Publications by authors named "Wei Huang"

5,116 Publications

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Bifunctional and Self-Supported NiFeP-Layer-Coated NiP Rods for Electrochemical Water Splitting in Alkaline Solution.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

Department of Chemistry, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.

Designing efficient and robust nonprecious metal-based electrocatalysts for overall water electrolysis, which is mainly limited by the oxygen evolution reaction (OER), for hydrogen production remains a major challenge for the hydrogen economy. In this work, a bimetallic NiFeP catalyst is coated on nickel phosphide rods grown on nickel foam (NiFeP@NiP@NF). This self-supported and interfacially connected electrode structure is favorable for mass transfer and reducing electrical resistance during electrocatalysis. The preparation of NiFeP@NiP@NF is optimized in terms of (i) the coprecipitation time of the NiFe Prussian blue analogue layer that serves as phosphides precursor and (ii) the phosphidation temperature. The optimized sample exhibits excellent OER performance delivering current densities of 10 and 100 mA cm at low overpotentials of 227 and 252 mV in 1.0 M KOH, respectively, and maintaining 10 mA cm for more than 120 h without obvious degradation. Moreover, it can also be operated as a hydrogen evolution electrocatalyst, requiring an overpotential of 105 mV at 10 mA cm in the same medium. Thus, the as-prepared material was tentatively utilized as a bifunctional electrocatalyst in a symmetric electrolyzer, requiring a voltage bias of 1.57 V to afford 10 mA cm in 1.0 M KOH, while exhibiting outstanding stability.
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http://dx.doi.org/10.1021/acsami.1c03089DOI Listing
May 2021

Heterostructures between a tin-based intermetallic compound and a layered semiconductor for gas sensing.

Chem Commun (Camb) 2021 May 10. Epub 2021 May 10.

Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China. and Frontiers Science Center for Flexible Electronics (FSCFE) & Shaanxi Institute of Flexible Electronics (SIFE), Northwestern Polytechnical University (NPU), 127 West Youyi Road, Xi'an 710072, China.

SnS2 nanoplates are used as sacrificial templates to facilitate the in situ growth of intermetallic compound Pt3Sn nanoparticles. The Pt3Sn/SnS2 heterostructures show promise for selective NO2 sensing due to the favored gas adsorption and gas-solid charge transfer on Pt3Sn, combined with the optimized film conductance and formation of ohmic-type Pt3Sn/SnS2 heterointerfaces.
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http://dx.doi.org/10.1039/d1cc00015bDOI Listing
May 2021

Effect of Pelvic Bone Marrow Sparing Intensity Modulated Radiation Therapy on Acute Hematologic Toxicity in Rectal Cancer Patients Undergoing Chemo-Radiotherapy.

Front Oncol 2021 22;11:646211. Epub 2021 Apr 22.

Department of Oncology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: While chemo-radiotherapy improves local control in patients with locally advanced rectal cancer, it can also increase acute hematological toxicity (HT), which leads to poor outcomes. Patients receiving bone marrow radiation have been shown to develop acute HT. However, the safety and efficacy of bone marrow sparing is undetermined. The aim of our study was to explore the feasible dosimetric constraints for pelvic bone marrow (PBM) that can be widely used in rectal cancer patients undergoing chemo-radiotherapy.

Methods: 112 rectal cancer patients were selected and divided into the PBM sparing IMRT group (60 cases) and the non-PBM sparing IMRT group (52 cases). All patients underwent pelvic radiotherapy with concurrent capecitabine-based chemotherapy. The PBM dosimetric constraints in the PBM sparing IMRT group were set to:V ≤ 85%, V ≤ 65% and V ≤ 45%. An independent sample t test was applied for the dose-volume parameters, and Chi-squared analysis was applied for clinical parameters and adverse events.

Results: The radiation dose to PBM (V~V, D, <0.05), PBM sub-regions (V~V, D, <0.05) and both femoral heads (V~V, D, <0.05) decreased significantly in the PBM sparing IMRT group compared with that of the non-PBM sparing IMRT group (<0.05). There was no significant difference in any dose-volume parameters of the bladder and small bowel in either groups, and none in the planning target volume (PTV) dose homogeneity and conformity (>0.05). For acute HT observation, the incidence of grade 3 acute HT (χ = 7.094, =0.008) was significantly reduced in patients treated with PBM sparing IMRT compared with patients treated with non-PBM sparing IMRT. There was no statistical difference in the incidence of vomiting, diarrhea, fatigue, anorexia, nausea, hand-foot syndrome, cystitis, perianal pain and perianal dermatitis in patients of both groups (0.05).

Conclusions: Applying PBM dosimetric constraints (V ≤ 85%, V ≤ 65% and V ≤ 45%) can significantly reduce the radiation dose to PBM. The patients treated with PBM sparing IMRT had a lower incidence of acute HT compared with those treated with non-PBM sparing IMRT. Applying the PBM dosimetric constraints proposed by our study can benefits the patients with rectal cancer undergoing capecitabine-based chemo-radiotherapy.
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http://dx.doi.org/10.3389/fonc.2021.646211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101329PMC
April 2021

The Efficacy and Safety of Pulmonary Vasodilators in Pediatric Pulmonary Hypertension (PH): A Systematic Review and Meta-analysis.

Front Pharmacol 2021 23;12:668902. Epub 2021 Apr 23.

Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

We performed a meta-analysis to evaluate the efficacy and safety of pulmonary vasodilators in pediatric pulmonary hypertension (PH) patients. We searched electronic databases including PubMed, EMBASE, and the Cochrane Library up to May 2020, and conducted a subgroup analysis for pulmonary vasodilators or underlying disease. Fifteen studies with 719 pediatric PH patients were included in the meta-analysis. Adverse events did not differ ( = 0.11, = 15%) between the pulmonary vasodilators group and the control group, neither in the subgroups. In total, compared with the control group treatment, pulmonary vasodilators significantly decreased the mortality ( = 0.002), mean pulmonary artery pressure (mPAP, = 0.02), and mechanical ventilation duration ( = 0.03), also improved the oxygenation index (OI, = 0.01). In the persistent pulmonary hypertension of the newborn (PPHN) subgroup, phosphodiesterase type 5 inhibitors (PDE5i) significantly reduced mortality ( = 0.03), OI ( = 0.007) and mechanical ventilation duration ( = 0.004). Administration of endothelin receptor antagonists (ERAs) improved OI ( = 0.04) and mechanical ventilation duration ( < 0.00001) in PPHN. We also found that in the pediatric pulmonary arterial hypertension (PPAH) subgroup, mPAP was pronouncedly declined with ERAs ( = 0.006). Systolic pulmonary artery pressure (sPAP, < 0.0001) and pulmonary arterial/aortic pressure (PA/AO, < 0.00001) were significantly relieved with PDE5i, partial pressure of arterial oxygen (PaO) was improved with prostacyclin in postoperative PH (POPH) subgroup ( = 0.001). Compared with the control group, pulmonary vasodilators could significantly decrease PA/AO pressure ( < 0.00001) and OI ( < 0.00001) in the short-term (duration <7 days) follow-up subgroup, improve mPAP ( = 0.03) and PaO ( = 0.01) in the mid-term (7-30 days) follow-up subgroup, also decrease mortality, mPAP ( = 0.0001), PA/AO pressure ( = 0.0007), duration of mechanical ventilation ( = 0.004), and ICU stay ( < 0.00001) in the long-term follow subgroup (>30 days). Pulmonary vasodilators decrease the mortality in pediatric PH patients, improve the respiratory and hemodynamic parameters, reduce the mechanical ventilation duration.
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http://dx.doi.org/10.3389/fphar.2021.668902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103162PMC
April 2021

Innate Immunity in Diabetic Wound Healing: Focus on the Mastermind Hidden in Chronic Inflammatory.

Front Pharmacol 2021 21;12:653940. Epub 2021 Apr 21.

Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, China.

A growing body of evidence suggests that the interaction between immune and metabolic responses is essential for maintaining tissue and organ homeostasis. These interacting disorders contribute to the development of chronic diseases associated with immune-aging such as diabetes, obesity, atherosclerosis, and nonalcoholic fatty liver disease. In Diabetic wound (DW), innate immune cells respond to the Pathogen-associated molecular patterns (PAMAs) and/or Damage-associated molecular patterns (DAMPs), changes from resting to an active phenotype, and play an important role in the triggering and maintenance of inflammation. Furthermore, the abnormal activation of innate immune pathways secondary to immune-aging also plays a key role in DW healing. Here, we review studies of innate immune cellular molecular events that identify metabolic disorders in the local microenvironment of DW and provide a historical perspective. At the same time, we describe some of the recent progress, such as TLR receptor-mediated intracellular signaling pathways that lead to the activation of NF-κB and the production of various pro-inflammatory mediators, NLRP3 inflammatory via pyroptosis, induction of IL-1β and IL-18, cGAS-STING responds to mitochondrial injury and endoplasmic reticulum stress, links sensing of metabolic stress to activation of pro-inflammatory cascades. Besides, JAK-STAT is also involved in DW healing by mediating the action of various innate immune effectors. Finally, we discuss the great potential of targeting these innate immune pathways and reprogramming innate immune cell phenotypes in DW therapy.
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http://dx.doi.org/10.3389/fphar.2021.653940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097165PMC
April 2021

Improved delivery system for celastrol-loaded magnetic FeO/α-FeO heterogeneous nanorods: HIF-1α-related apoptotic effects on SMMC-7721 cell.

Mater Sci Eng C Mater Biol Appl 2021 Jun 17;125:112103. Epub 2021 Apr 17.

Affiliated Kunshan Hospital, Jiangsu University, Suzhou 215300, PR China. Electronic address:

FeO/α-FeO heterogeneous nanorods were prepared by a rapid combustion method with α-FeOOH nanorods as precursors. FeO/α-FeO heterogeneous nanorods with a saturation magnetization of 33.2 emu·g were obtained using 30 mL of absolute ethanol at a calcination temperature of 300 °C. Their average length was around 140 nm, and average diameter was about 20 nm. To improve the dispersion characteristics of the FeO/α-FeO heterogeneous nanorods in aqueous solution, citric acid and PEG were applied to modify the nanorod surface via the Mitsunobu reaction. The results showed that the hydrodynamic size range of FeO/α-FeO/CA-PEG-celastrol was 250-500 nm, the surface potential was -15 mV, and the saturation magnetization was approximately 23 emu·g. The drug loading capacity of FeO/α-FeO/CA-PEG was larger than the non-PEG modified version. FeO/α-FeO/CA-PEG-celastrol had slow-release characteristics and was sensitive to changes in pH. Application of a magnetic field significantly promoted the inhibition of SMMC-7721 human liver cancer cell growth after treatment with FeO/α-FeO/CA-PEG-celastrol. Celastrol and FeO/α-FeO/CA-PEG-celastrol increased the production of reactive oxygen species in SMMC-7721 cells and promoted apoptosis and apoptosis-related proteins (p53, Bax, Bcl-2) were also changed. In addition, the expression of hypoxia-inducible factor 1α (HIF-1α) was enhanced. We may conclude that celastrol-loaded magnetic FeO/α-FeO heterogeneous nanorods may be applied in the chemotherapy of human cancer with good biocompatibility and delivery.
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http://dx.doi.org/10.1016/j.msec.2021.112103DOI Listing
June 2021

Patterns of MHC-dependent sexual selection in a free-living population of sheep.

Mol Ecol 2021 May 7. Epub 2021 May 7.

Institute of Evolutionary Biology, University of Edinburgh.

The MHC is one of the most polymorphic gene clusters in vertebrates and play an essential role in adaptive immunity. Apart from pathogen-mediated selection, sexual selection can also contribute to the maintenance of MHC diversity. MHC-dependent sexual selection could occur via several mechanisms but at present there is no consensus as to which of these mechanisms are involved and their importance. Previous studies have often suffered from limited genetic and behavioural data and small sample size, and were rarely able to examine all the mechanisms together, determine whether signatures of MHC-based non-random mating are independent of genomic effects or differentiate whether MHC-dependent sexual selection takes place at the pre- or post-copulatory stage. In this study, we use Monte Carlo simulation to investigate evidence for non-random MHC-dependent mating patterns by all three mechanisms in a free-living population of Soay sheep. Using 1710 sheep diplotyped at the MHC class IIa region and genome-wide SNPs, together with field observations of consorts, we found sexual selection against a particular haplotype in males at the pre-copulatory stage and sexual selection against female MHC heterozygosity during the rut. We also found MHC-dependent disassortative mating at the post-copulatory stage, along with strong evidence of inbreeding avoidance at both stages. However, results from generalized linear mixed models suggest that the pattern of MHC-dependent disassortative mating could be a by-product of inbreeding avoidance. Our results therefore suggest that while multiple apparent mechanisms of non-random mating with respect to the MHC may occur, some of them have alternative explanations.
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http://dx.doi.org/10.1111/mec.15938DOI Listing
May 2021

A natural symbiotic bacterium drives mosquito refractoriness to Plasmodium infection via secretion of an antimalarial lipase.

Nat Microbiol 2021 May 6. Epub 2021 May 6.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.

The stalling global progress in the fight against malaria prompts the urgent need to develop new intervention strategies. Whilst engineered symbiotic bacteria have been shown to confer mosquito resistance to parasite infection, a major challenge for field implementation is to address regulatory concerns. Here, we report the identification of a Plasmodium-blocking symbiotic bacterium, Serratia ureilytica Su_YN1, isolated from the midgut of wild Anopheles sinensis in China that inhibits malaria parasites via secretion of an antimalarial lipase. Analysis of Plasmodium vivax epidemic data indicates that local malaria cases in Tengchong (Yunnan province, China) are significantly lower than imported cases and importantly, that the local vector A. sinensis is more resistant to infection by P. vivax than A. sinensis from other regions. Analysis of the gut symbiotic bacteria of mosquitoes from Yunnan province led to the identification of S. ureilytica Su_YN1. This bacterium renders mosquitoes resistant to infection by the human parasite Plasmodium falciparum or the rodent parasite Plasmodium berghei via secretion of a lipase that selectively kills parasites at various stages. Importantly, Su_YN1 rapidly disseminates through mosquito populations by vertical and horizontal transmission, providing a potential tool for blocking malaria transmission in the field.
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http://dx.doi.org/10.1038/s41564-021-00899-8DOI Listing
May 2021

Photosynthetic regulation under fluctuating light at chilling temperature in evergreen and deciduous tree species.

J Photochem Photobiol B 2021 Apr 29;219:112203. Epub 2021 Apr 29.

Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.

Plants usually experience fluctuating light conditions at chilling temperatures during the autumn season. We hypothesized that photosystem I (PSI) and PSII are more susceptible to photoinhibition under fluctuating light at chilling temperatures in deciduous species relative to evergreen species. We measured the photosynthetic performances under fluctuating light at 6 °C in two evergreen and two deciduous broadleaf tree species. Within the first 10 s after light increased at 6 °C, none of these species could generate an enough trans-thylakoid proton gradient. Meanwhile, PSI was highly oxidised in evergreen species but was highly reduced in deciduous species. This transient over-reduction of PSI in deciduous species was mainly caused by the higher electron flow from PSII. Furthermore, the deciduous species showed a significantly smaller violaxanthin pool and lower non-photochemical quenching under high light conditions at 6 °C, leading to more excess light energy could not be dissipated in PSII. Hence, we propose that fluctuating light combined with chilling temperature cause the over-reduction of photosynthetic electron chain in deciduous species.
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http://dx.doi.org/10.1016/j.jphotobiol.2021.112203DOI Listing
April 2021

Bilateral coracohumeral distance discrepancy is associated with subscapularis tear in rotator cuff rupture patients.

Knee Surg Sports Traumatol Arthrosc 2021 May 6. Epub 2021 May 6.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuanjiagang, Yuzhong District, Chongqing, 400016, China.

Purpose: To describe the bilateral coracohumeral morphological discrepancy in rotator cuff rupture patients with and without subscapularis (SSC) involvement and to investigate its association with SSC tears.

Methods: Two hundred and thirteen consecutive patients who were scheduled to have arthroscopic rotator cuff repair were prospectively enrolled in the current study. Patients with acute traumatic rotator cuff rupture, glenohumeral osteoarthritis, bilateral rotator cuff rupture, recurrent shoulder instability, systemic inflammatory disease, and previous shoulder surgery history were excluded. Coracohumeral distance (CHD), coracoid overlap (CO), lesser tuberosity index (LTI) and acromiohumeral interval (AHI) were measured bilaterally using CT scans. Based on arthroscopic findings, patients were included in either the SSC tear group (n = 72) or the control group (n = 141).

Results: In the SSC tear group, the affected shoulder possessed a significantly smaller CHD [95% confidence interval (CI) 6.1-7.2 vs. 7.2-8.0 mm, p < 0.0001], larger LTI (95% CI 9.4-9.9 vs. 9.0-9.6 mm, p < 0.0001), and smaller AHI (95% CI 5.0-5.5 vs. 7.1-7.5 mm, p < 0.0001) than the contralateral normal shoulder. In the control group, there was no significant difference between bilateral CHD and CO, and the AHI bilateral discrepancy was less distinct. CO did not differ significantly in the bilateral comparison in either group. Among all evaluated parameters, bilateral CHD discrepancy was the best predictor of SSC tears, with an area under the curve (AUC) of 0.882. A cutoff value of 0.5 mm had a sensitivity of 76.4% and specificity of 99.3% for SSC tears.

Conclusion: The CHD values are significantly different between affected and contralateral shoulders in SSC tear patients. Bilateral CHD discrepancy is closely associated with subcoracoid impingement and SSC tears, and its presence warrants specific intraoperative SSC inspection.

Level Of Evidence: Level II.
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http://dx.doi.org/10.1007/s00167-021-06597-6DOI Listing
May 2021

Snail/PRMT5/NuRD complex contributes to DNA hypermethylation in cervical cancer by TET1 inhibition.

Cell Death Differ 2021 May 5. Epub 2021 May 5.

Tianjin Key Laboratory of Inflammatory Biology, The province and ministry co-sponsored collaborative innovation center for medical epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.

The biological function of PRMT5 remains poorly understood in cervical cancer metastasis. Here, we report that PRMT5 physically associates with the transcription factor Snail and the NuRD(MTA1) complex to form a transcriptional-repressive complex that catalyzes the symmetrical histone dimethylation and deacetylation. This study shows that the Snail/PRMT5/NuRD(MTA1) complex targets genes, such as TET1 and E-cadherin, which are critical for epithelial-mesenchymal transition (EMT). This complex also affects the conversion of 5mC to 5hmC. This study demonstrates that the Snail/PRMT5/NuRD(MTA1) complex promotes the invasion and metastasis of cervical cancer in vitro and in vivo. This study also shows that PRMT5 expression is upregulated in cervical cancer and various human cancers, and the PRMT5 inhibitor EPZ015666 suppresses EMT and the invasion potential of cervical cancer cells by disinhibiting the expression of TET1 and increasing 5hmC, suggesting that PRMT5 is a potential target for cancer therapy.
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http://dx.doi.org/10.1038/s41418-021-00786-zDOI Listing
May 2021

The Predictive Value of Computed Tomography Enterography Index for Postoperative Intra-Abdominal Septic Complications in Patients with Crohn's Disease: Implications for Surgical Decision Making.

Dis Colon Rectum 2021 Apr 26. Epub 2021 Apr 26.

Department of General Surgery, Jinling Medical School of Nanjing Medical University, No. 305 East Zhongshan Road, Nanjing, PR China Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 East Zhongshan Road, Nanjing, PR China Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, No. 305 East Zhongshan Road, Nanjing, PR China Department of General Surgery, Jinling Hospital, Medical School of Southeast University, No. 305 East Zhongshan Road, Nanjing, PR China.

Background: Postoperative intra-abdominal septic complications in patients with Crohn's Disease undergoing intestinal resection and anastomosis are frequent and difficult to manage.

Objective: This study sought to explore the value of preoperative computed tomography enterography to predict intra-abdominal septic complications.

Design: This was a retrospective and prospective observational study.

Settings: This study was conducted in a tertiary referral hospital.

Patients: Patients with Crohn's Disease undergoing primary intestinal resection were enrolled in our study.

Main Outcome Measures: The computed tomography enterography severity index was calculated and its ability to predict intra-abdominal septic complications evaluated by multivariate analyses. A prospective study was then performed to assess the reliability of this computed tomography enterography index.

Results: The incidence of postoperative intra-abdominal septic complications in patients undergoing a one-stage procedure was significantly higher than those undergoing a two-stage procedure (3/103 vs 24/241; 2.9% vs 10.0%; p=0.026). A multivariate analysis identified five computed tomography enterography parameters including mesenteric fibrofatty proliferation, intra-abdominal abscess or phlegmon, intestinal fistula, peritoneal effusion, and intestinal dilatation with stricture to be independent predictors of intra-abdominal septic complications (p<0.001). A Nomogram model based on these five parameters was constructed. A receiver operating characteristic analysis identified a computed tomography enterography nomogram score cutoff of 175 as a predictor of intra-abdominal septic complications with a sensitivity of 83.3%, a specificity of 85.3%. In the prospective study, those patients with computed tomography enterography nomogram score greater than 175 were assigned to the two-stage group, which resulted in a similar intra-abdominal septic complications incidence in those undergoing intestinal resection with or without anastomosis (2/82 vs 2/34; P= 0.355).

Limitations: This study was limited by its single-center scope.

Conclusion: Preoperative computed tomography enterography findings may predict postoperative outcomes and help determine surgical approach in Crohn's Disease. Patients with worse intra-abdominal findings confirmed by computed tomography enterography may benefit from stoma creation after intestinal resection. See Video Abstract at http://links.lww.com/DCR/B588 .
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http://dx.doi.org/10.1097/DCR.0000000000001796DOI Listing
April 2021

Clinical value of atomization therapy in children with bronchopneumonia.

Minerva Pediatr (Torino) 2021 May 5. Epub 2021 May 5.

Medical Clinic, Air Force Health Care Center for Special Services Hangzhou, Hangzhou, China -

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http://dx.doi.org/10.23736/S2724-5276.21.06373-4DOI Listing
May 2021

Rational design of high performance nanotheranostics for NIR-II fluorescence/magnetic resonance imaging guided enhanced phototherapy.

Biomater Sci 2021 May;9(9):3499-3506

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.

Nanotheranostics, which can provide great insight into cancer therapy, has been deemed as a promising technology to settle the unmet medical needs. The rational design of high performance nanotheranostics with multiple complementary imaging features and satisfactory therapeutic efficacy is particularly valuable. Herein, versatile nanotheranostic agents DPPB-Gd-I NPs were fabricated by using gadolinium-diethylenetriaminepentaacetic acid chelates and an iodine-decorated copolymer as encapsulation matrixes to encapsulate a polymer DPPB through one-step nanoprecipitation. We have demonstrated that such nanoagents are able to efficiently damage tumors under single dose injection and NIR laser illumination conditions due to the enhanced photodynamic therapy and enhanced photothermal therapy (the tumor inhibition rate was as high as 94.5%). Moreover, these nanoagents can be utilized as dual-modal NIR-II fluorescence/magnetic resonance imaging probes for tumor diagnosis with high sensitivity, deep tissue penetration, and excellent spatial resolution. Overall, this work offers a powerful tactic to fabricate high performance nanotheranostics for clinical application.
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http://dx.doi.org/10.1039/d1bm00172hDOI Listing
May 2021

Three coordination polymers built by quaternary-ammonium-modified isophthalic acid.

Acta Crystallogr C Struct Chem 2021 May 9;77(Pt 5):221-226. Epub 2021 Apr 9.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, Jiangsu Province 211800, People's Republic of China.

Three coordination polymers based on quaternary-ammonium-modified isophthalic acid, namely, catena-poly[[[aqua-μ-bromido-di-μ-hydroxido-methanoldinitratotetracopper(II)]-bis{μ-5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylato}] nitrate], {[CuBr(CHNO)(NO)(OH)(CHO)(HO)]NO}, 1, poly[μ-bromido-μ-bromido-bromido-μ-hydroxido-{μ-5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylato}tricopper(II)], [CuBr(CHNO)(OH)], 2, and poly[bromido{μ-5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylato}zinc(II)], [ZnBr(CHNO)], 3, were obtained by solvothermal reactions. Coordination polymer (CP) 1 contains tetranuclear Cu units, in which the four Cu atoms are linked by two μ-OH groups into a Cu(OH) cluster, which are in turn linked by 5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylate (cpa) ligands into a chain structure. CP 2 also contains a tetranuclear Cu(OH) cluster and these are linked with CuBr units into chains. The chains are then connected by cpa ligands into a two-dimensional layered structure. CP 3 contains a two-dimensional layer structure built by binuclear Zn units and cpa ligands. The Br counter-anions of the quaternary ammonium groups all take part in the construction of the polymeric networks.
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http://dx.doi.org/10.1107/S2053229621003296DOI Listing
May 2021

Serum Levels of ITGBL1 as an Early Diagnostic Biomarker for Hepatocellular Carcinoma with Hepatitis B Virus Infection.

J Hepatocell Carcinoma 2021 27;8:285-300. Epub 2021 Apr 27.

Department of Infectious Diseases, Research Laboratory of Clinical Virology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, 200025, People's Republic of China.

Purpose: Early diagnostic biomarkers of hepatocellular carcinoma (HCC) are needed to distinguish hepatitis B virus (HBV) associated HCC (HBV-HCC) patients from at-risk patients. We assessed the diagnostic values of serum Integrin beta-like 1 (ITGBL1) for early-stage HBV-HCC.

Patients And Methods: We recruited 716 participators including 299 in the training and 417 in the validation stage, (HBV-HCC, chronic hepatitis B (CHB), HBV-related liver cirrhosis (HBV-LC), and healthy controls) between 2017 and 2020 from three centers. Serum ITGBL1 was measured by ELISA. Receiver operating characteristic (ROC) was used to calculate diagnostic accuracy.

Results: The serum levels of ITGBL1 in HBV-HCC patients were significantly lower than those in CHB and HBV-LC patients. This result was confirmed in the follow-up patients who progressed from HBV-LC to HCC. The optimum diagnostic cutoff value of serum ITGBL1 was 47.93ng/mL for detection of early-stage HBV-HCC. The serum ITGBL1 has higher diagnostic accuracy than AFP20 in differentiating the early-stage HBV-HCC from the at-risk patients (area under curve [AUC] 0.787 vs 0.638, p<0.05). For AFP-negative (<20ng/mL) HBV-HCC patients, serum ITGBL1 maintained diagnostic accuracy (training cohort: AUC 0.756, 95% confidence interval [CI] 0.683-0.819, sensitivity 68.18%, and specificity 68.85%; validation cohort: 0.744, 0.686-0.796, 81.13%, and 55.88%). Combination ITGBL1 with AFP20 significantly increased diagnostic accuracy in differentiating the HBV-HCC from at-risk patients (AUC 0.840; 0.868) than ITGBL1 (AUC 0.773, p<0.05; 0.732, p<0.0001) or AFP20 (AUC 0.705, p<0.0001; 0.773, p<0.0001) alone.

Conclusion: The serum level of ITGBL1 improved identification of AFP-negative HBV-HCC patients, and increased diagnostic accuracy with AFP20 together in the early detection of HBV-HCC.
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http://dx.doi.org/10.2147/JHC.S306966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088298PMC
April 2021

Parameterized atmospheric oxidation capacity and speciated OH reactivity over a suburban site in the North China Plain: A comparative study between summer and winter.

Sci Total Environ 2021 Jun 23;773:145264. Epub 2021 Jan 23.

Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China; University of the Chinese Academy of Sciences, Beijing 100049, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address:

The atmospheric oxidation capacity (AOC) and photochemical reactivity are of increasing concern owing to their roles in photochemical pollution. The AOC and OH reactivity were evaluated based on simultaneous measurements of volatile organic compounds (VOCs), trace gases and photolysis frequency during summer and winter campaigns at a suburban site in Xianghe. The AOC exhibited well-defined seasonal and diurnal patterns, with higher intensities during the summertime and daytime than during the wintertime and nighttime, respectively. The major reductants contributing to the AOC during the summertime were CO (41%) and alkenes (41%), whereas CO (40%) and oxygenated VOCs (OVOCs) (30%) dominated the AOC during the wintertime. The dominant oxidant contributor to the AOC during the daytime was OH (≥93%), while the contributions of O and NO (≥75%) to the AOC increased during the nighttime. High values during the wintertime and an increase at night were features of the speciated OH reactivity. Inorganic compounds (NO and CO) dominated the speciated OH reactivity (76% and 85% during the summer and winter campaigns, respectively). Among VOCs, the dominant contributors were alkenes (12%) and OVOCs (7%) during the summer and winter campaigns, respectively. The ratio of NO- and VOC-attributed OH reactivity indicated that O formation occurred under a VOC-limited regime during the summertime and that aromatics had the largest potential to form O Isoprene and m/p-xylene were the most important contributors to the AOC, OH reactivity and O-forming among VOCs during the summertime, biogenic sources and secondary formation and industrial production were the main sources of these species. During the wintertime, hexanal and ethylene were the key VOC species contributing to the AOC and OH reactivity, and solvent usage and traffic-related emissions were the main contributing sources. We recommend that priority measures for the control of VOC species and sources should be taken when suitable. CAPSULE: This study focused on the similarities and differences in the AOC and speciated OH reactivity during summer and winter campaigns.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145264DOI Listing
June 2021

Subarachnoid Hemorrhage and Coronavirus Disease 2019: An Analysis of 282,718 Patients.

World Neurosurg 2021 Apr 30. Epub 2021 Apr 30.

Institute for Data Science and Informatics, University of Missouri, Columbia, MO, USA; Department of Medicine, University of Missouri, Columbia, MO, USA; Department of Electrical Eng. and Computer Science, University of Missouri, Columbia, MO, USA.

Background: Intracranial hemorrhage (including subarachnoid hemorrhage) has been reported in 0.3 to 1.2% of patients with coronavirus disease 2019 (COVID-19). However, no study has evaluated the risk of subarachnoid hemorrhage in COVID-19 patients.

Methods: We analyzed the data from 62 healthcare facilities using the Cerner de-identified COVID-19 dataset.

Results: A total of 86 (0.1%) and 376 (0.2%) subarachnoid hemorrhage patients among 85,645 patients with COVID-19 and 197,073 patients without COVID-19, respectively. In the multivariate model, there was a lower risk of subarachnoid hemorrhage in COVID-19 patients (odds ratio [OR] 0.5, 95% confidence interval [CI] 0.4-0.7, p<.0001) after adjusting for gender, age strata, race, hypertension and nicotine dependence/tobacco use. The proportions of patients who developed pneumonia (58.1% versus 21.3%, p<.0001), acute kidney injury (43% versus 27.7%, p=0.0005), septic shock (44.2% versus 20.7%, p<.0001) and respiratory failure (64.0% versus 39.1%, p<.0001) were significantly higher among subarachnoid hemorrhage patients with COVID-19 compared with those without COVID-19. The in-hospital mortality among subarachnoid hemorrhage patients with COVID-19 was significantly higher compared with those without COVID-19 (31.4% versus 12.2%, p<.0001).

Conclusion: The risk of subarachnoid hemorrhage was not increased in patients with COVID-19. The higher mortality in subarachnoid hemorrhage patients with COVID-19 compared with those without COVID-19 is likely mediated by higher frequency of systemic co-morbidities.
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http://dx.doi.org/10.1016/j.wneu.2021.04.089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086384PMC
April 2021

Efficacy and safety of Chinese herbal footbaths for the treatment of dysmenorrhea: Protocol for a systematic review and meta-analysis.

PLoS One 2021 3;16(5):e0250685. Epub 2021 May 3.

Centre for Health, Activity, and Rehabilitation Research, School of Physiotherapy, University of Otago, Dunedin, Otago, New Zealand.

Background: Chinese herbal footbaths are an external therapy of traditional Chinese medicine that has been widely used to treat dysmenorrhea. This review aims to systematically evaluate its efficacy and safety for the treatment of dysmenorrhea.

Methods: Databases of PubMed, EMBASE, Cochrane Library, CIHAHL, Web of Science, Chinese National Knowledge Infrastructure(CNKI), Chinese Scientific Journals Database (VIP), Wanfang Database, China Biomedical Literature Database(CBM), and Chinese Biomedical Literature Service System (SinoMed) will be searched from the inception to September 30, 2020. The eligible randomized controlled trials (RCTs) will be identified and included. The primary outcomes include pain intensity measured by validated scales of visual analog scale, numeric rating scale, and response rate of symptom reduction. The secondary outcomes are scores on validated pain questionnaires, quality of life measured by SF-36 or other validated scales, and adverse events. Study selection, data extraction, and assessment of bias risk will be conducted by two reviewers independently. RevMan software (V.5.3.5) will be utilized to perform data synthesis. Subgroup and sensitivity analysis will be performed when necessary. The strength of the evidence will be evaluated with the Grading of Recommendations Assessment, Development and Evaluation System.

Results: A high-quality synthesis of current evidence of Chinese herbal footbaths for patients with dysmenorrhea will be provided in this study.

Conclusion: This systematic review will provide evidence of whether Chinese herbal footbaths are an effective and safe intervention for the treatment of dysmenorrhea.

Systematic Review Registration: PROSPERO CRD42020188256.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250685PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092769PMC
May 2021

Stress Hyperglycemia Is Independently Associated with Persistent Organ Failure in Acute Pancreatitis.

Dig Dis Sci 2021 May 3. Epub 2021 May 3.

Department and Laboratory of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre and West China-Liverpool Biomedical Research Centre, West China Hospital, Sichuan University, No. 37 Wannan Guoxue Alley, Chengdu, 610041, Sichuan Province, China.

Background/aims: Stress hyperglycemia is common in critical illness but it has not been clearly studied in patients with acute pancreatitis (AP). This study aimed to investigate the specific blood glucose (BG) level that defines stress hyperglycemia and to determine the impact of stress hyperglycemia on clinical outcomes in AP patients.

Methods: AP patients admitted ≤ 48 h after abdominal pain onset were retrospectively analyzed. Patients were stratified by pre-existing diabetes and stress hyperglycemia was defined using stratified BG levels for non-diabetes and diabetes with clinical outcomes compared.

Results: There were 967 non-diabetic and 114 diabetic (10.5%) patients met the inclusion criteria and the clinical outcomes between these two groups were not significantly different. In non-diabetes, the cut-off BG level of ≥ 180 mg/dl was selected to define stress hyperglycemia with an 8.8-fold higher odds ratio for persistent organ failure (POF) (95% CI 5.4-14.3; P < 0.001). For diabetes, ≥ 300 mg/dl was selected with a 7.5-fold higher odds ratio for POF (95% CI 1.7-34.3; P = 0.009). In multivariable logistic regression, stress hyperglycemia was independently associated with POF, acute necrotic collection, major infection and mortality. The combination of BG and systemic inflammatory response syndrome (SIRS) score in predicting POF was better than SIRS or Glasgow score alone.

Conclusions: This study identifies a cut-off BG level of ≥ 180 mg/dl and ≥ 300 mg/dl was optimal to define stress hyperglycemia for non-diabetic and diabetic AP patients, respectively. There was a significant relationship between stress hyperglycemia and adverse clinical outcomes.
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http://dx.doi.org/10.1007/s10620-021-06982-8DOI Listing
May 2021

Pattern of Recurrence in 428 Patients With Thoracic Esophageal Squamous Cell Carcinoma After Radical Surgery and Its Implication in Postoperative Radiotherapeutic Clinical Target Volume.

Front Oncol 2021 15;11:652365. Epub 2021 Apr 15.

Department VI of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Purpose: This study aimed to analyze the recurrence patterns of thoracic esophageal squamous cell carcinoma (ESCC) after radical surgery, and to understand its implication in the clinical target volume (CTV) design of postoperative radiotherapy (PORT) in patients with ESCC.

Methods And Materials: A total of 428 recurrent ESCC patients after radical surgery between 2014 and 2018 were included in this study. Recurrence patterns, especially anastomotic and regional lymph node recurrence (LNR), were analyzed. A T-shaped CTV were proposed for PORT and were evaluated whether it could cover most of regional LNR.

Results: These patients all experienced anastomotic and/or regional LNR. Among the 428 patients, 27 cases (6.3%) had anastomotic recurrence only, and184 cases (43.0%) had LNR only. Those sites with an LNR rate higher than 15% in upper thoracic ESCC were as follows: No.101, No.104R, No.104L, No.106recR, No.106recL, No.106pre, No.106tb, No.107, and No. 109. Those with middle thoracic ESCC were as follows: No.104R, No.104L, 106recR, No.106recL, No.106pre, No.106tb, and No.107. Lastly, individuals with lower thoracic ESCC were as follows: No.104L, 106recR, No.106recL, No. 106pre, No. 106tb, No.107, and abdominal No. 3. The proportion of LNR not included in the proposed T-shaped CTV was 12.5% (1/8), 4.7% (6/128), and 10.4% (5/48) in the upper, middle, and lower thoracic segments, respectively.

Conclusions: LNR was the most common type of local-regional recurrence in patients after radical surgery. Supraclavicular, superior and middle mediastinal lymph nodes had the highest recurrence rate, the rate of LNR which was outside T-shaped PORT CTV we proposed was less than 15%.
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http://dx.doi.org/10.3389/fonc.2021.652365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083983PMC
April 2021

Quantitating the art and science of esthetic clinical success.

J World Fed Orthod 2021 Apr 28. Epub 2021 Apr 28.

Developmental Biology, Harvard School of Dental Medicine, Boston, MA. Electronic address:

Background: Beginning with the biobehavioral bases of esthetic experiences, this article presents a quantitative analytic review of the motives and methods of providers and consumers of orthodontic treatment.

Method: A primary focus is determining the anthropometric bases of self and others' perceived preference and satisfaction with changes in facial appearance. These quantitative analyses have been based on determining the frequency and magnitude of reliability and validity measures of diagnosis, treatment, and satisfaction outcome. Socioeconomic considerations are also quantitated regarding the discrepancy between objective need for treatment as determined for example by the Index of Orthodontic Treatment Need and the subjective demand for treatment.

Results: The major contribution of this article is the quantitation of the components of esthetic experience from sensation of perception using psycho physical methods, such as Perceptometrics, for determining the morphological basis of perceived facial attractiveness adjusted for ethnocultural differences updated by 3-dimensional and artificial intelligence technology. Recent quantitation of smile components has also added to the measures of esthetically successful treatment. Further contribution of orthodontists to mental and physical health is demonstrated by the differences between perceived personality attributes in profile and full-frontal views of symmetric and asymmetric faces. Such information can facilitate the clinician's ability to determine the ideational representation of the patients' perceived pre- and post-treatment outcome.

Conclusion: The quantitative analysis of the motives and methods involved in the orthodontic treatment process has been combined with the neurophysiological correlates of producing and observing/evaluation of the esthetic experiences of both patients and orthodontists/dentists.
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http://dx.doi.org/10.1016/j.ejwf.2021.03.004DOI Listing
April 2021

Protective Effects of Necrostatin-1 in Acute Pancreatitis: Partial Involvement of Receptor Interacting Protein Kinase 1.

Cells 2021 Apr 27;10(5). Epub 2021 Apr 27.

Department of Molecular Physiology & Cell Signalling, Institute of Systems, Molecular & Integrative Biology, University of Liverpool, Liverpool L69 3BX, UK.

Acute pancreatitis (AP) is a severe and potentially fatal disease caused predominantly by alcohol excess and gallstones, which lacks a specific therapy. The role of Receptor-Interacting Protein Kinase 1 (RIPK1), a key component of programmed necrosis (Necroptosis), is unclear in AP. We assessed the effects of RIPK1 inhibitor Necrostatin-1 (Nec-1) and RIPK1 modification (RIPK1: kinase dead) in bile acid (TLCS-AP), alcoholic (FAEE-AP) and caerulein hyperstimulation (CER-AP) mouse models. Involvement of collateral Nec-1 target indoleamine 2,3-dioxygenase (IDO) was probed with the inhibitor Epacadostat (EPA). Effects of Nec-1 and RIPK1 were also compared on pancreatic acinar cell (PAC) fate in vitro and underlying mechanisms explored. Nec-1 markedly ameliorated histological and biochemical changes in all models. However, these were only partially reduced or unchanged in RIPK1 mice. Inhibition of IDO with EPA was protective in TLCS-AP. Both Nec-1 and RIPK1 modification inhibited TLCS- and FAEE-induced PAC necrosis in vitro. Nec-1 did not affect TLCS-induced Ca entry in PACs, however, it inhibited an associated ROS elevation. The results demonstrate protective actions of Nec-1 in multiple models. However, RIPK1-dependent necroptosis only partially contributed to beneficial effects, and actions on targets such as IDO are likely to be important.
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http://dx.doi.org/10.3390/cells10051035DOI Listing
April 2021

On-the-Fly Fusion of Remotely-Sensed Big Data Using an Elastic Computing Paradigm with a Containerized Spark Engine on Kubernetes.

Sensors (Basel) 2021 Apr 23;21(9). Epub 2021 Apr 23.

School of Civil and Hydraulic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Remotely-sensed satellite image fusion is indispensable for the generation of long-term gap-free Earth observation data. While cloud computing (CC) provides the big picture for RS big data (RSBD), the fundamental question of the efficient fusion of RSBD on CC platforms has not yet been settled. To this end, we propose a lightweight cloud-native framework for the elastic processing of RSBD in this study. With the scaling mechanisms provided by both the Infrastructure as a Service (IaaS) and Platform as a Services (PaaS) of CC, the Spark-on-Kubernetes operator model running in the framework can enhance the efficiency of Spark-based algorithms without considering bottlenecks such as task latency caused by an unbalanced workload, and can ease the burden to tune the performance parameters for their parallel algorithms. Internally, we propose a task scheduling mechanism (TSM) to dynamically change the Spark executor pods' affinities to the computing hosts. The TSM learns the workload of a computing host. Learning from the ratio between the number of completed and failed tasks on a computing host, the TSM dispatches Spark executor pods to newer and less-overwhelmed computing hosts. In order to illustrate the advantage, we implement a parallel enhanced spatial and temporal adaptive reflectance fusion model (PESTARFM) to enable the efficient fusion of big RS images with a Spark aggregation function. We construct an OpenStack cloud computing environment to test the usability of the framework. According to the experiments, TSM can improve the performance of the PESTARFM using only PaaS scaling to about 11.7%. When using both the IaaS and PaaS scaling, the maximum performance gain with the TSM can be even greater than 13.6%. The fusion of such big Sentinel and PlanetScope images requires less than 4 min in the experimental environment.
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http://dx.doi.org/10.3390/s21092971DOI Listing
April 2021

Unveiling Cancer Metabolism through Spontaneous and Coherent Raman Spectroscopy and Stable Isotope Probing.

Cancers (Basel) 2021 Apr 5;13(7). Epub 2021 Apr 5.

Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK.

Metabolic reprogramming is a common hallmark in cancer. The high complexity and heterogeneity in cancer render it challenging for scientists to study cancer metabolism. Despite the recent advances in single-cell metabolomics based on mass spectrometry, the analysis of metabolites is still a destructive process, thus limiting in vivo investigations. Being label-free and nonperturbative, Raman spectroscopy offers intrinsic information for elucidating active biochemical processes at subcellular level. This review summarizes recent applications of Raman-based techniques, including spontaneous Raman spectroscopy and imaging, coherent Raman imaging, and Raman-stable isotope probing, in contribution to the molecular understanding of the complex biological processes in the disease. In addition, this review discusses possible future directions of Raman-based technologies in cancer research.
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http://dx.doi.org/10.3390/cancers13071718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038603PMC
April 2021

Highly efficient electrochemiluminescence resonance energy transfer material constructed from an AIEgen-based 2D ultrathin metal-organic layer for thrombin detection.

Chem Commun (Camb) 2021 Apr;57(35):4323-4326

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

A facile strategy to design a highly efficient electrochemiluminescence resonance energy transfer (ECL-RET) system was proposed by using an AIEgen-based 2D ultrathin metal-organic layer (MOL) to coordinatively immobilize energy donors and acceptors simultaneously, in which the distance between adjacent donor-acceptor pairs was precise and short for obtaining high ECL-RET efficiency.
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http://dx.doi.org/10.1039/d1cc00364jDOI Listing
April 2021

Prognostic Modeling of Patients Undergoing Surgery Alone for Esophageal Squamous Cell Carcinoma: A Histopathological and Computed Tomography Based Quantitative Analysis.

Front Oncol 2021 12;11:565755. Epub 2021 Apr 12.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University, Guangzhou, China.

Objective: To evaluate the effectiveness of a novel computerized quantitative analysis based on histopathological and computed tomography (CT) images for predicting the postoperative prognosis of esophageal squamous cell carcinoma (ESCC) patients.

Methods: We retrospectively reviewed the medical records of 153 ESCC patients who underwent esophagectomy alone and quantitatively analyzed digital histological specimens and diagnostic CT images. We cut pathological images (6000 × 6000) into 50 × 50 patches; each patient had 14,400 patches. Cluster analysis was used to process these patches. We used the pathological clusters to all patches ratio (PCPR) of each case for pathological features and we obtained 20 PCPR quantitative features. Totally, 125 computerized quantitative (20 PCPR and 105 CT) features were extracted. We used a recursive feature elimination approach to select features. A Cox hazard model with L1 penalization was used for prognostic indexing. We compared the following prognostic models: Model A: clinical features; Model B: quantitative CT and clinical features; Model C: quantitative histopathological and clinical features; and Model D: combined information of clinical, CT, and histopathology. Indices of concordance (C-index) and leave-one-out cross-validation (LOOCV) were used to assess prognostic model accuracy.

Results: Five PCPR and eight CT features were treated as significant indicators in ESCC prognosis. C-indices adjusted for LOOCV were comparable among four models, 0.596 (Model A) vs. 0.658 (Model B) vs. 0.651 (Model C), and improved to 0.711with Model D combining information of clinical, CT, and histopathology (all p<0.05). Using Model D, we stratified patients into low- and high-risk groups. The 3-year overall survival rates of low- and high-risk patients were 38.0% and 25.0%, respectively (p<0.001).

Conclusion: Quantitative prognostic modeling using a combination of clinical data, histopathological, and CT images can stratify ESCC patients with surgery alone into high-risk and low-risk groups.
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http://dx.doi.org/10.3389/fonc.2021.565755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072145PMC
April 2021

Total and Semisyntheses of Polymyxin Analogues with 2-Thr or 10-Thr Modifications to Decipher the Structure-Activity Relationship and Improve the Antibacterial Activity.

J Med Chem 2021 Apr 28. Epub 2021 Apr 28.

CAS Key Laboratory of Receptor Research, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Pudong, Shanghai 201203, China.

Herein, we report the total and semisyntheses of a series of polymyxin analogues with 2-Thr and 10-Thr modifications to reveal the structure-activity relationship (SAR), which has not been fully elucidated previously. We employed two total-synthetic strategies to facilitate the diversified replacements on 2-Thr or 10-Thr, respectively. Moreover, semisynthetic approaches were utilized to achieve selective esterification of 2-Thr or dual esterification of both 2- and 10-Thr. Based on the results of antibacterial assays, SAR analysis implicated that the replacement of 2-/10-Thr with amino acids carrying hydrophobic side chains can maintain the activity against but had varied effects on other tested Gram-negative bacteria. The aminoacetyl esterification on 2-/10-Thr achieved excellent antibacterial activity, and the compound exhibited 2-8-fold higher activity against different strains and lower toxicity toward the HK-2 cell line. This work explored the SAR of polymyxin 2-/10-Thr and provided a promising strategy for the development of novel polymyxin derivatives.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02217DOI Listing
April 2021

Enzyme-Initiated Assembly of an Extracellular-Like Two-Dimensional Nanonetwork as a Method to Detect Procancerous Activity.

ACS Sens 2021 Apr 28. Epub 2021 Apr 28.

Department of Laboratory Medicine, the Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing 210003, China.

Extracellular matrix (ECM) enzymes such as lysyl oxidase (LOX) provide a new possibility to contain the invasive progress of cancer. Unlike conventional enzymes, the activity of ECM enzymes is not simply the conversion of the substrate to the product; the amount of enzymes such as matrix metalloproteinases in the ECM changes the structural integrity and morphology of the ECM. These are all important aspects that must be monitored in a spatiotemporally coupled fashion to fully understand their procancerous effect. To achieve this goal, a new molecular probe is developed, which, unlike antibodies or aptamers, can interact with the target enzyme in a more interactive way: the probe can withdraw the metal ion cofactor of the enzyme and modulate its catalytic ability. This can lead to self-propagated cross-linking of the probes to form a network not dissimilar to the collagen and elastin network of the ECM, formed through LOX activity. Thus, the biosensing process itself is a biomimetic of what may occur in vivo in the ECM, and three distinct types of signal readouts can be simultaneously recorded on the sensing surface to provide a fuller picture of ECM enzyme activity, not achievable with traditional designs. Using this method, a parallel between the detected signal and the progress of colorectal cancer can be observed. These results may point to prospective application of this method in evaluating ECM-related tumor invasiveness in the future.
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http://dx.doi.org/10.1021/acssensors.0c02651DOI Listing
April 2021

Effects of Shuanghuanglian oral liquids on patients with COVID-19: a randomized, open-label, parallel-controlled, multicenter clinical trial.

Front Med 2021 Apr 28. Epub 2021 Apr 28.

Division of Cardiology, Department of Internal Medicine and Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

We conducted a randomized, open-label, parallel-controlled, multicenter trial on the use of Shuanghuanglian (SHL), a traditional Chinese patent medicine, in treating cases of COVID-19. A total of 176 patients received SHL by three doses (56 in low dose, 61 in middle dose, and 59 in high dose) in addition to standard care. The control group was composed of 59 patients who received standard therapy alone. Treatment with SHL was not associated with a difference from standard care in the time to disease recovery. Patients with 14-day SHL treatment had significantly higher rate in negative conversion of SARS-CoV-2 in nucleic acid swab tests than the patients from the control group (93.4% vs. 73.9%, P = 0.006). Analysis of chest computed tomography images showed that treatment with high-dose SHL significantly promoted absorption of inflammatory focus of pneumonia, which was evaluated by density reduction of inflammatory focus from baseline, at day 7 (mean difference (95% CI), -46.39 (-86.83 to -5.94) HU; P = 0.025) and day 14 (mean difference (95% CI), -74.21 (-133.35 to -15.08) HU; P = 0.014). No serious adverse events occurred in the SHL groups. This study illustrated that SHL in combination with standard care was safe and partially effective for the treatment of COVID-19.
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http://dx.doi.org/10.1007/s11684-021-0853-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079840PMC
April 2021