Publications by authors named "Wei Luo"

1,435 Publications

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Ovomucin may be the key protein involved in the early formation of egg-white thermal gel.

Food Chem 2021 Jul 15;366:130596. Epub 2021 Jul 15.

Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China. Electronic address:

Clarification of the mechanism of heat-induced gel formation by proteins under natural food systems could provide important references for the regulation of food texture. In the present study, the proteins involved in the early stage (heating at 72 °C for 8 min) of egg-white thermal gel (EWG) formation were studied quantitatively through comparative proteomic analysis. We discovered that the abundance of ovalbumin and ovomucoid increased significantly (p < 0.01), whereas that of ovotransferrin, lysozyme, ovomucin (mucin 5B and mucin 6) decreased significantly (p < 0.01), in the supernatant of EWG. If the initial interaction of egg white proteins was altered by ultrasonic pretreatment, the abundance of ovomucin and lysozyme in the supernatant of EWG increased, and was accompanied by the change from a solid gel to a fluid gel. Based on these results, we hypothesize that ovomucin has a key role in the formation and regulation of EWG properties.
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http://dx.doi.org/10.1016/j.foodchem.2021.130596DOI Listing
July 2021

Integrated global analysis reveals a vitamin E-vitamin K1 sub-network, downstream of COLD1, underlying rice chilling tolerance divergence.

Cell Rep 2021 Jul;36(3):109397

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address:

Rice, a staple food with tropical/subtropical origination, is susceptible to cold stress, one of the major constraints on its yield and distribution. Asian cultivated rice consists of two subspecies with diverged chilling tolerance to adapt to different environments. The mechanism underlying this divergence remains obscure with a few known factors, including membrane protein CHILLING-TOLERANCE DIVERGENCE 1 (COLD1). Here, we reveal a vitamin E-vitamin K1 sub-network responsible for chilling tolerance divergence through global analyses. Rice genome regions responsible for tolerance divergence are identified with chromosome segment substitution lines (CSSLs). Comparative transcriptomic and metabolomic analysis of chilling-tolerant CSSL4-1 and parent lines uncovered a vitamin E-vitamin K1 sub-network in chloroplast with tocopherol (vitamin E) mediating chloroplast-to-nucleus signaling. COLD1, located in the substitution segment in CSSL4-1, is confirmed as its upstream regulator by transgenic material analysis. Our work uncovers a pathway downstream of COLD1, through which rice modulates chilling tolerance for thermal adaptation, with potential utility in crop improvement.
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http://dx.doi.org/10.1016/j.celrep.2021.109397DOI Listing
July 2021

Curcumin attenuates Adriamycin-resistance of acute myeloid leukemia by inhibiting the lncRNA HOTAIR/miR-20a-5p/WT1 axis.

Lab Invest 2021 Jul 19. Epub 2021 Jul 19.

Department of Hematology, People's Hospital of Longhua District, Shenzhen, Guangdong Province, People's Republic of China.

Acute myeloid leukemia (AML) is a common subtype of leukemia, and a large proportion of patients with AML eventually develop drug resistance. Curcumin exerts cancer suppressive effects and increases sensitivity to chemotherapy in several diseases. This study aimed to investigate the mechanism by which curcumin affects the resistance of AML to Adriamycin by regulating HOX transcript antisense RNA (HOTAIR) expression. Cell viability, colony-formation, flow cytometry, and Transwell assays were used to assess cell proliferation, apoptosis, and migration. A dual-luciferase reporter assay was used to verify the interaction between microRNA (miR)-20a-5p and HOTAIR or Wilms' tumor 1 (WT1). RT-qPCR and Western blotting assays were performed to detect gene and protein expression. The results showed that curcumin suppressed the resistance to Adriamycin, inhibited the expression of HOTAIR and WT1, and promoted the expression of miR-20a-5p in human acute leukemia cells (HL-60) or Adriamycin-resistant HL-60 cells (HL-60/ADR). Furthermore, curcumin suppressed proliferation and promoted apoptosis of HL-60/ADR cells. Overexpression of HOTAIR reversed the regulatory effect of curcumin on apoptosis and migration and restored the effect of curcumin on inducing the expression of cleaved caspase3, Bax, and P27. In addition, HOTAIR upregulated WT1 expression by targeting miR-20a-5p, and inhibition of miR-20a-5p reversed the regulation of Adriamycin resistance by curcumin in AML cells. Finally, curcumin inhibited Adriamycin resistance by suppressing the HOTAIR/miR-20a-5p/WT1 pathway in vivo. In short, curcumin suppressed the proliferation and migration, blocked the cell cycle progression of AML cells, and sensitized AML cells to Adriamycin by regulating the HOTAIR/miR-20a-5p/WT1 axis. These findings suggest a potential role of curcumin and HOTAIR in AML treatment.
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http://dx.doi.org/10.1038/s41374-021-00640-3DOI Listing
July 2021

The Mechanical Properties of Early Aged Shotcrete under Internal Sulfate Attack.

Materials (Basel) 2021 Jul 2;14(13). Epub 2021 Jul 2.

South City Power Supply Branch of State Grid Chongqing Electric Power Company, Chongqing 404100, China.

Shotcrete is the primary material for tunnel support due to its early rapid hardening characteristics. During tunnel construction in a sulfate environment, the hardening law of concrete will be affected. In this study, samples were prepared at six different curing times and immersed in four different concentrations of sulfate solutions. A uniaxial test was conducted and analyzed to investigate the effect of sulfate attack on the mechanical properties of early aged shotcrete materials. Results indicated that waterlogged shotcrete does not have apparent cracks on the outside. The stress-strain curve or ultimate compressive strength of the samples showed that the effect of sulfate on shotcrete should be differentiated into chemical and physical sulfate attacks, according to the concentration of sulfate ions. The two parameters in the equation of the hardening behaviors of sulfate attack samples, ultimate compressive strength, and time constant, are related to sulfate concentration. The crack damage stress threshold of samples demonstrates that high-concentration sulfate corrosion leads to an impact on the durability of shotcrete.
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http://dx.doi.org/10.3390/ma14133726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269896PMC
July 2021

Programmed cell death protein-1 (PD-1) protects liver damage by suppressing IFN-γ expression in T cells in infants and neonatal mice.

BMC Pediatr 2021 Jul 16;21(1):317. Epub 2021 Jul 16.

Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangdong Provincial Children's Medical Research Center, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, Guangdong, China.

Background: Biliary atresia (BA) is a severe cholangiopathy possibly resulting from virus-induced and immune-mediated injury of the biliary system. IFN-γ, secreted from CD4 Th1 cells and CD8 cytotoxic T cells, is a major mediator of liver pathology. Programmed death protein-1 (PD-1) signaling suppresses T cell function. However, how PD-1 modify T cell function in BA remains incompletely understood.

Methods: Frequencies of PD-1 expressing CD4 and CD8 T cells were analyzed in the liver and blood from BA and control subjects. Associations of PD-1CD4/CD8T cell abundances with liver function indices were measured. Function of PD-1 was measured by administration of an anti-PD-1 antibody in a Rhesus Rotavirus (RRV)-induced BA model. Survival, histology, direct bilirubin, liver immune cell subsets and cytokine production were analyzed.

Results: PD-1 was significantly upregulated in CD4 and CD8 T cells in patients with BA compared with control subjects. PD-1 expression in T cells was negatively associated with IFN-γ concentration in liver (PD-1CD4T cells in liver vs. IFN-γ concentration, r = - 0.25, p = 0.05; PD-1CD8T cells in liver vs. IFN-γ concentration, r = - 0.39, p = 0.004). Blockade of PD-1 increased IFN-γ expression in CD4 T and CD8 T cells (RRV vs. anti-PD-1 treated RRV mice: 11.59 ± 3.43% vs. 21.26 ± 5.32% IFN-γ in hepatic CD4T cells, p = 0.0003; 9.33 ± 4.03% vs. 22.55 ± 7.47% IFN-γ in hepatic CD8T cells, p = 0.0001), suppressed bilirubin production (RRV vs. anti-PD-1 treated RRV mice: 285.4 ± 47.93 vs. 229.8 ± 45.86 μmol/L total bilirubin, p = 0.01) and exacerbated liver immunopathology.

Conclusions: PD-1 plays a protective role in infants with BA by suppressing IFN-γ production in T cells. Increasing PD-1 signaling may serve as a therapeutic strategy for BA.
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http://dx.doi.org/10.1186/s12887-021-02794-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284022PMC
July 2021

Pathogenic variants of meiotic double strand break (DSB) formation genes PRDM9 and ANKRD31 in premature ovarian insufficiency.

Genet Med 2021 Jul 13. Epub 2021 Jul 13.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Purpose: The etiology of premature ovarian insufficiency (POI) is heterogeneous, and genetic factors account for 20-25% of the patients. The primordial follicle pool is determined by the meiosis process, which is initiated by programmed DNA double strand breaks (DSB) and homologous recombination. The objective of the study is to explore the role of DSB formation genes in POI pathogenesis.

Methods: Variants in DSB formation genes were analyzed from a database of exome sequencing in 1,030 patients with POI. The pathogenic effects of the potentially causative variants were verified by further functional studies.

Results: Three pathogenic heterozygous variants in PRDM9 and two in ANKRD31 were identified in seven patients. Functional studies showed the variants in PRDM9 impaired its methyltransferase activity, and the ANKRD31 variations disturbed its interaction with another DSB formation factor REC114 by haploinsufficiency effect, indicating the pathogenic effects of the two genes on ovarian function were dosage dependent.

Conclusion: Our study identified pathogenic variants of PRDM9 and ANKRD31 in POI patients, shedding new light on the contribution of meiotic DSB formation genes in ovarian development, further expanding the genetic architecture of POI.
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http://dx.doi.org/10.1038/s41436-021-01266-yDOI Listing
July 2021

Discharge-Induced Enhancement of the Oxygen Evolution Reaction.

Angew Chem Int Ed Engl 2021 Jul 12. Epub 2021 Jul 12.

Jiangsu University, School of Chemistry and Chemical Engineering, Xuefu Road 301, 212013, Zhenjiang, CHINA.

The fundamental understanding of the surface reconstruction induced by the applied potential is of great significance for enhancing the oxygen evolution reaction (OER). Here, we show that a previously overlooked discharge current in the low applied potential region also leads to in situ electrochemical activation of a nitrogen-doped nickel oxyhydroxide surface. We exploit the fact that doping of heteroatoms weakens the surface structure, and hence, a weak discharge current originating from the capacitive nature of nickel oxyhydroxide has a strong structure-reforming ability to promote the formation of nitrogen and oxygen vacancies. The current density at 1.4 V (vs. Hg/HgO) can dramatically increase by as much as 31.3% after discharge in the low applied potential region. This work provides insight into in situ enhancement of the OER and suggests that the low applied potential region must be a primary consideration in evaluating the origin of the activity of electrocatalysts.
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http://dx.doi.org/10.1002/anie.202108770DOI Listing
July 2021

Dissociation of Subjective and Objective Alertness During Prolonged Wakefulness.

Nat Sci Sleep 2021 28;13:923-932. Epub 2021 Jun 28.

Key Laboratory of Brain, Cognition and Education Sciences (South China Normal University), Ministry of Education; Center for Sleep Research, Center for Studies of Psychological Application, Guangdong Key Laboratory of Mental Health and Cognitive Science, School of Psychology, South China Normal University, Guangzhou, 510631, People's Republic of China.

Background: Although the deterioration of subjective and objective alertness during prolonged wakefulness has been investigated rigorously, whether perceived sleepiness and fatigue are consistent with actual decrements in behavioral performance in the time course is still disputed. The present study examined the dissociation between decrements of subjective alertness and performance deficits during prolonged wakefulness of one night and explored the relationship between body temperature and the impairments of subjective and objective alertness.

Participants And Methods: Thirty-eight participants (27 females; age: 21.76 ± 2.37 years old) underwent prolonged wakefulness for one night at habitual bedtime (0:00-6:00 am). Participants completed a 10-min PVT to assess objective alertness, fatigue, and sleepiness ratings to assess subjective alertness every 2 hours, and body temperature was measured every hour during scheduled wakefulness.

Results: Subjective alertness reflected a linear decline with time, but the magnitudes of objective performance deterioration increased significantly between 4:00 and 6:00 am. The increasing magnitudes of performance deficits were associated with the change of body temperature between 4:00 and 6:00 am.

Conclusion: These results indicate that the perceived degree of decline in alertness is temporally dissociated with the actual decline in objective vigilance with increased duration of wakefulness. The dissociation of magnitudes of subjective and objective alertness decrements mainly occurs between 4:00 and 6:00 am, and the changes of performance deficits have a relationship with body temperature.
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http://dx.doi.org/10.2147/NSS.S312808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254410PMC
June 2021

Guaiane-type sesquiterpenoids from Cinnamomum migao H. W. Li: And their anti-inflammatory activities.

Phytochemistry 2021 Jul 1;190:112850. Epub 2021 Jul 1.

Department of Natural Product Chemistry, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, PR China; Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address:

The phytochemical assessment of Cinnamomum migao H. W. Li fruits illustrated the isolation and identification of ten undescribed guaiane-type sesquiterpenoids "miganoids A-J″ and one undescribed sesquiterpene "7(S)-(hydroxypropanyl)-3-methyl-2-(4-oxopentyl) cyclohex-2-en-1-one". The extensive analysis of HRESIMS, 1D NMR, 2D NMR, experimental circular dichroism (ECD), and calculated (ECD) analysis entirely corroborated the configuration and confirmation of these isolated compounds. Moreover, the anti-inflammatory properties of the reported compounds were established by determining the LPS induced nitric oxide production. In the current study, miganoid C is testified the most active compound with about 89% NO inhibition. Additionally, miganoids C, E, and G also exhibited moderate inhibitory effects against the pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6). The IC values for miganoid C and miganoid G were determined as 19.4 and 14.5 μΜ against TNF-α mRNA, respectively.
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http://dx.doi.org/10.1016/j.phytochem.2021.112850DOI Listing
July 2021

The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice.

Mol Plant 2021 Jun 30. Epub 2021 Jun 30.

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Innovation Academy for Seed Design, CAS, Beijing 100101, China. Electronic address:

G-protein signaling and ubiquitin-dependent degradation are both involved in grain development in rice, but how these pathways are coordinated in regulating this process is unknown. Here, we show that Chang Li Geng 1 (CLG1), which encodes an E3 ligase, regulates grain size by targeting the Gγ protein GS3, a negative regulator of grain length, for degradation. CLG1 overexpression led to increased grain length, and overexpression of mutated CLG1 with changes in three conserved amino acids decreased grain length. CLG1 physically interacted with and ubiquitinated GS3; the latter was subsequently degraded through the endosome degradation pathway, leading to increased grain size. We also identified a critical SNP in exon 3 of CLG1 that was significantly associated with grain-size variation in a core collection of cultivated rice. This SNP resulted in an amino acid substitution from Arg to Ser at position 163, which enhancing the E3 ligase activity of CLG1, thus increasing grain size. Both the CLG1 expression level and the SNP may be useful targets for manipulating grain size.
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http://dx.doi.org/10.1016/j.molp.2021.06.027DOI Listing
June 2021

Modulation of TRIB3 and Macrophage Phenotype to Attenuate Insulin Resistance After Downhill Running in Mice.

Front Physiol 2021 9;12:637432. Epub 2021 Jun 9.

Department of Sports and Health Sciences, Nanjing Sport Institute, Nanjing, China.

Eccentric exercise training accompanied by a low-fat diet can prevent insulin resistance (IR) and is currently an effective method for the treatment of IR induced by high-fat diet (HFD)-associated obesity. However, the molecular mechanisms underlying this improvement of IR in adipose tissue are still not completely clear. In this study, 5-6-week-old male mice were randomly divided into a standard control diet (SCD) group (SC, = 12) and a HFD group (HF, = 72). After 12 weeks, 12 mice in each group were randomly sacrificed. The remaining mice in the HF group were randomly submitted to one of the following experimental protocols for 8 weeks: obesity-HFD-sedentary (OHF-Sed, = 14), obesity-HFD-exercise (OHF-Ex, = 16), obesity-SCD-sedentary (OSC-Sed, = 14), and obesity-SCD-exercise (OSC-Ex, = 16). All obese mice in the exercise group were subjected to downhill running. Half of the mice in each group received an insulin injection (0.75 U/kg) before sample collection. Epididymal fat was removed and weighed. Adipocyte size and inflammatory cell infiltration were observed by H&E staining. Both basal and insulin-stimulated GLUT4 fluorescence and protein contents were detected by immunofluorescence and Western blot. Levels of IL-1β and IL-10 were detected by ELISA. Protein contents of iNOS, Arg-1, TRIB3, p-AKT, and AKT were determined by Western blot. CD86 and CD206 fluorescence were determined by immunofluorescence. The results showed that a HFD for 12 weeks induced IR accompanied by adipose tissue macrophages M1 polarization (increased iNOS protein content and CD86 fluorescence) and TRIB3-AKT activation. Downhill running accompanied by a low-fat diet attenuated IR ( < 0.01), reduced inflammation levels (increased IL-10 protein content and decreased IL-1β protein content), inhibited adipose tissue macrophages M1 polarization (decreased iNOS protein content and CD86 fluorescence) and promoted M2 polarization (increased Arg-1 protein content and CD206 fluorescence), and suppressed TRIB3-AKT signaling. We concluded that downhill running accompanied by dietary fat regulation attenuates HFD-related IR in mice, which may be associated with reduced TRIB3-AKT signaling and activated M2 macrophages in adipose tissue.
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http://dx.doi.org/10.3389/fphys.2021.637432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220223PMC
June 2021

Novel circular RNA circSLIT2 facilitates the aerobic glycolysis of pancreatic ductal adenocarcinoma via miR-510-5p/c-Myc/LDHA axis.

Cell Death Dis 2021 Jun 24;12(7):645. Epub 2021 Jun 24.

Department of Hepatobiliary Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

Increasing evidence has indicated the great diagnostic and therapeutic potentials of circular RNAs (circRNAs) in human cancers. Although the biological roles of circRNAs in pancreatic ductal adenocarcinoma (PDAC) have been partially annotated, the potential regulatory mechanism of circRNAs in PDAC tumorigenesis remains poorly understood. Here, our study found that the novel circRNA circSLIT2 was significantly upregulated in PDAC tissues and cells. Clinically, ectopic high-expression of circSLIT2 was correlated with unfavorable prognosis of PDAC patients. Functional experiments demonstrated that circSLIT2 promoted the aerobic glycolysis and proliferation of PDAC cells in vitro, and circSLIT2 knockdown inhibited tumor growth in vivo. Mechanistically, circSLIT2 acted as miRNA sponge to target miR-510-5p/c-Myc axis. Furthermore, c-Myc bound with the promoter region of lactate dehydrogenase A (LDHA) to activate the transcription. Collectively, present findings reveal that circSLIT2/miR-510-5p/c-Myc/LDHA axis participates in the aerobic glycolysis and carcinogenesis of PDAC, and may act as a promising therapeutic target.
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http://dx.doi.org/10.1038/s41419-021-03918-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225611PMC
June 2021

Predicting Cervical Cancer Outcomes: Statistics, Images, and Machine Learning.

Authors:
Wei Luo

Front Artif Intell 2021 7;4:627369. Epub 2021 Jun 7.

Department of Radiation Medicine, University of Kentucky, Lexington, KY, United States.

Cervical cancer is a very common and severe disease in women worldwide. Accurate prediction of its clinical outcomes will help adjust or optimize the treatment of cervical cancer and benefit the patients. Statistical models, various types of medical images, and machine learning have been used for outcome prediction and obtained promising results. Compared to conventional statistical models, machine learning has demonstrated advantages in dealing with the complexity in large-scale data and discovering prognostic factors. It has great potential in clinical application and improving cervical cancer management. However, the limitations of prediction studies and prediction models including simplification, insufficient data, overfitting and lack of interpretability, indicate that more work is needed to make clinical outcome prediction more accurate, more reliable, and more practical for clinical use.
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http://dx.doi.org/10.3389/frai.2021.627369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215338PMC
June 2021

A γ-glutamylcysteine ligase AcGCL alleviates cadmium-inhibited fructooligosaccharides metabolism by modulating glutathione level in Allium cepa L.

J Hazard Mater 2021 Jun 8;419:126255. Epub 2021 Jun 8.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, China; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Fructooligosaccharides (FOS) are important carbohydrates in plants. Cadmium (Cd) toxicity limits growth and development in several plant species. Whether FOS metabolism is affected by Cd and the molecular mechanisms of tolerance of the effects of Cd toxicity in plants remain enigmatic. In the present study, FOS metabolism was analyzed under Cd stress in onion (Allium cepa L.). Results showed that Cd stress can inhibit FOS accumulation in onion, followed by the upregulation of a putative onion γ-glutamylcysteine ligase gene AcGCL. Heterologous expression of the AcGCL protein in Escherichia coli revealed that this recombinant enzyme has GCL activity. Furthermore, overexpressing AcGCL significantly increased glutathione (GSH) accumulation in young onion roots under Cd treatment, accompanied by increased phytochelatin (PC) amount, and increased transcript expression of GSH synthetase (GS), and phytochelatin synthase (PCS) genes. Notably, compared with control, overexpressing AcGCL ameliorated Cd phytotoxicity on onion FOS metabolism, which correlated with increased FOS synthesis. Taken together, these results suggest that the function of AcGCL as a γ-glutamylcysteine ligase can alleviate Cd inhibited FOS metabolism by modulating GSH levels in onion.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126255DOI Listing
June 2021

A Scoring System for Outpatient Orthopedist to Preliminarily Distinguish Spinal Metastasis from Spinal Tuberculosis: A Retrospective Analysis of 141 Patients.

Dis Markers 2021 29;2021:6640254. Epub 2021 May 29.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Objective: Spinal tuberculosis (TB) misdiagnosed of spinal metastasis was not rarely reported, especially in outpatients department. This study was aimed to establish an outpatient scoring system to preliminarily distinguish spinal metastasis from spinal TB.

Methods: We retrospectively reviewed consecutive 141 patients with a pathological diagnosis of spinal metastasis (82 cases) or spinal TB (59 cases) in our hospital from January 2017 to June 2018. The following clinical characteristics which can be obtained by outpatient orthopedist were recorded and analyzed: age, gender, malignant tumor history, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and imaging features including distribution characteristics of vertebral lesions, subligamentous spread, paravertebral or psoas abscess, involved vertebral element, intervertebral disc, and sequestra formation. The prevalence of clinical characteristics in spinal metastasis was evaluated, and the scoring system was established using logistic regression analysis. The performance of the scoring system was also prospectively validated.

Results: The outpatient scoring system was based on five clinical characteristics confirmed as significant predictors of spinal metastasis, namely, malignant tumor history, subligamentous spread, posterior element lesions, preserved discs, and no sequestra formation. Spinal metastasis showed a significant higher score than spinal TB (8.17 points 1.97 points, = 18.621, < 0.001), and the optimal cut-off value for the scoring system was 5 points. The sensitivity and specificity of the scoring system for predicting spinal metastasis were 97.85% and 88.33%, respectively, in the validation set.

Conclusion: Spinal lesions with the score of 5 to 10 would be considered a diagnosis of spinal metastasis, while the score of 0 to 4 may be spinal TB. Because the scoring system is mainly based on the clinical characteristics that can be obtained by an outpatient orthopedist, it is suitable to be used as a diagnostic tool in the outpatient department.
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http://dx.doi.org/10.1155/2021/6640254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179772PMC
May 2021

Improving modeling of ecosystem gross primary productivity through re-optimizing temperature restrictions on photosynthesis.

Sci Total Environ 2021 Sep 17;788:147805. Epub 2021 May 17.

College of Resources and Environment Science, Hunan Normal University, Changsha, Hunan 410081, China. Electronic address:

The terrestrial ecosystem gross primary productivity (GPP) plays an important role in the global carbon cycle and ecosystem functions. However, the estimates of GPP still have large uncertainties due to insufficient understanding of the photosynthesis-temperature relationship and maximum light use efficiency (LUE). We used satellite-derived proxies of GPP to derive optimum, minimum, and maximum temperature for photosynthesis at the ecosystem scale, which was then used to construct a new temperature stress expression. This study improves the MODIS-based light use efficiency model through coupling the optimized LUE with the new proposed temperature stress expression. The new model (R = 0.81, RMSE = 17.8 gC m (16 d)) performed better than the MODIS GPP products (R = 0.67, RMSE = 30.4 gC m (16 d)), especially for evergreen broadleaf forests and croplands. The mean annual GPP over China is 5.7 ± 0.27 PgC, and the GPP significantly increased by 0.046 ± 0.006 PgC year during 2001-2018. This study provides a potential method for future projections of terrestrial ecosystem functioning.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147805DOI Listing
September 2021

C and L band room-temperature continuous-wave InP-based microdisk lasers grown on silicon.

Opt Lett 2021 Jun;46(12):2836-2839

Quantum-dot (QD) and quantum-dash (QDash) have been shown to be promising gain materials for lasers directly grown on Si due to their better tolerance to crystal defects and thermal stability. Here we report optically pumped InP-based InAs QDash microdisk lasers (MDLs) directly grown on on-axis (001) Si. To the best of our knowledge, this is the first demonstration of room-temperature continuous-wave lasing of a QDash MDL on Si in the C band and L band. To the best of our knowledge, the lowest threshold of around 400 µW and highest operation temperature of 323 K have been achieved. An analysis of experimental results shows that the dominant lasing wavelength of MDLs varies with the thickness and diameter of the MDLs. Our demonstration shows potential application of MDLs for multi-channel operation in densely integrated Si-photonics.
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http://dx.doi.org/10.1364/OL.420106DOI Listing
June 2021

Evaluation of efficacy and safety of combined rosuvastatin and atorvastatin in treating with coronary heart disease: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26340

Department of Hematology, the Affiliated Hospital of Qinghai University, Xining, Qinghai.

Background: Globally, coronary heart disease (CHD) is a primary cause of morbidity leading to disabilities and mortality. Modern clinical practice adopts several pharmacological methods to treat CHD. Angina pectoris refers to sever chest pain due to CHD, it has a profound impact on the wellbeing of patients. Moreover, angina pectoris is a crucial prognosis predictor. The aim of the current study is to evaluate the effectiveness and safeness of using combined rosuvastatin and atorvastatin to treat CHD patients.

Methods: A systematic literature search for articles will be conducted on several electronic databases from their inception to May 2021. The search will include all randomized controlled trials examining the use of rosuvastatin in combination with atorvastatin to treat CHD patients. The databases are as follows: MEDLINE, Web of Science, the Cochrane Library, WanFang database, China National Knowledge Infrastructure, and EMBASE. A couple of authors will independently assess the eligibility, extract study data, and assess the possibility of bias. Moreover, depending on the type of data and heterogeneity of the included studies, either the Mantel-Haensel fixed-effect model or the DerSimonian-Laird random-effect model will be used to estimate the relative risk, mean differences, or standardized mean differences and 95% confidence intervals. All differences in opinion shall be decided by involving an additional author in the discussion. Lastly, the RevMan software (version: 5.3) will be used to perform sensitivity analysis, data synthesis, and risk of bias assessment.

Results: The effectiveness and security of using rosuvastatin in combination with atorvastatin to treat CHD patients will be systematically evaluated.

Conclusion: This study will provide evidence to evaluate the efficacy and security of using a combination of rosuvastatin and atorvastatin to treat CHD patients.

Ethics And Dissemination: Ethical approval will not be required since it is based on already published data.

Registration Number: DOI 10.17605/OSF.IO/VYBDR (https://osf.io/vybdr/).
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http://dx.doi.org/10.1097/MD.0000000000026340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213302PMC
June 2021

The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy.

Front Immunol 2021 27;12:635326. Epub 2021 May 27.

Clinical Research Institute, The First People's Hospital of Foshan, Foshan, China.

Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In the present study, we recruited 66 MN patients before any treatment and 11 healthy control (HC) and analyzed multiple aspects of the immunoglobulin heavy chain (IGH) repertoire of these samples using high-throughput sequencing. We found that the abnormalities of CDR-H3 length, hydrophobicity, somatic hypermutation (SHM), and germ line index were progressively more prominent in patients with MN, and the frequency of in post-therapy patients was significantly lower than that in pre-therapy patients. Moreover, we found that the gene was significantly related to PLA2R, which is the most commonly used biomarker. The most important discovery was that several , transcripts, CDR-H3 length, and SHM rate in pre-therapy patients had the potential to predict the therapeutic effect. Our study further demonstrated that the IGH repertoire could be a potential biomarker for prognosis prediction of MN. The landscape of circulating B-lymphocyte repertoires sheds new light on the detection and surveillance of MN.
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http://dx.doi.org/10.3389/fimmu.2021.635326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190383PMC
May 2021

Phosphorus doped nickel selenide for full device water splitting.

J Colloid Interface Sci 2021 Jun 10;602:115-122. Epub 2021 Jun 10.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China. Electronic address:

The lack of high active and stable electrocatalysts has impeded the development of electrochemical water splitting device, which is promising technique for renewable energy conversion system. Here, we report a one-step protocol to synthesize P doped NiSe (P-NiSe) by selenylation process derived from nickel foam with assistant of NaHPO and Se powder. The P-NiSe could be directly used as working electrode and shows the superior electrochemical activity, offering current density of 10 mA cm with overpotential of 270 mV for OER and 71 mV for HER. The enhanced electrochemical activity can be ascribed to the P atom doping. The P atom doping leads to the high valence state of Ni active sites, which have high catalytic ability towards OER. Moreover, the P doping makes the d-band center of Ni atoms in P-NiSe move close to Fermi level, facilitating the HER kinetics with respect to proton adsorption and hydrogen desorption. When employed P-NiSe as both anodic and cathodic electrode in alkaline water electrolyzer, a current density of 10 mA cm can be achieved at 1.58 V. Our work highlights the importance of P doping in determining the surface electron configuration for full device water splitting and the facile synthesis protocol would be promising for realistic applications.
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http://dx.doi.org/10.1016/j.jcis.2021.06.013DOI Listing
June 2021

TiO Nanofiber-Modified Lithium Metal Composite Anode for Solid-State Lithium Batteries.

ACS Appl Mater Interfaces 2021 Jun 10;13(24):28398-28404. Epub 2021 Jun 10.

Institute of New Energy for Vehicles, Shanghai Key Laboratory of Development & Application for Metallic Functional Materials, School of Materials Science and Engineering, Tongji University, Shanghai 201804, China.

Solid-state lithium metal batteries (SSLMBs), using lithium metal as the anode and garnet-structured LiLaZrTaO (LLZTO) as the electrolyte, are attractive and promising due to their high energy density and safety. However, the interface contact between the lithium metal and LLZTO is a major obstacle to the performance of SSLMBs. Here, we successfully improve the interface wettability by introducing one-dimensional (1D) TiO nanofibers into the lithium metal to obtain a Li-lithiated TiO composite anode (Li-TiO). When 10 wt % TiO nanofibers are added, the formed composite anode offers a seamless interface contact with LLZTO and enables an interfacial resistance of 27 Ω cm, which is much smaller than 374 Ω cm of pristine lithium metal. Due to the enhanced interface wettability, the symmetric Li-TiO|LLZTO|Li-TiO cell upgrades the critical current density to 2.2 mA cm and endures stable cycling over 550 h. Furthermore, by coupling the Li-TiO composite anode with the LiFePO cathode, the full cell shows stable cycling performance. This work proves the role of TiO nanofibers in enhancing the interface contact between the garnet electrolyte and the lithium metal anode and improving the performance of SSLMBs and provides an effective approach with 1D additives for solving the interface issues.
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http://dx.doi.org/10.1021/acsami.1c07761DOI Listing
June 2021

[Study of the root position of palatally malposed maxillary lateral incisor based on cone-beam CT images].

Shanghai Kou Qiang Yi Xue 2021 Apr;30(2):129-134

Department of Orthodontics, Shanghai Stomatological Hospital; Oral Biomedical Engineering Laboratory, Fudan University. Shanghai 200001, China.

Purpose: The goal of this investigation was to measure and analyze the root position of palatally malposed maxillary lateral incisor based on cone-beam CT(CBCT)images, in order to provide references for orthodontists to move this kind of teeth scientifically.

Methods: CBCT data from 200 patients meeting the selection criteria with palatally malposed maxillary lateral incisor were investigated in this study. The root was divided into eight equal parts by length, then T1 to T8 were orderly pointed from root apex to alveolar ridge crest. The labial and palatal bone thickness at each point was measured; meanwhile, the angle between the long axis of the tooth and that of the alveolar bone was measured.The data was analyzed using SPSS 19.0 software package.

Results: The mean labial bone thicknesses at all researched points were less than 1.00 mm, except for point T1,T7 and T8. The mean labial bone thicknesses at point T3, T4 and T5 were the thinnest, which were all less than 0.5 mm(P<0.05). The mean thicknesses of labial bone gradually increased from T4 to T8(P<0.05). The mean palatal bone thicknesses were all more than 1.00 mm at the eight points,the mean thicknesses of palatal bone gradually increased from T8 to T1(P<0.05). All the angulations between the long axes of teeth and those of the alveolar bone were negative, indicating the root was close to the labial alveolar wall. The average angulation was minus 31.06 degrees.

Conclusions: Results suggest that the root of palatally malposed maxillary lateral incisor is close to the labial wall of the alveolar bone, its labial alveolar bone is frequently quite thin or even deficient, especially in the zone between 1/4 root length to the root apex and the mid-root. If we move the palatally malposed maxillary lateral incisor labially, it is better to choose tipping movement, instead of bodily movement, in order to avoid serious bone fenestration and dehiscence or root absorption.
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April 2021

Author Correction to: Gray-matter structure in long-term abstinent methamphetamine users.

BMC Psychiatry 2021 Jun 9;21(1):299. Epub 2021 Jun 9.

Mental Health Center, West China Hospital of Sichuan University, Chengdu, 610041, China.

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http://dx.doi.org/10.1186/s12888-021-03250-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188652PMC
June 2021

Clinical characteristics and predictive factors of erythema nodosum in granulomatous lobular mastitis.

Australas J Dermatol 2021 Jun 9. Epub 2021 Jun 9.

Department of Mammary Disease, The Second affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Background/objectives: In recent years, there is a growing incidence of granulomatous lobular mastitis (GLM), but studies about the coexistence of erythema nodosum (EN) and GLM are rare. In this study, we assess the clinical characteristics and predictive factors of EN in GLM.

Methods: A total of 303 patients diagnosed with GLM were enrolled from January 2012 to October 2018 at the second affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, including 78 patients with EN. Follow-up data included: lesion site, lesion size, therapy approaches, course of GLM, course of EN, the recurrence of disease, possible causes. All patients had pathologic confirmation of GLM based on core needle biopsy (CNB) or surgical excision.

Result: Fever in the EN group was significantly more common compared to the non-EN group (44.87% vs 12.89%, P < 0.001). The proportion of lesion range >2 quadrants in the EN group was significantly higher than that in the non-EN group (42.31% vs 16.00%, P < 0.001). The course of the disease was longer in the EN group compared to the non-EN group (10.32 moths vs 8.74 moths, P < 0.001). Patients with EN had a trend towards a higher risk of recurrence (5.13% vs 1.33%, P = 0.055).

Conclusion: EN is more likely to be found in GLM patients with breast lesion range >2 quadrants. The presence of EN in GLM indicates that the condition becomes more severe and the course of GLM also becomes longer.
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http://dx.doi.org/10.1111/ajd.13640DOI Listing
June 2021

extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells.

Neural Regen Res 2022 Jan;17(1):203-209

Department of Ophthalmology, Li Ka Shing Faculty of Medicine; State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong Special Administrative Region, China.

Lycium barbarum (LB) is a traditional Chinese medicine that has been demonstrated to exhibit a wide variety of biological functions, such as antioxidation, neuroprotection, and immune modulation. One of the main mechanisms of Alzheimer's disease is that microglia activated by amyloid beta (Aβ) transform from the resting state to an M1 state and release pro-inflammatory cytokines to the surrounding environment. In the present study, immortalized microglial cells were pretreated with L. barbarum extract for 1 hour and then treated with oligomeric Aβ for 23 hours. The results showed that LB extract significantly increased the survival of oligomeric Aβ-induced microglial cells, downregulated the expression of M1 pro-inflammatory markers (inducible nitric oxide synthase, tumor necrosis factor α, interleukin-6, and interleukin-1β), and upregulated the expression of M2 anti-inflammatory markers (arginase-1, chitinase-like protein 3, and interleukin-4). LB extract also inhibited the oligomeric Aβ-induced secretion of tumor necrosis factor α, interleukin-6, and interleukin-1β in microglial cells. The results of in vitro cytological experiments suggest that, in microglial cells, LB extract can inhibit oligomeric Aβ-induced M1 polarization and concomitant inflammatory reactions, and promote M2 polarization.
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http://dx.doi.org/10.4103/1673-5374.314325DOI Listing
January 2022

An initial assessment of the involvement of transglutaminase2 in eosinophilic bronchitis using a disease model developed in C57BL/6 mice.

Sci Rep 2021 Jun 7;11(1):11946. Epub 2021 Jun 7.

Sino-French Hoffmann Institute, Guangzhou, China.

The detailed pathogenesis of eosinophilic bronchitis (EB) remains unclear. Transglutaminase 2 (TG2) has been implicated in many respiratory diseases including asthma. Herein, we aim to assess preliminarily the relationship of TG2 with EB in the context of the development of an appropriate EB model through ovalbumin (OVA) sensitization and challenge in the C57BL/6 mouse strain. Our data lead us to propose a 50 μg dose of OVA challenge as appropriate to establish an EB model in C57BL/6 mice, whereas a challenge with a 400 μg dose of OVA significantly induced asthma. Compared to controls, TG2 is up-regulated in the airway epithelium of EB mice and EB patients. When TG2 activity was inhibited by cystamine treatment, there were no effects on airway responsiveness; in contrast, the lung pathology score and eosinophil counts in bronchoalveolar lavage fluid were significantly increased whereas the cough frequency was significantly decreased. The expression levels of interleukin (IL)-4, IL-13, IL-6, mast cell protease7 and the transient receptor potential (TRP) ankyrin 1 (TRPA1), TRP vanilloid 1 (TRPV1) were significantly decreased. These data open the possibility of an involvement of TG2 in mediating the increased cough frequency in EB through the regulation of TRPA1 and TRPV1 expression. The establishment of an EB model in C57BL/6 mice opens the way for a genetic investigation of the involvement of TG2 and other molecules in this disease using KO mice, which are often generated in the C57BL/6 genetic background.
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http://dx.doi.org/10.1038/s41598-021-90950-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184915PMC
June 2021

Inter-regulated d-band centers of the NiB/Ni heterostructure for boosting hydrogen electrooxidation in alkaline media.

Chem Sci 2020 Oct 8;11(44):12118-12123. Epub 2020 Oct 8.

College of Chemistry and Molecular Sciences, Wuhan University Wuhan Hubei 430072 P. R. China

NiB/Ni heterostructures have been constructed, which exhibit exceptional catalytic performance toward the hydrogen oxidation reaction (HOR) under alkaline media, with the mass activity being about 10 times greater than that of NiB and Ni, respectively, ranking among the most active platinum-group-metal-free electrocatalysts. Experimental results and theoretical calculations confirm electron transfer from NiB to Ni at the NiB/Ni interface, resulting in inter-regulated d-band centers of these two components. This inter-regulation gives rise to optimized binding energies of intermediates, which together contribute to enhanced alkaline HOR activity.
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http://dx.doi.org/10.1039/d0sc03917aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162945PMC
October 2020

Comparison of Granular Bone Grafts and Transverse Process Bone Grafts for Single-Segmental Thoracic Tuberculosis: A Retrospective Single-Center Comparative Study.

Front Surg 2021 14;8:602513. Epub 2021 May 14.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

To compare the clinical efficacy of granular bone grafts and transverse process bone grafts for single-segmental thoracic tuberculosis (TB). The clinical records of 52 patients who were diagnosed with single-segmental thoracic TB and treated by one stage posterior debridement, bone graft fusion, and internal fixation in our department from 2015 to 2018 were retrospectively analyzed. Among them, 25 cases were in the granular bone graft group and 27 cases in the transverse processes bone graft group. Outcomes including the visual analog scale (VAS), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), neurological function, operative time, operative blood loss, hospital stay, Cobb angle, bone graft fusion time, and postoperative complications were all recorded and analyzed. There were no significant differences in operative time, operative blood loss, and hospital stay between the two groups ( > 0.05). With an average follow-up of 18-33 months, all patients in the two groups showed significant improvement in VAS score, ESR, CRP, and neurological function compared with preoperative measurements ( < 0.05), however, no significant differences were found for the last follow-up ( > 0.05). The two groups showed similar Cobb angle correction ( > 0.05), but the granular bone graft group had a larger Cobb angle loss than the transverse processes bone graft group ( < 0.05). The bone graft fusion time of the granular bone graft group was shorter than that of the transverse processes bone graft group ( < 0.05). No significant difference was found in the postoperative complications rate between the two groups ( > 0.05). Granular bone grafts and transverse process bone grafts may achieve comparable clinical efficacy for single-segmental thoracic TB, but the former method had a shorter bone fusion time.
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http://dx.doi.org/10.3389/fsurg.2021.602513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160445PMC
May 2021

Risk Factors for Death Among the First 80 543 COVID-19 Cases in China: Relationships Between Age, Underlying Disease, Case Severity, and Region.

Clin Infect Dis 2021 May 27. Epub 2021 May 27.

Hubei Center for Disease Control and Prevention, 6 North Zuodaoquan, Hongshan District, Wuhan 430079, China.

Background: Knowledge of COVID-19 epidemiology remains incomplete and crucial questions persist. We aimed to examine risk factors for COVID-19 death.

Methods: A total of 80 543 COVID-19 cases reported in China, nationwide, through April 8, 2020 were included. Risk factors for death were investigated by Cox proportional hazards regression and stratified analyses.

Results: Overall national case fatality ratio (CFR) was 5.64%. Risk factors for death were older age (≥80: adjusted hazard ratio [aHR]=12.58, 95% confidence interval [CI]=6.78-23.33), presence of underlying disease (aHR=1.33, CI=1.19-1.49), worse case severity (severe: aHR=3.86, CI=3.15-4.73; critical: aHR=11.34, CI=9.22-13.95), and near-epicenter region (Hubei: aHR=2.64, CI=2.11-3.30; Wuhan: aHR=6.35, CI=5.04-8.00). CFR increased from 0.35% (30-39 years) to 18.21% (≥70 years) without underlying disease. Regardless of age, CFR increased from 2.50% for no underlying disease to 7.72% for 1, 13.99% for 2, and 21.99% for ≥3. CFR increased with worse case severity from 2.80% (mild), to 12.51% (severe) and 48.60% (critical) regardless of region. Compared to other regions, CFR was much higher in Wuhan regardless of case severity (mild: 3.83% versus 0.14% in Hubei and 0.03% elsewhere; moderate: 4.60% versus 0.21% and 0.06%; severe: 15.92% versus 5.84% and 1.86%; and critical: 58.57% versus 49.80% and 18.39%).

Conclusions: Older patients regardless of underlying disease and patients with underlying disease regardless of age were at elevated risk of death. Higher death rates near the outbreak epicenter and during the surge of cases reflect the deleterious effects of allowing health systems to become overwhelmed.
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http://dx.doi.org/10.1093/cid/ciab493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244662PMC
May 2021