Publications by authors named "Wei Li"

14,801 Publications

  • Page 1 of 1

Gut dysbiosis in rheumatic diseases: A systematic review and meta-analysis of 92 observational studies.

EBioMedicine 2022 May 17;80:104055. Epub 2022 May 17.

Department of Orthopaedics, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, China; Hunan Key Laboratory of Joint Degeneration and Injury, Changsha, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China. Electronic address:

Background: Emerging evidence suggests that dysbiosis in gut microbiota may contribute to the occurrence or development of several rheumatic diseases. Since gut microbiota dysbiosis is potentially modifiable, it has been postulated to be a promising preventive or therapeutic target for rheumatic diseases. However, the current understanding on the potential associations between gut microbiota and rheumatic diseases is still inadequate. Therefore, we aimed to synthesise the accumulating evidence for the relation of gut microbiota to rheumatic diseases.

Methods: The PubMed, Embase and Cochrane Library were searched from inception to March 11, 2022 to include observational studies evaluating the associations between gut microbiota and rheumatic diseases. Standardised mean difference (SMD) of α-diversity indices between rheumatic diseases and controls were estimated using random-effects model. β-diversity indices and relative abundance of gut microbes were summarised qualitatively.

Findings: Of the included 92 studies (11,998 participants), 68 provided data for α-diversity. Taken together as a whole, decreases in α-diversity indices were consistently found in rheumatic diseases (observed species: SMD = -0.36, [95%CI = -0.63, -0.09]; Chao1: SMD = -0.57, [95%CI = -0.88, -0.26]; Shannon index: SMD = -0.33, [95%CI = -0.48, -0.17]; Simpson index: SMD = -0.32, [95%CI = -0.49, -0.14]). However, when specific rheumatic diseases were examined, decreases were only observed in rheumatoid arthritis (observed species: SMD = -0.51, [95%CI = -0.78, -0.24]; Shannon index: SMD = -0.31, [95%CI = -0.49, -0.13]; Simpson index: SMD = -0.31, [95%CI = -0.54, -0.08]), systemic lupus erythematosus (Chao1: SMD = -1.60, [95%CI = -2.54, -0.66]; Shannon index: SMD = -0.63, [95%CI = -1.08, -0.18]), gout (Simpson index: SMD = -0.64, [95%CI = -1.07, -0.22]) and fibromyalgia (Simpson index: SMD = -0.28, [95%CI = -0.44, -0.11]), whereas an increase was observed in systemic sclerosis (Shannon index: SMD = 1.25, [95%CI = 0.09, 2.41]). Differences with statistical significance in β-diversity were consistently reported in ankylosing spondylitis and IgG4-related diseases. Although little evidence of disease specificity of gut microbes was found, shared alterations of the depletion of anti-inflammatory butyrate-producing microbe (i.e., Faecalibacterium) and the enrichment of pro-inflammatory microbe (i.e., Streptococcus) were observed in rheumatoid arthritis, Sjögren's syndrome and systemic lupus erythematosus.

Interpretation: Gut microbiota dysbiosis was associated with rheumatic diseases, principally with potentially non-specific, shared alterations of microbes.

Funding: National Natural Science Foundation of China (81930071, 81902265, 82072502 and U21A20352).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ebiom.2022.104055DOI Listing
May 2022

Reference values of low body mass index, mid-upper arm circumference, and calf circumference in cancer patients: A nationwide multicenter observational study.

Nutrition 2022 Apr 11;99-100:111688. Epub 2022 Apr 11.

Department of Gastrointestinal Surgery/Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: Anthropometric measurements including body mass index (BMI), mid-upper arm circumference (MUAC), and calf circumference (CC) are simple and convenient indicators of nutritional status and muscle mass. However, most of their reference values come from studies based on healthy Western populations. The optimal reference values of these anthropometric factors in Asian patients with cancer are unclear. The aim of this study was to develop reference values of severely and moderately low BMI, MUAC, and CC by analyzing a large sample of patients with cancer from a nationwide population.

Methods: We conducted a retrospective analysis of 16 104 patients who were diagnosed with malignant diseases from June 2012 to January 2019. The median age of the patients was 58 y, and 52.5% were men. Optimal stratification was used to calculate reference values using X-tile software. Kaplan-Meier and multivariate Cox analysis were performed to analyze survival data. A receiver operating characteristic analysis was conducted to test the performance of new reference values in diagnosing malnutrition.

Results: The optimal reference values were calculated for BMI (moderately low: 19.7 [women] and 19 [men]; severely low: 16.7 [women] and 16.7 [men]), MUAC (moderately low: 24.5 [women] and 23.2 [men] severely low: 20.6 [women] and 19.4 [mnen]), and CC (moderately low: 29.1 [women] and 29.3 [men]; severely low: 26.7 [women] and 26.9 [men]). New reference values had more significant affects on mortality risk and better performance in predicting malnutrition than existing ones.

Conclusions: The present study defined reference values of moderately and severely low BMI, MUAC, and CC, which showed strong associations with quality of life, malnutrition, and mortality risk. New reference values from the present study are classification references specifically for the Asian population, which is a new step to promote the application of Global Leadership Initiative on Malnutrition criteria and its severity grading system in Asia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nut.2022.111688DOI Listing
April 2022

2D mesoporous materials.

Natl Sci Rev 2022 May 22;9(5):nwab108. Epub 2021 Jun 22.

Department of Chemistry, Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, China.

2D mesoporous materials, combining the unique features of 2D and mesoporous structures, represent a new research direction in materials science. This perspective article highlights the latest progress of 2D mesoporous materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/nsr/nwab108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113154PMC
May 2022

A Systematic Review and Network Meta-Analysis about the Efficacy and Safety of Hook F in Rheumatoid Arthritis.

Evid Based Complement Alternat Med 2022 10;2022:3181427. Epub 2022 May 10.

The Affiliated Hospital of Guizhou Medical University, Guiyang 550001, China.

Objective: This study aims to evaluate the efficacy of various conventional synthetic DMARDs, including Hook F (TwHF) for treating rheumatoid arthritis (RA) by network meta-analysis.

Methods: We retrieved the related literature from online databases and supplemented it by using a manual retrieval method. Data was extracted from the literature and analyzed with STATA software.

Results: A total of 21 trials (5,039 participants) were identified. Assessment of ACR20 response found that TwHF combined with methotrexate (MTX) had the greatest probability for being the best treatment option among the treatments involved, while TwHF used singly was second only to TwHF combined with MTX. Assessment of ACR50 response found that TwHF combined with MTX ranked second in all treatment options after cyclosporine A (CsA) combined with leflunomide (LEF) and TwHF alone, followed by TwHF combined with MTX. Assessment of ACR70 response found that CsA combined with LEF ranked first, TwHF combined with LEF ranked second, TwHF combined with MTX ranked third, and TwHF used singly ranked fourth. In the safety analysis, TwHF had the least probability of adverse event occurrence, followed by TwHF combined with MTX, which ranked first and second, respectively.

Conclusion: Compared with the current csDMARDs for treating RA, the efficacy of TwHF was clear, and TwHF combined with MTX performed well under various endpoints. In the future, large, rigorous, and high-quality RCTs are still needed to confirm the benefits of TwHF therapy on RA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/3181427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113883PMC
May 2022

The Influence of Precipitate Morphology on the Growth of Austenite Grain in Nb-Ti-Al Microalloyed Steels.

Materials (Basel) 2022 Apr 27;15(9). Epub 2022 Apr 27.

Long Products Research Institute, R&D Center, Baoshan Iron & Steel Co., Ltd., Shanghai 201900, China.

The present study investigates the morphological evolution of carbonitrides and the effect of these precipitates on grain boundary pinning during pseudo-carburizing a Nb-Ti-Al microalloyed steel. The result indicated that three kinds of complex precipitates with different morphologies containing Nb, Ti, and Al respectively were observed in samples austenitized at different temperatures and times. The NbC and TiN precipitates played an important role in pinning grain boundaries and suppressing the growth of austenite grains, relying on the high thermal stability of TiN precipitates and small size of NbC precipitates. The precipitate characteristics affected the size of austenite grain. Based on the Zener pinning model, the effect of precipitate characteristic on austenite grain size was quantitatively analyzed. It is found that the existence of NbC and TiN precipitated at high temperature makes austenite grain growth difficult when austenite grain boundaries were pinned by fine and diffused precipitates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma15093176DOI Listing
April 2022

Inversion of Nitrogen Concentration in Apple Canopy Based on UAV Hyperspectral Images.

Sensors (Basel) 2022 May 4;22(9). Epub 2022 May 4.

National Apple Engineering and Technology Research Center, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.

As the major nutrient affecting crop growth, accurate assessing of nitrogen (N) is crucial to precise agricultural management. Although improvements based on ground and satellite data nitrogen in monitoring crops have been made, the application of these technologies is limited by expensive costs, covering small spatial scales and low spatiotemporal resolution. This study strived to explore an effective approach for inversing and mapping the distributions of the canopy nitrogen concentration (CNC) based on Unmanned Aerial Vehicle (UAV) hyperspectral image data in a typical apple orchard area of China. A Cubert UHD185 imaging spectrometer mounted on a UAV was used to obtain the hyperspectral images of the apple canopy. The range of the apple canopy was determined by the threshold method to eliminate the effect of the background spectrum from bare soil and shadow. We analyzed and screened out the spectral parameters sensitive to CNC, including vegetation indices (VIs), random two-band spectral indices, and red-edge parameters. The partial least squares regression (PLSR) and backpropagation neural network (BPNN) were constructed to inverse CNC based on a single spectral parameter or a combination of multiple spectral parameters. The results show that when the thresholds of normalized difference vegetation index (NDVI) and normalized difference canopy shadow index (NDCSI) were set to 0.65 and 0.45, respectively, the canopy's CNC range could be effectively identified and extracted, which was more refined than random forest classifier (RFC); the correlation between random two-band spectral indices and nitrogen concentration was stronger than that of other spectral parameters; and the BPNN model based on the combination of random two-band spectral indices and red-edge parameters was the optimal model for accurately retrieving CNC. Its modeling determination coefficient (R) and root mean square error (RMSE) were 0.77 and 0.16, respectively; and the validation R and residual predictive deviation (RPD) were 0.75 and 1.92. The findings of this study can provide a theoretical basis and technical support for the large-scale, rapid, and non-destructive monitoring of apple nutritional status.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s22093503DOI Listing
May 2022

Analysis of the Effect of Velocity on the Eddy Current Effect of Metal Particles of Different Materials in Inductive Bridges.

Sensors (Basel) 2022 Apr 29;22(9). Epub 2022 Apr 29.

School of Marine Engineering, Dalian Maritime University, Dalian 116026, China.

A method for analyzing the influence of velocity changes on metal signals of different materials in oil detection technology is proposed. The flow rate of metal contaminants in the oil will have a certain impact on the sensitivity of the output particle signal in terms of electromagnetic fields and circuits. The detection velocity is not only related to the sensitivity of the output particle signal, but also to the adaptability of high-speed and high-throughput in oil online monitoring. In this paper, based on a high-sensitivity inductive bridge, the eddy current effect of velocity in a time-harmonic magnetic field is theoretically analyzed and experimentally verified, the phenomenon of particle signal variation with velocity for different materials is analyzed and discussed, and finally the effect of velocity on the output signal of the processing circuit is also elaborated and experimentally verified. Experiments show that under the influence of the time-harmonic magnetic field, the increase of the velocity enhances the detection sensitivity of non-ferromagnetic metal particles and weakens the detection sensitivity of non-ferromagnetic particles. Under the influence of the processing circuit, different velocities will produce different signal gains, which will affect the stability of the signal at different velocities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s22093406DOI Listing
April 2022

Identification and validation of a major QTL for kernel length in bread wheat based on two F biparental populations.

BMC Genomics 2022 May 19;23(1):386. Epub 2022 May 19.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.

Background: High yield and quality are essential goals of wheat (Triticum aestivum L.) breeding. Kernel length (KL), as a main component of kernel size, can indirectly change kernel weight and then affects yield. Identification and utilization of excellent loci in wheat genetic resources is of great significance for cultivating high yield and quality wheat. Genetic identification of loci for KL has been performed mainly through genome-wide association study in natural populations or QTL mapping based on genetic linkage map in high generation populations.

Results: In this study, an F biparental population derived from the cross between an EMS mutant BLS1 selected from an EMS-induced wheat genotype LJ2135 (derived from the hybrid progeny of a spelt wheat (T. spelta L.) and a common wheat) mutant bank and a local breeding line 99E18 was used to rapidly identify loci controlling KL based on Bulked Segregant Analysis (BSA) and the wheat 660 K single-nucleotide polymorphism (SNP) array. The highest ratio of polymorphic SNPs was located on chromosome 4A. Linkage map analysis showed that 33 Kompetitive Allele Specific PCR markers were linked to the QTL for KL (Qkl.sicau-BLE18-4A) identified in three environments as well as the best linear unbiased prediction (BLUP) dataset. This QTL explained 10.87-19.30% of the phenotypic variation. Its effect was successfully confirmed in another F population with the two flanking markers KASP-AX-111536305 and KASP-AX-110174441. Compared with previous studies and given that the of BLS1 has the genetic background of spelt wheat, the major QTL was likely a new one. A few of predicted genes related to regulation of kernel development were identified in the interval of the detected QTL.

Conclusion: A major, novel and stable QTL (Qkl.sicau-BLE18-4A) for KL was identified and verified in two F biparental populations across three environments. Significant relationships among KL, kernel width (KW) and thousand kernel weight (TKW) were identified. Four predicted genes related to kernel growth regulation were detected in the interval of Qkl.sicau-BLE18-4A. Furthermore, this study laid foundation on subsequent fine mapping work and provided a possibility for breeding of elite wheat varieties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-022-08608-3DOI Listing
May 2022

The nutrition-inflammation marker enhances prognostic value to ECOG performance status in overweight or obese patients with cancer.

JPEN J Parenter Enteral Nutr 2022 May 19. Epub 2022 May 19.

Department of Gastrointestinal Surgery/Department of Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

Background: Overweight or obese cancer patients are more likely to develop a proinflammatory status. The aim of this study was to investigate whether nutrition-inflammation marker can provide additional prognostic information on top of well-established ECOG-PS in overweight or obese patients with cancer.

Methods: A total of 1667 overweight or obese cancer patients were enrolled in this study. We assessed the prediction accuracy of ten nutrition-inflammation markers by time-dependent receiver operating characteristic (ROC), and elucidate their association with overall survival (OS) by the Kaplan-Meier method and a Cox model.

Results: In this analysis, the majority of patients had a good performance status (ECOG-PS score ≤1) (88.3%). Both the area under ROC curves and the C-index of the lymphocyte-C-reactive protein ratio (LCR) demonstrated that LCR was the most significant nutrition-inflammation marker correlated with survival. In overweight or obese cancer patients with good ECOG-PS, a low LCR was significantly associated with poorer prognosis (HR; 1.77, 95% CI: 1.47, 2.15, P<0.001), and enhanced the predictive ability of 1-year mortality. For specific tumor types, a low LCR was an independent prognostic factor for lung cancer, upper gastrointestinal cancer and colorectal cancer, and tended to be a significant predictor for breast cancer. In addition, those patients with a combined low LCR and poorer ECOG-PS (ECOG-PS score>1) showed the worst prognosis.

Conclusions: The LCR is more strongly associated with overall survival than other nutrition-inflammation markers, and it is able to further detect patients with worse prognosis on top of ECOG-PS in overweight or obese patients with cancer.

Clinical Relevancy Statement: As overweight or obese patients with cancer are more likely to develop a proinflammatory status, indicators of systemic inflammation might be useful to predict survival outcome for these patients. This study found LCR was the most significant nutrition-inflammation marker correlated with survival in overweight or obese patients with cancer. A low LCR was significantly associated with poorer prognosis, and enhanced the predictive ability of mortality. In addition, the assessment of LCR could contribute to enhanced detection of patients with poorer prognosis on top of ECOG-PS in overweight or obese cancer patients. Since the LCR can be measured easily and inexpensively, it might be a useful prognostic indicator in overweight and obese patients with cancer. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jpen.2407DOI Listing
May 2022

Dopamine-derived nanoparticles for the protection of irradiation-induced intestinal injury by maintaining intestinal homeostasis.

Biomater Sci 2022 May 19. Epub 2022 May 19.

The PLA Rocket Force Characteristic Medical Center, Beijing, China.

Radiotherapy of abdominal and pelvic tumors almost inevitably injures the intestine by oxidative stress and causes inflammation. Regrettably, traditional radioprotective agents for irradiation (IR) induced intestinal injury suffer from challenges such as poor solubility, unsatisfactory bioactivity and undesired adverse reactions, which significantly limit their usefulness. Polydopamine nanoparticles (PDA-NPs) have shown promising potential in scavenging reactive oxygen species (ROS) and suppressing inflammation. In this study, PDA-NPs were prepared by a simple method and their physical properties were characterized. Mice received two doses of PDA-NPs by oral gavage 22 h apart, and were irradiated with X-rays 2 h after the last gavage. The protective effect of PDA-NPs and possible mechanisms of protection against IR-induced intestinal injury were explored. The results showed that PDA-NPs were spherical and well dispersed, with good shape uniformity, compact structure, good colloid dispersion stability, concentration-dependent light absorption, and accurate quantification. Importantly, PDA-NPs reduced mortality and prolonged the average survival time of mice after IR. Furthermore, PDA-NPs protected mice from IR-induced injury to crypt-villus units and maintained intestinal barrier function in the intestine. In particular, PDA-NPs significantly inhibited the depletion of Lgr5 intestinal stem cells (ISCs) and promoted cell regeneration after IR, which indicated that the regeneration ability of ISCs was maintained and the repair of intestinal structure and function was promoted. Finally, PDA-NPs significantly suppressed the apoptosis, inflammatory pyroptosis and DNA damage of intestinal cells induced by ionizing radiation. Altogether, our study suggested that PDA-NPs may have great potential in protecting the intestines from ionizing radiation damage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1bm02026aDOI Listing
May 2022

Ccdc38 is required for sperm flagellum biogenesis and male fertility in mice.

Development 2022 May 19. Epub 2022 May 19.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

The sperm flagellum is essential for male fertility, and defects in flagellum biogenesis are associated with male infertility. Deficiency of CCDC42 is specifically associated with malformation of mouse sperm flagella. Here, we find that the testis-specific expressed protein CCDC38 (coiled-coil domain containing 38) interacts with CCDC42, localizing on the manchette and sperm tail during spermiogenesis. Inactivation of CCDC38 in male mice results in distorted manchette, multiple morphological abnormalities of the flagella (MMAF) of spermatozoa, and eventually male sterility. Furthermore, we find that CCDC38 interacts with intra-flagellar transport protein 88 (IFT88), as well as outer dense fibrous 2 (ODF2), and the knockout of Ccdc38 reduces transport of ODF2 to the flagellum. Altogether, our results uncover the essential role of CCDC38 in sperm flagellum biogenesis, and suggest that some defects of these genes might be associated with male infertility in humans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1242/dev.200516DOI Listing
May 2022

Plasma Drug Concentration of Propranolol and Genetic Study in Chinese Han Patients With Infantile Haemangioma.

Front Pediatr 2022 2;10:849496. Epub 2022 May 2.

Department of Dermatology, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Background And Purpose: This study was conducted to explore the plasma drug concentration of propranolol in Chinese Han patients with infantile haemangioma (IH) and the influencing factors, as well as the relationship among plasma drug concentrations of propranolol, β1-AR mutation and CYP2D6 188C>T, efficacy, and safety.

Experimental Approach: From January 2018 to April 2019, 140 patients with IH who were admitted to the hospital for oral propranolol and agreed to have their plasma concentration of propranolol tested, including 112 patients with β1-AR and CYP2D6 gene tested.

Key Results And Conclusions And Implications: The mean peak blood levels of propranolol, 4-hydroxypropranolol (4-OH-P), and N-deisopropylpropranolol (NDP) were 60.35 ± 37.90, 1.90 ± 2.37, and 0.24 ± 0.18 ng/ml, respectively. The mean trough blood levels of propranolol, 4-OH-P, and NDP were 24.98 ± 17.68, 0.45 ± 0.52, and 0.05±0.05 ng/ml, respectively. The higher the dose of propranolol, the higher the plasma concentration of propranolol ( = 0.031). The plasma concentration of propranolol was not related to the treatment efficacy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2022.849496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108385PMC
May 2022

Breaking scaling relationships in alkynol semi-hydrogenation by manipulating interstitial atoms in Pd with d-electron gain.

Nat Commun 2022 May 18;13(1):2754. Epub 2022 May 18.

The Education Ministry Key Laboratory of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Normal University, Shanghai, 200234, China.

Pd catalysts are widely used in alkynol semi-hydrogenation. However, due to the existence of scaling relationships of adsorption energies between the key adsorbed species, the increase in conversion is frequently accompanied by side reactions, thereby reducing the selectivity to alkenols. We report that the simultaneous increase in alkenol selectivity and alkynol conversion is achieved by manipulating interstitial atoms including B, P, C, S and N in Pd catalysts. A negative linear relationship is observed between the activation entropies of 2-methyl-3-butyn-2-ol and 2-methyl-3-buten-2-ol which is highly related to the filling of d-orbital of Pd catalysts by the modification of p-block elements. A catalyst co-modified by B and C atoms has the maximum d charge of Pd that achieves a 17-fold increase in the turn-over frequency values compared to the Lindlar catalysts in the semi-hydrogenation of 2-methyl-3-butyn-2-ol. When the conversion is close to 100%, the selectivity can be as high as 95%.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-30540-zDOI Listing
May 2022

Mechanical properties, degradation behavior and cytocompatibility of biodegradable 3vol%X (X = MgO, ZnO and CuO)/Zn matrix composites with excellent dispersion property fabricated by graphene oxide-assisted hetero-aggregation.

Mater Sci Eng C Mater Biol Appl 2022 Feb 18:112722. Epub 2022 Feb 18.

National Demonstration Center for Experimental Function Materials Education, Tianjin University of Technology, Tianjin 300384, China. Electronic address:

Metal matrix composites have been recognized as a feasible approach to obtain a new generation of biodegradable Zn-based material. Nevertheless, there is a great challenge in achieving good dispersion properties of the bioactive reinforcements within zinc matrix. A novel and facile approach, namely graphene oxide (GO)-assisted hetero-aggregation, were developed to achieve uniformly dispersed nanoceramics in the Zn matrix, by using very low-content (0.03 vol%) GO as a linker between the Zn matrix and reinforcement. The negatively-charged GO becomes a suitable "bridge" connected the positively-charged metallic powder and bioactive reinforcement by charge neutralization in polarity solvent. Three kinds of reinforcements, including MgO, ZnO and CuO, were used to verify the feasibility of the above-mentioned method. As-sintered 3CuO/Zn matrix composites, which possessed uniformly distributed reinforcement, uniaxial compressive strength of 301.2 MPa, failure strain over 40%, moderate corrosion rate of 0.063 mm·y, acceptable cytocompatibility and antibacterial property, should be a useful material for orthopedic applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msec.2022.112722DOI Listing
February 2022

Multiple Charge Transfer Bands Induced Broad Excitation Eu Red Emission in a Vanadium Phosphate System for White Light-Emitting Diodes.

Inorg Chem 2022 May 18. Epub 2022 May 18.

Collaborative Innovation Center of Rare-Earth Optical Functional Materials and Devices Development, School of Physics and Opto-Electronic Technology, Baoji University of Arts and Sciences, Baoji 721016, PR China.

In order to realize broad excitation and narrow emission red light phosphor, a new vanadium phosphate BaBiVPO was selected as a host for Eu. Monitored at 619 nm, a wide band from 240 to 400 nm could be observed and inferred to be composed of Eu-O and V-O charge transfer bands, which could make it match well with the UV chip and the blue chip along with the characteristic excitation of Eu at 465 nm. Under 354 nm excitation, the sample could emit high color purity red light, and the thermal quenching integral intensity showed good thermal stability. The generation of charge transfer bands was investigated in detail combined with the luminescence properties and the structure of the matrix. Moreover, the as-prepared phosphor could improve the white light performance of blue chip-activated YAG:Ce and -UV chip-activated tricolor phosphors. All the results indicated the multiple application potential of BaBiVPO:Eu for white light-emitting diodes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.2c00730DOI Listing
May 2022

Laparoscopic Management of Spontaneous Colonic Perforation: A Single Institution Study of 52 Patients.

Surg Laparosc Endosc Percutan Tech 2022 Apr 25. Epub 2022 Apr 25.

Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Spontaneous colonic perforation (SCP) is associated with an devastating result. The use of laparoscopy for SCP remains controversial. This study aimed to compare the postoperative outcomes of patients who received either laparoscopic or open surgery and to evaluate the efficacy of laparoscopic surgery and the risk factors affecting prognosis.

Patients And Methods: A retrospective study of patients who underwent surgery for SCP from January 2005 to December 2020 was performed. Demographic data, intraoperative variables, length of stay, and surgical complications were retrieved.

Results: A total of 52 patients were postoperatively diagnosed with SCP. Thirty (57.69%) procedures were performed using laparoscopic surgery (group A) and 22 (42.31%) were performed using open surgery (group B). There were no significant differences between groups A and B in terms of age, sex, chronic concomitant disease, chronic constipation, incentives, imaging findings, preoperative diagnosis, American Society of Anesthesiologists (ASA) score, Mannheim Peritonitis Index (MPI), operation time, days to resumption of liquids, site of perforation, surgical procedures, or types of perforation (P>0.05). The incidence of wound infection in group A was significantly lower than that in group B (P<0.05), but there was no significant difference in the incidence of abdominal abscess between the 2 groups (P>0.05). Significant differences were found in days to start walking and days to resumption of solids between the 2 groups (P<0.05). Group B had a longer length of hospital stay than group A (P<0.05). After multivariate analysis, the independent variables associated with worse perioperative complications were an age of 65 years and older, an ASA score of ≥3, and an MPI of >26.

Conclusions: The prognosis of SCP is poor. The operation should follow principles that are simple, rapid, and effective. If there are no contraindications, laparoscopy may be the preferred method. Hartmann procedure is a promising surgical strategy. The age, ASA score, and MPI may indicate the severity and prognosis of SCP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/SLE.0000000000001057DOI Listing
April 2022

and Metabolic Activation and Hepatotoxicity of Environmental Pollutant 2,6-Dimethylphenol.

Chem Res Toxicol 2022 May 18. Epub 2022 May 18.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P. R. China.

2,6-Dimethylphenol (2,6-DMP) is an environmental pollutant found in industrial wastewater. Exposure to 2,6-DMP is of increasing concern as it endangered reportedly some aquatic animals. In this study, we investigated the metabolic activation and hepatotoxicity of 2,6-DMP. 2,6-DMP was metabolized to an -quinone methide intermediate in vitro and in vivo. The electrophilic metabolite was reactive to the sulfhydryl groups of glutathione, -acetyl cysteine, and cysteine. NADPH was required for the formation of the reactive metabolite. The quinone methide intermediate reacted with cysteine residues to form hepatic protein adduction. A single dose of 2,6-DMP induced marked elevation of serum ALT and AST in mice. Both the protein adduction and hepatotoxicity of 2,6-DMP showed dose dependency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.chemrestox.2c00048DOI Listing
May 2022

Dangling Octahedra Enable Edge States in 2D Lead Halide Perovskites.

Adv Mater 2022 May 18:e2201666. Epub 2022 May 18.

School of Materials Science and Engineering, Tianjin Key Laboratory of Metal and Molecule-Based Material Chemistry, Nankai University, Tianjin, 300350, China.

The structural reconstruction at the crystal layer edges of 2D lead halide perovskites (LHPs) leads to unique edge states (ES) which are manifested by prolonged carrier lifetime and reduced emission energy. These special ES could effectively enhance the optoelectronic performance of devices, but their intrinsic origin and working mechanism remain elusive. Here we demonstrate that the ES of a family of 2D Ruddlesden-Popper LHPs [BA CsPb Br , BA MAPb Br and BA MA Pb Br (BA = butylammonium; MA = methylammonium)] arise from the rotational symmetry elevation of the PbBr octahedra dangling at the crystal layer edges. These dangling octahedra give rise to localized electronic states that enable an effective transport of electrons from the interior to layer edges, and the population of electrons in both the intrinsic states and ES can be manipulated via controlling the external fields. Moreover, the abundant phonons, activated by the dangling octahedra, can interact with electrons to facilitate radiative recombination, counterintuitive to the suppressive role commonly observed in conventional semiconductors. This work unveils the intrinsic atomistic and electronic origins of ES in 2D LHPs, which could stimulate the exploration of ES-based exotic optoelectronic properties and corresponding design of high-performance devices for these emergent low-dimensional semiconductors. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202201666DOI Listing
May 2022

A diverse English keyword search reveals the value of scriptaid treatment for porcine embryo development following somatic cell nuclear transfer.

Reprod Fertil Dev 2022 May 18. Epub 2022 May 18.

Context: Incomplete epigenetic reprogramming of histone deacetylation (HDAC) is one of the main reasons for the low efficiency of somatic cell nuclear transfer (SCNT). Scriptaid is a synthetic HDAC inhibitor (HDACi) that may improve the efficiency of porcine SCNT.

Aims: This study aimed to determine whether scriptaid increases the number of blastocyst cells or the cleavage rate.

Methods: We conducted a meta-analysis of the pertinent literature published over the past decade.

Key Results: A total of 73 relevant papers were retrieved using a diverse English keyword search, and 11 articles were used for the meta-analysis. Scriptaid was positively correlated with blastocyst rate but had no effect on cleavage rate or blastocyst cell number. A subgroup analysis of blastocyst cell number showed that the staining method was the source of the heterogeneity.

Conclusions: In SCNT embryos, scriptaid treatment after activation can promote embryonic development, but there may be adverse effects on early development.

Implications: HDACi research should focus on SCNT birth efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1071/RD22025DOI Listing
May 2022

Brevibacillus dissolubilis sp. nov., Isolated from Fresh Water.

Curr Microbiol 2022 May 17;79(7):194. Epub 2022 May 17.

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.

A Gram-positive-staining, strictly aerobic, motile, ellipsoidal endospore-forming bacterial strain, designated CHY01, was isolated from the Chishui river in a section of Maotai Town, Guizhou Province, Southwest China. Strain CHY01 was found to grow optimally at pH 8.0 and 28 °C. The 16S rRNA gene sequence analysis indicated that strain CHY01 belonged to the genus Brevibacillus and clustered with the type strain of Brevibacillus panacihumi, with which it exhibited 16S rRNA gene sequence similarity values of 97.8%. The predominant respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were C, iso-C, anteiso-C, C, Ciso-H and/or C 3-OH, and C and/or C. Genome sequencing revealed a genome size of 6.1 Mbp and a G + C content of 50.6%. The results of physiological and biochemical tests allowed strain CHY01 to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Pairwise determined whole-genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values suggested that strain CHY01 represents a new species, for which we propose the name Brevibacillus dissolubilis sp. nov. with the type strain CHY01 (= CGMCC 1.15916 = KCTC 33863).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00284-022-02891-9DOI Listing
May 2022

Interleukin-38 promotes skin tumorigenesis in an IL-1Rrp2-dependent manner.

EMBO Rep 2022 May 17:e53791. Epub 2022 May 17.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, China.

Interleukin-38 (IL-38) is strongly associated with chronic inflammatory diseases; however, its role in tumorigenesis is poorly understood. We demonstrated that expression of IL-38, which exhibits high expression in the skin, is downregulated in human cutaneous squamous cell carcinoma and 7,12-dimethylbenzanthracene/12-O-tetradecanoyl phorbol-13-acetate-induced mouse skin tumorigenesis. IL-38 keratinocyte-specific knockout mice displayed suppressed skin tumor formation and malignant progression. Keratinocyte-specific deletion of IL-38 was associated with reduced expression of inflammatory cytokines, leading to reduced myeloid cell infiltration into the local tumor microenvironment. IL-38 is dispensable for epidermal mutagenesis, but IL-38 keratinocyte-specific deletion reduces proliferative gene expression along with epidermal cell proliferation and hyperplasia. Mechanistically, we first demonstrated that IL-38 activates the c-Jun N-terminal kinase (JNK)/activator protein 1 signal transduction pathway to promote the expression of cancer-related inflammatory cytokines and proliferation and migration of tumor cells in an IL-1 receptor-related protein 2 (IL-1Rrp2)-dependent manner. Our findings highlight the role of IL-38 in the regulation of epidermal cell hyperplasia and pro-tumorigenic microenvironment through IL-1Rrp2/JNK and suggest IL-38/IL-1Rrp2 as a preventive and potential therapeutic target in skin cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15252/embr.202153791DOI Listing
May 2022

Occurrence of per- and polyfluoroalkyl substances (PFAS) in municipal solid waste landfill leachates from western China.

Environ Sci Pollut Res Int 2022 May 16. Epub 2022 May 16.

Key Laboratory of Three Gorges Reservoir Region's Eco-Environment Under Ministry of Education, Chongqing University, Chongqing, 400044, China.

Landfill leachate has been documented as a significant source of trace organic pollutants, comprising an expansive family of per- and polyfluoroalkyl substances (PFAS). This study presents the findings on the distribution of 13 perfluoroalkyl carboxylates (PFCAs) and 4 perfluoroalkyl sulfonates (PFSAs) in leachates from 6 municipal solid waste (MSW) landfills in western China. The total concentrations of 17 PFAS in sampled leachates ranged from 1805 to 43,310 ng/L, and 15 compounds were detected in all samples. The short-chain compounds perfluorobutane sulfonate (PFBS, mean mass fraction 23.1%) and perfluorobutyric acid (PFBA, mean mass fraction 20.6%) were dominant. There were higher PFAS concentrations in leachates from operating landfills (mean: 12,194 ng/L) compared to closed landfills (mean: 2747 ng/L), but there was no significant difference between young (< 10 years) and old landfills (> 10 years). Moderate to weak correlations were observed between PFAS concentrations and leachate properties, e.g., TN, NH-N, TOC, and pH. This is the first report on the distribution of PFAS in landfill leachates from western China. The results have identified landfill leachate as an underestimated source of PFAS in the environment and have contributed to a more comprehensive evaluation on PFAS presence across China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-022-20754-5DOI Listing
May 2022

High-Entropy Perovskite as a High-Performing Chromium-Tolerant Cathode for Solid Oxide Fuel Cells.

ACS Appl Mater Interfaces 2022 May 16. Epub 2022 May 16.

Mechanical & Aerospace Engineering Department, West Virginia University, Morgantown, West Virginia 26506, United States.

To achieve chromium tolerance and high performance, a new series of high-entropy perovskites (HEPs) are investigated as cathode materials for solid oxide fuel cells (SOFCs). Multiple rare-earth, alkaline-earth, and high-order transition metal elements are used for the A-site of this ABO structure. A pure phase is achieved through the designed combination of different elements in seven out of eight candidates. Due to the retaining of alkaline-earth elements Sr and/or Ba, the electrical conductivities of these HEPs are in the order of 100 S/cm at 550-700 °C, a value that can practically eliminate the electronic resistance of the porous cathode. Three out of eight candidates show similar or better performance than the (LaSr)(CoFe)O (LSCF) benchmark. It is found that A-site elements can cast a substantial influence on the overall performance even with a change as small as 10% of the total cations. It seems that each element has its individual "phenomenal activity" that can be transferred from one candidate to the other in the general setting of the perovskite structure, leading to the best candidate by using the three most active elements simultaneously at the A-site. Excellent Cr tolerance has been observed on the (LaSrPrYBa)CoFeO sample, showing degradation of only 0.25%/kh during a 41 day operation in the presence of Cr, while LSCF increases by 100% within the first day in the same condition. X-ray photoelectron spectroscopy discovers no Sr segregation as LSCF is found in this HEP; rather, the active element Y takes more A-sites on the outermost layer after long-term operation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c03657DOI Listing
May 2022

Advances in the study of CD47-based bispecific antibody in cancer immunotherapy.

Immunology 2022 May 16. Epub 2022 May 16.

Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Tumor therapy has entered the era of immunotherapy. Monoclonal antibodies (mAb), immune checkpoint inhibitors, chimeric antigen receptor T-cell (CAR-T), cytokine-induced killer (CIK),tumor-infiltrating lymphocytes (TIL) and other cellular immunotherapies have become the focus of current research. The CD47/SIRPα target is becoming another popular tumor immunotherapy target following the PDCD1/CD247(PD1/PD-L1) checkpoint inhibitor. In recent years, a large number of CD47/SIRPα mAbs, fusion proteins, and CD47/SIRPα-based bispecific antibodies (BsAbs) are undergoing preclinical and clinical trials and have good curative effects in the treatment of hematological tumors and solid tumors. They bring new vitality and hope for the treatment of patients with advanced tumors. This review summarizes the research progress of CD47/SIRPα-based BsAbs with different targets for tumor treatment. There are 12 and 9 BsAbs in clinical trials and pre-clinical research, respectively. We report on the mechanism of 15 BsAb molecules with different target and analyze the efficacy and safety of preclinical and clinical trials, discuss the issues that may be faced in the development of CD47-based BsAbs, and dual target molecules, and summarize their development prospects. This review provides a reference for the safety and effectiveness of BsAbs in clinical application and in the future development of antibodies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/imm.13498DOI Listing
May 2022

Noise exposure and its relationship with postinfarction cardiac remodeling: implications for NLRP3 inflammasome activation.

Bioengineered 2022 May;13(5):12127-12140

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

In recent years, high-decibel noise has emerged as a causative risk factor for ischemic heart disease. Massive noise overdose is associated with increased endocrine, neural, and immune stress responses. The NLRP3 (nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain containing 3) inflammasome, the most characterized supramolecular complex and a potent mediator of inflammatory signaling, has been reported to be a marker of increased ischemic heart disease vulnerability. Our study evaluated the association of noise exposure with postinfarction cardiac remodeling and its effect on NLRP3 inflammasome activation. Rats were exposed to a noisy environment (14 days, 24 h/per day, 70 ± 5 dB), and speck formation by the NLRP3 inflammasome scaffold protein ASC (apoptosis-associated speck-like protein) was assessed by confocal immunofluorescence. Echocardiography, pathological analysis, and in vivo electrophysiology were performed. Our results revealed the improved postinfarction cardiac function, mitigated fibrosis, and decreased arrhythmia vulnerability and sympathetic sprouting in low-environment noise groups. Moreover, western blotting of NLRP3, caspase-1, ASC, IL-1β, and IL-18 and confocal microscopy of ASC speck showed that the priming and activation of NLRP3 inflammasome were higher in the NE group than in the NI group. In conclusion, our findings reveal a previously unidentified association between NLRP3 inflammasome activation and noise exposure, underscoring the significance of effective noise prevention in improving postinfarction prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2022.2073126DOI Listing
May 2022

Impaired left ventricular systolic synchrony in patients with lupus Nephritis: A speckle tracking echocardiography study.

Lupus 2022 May 15:9612033221102713. Epub 2022 May 15.

Department of Medical Ultrasonics, The First Affiliated Hospital, 71068Sun Yat-sen University, Guangzhou, China.

Objectives: We aimed to explore the value of two-dimensional speckle tracking echocardiography measurements of the global longitudinal strain (GLS) and left ventricular mechanical dispersion (LVMD) in the assessment of early stage left ventricular systolic dysfunction and heterogeneity of myocardial contraction in patients with lupus nephritis (LN).

Methods: Patients with LN and extra-renal systemic lupus erythematosus (SLE) and healthy participants in the control group underwent echocardiography for the traditional measurement of the left ventricular systolic and diastolic function and speckle tracking measurements of the GLS and LVMD. GLS was defined as the average value of the peak strain during systole of the left ventricular 17 segments, and LVMD was defined as the standard deviation. The demographic characteristics including age, sex, and body mass index (BMI) of all the participants were collected. The clinical and laboratory characteristics of the patients with LN were collected.

Results: We included 41 healthy control, 37 patients with extra-renal SLE, and 73 patients with LN. There were statistically significant differences in the GLS and LVMD between the extra-renal SLE and LN groups (GLS -19.36% vs. -17.61%, < 0.001; LVMD 35.62 ms vs 42.96 ms, <0.001). There was a statistically significant difference in the LVMD between the extral-renal SLE and control groups (35.62ms vs 25.51ms, <0.001), but not in GLS (-19.36% vs -19.52%, > 0.05). Multiple regression analyses were conducted in a subset of patients, and 24-hour proteinuria was independently associated with LVMD (β [SE], 0.793 [0.302], < .05).

Conclusions: Patients with LN have more severe myocardial involvement than patients with extra-renal SLE. The asynchrony in myocardial contraction represented by the LVMD can be recognized earlier than that of the overall contractile functional impairment represented by GLS. In patients with LN, the 24-hour proteinuria was associated with LVMD. This indicates that the heterogeneity in the contractile function may be associated with the severity of renal damage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/09612033221102713DOI Listing
May 2022

Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn .

Front Physiol 2022 29;13:885035. Epub 2022 Apr 29.

Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

Salinity is a crucial factor influencing the growth, development, immunity, and reproduction of aquatic organisms; however, little is known about the molecular mechanism of the response to salinity challenge in larvae of the giant freshwater prawn . Herein, larvae cultured in three treatment groups with salinities of 10, 13, and 16‰ (S10, S13, and S16) were collected, and then transcriptome analysis was conducted by RNA-seq. A total of 6,473, 3,830 and 3,584 differentially expressed genes (DEGs) were identified in the S10 vs. S13 comparison, S10 vs. S16 comparison and S13 vs. S16 comparison, respectively. These genes are involved in osmoregulation, energy metabolism, molting, and the immune response. qPCR analysis was used to detect the expression patterns of 16 DEGs to verify the accuracy of the transcriptome data. Protein-protein interaction (PPI) analysis for DEGs and microsatellite marker screening were also conducted to reveal the molecular mechanism of salinity regulation. Together, our results will provide insight into the molecular genetic basis of adaptation to salinity challenge for larvae of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2022.885035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099292PMC
April 2022

Anlotinib for the Treatment of Multiple Recurrent Lumbar and Sacral Cord Hemangioblastomas: A Case Report.

Front Oncol 2022 27;12:859157. Epub 2022 Apr 27.

Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: Hemangioblastoma (HB) is a rare and highly vascularized tumor that originates from the central nervous system as well as other part of the body. They can appear sporadically or as part of von Hippel-Lindau (VHL) disease, a rare hereditary cancer syndrome. Although surgery can cure the majority of HBs, the disease shows a treatment-refractory challenge upon recurrence. HBs express a high amount of vascular endothelial growth factor (VEGF) which is responsible for angiogenesis and subsequently tumor progression. Anti-angiogenic treatment like bevacizumab has showed effect on HB, so we hypothesized that anlotinib could trigger HB regression its inhibitory effect on VEGF.

Case Presentation: We will share our experience in treating a 62-year-old woman with multiple recurrent lumbar and sacral cord HBs. She was treated with anlotinib (8mg qd d1-14, q3w) for three months and her follow up radiological examination demonstrated marked tumor regression which was evaluated as having partial response pursuant to RECIST 1.1 system. She is currently still receiving treatment of anlotinib orally and the lesions continuously reduced.

Conclusion: We have reported that anlotinib can cause significant radiographic response in a patient with multiple recurrent lumbar and sacral cord HBs for the first time. This might enable a novel therapeutic approach for patients with multiple recurrent HB or those with multiple lesions such as in VHL disease which are difficult to resect surgically.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.859157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092942PMC
April 2022

MIP From Influences the Phagocytosis and Chemotaxis of RAW264.7 Macrophages by Regulating the lncRNA GAS5/miR-21/SOCS6 Axis.

Front Cell Infect Microbiol 2022 29;12:810865. Epub 2022 Apr 29.

Department of Clinical Laboratory, Chongqing Emergency Medical Center, Chongqing University Center Hospital, School of Medicine, Chongqing University, Chongqing, China.

Background: The intracellular pathogen () is a causative agent of pneumonia and does great harm to human health. These bacteria are phagocytosed by alveolar macrophages and survive to replicate within the macrophages. Despite macrophage infectivity potentiator (MIP) protein serving as an essential virulence factor during the invasion process of , the regulatory mechanism of MIP protein in the process of bacterial infection to host cells is not yet completely understood. This research thus aims to explore the interaction between MIP and macrophage phagocytosis.

Methods: Through the experiment of the co-culture of RAW264.7 macrophages with different concentrations of MIP, the chemotactic activity of macrophages was detected and the phagocytosis was determined by a neutral red uptake assay. The expression of long noncoding RNA (lncRNA) GAS5, microRNA-21 (miR-21), and suppressor of cytokine signaling (SOCS)6 was determined by qRT-PCR. Target genes were detected by dual luciferase assay.

Results: MIP could reduce the phagocytosis and improve the chemotaxis of RAW264.7 macrophages. The expression of both lncRNA GAS5 and SOCS6 was increased whereas the expression of miR-21 was decreased when macrophages were treated with MIP. Dual luciferase assay revealed that lncRNA GAS5 could interact with miR-21, and SOCS6 served as the target of miR-21. After GAS5 overexpression, the phagocytosis of RAW264.7 treated with MIP was increased whereas the chemotaxis was decreased. In contrast, the opposite results were found in RAW264.7 following GAS5 interference.

Conclusions: The present results revealed that MIP could influence RAW264.7 macrophages on phagocytic and chemotactic activities through the axis of lncRNA GAS5/miR-21/SOCS6.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2022.810865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105720PMC
May 2022

Both the Complexity of Tight Junctions and Endothelial Transcytosis Are Increased During BBB Postnatal Development in Rats.

Front Neurosci 2022 28;16:850857. Epub 2022 Apr 28.

Department of Neurology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, China.

The blood-brain barrier (BBB) comprises a single layer of endothelial cells and maintains a safe and homeostatic environment for proper neuronal function and synaptic transmission. BBB is not a discrete physical barrier, but a complex, dynamic, and adaptable interface. BBB continues to mature under the influence of the neural environment within a short period of time after birth. However, the basic mechanism of BBB formation and maintenance remains a mystery. Early studies have identified two structural characteristics of microvascular endothelium: special tight junctions (TJs) and a very low transcellular vesicle transport rate. Previous studies believed that BBB damage was mainly due to the destruction of tight junctions, and the role of vesicle transcytosis was neglected, so there was a lack of research on its impact on blood-brain barrier. It is urgent to get a better clarification of the unique structural and functional characteristics of the BBB endothelium to explain the role of BBB injury in neurological diseases. RNA sequencing was used to study the molecular characterization of cerebral cortex vascular endothelium by isolating them from neonatal, adolescent and adult rats. For investigation the maintenance mechanism of the BBB, we focused on the cellular and molecular regulation of barrier formation and the two characteristics of microvascular endothelial cells. Interestingly, we found that during the development of the blood-brain barrier, although the tight junctions gradually mature, endothelial cell transcytosis is gradually enhanced, resulting in an increase in the permeability of the blood-brain barrier. This study suggested that under physiological conditions, low vesicle transport is playing an important role in maintaining the integrity of the blood-brain barrier. This study not only summarized the unique characteristics of microvascular endothelial cells, but also illustrated a clarified mechanism of the development and maintenance of BBB which can provide new therapeutic opportunities for central nervous system drug delivery. Raw data of RNA sequencing were deposited in NCBI Sequence Read Archive database (PRJNA790676).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2022.850857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095945PMC
April 2022
-->