Publications by authors named "Wei Hu"

2,373 Publications

  • Page 1 of 1

Differentiation and Maturation Effect of All-trans Retinoic Acid on Cultured Fetal RPE and Stem Cell-Derived RPE Cells for Cell-Based Therapy.

Curr Eye Res 2022 Jun 28:1-34. Epub 2022 Jun 28.

Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang, Liaoning Province 110001 PR China.

Purpose: Clinical trials using fetal retinal pigment epithelium (fRPE), human embryonic stem cell (hESC)-derived RPE, or human induced pluripotent stem cell (hiPSC)-derived RPE for cell-based therapy for degenerative retinal diseases have been carried out. We investigated the culture-induced changes in passaged fRPE, hESC-RPE and hiPSC-RPE cells and explored the differentiation and maturation effect of all-trans retinoic acid (ATRA) on cells for manufacturing and screening high quality RPE cells for clinical transplantation.

Methods: RPE cell lines were set up and the culture-induced changes in subsequent passages caused by manipulating plating density, dissociation method and repeated passaging were studied by microscope, real-time quantitative PCR, western blot and immunofluorescent assays. Gene and protein expression and functional characteristics of RPE cells incubated with ATRA were evaluated.

Results: Compared with fRPE, hESC-RPE and hiPSC-RPE showed decreased gene and protein expression of RPE markers. RPE cells underwent mesenchymal changes showing increased expression of mesenchymal markers including a-SMA, N-cadherin, fibronectin and decreased expression of RPE markers including RPE65, E-cadherin and ZO-1, as a subsequence of low plating density, inappropriate dissociated method, and repeated passaging. RPE cells treated by ATRA showed increased expression of RPE markers and increased expression of negative complement regulatory proteins (CRPs), and increased transepithelial resistance as well.

Conclusions: Differences in protein and gene expression among three RPE types exist. ATRA can increase RPE markers, CRPs gene expression in fRPE and stem cell-derived RPE. These can be used to guide the standard of screening RPE cells for clinical translational cell therapy.
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http://dx.doi.org/10.1080/02713683.2022.2079144DOI Listing
June 2022

A Prospective, Multicenter, Single-Group Target-Value Clinical Trial to Evaluate the Safety and Efficacy of a Large Bore Aspiration Catheter System for the Endovascular Treatment of Acute Ischemic Stroke.

Front Neurol 2022 9;13:864563. Epub 2022 Jun 9.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objective: This study was conducted to determine the safety and efficacy of the Esperance Distal Access Catheter (0.071"), a novel large bore aspiration catheter in treating acute ischemic stroke (AIS) with large vessel occlusion (LVO).

Methods: A prospective multicenter clinical trial involving 15 stroke centers was performed. Baseline characteristics, procedural data, and angiographic and clinical outcomes of all acute stroke procedures (from May 2020 to March 2021) using the novel large bore aspiration catheter were analyzed.

Results: During the study period, 160 consecutive patients were recruited. The mean age and median baseline NIHSS were 65.4 years and 16, respectively. Successful reperfusion was achieved in 147 (91.9%) cases [at least modified Thrombolysis in Cerebral Infarction (mTICI) 2b] with aspiration alone, with complete reperfusion (mTICI 3) in 94 (58.8%) cases. Successful/complete first pass reperfusion was achieved in 104 (64.60%) cases, including mTICI 2b in 34 (21.1%) cases and mTICI 3 in 70 (43.5%) cases. The time from groin puncture to successful reperfusion was 44 (33, 62) min. There were 16 (9.9%) cases requiring rescue therapy using stent-retriever. Procedure-related complications included 2 (1.3%) cases of arterial perforation, 2 (1.3%) cases of arterial dissection, 12 (7.5%) cases of distal embolization, and 1(0.6%) case of puncture site infection. The rate of symptomatic intracranial hemorrhage (sICH) was 3.8% and mortality rate was 13.8%. A total of 99 (62.3%) cases had a favorable outcome (mRS 0-2) at 90 days.

Conclusions: In current practice, the first-line aspiration approach with the Esperance Distal Access Catheter is safe and efficacious. This device may achieve high reperfusion rates with lower instances of rescue stent retriever therapy.
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http://dx.doi.org/10.3389/fneur.2022.864563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218266PMC
June 2022

A Novel Rabies Vaccine Based on a Recombinant Bovine Herpes Virus Type 1 Expressing Rabies Virus Glycoprotein.

Front Microbiol 2022 8;13:931043. Epub 2022 Jun 8.

The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China.

Rabies is a highly prevalent zoonotic disease and a public health threat worldwide. Currently licensed rabies vaccines are effective but less is known which would protect cattle. This study describes the construction of a novel recombinant bovine herpes virus type I (BHV-1) expressing rabies virus glycoprotein (RABV G) instead of its gE glycoprotein (gE) by CRISPR-Cas9 and homologous recombination technology (BHV-1-ΔgE-G). Insertion of the RABV G gene is stable after 20 rounds of passaging and the recombinant virus replicates to high titers in MDBK cells. The RABV G expresses in the recombinant virus-infected cells and on the virion surface of BHV-1-ΔgE-G. One single immunization with BHV-1-ΔgE-G-activated dendritic cells (DCs) and B cells furthermore induced a protective immune response in mice against severe lethal challenge infection. A protective level of RABV-specific virus-neutralizing antibody (VNA) was detected in intramuscular immunized mice and cattle without any clinical symptoms. This research demonstrated that the BHV-1 vector-based RABV vaccine is a potential candidate for cattle.
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http://dx.doi.org/10.3389/fmicb.2022.931043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213812PMC
June 2022

Efficient phosphorus recovery as struvite by microbial electrolysis cell with stainless steel cathode: Struvite purity and experimental factors.

Sci Total Environ 2022 Jun 23:156914. Epub 2022 Jun 23.

Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China; Engineering Research Center for Water Pollution Source Control & Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address:

Phosphorus (P) recovery from waste streams is an essential choice due to the coming global P crisis. One promising solution is to recover P by microbial electrolysis cell (MEC). Both the P recovery effectiveness and product quality are of critical importance for application. In this study, a two-chamber MEC was constructed and the effects of applied voltage, NaAc concentration, Mg/P molar ratio, N/P molar ratio, and initial P concentration on P recovery and product purity were explored. The maximum P recovery efficiency of 99.64 % and crystal accumulation rate over 106.49 g/m-d were achieved. Struvite (MAP) was confirmed as the final recovered product and the purity obtained could reach up to 99.95 %. Besides, higher applied voltage, N/P molar ratio and initial P concentration could promote P recovery efficiency, while the purity of MAP showed correlation with applied voltage, Mg/P molar ratio, N/P molar ratio and initial P concentration. The correlation between NaAc concentration and both of the above was not very significant. A lower energy consumption of 4.1 kWh/kg P was observed at the maximum P recovery efficiency. In addition, the efficiency of P recovery from real wastewater also could reach nearly 88.25 %. These results highlight the promising potential of efficient phosphorus recovery from P-rich wastewater by MEC.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156914DOI Listing
June 2022

LINC00313 facilitates osteosarcoma carcinogenesis and metastasis through enhancing EZH2 mRNA stability and EZH2-mediated silence of PTEN expression.

Cell Mol Life Sci 2022 Jun 25;79(7):382. Epub 2022 Jun 25.

Department of Emergency, Qilu Hospital, Shandong University, Jinan, 250063, Shandong Province, China.

Background: Osteosarcoma is one of the five leading causes of cancer death among all pediatric malignancies. Recent advances in non-coding RNAs suggested that many long noncoding RNAs (lncRNAs) are dysregulated in cancer tissues and play important roles in carcinogenesis. We aimed to further explore the mechanisms of Long Intergenic Non-Protein Coding RNA 313 (LINC00313)-promoted malignant phenotypes of osteosarcoma.

Methods: The mRNA expressions were determined by quantitative real-time PCR. Protein levels were detected using Western blotting or immunohistochemistry staining. Protein binding to genomic DNA and RNA were measured using chromatin and RNA immunoprecipitation assay, respectively. CCK-8 and EdU incorporation assay were adopted to detect cell proliferation. Transwell assay was employed to assess the capacity of cell migration and invasion. The roles of LINC00313 and its target genes in tumorigenesis and metastasis of osteosarcoma were evaluated using subcutaneous xenograft models and tail vein inoculation models.

Results: LINC00313 was elevated in osteosarcoma tissues compared with adjacent normal tissues. Higher LINC00313 was associated with advanced grades of osteosarcoma. LINC00313 promoted cell proliferation, migration, invasion in vitro and tumor growth as well as metastasis in vivo through inhibiting PTEN expression to promote AKT phosphorylation. Mechanistically, LINC00313 favored the interaction between FUS and EZH2, leading to the prolonged half-life of EZH2 mRNA, thereby in turn up-regulating EZH2 proteins and increasing EZH2-mediated epigenetic silence of PTEN.

Conclusion: LINC00313 exerted oncogene-like actions through increasing EZH2 mRNA stability, leading to PTEN deficiency in osteosarcoma.
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http://dx.doi.org/10.1007/s00018-022-04376-1DOI Listing
June 2022

Antitumor Effect of Pseudolaric Acid B Involving Regulation of Notch1/Akt Signaling Response in Human Hepatoma Cell In Vitro.

Evid Based Complement Alternat Med 2022 14;2022:5353686. Epub 2022 Jun 14.

School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu, China.

Background: Liver cancer, particularly hepatocellular carcinoma (HCC), is the fourth leading cause of cancer-related death worldwide. Sorafenib is a crucial drug for the treatment of advanced HCC, but it is difficult to meet the challenge of increasing clinical demands due to its severe side effects and drug resistance. Hence, development of novel antitumor drugs is urged. Previous studies showed that pseudolaric acid B (PAB) could reduce the expression of protein kinase B (PKB/Akt), a downstream effector of Notch signaling, facilitating cell apoptosis in HCC. The disruption of Notch signaling was verified to exacerbate malignant progression and drug resistance, however, the antitumor effect of PAB on Notch signaling in HCC remains unclear. Thus, this study aims to investigate the anti-HCC effect of PAB in association with the regulation of Notch1/Akt signaling.

Methods: CCK-8 assay and transwell assay were used to examine the cell proliferation and invasion in Huh7 cells after treatment with PAB and a Notch inhibitor DAPT. Moreover, the cell cycle of Huh7 cells after treatment with PAB was analyzed using flow cytometry. Finally, the changes of Notch1, Jagged1, Hes1, and Akt expression at the protein and mRNA level in Notch1/Akt signaling in Huh7 cells after treatment with PAB and DAPT were analyzed using immunofluorescence assay and real-time qPCR.

Results: The proliferation rate of Huh7 cells exposed to PAB of 0.5, 1, 2, 4, 8, 10, 20, 40, 80, 100, and 200 mol/L revealed a time-and dose-dependent decrease showing cell cycle arrest at G2/M phase ( < 0.05). Furthermore, compared with the untreated group, at the concentration of 40 mol/L, the proliferation rate and invasion rate of Huh7 cells in PAB, DAPT, and PAB-DAPT combination (PAB + DAPT) group were significantly decreased ( < 0.05), but the PAB + DAPT showed no synergistic antiproliferation and anti-invasion effect in comparison with PAB treatment alone ( > 0.05). In addition, compared with the untreated group, PAB and DAPT alone significantly downregulated the expression of Notch1, Jagged1, Hes1, Akt mRNA, or/and protein in Huh7 cells ( < 0.05), but there was no significant difference in synergistic downregulated effect between the PAB + DAPT group and the PAB group ( > 0.05).

Conclusion: PAB can suppress proliferation and invasion of HCC cells through downregulating the expression of Notch1/Akt signaling protein and mRNA, and may be a potential novel antitumor drug candidate for the clinical treatment of HCC in the future.
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http://dx.doi.org/10.1155/2022/5353686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213129PMC
June 2022

Dual-color terahertz spatial light modulator for single-pixel imaging.

Light Sci Appl 2022 Jun 23;11(1):191. Epub 2022 Jun 23.

Research Institute of Superconductor Electronics (RISE), School of Electronic Science and Engineering, Nanjing University, Nanjing, 210023, China.

Spatial light modulators (SLM), capable of dynamically and spatially manipulating electromagnetic waves, have reshaped modern life in projection display and remote sensing. The progress of SLM will expedite next-generation communication and biomedical imaging in the terahertz (THz) range. However, most current THz SLMs are adapted from optical alternatives that still need improvement in terms of uniformity, speed, and bandwidth. Here, we designed, fabricated, and characterized an 8 × 8 THz SLM based on tunable liquid crystal metamaterial absorbers for THz single-pixel compressive imaging. We demonstrated dual-color compressive sensing (CS) imaging for dispersive objects utilizing the large frequency shift controlled by an external electric field. We developed auto-calibrated compressive sensing (ACS) algorithm to mitigate the impact of the spatially nonuniform THz incident beam and pixel modulation, which significantly improves the fidelity of reconstructed images. Furthermore, the complementary modulation at two absorption frequencies enables Hadamard masks with negative element values to be realized by frequency-switching, thereby halving the imaging time. The demonstrated imaging system paves a new route for THz single-pixel multispectral imaging with high reliability and low cost.
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http://dx.doi.org/10.1038/s41377-022-00879-5DOI Listing
June 2022

Noval porous phosphate-solubilizing bacteria beads loaded with BC/nZVI enhanced the transformation of lead fractions and its microecological regulation mechanism in soil.

J Hazard Mater 2022 Jun 17;437:129402. Epub 2022 Jun 17.

Innovation Academy for Green Manufacture, Key Laboratory of Green Process and Engineering, Beijing Engineering Research Centre of Process Pollution Control, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address:

Soil lead pollution becomes a serious environmental problem. Microbial remediation has received widespread attentions due to high efficiency and no secondary pollution. In this research, a noval porous spherical phosphate-solubilizing bacteria bead loaded with biochar/nZVI (Bio-bead) was used to passivate lead in soil, and the effects and microecological regulation mechanisms of this process were also investigated. The results showed that the pH, OM, and AP of soil in the Bio-bead group were increased and the ORP was decreased over time compared with the blank group. The proportion of stable (oxidizable and residue) fractions of lead in Bio-bead group (45%) was much higher than that of the blank group (35%). In addition, the result of microbial community structure showed that Bio-beads did not change the species of dominant bacterial, excepting the abundance of Pseudomonas increased significantly and the abundance of Sphingomonas reduced during remediation. Redundancy analysis showed that pH, OM, AP and the ratio of residual and oxidizable fractions lead in soil were positively correlated with the abundance of Pseudomonas, while ORP was negatively correlated with the abundance of Pseudomonas. These findings have proved that Bio-bead is a potential strategy for remediation of lead-contaminated soil even in complexed soils.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129402DOI Listing
June 2022

Flexible Control of Broadband Polarization in a Spintronic Terahertz Emitter Integrated with Liquid Crystal and Metasurface.

ACS Appl Mater Interfaces 2022 Jun 23. Epub 2022 Jun 23.

School of Integrated Circuit Science and Engineering, Beihang University, Beijing 100191, China.

Flexible polarization control of the terahertz wave in the wide bandwidth is crucial for numerous applications, such as terahertz communication, material characterization, imaging, and biosensing diagnosis. However, this promise is impeded by the operating bandwidth of circular polarization states, control modes, and the efficiency of the regulation. Here, we report a spintronic terahertz emitter integrated with phase complementary elements, consisting of a liquid crystal and metasurface, to achieve broadband polarization control with high flexibility. This strategy allows the broadband conversion between linear, elliptical, and circular polarization by changing the rotation angle to modulate the space-variant Pancharatnam-Berry phase. The device is characterized with a terahertz time-domain spectroscopy system, demonstrating that the ellipticity of the circular polarization state could keep greater than 0.9 in 0.60-0.99 THz. In the case of an external electro-magnetic field, further polarization modulation experiments are carried out to provide multiple conversion approaches for multi-azimuth. We first propose a method of full broadband polarization state control of the terahertz emitter based on Pancharatnam-Berry phase modulation and an external electro-magnetic field. We believe that such integrated devices with broadband working bandwidth and multiple control modes will make valuable contributions to the development and multi-scene applications of ultrafast terahertz technologies.
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http://dx.doi.org/10.1021/acsami.2c04782DOI Listing
June 2022

Long-term and efficient inhibition of hepatitis B virus replication by AAV8-delivered artificial microRNAs.

Antiviral Res 2022 Jun 19;204:105366. Epub 2022 Jun 19.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. Electronic address:

Chronic hepatitis B virus (HBV) infection remains a global health problem and current treatments are insufficient due to immune tolerance to hepatitis B surface antigen (HBsAg). RNA interference (RNAi) is a more promising approach for antiviral therapy. Here, 17 single artificial microRNAs (amiRNAs) targeting the highly conserved regions of HBV genome were screened to inhibit HBV replication. In addition, we compared three tandem amiRNAs, each containing 3 different amiRNAs, out of which amiRNA135 was selected to be studied in detail. In vitro data showed that amiRNA135 significantly inhibited the replication of different HBV genotypes (including resistant and mutant). In vivo study was carried out by adeno-associated virus 8-mediated gene delivery, we found that the anti-HBV effects of AAV8-amiRNA135 were time and dose-dependent. Serum HBsAg and HBeAg in high dose groups were significantly reduced at 7 days after a single intravenous vector injection, and maintained at low levels throughout a 15-month experiment. Immunohistochemical staining and HBV core particle DNA analysis confirmed that HBV replication in the liver was strongly inhibited by AAV8-amiRNA135. Taken together, our data suggest that AAV8-mediated trimeric amiRNA expression is a promising therapeutic approach for chronic HBV infection.
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http://dx.doi.org/10.1016/j.antiviral.2022.105366DOI Listing
June 2022

The combination of mean and maximum Hounsfield Unit allows more accurate prediction of uric acid stones.

Urolithiasis 2022 Jun 6. Epub 2022 Jun 6.

The First Affiliated Hospital, Urology Department, Hengyang Medical School, University of South China, No. 69, chuanshan Road, Shigu District, Hengyang, 421001, Hunan Province, China.

Based on mean Hounsfield Unit (HuMean), we aimed to evaluate the additional use of standard deviation of Hounsfield Unit (HuStd), minimum Hounsfield Unit (HuMin), and maximum Hounsfield Unit (HuMax) in noncontrast computed tomography (NCCT) to evaluate uric acid (UA) stones more accurately. The data of patients who underwent the NCCT examination and infrared spectroscopy in our hospital from August 2017 to December 2021 were analyzed retrospectively. Based on CT scans, the HuMean, HuStd, HuMin, and HuMax of all patients were measured. The patients were divided into groups according to the stone composition. The attenuation value of mixed stones was in the middle of their pure stones. Except for Str, statistically significant differences between UA stones and other pure stones were observed for HuMean, HuStd, HuMin, and HuMax. A moderate correlation was found between HuMean, HuStd, HuMin, and HuMax and UA stones (r showed -0.585, -0.409, -0.492, and -0.577, respectively). Receiver operator characteristic (ROC) curve showed that the area under the curve (AUC) of HuMean and HuMax were higher than those of HuStd and HuMin (AUC = 0.896, AUC = 0.891 vs. AUC = 0.777, AUC = 0.833). Higher AUC (0.904), specificity (0.899) and positive predictive value (PPV) (0.712) can be obtained by combining HuMean and HuMax in the diagnosis of UA stones. In conclusion, HuMean and HuMax can better predict UA stones than HuStd and HuMin. The combined use of HuMean and HuMax can lead to higher accuracy.
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http://dx.doi.org/10.1007/s00240-022-01333-2DOI Listing
June 2022

Tracking autophagy process with a through bond energy transfer-based ratiometric two-photon viscosity probe.

Biosens Bioelectron 2022 Jun 15;213:114484. Epub 2022 Jun 15.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. Electronic address:

Autophagy is a self-degradation process in cells, which is of vital significance to the health and operation of organisms. Due to the increase of lysosomal viscosity during autophagy, viscosity probes that specifically accumulate in lysosome are powerful tools for monitoring autophagy and investigating related diseases. However, there is still a lack of viscosity-sensitive ratiometric autophagy probes, which restricts the tracking of autophagy with high accuracy in complex physiological environment. Herein, a viscosity-responsive, lysosome targeted two-photon fluorescent probe Lyso-Vis was designed based on through bond energy transfer (TBET) mechanism. The TBET-based probe achieved the separation of two emission baselines, which greatly improved the resolution and reliability of sensing and imaging. Under 810 nm two-photon excitation, the emission intensity ratio of the red and green channel increased with a viscosity dependent manner. Lyso-Vis not only for the first time realized ratiometric sensing of lysosomal viscosity during autophagy process, but also visualized the association of autophagy with inflammation and stroke, and it was applied to explore the activation and inhibition of autophagy during stroke in mice.
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http://dx.doi.org/10.1016/j.bios.2022.114484DOI Listing
June 2022

Theoretical Investigation of Switch Effect on the Efficiency and Adaptivity of Molecular Optoelectronic Conversion Devices.

Chem Asian J 2022 Jun 20:e202200463. Epub 2022 Jun 20.

School of Electronic and Information Engineering (Department of Physics), Qilu University of Technology-Shandong Academy of Sciences, Jinan, Shandong, 250353, P. R. China.

Molecular photoswitch can effectively regulate charge separation (CS) and charge recombination (CR) in donor-acceptor (D-A) systems. However, deformation of the donor-switch-acceptor (D-S-A) systems caused by the switch isomerization will destroy the geometrical stability of the battery. Here we take the planar platinum(II) terpyridyl complex of [Pt( Bu tpy)(-C≡C-Ph) ] as the typical D-A model, designed six D-S-A systems using different photoswitches (dimethyldihydropyrene, fulgimide, arylazopyrazole, N-salicylideneaniline, spiropyran, and dithienylethene, denoted as D-S-A 1-6 hereafter). Our investigations show that the D-S-A 1-6 can absorb visible light of 799 nm, 673 nm, 527 nm, 568 nm, 616 nm, and 629 nm, facilitating electrons transfer from the donor and the switch to the acceptor through the Switch-on channel. Then cationic character of the photoswitch can undergo much more rapid isomerization than the neutral form due to the lower energy barrier. The Switch-off isomer breaks the conjugation of the D-S-A system, effectively turning off the CT channel and forming the CS state. Based on the evaluated conjugated backbone twist (CBT) angle, we found that D-S-A 1, 2, 4, 6 exhibit little configurational change and can be good candidates as the organic solar cell. The proposed D-S-A design controlled by the molecular switch may help to develop a solution for solar-harvesting practical applications.
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http://dx.doi.org/10.1002/asia.202200463DOI Listing
June 2022

CCL5-Secreting Virtual Memory CD8+ T Cells Inversely Associate With Viral Reservoir Size in HIV-1-Infected Individuals on Antiretroviral Therapy.

Front Immunol 2022 26;13:897569. Epub 2022 May 26.

Medical School of Chinese People's Liberation Army (PLA), Beijing, China.

Recent studies highlighted that CD8+ T cells are necessary for restraining reservoir in HIV-1-infected individuals who undergo antiretroviral therapy (ART), whereas the underlying cellular and molecular mechanisms remain largely unknown. Here, we enrolled 60 virologically suppressed HIV-1-infected individuals, to assess the correlations of the effector molecules and phenotypic subsets of CD8+ T cells with HIV-1 DNA and cell-associated unspliced RNA (CA usRNA). We found that the levels of HIV-1 DNA and usRNA correlated positively with the percentage of CCL4+CCL5- CD8+ central memory cells (T) while negatively with CCL4-CCL5+ CD8+ terminally differentiated effector memory cells (T). Moreover, a virtual memory CD8+ T cell (T) subset was enriched in CCL4-CCL5+ T cells and phenotypically distinctive from CCL4+ T subset, supported by single-cell RNA-Seq data. Specifically, T cells showed superior cytotoxicity potentially driven by T-bet and RUNX3, while CCL4+ T subset displayed a suppressive phenotype dominated by JUNB and CREM. In viral inhibition assays, T cells inhibited HIV-1 reactivation more effectively than non-T CD8+ T cells, which was dependent on CCL5 secretion. Our study highlights CCL5-secreting T cells subset as a potential determinant of HIV-1 reservoir size. This might be helpful to design CD8+ T cell-based therapeutic strategies for cure of the disease.
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http://dx.doi.org/10.3389/fimmu.2022.897569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204588PMC
June 2022

miR-212-5p Suppresses Glioma Development via Targeting SUMO2.

Biochem Genet 2022 Jun 18. Epub 2022 Jun 18.

Suqian Hospital Affiliated to Xuzhou Medical University, 223800, Suqian, China.

Recently, increasing studies have suggested that miRNAs play a significant role in the occurrence and development of glioma. More researches are needed to explore the role of miRNAs in glioma, which will help to find new therapeutic targets. miR-212-5p has been reported to be involved in the progression in many cancers. However, whether miR-212-5p has a regulative effect on glioma remains un clear. In this study, we aimed to explore the effect of miR-212-5p on glioma development and its mechanism. Here, we demonstrated that miR-212-5p was lowly expressed in glioma cell. miR-212-5p suppressed the glioma cell proliferation, inhibited the migratory and invasive capabilities and promoted apoptosis in glioma cells. Besides, miR-212-5p also inhibited tumor growth in vivo. We found small ubiquitin-like modifier 2 (SUMO2) was the target of miR-212-5p, and miR-212-5p suppressed SUMO2 expression to regulate the proliferation, migration, and apoptosis of glioma cells. These findings indicated that miR-212-5p may be a possible therapeutic target for the treatment for glioma.
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http://dx.doi.org/10.1007/s10528-022-10236-1DOI Listing
June 2022

Tracer-based characterization of fine carbonaceous aerosol in Beijing during a strict emission control period.

Sci Total Environ 2022 Jun 13;841:156638. Epub 2022 Jun 13.

Institute of Surface-Earth System Science, School of Earth System Science, Tianjin University, Tianjin 300072, China. Electronic address:

Strict emission controls were implemented in Beijing and the surrounding regions in the North China Plain to guarantee good air quality during the 2014 Asia-Pacific Economic Cooperation (APEC) summit. Thus, the APEC period provides a good opportunity to study the sources and formation processes of atmospheric organic aerosol. Here, fine particles (PM, particulate matter with a diameter of 2.5 μm or less) collected in urban Beijing before and during the APEC period were analyzed for molecular tracers of primary and secondary organic aerosol (SOA). The primary organic carbon (POC) and secondary organic carbon (SOC) were also reconstructed using a tracer-based method. The concentrations of biogenic SOA tracers ranged from 1.09 to 34.5 ng m (mean 10.3 ± 8.51 ng m). Monoterpene oxidation products were the largest contributor to biogenic SOA, followed by isoprene- and sesquiterpene-derived SOA. The concentrations of biogenic SOA tracers decreased by 50 % during the APEC, which was largely attributed to the implementation of emission controls by the Chinese government. The increasing mass fractions of biogenic SOA tracers from isoprene and sesquiterpene during the pollution episodes implied that their photooxidation processes contributed to the poor air quality in urban Beijing. The reconstructed biogenic and anthropogenic SOC and POC concentrations were 89.6 ± 96.8 ng m, 570 ± 611 ng m, and 2.49 ± 2.08 μg m, respectively, accounting for 21.9 ± 11.4 % of OC in total. Biomass-burning derived OC was the largest contributor to carbonaceous aerosol over the North China Plain. By comparing the results before and during the APEC, the emission controls effectively mitigated about 34 % of the estimated OC and were more effective at reducing SOC than POC. This suggests that the reduction of the primary organic aerosol loading is harder than SOA over the North China Plain.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156638DOI Listing
June 2022

Plastid development of albino viviparous propagules in woody mangrove Kandelia obovata.

Tree Physiol 2022 Jun 16. Epub 2022 Jun 16.

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China 361102.

The process of plastids developing into chloroplasts is critical for plants to survive. However, such a process in woody plants is less understood. Kandelia obovata is a viviparous mangrove species, the seeds of which germinate on the maternal tree and the hypocotyls continue to develop into mature propagules. Through field observation, we identified rare albino propagules among normal green and brown ones. Towards unveiling propagule plastid development mechanism, albino propagule leaves only have etioplasts, low photosynthesis rates, and drastically reduced chlorophyll a/b and carotenoid contents, but with increased superoxide dismutase activities. To identify candidate genes controlling propagule plastid development, a genome-wide association study (GWAS) was performed between the albino and green propagules. Twenty-five significant single nucleotide polymorphisms were associated with albino propagule plastid development, the most significant SNPs were located on chromosome 1 and 5. Combining transcriptome and GWAS data, significant differentially expressed genes (DEGs) were identified in porphyrin and chlorophyll metabolisms, carotenoid and flavonoid biosynthesis. In particular, KoDELLAs, encoding a transcription factor, and KoCHS, encoding chalcone synthase, may be essential to regulate the albino propagules plastid development through weaken chlorophyll and flavonoid biosynthesis pathways while promoting chlorophyll degradation. Our results provide insights into genetic mechanisms regulating propagule plastid development in woody plants.
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http://dx.doi.org/10.1093/treephys/tpac063DOI Listing
June 2022

Impact of magnetic field on the parallel resistivity.

Phys Rev E 2022 May;105(5-2):055204

Beijing National Laboratory for Condensed Matter Physics and Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

The impact of magnetic field (MF) on the parallel resistivity η_{∥} is studied for strongly magnetized plasmas with the electron thermal gyroradius ρ_{the} smaller than the Debye length λ_{D} but much larger than the Landau length λ_{L}. Two previous papers [P. Ghendrih et al., Phys. Lett. A 119, 354 (1987)10.1016/0375-9601(87)90614-1; S. D. Baalrud and T. Lafleur, Phys. Plasmas 28, 102107 (2021)10.1063/5.0054113] found η_{∥} to increase monotonically with MF. Unfortunately, both works used predetermined electron distribution functions and are thus not self-consistent. In this paper, we analyze the MF dependence of η_{∥} self-consistently by solving the electron magnetized kinetic equation in a Lorentz gaslike approximation. It is found η_{∥} decreases monotonically with MF, with λ_{D} in the usual Coulomb logarithm lnΛ=ln(λ_{D}/λ_{L}) being replaced by ρ_{the}. The underlying physics is that the electrons affected only by the collisions with impact parameters between λ_{L} and ρ_{the} carry almost all the parallel current.
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http://dx.doi.org/10.1103/PhysRevE.105.055204DOI Listing
May 2022

Cobalt(II)-Catalyzed C-H and N-H Functionalization of 1-Arylpyrazolidinones with Dioxazolones as Bifunctional Synthons.

Org Lett 2022 Jun 15. Epub 2022 Jun 15.

Henan Key Laboratory of Chemical Biology and Organic Chemistry, Key Laboratory of Applied Chemistry of Henan Universities, Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou 450052, P. R. China.

Dioxazolone has been attractive as an important synthon for a direct C-H amidation through a nitrene intermediate or Curtius rearrangement to form the isocyanate. However, the combination of two reaction models of dioxazolone has not been reported. Herein, a cobalt-catalyzed C-H and N-H functionalization of 1-arylpyrazolidinones with dioxazolones was developed. The dioxazolones acted as an amidated and carboxamidated reagent. Three C-N bonds were formed in a "one-pot" manner, which promoted the requirement of synthetic diversity.
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http://dx.doi.org/10.1021/acs.orglett.2c01780DOI Listing
June 2022

Estimating population norms for the health-related quality of life of adults in southern Jiangsu Province, China.

Sci Rep 2022 Jun 14;12(1):9906. Epub 2022 Jun 14.

Department of Epidemiology and Biostatistics, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou, 215123, People's Republic of China.

Although national health-related quality of life population norms had been published based on the EuroQol 5-Dimensions 5-levels scale, China is a vast country with diverse cultural and social development in various regions. Therefore, regional population norms may better reflect the health status of residents in a given area. The purpose of the study was to derive the HRQoL population norm for adult general population in southern Jiangsu Province using the EQ-5D-5L scale and explore potential influencing factors. The data were based on a cross-sectional survey conducted in Liyang City from March 2019 to July 2020. EQ-5D-5L utility scores based on Chinese value set and EQ-VAS scores were used to assess HRQoL. The Tobit regression model and generalized linear model were performed to identify the association among potential covariates and HRQoL. The means (95% confidence interval) of the EQ-5D-5L utility scores and EQ-VAS scores were 0.981(0.980-0.983) and 83.6(83.2-83.9), respectively. Younger people (≤ 40 years old) were more likely to experience problems with anxiety or depression. Additionally, women had lower HRQoL scores although multivariate analysis found no statistical difference between the sexes. Lower HRQoL was associated with advanced age, lower socioeconomic status, no spouse, lack of regular physical activities, smoking cessation, and chronic non-communicable diseases. Subjects who declared that they were afflicted by diseases presented significantly lower utility scores, ranging from 0.823 (0.766-0.880) for memory-related diseases to 0.978 (0.967-0.989) for hepatic diseases. Regional population norms of HRQoL are needed in the health economic study owing to the great socioeconomic differences across regions in China. The present study provides HRQoL population norms for adults in southern Jiangsu. These norm values could help policy makers better allocate limited health resources and prioritize service plans.
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http://dx.doi.org/10.1038/s41598-022-13910-xDOI Listing
June 2022

Genetic tracing reveals transcription factor Foxp3-dependent and Foxp3-independent functionality of peripherally induced Treg cells.

Immunity 2022 Jun 7. Epub 2022 Jun 7.

Howard Hughes Medical Institute and Immunology Program, Sloan Kettering Institute, and Ludwig Center at Memorial Sloan Kettering Cancer Center, New York, NY, USA. Electronic address:

Regulatory T (Treg) cells expressing the transcription factor Foxp3 are an essential suppressive T cell lineage of dual origin: Foxp3 induction in thymocytes and mature CD4 T cells gives rise to thymic (tTreg) and peripheral (pTreg) Treg cells, respectively. While tTreg cells suppress autoimmunity, pTreg cells enforce tolerance to food and commensal microbiota. However, the role of Foxp3 in pTreg cells and the mechanisms supporting their differentiation remain poorly understood. Here, we used genetic tracing to identify microbiota-induced pTreg cells and found that many of their distinguishing features were Foxp3 independent. Lineage-committed, microbiota-dependent pTreg-like cells persisted in the colon in the absence of Foxp3. While Foxp3 was critical for the suppression of a Th17 cell program, colitis, and mastocytosis, pTreg cells suppressed colonic effector T cell expansion in a Foxp3-independent manner. Thus, Foxp3 and the tolerogenic signals that precede and promote its expression independently confer distinct facets of pTreg functionality.
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http://dx.doi.org/10.1016/j.immuni.2022.05.010DOI Listing
June 2022

p38 MAPK Is a Major Regulator of Amyloid Beta-Induced IL-6 Expression in Human Microglia.

Mol Neurobiol 2022 Jun 13. Epub 2022 Jun 13.

Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, MSC908, Charleston, SC, 29425, USA.

The accumulation of amyloid beta (Aβ) plaques in the brain is a hallmark of Alzheimer's disease (AD) pathology. Microglial activation-mediated neuroinflammation has been implicated in the pathogenesis of AD and the expression levels of interleukin-6 (IL-6) were increased in the brains of AD patients. However, the mechanisms by which IL-6 expression is regulated in human microglia are incompletely understood. Here, we show that Aβ oligomers (Aβ) dose-dependently stimulate IL-6 expression in HMC3 human microglial cells. Treatment with Aβ promotes the transcription of IL-6 and tumor necrosis factor α (TNFα) mRNAs in both HMC3 and THP-1 cells. Mechanistic studies reveal that Aβ-induced increase of IL-6 secretion is associated with the activation of p38 mitogen-activated protein kinase (p38 MAPK). Inhibition of p38 MAPK by BIRB 796 or SB202190 abrogates Aβ-induced increase of IL-6 production. Through analyzing brain specimens, we found that the immunoreactivity for IL-6 and phosphorylated (the activated form) p38 MAPK was markedly higher in microglia of AD patients than in age-matched control subjects. Moreover, our studies identified the co-localization of IL-6 with phosphorylated p38 MAPK in microglia in the cortices of AD patients. Taken together, these results indicate that p38 MAPK is a major regulator of Aβ-induced IL-6 production in human microglia, which suggests that targeting p38 MAPK may represent a new approach to ameliorate Aβ accumulation-induced neuroinflammation in AD.
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http://dx.doi.org/10.1007/s12035-022-02909-0DOI Listing
June 2022

TNF-α/Anti-TNF-α drugs and its effect on pregnancy outcomes.

Expert Rev Mol Med 2022 Jun 10:1-18. Epub 2022 Jun 10.

Department of Obstetrics and Gynaecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.

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http://dx.doi.org/10.1017/erm.2022.18DOI Listing
June 2022

Electrolytes with Micelle-Assisted Formation of Directional Ion Transport Channels for Aqueous Rechargeable Batteries with Impressive Performance.

Nanomaterials (Basel) 2022 Jun 4;12(11). Epub 2022 Jun 4.

Engineering & Technology Center of Electrochemistry, School of Chemistry and Chemical Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

Low-cost and ecofriendly electrolytes with suppressed water reactivity and raised ionic conductivity are desirable for aqueous rechargeable batteries because it is a dilemma to decrease the water reactivity and increase the ionic conductivity at the same time. In this paper, LiSO-NaSO-sodium dodecyl sulfate (LN-SDS)-based aqueous electrolytes are designed, where: (i) Na ions dissociated from SDS increase the charge carrier concentration, (ii) DS/SO anions and Li/Na cations are capable of trapping water molecules through hydrogen bonding and/or hydration, resulting in a lowered melting point, (iii) Li ions reduce the Krafft temperature of LN-SDS, (iv) Na and SO ions increase the low-temperature electrolyte ionic conductivity, and (v) SDS micelle clusters are orderly aggregated to form directional ion transport channels, enabling the formation of quasi-continuous ion flows without (r.t.) and with (≤0 °C) applying voltage. The screened LN-SDS is featured with suppressed water reactivity and high ionic conductivity at temperatures ranging from room temperature to -15 °C. Additionally, NaTi(PO)‖LiMnO batteries operating with LN-SDS manifest impressive electrochemical performance at both room temperature and -15 °C, especially the cycling stability and low-temperature performance.
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http://dx.doi.org/10.3390/nano12111920DOI Listing
June 2022

Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic .

Int J Mol Sci 2022 May 24;23(11). Epub 2022 May 24.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Germline stem cells (GSCs) are a group of unique adult stem cells in gonads that act as important transmitters for genetic information. Donor GSCs have been used to produce offspring by transplantation in fisheries. In this study, we successfully isolated and enriched GSCs from the ovary, ovotestis, and testis of , one of the most important breeding freshwater fishes in China. Transcriptome comparison assay suggests that a distinct molecular signature exists in each type of GSC, and that different signaling activities are required for the maintenance of distinct GSCs. Functional analysis shows that fGSCs can successfully colonize and contribute to the germline cell lineage of a host zebrafish gonad after transplantation. Finally, we describe a simple feeder-free method for the isolation and enrichment of GSCs that can contribute to the germline cell lineage of zebrafish embryos and generate the germline chimeras after transplantation.
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http://dx.doi.org/10.3390/ijms23115861DOI Listing
May 2022

A fast responsive and cell membrane-targetable near-infrared HS fluorescent probe for drug resistance bioassays in chemotherapy.

Chem Commun (Camb) 2022 Jun 28;58(52):7301-7304. Epub 2022 Jun 28.

Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, South-Central Minzu University, Wuhan 430074, China.

A cell membrane-targeted near-infrared fluorescent probe, CMCu-HS, was fabricated through employing hydrophobic chains and cyclen-Cu as targeting and recognition groups, respectively. NIR fluorescence of CMCu-HS can significantly increase after reacting with HS by removing the quenchable Cu. This probe exhibited high selectivity and an extremely fast response rate. Cell imaging results demonstrated that there was a close relationship between the overexpression of NFS1 and drug resistance and inhibition of NFS1 was beneficial for improving the efficacy of chemotherapy.
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http://dx.doi.org/10.1039/d2cc02430fDOI Listing
June 2022

Spin-Decoupled Transflective Spatial Light Modulations Enabled by a Piecewise-Twisted Anisotropic Monolayer.

Adv Sci (Weinh) 2022 Jun 4:e2202424. Epub 2022 Jun 4.

National Laboratory of Solid State Microstructures, Key Laboratory of Intelligent Optical Sensing and Manipulation, and College of Engineering and Applied Sciences, Nanjing University, Nanjing, 210023, China.

Wavefront control lies at the heart of modern optics. Metasurfaces with specifically tailored resonators can encode different phases to two orthogonal polarization components, but suffer from wavelength-dependent efficiency, sophisticated fabrication, and limited size. Liquid crystals, another excellent candidate for planar optics, are restricted to spin-coupled conjugated phase modulations. Planar optics with spin-decoupled functions is expected to release the multifunctionality of modern optics. Here, a spin-decoupled transflective spatial light modulator is presented with a piecewise-twisted anisotropic monolayer. The phases of reflected and transmitted light can be independently customized by preprogramming the initial orientations of the periodic helix and mirror-symmetric dual-twist configuration, respectively. A transflective orbital angular momentum encoder and decoder is demonstrated, which is simultaneously compatible with different multiplexing techniques. This work releases the multifunctionality of advanced planar optics and may upgrade existing devices in optical informatics.
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http://dx.doi.org/10.1002/advs.202202424DOI Listing
June 2022

Detection & analysis of inflammatory cytokines in tears of patients with lacrimal duct obstruction.

Indian J Med Res 2021 06;154(6):888-894

Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, P.R. China.

Background & Objectives: Tear proteomic changes can be a candidate etiopathogenesis of lacrimal duct obstruction diseases (LDODs). Studies on proteomics have focused primarily on nasolacrimal duct obstruction, and some specific inflammatory cytokines such as interferon (IFN)-α2a, interleukin (IL)-8 and IL-10, have not been investigated. In addition, differences in inflammatory cytokines in tears according to the LDOD subtype have not been reported. This study aimed to quantitatively compare inflammatory cytokines in tears from patients with LDOD and investigate tear-cytokine differences among different LDOD subtypes.

Methods: Tear samples were collected from both eyes of 30 patients with unilateral LDOD: five patients with prelacrimal obstruction, five with acute dacryocystitis and 20 with chronic dacryocystitis. The contralateral eyes were used as controls. IFN-α2a, IFN-β, IFN-γ, IL-17A, IL-6, IL-8, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF)-A, induced protein-10 (IP-10) and monocyte chemotactic protein-1 (MCP-1) were quantified in all samples.

Results: The expression of eight cytokines (except for IP-10 and MCP-1) were significantly increased in the affected eyes compared with those in the control eyes. The levels of nine inflammatory cytokines (except for IP-10) in the affected eyes of patients with chronic dacryocystitis were higher than those in the affected eyes of patients with prelacrimal obstruction. In addition, patients with chronic dacryocystitis presented significantly higher IFN-γ level than those with prelacrimal obstruction or acute dacryocystitis.

Interpretation & Conclusions: Specific pro-inflammatory cytokines were increased in tears of patients with LDOD compared with those in the controls. The specific cytokine profiles observed in the tears of individuals with different LDOD subtypes may be associated with the unique aetiopathogenesis of these conditions.
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http://dx.doi.org/10.4103/ijmr.IJMR_1435_19DOI Listing
June 2021

Endovascular Treatment Versus Best Medical Management in Acute Basilar Artery Occlusion Strokes: Results From the ATTENTION Multicenter Registry.

Circulation 2022 Jun 3:101161CIRCULATIONAHA121058544. Epub 2022 Jun 3.

Department of Neurology, Anqing Hospital Affiliated to Anhui Medical University, China (X.S.).

Background: The authors compare the effectiveness and safety of endovascular treatment (EVT) versus best medical management (BMM) in strokes attributable to acute basilar artery occlusion (BAO).

Methods: The present analysis was based on the ongoing, prospective, multicenter ATTENTION (Endovascular Treatment for Acute Basilar Artery Occlusion) trial registry in China. Our analytic sample comprised 2134 patients recruited at 48 sites between 2017 and 2021 and included 462 patients who received BMM and 1672 patients who received EVT. We performed an inversed probability of treatment weighting analysis. Qualifying patients had to present within 24 hours of estimated BAO. The primary clinical outcome was favorable functional outcome (modified Rankin Scale score, 0-3) at 90 days. We also performed a sensitivity analysis with the propensity score matching-based and the instrumental variable-based analysis.

Results: In our primary analysis using the inversed probability of treatment weighting-based analysis, there was a significantly higher rate of favorable outcome at 90 days among EVT patients compared with BMM-treated patients (adjusted relative risk, 1.42 [95% CI, 1.19-1.65]; absolute risk difference, 11.8% [95% CI, 6.9-16.7%]). The mortality was significantly lower (adjusted relative risk, 0.78 [95% CI, 0.69-0.88]; absolute risk difference, -10.3% [95% CI, -15.8 to -4.9]) in patients undergoing EVT. Results were generally consistent across the secondary end points. Similar associations were seen in the propensity score matching-based and instrumental variable-based analysis.

Conclusion: In this real-world study, EVT was associated with significantly better functional outcomes and survival at 90 days. Well-designed randomized studies comparing EVT with BMM in the acute BAO are needed.

Registration: URL: www.chictr.org.cn Unique identifier: ChiCTR2000041117.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.121.058544DOI Listing
June 2022
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