probes and Pd-CuO hybrid nanoconcaves served as coreaction promoters, developing a highly efficient peptide-based biosensor for neuron-specific enolase (NSE) detection. Utilizing the reversible cycle of Cu/Cu and the reduction characteristics of Pd NPs, Pd-CuO greatly accelerates the reduction of SO. Meanwhile, Pd-CuO has good hydrogen evolution activity, which promotes the generation of oxygen by improving the redox efficiency of the overall reaction, thus increasing the yield of active intermediates (OH) to promote the reduction of SO. Specially, this is an effective attempt to use the hydrogen evolution reaction (HER) to accelerate the ECL emission of the SO system. In addition, a short peptide ligand (NARKFYKGC, NFC) was developed to implement the targeted immobilization of antibodies, which can specifically bind to the Fc fragment of antibodies, thereby avoiding the occupation of the antigen binding site (Fab fragment). The introduction of NFC not only improves the binding efficiency of antibodies but also protects its bioactivity, thus significantly improving the sensitivity of the biosensor. Based on these strategies, the proposed biosensor provides a new perspective for the applications of metal NCs in ECL systems.
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http://dx.doi.org/10.1021/acs.analchem.1c03002DOI Listing
September 2021

No-wash point-of-care biosensing assay for rapid and sensitive detection of aflatoxin B1.

Talanta 2021 Dec 2;235:122772. Epub 2021 Aug 2.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.

In many cases of in-situ or point-of-care testing (POCT), the single pursuit of good detection performance cannot meet the testing requirements, and thus no-wash testing has become one of the most effective methods to develop sustainable biosensing assay, providing more convenient operation procedures and shorting the detection time. Herein, a disposable POC biosensing assay was prepared based on the RGB color detector software on the smartphone and the peroxide-like activity of gold nanoparticles (Au NPs) for aflatoxin B1 (AFB1) sensitive detection. Using syringe filters for a simple physical separation procedure can easily realize washing free detection, which is superior to most biosensing assays with cumbersome detection procedures. The syringe filters with 200 nm pore diameter could only pass through small Au NPs (30 nm) while the large-sized SiO nanoparticles (300 nm) was blocked on the membrane. In this work, Au NPs utilized their inherent peroxidase-like activity to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO to ox-TMB under acidic conditions, which results in blue color in aqueous solution. The color change due to different antigen concentrations was quantitatively determined by measuring the color intensity with a smartphone and the RGB color detector. By measuring the color intensity, it was known that the linear concentration range of the biosensing assay was 100 fg mL to 50 ng mL, and the detection limit of AFB1 was 33 fg mL (S/N = 3). Additionally, the designed biosensing assay exhibited excellent selectivity, storage stability and reproducibility. More importantly, the innovation of detecting and analyzing technology is the outstanding advantage of the biosensing assay, providing a more flexible and convenient strategy for some other small molecule analysis.
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http://dx.doi.org/10.1016/j.talanta.2021.122772DOI Listing
December 2021

The Association of Morning Hypertension With Target Organ Damage in Patients With Chronic Kidney Disease and Hypertension.

Front Cardiovasc Med 2021 26;8:715491. Epub 2021 Aug 26.

Division of Nephrology, Department of Medicine, West China Hospital, Sichuan University, Chengdu, China.

To determine the association between morning hypertension and target organ damage (TOD) in patients with chronic kidney disease (CKD) and hypertension. In this cross-sectional study, 447 patients with CKD and hypertension from two centers were enrolled. Ambulatory blood pressure monitoring was conducted in all patients. Linear regression and logistic regression analysis were used to determine the association between morning hypertension and TOD in patients with CKD and hypertension, including assessments of estimated glomerular filtration rate (eGFR), left ventricular mass index (LVMI), urine protein/creatinine ratio (UPCR), and left ventricular hypertrophy (LVH). Overall, 194 (43.4%) participants had morning hypertension. Morning hypertension was strongly correlated with LVH [odds ratio (OR), 2.14; 95% confidence interval (CI), 1.3-3.51; < 0.01], lower level of eGFR (β = -0.51; 95%CI, -0.95--0.08; < 0.05), higher LVMI (β = 0.06; 95%CI, 0.04-0.08, < 0.001), and UPCR (β = 0.22; 95%CI, 0.06-0.38, < 0.01), independent of nocturnal hypertension and elevated morning blood pressure surge. As a continuous variable, both morning systolic blood pressure (SBP) and diastolic blood pressure (DBP) were found to be associated with LVH and higher level of UPCR and LVMI ( < 0.05), whereas only morning SBP was negatively correlated with eGFR ( < 0.01). Morning hypertension was strongly correlated with cardiac damage and impaired kidney function in CKD patients with hypertension, independent of nocturnal hypertension and morning surge in blood pressure. Morning hypertension in CKD patients warrants further attention.
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http://dx.doi.org/10.3389/fcvm.2021.715491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427187PMC
August 2021

scAPAdb: a comprehensive database of alternative polyadenylation at single-cell resolution.

Nucleic Acids Res 2021 Sep 11. Epub 2021 Sep 11.

Pasteurien College, Soochow University, Suzhou, Jiangsu 215000, China.

Alternative polyadenylation (APA) is a widespread regulatory mechanism of transcript diversification in eukaryotes, which is increasingly recognized as an important layer for eukaryotic gene expression. Recent studies based on single-cell RNA-seq (scRNA-seq) have revealed cell-to-cell heterogeneity in APA usage and APA dynamics across different cell types in various tissues, biological processes and diseases. However, currently available APA databases were all collected from bulk 3'-seq and/or RNA-seq data, and no existing database has provided APA information at single-cell resolution. Here, we present a user-friendly database called scAPAdb (http://www.bmibig.cn/scAPAdb), which provides a comprehensive and manually curated atlas of poly(A) sites, APA events and poly(A) signals at the single-cell level. Currently, scAPAdb collects APA information from > 360 scRNA-seq experiments, covering six species including human, mouse and several other plant species. scAPAdb also provides batch download of data, and users can query the database through a variety of keywords such as gene identifier, gene function and accession number. scAPAdb would be a valuable and extendable resource for the study of cell-to-cell heterogeneity in APA isoform usages and APA-mediated gene regulation at the single-cell level under diverse cell types, tissues and species.
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http://dx.doi.org/10.1093/nar/gkab795DOI Listing
September 2021

In-Plane Critical Magnetic Fields in Magic-Angle Twisted Trilayer Graphene.

Phys Rev Lett 2021 Aug;127(9):097001

Department of Physics, The University of Texas at Austin, Austin, Texas 78712, USA.

It has recently been shown [Y. Cao, J. M. Park, K. Watanabe, T. Taniguchi, and P. Jarillo-Herrero, Pauli-limit violation and re-entrant superconductivity in moiré graphene, Nature (London) 595, 526 (2021).NATUAS0028-0836] that superconductivity in magic-angle twisted trilayer graphene survives to in-plane magnetic fields that are well in excess of the Pauli limit, and much stronger than the in-plane critical magnetic fields of magic-angle twisted bilayer graphene. The difference is surprising because twisted bilayers and trilayers both support the magic-angle flat bands thought to be the fountainhead of twisted graphene superconductivity. We show here that the difference in critical magnetic fields can be traced to a C_{2}M_{h} symmetry in trilayers that survives in-plane magnetic fields, and also relative displacements between top and bottom layers that are not under experimental control at present. An gate electric field breaks the C_{2}M_{h} symmetry and therefore limits the in-plane critical magnetic field.
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http://dx.doi.org/10.1103/PhysRevLett.127.097001DOI Listing
August 2021

Generating Long-Lived Macroscopically Distinct Superposition States in Atomic Ensembles.

Phys Rev Lett 2021 Aug;127(9):093602

Theoretical Quantum Physics Laboratory, RIKEN Cluster for Pioneering Research, Wako-shi, Saitama 351-0198, Japan.

We propose to create and stabilize long-lived macroscopic quantum superposition states in atomic ensembles. We show that using a fully quantum parametric amplifier can cause the simultaneous decay of two atoms and, in turn, create stabilized atomic Schrödinger cat states. Remarkably, even with modest parameters these intracavity atomic cat states can have an extremely long lifetime, up to 4 orders of magnitude longer than that of intracavity photonic cat states under the same parameter conditions, reaching tens of milliseconds. This lifetime of atomic cat states is ultimately limited to several seconds by extremely weak spin relaxation and thermal noise. Our work opens up a new way toward the long-standing goal of generating large-size and long-lived cat states, with immediate interests both in fundamental studies and noise-immune quantum technologies.
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http://dx.doi.org/10.1103/PhysRevLett.127.093602DOI Listing
August 2021

Decline of three farmland pest species in rapidly urbanizing landscapes.

iScience 2021 Sep 19;24(9):103002. Epub 2021 Aug 19.

Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary, Institute of Biodiversity Science and Institute of Eco-Chongming, School of Life Sciences, Fudan University, Shanghai 200438, China.

Urbanization is a pressing challenge for earth's humans because it is changing not only natural environments but also agricultural lands. Yet, the consequences of cropland loss on pest insect populations that largely depend on these habitats remain largely unclear. We used a 17-year data set to investigate the dynamics of three moth pest species (i.e., striped stem borer, yellow stem borer, and pink stem borer) and their driving forces across the largest mega-urban region of China. Total abundance of three pest species is declined by about 80%, which was strongly associated with cropland loss during rapid urbanization. Our findings indicate that not only the increasing conversion of natural areas to human-dominated landscapes but also that of agricultural lands to urban landscapes can be critical to insect populations. It is therefore essential to monitor and understand the insect dynamics in rapidly urbanizing regions, which are currently found in many developing countries worldwide.
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http://dx.doi.org/10.1016/j.isci.2021.103002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411231PMC
September 2021

Synchronous organ failure and infected pancreatic necrosis define genuine critical acute pancreatitis.

Dig Liver Dis 2021 Sep 6. Epub 2021 Sep 6.

Department of Pancreatic Surgery, General Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan Province 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China. Electronic address:

Background: Critical acute pancreatitis (CAP) was supposed to be strongly associated with the highest risk of adverse outcomes. However, the definition of CAP needs to be further clarified.

Methods: A prospective database with consecutive patients of infected pancreatic necrosis (IPN) at a tertiary hospital was post-hoc analyzed. Patients were assigned to IPN alone, Metachronous-CAP (MCAP) and Synchronous-CAP group (SCAP) according to presence or absence of organ failure (OF) and the crosstalk between OF and IPN. Clinical interventions and outcomes were compared among groups.

Results: A total of 248 IPN patients were enrolled and the overall mortality was 25.8%. Compared with MCAP, SCAP was associated with higher mortality (66.2 versus 10.0%) and morbidity (41.2 versus 18.0%), longer duration of OF (median 35.5 versus 12.0 days), ICU length of stay (LOS) (median 28.0 versus 16.0 days) and hospital LOS (median 67.0 versus 60.0 days) (all P < 0.05). The IPN alone and MCAP had comparable mortality (10.8 versus 10.0%), morbidity and hospital LOS, except that MCAP patients were characterized with longer duration of OF and ICU LOS (P < 0.05).

Conclusions: SCAP, characterized with synchronous persistent OF and IPN, was associated with higher mortality and morbidity and should be defined as genuine CAP.
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http://dx.doi.org/10.1016/j.dld.2021.08.016DOI Listing
September 2021

Pilot-scale bioaugmentation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil using an indigenous bacterial consortium in soil-slurry bioreactors.

Chemosphere 2021 Sep 4;287(Pt 2):132183. Epub 2021 Sep 4.

Engineering Research Center of Ministry of Education on Groundwater Pollution Control and Remediation, College of Water Sciences, Beijing Normal University, Beijing, 100875, PR China. Electronic address:

Soil-slurry bioreactor based bioremediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil was studied through laboratory and pilot-scale trials, in which the degradation mechanism was explored. Indigenous PAH-degrading consortium was firstly screened out and it degraded 80.5% of total PAHs in lab-scale bioreactors. Then a pilot-scale trial lasting 410 days was conducted in two bioreactors of 1.5 m to examine the operating parameters and validate the optimum running conditions. During the initial 200 days, the crucial running parameters affecting PAH removal were evaluated and selected. Subsequently, an average PAH removal rate of 93.4% was achieved during 15 consecutive batches (210 days) under the optimum running conditions. The kinetic analysis showed that the reactor under optimum conditions achieved the highest PAH degradation rate of 0.1795 day and the shortest half-life of 3.86 days. Notably, efficient mass transfer of PAHs and high biodegradation capability by bioaugmented consortia in soil-slurry bioreactors were two key mechanisms for appreciable PAH removal performance. Under the optimal operating conditions, the degradation rate of low-molecular-weight (LMW) PAHs was significantly higher than high-molecular-weight (HMW) PAHs; when the mass transfer was limited, there was no significant difference between their degradation behaviors. Both microbial co-metabolism and collaborative metabolism might occur when all PAHs demonstrated low degradation rates. The findings provide insightful guidance on the future assessment and remediation practices of PAH-contaminated sites.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132183DOI Listing
September 2021

Association of morning blood pressure surge with chronic kidney disease progression in patients with chronic kidney disease and hypertension.

J Clin Hypertens (Greenwich) 2021 Sep 9. Epub 2021 Sep 9.

Division of Nephrology, Department of Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Blood pressure (BP) usually rise from being asleep to awake, which is named the morning blood pressure surge (MBPS). Researches have reported that elevated MBPS was related with CV events, incident CKD in hypertensive patients. However, there have been no studies that have investigated the association between MBPS and renal or heart outcomes in patients with CKD and hypertension, in these patients, the MBPS is much lower because of high prevalence of night hypertension and reduced BP dipping. In this prospective two-center observational study, we enrolled patients with CKD and hypertension and the 24 h ambulatory blood pressure monitoring (ABPM) was conducted in all patients. Time to total mortality, CKD progression and CV events was recorded; Finally, a total of 304 patients were enrolled and 94 (30.9%) of them had elevated MBPS. After a follow-up for median 30 months, 23 (7.6%), 34 (11.2%), and 95 (31.3%) patients occurred death, CKD progression and new-onset CV events, respectively. The Cox regression analysis suggested the elevated MBPS was a strong predictor of CKD progression (HR 2.35, 95%CI 1.2 -4.63, p = .013), independent of morning BP, while no associations were found between elevated MBPS and CV events (HR 1.02, 95%CI 0.66 -1.57), as well as death (HR 1.08, 95%CI 0.46 -2.55). In conclusion, we provided the first evidence that elevated MBPS was an important risk factor of CKD progression in patients with CKD and hypertension. Appropriate evaluation and management of MBPS may be helpful to postpone CKD progression.
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http://dx.doi.org/10.1111/jch.14366DOI Listing
September 2021

Vulnerability of the worldwide air transportation network to global catastrophes such as COVID-19.

Transp Res E Logist Transp Rev 2021 Oct 30;154:102469. Epub 2021 Aug 30.

Department of Business Administration, National Taiwan University, Taipei, Taiwan, ROC.

This paper studies the vulnerability of the worldwide air transportation network (WATN) during a global catastrophe such as COVID-19. Considering the WATN as a weighted network, many airport connections could be completely or partially disrupted during such extreme events. However, it is found that existing weighted metrics cannot reflect the impact of connection capacity reduction on network connectivity. Therein, this work proposes a novel network efficiency metric termed as layered weighted network efficiency (LWNE) metric to measure the connectivity of the air transportation networks (ATNs) and study their vulnerability in response to different levels of disruptions, including airport level, country level, and global level. The most critical airport connections and their impact on network connectivity are identified. It is found that the critical connections are mostly between so-called bridge airports but not core airports in the WATN. By examining the impact of partial link disruptions, it is found that some connections mainly serve local travel demand and are very robust to partial disruptions, while the others connecting global hubs are sensitive to partial disruptions. Further, the WATN is robust to the individual disconnection of most countries; however, it is vulnerable to the simultaneous disconnection of countries that serve international transfers. Interestingly, the WATN is insensitive to the disconnection between any two countries, even those with sizeable domestic ATNs. Concerning global disconnections, as long as all the international connections hold 10% of their original flights, the WATN can still expect 40% of its pre-disruption performance. This paper deepens the understanding of ATNs under extreme events and provides a method for studying transportation networks' vulnerability facing global disruptions.
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http://dx.doi.org/10.1016/j.tre.2021.102469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411847PMC
October 2021

A photoelectrochemical self-powered sensor for the detection of sarcosine based on NiO NSs/PbS/Au NPs as photocathodic material.

J Hazard Mater 2021 08 24;416:126201. Epub 2021 May 24.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

In this study, lead(II) sulfide (PbS) nanocrystals were modified on nickel(II)oxide nanosheets (NiO NSs) via the chemical bath method. Afterwards, Au nanoparticles (NPs) were also modified successfully. A photoelectrochemical (PEC) self-powered platform for detecting sarcosine with high PEC activity was constructed. The capacity of NiO NSs to be loaded with other sensitizing materials was mainly attributed to its porous structure and large specific surface area. Under optimum conditions, the constructed PEC self-powered cathodic sensor for detecting sarcosine exhibited a linear range in 5.0 × 10-5.0 × 10 mol/L with a detection limit (LOD) of 1.7 × 10 mol/L. The biosensor demonstrated good reproducibility, acceptable stability and high specificity, thus confirming its potential application in the detection of other similar substances.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126201DOI Listing
August 2021

Interface engineering of [email protected](OH) nanoarray heterostucture: Electrodeposition of [email protected](OH) as N and H channels for artificial NH synthesis under mild conditions.

J Colloid Interface Sci 2021 Aug 18;606(Pt 2):1374-1379. Epub 2021 Aug 18.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, China; Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Jinan, Shandong 250022, China. Electronic address:

The electrocatalytic reduction of nitrogen (N) to ammonia (NH) has broad prospects for green and sustainable NH production. Due to the electrocatalytic nitrogen reduction reaction (eNRR) performance of transition metal compound may be restricted with low yield rate, we develop transition metal interface engineering to improve the eNRR performance. Although the edge of MoS catalyst is active, the MoS(001) surface is inert for N electroreduction. Through the hydrothermal and electrodeposition methods, Fe(OH) as N and H channels coated on MoS nanosheets array ([email protected](OH)/CC) is synthesized. Such catalyst exhibits excellent eNRR performance in 0.1 M NaSO with high Faradaic efficiency (2.76%) and NH yield rate (4.23 × 10 mol s cm) at - 0.45 V (vs. RHE). This work may provide a new electrocatalyst synthesis pathway for artificial N fixation. Density functional theory calculations show that electrodeposition Fe(OH) can accelerate eNRR process rate of MoS.
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http://dx.doi.org/10.1016/j.jcis.2021.08.099DOI Listing
August 2021

Identification of four Spätzle genes (MnSpz1, MnSpz2, MnSpz2-isoform, and MnSpz3) and their roles in the innate immunity of Macrobrachium nipponense.

Dev Comp Immunol 2022 Jan 1;126:104254. Epub 2021 Sep 1.

Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China. Electronic address:

Spätzle, an extracellular ligand of the Toll receptor, is involved in the innate immunity of crustaceans. In this study, four Spätzle genes were cloned from Macrobrachium nipponense and designed as MnSpz1, MnSpz2, MnSpz2-isoform, and MnSpz3. The coding region of the four Spätzle genes all contained one intron and two exons, and they were predicted to be produced by gene duplication based on sequence similarities and phylogenetic tree. The predicted MnSpz1, MnSpz2, and MnSpz3 proteins all contained a signal peptide and a Spätzle domain. No signal peptide but a Spätzle domain existed in MnSpz2-isoform because of frameshift mutation caused by 50 bp nucleotide deletion compared with MnSpz2. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis showed that MnSpz1, MnSpz2, and MnSpz3 were expressed in all the detected tissues of M. nipponense, and MnSpz2 was found to be the major isoform in the heart, gills, stomach, and intestine. After stimulation by Vibrio parahaemolyticus, Staphylococcus aureus, or White spot syndrome virus (WSSV), the expression levels of MnSpz1, MnSpz2, and MnSpz3 changed. Given the high similarities among MnSpz1-3, RNA interference (RNAi) using dsRNA of MnSpz1 inhibited the expression of the three Spätzle genes (MnSpz1, MnSpz2 and MnSpz3). Silencing of MnSpz1-3 down-regulated the expression levels of nine antimicrobial peptide (AMP) genes in M. nipponense. After Knockdown of MnSpzs, the number of V. parahaemolyticus, S. aureus and WSSV copies in M. nipponense increased significantly in vivo. Our results suggest that Spätzles are involved in the innate immunity of M. nipponense. The expansion of MnSpz genes through gene duplication is beneficial to enhance the innate immune defense ability of M. nipponense.
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http://dx.doi.org/10.1016/j.dci.2021.104254DOI Listing
January 2022

Exploring the sulfate patterns of chondroitin sulfate/dermatan sulfate and keratan sulfate in human pancreatic cancer.

J Pharm Biomed Anal 2021 Oct 26;205:114339. Epub 2021 Aug 26.

Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China. Electronic address:

This study was designed to explore the sulfation patterns of chondroitin sulfate (CS)/dermatan sulfate (DS), and keratan sulfate (KS) and the expression of carbohydrate sulfotransferases (CHSTs) in 26 pancreatic tumor and normal tissues. CS/DS and KS profiles were simultaneously determined. Pancreatic tumor tissues exhibited increased ΔDi-0S, ΔDi-4S, and ΔDi-6S levels, with absolute ΔDi-4S content being highest, followed by ΔDi-6S. However, as for the contents of KS-6S and KS-6S,6'S, there were no significant regular change. The expression levels of CHST1 and CHST4 were 37 and 15 times higher than those in normal tissues. PCA and OPLS-DA revealed that ΔDi-4S and ΔDi-6S levels could be reliably used to differentiate between healthy and cancerous tissues. The up-regulation of CHST3, CHST12, CHST13, and CHST15 was directly correlated with C-4 and C-6 sulfation. These data provide a foundation for future studies of the role of ΔDi-4S and ΔDi-6S in the progression of pancreatic cancer.
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http://dx.doi.org/10.1016/j.jpba.2021.114339DOI Listing
October 2021

High Neutrophil-To-Lymphocyte Ratio Is an Independent Risk Factor for End Stage Renal Diseases in IgA Nephropathy.

Front Immunol 2021 12;12:700224. Epub 2021 Aug 12.

West China School of Medicine, Sichuan University, Chengdu, China.

Background: Complex factors are involved in the development and progression of immunoglobulin A nephropathy (IgAN), a common primary glomerulonephritis worldwide. Autoimmunity and inflammation have been considered to be the basic mechanisms; however, the exact pathogenesis remains unclear. As a novel marker of inflammation, the neutrophil-to-lymphocyte ratio (NLR) has been studied in various diseases. Whether the NLR can predict the renal outcome of patients with IgAN remains unclear. We evaluated the relationships between the NLR and renal function, pathologic lesions, renal progression, and prognosis in patients with IgAN.

Methods: This retrospective study involved 966 patients with biopsy-proven IgAN. They were divided into two groups based on the cut-off value of the NLR: the high group (NLR ≥ 2.67, n = 384) and the low group (NLR < 2.67, n = 582). The endpoint was end-stage renal disease [estimated glomerular filtration rate (eGFR) of <15 mL/min/1.73 m or performance of renal replacement therapy]. A correlation test was conducted to explore the relationship between the NLR and other important parameters (eGFR, serum creatinine, proteinuria, hypertension and renal pathologic lesions). The predictive value was determined by the area under the receiver operating characteristics curve (AUROC). Kaplan-Meier and Cox proportional hazards analyses were performed to evaluate renal progression and prognosis.

Results: The NLR had the highest AUROC, which was 0.633 (p < 0.001). The correlation test revealed that the NLR was positively correlated with serum creatinine (r = 0.127, p < 0.001) and 24-hour urine protein (r = 0.18, p < 0.001) and negatively correlated with eGFR (r = 0.14, p < 0.001). Patients with IgAN who had a high NLR were more likely to have hypertension (p = 0.003). Multivariate Cox regression analysis indicated that a high NLR was an independent risk factor for IgAN even after adjustment for important clinical and pathological parameters (p = 0.043, HR = 1.74, 95%CI: 1.02-2.97). Kaplan-Meier analysis showed that a high NLR was significantly associated with the renal prognosis of patients with IgAN (p < 0.001), especially patients with stage 3 to 4 chronic kidney disease (p = 0.028) or 24-hour urine protein of >1 g/day (p < 0.001).

Conclusion: An elevated NLR affects the renal progression and prognosis in patients with IgAN and could be a marker for evaluation of renal function and pathologic lesions.
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http://dx.doi.org/10.3389/fimmu.2021.700224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387559PMC
August 2021
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