Publications by authors named "Wei Ma"

1,128 Publications

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miR-26a Reverses Multidrug Resistance in Osteosarcoma by Targeting MCL1.

Authors:
Ming Li Wei Ma

Front Cell Dev Biol 2021 18;9:645381. Epub 2021 Mar 18.

Department of Orthopaedic, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

The multidrug resistance (MDR) acquired in human osteosarcoma is a huge obstacle for effective chemotherapy. Recently, microRNA-26a (miR-26a) has been associated with the pathogenesis and progression of osteosarcoma. However, whether it regulates MDR in osteosarcoma is unknown. We show here that miR-26a expression declines in chemoresistant osteosarcoma after neoadjuvant chemotherapy, and its expression correlates with clinical outcome. In addition, compared with sensitive parental cells, miR-26a expression also declines in osteosarcoma MDR cells, together suggesting a negative correlation between miR-26a expression and MDR development in osteosarcoma. We also show that the enforced expression of miR-26a reverses MDR in osteosarcoma cells, and conversely, miR-26a knockdown confers MDR in chemosensitive osteosarcoma cells treated with doxorubicin, methotrexate, or cisplatin. Mechanistically, miR-26a directly targets the pro-survival protein myeloid cell leukemia 1 (MCL1), and in turn, the enforced expression of MCL1 markedly antagonizes miR-26a-decreased MDR in osteosarcoma MDR cells, therefore demonstrating that miR-26a reverses MDR in osteosarcoma by targeting MCL1. Lastly, miR-26a reverses resistance to doxorubicin in osteosarcoma MDR cells xenografted in nude mice. Collectively, these results reveal a negative role and the underlying mechanism of miR-26a in the regulation of MDR in human osteosarcoma, implying a potential tactic of manipulating miR-26a for overcoming MDR in osteosarcoma chemotherapy.
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http://dx.doi.org/10.3389/fcell.2021.645381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012539PMC
March 2021

The characteristics of carotid body tumors in high-altitude region: Analysis from a single center.

Vascular 2021 Apr 4:17085381211005238. Epub 2021 Apr 4.

Department of Cardiovascular Surgery, Qinghai University Affiliated Hospital, Xi'ning, China.

Objectives: Though carotid body tumors are rare, increasing attentions have been given because of malignant transformation and high surgical risk. However, at present, the characteristics and etiology still remain unclear. Our study was designed to describe the clinical features of carotid body tumors in our institution and to compare the results with previous reports.

Methods: We retrospectively reviewed carotid body tumor patients diagnosed in our institution from January 2015 to May 2020. The demographics, comorbidities, lesion location, anatomic characteristics, complications, and postoperative outcomes were evaluated. Carotid body tumor measurements were determined from computed tomography, magnetic resonance imaging, and carotid arteriography examination. We described and compared the clinical features of carotid body tumors in our institution and other reports.

Results: We totally identified 122 carotid body tumor cases for the present analysis. The mean age was 50.26 years, with the majority being female (82%). The commonest presentation was a painless neck mass (68%). For the distribution of nationality, most patients were the Han nationality (69.7%). The mean altitude of habitat of patients was 2689.4 km; 19.7% patients suffered bilateral lesions. The main blood supply of carotid body tumors was from external carotid artery (54.1%). For patients who received operation, 11 (11.2%) patients experienced cranial nerve injury. The maximal diameter of tumors was 3.99 ± 1.98 cm in male and 3.38 ± 1.36 cm in female. The volume of tumors was 31.49 ± 29.76 cm in male and 15.27 ± 13.06 cm in female. The distance to base of skull of tumors was 3.39 ± 1.07 cm (3.99 ± 1.98 cm in male vs 3.38 ± 1.36 cm, <0.05). Two patients (2.04%) were identified as having malignant carotid body tumor.

Conclusions: Though carotid body tumor had a low morbidity and multitudinous clinicopathologic features, it was apt to middle-aged women and the main blood supply was from external carotid artery. The painless neck mass was the commonest presentation of carotid body tumors. There were significant difference between male and female patients regarding platelet, hemoglobin, distance to base of skull, tumor volume, altitude of habitat, carotid body tumor location, and hypertension.
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http://dx.doi.org/10.1177/17085381211005238DOI Listing
April 2021

Genetic Mapping and Identification of the Candidate Gene for White Seed Coat in .

Int J Mol Sci 2021 Mar 15;22(6). Epub 2021 Mar 15.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Seed coat color is an important agronomic trait of edible seed pumpkin in . In this study, the development pattern of seed coat was detected in yellow and white seed coat accessions Wuminglv and Agol. Genetic analysis suggested that a single recessive gene () is involved in seed coat color regulation in . An F segregating population including 2798 plants was used for fine mapping and a candidate region containing nine genes was identified. Analysis of 54 inbred accessions revealed four main Insertion/Deletion sites in the promoter of encoding an MYB transcription factor were co-segregated with the phenotype of seed coat color. RNA-seq analysis and qRT-PCR revealed that some genes involved in phenylpropanoid/flavonoid metabolism pathway displayed remarkable distinction in Wuminglv and Agol during the seed coat development. The flanking InDel marker S1548 was developed to predict the seed coat color in the MAS breeding with an accuracy of 100%. The results may provide valuable information for further studies in seed coat color formation and structure development in Cucurbitaceae crops and help the molecular breeding of .
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http://dx.doi.org/10.3390/ijms22062972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000038PMC
March 2021

Clinical application of liver stiffness measurement in patients with cavernous transformation of portal vein.

Exp Ther Med 2021 May 28;21(5):442. Epub 2021 Feb 28.

Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.

The clinical outcomes differ between patients with cavernous transformation of the portal vein (CTPV) with and without cirrhosis. Therefore, invasive liver biopsy may be needed for the differential diagnosis of patients with CTPV with or without cirrhosis. The present study aimed to investigate the diagnostic efficacy of liver stiffness measurements (LSM) for the prediction of cirrhosis in patients with CTPV. A total of 20 patients with CTPV, 34 with chronic hepatitis B (CHB)-related cirrhosis and 20 healthy volunteers, were retrospectively recruited in the study. CTPV was diagnosed with contrast-enhanced computed tomography (CT) and ultrasound for the abdomen. LSM values were detected for each patient, while liver biopsy was performed in each patient in the CTPV and cirrhosis groups. The results demonstrated that LSM values were significantly lower in the CTPV group (12.5 kPa; range, 6.8-21.5 kPa) compared with the CHB-related cirrhosis group (21.0 kPa; range, 15.5-27.2 kPa; P=0.017). However, this was still higher compared with healthy volunteers (4.9 kPa; range 4.0-5.8 kPa; P<0.001). In addition, CTPV patients with cirrhosis (17.7 kPa; range, 13.9-30.8 kPa) exhibited significantly increased LSM values compared with those without cirrhosis (6.4 kPa; range, 5.7-7.8 kPa; P<0.001). Furthermore, LSM values in CTPV patients without cirrhosis were slightly higher compared with those of healthy volunteers (P=0.003), while no statistically significant difference was observed in LSM between CTPV patients with cirrhosis and CHB-related cirrhosis group. These findings indicated that LSM values could be used for the differential diagnosis of CTPV patients with or without cirrhosis. However, further validation studies are needed.
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http://dx.doi.org/10.3892/etm.2021.9881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967803PMC
May 2021

The mediated effect of HIV risk perception in the relationship between peer education and HIV testing uptake among three key populations in China.

AIDS Res Ther 2021 Mar 25;18(1). Epub 2021 Mar 25.

Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, 44 West Wenhua Road, Jinan, Shandong, 250012, People's Republic of China.

Background: Peer education and HIV risk perception are related to HIV testing uptake among key populations. We aimed to examine the association between peer education, HIV risk perception, and HIV testing uptake, as well as to evaluate the mediated effect of HIV risk perception in the relationship between peer education and HIV testing uptake.

Methods: We conducted a cross-sectional survey among 1188 HIV-uninfected or unknown participants from populations of men who have sex with men (MSM), female sex workers (FSWs), and drug users (DUs) in seven cities of China. Partial correlation analysis and regression analysis were employed to examine the associations among peer education, HIV risk perception, and HIV testing uptake. Mediation analysis was conducted to assess whether HIV risk perception mediated the hypothesized association.

Results: Receiving peer education was associated with higher odds of HIV testing uptake among MSM, FSWs and DUs. Perceiving risk of HIV infection was associated with higher odds of HIV testing uptake among MSM and DUs. Among MSM, the relationship between peer education and HIV testing uptake was mediated by moderate risk perception of HIV (indirect effect: 0.53, 95% CI 0.07 to 1.21), and by high risk perception of HIV (indirect effect: 0.50, 95% CI 0.01 to 1.17). Among DUs, the relationship between peer education and HIV testing uptake was mediated by moderate risk perception of HIV (indirect effect: 1.80, 95% CI 0.57 to 3.45).

Conclusions: Participants who received peer education tended to perceive their risk of HIV infection, which in turn was associated with increased HIV testing uptake among MSM and DUs. Therefore, in addition to peer education interventions, more report about HIV epidemic and risk assessment should also be scaled up to enhance HIV risk perception among key populations.
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http://dx.doi.org/10.1186/s12981-021-00334-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992327PMC
March 2021

Relation between Blood Pressure Variability within a Single Visit and Stroke.

Int J Hypertens 2021 2;2021:2920140. Epub 2021 Mar 2.

Division of Cardiology, Peking University First Hospital, Dahongluochang Street, Xicheng District, Beijing 100034, China.

Blood pressure variability (BPV) has been identified as an important risk factor for cardiovascular events. The white coat effect (WCE), which is measured as the first systolic blood pressure (SBP) measurement minus the mean of the second and third measurements, is a BPV indicator within a single visit. In total, 2,972 participants who had three measurements of BP within a single visit were included. The participants were divided into three groups based on their WCE percentiles: Group 1 (WCE, 2.5-97.5th percentiles of WCE), Group 2 (WCE, 0-2.4th percentiles of WCE), and Group 3 (WCE, 97.6-100th percentiles of WCE). A multiple logistic regression model was used to analyze the relationship between WCE and stroke after adjusting for cardiovascular disease risk factors. Compared with the WCE group, the OR for stroke in the WCE group was 2.78 (95% CI: 1.22, 6.36, =0.015). After adjusting for cardiovascular factors, OR increased to 3.12 (95% CI: 1.22, 7.96, =0.017). The OR of WCE for stroke was 0.93 (95%CI: 0.87, 0.99, =0.036). BPV within a single visit is associated with stroke. The value and direction of the change may be important as well.
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http://dx.doi.org/10.1155/2021/2920140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943313PMC
March 2021

Insights on the mechanism of Fe doped ZnO for tightly-bound extracellular polymeric substances tribo-catalytic degradation: The role of hydration layers at the interface.

Chemosphere 2021 Mar 3;276:130170. Epub 2021 Mar 3.

Department of Chemistry, Dalian University of Technology, Dalian, 116024, PR China.

The control of interfacial microbial pollution is of great significance for water safety. Herein, the tribo-catalysis ability of zinc oxide (ZnO) has been investigated, which can realize the control of tightly-bound extracellular polymeric substances (T-EPS) in water under dark environment. The DFT calculation proves the Fe doping introduces the impurity level and decreases the work function from 5.071 eV to 5.045 eV, improves the charge separation of ZnO, and eventually enhances the catalytic reaction efficiency. Characterizing the catalytic reaction process by three-dimensional fluorescence (3D EEM) and fluorescence regional integration (FRI) method, it is found that the T-EPS solution can be degraded 75.8% by Fe-ZnO in 12 min, while ZnO can only degrade 32.2%. Combining with high-resolution scanning probe microscope (HR-SPM) and attenuated total reflection method (ATR-FTIR), hydration layers consist with hydroxyl layer (∼0.23 nm) and water molecular layer (∼0.27 nm) are observed at the interface between Fe-ZnO and T-EPS solution, and terminal hydroxyl group (OH) is considered to be the active site for the generation of radicals. This study provides an idea for exploring the mechanism of tribo-catalytic reaction and shows its application prospect in the field of microbial inhibition in water.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130170DOI Listing
March 2021

Sample size re-estimation for covariate-adaptive randomized clinical trials.

Stat Med 2021 Mar 17. Epub 2021 Mar 17.

Department of Statistics, George Washington University, Washington, DC, USA.

Covariate-adaptive randomization (CAR) procedures have been developed in clinical trials to mitigate the imbalance of treatments among covariates. In recent years, an increasing number of trials have started to use CAR for the advantages in statistical efficiency and enhancing credibility. At the same time, sample size re-estimation (SSR) has become a common technique in industry to reduce time and cost while maintaining a good probability of success. Despite the widespread popularity of combining CAR designs with SSR, few researchers have investigated this combination theoretically. More importantly, the existing statistical inference must be adjusted to protect the desired type I error rate when a model that omits some covariates is used. In this article, we give a framework for the application of SSR in CAR trials and study the underlying theoretical properties. We give the adjusted test statistic and derive the sample size calculation formula under the CAR setting. We can tackle the difficulties caused by the adaptive features in CAR and prove the asymptotic independence between stages. Numerical studies are conducted under multiple parameter settings and scenarios that are commonly encountered in practice. The results show that all advantages of CAR and SSR can be preserved and further improved in terms of power and sample size.
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http://dx.doi.org/10.1002/sim.8939DOI Listing
March 2021

Stroke risk in arthritis: A systematic review and meta-analysis of cohort studies.

PLoS One 2021 16;16(3):e0248564. Epub 2021 Mar 16.

Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan, China.

Background And Objective: Stroke is a major contributor to the global burden of disease. Although numerous modifiable risk factors (RF) for stroke have been identified, some remain unexplained. Increasing studies have investigated stroke risk in arthritis, but their results are inconsistent. We aimed to synthesize, quantify, and compare the risk of stroke for the major types of arthritis in cohort studies by using a systematic review and meta-analysis approach.

Methods: We searched Chinese and English databases to identify relevant studies from inception to April 30, 2020. Only studies adjusting at least for age and sex were included. We calculated pooled effect estimates for relative risk (RR) and 95% confidence interval (CI) and identified potential sources of heterogeneity and publication bias.

Results: A total of 1,348 articles were retrieved, and after an preliminary screening of titles and abstracts, 69 were reviewed for full text, and finally, 32 met the criteria for meta-analysis. Stroke risk in arthritis was significantly increased in studies adjusting for age and sex (RR = 1.36, 95% CI: 1.27-1.46) and for at least one traditional risk factor (RR = 1.40, 95% CI: 1.28-1.54). The results of studies stratified by stroke subtype were consistent with the main finding (ischemic stroke: RR = 1.53, 95% CI: 1.32-1.78; hemorrhagic stroke: RR = 1.45, 95% CI: 1.15-1.84). In subgroup analysis by arthritis type, stroke risk was significantly increased in rheumatoid arthritis (RR = 1.38, 95% CI: 1.29-1.48), ankylosing spondylitis (RR = 1.49, 95% CI: 1.25-1.77), psoriatic arthritis (RR = 1.33, 95% CI: 1.22-1.45), and gout (RR = 1.40, 95% CI: 1.13-1.73) but not osteoarthritis (RR = 1.03, 95% CI: 0.91-1.16). Age and sex subgroup analyses indicated that stroke risk was similar by sex (women: RR = 1.47, 95% CI: 1.31-1.66; men: RR = 1.44, 95% CI: 1.28-1.61); risk was higher with younger age (<45 years) (RR = 1.46, 95% CI: 1.17-1.82) than older age (≥65 years) (RR = 1.17, 95% CI: 1.08-1.26).

Conclusions: Stroke risk was increased in multiple arthritis and similar between ischemic and hemorrhagic stroke. Young patients with arthritis had the highest risk.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248564PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7963101PMC
March 2021

Intermolecular Interaction Control Enables Co-optimization of Efficiency, Deformability, Mechanical and Thermal Stability of Stretchable Organic Solar Cells.

Small 2021 Mar 14:e2007011. Epub 2021 Mar 14.

State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University, Xi'an, 710049, China.

Promoting efficiency, deformability, and life expectancy of stretchable organic solar cells (OSCs) have always been key concerns that researchers are committed to solving. However, how to improve them simultaneously remains challenging, as morphology parameters, such as ordered molecular arrangement, beneficial for highly efficient devices actually limits mechanical stability and deformability. In this study, the unfavorable trade-off among these properties has been reconciled in an all-polymer model system utilizing a mechanically deformable guest component. The success of this strategy stems from introducing a highly ductile component without compromising the pristine optimized morphology. Preferable interaction between two donors can maintain the fiber-like structure while enhancing the photocurrent to improve efficiency. Morphology evolution detected via grazing incidence X-ray scattering and in situ UV-vis absorption spectra during stretching have verified the critical role of strengthened interaction on stabilizing morphology against external forces. The strengthened interaction also benefits thermal stability, enabling the ternary films with small efficiency degradation after heating 1500 h under 80 °C. This work highlights the effect of morphology evolution on mechanical stability and provides new insights from the view of intermolecular interaction to fabricate highly efficient, stable, and stretchable/wearable OSCs.
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http://dx.doi.org/10.1002/smll.202007011DOI Listing
March 2021

Nonintrusively Adjusting Structural Colors of Sealed Two-Dimensional Photonic Crystals: Immediate Transformation between Transparency and Intense Iridescence and Their Applications.

ACS Appl Mater Interfaces 2021 Mar 10;13(11):13861-13871. Epub 2021 Mar 10.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116023, P. R. China.

Responsive photonic crystals (PCs), which can adjust structural colors in response to external stimuli, show great potential applications in displays, sensors, wearable electronics, encryption, and anticounterfeiting. In contrast, conventional structure-intrusive adjustment manners that external stimuli directly interact with the ordered arrays may lead to structural damage or longer response time. Here, a noninvasive adjustment of the structural colors of two-dimensional (2D) PCs (2D-PCs) is explored based upon diffraction theory. Sealed 2D-PCs and 2D inverse opal photonic crystal (IOPC) flexible devices are prepared. They are highly transparent in air but immediately exhibit intense viewing angle-dependent structural colors after being dipped in water. The mechanism of transparent-iridescent immediate transformation is explained by Bragg's law. The design mechanism is examined by numerical simulation and spectral shifts in different external media. We demonstrate its applications in the fields of information encryption and anticounterfeiting by using the transparent-iridescent immediate transformation of sealed 2D-PC patterns and 2D IOPC free-standing films sealed on the product surface. Because of the strong contrast between transparency and intense iridescence, reversible and immediate transformation, and durability, sealed 2D-PCs and 2D IOPC flexible devices designed by the noninvasive adjustment strategy will lead to a variety of new applications in displays, sensors, wearable electronics, encryption, and anticounterfeiting.
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http://dx.doi.org/10.1021/acsami.1c02083DOI Listing
March 2021

Aptamer-Gated Ion Channel for Ultrasensitive Mucin 1 Detection.

Anal Chem 2021 03 10;93(11):4825-4831. Epub 2021 Mar 10.

International Joint Research Laboratory for Biointerface and Biodetection, State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.

Detection of cancer markers is important for early diagnosis and timely treatment of cancer. In this study, we fabricated a tailorable gold nanofilm-anodized aluminum oxide (Au-AAO) ion channel through nanoparticle self-assembly and proposed a highly sensitive and selective Mucin 1 (MUC1) detection method. By engineering the optimal layers of the Au-AAO ion channel and encoding the aptamer between the interlayers, a highly controllable ion rectification phenomenon was observed. From this, the relationship between the rectification ratio (RR) and the concentration of MUC1 was established and the highly sensitive detection of MUC1 is achieved. We found that the aptamer-modified Au-AAO ion channel has a good linear range within the MUC1 concentration of 1-10 fg mL and the limit of detection (LOD) was as low as 0.0364 fg mL (0.0025 aM). Thus, this research opens a new horizon for fabricating multi-functional ion channels as well as developing ultrasensitive detection technologies.
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http://dx.doi.org/10.1021/acs.analchem.0c04137DOI Listing
March 2021

A meta-analysis on the role of pre-existing chronic disease in the cardiac complications of SARS-CoV-2 infection.

iScience 2021 Apr 5;24(4):102264. Epub 2021 Mar 5.

School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been associated with multiple direct and indirect cardiovascular complications. We sought to analyze the association of host co-morbidities (chronic respiratory illnesses, cardiovascular disease [CVD], hypertension or diabetes mellitus [DM]) with the acute cardiovascular complications associated with SARS-CoV-2 infection. Individual analyses of the majority of studies found median age was higher by ~10 years in patients with cardiovascular complications. Pooled analyses showed development of SARS-CoV-2 cardiovascular complications was significantly increased in patients with chronic respiratory illness (odds ratio (OR): 1.67 [1.48, 1.88]), CVD (OR: 3.37 [2.57, 4.43]), hypertension (OR: 2.68 [2.11, 3.41]), DM (OR: 1.60 [1.31, 1.95]) and male sex (OR: 1.31 [1.21, 1.42]), findings that were mostly conserved during sub-analysis of studies stratified into global geographic regions. Age, chronic respiratory illness, CVD, hypertension, DM, and male sex may represent prognostic factors for the development of cardiovascular complications in COVID-19 disease, highlighting the need for a multidisciplinary approach to chronic disease patient management.
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http://dx.doi.org/10.1016/j.isci.2021.102264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934658PMC
April 2021

Outcomes of macular buckling surgery in myopic foveal detachment eyes with and without disrupted ellipsoid zone band: a case-control study.

Graefes Arch Clin Exp Ophthalmol 2021 Mar 6. Epub 2021 Mar 6.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China.

Purpose: To compare the outcomes of macular buckling (MB) surgery between myopic foveal detachment (FD) eyes with and without ellipsoid zone (EZ) disruption.

Methods: A retrospective, case-control study. Forty-four consecutive eyes from 44 patients received MB surgery for myopic FD between November 2017 and January 2019 were included. The eyes were divided into two groups according to the integrity of EZ on spectral-domain optical coherence tomography (SD-OCT): 28 eyes with disrupted EZ band and 16 eyes with intact EZ band. Main outcome measures were visual acuity and the duration of subfoveal fluid (SFF) after MB.

Results: The mean follow-up time was 17.64 ± 6.61 and 16.06 ± 5.78 months in the disrupted EZ and intact EZ group, respectively (P = 0.430). The logMAR best-corrected visual acuity (BCVA) improved significantly, from 1.13 ± 0.46 and 1.12 ± 0.39 at baseline to 0.85 ± 0.65 (P = 0.002) and 0.53 ± 0.33 (P = 0.000) for the disrupted EZ group and intact EZ group, respectively. The mean visual improvement was 15.00 ± 14.14 Early Treatment Diabetic Retinopathy Study (ETDRS) letters for the disrupted EZ group and 26.88 ± 19.48 ETDRS letters for the intact EZ group. Significant difference was found on both final postoperative BCVA (P = 0.035) and visual improvement (P = 0.025). At 6 months, SFF remained in 53.57% (15/28) of the eyes in the disrupted EZ group and in only 12.50% (2/16) of the eyes in the intact EZ group (P = 0.018).

Conclusion: The intact EZ group showed better functional and anatomical outcomes than the disrupted EZ group after MB surgery.
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http://dx.doi.org/10.1007/s00417-021-05123-1DOI Listing
March 2021

The application of amide units in the construction of neutral functional dyes for mitochondrial staining.

J Mater Chem B 2021 03;9(10):2524-2531

College of Chemistry, Chemical Engineering and Material Science, Soochow University, 199 Ren'Ai Road, Suzhou 215123, China. and Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.

To develop a new class of neutral fluorescent dyes with mitochondrial staining capacity, a series of functional dyes were obtained from Nile Red (2a-e) and coumarin (3a-e) with different amide compounds via Suzuki coupling reactions. The Nile Red derivatives (2a-e) emitted red fluorescence (590-660 nm) and coumarin derivatives (3a-e) showed blue emission (455-490 nm) in organic solvents. In addition, they exhibited high fluorescence quantum yields (0.27-0.98) in organic solvents and excellent photostability (>92%). Moreover, all of them possessed low cytotoxicity. More importantly, Nile Red borate (2) and coumarin borate (3) only accumulated in lipid droplets, while after being modified by different amide compounds, dyes 2a-e and 3a-e could successfully target mitochondria in HeLa cancer cells via confocal fluorescence experiments. This work provides a new strategy for the design of neutral cellular probes for mitochondrial staining.
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http://dx.doi.org/10.1039/d0tb02885aDOI Listing
March 2021

PIWI-interacting RNA sequencing profiles in maternal plasma-derived exosomes reveal novel non-invasive prenatal biomarkers for the early diagnosis of nonsyndromic cleft lip and palate.

EBioMedicine 2021 Mar 24;65:103253. Epub 2021 Feb 24.

Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, PR China. Electronic address:

Background: Congenital malformations are common birth defects with high neonatal morbidity and mortality. It is essential to find simpler and more efficient biomarkers for early prenatal diagnosis. Therefore, we investigated PIWI-interacting RNAs (piRNAs) as potential prenatal biomarkers in plasma-derived exosomes from pregnant women carrying foetuses with congenital malformations.

Methods: Small RNA sequencing was used to screen piRNA biomarkers in plasma-derived exosomes of five pregnant women carrying foetuses with nonsyndromic cleft lip and palate (nsCLP) and five women carrying normal foetuses. Differentially expressed piRNAs were verified in 270 pregnant women, including 111 paired women carrying foetuses with congenital malformations and normal foetuses (at 24 gestational weeks), 10 paired women carrying foetuses with nsCLP and normal foetuses (at 15-19 gestational weeks), and 28 women at different stages of normal pregnancy. piRNA biomarkers were also verified in placentas, umbilical cords, fetal medial calf muscles, and lip tissues of nsCLP and normal foetuses.

Findings: We identified a biomarker panel of three pregnancy-associated exosomal piRNAs (hsa-piR-009228, hsa-piR-016659, and hsa-piR-020496) could distinguish nsCLP foetuses from normal foetuses. These three piRNAs had better diagnostic accuracy for nsCLP at the early gestational stage, at which time typical malformations were not detected upon prenatal ultrasound screening, and had diagnostic value for neural tube defects (NTDs) and congenital heart defects (CHDs).

Interpretation: Our work revealed the potential clinical applications of piRNAs for predicting nsCLP, NTDs, and CHDs.

Funding: National Key Research and Development Program, National Natural Science Foundation of China, and LiaoNing Revitalization Talents Program .
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http://dx.doi.org/10.1016/j.ebiom.2021.103253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921467PMC
March 2021

Clinical Characteristics and CT Findings in 148 Non-COVID-19 Influenza-Like Illness Cases: A Retrospective Control Study.

Front Public Health 2021 9;9:616963. Epub 2021 Feb 9.

Department of Critical Care Medicine, The Sixth Medical Center, Chinese PLA General Hospital, Beijing, China.

This study was to collect clinical features and computed tomography (CT) findings of Influenza-Like Illness (ILI) cases, and to evaluate the correlation between clinical data and the abnormal chest CT in patients with the Influenza-Like Illness symptoms. Patients with the Influenza-Like Illness symptoms who attended the emergency department of The Six Medical Center of The PLA General Hospital from February 10 to April 1, 2020 were enrolled. Clinical and imaging data of the enrolled patients were collected and analyzed. The association between clinical characteristics and abnormal chest CT was also analyzed. A total of 148 cases were enrolled in this study. Abnormalities on chest CT were detected in 61/148 (41.2%) patients. The most common abnormal CT features were as follows: patchy consolidation 22/61(36.1%), ground-glass opacities 21/61(34.4%), multifocal consolidations 17/61(27.9%). The advanced age and underlying diseases were significantly associated with abnormal chest CT. Abnormal chest CT is a common condition in Influenza-Like Illness cases. The presence of advanced age and concurrent underlying diseases is significantly associated with abnormal chest CT findings in patients with ILI symptoms. The chest CT characteristic of ILI is different from the manifestation of COVID-19 infection, which is helpful for differential diagnosis.
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http://dx.doi.org/10.3389/fpubh.2021.616963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900189PMC
March 2021

Plantaginis Semen polysaccharides ameliorate renal damage through regulating NLRP3 inflammasome in gouty nephropathy rats.

Food Funct 2021 Mar 24;12(6):2543-2553. Epub 2021 Feb 24.

College of Pharmacy, Jiamusi University, Jiamusi, 154007, Heilongjiang, P.R. China.

Gouty nephropathy (GN) is considered to be a prevalent renal disease and is an inflammatory event mainly induced by MSU crystals. Plantaginis Semen is a traditional Chinese herb that has been used in the treatment of gout, gouty arthritis and GN, but the mechanism and ingredients have been unclear. In this study, we explored and evaluated the preliminary structural characterizations of Plantaginis Semen polysaccharides (PSPs) and the activity of protecting against renal damage in GN rats. Three polysaccharide fractions, PSP-D, PSP-H and PSP-S, were sequentially extracted by different processes from the seed of Plantago asiatica L. The Fourier transform infrared spectral (FTIR) results showed that there were significant differences between PSP-S and the other two polysaccharides (PSP-D and PSP-H). PSP-D and PSP-H have pyrene monomers and linkages of β-glycosides in their structures, and PSP-S has furanoside in the molecular structure. The scanning electron microscope (SEM) images of three polysaccharides showed that PSP-D has a smooth surface and a small curve, PSP-H is block-like and uneven in magnitude, whereas PSP-S is sea-tent-like and its surface is very distinct from the others. Main components and molar ratios are also different. Rats were randomly divided into six groups (n 8 per group): the control group, model group, positive group, and three treatment groups (PSP-D, PSP-H and PSP-S). For all groups except the control group, rats were intragastrically administered the adenine suspension (50 mg kg d) and fed with a high-yeast diet (15 g kg d) for 28 days. On the 9th day, the control group and the model group were administered normal saline at the same time. Treatment groups were individually given corresponding drugs for 20 days. We found that PSPs could prevent renal damage, including decreasing the inflammatory response and regulating the (NOD)-like receptor protein 3 (NLRP3) protein in renal tissue. The underlying mechanism was related to NLRP3 inflammasome signal pathways, and it could take effect through the down-regulation of the protein expression levels of NLRP3, ASC and caspase-1 and inhibit the release of downstream inflammatory factors. PSPs are promising polysaccharides that could protect against renal injury through ameliorating renal inflammation in GN rats. Plantaginis Semen polysaccharides are potential functional food ingredients or pharmacological agents for treating GN in clinical practice.
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http://dx.doi.org/10.1039/d0fo03143gDOI Listing
March 2021

PTPN6 promotes chemosensitivity of colorectal cancer cells via inhibiting the SP1/MAPK signalling pathway.

Cell Biochem Funct 2020 Dec 7. Epub 2020 Dec 7.

Department of Pharmacology, Xiangnan University, Chenzhou, China.

The abnormal expression of protein tyrosine phosphatase nonreceptor type 6 (PTPN6) has been proved to be associated with the progression of colorectal cancer. However, its role in chemosensitivity and related molecular mechanism have not been clarified. It has been reported that PTPN6 was down-regulated in colorectal cancer cells compared with the normal colorectal cells. To evaluate the effects of PTPN6 on the proliferation and survival of colorectal cancer cells, PTPN6 was overexpressed in colorectal cancer cells in the present study. We found that cell proliferation and viability were both decreased after overexpression of PTPN6. The IC50 of 5-Fu against colorectal cells was also declined in PTPN6 transfected cells. And further, we verified that PTPN6 could down-regulate the expression of P-gp and MRP-1. Moreover, SP1 was the target protein of PTPN6 predicated by ChIPBase software and confirmed through Co-immunoprecipitation assay and it was negatively regulated by PTPN6. To further verify the effect of SP1 on chemoresistance, SP1 was overexpressed. SP1 overexpression enhanced the drug-resistance to 5-Fu and abrogated the effects of PTPN6 upregulation on 5-Fu resistance. All the above changes were associated with the down-regulation of proteins related to MAPK signalling pathway, such as phosphorylation of extracellular regulated protein kinases (ERK) and p38. In summary, PTPN6 promoted chemosensitivity of colorectal cancer cells by targeting SP1 and inhibiting the activation of MAPK signalling pathway. SIGNIFICANCE OF THE STUDY: It has been demonstrated that the abnormal expression of PTPN6 was related to the progression of colorectal cancer. However, the chemosensitivity of PTPN6 and its molecular mechanisms were still unclear. Here, we identified that PTPN6 was down-regulated in colorectal cancer cells. Moreover, PTPN6 overexpression not only reduced cell proliferation and viability, but decreased the resistance of colorectal cells to 5-Fu. In our research, we found that the SP1 was the target protein of PTPN6 and it was negatively regulated by PTPN6. In addition, SP1 could increase the resistance of colorectal cells to 5-Fu. Molecular mechanism studies have shown that PTPN6 promoted the chemosensitivity of colorectal cancer cells by inhibiting the activation of MAPK signalling pathway.
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http://dx.doi.org/10.1002/cbf.3604DOI Listing
December 2020

Overexpression of removes the transrepression of proapoptotic genes mediated by the CtBP1-p300-FOXO3a complex and increases the chemosensitivity in osteosarcoma cells.

J Cancer 2021 21;12(6):1826-1837. Epub 2021 Jan 21.

Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.

Decreased expression of proapoptotic genes can lead to the chemoresistenance in cancer therapy. Carboxyl-terminal binding protein 1 (CtBP1), a transcriptional corepressor with multiple oncogenic effects, has been previously identified to suppress the expression of two proapoptotic genes [ (BCL2 associated X) and (Bcl-2 interacting mediator of cell death)] by assembling a complex with the Forkhead box O3 (FOXO3a) transcription factor and the p300 histone acetyltransferase. However, the upstream regulatory signaling of the CtBP1-p300-FOXO3a complex is obscure, and the effects of changing this signaling on chemosensitivity in osteosarcoma are unknown. Herein, we discovered that the downregulation of (Homeodomain-interacting protein kinase 2) was essential for the function of the CtBP1-p300-FOXO3a complex. Downregulation of prevented the phosphorylation and subsequent degradation of CtBP1, thereby allowing the assembly of the CtBP1-p300-FOXO3a complex and suppression of the expression of proapoptotic genes, such as , , (Bcl-2 interacting killer) and / (Phorbol-12-myristate-13-acetate-induced protein 1). Overexpression of promoted the phosphorylation of CtBP1 and the degradation of CtBP1 by proteasomes, thereby preventing the formation of the CtBP1-p300-FOXO3a complex. The abolition of CtBP1 transrepression increased the expression of proapoptotic genes to induce apoptosis and increase chemosensitivity in osteosarcoma cells. Taken together, our and results revealed that overexpression of could remove the CtBP1-mediated transrepression of proapoptotic genes, indicating a new therapeutic option for the treatment of osteosarcoma.
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http://dx.doi.org/10.7150/jca.52115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890331PMC
January 2021

Genome-wide landscape of mRNAs, microRNAs, lncRNAs, and circRNAs in hemorrhagic shock-induced ALI/ARDS in rats.

J Trauma Acute Care Surg 2021 Feb 17. Epub 2021 Feb 17.

Trauma Medicine Center, Peking University People's Hospital; Key Laboratory of Trauma and Neural Regeneration (Peking University); National Center for Trauma medicine of China, Beijing 100044, China Department of Central Laboratory & Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China Basic Medical Research Center, the Sixth Medical Center of the General Hospital of the Chinese People's Liberation Army, Beijing 100048, China Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing 100191, China.

Background: Hemorrhagic shock (HS) can develop into multiple organ dysfunction syndrome (MODS), among which acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) usually lead to poor outcomes. The underlying molecular mechanisms of HS-induced ALI/ARDS remain unclear. This study sought to investigate gene expression profiles and predict ceRNA regulatory networks in an HS-induced ALI/ARDS preclinical model.

Methods: SD rats were subjected to a fixed volume of hemorrhage (HS, 40% estimated total blood volume) or not (sham) randomly. After 8 hours of observation, left lung tissue was harvested to evaluate lung injury. Right lung was collected for RNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed and the lncRNA/circRNA-miRNA-mRNA linkages were predicted using the ceRNA theory. Quantitative real-time polymerase chain reaction was used to validate the RNA sequencing findings.

Results: HS lungs showed noticeable ALI/ARDS features, and 437 mRNAs, 31 miRNAs, 734 lncRNAs, and 29 circRNAs were differentially expressed. In GO and KEGG pathway analyses, the differentially expressed transcripts were enriched in the following terms: the metabolic pathways, signal transduction pathways, necroptosis, DNA damage recognition and repair, inflammatory cell migration and chemotaxis, the NOD-like receptor signaling pathway, the JAK-STAT signaling pathway, the MAPK signaling pathway, PI3K-Akt signaling, etc. And this study identified lncRNA-miRNA-mRNA linkages with 12 lncRNAs, 5 miRNAs, 15 mRNAs, and circRNA-miRNA-mRNA linkages with 10 circRNAs, 16 miRNAs, 39 mRNAs. These networks might play important regulatory roles.

Conclusions: This is the first high-throughput analysis of gene expression profiles in HS-induced ALI/ARDS. It shows that metabolism, cell signaling, DNA damage and repair, and necroptosis related RNAs altered, and inflammatory response associated RNAs and pathways have pivotal roles in HS-induced ALI/ARDS progression. It also prompts some important RNAs and regulatory networks for future research.

Level Of Evidence: basic science paper.
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http://dx.doi.org/10.1097/TA.0000000000003119DOI Listing
February 2021

Synthesis and Electronic Properties of Diketopyrrolopyrrole-Based Polymers with and without Ring-Fusion.

Macromolecules 2021 Jan 11;54(2):970-980. Epub 2021 Jan 11.

Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-41296 Göteborg, Sweden.

Diketopyrrolopyrroles (DPP) have been recognized as a promising acceptor unit for construction of semiconducting donor-acceptor (D-A) polymers, which are typically flanked by spacers such as thiophene rings a carbon-carbon single bond formation. It may suffer from a decrease in the coplanarity of the molecules especially when bulky side chains are installed. In this work, the two N atoms in the DPP unit are further fused with C-3 of the two flanking thiophene rings, yielding a π-expanded, very planar fused-ring building block (DPPFu). A novel DPPFu-based D-A copolymer (PBDTT-DPPFu) was successfully synthesized, consisting of a benzo[1,2-:4,5-']dithiophene (BDTT) unit as a donor and a DPPFu unit as an acceptor. For comparison, the unfused DPP-based counterpart PBDTT-DPP was also synthesized. Two dodecyl alkyl chains were attached to thiophene rings of DPP moieties to ensure good solubility of the DPPFu-based polymer. The influence of the ring-fusion effect on their structure, photophysical properties, electronic properties, molecular packing, and charge transport properties is investigated. Ring-fusion enhances the intermolecular interactions of PBDTT-DPPFu polymer chains as indicated by density functional theory calculation and analysis of electrostatic potential and van der Waals potential and results in significantly improved molecular packing for both the in-plane and out-of-plane directions as suggested by X-ray measurements. Finally, we correlate the molecular packing to the device performance by fabricating field-effect transistors based on these two polymers. The charge carrier mobility of the ring-fused polymer PBDTT-DPPFu is significantly higher as compared to the PBDTT-DPP polymer without ring-fusion, although PBDTT-DPPFu exhibited a much lower number-average molecular weight of 17 kDa as compared to PBDTT-DPP with a molecular weight of 108 kDa. The results from our comparative study provide a robust way to increase the interchain interaction by ring-fusion-promoted coplanarity.
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http://dx.doi.org/10.1021/acs.macromol.0c02326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880567PMC
January 2021

Silver-amplified fluorescence immunoassay via aggregation-induced emission for detection of disease biomarker.

Talanta 2021 Apr 16;225:121963. Epub 2020 Dec 16.

School of Chemistry & Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China. Electronic address:

Development of simple, robust, and reliable detection strategy of disease biomarkers holds tremendous promise for early clinical diagnosis and prognosis of diseases. In this work, through combining a silver nanoparticle (AgNP) linked immunoassay and aggregation induced emission (AIE)-based fluorogenic Ag probe, we developed a silver-amplified fluorescence immunoassay for the detection of disease biomarkers. This method overcame the intrinsic limitations of enzymes as the dissolution of AgNPs generated numerous Ag, which could switch on the fluorogenic Ag probe driven by tetrazolate-Ag complexation. As a proof of concept, our method could be used for determining α-fetoprotein (AFP) with a linear relationship in concentrations ranging from 0.1 ng mL to 5 μg mL and a low limit of detection of 42 pg mL. Our method was successfully confirmed for the detection of AFP in real serum samples from hepatocellular carcinoma (HCC) patients, demonstrating the great potential for clinical diagnosis.
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http://dx.doi.org/10.1016/j.talanta.2020.121963DOI Listing
April 2021

Pan-Cancer Analysis Shows Mutations Modulate the Association of NOX4 with Genetic Programs of Cancer Progression and Clinical Outcome.

Antioxidants (Basel) 2021 Feb 4;10(2). Epub 2021 Feb 4.

Laboratory of Clinical Immunology and Microbiology, Molecular Defenses Section, National Institutes of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA.

Previously, we have shown TGF-β-induced NOX4 expression is involved in the epithelial-to-mesenchymal transition (EMT), a process critical for cancer metastasis, and that wild-type (WT) and mutant (Mut) p53 have divergent effects on TGF-β induction of NOX4: WT-p53 suppresses whereas Mut-p53 augments NOX4 mRNA and protein production in several tumor cell models. We sought to validate and extend our model by analyzing whole-exome data of primary tumor samples in The Cancer Genome Atlas (TCGA). We constructed a Pan-Cancer dataset from 23 tumor types and explored expression patterns in relation to EMT and patient survival. NOX4 mRNA levels increase as a function of cancer progression in several cancers and correlate with Mut-p53 mRNA and genes involved in programs of EMT, cellular adhesion, migration, and angiogenesis. Tumor macrophages appear to be a source of NOX2, whose association with genetic programs of cancer progression emulate that of NOX4. Notably, increased expression is linked to poorer survival in patients with Mut-, but better survival in patients with WT-. NOX4 is negatively associated with markers of apoptosis and positively with markers of proliferation in patients with Mut-, consistent with their poorer survival. These findings suggest that mutations could "switch" NOX4 from being protective and an indicator of good prognosis to deleterious by promoting programs favoring cancer progression.
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http://dx.doi.org/10.3390/antiox10020235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915715PMC
February 2021

Can Swimming Microalgal Cells be Vehicles for ZnO Nanoparticle Transportation and Thus Lead to Zn Diffusion?

Bull Environ Contam Toxicol 2021 Apr 4;106(4):637-646. Epub 2021 Feb 4.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, No. 388 Lumo Road, Wuhan, 430074, Hubei, People's Republic of China.

The concentration of eco-toxic zinc oxide nanoparticles (nZnO) in aquatic ecosystems is increasing, and an effective method for their removal is needed. We hypothesize that microalgal cells may act as nZnO vehicles-if the nZnO concentration does not affect their swimming ability-enabling Zn diffusion and sedimentation. We conducted experiments using flasks connected via a U-type vessel; the first flask contained nZnO suspensions and second flask contained artificial seawater, respectively. We added microalgae to the first flask and illuminated the second. The microalgae appeared to promote sedimentation. However, only a few microalgal cells passed via phototaxis into the second flask, so the detection of nZnO or Zn ions in the second flask was not possible. Therefore, to confirm whether the microalgae affect Zn transportation, a more accurate method to detect nZnO or Zn ions at very low concentrations is needed.
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http://dx.doi.org/10.1007/s00128-021-03116-8DOI Listing
April 2021

Corrigendum to "Prevalence of Corneal Astigmatism in Patients before Cataract Surgery in Western China".

J Ophthalmol 2021 16;2021:4318290. Epub 2021 Jan 16.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China.

[This corrects the article DOI: 10.1155/2020/5063789.].
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http://dx.doi.org/10.1155/2021/4318290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834833PMC
January 2021

Accommodation and binocular vision changes after wearing orthokeratology lens in 8- to 14-year-old myopic children.

Graefes Arch Clin Exp Ophthalmol 2021 Feb 2. Epub 2021 Feb 2.

Department of Optometry and Visual Science, West China Hospital, Sichuan University, No. 37, Guoxuexiang, Wuhou District, Chengdu, 610041, Sichuan, China.

Purpose: The study aimed to observe and analyze the dynamic accommodation and binocular vision changes in myopic children after they switched from spectacles to orthokeratology (ortho-k) lenses.

Methods: Thirty-six myopic children aged 8-14 years were enrolled in this prospective, self-controlled study from West China Hospital. General information was gathered, and examinations were performed at baseline and at 1, 3, 6, and 12 months after switching to ortho-k lenses. The examination included assessments of distance/near visual acuity, distance/near horizontal and vertical ocular alignment, distance/near horizontal vergence range, accommodative amplitude, monocular/binocular accommodative facility, accommodative response, positive and negative relative accommodation (PRA/NRA), accommodation convergence/accommodation (AC/A), stereopsis, and reading ability.

Results: After the children switched to ortho-k lenses, distance and near ocular alignment showed an exophoric shift (distance: p = 0.001, near: p = 0.002), and the horizontal vergence range decreased by different degrees (convergence: distance blur point (p = 0.002), distance break point (p = 0.005), near blur point (p = 0.011), near break point (p = 0.043); divergence: distance break point (p < 0.001), distance recover point (p < 0.001), near recover point (p = 0.005)). The stereopsis ability (p < 0.001), monocular/binocular accommodative facility (p < 0.001), and PRA (p = 0.010) increased. The accommodative lag (p < 0.001), accommodative amplitude (p < 0.001), and calculated and gradient AC/A (calculated: p = 0.001, gradient: p = 0.025) decreased. The adjusted horizontal and vertical reading times and their ratio decreased (all p < 0.001).

Conclusion: The subjects showed distance and near exophoric shifts after switching to ortho-k lenses; improvements in accommodative function, stereopsis, and ocular motility; and a decrease in the binocular horizontal vergence range. The relationships between these changes and the ortho-k myopic control effects require further investigation.
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http://dx.doi.org/10.1007/s00417-021-05106-2DOI Listing
February 2021

Modelling the impact of an HIV testing intervention on HIV transmission among men who have sex with men in China.

HIV Med 2021 Jan 28. Epub 2021 Jan 28.

MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.

Objectives: An intervention developed through participatory crowdsourcing methods increased HIV self-testing among men who have sex with men [MSM; relative risk (RR) = 1.89]. We estimated the long-term impact of this intervention on HIV transmission among MSM in four cities (Guangzhou, Shenzhen, Jinan and Qingdao).

Methods: A mathematical model of HIV transmission, testing and treatment among MSM in China was parameterized using city-level demographic and sexual behaviour data and calibrated to HIV prevalence, diagnosis and antiretroviral therapy (ART) coverage data. The model was used to project the HIV infections averted over 20 years (2016-2036) from the intervention to increase self-testing, compared with current testing rates.

Results: Running the intervention once would avert < 2.2% infections over 20 years. Repeating the intervention (RR = 1.89) annually would avert 6.4-10.7% of new infections, while further increases in the self-testing rate (hypothetical RR = 3) would avert 11.7-20.7% of new infections.

Conclusions: Repeated annual interventions would give a three- to seven-fold increase in long-term impact compared with a one-off intervention. Other interventions will be needed to more effectively reduce the HIV burden in this population.
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http://dx.doi.org/10.1111/hiv.13063DOI Listing
January 2021

Identification of rye B chromosome-associated peptides by mass spectrometry.

New Phytol 2021 Jan 27. Epub 2021 Jan 27.

Department of Biosystems, KU Leuven, Willem Decroylaan 42, 3000, Belgium.

lB chromosomes (Bs) are supernumerary dispensable components of the standard genome (A chromosomes, As) that have been found in many eukaryotes. So far, it is unkown whether the B-derived transcripts translate to proteins or if the host proteome is changed due to the presence of Bs. lA comparative mass spectrometry was performed using the protein samples isolated from shoots of rye plants with and without Bs. We aimed to identify B-associated peptides and analyzed the effects of Bs on the total proteome. lOur comparative proteome analysis demonstrates that the presence of rye Bs affects the total proteome including different biological function processes. 319 out of 16776 quantified features were found in at least three out of five +B plants but not in 0B plants. 31 out of 319 features were identified as B-associated peptide features. According to our data mining, one B-specific protein fragment showed similarity to a glycine-rich RNA binding protein which differed from its A-paralog by two amino acid insertions. lOur result represents a milestone in B chromosome research, since this is the first report to demonstrate the existence of Bs changing the proteome of the host.
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http://dx.doi.org/10.1111/nph.17238DOI Listing
January 2021

Examining potential effects of non-occupational post-exposure prophylaxis (nPEP) on sexual behaviors of Chinese men who have sex with men: a cross-sectional study.

BMC Public Health 2021 Jan 26;21(1):221. Epub 2021 Jan 26.

Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, 44 West Wenhua Road, Jinan, 250012, Shandong Province, China.

Background: In China, non-occupational post-exposure prophylaxis (nPEP) is not a conventional service yet and nPEP related studies are very few. Recently, China's 13th Five Year Action Plan for HIV/AIDS Containment and Prevention examines the feasibility of including nPEP as one of the national strategies for HIV prevention. However, there is a concern that nPEP use might exacerbate high-risk sexual activities. In order to facilitate a research-based policy making of routinizing nPEP services, the current study examined potential effects of nPEP use on condom use and number of sexual partners among Chinese men who have sex with men (MSM) .

Methods: A cross-sectional survey was conducted in two cities of China in November and December 2018. Descriptive analyses of participants' sociodemographic and behavioral characteristics were conducted using SPSS 24.0. Mplus 7.4 was used to conduct confirmatory factor analysis and structural equation modeling.

Results: The sample included 419 Chinese MSM with a mean age of 28.04 (SD = 9.71). Participants reported more positive anticipation of their own behaviors than other MSM's behaviors regarding condom use and number of sexual partners if nPEP were to be routinized in China. About 60% of participants reported discrepancies between anticipated individual and population behaviors as a potential result of routinization of nPEP services. Anticipated individual behavioral change was positively related to age and duration of residence in the current city, and negatively related to education. Anticipated population behavioral change was positively related to age. Anticipated behavioral discrepancy was positively related to being ethnic minority and never married.

Conclusions: These findings identify a high-risk subgroup of MSM, who reported they would use condoms less and/or have more sexual partners when nPEP becomes available. This subgroup of MSM might benefit from targeted health interventions. Moreover, there is a clear discrepancy between anticipated individual and population behavioral changes regarding future routinization of nPEP services, suggesting incorporating nPEP services as a means of community development for MSM.
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http://dx.doi.org/10.1186/s12889-021-10283-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839183PMC
January 2021