Publications by authors named "Wei Huang"

5,877 Publications

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Ultrathin two-dimensional hybrid perovskites toward flexible electronics and optoelectronics.

Natl Sci Rev 2022 May 19;9(5):nwab129. Epub 2021 Jul 19.

Key Laboratory of Flexible Electronics and Institute of Advanced Materials, School of Physical and Mathematical Sciences, Nanjing Tech University, China.

Ultrathin hybrid perovskites combine the advantages of 2D morphology and organic-inorganic components. This perspective article provides an updated summary and new insights for their development in flexible electronics and optoelectronics.
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http://dx.doi.org/10.1093/nsr/nwab129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113132PMC
May 2022

Exchange between Interlayer and Intralayer Exciton in WSe/WS Heterostructure by Interlayer Coupling Engineering.

Nano Lett 2022 May 19. Epub 2022 May 19.

Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, China.

Because of type-II band alignment, interlayer exciton (IX) is found in a van der Waals (vdW) heterostructure (HS) formed by two monolayers of transition-metal dichalcogenides. Manipulation of IXs is of great importance for excitonic integrated devices. Here, we demonstrate that high pressure and tensile strain can be applied to enhance and reduce interlayer coupling of WSe/WS HS, respectively. High pressure induces the transform of intralayer excitons to IX, while tensile strain leads to the transform of IXs to intralayer excitons. In addition, there is a direct-to-indirect band gap transition of WSe/WS HS. The interlayer distance of WSe/WS HS is reduced under high pressure, but it increased under uniaxial tensile strain from first-principles calculations. The calculated band structures explain well the transformation between interlayer and intralayer excitons of WSe/WS HS. This work demonstrates the exchange of interlayer and intralayer excitons and paves the way to manipulate excitons of HS for excitonic applications.
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http://dx.doi.org/10.1021/acs.nanolett.2c01353DOI Listing
May 2022

Relationship between perceived stress and depression in Chinese front-line medical staff during COVID-19: A conditional process model.

J Affect Disord 2022 May 15;311:40-46. Epub 2022 May 15.

Shenzhen Mental Health Center, Shenzhen Kangning Hospital, Shenzhen, China. Electronic address:

Background: Sustained stress during COVID-19 may be associated with depression in front-line medical staff, which would expose them to severe threats. This study aimed to examine whether the relationship between perceived stress and depression is mediated by insomnia, and whether this mediation is moderated by resilience.

Methods: For front-line medical staff, this study used online questionnaire to evaluate their perceived stress, depression, insomnia and resilience. A conditional process model was performed to examine the relationship between perceived stress and depression, as well as the mediating effect of insomnia and the moderating effect of resilience.

Results: A total of 606 front-line medical staff completed the survey. Higher level of perceived stress was significantly positively related to severe insomnia and depression. In addition, insomnia was positively related to depression, while resilience could moderate the effect of perceived stress on depression by direct and indirect paths.

Limitations: The causality among perceived stress, depression, insomnia and resilience is difficult to be verified.

Conclusions: Perceived stress is positively related to depression, and insomnia can mediate the effect of perceived stress on depression. In addition, the effect of perceived stress on depression, whether direct or indirect, is moderated by resilience, which is a protective factor for mental health.
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http://dx.doi.org/10.1016/j.jad.2022.05.064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107392PMC
May 2022

Semi-Supervised Neuron Segmentation via Reinforced Consistency Learning.

IEEE Trans Med Imaging 2022 May 18;PP. Epub 2022 May 18.

Emerging deep learning-based methods have enabled great progress in automatic neuron segmentation from Electron Microscopy (EM) volumes. However, the success of existing methods is heavily reliant upon a large number of annotations that are often expensive and time-consuming to collect due to dense distributions and complex structures of neurons. If the required quantity of manual annotations for learning cannot be reached, these methods turn out to be fragile. To address this issue, in this article, we propose a two-stage, semi-supervised learning method for neuron segmentation to fully extract useful information from unlabeled data. First, we devise a proxy task to enable network pre-training by reconstructing original volumes from their perturbed counterparts. This pre-training strategy implicitly extracts meaningful information on neuron structures from unlabeled data to facilitate the next stage of learning. Second, we regularize the supervised learning process with the pixel-level prediction consistencies between unlabeled samples and their perturbed counterparts. This improves the generalizability of the learned model to adapt diverse data distributions in EM volumes, especially when the number of labels is limited. Extensive experiments on representative EM datasets demonstrate the superior performance of our reinforced consistency learning compared to supervised learning, i.e., up to 400% gain on the VOI metric with only a few available labels. This is on par with a model trained on ten times the amount of labeled data in a supervised manner. Code is available at https://github.com/weih527/SSNS-Net.
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http://dx.doi.org/10.1109/TMI.2022.3176050DOI Listing
May 2022

Genome-wide methylation profiling of early colorectal cancer using an Illumina Infinium Methylation EPIC BeadChip.

World J Gastrointest Oncol 2022 Apr;14(4):935-946

Medical Innovation Research Division, Chinese PLA General Hospital, Beijing 100853, China.

Background: DNA methylation is a part of epigenetic modification, that is closely related to the growth and development of colorectal cancer (CRC). Specific methylated genes and methylated diagnostic models of tumors have become current research focuses. The methylation status of circulating DNA in plasma might serve as a potential biomarker for CRC.

Aim: To investigate genome-wide methylation pattern in early CRC using the Illumina Infinium Human Methylation 850K BeadChip.

Methods: The 850K Methylation BeadChip was used to analyze the genome-wide methylation status of early CRC patients ( = 5) and colorectal adenoma patients ( = 5). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analyses were performed on the selected differentially methylated sites to further discover candidate methylation biomarkers in plasma.

Results: A total of 1865 methylated CpG sites with significant differences were detected, including 676 hypermethylated sites and 1189 hypomethylated sites. The distribution of these sites covered from the 1 to 22 chromosomes and are mainly distributed on the gene body and gene promoter region. GO and KEGG enrichment analysis showed that the functions of these genes were related to biological regulation, molecular binding, transcription factor activity and signal transduction pathway.

Conclusion: The study demonstrated that the Illumina Infinium Human Methylation 850K BeadChip can be used to investigate genome-wide methylation status of plasma DNA in early CRC and colorectal adenoma patients.
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http://dx.doi.org/10.4251/wjgo.v14.i4.935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048525PMC
April 2022

Cascading Retro-Diels-Alder Cycloreversion and Sydnone-Maleimide Based Double 1,3-Dipolar Cycloaddition for Quantitative Thermal Cross-Linking of an Amorphous Polymer Solid.

ACS Macro Lett 2013 Mar 5;2(3):256-259. Epub 2013 Mar 5.

Department of Materials Science and Engineering, University of Washington, Seattle, Washington, 98195-2120, United States.

A new approach to polymer cross-linking is investigated using a cascading cycloreversion of a maleimide-furan adduct and a double 1,3-dipolar cycloaddition between a sydnone and maleimide. The cross-linking proceeds quantitatively above 63 °C, despite the polymer possessing no observable glass transition temperature. The resulting polymer network possesses a high thermal stability (>300 °C) due to the irreversibility of the sydnone-maleimide cycloaddition, which releases CO during the cross-linking, driving the reaction. The rigid three-dimensional structure of the bis-maleimide-sydnone cycloadduct produced local free volumes in films, decreasing the dielectric constant of the material.
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http://dx.doi.org/10.1021/mz4000267DOI Listing
March 2013

Advancements on Small Molecule PROTACs Containing an Indole/Spiro-fused Oxoindole Scaffolds: An Emerging Degrader Targeting Cancers.

Anticancer Agents Med Chem 2022 May 9. Epub 2022 May 9.

State Key Laboratory of Southwestern Chinese Medicine Resources, Hospital of Chengdu University of Traditional Chinese Medicine, School of Pharmacy and College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Indole and spiro-fused oxoindole frameworks widely exist in a variety of natural bioactive products, pharmaceuticals and drug candidates, featuring unique functions in the regulation of proliferation, infiltration, and metastasis of cancer cells. In recent years, significant progress of proteolysis targeting chimeric (PROTAC) technology that employs ubiquitin-proteasome system (UPS) to eliminate disease-associated proteins has been witnessed, thus opening a promising avenue to the discovery of new indole-related drugs. In this review, we focus on summarizing the achievements on small molecule PROTACs that involve indole/spiro-fused oxoindole scaffolds in the part of ligands of the protein of interest (POI). Current challenges and future directions in this promising field are discussed at the end of this review. For the convenience of readers, our review is divided into five parts according to the types of target proteins. We hope this review could bring a quick look and some inspiration to researchers in relevant fields.
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http://dx.doi.org/10.2174/1871520622666220509175305DOI Listing
May 2022

Regulatory mechanism of miR-20a-5p expression in Cancer.

Cell Death Discov 2022 May 16;8(1):262. Epub 2022 May 16.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.

MicroRNAs(miRNAs) are non-coding single-stranded RNA molecules encoded by endogenous genes with a length of about 22 nucleotides. The dysregulation of miRNAs has been proven to be one of the vital causes of cancer, which makes them a biomarker for cancer diagnosis and prognosis. Compared with surgery and chemotherapy, nucleic acid therapy targeting specific miRNAs is a promising candidate for cancer treatment. miR-20a-5p plays an anticancer role in high-incidence human cancers such as cervical cancer, breast cancer and leukemia, which is of great importance in the diagnosis of cancers. The up-regulation and down-regulation of miR-20a-5p offers a possible breakthrough for the treatment of cancers. In this paper, we aim to investigate the functional significance of miR-20a-5p in different cancers, reviewing the expression differences of miR-20a-5p in cancer, while systematically summarizing the changes of circRNA-miR-20a-5p networks, and probe how it promotes messenger RNA (mRNA) degradation or inhibits mRNA translation to regulate downstream gene expression. We've also summarized the biogenesis mechanism of miRNAs, and emphasized its role in cell proliferation, cell apoptosis and cell migration. On this basis, we believe that miR-20a-5p is a promising and effective marker for cancer diagnosis, prognosis and treatment.
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http://dx.doi.org/10.1038/s41420-022-01005-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110721PMC
May 2022

HDL and Sepsis.

Adv Exp Med Biol 2022 ;1377:129-139

Department of Cardiology, Chinese PLA Central War Command General Hospital, Wuhan, China.

Sepsis has been recognized as a global health burden in the year 2017 for its high morbidity and mortality. HDL, a cholesterol transporter, also plays vital role in inflammation besides its typical role in reverse cholesterol transportation. In septic patients, HDL levels dropped significantly. HDL exerts a variety of protective effects in the pathophysiology of sepsis including acting on pathogens, inhibiting macrophage inflammatory reaction, and modulating endothelial function in sepsis. Studies have shown that rHDL or apoA-I mimetic peptides could be therapeutic potentials in sepsis. HDL has caused increasing concern as a potential therapeutic agent.
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http://dx.doi.org/10.1007/978-981-19-1592-5_10DOI Listing
May 2022

Low Prevalence of in Dogs From Central China.

Front Cell Infect Microbiol 2022 28;12:885348. Epub 2022 Apr 28.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

Background: can infect almost all warm-blooded animals, including humans and dogs. Humans can become infected with by petting dogs that have eaten or contacted infected cat feces. The aim of this study was to evaluate infections in dogs from central China. From 2015 to 2021, a total of 536 dog samples (195 fecal, 81 hearts, and 260 serum samples) from Henan Province were collected. Heart juice or serum samples (n = 341) were tested for antibodies using the modified agglutination test (MAT). Fresh myocardium (n = 6) and blood (n = 2) samples were bioassayed in mice.

Results: The present study showed that 4.40% (15/341) of the dogs were seropositive for by MAT (cut-off, 1:25) and 4.10% (8/195) of dog feces contained DNA. No DNA was found in any myocardium (n = 81) or blood (n = 2) samples. The viable strain was not isolated from any myocardium or blood samples (n = 8). Compared to the prevalence of antibodies in dogs sampled from 2015 to 2018, the prevalence significantly declined from 2020 to 2021 ( < 0.05). Gender and age were not risk factors for dogs infected with in this study. However, compared to other sources, dogs from Zhoukou City (close to the Yellow River) or from pet shops showed significantly higher prevalence for ( < 0.05).

Conclusion: A total of 4.29% dogs were infected by (23/536, 8 of 195 fecal samples, 2 of 260 serum, and 13 of 81 heart juice samples). This is the first survey of infection in dog feces from China. Dogs were exposed to , and they could act as mechanical transmitters of .
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http://dx.doi.org/10.3389/fcimb.2022.885348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097580PMC
May 2022

Perceived Stress Positively Relates to Insomnia Symptoms: The Moderation of Resilience in Chinese Pregnant Women During COVID-19.

Front Psychiatry 2022 27;13:856627. Epub 2022 Apr 27.

Shenzhen Mental Health Center/Shenzhen Kangning Hospital, Shenzhen, China.

Background: The government's COVID-19 pandemic response lockdown strategy had a negative psychological and physical impact on individuals, which necessitated special care to pregnant women's mental health. There has been no large-scale research on the underlying relationship between perceived stress and insomnia symptoms in pregnant Chinese women up to this point. During the COVID-19 pandemic, we wanted to see if there was an association between perceived stress and insomnia symptoms, as well as the moderating impact of resilience for Chinese pregnant women.

Methods: This cross-sectional study examined 2115 pregnant women from central and western China using multi-stage sampling methodologies. A systematic questionnaire was used to collect information on sleep quality, perceived stress, and resilience using the Insomnia Severity Index, Perceptual Stress Scale, and Connor and Davidson Resilience Scale. To assess the moderating influence of resilience, hierarchical regressions were used.

Results: During the COVID-19 pandemic, 18.53% of respondents ( = 2115) reported experiencing sleeplessness. In pregnant women, perceived stress was positively linked with insomnia symptoms ( < 0.001). Furthermore, resilience significantly attenuated the influence of perceived stress on insomnia symptoms in Chinese expectant mother (β = -0.0126, < 0.001).

Conclusion: Pregnant women with strong resilience were less influenced by perceived stress than those with poor resilience. The findings of this study might give empirical proof that health care professionals should identify the relevance of reducing perceived stress in pregnant women with poor resilience and provide better treatment and support when necessary.
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http://dx.doi.org/10.3389/fpsyt.2022.856627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092980PMC
April 2022

Recent advances on endogenous gasotransmitters in inflammatory dermatological disorders.

J Adv Res 2022 05 1;38:261-274. Epub 2021 Sep 1.

Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.

Background: Endogenous gasotransmitters are small gaseous mediators that can be generated endogenously by mammalian organisms. The dysregulation of the gasotransmitter system is associated with numerous disorders ranging from inflammatory diseases to cancers. However, the relevance of these endogenous gasotransmitters, prodrug donors and inhibitors in inflammatory dermatological disorders has not yet been thoroughly reviewed and discussed.

Aim Of Review: This review discusses the recent progress and will provide perspectives on endogenous gasotransmitters in the context of inflammatory dermatological disorders.

Key Scientific Concepts Of Review: Endogenous gasotransmitters nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (HS) are signaling molecules that regulate several physiological and pathological processes. In addition, sulfur dioxide (SO₂), methane (CH), hydrogen gas (H), ammonia (NH), and carbon dioxide (CO) can also be generated endogenously and may take part in physiological and pathological processes. These signaling molecules regulate inflammation, vasodilation, and oxidative stress, offering therapeutic potential and attracting interest in the field of inflammatory dermatological disorders including psoriasis, atopic dermatitis, acne, rosacea, and chronic skin ulcers. The development of effective gas donors and inhibitors is a promising alternative to treat inflammatory dermatological disorders with controllable and precise delivery in the future.
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http://dx.doi.org/10.1016/j.jare.2021.08.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091779PMC
May 2022

Occurrence, source, and ecological risk assessment of organochlorine pesticides and polychlorinated biphenyls in the water-sediment system of Hangzhou Bay and East China Sea.

Mar Pollut Bull 2022 May 11;179:113735. Epub 2022 May 11.

Ningbo Academy of Oceanology and Fisheries, Ningbo, 315042, PR China.

The pollution characteristics, potential sources, and potential ecological risk of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) were investigated in the Hangzhou Bay (HZB) and East China Sea (ECS). Total OCPs concentration ranged from 2.62 to 102.07 ng/L and 4.41 to 75.79 μg/kg in the seawater and sediment samples, with PCBs concentration in the range of 0.40-51.75 ng/L and 0.80-45.54 μg/kg, respectively. The OCPs were positively correlated with nutrients, whereas PCBs presented a negative correlation. The newly imported dichlorodiphenyltrichloroethane (DDT) in HZB is mainly the mixing of technical DDT and dicofol sources. The PCB source composition is more likely related to the mixture of Kanechlor 300, 400, Aroclor 1016, 1242, and Aroclor 1248. Risk assessment results indicate that OCPs posed low risk in seawater. The potential risk of DDTs in the sediments is a cause of concern.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113735DOI Listing
May 2022

Incidence and Risk Factors for Cerebrovascular-Specific Mortality in Patients with Colorectal Cancer: A Registry-Based Cohort Study Involving 563,298 Patients.

Cancers (Basel) 2022 Apr 19;14(9). Epub 2022 Apr 19.

Department of Spine Surgery, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China.

Background: Colorectal cancer (CRC) is one of the most prevalent diseases and the second leading cause of death worldwide. However, the relationship between CRC and cerebrovascular-specific mortality (CVSM) remains elusive, and less is known about the influencing factors associated with CVSM in CRC. Here, we aimed to analyze the incidence as well as the risk factors of CVSM in CRC.

Methods: Patients with a primary CRC diagnosed between 1973 and 2015 were identified from the Surveillance Epidemiology and End Results database, with follow-up data available until 31 December 2016. Conditional standardized mortality ratios were calculated to compare the incidence of CVSM between CRC patients and the general U.S.

Population: Univariate and multivariate survival analyses with a competing risk model were used to interrogate the risk factors for CVSM.

Results: A total of 563,298 CRC individuals were included. The CVSM in CRC patients was significantly higher than the general population in all age subgroups. Among the competing causes of death in patients, the cumulative mortality caused by cerebrovascular-specific diseases steadily increased during the study period. While age, surgery, other/unknown race and tumors located at the transverse colon positively influenced CVSM on both univariate and multivariate analyses, male patients and those who had radiotherapy, chemotherapy, a more recent year (2001-2015) of diagnosis, a grade II or III CRC, rectal cancer, or multiple primary or distant tumors experienced a lower risk of CVSM.

Interpretation: Our data suggest a potential role for CRC in the incidence of CVSM and also identify several significant predictors of CVSM that may be helpful for risk stratification and the therapeutic optimization of cerebrovascular-specific diseases in CRC patients.
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http://dx.doi.org/10.3390/cancers14092053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105882PMC
April 2022

Multi-ancestry genetic study of type 2 diabetes highlights the power of diverse populations for discovery and translation.

Nat Genet 2022 May 12;54(5):560-572. Epub 2022 May 12.

Public Health Informatics Unit, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.

We assembled an ancestrally diverse collection of genome-wide association studies (GWAS) of type 2 diabetes (T2D) in 180,834 affected individuals and 1,159,055 controls (48.9% non-European descent) through the Diabetes Meta-Analysis of Trans-Ethnic association studies (DIAMANTE) Consortium. Multi-ancestry GWAS meta-analysis identified 237 loci attaining stringent genome-wide significance (P < 5 × 10), which were delineated to 338 distinct association signals. Fine-mapping of these signals was enhanced by the increased sample size and expanded population diversity of the multi-ancestry meta-analysis, which localized 54.4% of T2D associations to a single variant with >50% posterior probability. This improved fine-mapping enabled systematic assessment of candidate causal genes and molecular mechanisms through which T2D associations are mediated, laying the foundations for functional investigations. Multi-ancestry genetic risk scores enhanced transferability of T2D prediction across diverse populations. Our study provides a step toward more effective clinical translation of T2D GWAS to improve global health for all, irrespective of genetic background.
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http://dx.doi.org/10.1038/s41588-022-01058-3DOI Listing
May 2022

Novel MDM2 Inhibitor XR-2 Exerts Potent Anti-Tumor Efficacy and Overcomes Enzalutamide Resistance in Prostate Cancer.

Front Pharmacol 2022 25;13:871259. Epub 2022 Apr 25.

Department of Urology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

The inactivation of tumor-suppressor p53 plays an important role in second generation anti-androgens (SGAs) drug resistance and neuroendocrine differentiation in castration-resistant prostate cancer (CRPC). The reactivation of p53 by blocking the MDM2-p53 interaction represents an attractive therapeutic remedy in cancers with wild-type or functional p53. Whether MDM2-p53 inhibitor could overcome SGAs drug resistance in CRPC is still needed further research. Here, we investigated the anti-tumor efficacy and mechanisms of a novel MDM2-p53 inhibitor XR-2 in CRPC. To investigate the functions and mechanisms of XR-2 in prostate cancer, and biofunctional assays were performed. Western blot and qRT-PCR assay were performed to detect the protein and mRNA expression levels of indicated genes. CCK8, colony formation, flow cytometry and senescence assays were performed for cell function identifications. RNA-sequencing and bioinformatics analysis were mainly used to identify the influence of XR-2 on prostate cancer cells transcriptome. Subcutaneous 22Rv1 derived xenografts mice model was used to investigate the anti-tumor activity of XR-2. In addition, the broad-spectrum anti-tumor activities of XR-2 were evaluated by different xenografts mice models. XR-2 could directly bind to MDM2, potently reactivate the p53 pathway and thus induce cell cycle arrest and apoptosis in wild-type p53 CRPC cell lines. XR-2 also suppresses the AR pathway as p53 regulates AR transcription inhibition and MDM2 participates in AR degradation. As a result, XR-2 efficiently inhibited CRPC cell viability, showed a synergistic effect with enzalutamide and overcame enzalutamide resistance both and . Moreover, results illustrated that XR-2 possesses broad-spectrum anti-tumor activities with favourable safety. MDM2-p53 inhibitor (XR-2) possesses potently prostate cancer progresses inhibition activity both and . XR-2 shows a synergistic effect with enzalutamide and overcomes enzalutamide resistance.
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http://dx.doi.org/10.3389/fphar.2022.871259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081362PMC
April 2022

Genomic and Transcriptomic Analyses Reveal Pathways and Genes Associated With Brittle Stalk Phenotype in Maize.

Front Plant Sci 2022 25;13:849421. Epub 2022 Apr 25.

Maize Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling, China.

The mechanical strength of the stalk affects the lodging resistance and digestibility of the stalk in maize. The molecular mechanisms regulating the brittleness of stalks in maize remain undefined. In this study, we constructed the maize brittle stalk mutant () by crossing the W22:Mu line with the Zheng 58 line. The brittle phenotype of the mutant existed in all of the plant organs after the five-leaf stage. Compared to wild-type (WT) plants, the sclerenchyma cells of stalks had a looser cell arrangement and thinner cell wall. Determination of cell wall composition showed that obvious differences in cellulose content, lignin content, starch content, and total soluble sugar were found between and WT stalks. Furthermore, we identified 226 differentially expressed genes (DEGs), with 164 genes significantly upregulated and 62 genes significantly downregulated in RNA-seq analysis. Some pathways related to cellulose and lignin synthesis, such as endocytosis and glycosylphosphatidylinositol (GPI)-anchored biosynthesis, were identified by the Kyoto Encyclopedia of Gene and Genomes (KEGG) and gene ontology (GO) analysis. In bulked-segregant sequence analysis (BSA-seq), we detected 2,931,692 high-quality Single Nucleotide Polymorphisms (SNPs) and identified five overlapped regions (11.2 Mb) containing 17 candidate genes with missense mutations or premature termination codons using the SNP-index methods. Some genes were involved in the cellulose synthesis-related genes such as ENTH/ANTH/VHS superfamily protein gene (endocytosis-related gene) and the lignin synthesis-related genes such as the cytochrome p450 gene. Some of these candidate genes identified from BSA-seq also existed with differential expression in RNA-seq analysis. These findings increase our understanding of the molecular mechanisms regulating the brittle stalk phenotype in maize.
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http://dx.doi.org/10.3389/fpls.2022.849421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083323PMC
April 2022

Decaprenyl Diphosphate Synthase Subunit 1 (PDSS1): A Potential Prognostic Biomarker and Immunotherapy-Target for Hepatocellular Carcinoma.

Cancer Manag Res 2022 3;14:1627-1639. Epub 2022 May 3.

Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, Guangdong Province, 510630, People's Republic of China.

Purpose: PDSS1 (decaprenyl diphosphate synthase subunit 1) plays an important role in the progression of several types of tumor. However, the biological functions of PDSS1 remain unclear in patients with hepatocellular carcinoma (HCC). In this study, We attempted to determine the role of PDSS1 in predicting the survival and efficacy of immunotherapy for HCC patients.

Methods: We analyzed the expression of PDSS1 in pan-cancer by Tumor Immune Estimation Resource (TIMER) and UALCAN database. Next, we investigated the correlations between the expression and potential prognostic value of PDSS1 in pan-cancer by Gene Expression Profiling Interactive Analysis (GEPIA) and Kaplan-Meier plotter and further confirmed our finding in our study of 139 patients with HCC. Furthermore, we correlated expression patterns with the presence of immune cell infiltrates and immune regulatory molecules in HCC tissue by TIMER. Finally, its potential immune-related mechanism was explored by Gene Set Enrichment Analysis (GSEA).

Results: Multiple datasets demonstrate that PDSS1 is up-regulated in HCC tissues compared with adjacent tissues, which was validated at mRNA (databases) and protein levels (our cohort). Patients with higher PDSS1 expression had shorter overall survival and relapse-free survival. In addition, PDSS1 expression was positively related to early recurrence and served as an independent poor prognostic factor for HCC. Patients with higher PDSS1 expression had lower CD8+ T cells in HCC tissue, and PDSS1 deteriorates T cell exhaustion by promoting T cell surface inhibitory receptors' secretion and immunosuppressive cell proliferation. Furthermore, PDSS1 was positively correlated with the WNT, TGFβ, VEGF, and other signaling pathways in HCC.

Conclusion: PDSS1 is a potential prognostic biomarker and immunotherapy target for hepatocellular carcinoma.
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http://dx.doi.org/10.2147/CMAR.S364346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078875PMC
May 2022

RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.

Cell Death Differ 2022 May 9. Epub 2022 May 9.

Key Laboratory of Cancer and Microbiome, State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Runt-related transcription factor 2 (RUNX2) is an osteogenesis-related transcription factor that has emerged as a prominent transcription repressing factor in carcinogenesis. However, the role of RUNX2 in breast cancer metastasis remains poorly understood. Here, we show that RUNX2 recruits the metastasis-associated 1 (MTA1)/NuRD and the Cullin 4B (CUL4B)-Ring E3 ligase (CRL4B) complex to form a transcriptional-repressive complex, which catalyzes the histone deacetylation and ubiquitylation. Genome-wide analysis of the RUNX2/NuRD(MTA1)/CRL4B complex targets identified a cohort of genes including peroxisome proliferator-activated receptor alpha (PPARα) and superoxide dismutase 2 (SOD2), which are critically involved in cell growth, epithelial-to-mesenchymal transition (EMT) and invasion. We demonstrate that the RUNX2/NuRD(MTA1)/CRL4B complex promotes the proliferation, invasion, tumorigenesis, bone metastasis, cancer stemness of breast cancer in vitro and in vivo. Strikingly, RUNX2 expression is upregulated in multiple human carcinomas, including breast cancer. Our study suggests that RUNX2 is a promising potential target for the future treatment strategies of breast cancer.
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http://dx.doi.org/10.1038/s41418-022-01010-2DOI Listing
May 2022

TREM-2 mediates dendritic cell-induced NO to suppress Th17 activation and ameliorate chronic kidney diseases.

J Mol Med (Berl) 2022 May 9. Epub 2022 May 9.

Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St, Beitou District, Taipei, 11221, Taiwan.

Chronic kidney disease (CKD) is a global public health issue. CKD is caused by the infiltration of various myeloid cell types into renal tissue, resulting in renal fibrosis and tubular atrophy. Unilateral ureteral obstruction (UUO) surgery in mice is a model of CKD and characterized by high expression of the anti-inflammatory receptor, Triggering receptor expressed on myeloid cells 2 (TREM-2), on myeloid cells in affected kidneys. Here, we show that iNOS expression and nitric oxide (NO) induction were decreased in Trem-2 bone marrow-derived DCs (BMDCs) and in Trem-2 knockdown DC2.4 cells stimulated in vitro with LPS. The nitration of RORγt was decreased in T cells co-cultured with LPS-stimulated Trem-2 BMDCs, enhancing IL-17 production. UUO-treated Trem-2 mice displayed aggravated renal pathogenesis accompanied by greater neutrophil infiltration and enhanced Th17 cells differentiation, phenotypes that could be rescued by the administration of L-arginine (a biological precursor of NO). Our data identify a key mechanism underlying TREM-2-mediated NO to modulate the cellular crosstalk between dendritic cells, Th17, and neutrophils. Furthermore, we also reveal TREM-2 as a potential novel target for the development of anti-inflammatory drugs in CKD treatment. KEY MESSAGES: The expression of TREM-2 is increased in nephritis TREM-2 DCs maintain NO production to negatively regulate Th17 differentiation The severe pathologies of nephritis can be rescued by L-arginine supplementation.
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http://dx.doi.org/10.1007/s00109-022-02201-7DOI Listing
May 2022

KRT17 Promotes the Activation of HSCs via EMT in Liver Fibrosis.

J Clin Transl Hepatol 2022 Apr 8;10(2):207-218. Epub 2021 Jul 8.

Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, China.

Background And Aims: Although activation of hepatic stellate cells (HSCs) plays a central role in the development of liver fibrosis, the mechanism underlying the activation of HSCs remains unclear. Keratin 17 (KRT17), a member of the intermediate filament family, can regulate tumor cell proliferation and migration. The current study aimed to elucidate the role of KRT17 in the activation of HSCs and the mechanisms underlying liver fibrosis.

Methods: The expression of KRT17 was determined using immunohistochemistry in tissue microarray. Western blotting and qRT-PCR assays were used to determine the KRT17 expression in fibrotic liver tissues obtained from human subjects and mice. LX-2 cells were treated with TGF-β1 recombinant protein and adipocyte differentiation mixture (MDI) mix to induce and reverse LX-2 cell activation, respectively, in order to explore the correlation between KRT17 and HSC activation. Additionally, cell proliferation and migration abilities of LX-2 cells transfected with KRT17-overexpressing plasmid or small interfering RNA were determined using CCK-8, flow cytometry, Transwell, and wound healing assays. Finally, rescue assay was used to explore the role of KRT17 in HSC activation and epithelial-mesenchymal transition (EMT).

Results: The expression of KRT17 was higher in the human and mouse fibrotic liver tissues than in healthy liver tissues, and it was positively correlated with HSC activation. Upregulated KRT17 enhanced proliferation, migration, HSC activation and EMT in LX-2 cells, while knockdown of KRT17 reversed these effects. TGF-β1 recombinant protein accelerated KRT17-mediated EMT, HSC activation and proliferation, while TGF-β1 inhibitor counteracted the effect of KRT17 .

Conclusions: KRT17 promoted HSC activation, proliferation and EMT in hepatic fibrosis probably via TGF-β1 signaling, and KRT17 might serve as a therapeutic target for the treatment of liver fibrosis.
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http://dx.doi.org/10.14218/JCTH.2021.00101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039702PMC
April 2022

Simultaneous quantification of plasma immunoglobulin subclasses for assessment of maternal and fetal immune response during pregnancy.

J Chromatogr A 2022 Apr 29;1673:463096. Epub 2022 Apr 29.

School of Environment, Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.

Measurement of immunoglobulin subclasses is a useful tool for exploring humoral immune deficiency in the presence of total immunoglobulins within reference intervals. Conventional methods for immunoglobulin measurement are mostly immunoassays, which are of low throughput and laborious to run multiple immunoglobulin subclass tests. Liquid chromatography-mass spectrometry (LC-MS) has emerged as a promising technology for the measurement of protein biomarkers in biological matrices, owing to its high specificity, selectivity, multiplexing, and wide dynamic range. In fully taking these advantages, we developed here a LC-MS based methodology for simultaneous quantitation of the primary immunoglobulin isotypes (IgG, IgA, IgM) and their subclasses in human plasma. Method validation demonstrated that the proposed method showed good linearity with R > 0.99, high precision with coefficients of variation for inter- and intra-assay less than 15%, as well as high accuracy with relative errors less than 8.7%. The developed method was further applied to maternal and cord blood collected at delivery for assessment of maternal and fetal immune status. The immunoglobulin profiles and the features of transplacental transport of maternal immunoglobulin subclasses were comparable to the findings from previous reports, further demonstrating the reliability of this method. Therefore, our method provides a competitive approach to high-throughput detection of multiple immunoglobulin subclasses for biomonitoring or epidemiological studies.
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http://dx.doi.org/10.1016/j.chroma.2022.463096DOI Listing
April 2022

Filtering electrons by mode coupling in finite semiconductor superlattices.

Sci Rep 2022 May 7;12(1):7502. Epub 2022 May 7.

Frontiers Science Center for Flexible Electronics (FSCFE), Shaanxi Institute of Flexible Electronics (SIFE) & Shaanxi Institute of Biomedical Materials and Engineering (SIBME), Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, 710072, China.

Electron transmission through semiconductor superlattices is studied with transfer matrix method and resonance theory. The formation of electron band-pass transmission is ascribed to the coupling of different modes in those semiconductor superlattices with the symmetric unit cell. Upon Fabry-Pérot resonance condition, Bloch modes and two other resonant modes are identified to be related to the nature of the superlattice and its unit cell, respectively. The bands related to the unit cell and the superlattice overlap spontaneously in the tunneling region due to the shared wells, and the coupling of perfect resonances results in the band-pass tunneling. Our findings provide a promising way to study electronic systems with more complicated superlattices or even optical systems with photonic crystals.
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http://dx.doi.org/10.1038/s41598-022-11449-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079071PMC
May 2022

Early Hyperchloremia is Independently Associated with Death or Disability in Patients with Intracerebral Hemorrhage.

Neurocrit Care 2022 May 5. Epub 2022 May 5.

Department of Cerebrovascular Medicine, National Cerebral and Cardiovascular Center, Suita, Japan.

Background: On the basis of increased mortality associated with hyperchloremia among critically ill patients, we investigated the effect of occurrence of early hyperchloremia on death or disability at 90 days in patients with intracerebral hemorrhage (ICH).

Methods: We analyzed the data from Antihypertensive Treatment of Cerebral Hemorrhage 2 trial, which recruited patients with spontaneous ICH within 4.5 h of symptom onset. Patients with increased serum chloride levels (110 mmol/L or greater) at either baseline or 24, 48, or 72 h after randomization were identified. We further graded hyperchloremia into one occurrence or two or more occurrences within the first 72 h. Two logistic regression analyses were performed to determine the effects of hyperchloremia on (1) death within 90 days and (2) death or disability at 90 days after adjustment for potential confounders.

Results: Among the total of 1,000 patients analyzed, hyperchloremia within 72 h was seen in 114 patients with one occurrence and in 154 patients with two or more occurrences. Patients with one occurrence of hyperchloremia (odds ratio [OR] 2.5, 95% confidence interval [CI] 1.1-5.5) and those with two or more occurrences (OR 2.6, 95% CI 1.3-5.0) had significantly higher odds of death within 90 days after adjustment for age, race and ethnicity, National Institutes of Health Stroke Scale score strata, hematoma volume, presence or absence of intraventricular hemorrhage, cigarette smoking, previous stroke, and maximum hourly dose of nicardipine. Patients with two or more occurrences of hyperchloremia (OR 3.4, 95% CI 2.1-5.6) had significantly higher odds of death or disability at 90 days compared with patients without hyperchloremia after adjustment for the abovementioned potential confounders.

Conclusions: The independent association between hyperchloremia and death or disability at 90 days suggests that avoidance of hyperchloremia may reduce the observed death or disability in patients with ICH.

Clinical Trial Registration: ClinicalTrials.gov: NCT01176565.
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http://dx.doi.org/10.1007/s12028-022-01514-2DOI Listing
May 2022

Thermal Stability and Kinetics of Single [email protected] Particles.

ACS Appl Mater Interfaces 2022 May 5;14(19):22643-22649. Epub 2022 May 5.

National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang 621010, P. R. China.

Thermogravimetric analysis (TGA) is a key material characterization method for studying the thermal stability and thermochemical process. However, the common TGA for bulk samples lacks sufficient spatial information, which blurs the intrinsic thermal decomposition characteristic and limits the understanding of the structure-performance relationship. Here, we report a dark-field microscope (DFM) method for studying thermal desorption process of I from I-loaded zeolitic imidazolate framework-8 ([email protected]). Because of the high spatial resolution, DFM enables the imaging and tracking of the local mass loss of I in single [email protected] particles at different reaction temperatures. We obtain from the DFM images the single-particle thermogravimetric and differential thermogravimetric curves to evaluate the inherent thermal stability of single [email protected] particles. We also find the heterogeneous thermal decomposition property among different [email protected] particles. Furthermore, we demonstrate the capacity of DFM to quantitatively determine thermal kinetics parameters such as the diffusion coefficient and activation energy of I in individual and multiple ZIF-8 particles. These useful results are essential for developing high-efficient porous adsorbents for the capture of I.
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http://dx.doi.org/10.1021/acsami.2c04922DOI Listing
May 2022

Chlorine substituted N-heteroacene analogues acting as organic semiconductors for solution-processed n-type organic field-effect transistors.

Chemistry 2022 May 4. Epub 2022 May 4.

Northwestern Polytechnical University, Northwestern Polytechnical University, CHINA.

High performance solution processable n-type organic semiconductor is an essential element to realize low-cost, all organic and flexible composite logic circuits. In the design of n-type semiconducting materials, tuning the LUMO level of compounds is a key point. As a strong electron withdrawing unit, the introduction of chlorine atom into the chemical structure can increase the electron affinity of the material and reduce the LUMO energy level. Here, a series chlorine substituted N-heteroacene analogues of 6,7,8,9-tetrachloro-4,11-bis(4-((2-ethylhexyl)oxy)phenyl)-[1,2,5]thiadiazolo[3,4-b]phenazine (O4Cl), 6,7,8,9-tetrachloro-4,11-bis(4-((2-ethylhexyl)thio)phenyl)-[1,2,5]thiadiazolo[3,4-b]phenazine (S4Cl), 1,2,3,4,8,9,10,11-octachloro-6,13-bis(4-((2-ethylhexyl)oxy)phenyl)quinoxalino[2,3-b]phenazine (8Cl) and 12Cl have been synthesized and characterized. Solution-processed organic field-effect transistors (OFETs) based on these four compounds exhibit good electron mobilities of 0.04 cm 2 V -1 s -1 , 0.01 cm 2 V -1 s -1 , 2 × 10 -3 cm 2 V -1 s -1 and 3 × 10 -3 cm 2 V -1 s -1 , respectively, under ambient conditions. The results suggest that these chlorine substituted π-conjugated N-heteroacene analogues are promising n-type semiconductors in OFET applications.
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http://dx.doi.org/10.1002/chem.202201176DOI Listing
May 2022

Enhancing COVID-19 Epidemics Forecasting Accuracy by Combining Real-time and Historical Data from Multiple Internet-based Sources: Analysis of Social Media Data, Online News Articles, and Search Queries.

JMIR Public Health Surveill 2022 May 3. Epub 2022 May 3.

School of Management, Xi'an Jiaotong University, Xi'an, CN.

Background: The SARS-COV-2 virus and its variants pose extraordinary challenges for public health worldwide. Timely and accurate forecasting of the COVID-19 epidemic is the key to sustaining interventions and policies and efficient resources allocation. Internet-based data sources have shown great potential to supplement traditional infectious disease surveillance, and the combination of different Internet-based data sources has shown greater power to enhance epidemic forecasting accuracy than using a single Internet-based data source. However, existing methods incorporating multiple Internet-based data sources only used real-time data from these sources as exogenous inputs but did not take all the historical data into account. Moreover, the predictive power of different Internet-based data sources in providing early warning for COVID-19 outbreaks has not been fully explored.

Objective: The main aim of our study is to explore whether combining real-time and historical data from multiple Internet-based sources could improve the COVID-19 forecasting accuracy over the existing baseline models. A secondary aim is to explore the COVID-19 forecasting timeliness based on different Internet-based data sources.

Methods: We first used core terms and symptoms-related keywords-based methods to extract COVID-19 related Internet-based data from December 21, 2019, to February 29, 2020. The Internet-based data we explored included 90,493,912 online news articles, 37,401,900 microblogs, and all the Baidu search query data during that period. We then proposed an autoregressive model with exogenous inputs, incorporating the real-time and historical data from multiple Internet-based sources. Our proposed model was compared with baseline models, and all the models were tested during the first wave of COVID-19 epidemics in Hubei province and the rest of mainland China separately. We also used the lagged Pearson correlations for the COVID-19 forecasting timeliness analysis.

Results: Our proposed model achieved the highest accuracy in all the five accuracy measures, compared with all the baseline models of both Hubei province and the rest of mainland China. In the mainland China except for Hubei, the COVID-19 epidemics forecasting accuracy differences between our proposed model (model i) and all the other baseline models were statistically significant (model 1, t=-8.722, P<.001; model 2, t=-5.000, P<.001, model 3, t=-1.882, P =0.063, model 4, t=-4.644, P<.001; model 5, t=-4.488, P<.001). In Hubei province, our proposed model's forecasting accuracy improved significantly compared with the baseline model using historical COVID-19 new confirmed case counts only (model 1, t=-1.732, P=0.086). Our results also showed that Internet-based sources could provide a 2-6 days earlier warning for COVID-19 outbreaks.

Conclusions: Our approach incorporating real-time and historical data from multiple Internet-based sources could improve forecasting accuracy for COVID-19 epidemics and its variants, which may help improve public health agencies' interventions and resources allocation in mitigating and controlling new waves of COVID-19 or other relevant epidemics.

Clinicaltrial:
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http://dx.doi.org/10.2196/35266DOI Listing
May 2022

Stimulating and Manipulating Robust Circularly Polarized Photoluminescence in Achiral Hybrid Perovskites.

Nano Lett 2022 May 4. Epub 2022 May 4.

Key Laboratory of Flexible Electronics and Institute of Advanced Materials, School of Physical and Mathematical Sciences, Nanjing Tech University, Nanjing 211816, China.

Circularly polarized light (CPL) is essential for optoelectronic and chiro-spintronic applications. Hybrid perovskites, as star optoelectronic materials, have demonstrated CPL activity, which is, however, mostly limited to chiral perovskites. Here, we develop a simple, general, and efficient strategy to stimulate CPL activity in achiral perovskites, which possess rich species, efficient luminescence, and tunable bandgaps. With the formation of van der Waals heterojunctions between chiral and achiral perovskites, a nonequilibrium spin population and thus CPL activity are realized in achiral perovskites by receiving spin-polarized electrons from chiral perovskites. The polarization degree of room-temperature CPL in achiral perovskites is at least one order of magnitude higher than in chiral ones. The CPL polarization degree and emission wavelengths of achiral perovskites can be flexibly designed by tuning chemical compositions, operating temperature, or excitation wavelengths. We anticipate that unlimited types of achiral perovskites can be endowed with CPL activity, benefiting their applications in integrated CPL sources and detectors.
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http://dx.doi.org/10.1021/acs.nanolett.2c00482DOI Listing
May 2022

Correlation of KMT2 family mutations with molecular characteristics and prognosis in colorectal cancer.

Int J Biol Markers 2022 May 3:3936155221095574. Epub 2022 May 3.

Department of Colorectal & Anal Surgery, 117742The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Lysine methyltransferase 2 (KMT2) family proteins methylate lysine 4 on histone H3 (H3K4) to promote genome accessibility and transcription. Dysregulation or mutation of KMT2 family have been observed frequently in various types of human cancers. Colorectal cancer is the third most common cancer worldwide. However, few studies have evaluated the role of KMT2 family mutations in colorectal cancer. The present study aimed to explore the impact of KMT2 family mutations on clinicopathological, molecular characteristics and prognosis in colorectal cancer.

Methods: A total of 316 colorectal cancer patients were enrolled; tumor tissue and matched peripheral blood samples were collected and subjected to targeted sequencing with a panel of 1021 cancer-related genes. The association of clinical pathological features and molecular characteristics in patients were then analyzed. The cBioPortal dataset was used for investigating the KMT2 family mutations data and their correlation with clinical outcomes.

Results: The overall mutation frequencies of were 9.5%, 0.5%, 13%, and 13%, respectively, which were more often present at right-sided primary and earlier stage tumors. mutations are associated with enhanced genomic instability, including a higher level of microsatellite instability (MSI-H) and tumor mutational burden (TMB-H). In addition, our results highlight the co-occurring gene mutations within the Wnt signaling, ERBB2/4, TGF-β superfamily pathway, and PI-3-kinase pathway in -mutant colorectal cancer. KMT2 family mutations were predictive biomarker for better overall survival in metastatic colorectal cancer.

Conclusions: Collectively, we identified that KMT2 family mutations were correlated with higher-TMB and higher-MSI, thus resulting in a better outcome for colorectal cancer patients.
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http://dx.doi.org/10.1177/03936155221095574DOI Listing
May 2022

Neuropeptide FF-related gene in fish (Larimichthys polyactis): identification, characterization, and potential anti-inflammatory function.

Mol Biol Rep 2022 May 3. Epub 2022 May 3.

Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Background: Neuropeptide FF (NPFF), an octapeptide of the RFamide-related peptides (FaRPs), is involved in regulatory function in various biological processes. The regulatory role of NPFF in the immune and inflammatory response was currently being revealed.

Methods: Neuropeptide FF-related gene (termed LpNPFF) and its two receptors, NPFF receptor 1 (LpNPFFR1) and NPFF receptor 2 (LpNPFFR2) were identified by PCR and Semi-quantitative RT-PCR assay. Effect of LpNPFF on the production of nitric oxide (NO) in macrophage RAW264.7 cell was divided into PBS group, lipopolysaccharide (LPS) group, LPS treated with LpNPFF group, and LPS treated with LpNPFF and receptor antagonist RF9 group. Then specimens were measured by color reaction at 570 nm absorbance value.

Results: Sequence analysis showed that LpNPFF cDNA consists of 835 nucleotides with a 5'- untranslated region (UTR) of 150 base pair (bp), an open reading frame (ORF) of 384 bp and a 3'-UTR of 300 bp (Accession No. MT012894). The ORF encodes 127 amino acid (aa) residues with a hydrophobic signal peptide at N-terminus and two presumptive peptides with -PQRFa structure, LpNPFF (1) and LpNPFF (2). LpNPFFR1 and LpNPFFR2 encode 427 and 444 aa residues respectively, which both have seven hydrophobic TMDs and identified as G protein coupled receptors (GPCRs). Results of tissue distribution showed that LpNPFF and receptors were highly expressed in the brain and gonad. Furtherly, in vitro assay found LpNPFF could inhibit NO production in RAW 264.7 macrophages under inflammatory stress with LPS, while its receptor antagonist RF9 caused the evoke of NO generation.

Conclusions: These results contribute to the further study of neuropeptide evolution in marine organisms, and also provide a new research idea for exploring the related functions of NPFF gene.
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http://dx.doi.org/10.1007/s11033-022-07447-5DOI Listing
May 2022
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