Publications by authors named "Wei He"

2,646 Publications

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[Chloroquine Enhances BIIB021-induced Apoptosis in Chronic Myeloid Leukemia Cells Bearing T315I Mutation].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2022 Aug;30(4):1005-1010

Department of Hematology, The Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua 321000, Zhejiang Province, China.E-mail:

Objective: To explore the combined pro-apoptosis effect of HSP90 inhibitor BIIB021 and chloroquine (CQ) in chronic myeloid leukemia (CML) cells bearing T315I mutation and its mechanism.

Methods: The p210-T315I cells were divided into 4 groups by different treatment: control, BIIB021, CQ, and BIIB021 + CQ. After treated with BIIB021 or/and CQ for 24 hours, Annexin V/PI binding assay was used to detect apoptosis rates of CML cells. DAPI staining was used to observe nuclear fragmentation, and Western blot was used to detect the expression of caspase 3, PARP (apoptosis related proteins) and p62, LC3-I/II (autophagy related proteins). P210-T315I cells were inoculated subcutaneously into mice and CML mouse models were established. The mice in treatment groups were injected with BIIB021 and/or CQ while mice in control group were treated with PBS and normal saline. The tumor volume of mice was measured every 4 days, and protein level of cleaved-caspase 3 and LC3-II in tumor tissue were detected by immunohistochemistry.

Results: The results showed that BIIB021 induced apoptosis of CML cells in a dose-dependent manner ( r=0.91). CQ could enhance the apoptosis-inducing effect of BIIB021. Flow cytometry analysis results showed that the apoptosis rate of p210-T315I cells in combination group was higher than that in BIIB021 or CQ only group (P<0.05). DAPI staining showed nuclear fragmentation in combination group could be observed more obviously. Western blot analysis showed that BIIB021 could induce LC3-I to convert to LC3-II and decrease p62 protein levels (P<0.05). Moreover, the combination group had higher expression of LC3-II, p62 (P<0.05), activated PARP and activated caspase 3 than BIIB021 only group (P<0.05). Besides, experiment in vivo showed the mean tumor volume in co-treatment group was lower than that in single drug group (P<0.01). Immunohistochemistry of tumor tissue also showed the protein level of cleaved-caspase 3 and LC3-II in combined group was higher than that in BIIB021 only group.

Conclusion: HSP90 inhibitor BIIB021 induced significant apoptosis of CML cells bearing T315I both in vivo and in vitro. CQ can enhance this effect probably by autophagy inhibition.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.04.005DOI Listing
August 2022

Determinants and effectiveness of extending the duration of adjuvant hormone therapy beyond 5 years in breast cancer patients.

Cancer Res 2022 Aug 18. Epub 2022 Aug 18.

Karolinska Institute, Stockholm, Sweden.

Clinical guidelines have recommended high-risk breast cancer patients to extend adjuvant hormone therapy beyond 5 years. However, the prevalence, predictors, and outcomes of extended adjuvant hormone therapy in the real world remain unknown. By linking six Swedish health registries, we prospectively followed 13168 breast cancer patients (2005-2020) from their first prescription of tamoxifen or aromatase inhibitors, and categorized them as extending or not extending adjuvant hormone therapy. Cox regression analysis was used to investigate whether extended therapy was associated with breast cancer outcomes. Among breast cancer patients who were recommended to extend adjuvant hormone therapy by the national guidelines, the proportion of women who extended therapy increased 5 fold during the past 10 years, reaching 80.9% during 2018-2020. Patients were more likely to extend therapy after completing 5-year adjuvant hormone therapy if they were young (< 40 vs ≥ 65 years: OR, 1.71; 95% CI, 1.13-2.58), had positive lymph nodes (OR, 2.25; 95% CI, 1.85-2.73), had high tumor grade (grade 3 vs 1: OR,1.79; 95% CI, 1.34-2.39), received chemotherapy (OR, 5.22; 95% CI, 4.19-6.50), had first-degree relatives who died from breast cancer (OR, 1.84; 95% CI, 1.21-2.81), or had a high income (OR, 1.23; 95% CI, 1.01-1.49). Extended use of adjuvant hormone therapy was statistically significantly associated with improved disease-free survival (HR, 0.72; 95 CI%, 0.55-0.95). This study provides real-world evidence showing the use and improved breast cancer outcomes of extended adjuvant hormone therapy beyond 5 years.
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http://dx.doi.org/10.1158/0008-5472.CAN-22-0900DOI Listing
August 2022

Highly selective acid-catalyzed olefin isomerization of limonene to terpinolene by kinetic suppression of overreactions in a confined space of porous metal-macrocycle frameworks.

Chem Sci 2022 Aug 3;13(30):8752-8758. Epub 2022 Jun 3.

Department of Chemistry, Graduate School of Science, The University of Tokyo Tokyo 113-0033 Japan

Natural enzymes control the intrinsic reactivity of chemical reactions in the natural environment, giving only the necessary products. In recent years, challenging research on the reactivity control of terpenes with structural diversity using artificial host compounds that mimic such enzymatic reactions has been actively pursued. A typical example is the acid-catalyzed olefin isomerization of (+)-limonene, which generally gives a complex mixture due to over-isomerization to thermodynamically favored isomers. Herein we report a highly controlled conversion of (+)-limonene by kinetic suppression of over-isomerization in a confined space of a porous metal-macrocycle framework (MMF) equipped with a Brønsted acid catalyst. The terminal double bond of (+)-limonene migrated to one neighbor, preferentially producing terpinolene. This reaction selectivity was in stark contrast to the homogeneous acid-catalyzed reaction in bulk solution and to previously reported catalytic reactions. X-ray structural analysis and examination of the reaction with adsorption inhibitors suggest that the reactive substrates may bind non-covalently to specific positions in the confined space of the MMF, thereby inhibiting the over-isomerization reaction. The nanospaces of the MMF with substrate binding ability are expected to enable highly selective synthesis of a variety of terpene compounds.
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http://dx.doi.org/10.1039/d2sc01561gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350587PMC
August 2022

Nasal characteristics in patients with asymmetric mandibular prognathism.

Am J Orthod Dentofacial Orthop 2022 Aug 13. Epub 2022 Aug 13.

Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China; National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, China; National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China. Electronic address:

Introduction: To objectively quantify nasal characteristics of patients with asymmetric mandibular prognathism and to evaluate the association between nasal asymmetry and dentofacial abnormalities.

Methods: Ninety adult patients with asymmetric mandibular prognathism were included. Images were captured during pretreatment using 3-dimensional stereophotogrammetry. A total of 7160 uniformly sampled quasi-landmarks were automatically identified on each facial image to establish correspondence using a template mapping technique. Fifteen commonly used anatomic landmarks were automatically located on each image through barycentric to Cartesian coordinate conversion. Nasal characteristics and asymmetry were quantified by anthropometric linear distances, angular measurements, and surface-based analysis. The degree of the nasal, chin, and periorbital asymmetry in a patient was scored using a root-mean-squared error between the left and right sides. The correlations among these regional asymmetries were evaluated.

Results: The nasal tip was significantly shifted to the deviated side of the chin, and the nostrils were asymmetrical. The location and degree of nasal asymmetry varied among patients with asymmetric mandibular prognathism. The level of nasal asymmetry was significantly and positively correlated with chin and periorbital asymmetry.

Conclusions: Nasal asymmetry is present in asymmetric mandibular prognathism patients. Furthermore, it is positively associated with periorbital deviation and chin deviation. Individualized nasal asymmetry evaluation should be performed, and clinicians should inform patients about preexisting nasal asymmetry.
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http://dx.doi.org/10.1016/j.ajodo.2021.06.023DOI Listing
August 2022

Effect of a Reminder System on Pre-exposure Prophylaxis Adherence in Men Who Have Sex With Men: Prospective Cohort Study Based on WeChat Intervention.

J Med Internet Res 2022 Aug 11;24(8):e37936. Epub 2022 Aug 11.

School of Public Health and Management, Chongqing Medical University, Chongqing, China.

Background: The efficacy of pre-exposure prophylaxis (PrEP) is highly dependent on adherence, and one of the main reasons for poor adherence is forgetfulness. Therefore, it is important to explore how to remind users to take their medicine on time.

Objective: This study aims to explore the effect of a reminder system on PrEP adherence in men who have sex with men (MSM) to improve adherence. The main function of the reminder system based on the WeChat social media app is to send daily messages to PrEP users reminding them to take their medicine.

Methods: An open-label, multicenter, prospective cohort study of PrEP in HIV-negative MSM was conducted from November 2019 to June 2021. Study participants who met the criteria were randomly divided into 2 groups: no-reminder group and reminder group. Both groups received daily oral PrEP with follow-up every 3 months. Adherence was measured on the basis of self-report and was defined as the percentage of medications taken on time. Participants in the reminder group scanned a WeChat QR code and received a reminder message every day. Participants in the no-reminder group took daily oral medicines without reminders. The longitudinal trajectories of adherence for both groups were displayed to compare the variability in adherence at each time point. The association between the changes in adherence (no change, improvement, decline) at each time point and the use of the reminder system was analyzed by multinomial logistic regression models to further explore the effectiveness of the system.

Results: A total of 716 MSM were included in the analysis, that is, 372 MSM in the no-reminder group and 344 MSM in the reminder group. Adherence in the no-reminder group fluctuated between 0.75 and 0.80 and that in the reminder group gradually increased over time from 0.76 to 0.88. Adherence at each time point was not statistically different between the 2 groups. Further analysis showed that an improvement in adherence in the early stage was associated with the use of the reminder system (odds ratio [OR] 1.65, 95% CI 1.01-2.70; P=.04). An improvement in average adherence compared to initial adherence was positively associated with the use of the reminder system (OR 1.82, 95% CI 1.10-3.01; P=.02).

Conclusions: The effect of the reminder system on PrEP adherence in MSM was more significant in the early stage, which is related to the increased motivation of users and the development of medicine-taking habits. The reminder system is potentially effective for early-stage medicine management, encouraging users to develop healthy medicine-taking habits and to increase their adherence.

Trial Registration: Chinese Clinical Trial ChiCTR190026414; http://www.chictr.org.cn/showproj.aspx?proj=35077.
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http://dx.doi.org/10.2196/37936DOI Listing
August 2022

SEPTIN9-SDC2-VIM methylation signature as a biomarker for the early diagnosis of colorectal cancer.

Am J Cancer Res 2022 15;12(7):3128-3140. Epub 2022 Jul 15.

Harbin Medical University Cancer Hospital Colorectal Cancer Center Harbin 150086, Heilongjiang, China.

The accurate detection of colorectal cancer (CRC) at its initial stage can reduce mortality. However, the broad application of endoscopy has been limited due to the invasive procedure and patient noncompliance. Liquid biopsy with subsequent mapping of methylation in specific cell-free DNA (cfDNA) may represent an alternative approach for early diagnosis. In this study, we have developed a minimal-invasive blood-based test for detection of precancerous lesions and early-stage CRC. Using TCGA M450K methylation data, we identified candidate methylation sites with the highest Fold Change (FC) for three genes (SEPTIN9, SDC2 and VIM), which were selected from previous studies. Based on logistic regression models, we developed a 3-gene methylation signature for CRC diagnosis with high accuracy (Sensitivity =0.959, Specificity =1, AUC =0.997). Using independent public databases and data from blood samples, this model has demonstrated superior performance. The AUC was 0.919-1 and 0.905-0.916 in public tissue database for CRC and blood sample data, respectively. Thus, our proposed 3-gene methylation signature has a more reliable performance than other methods. Furthermore, signal enhancement effect of 3-gene methylation signature can improve the accuracy of early diagnosis for CRC, which demonstrates the potential for clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360219PMC
July 2022

Kyphoplasty for thoracic and lumbar fractures with an intravertebral vacuum phenomenon in ankylosing spondylitis patients.

Front Surg 2022 29;9:962723. Epub 2022 Jul 29.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Intravertebral vacuum phenomenon (IVP) is a special sign after vertebral fractures, which is common in patients with ankylosing spondylitis (AS) and may indicate pseudarthrosis and bone nonunion that lead to spinal instability. The objective of this study is to evaluate the efficacy and safety of kyphoplasty (KP) in treating such types of vertebral fractures with AS.

Methods: Sixteen patients with AS suffering from thoracic or lumbar fractures with IVP received KP from 2015 to 2020 and were monitored for more than 1 year. The visual analog scale (VAS) score was used to evaluate back pain relief. The Oswestry Disability Index (ODI) questionnaire was used to assess the improvement of the patients' living quality. The anterior and middle vertebral height restoration ratio (AVH, MVH) and the kyphotic angle (KA) were used to evaluate the radiographic results.

Results: The mean follow-up period was 20.8 months (12-28 months). The VAS and ODI significantly reduced at 3 days, 3 months after surgery, and at the last follow-up compared with the preoperative outcomes (< 0.05). The AVH and MVH were significantly increased compared with the preoperative outcomes (< 0.05). There was a significant correction in the KA between pre- and postoperative assessments (< 0.05). Asymptomatic intradiscal polymethylmethacrylate (PMMA) cement leakage was found in two patients.

Conclusions: For thoracic or lumbar fractures with IVP in AS patients, KP may be safe and effective, which achieves pain relief and satisfying functional improvement, restores the anterior and middle height, and corrects the kyphotic angle of the fractured vertebra.
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http://dx.doi.org/10.3389/fsurg.2022.962723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372763PMC
July 2022

Expedient Synthesis of Ubiquitin-like Protein ISG15 Tools Through Chemo-Enzymatic Ligation Catalyzed by a Viral Protease Lbpro.

Angew Chem Int Ed Engl 2022 Aug 12. Epub 2022 Aug 12.

Tsinghua University, Department of Chemistry, CHINA.

Ubiquitin (Ub)-like protein ISG15 (interferon-stimulated gene 15) regulates innate immunity and is implicated in the evasion of the host response by viruses such as SARS-CoV-2. Dissecting ISGylation pathways recently received increasing attention which can illuminate the biological functions of ISG15 and inform related disease interventions, but such studies necessitate the expedient preparation and development of various ISG15 protein tools. Here, we report a finding that the leader protease (Lb pro ) encoded by foot-and-mouth disease virus can promote ligation reactions between recombinant ISG15 and synthetic glycyl compounds, enabling efficient preparation of various ISG15 protein tools such as ISG15-propargylamide and ISG15-rhodamine110, which are needed for cellular proteomic studies of deISGylases, and the screening and evaluation of inhibitors against SARS-CoV-2 papain-like protease (PLpro). Furthermore, the Lb pro ligation strategy can also be used for loading recombinant ISG15 onto the lysine of a synthetic peptide through an isopeptide bond, and for chemo-enzymatic preparation of Ub and NEDD8 protein tools.
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http://dx.doi.org/10.1002/anie.202206205DOI Listing
August 2022

Tapped-inductor bi-directional Cuk converter with high step-up/down conversion ratio and its optimum design.

Sci Rep 2022 Aug 12;12(1):13745. Epub 2022 Aug 12.

Fujian Engineering Research Center of Safety Control for Ship Intelligent Navigation, College of Physics and Electronic Information Engineering, Minjiang University, Fuzhou, Fujian, China.

A bidirectional DC-DC converter is required for an energy storage system. High efficiency and a high step-up and step-down conversion ratio are the development trends. In this research, a series of bidirectional high-gain Cuk circuits was derived by combining tapped inductors and bidirectional Cuk. After analyzing and comparing the characteristics of each circuit, a bidirectional high-gain Cuk circuit with a tapped-inductor (reverse coupling) was proposed. The proposed converter has a simple structure and a high voltage gain in both the step-down (Buck) and step-up (Boost) operation modes. The voltage stress of S was low. The voltage stress of S was high, however, and this is a disadvantage of the proposed converter. The proposed circuit's characteristics were thoroughly examined, including the voltage gain characteristics and the design of the main parameters. We established a power loss model of the new topology, and the tapped-inductor turn ratio was optimized for high efficiency. Finally, a 400 W experimental implementation of the converter was shown to achieve efficiencies of 93.5% and 92.4% in the step-up and step-down modes, respectively. These findings verified the validity of the proposed circuit's theoretical analysis.
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http://dx.doi.org/10.1038/s41598-022-17801-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374707PMC
August 2022

Pitfalls in the diagnosis and treatment of fat-poor angiomyolipoma of the renal pelvis mimicking urothelial carcinoma: report of three rare cases.

Am J Transl Res 2022 15;14(7):4848-4854. Epub 2022 Jul 15.

Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan, Shandong, China.

Angiomyolipoma (AML) represents the most frequent benign neoplasm of the kidney. It arises mostly in the cortex and protrudes into the perirenal space. It is extremely rare for a fat-poor AML to originate from the renal sinus, invade the pelvis, and present with hematuria. Because of the rarity of this lesion, differentiating it from a urothelial carcinoma is difficult, thereby making a preoperative diagnosis and management complex and challenging. We report three cases of fat-poor AML centered within the renal pelvis mimicking a urothelial carcinoma that underwent radical nephroureterectomy. The clinical characteristics, surgical management, and prognosis are discussed to achieve better preoperative evaluation of these entities. This is the first report of fat-poor AMLs involving the renal pelvis and presenting with hematuria. Nephron-sparing treatment is crucial for patients with these entities. Accurate diagnosis may allow partial resection or kidney-preserving treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360889PMC
July 2022

Adapting the WEPP Hillslope Model and the TLS Technology to Predict Unpaved Road Soil Erosion.

Int J Environ Res Public Health 2022 Jul 28;19(15). Epub 2022 Jul 28.

College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.

Unpaved road erosion have been recognized as important sediment sources in a watershed. To evaluate where and when road erosion occurs, the soil loss along road segments should be precisely predicted with process-based erosion models. Methods: The hillslope version of the Water Erosion Prediction Project (WEPP) was used to estimate soil loss from 20 typical road segments in the red soil region of South China. Terrestrial laser scanning (TLS)-measured soil losses were used to validate the model simulations. The results showed that the WEPP model could reasonably predict the total soil loss in relatively short (less than 100 m) and gentle (slope gradient lower than 10%) road segments. In contrast, soil loss would be underestimated for long or steep road segments. Detailed outputs along roads revealed that most of the peak soil loss rates were underestimated. It might due to the linear critical shear stress theory in the WEPP model. Additionally, the lack of upstream flow was found to be connected to the relatively low model efficiency. Nevertheless, the WEPP simulation could accurately fit erosion trend and predict the peak soil loss positions along road segments. Conclusions: The WEPP model could be adopted to evaluate the erosion risk of unpaved roads in the red soil region of South China.
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http://dx.doi.org/10.3390/ijerph19159213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367844PMC
July 2022

Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin.

Analyst 2022 Aug 10. Epub 2022 Aug 10.

Department of Chemistry, School of Pharmacy, The Fourth Military Medical University, 169 Changle West Road, Xi'an, 710032, P. R. China.

In this work, the first endoplasmic reticulum-targeted near-infrared fluorescent probe, ISO-Chy, with a dicyanoisophorone derivative as a fluorophore is reported by introducing the recognition group of 4-bromobutyl for chymotrypsin detection. The probe can be easily synthesized and has shown satisfactory sensitivity and selectivity to chymotrypsin. Meanwhile, ISO-Chy has a large Stokes shift (135 nm) to minimize self-absorption and interference from autofluorescence and then generate significant fluorescence enhancement upon incubation with chymotrypsin. Additionally, ISO-Chy has an excellent ability to target the endoplasmic reticulum, along with preferable Pearson's correlation coefficients () of 0.9411 and 0.9522 in P815 cells and HepG2 cells, respectively. Moreover, ISO-Chy was successfully utilized to visualize endogenous chymotrypsin in P815 cells and HepG2 cells and was first used to detect chymotrypsin activity in HepG2 tumor-bearing mice. These findings indicate that ISO-Chy could be an effective tool for detecting endogenous chymotrypsin activity, supporting its use for investigating chymotrypsin function in pathologic processes.
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http://dx.doi.org/10.1039/d2an01013eDOI Listing
August 2022

DNA methylation analysis for smoking status prediction in the Chinese population based on the methylation-sensitive single-nucleotide primer extension method.

Forensic Sci Int 2022 Jul 29;339:111412. Epub 2022 Jul 29.

Department of Forensic Medicine, School of Basic Medical Sciences, Central South University, No172. Tongzipo Road, Changsha 410013, Hunan, PR China. Electronic address:

In some criminal cases, the identity of suspect is unknown and there is no matching DNA profile in the DNA database. Forensic DNA Phenotyping can provide useful investigative information for these cases. Most forensic studies focus on visible characteristics rather than behavioral characteristics. However, smoking is prevalent in the Chinese population, and DNA methylation is the most promising biomarker for smoking. We collected 204 whole blood samples from the Chinese population and measured methylation levels of 9 smoking-related CpG loci using the methylation-sensitive single-nucleotide primer extension method (Ms-SnuPE). But the single-base extension primers of loci cg12803068 and cg21566642 contained other CpG sites, which may introduce bias, and only the other 7 CpG loci were included in subsequent statistical analysis. The methylation level of locus cg05575921 near the aromatic hydrocarbon receptor repressor (AHRR) gene was much lower in the current smoker group than in the never smoker group. To evaluate the ability of each of 7 CpG loci to predict smoking status, the logistic regression (LR) models were established separately, and locus cg05575921 had the best ability to predict smoking status compared with the other 6 loci. Then, combined (including loci cg19572487, cg05575921, cg23480021, cg23576855, cg21161138, cg01940273, and cg09935388) and stepwise (including loci cg05575921 and cg01940273) multinomial logistic regression (MLR) models were also established. Both combined and stepwise MLR models had good efficiencies in predicting smoking status, and outperformed the above 7 LR models. However, the accuracy, specificity and area under the curve (AUC) of stepwise MLR model in the testing dataset were slightly higher than those of combined MLR model, and the stepwise MLR model required less loci information. Therefore, the stepwise MLR model based on 2 significant CpG loci was more recommended model for predicting smoking status in the Chinese population, and the formula was as follow: P = 1/(1 +e). Mainly 2 CpG loci (cg05575921 and cg01940273) played a major role in the prediction of smoking status, and the other 5 CpG loci contributed less. Moreover, for evaluating the ability of each of 7 CpG loci to predict cigarette consumption, the polynomial regression formulas were established separately. As the adjusted R was between 0.00 and 0.20, the methylation levels of these 7 loci were not closely associated with the cigarette consumption. Our methylation assay is simple, economical, and available in conventional forensic laboratories, and may be useful in assessing the smoking status of unknown suspects.
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http://dx.doi.org/10.1016/j.forsciint.2022.111412DOI Listing
July 2022

Integrated Bioinformatic Analysis of the Shared Molecular Mechanisms Between Osteoporosis and Atherosclerosis.

Front Endocrinol (Lausanne) 2022 22;13:950030. Epub 2022 Jul 22.

The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: Osteoporosis and atherosclerosis are common in the elderly population, conferring a heavy worldwide burden. Evidence links osteoporosis and atherosclerosis but the exact underlying common mechanism of its occurrence is unclear. The purpose of this study is to further explore the molecular mechanism between osteoporosis and atherosclerosis through integrated bioinformatic analysis.

Methods: The microarray data of osteoporosis and atherosclerosis in the Gene Expression Omnibus (GEO) database were downloaded. The Weighted Gene Co-Expression Network Analysis (WGCNA) and differentially expressed genes (DEGs) analysis were used to identify the co-expression genes related to osteoporosis and atherosclerosis. In addition, the common gene targets of osteoporosis and atherosclerosis were analyzed and screened through three public databases (CTD, DISEASES, and GeneCards). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed by Metascape. Then, the common microRNAs (miRNAs) in osteoporosis and atherosclerosis were screened out from the Human microRNA Disease Database (HMDD) and the target genes of whom were predicted through the miRTarbase. Finally, the common miRNAs-genes network was constructed by Cytoscape software.

Results: The results of common genes analysis showed that immune and inflammatory response may be a common feature in the pathophysiology of osteoporosis and atherosclerosis. Six hub genes (namely, COL1A1, IBSP, CTSD, RAC2, MAF, and THBS1) were obtained taking interaction of different analysis results. The miRNAs-genes network showed that has-let-7g might play an important role in the common mechanisms between osteoporosis and atherosclerosis.

Conclusion: This study provides new sights into shared molecular mechanisms between osteoporosis and atherosclerosis. These common pathways and hub genes may offer promising clues for further experimental studies.
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http://dx.doi.org/10.3389/fendo.2022.950030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353191PMC
August 2022

An Individualized Recommendation for Controlled Ovary Stimulation Protocol in Women Who Received the GnRH Agonist Long-Acting Protocol or the GnRH Antagonist Protocol: A Retrospective Cohort Study.

Front Endocrinol (Lausanne) 2022 22;13:899000. Epub 2022 Jul 22.

Department of Obstetrics and Gynecology, Women and Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: The GnRH agonist long-acting protocol and GnRH antagonist protocol are widely used in ovarian stimulation. Which protocol eliciting higher live birth rate for IVF/ICSI patients with different ages, different ovarian reserves and different body mass index (BMI) has not been studied. However, among these protocols, the one that elicits higher live birth in IVF/ICSI patients with different ages, ovarian reserves and body mass indexes (BMI) has not been identified.

Methods: This was a retrospective cohort study about 8579 women who underwent the first IVF-ET from January, 2018 to August, 2021. Propensity Score Matching (PSM) was used to improve the comparability between two protocols.

Results: After PSM, significant higher live birth rates were found in the GnRH agonist long-acting protocol compared to GnRH antagonist protocol (44.04% vs. 38.32%) (p<0.001). Stratified analysis showed that for those with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m and were aged ≥ 30 years old, and for those women with BMI < 24kg/m and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol was more likely to elicit live births [OR (95%CI), 2.13(1.19,3.80)], [OR (95%CI), 1.41(1.05,1.91)]. However, among women with BMI ≥ 24kg/m and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol had a lower possibility of eliciting live births [OR (95%CI), 0.54(0.32,0.90)]. Also, among women with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m and with age < 30 years and for those with AMH levels between 3 ng/ml and 6 ng/ml, regardless of age, and with BMI<24kg/m2,, the possibility of live births was similar between the two protocols [OR (95%CI), 1.06(0.60,1.89)], [OR (95%CI), 1.38(0.97,1.97)], [OR (95%CI), 0.99(0.72,1.37)]. Among the women with AMH levels ≤ 3 ng/ml and with were aged < 30years, regardless of BMI, the possibility of live birth was similar between the two protocols [OR (95%CI), 1.02(0.68,1.54)], [OR (95%CI), 1.43(0.68,2.98)]. Moreover, among women with AMH levels ≥ 6ng/ml, the possibility of live birth was similar between the two protocols [OR (95%CI),1.42(0.75,2.69)], [OR (95%CI),1.02(0.19,5.35)], [OR (95%CI), 1.68(0.81,3.51)], [OR (95%CI), 0.51(0.10,2.55)].

Conclusions: The suitability of the GnRH agonist long-acting protocol or GnRH antagonist protocol to infertility patients is dependent on specific biological characteristics of the patients.
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http://dx.doi.org/10.3389/fendo.2022.899000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355571PMC
August 2022

Assembly of high-quality genomes of the locoweed Oxytropis ochrocephala and its endophyte Alternaria oxytropis provides new evidence for their symbiotic relationship and swainsonine biosynthesis.

Mol Ecol Resour 2022 Aug 6. Epub 2022 Aug 6.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, China.

Locoweeds are perennial forbs poisonous to livestock and cause extreme losses to animal husbandry. Locoweed toxicity is attributed to the symbiotic endophytes in Alternaria sect. Undifilum, which produce a mycotoxin swainsonine (SW). We performed a de novo whole genome sequencing of the most common locoweed in China, Oxytropis ochrocephala (2n = 16), and assembled a high-quality, chromosome-level reference genome. Its genome size is 958.83 Mb with 930.94 Mb (97.09 %) anchored and oriented onto 8 chromosomes, and 31,700 protein-coding genes were annotated. Phylogenetic and collinearity analysis showed it is closely related to Medicago truncatula with a pair of large interchromosomal rearrangements, and both species underwent a whole-genome duplication event. We also derived the genome of A. oxytropis at 74.48 Mb with a contig N50 of 8.87 Mb and 10,657 protein-coding genes, and refined the genes of SW biosynthesis. Multiple Alternaria species containing the swnK gene were grouped into a single clade, but in other genera, swnK's homologues are diverse. Resequencing of 41 A. oxytropis strains revealed one SNP in the SWN cluster causing changes in SW concentration. Comparing the transcriptomes of symbiotic and non-symbiotic interactions identified differentially expressed genes (DEG) linked to defense and secondary metabolism in the host. Within the endophyte DEGs were linked to cell wall degradation, fatty acids and nitrogen metabolism. Symbiosis induced the up-regulation of most of the SW biosynthetic genes. These two genomes and relevant sequencing data should provide valuable genetic resources for the study of the evolution, interaction, and SW biosynthesis in the symbiont.
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http://dx.doi.org/10.1111/1755-0998.13695DOI Listing
August 2022

NXN suppresses metastasis of hepatocellular carcinoma by promoting degradation of Snail through binding to DUB3.

Cell Death Dis 2022 Aug 4;13(8):676. Epub 2022 Aug 4.

State Key Laboratory of Oncology in South China and Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, China.

The poor prognosis of hepatocellular carcinoma (HCC) could be attributed to its high metastasis rate. Here, we report the role of nucleoredoxin (NXN), a multifunctional redox-active protein, in HCC metastasis. The expression of NXN in HCC tissues was measured by immunohistochemistry. The role of NXN on HCC proliferation was determined by CCK-8, EdU and colony formation assays in vitro and subcutaneous tumor formation model in vivo. Transwell and wound healing assays and tail vein injection model were performed to assess the function of NXN on HCC metastasis. Co-immunoprecipitation assay was performed to examine the interaction among NXN, Snail and DUB3. Our results showed that NXN was downregulated in HCC tissues compared to adjacent liver tissues. Patients with low NXN expression had shorter overall survival (OS) time (P < 0.001) than those with high NXN expression. Biologically, ectopic expression of NXN significantly inhibited the proliferation and metastasis of HCC cells both in vitro and in vivo by suppressing epithelial-mesenchymal transition (EMT). Mechanistically, NXN promoted the ubiquitin-proteasome-mediated degradation of Snail through interaction with DUB3. Further, depletion of Snail abolished NXN-inhibited cell proliferation and metastasis. In summary, NXN suppressed the proliferation and metastasis of HCC by inhibiting DUB3-mediated deubiquitylation of Snail protein. Our study demonstrates that NXN, DUB3 and Snail complex functioned as an important regulatory mechanism of HCC progression and indicates a potential therapeutic approach for the treatment of HCC metastasis.
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http://dx.doi.org/10.1038/s41419-022-05135-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352874PMC
August 2022

Dermatofibrosarcoma protuberans presenting as an annulare cutaneous lesion: A case report.

Asian J Surg 2022 Jul 31. Epub 2022 Jul 31.

Department of Dermatology, GuiQian International General Hospital, No. 1, Dongfeng Street, Wudang District, Guiyang, Guizhou, 550018, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.07.013DOI Listing
July 2022

Recent advances in starch-based magnetic adsorbents for the removal of contaminants from wastewater: A review.

Int J Biol Macromol 2022 Jul 29;218:909-929. Epub 2022 Jul 29.

School of Chemistry and Chemical Engineering, School of Resources, Environment and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metallic and Featured Materials Guangxi University, Nanning 530004, Guangxi, China. Electronic address:

Considerable concern exists regarding water contamination by various pollutants, such as conventional pollutants (e.g., heavy metals and organics) and emerging micropollutants (e.g., consumer care products and interfering endocrine-related compounds). Currently, academics are continuously exploring sustainability-related materials and technologies to remove contaminants from wastewater. Magnetic starch-based adsorbents (MSAs) can combine the advantages of starch and magnetic nanoparticles, which exhibit unique critical features such as availability, cost-effectiveness, size, shape, crystallinity, magnetic properties, stability, adsorption properties, and excellent surface properties. However, limited reviews on MSAs' preparations, characterizations, applications, and adsorption mechanisms could be available nowadays. Hence, this review not only focuses on their activation and preparation methods, including physical (e.g., mechanical activation treatment, microwave radiation treatment, sonication, and extrusion), chemical (e.g., grafting, cross-linking, oxidation and esterification), and enzymatic modifications to enhance their adsorption properties, but also offers an all-round state-of-the-art analysis of the full range of its characterization methods, the adsorption of various contaminants, and the underlying adsorption mechanisms. Eventually, this review focuses on the recycling and reclamation performance and highlights the main gaps in the areas where further studies are warranted. We hope that this review will spark an interdisciplinary discussion and bring about a revolution in the applications of MSAs.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.175DOI Listing
July 2022

Study of the effects of [email protected] on metabolism in mouse RAW 264.7 macrophages.

Biomater Adv 2022 Jul 13;138:212800. Epub 2022 Apr 13.

Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Mass spectrometry-based metabolomics plays a vital role in discovering new markers and revealing the unpredictable biological effects of external stimuli. However, the current metabolomics research on materials is still in its infancy, and in-depth research on possible toxic mechanisms is lacking. In this study, a nanocomposite of gold nanoparticles (AuNPs)-zeolite-imidazole framework-8 (ZIF-8) ([email protected]) was designed to investigate its effects on metabolism in mouse RAW 264.7 macrophages. The successful synthesis of [email protected] was confirmed by transmission electron microscopy (TEM) and elemental analysis. The changes in the metabolic activity of mouse RAW 264.7 macrophages at different concentrations of [email protected] and different treatment times were investigated, and their influence on the morphological changes and behavior of RAW 264.7 cells was discussed. In addition, ultrahigh-performance liquid chromatography quadrupole-orbital high-resolution mass spectrometry (UHPLC/Q-Orbitrap HRMS) was used to study the metabolic effects of [email protected] on RAW 264.7 cells, and the results showed different metabolites being expressed at different reaction times. After 4, 8 and 24 h of treatment, the differential expression of 14, 16, and 16 metabolites, respectively, was detected. Twenty-five candidate key metabolites were identified from the results of the expression patterns and metabolic pathways. These metabolites are related to glutamine metabolism, the tricarboxylic acid cycle and glycolytic metabolic pathways, which may provide insight into the treatment of diseases caused and progressed by glutamine metabolism. This study also indicates the effectiveness of high-resolution LC-MS in revealing the nanotoxicity mechanism of [email protected]
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http://dx.doi.org/10.1016/j.bioadv.2022.212800DOI Listing
July 2022

Drainage volume on postoperative day one to predict clinically relevant postoperative pancreatic fistula following distal pancreatectomy.

BMC Surg 2022 Aug 1;22(1):297. Epub 2022 Aug 1.

Department of Nuclear Medicine, Second Affiliated Hospital of Chengdu Medical College (China National Nuclear Corporation 416 Hospital), Chengdu, Sichuan, 610000, People's Republic of China.

Background: The purpose of this study was to determine how the drain fluid volume on the first day after surgery (DFV 1) can be used to predict clinically relevant post-operative pancreatic fistula following distal pancreatectomy (DP).

Method: A retrospective analysis of 175 patients who underwent distal pancreatectomy in hepatobiliary surgery at Chengdu 363 Hospital (China) from January 2015 to January 2021 has been performed. Depending on the presence of pancreatic fistula, all patients were divided into two groups: POPF and non-POPF. The clinical factors were analyzed using SPSS 17.0 and Medcalc software. In order to assess the effectiveness of DFV 1 in predicting POPF after surgery, ROC curves were used to calculate its cut-off point,, which yielded sensitivity and negative predictive value of 100% for excluding POPF.

Result: Of the 175 patients who underwent distal pancreatectomy, the incidence of overall pancreatic fistula was 36%, but the rate of clinically significant (grade B and C) fistula, as defined by the International Study Group on Pancreatic Fistula, 30 was only 17.1% (28 grade B and 2 grade C fistula). The results from univariate and multivariate logistic regression analysis showed that drain fluid volume on the first postoperative day (OR = 0.95, P = 0.03), drainage fluid amylase level on POD1 (OR = 0.99, P = 0.01) and the preoperative ALT level (OR = 0.73, P = 0.02) were independent risk factors associated with CR-POPF. Receiver operating characteristic (ROC) curve analysis revealed that a drainage volume of 156 mL within 24 h and an amylase greater than 3219.2 U/L on the first postoperative day were the optimal thresholds associated with complications.

Conclusion: After distal pancreatectomy, the drainage volume on the first postoperative day can predict the presence of a clinically relevant pancreatic fistula.
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http://dx.doi.org/10.1186/s12893-022-01748-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341036PMC
August 2022

Suppression of circXPO1 attenuates cigarette smoke-induced inflammation and cellular senescence of alveolar epithelial cells in chronic obstructive pulmonary disease.

Int Immunopharmacol 2022 Jul 27;111:109086. Epub 2022 Jul 27.

Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai 200240, China. Electronic address:

Smoking is an essential facet of the pathogenesis of chronic obstructive pulmonary disease (COPD), which is typically characterized by inflammation and cellular senescence of alveolar epithelial cells. In this study, we investigated the function and fundamental mechanism of a novel circular RNA XPO1 (circXPO1) in cigarette smoke (CS)-induced inflammation and cellular senescence of alveolar epithelial cells. We found that circXPO1 was overexpressed in the lungs of CS-exposed mice and the CS extract (CSE)-treated alveolar epithelial cell line MLE12. Suppression of circXPO1 inhibited CSE-induced inflammatory cytokine production and cellular senescence. In vivo assays also demonstrated that circXPO1 knockdown attenuates CS-induced inflammation and senescence in the mouse lungs. Mechanistically, circXPO1 can directly bind to miR-23b-3p, preventing miR-23b-3p from binding to its target TGF-β-activated kinase 1/MAP3K7 binding protein 3 (TAB3)mRNA. In addition, under CSE conditions, miR-23b-3p overexpression recapitulated the prophylactic effects of circXPO1 knockdown. Inhibition of miR-23b-3p attenuated the function of circXPO1 knockdown in CSE-treated MLE12 cells. These results reveal that circXPO1 plays a role in the pathogenesis of COPD by modulating TAB3 through sponging miR-23b-3p.
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http://dx.doi.org/10.1016/j.intimp.2022.109086DOI Listing
July 2022

Recent development of small-molecule fluorescent probes based on phenothiazine and its derivates.

J Photochem Photobiol B 2022 Jul 18;234:112528. Epub 2022 Jul 18.

School of Pharmacy, The Air Force Medical University, Xi'an 710032, PR China. Electronic address:

Fluorescence probes, as analytical tools with the ability to perform rapid and sensitive detection of target analytes, have made outstanding contributions to environmental analysis and bioassays. Considering the expanding developments in these areas, fluorophores play a key role in the de-sign of fluorescence probes. Compared to classical fluorophores, phenothiazines with elec-tron-rich characteristics have been widely applied to construct electron donor-acceptor dyes, which exhibit outstanding performance in both fluorimetric and colorimetric analysis. In addition, these probes also exhibit the pronounced ability in both solution and solid-state, achieving portable detection for environmental analysis. In this review, we summarize recent advances in the performance of phenothiazine-based fluorescent probes for detecting various analytes, especially in cations, anions, ROS/RSS, enzyme and other small molecules. The general design rules, response mechanisms and practical applications of the probes are analyzed, followed by a discussion of exiting challenges and future research perspectives. It is hoped that this review will provide a few strategies for the development of phenothiazine-based fluorescent probes.
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http://dx.doi.org/10.1016/j.jphotobiol.2022.112528DOI Listing
July 2022

Composite indices of femoral neck strength predicts the collapse of steroid-associated osteonecrosis of the femoral head: a retrospective study.

BMC Musculoskelet Disord 2022 Jul 28;23(1):722. Epub 2022 Jul 28.

Joint Center, the Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510405, China.

Purposes: The purpose of this study was to investigate the predictive effect exerted by composite indices of femoral neck strength (compressive strength index (CSI), bending strength index (BSI) and impact strength index (ISI) on the femoral head collapse in steroid-associated ONFH patients.

Methods: Nonoperative steroid-associated osteonecrosis of the femoral head (ONFH) patients from 2017 to 2019 were selected. The patients fell into the collapsed group and the non-collapsed group according to whether the femoral head collapsed. CSI, BSI and ISI were calculated. Moreover, bone turnover markers were measured. The statistical analysis was conducted on the predictive effects of composite indices of femoral neck strength and bone turnover index on ONFH collapse.

Results: A total of 62 patients were included. The mean CSI, BSI and ISI were significantly lower in the collapsed group than those in the non-collapsed group (P < 0.05). CSI, ISI,t-P1NP and β-CTx were suggested as the protective risk factors for the femoral head collapse in ONFH patients. The ISI area under the curve values was 0. 878.The mean survival time of the hips of patients with ISI greater than 0.435 was greater (P < 0.05) than that of patients with ISI less than 0.435.

Conclusion: The composite indices of femoral neck strength can predict steroid-associated ONFH femoral head collapse more effectively than the bone turnover markers. The ISI value of 0.435 is a potential cut-off value, lower than this value can predict the early collapse of steroid-associated ONFH.
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http://dx.doi.org/10.1186/s12891-022-05622-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330666PMC
July 2022

Biogeographic Patterns of Leaf Element Stoichiometry of L. in Degraded Grasslands on Inner Mongolia Plateau and Qinghai-Tibetan Plateau.

Plants (Basel) 2022 Jul 26;11(15). Epub 2022 Jul 26.

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China.

Plant leaf stoichiometry reflects its adaptation to the environment. Leaf stoichiometry variations across different environments have been extensively studied in grassland plants, but little is known about intraspecific leaf stoichiometry, especially for widely distributed species, such as L. We present the first study on the leaf stoichiometry of and evaluate its relationships with environmental variables. leaf and soil samples from 29 invaded sites in the two plateaus of distinct environments [the Inner Mongolian Plateau (IM) and Qinghai-Tibet Plateau (QT)] in Northern China were collected. Leaf C, N, P, and K and their stoichiometric ratios, and soil physicochemical properties were determined and compared with climate information from each sampling site. The results showed that mean leaf C, N, P, and K concentrations were 498.60, 19.95, 2.15, and 6.57 g kg; the average C:N, C:P, N:P, N:K and K:P ratios were 25.20, 245.57, 9.81, 3.13, and 3.21, respectively. The N:P:K-ratios in leaf might imply that its growth is restricted by K- or K+N. Moreover, the soil physicochemical properties in the -infested areas varied remarkably, and few significant correlations between leaf ecological stoichiometry and soil physicochemical properties were observed. These indicate the nutrient concentrations and stoichiometry of tend to be insensitive to variations in the soil nutrient availability, resulting in their broad distributions in China's grasslands. Besides, different homeostasis strength of the C, N, K, and their ratios in leaves across all sites were observed, which means could be more conservative in their use of nutrients improving their adaptation to diverse conditions. Moreover, the leaf C and N contents of were unaffected by any climate factors. However, the correlation between leaf P content and climate factors was significant only in IM, while the leaf K happened to be significant in QT. Besides, MAP or MAT contribution was stronger in the leaf elements than soil by using mixed effects models, which illustrated once more the relatively weak effect of the soil physicochemical properties on the leaf elements. Finally, partial least squares path modeling suggested that leaf P or K contents were affected by different mechanisms in QT and IM regions, suggesting that can adapt to changing environments by adjusting its relationships with the climate or soil factors to improve its survival opportunities in degraded grasslands.
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http://dx.doi.org/10.3390/plants11151943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370359PMC
July 2022

Effect of femoral head necrosis cystic area on femoral head collapse and stress distribution in femoral head: A clinical and finite element study.

Open Med (Wars) 2022 13;17(1):1282-1291. Epub 2022 Jul 13.

Department of Orthopedics, Guangdong Research Institute for Orthopedic & Traumatology of Chinese Medicine, Guangzhou, Guangdong 510240, China.

The purpose of this study was to investigate the effect of cystic areas of osteonecrosis of the femoral head (ONFH) on stress distribution and disease progression in the femoral head. A total of 85 patients (106 hips) diagnosed with Association Research Circulation Osseous stage II non-traumatic and non-surgical treatment were retrospectively analyzed. The presence of cystic areas and diameter of cystic areas were compared between the two groups. In addition, five spherical cystic areas of different diameters were constructed and the maximum stress was observed. There was a difference between the two groups in whether cystic areas appeared in the femoral head, with 49.1% in the collapse group showing cystic areas, which was significantly higher than that in the non-collapse group (18.4%) ( < 0.05). In addition, the diameter of the cystic areas was significantly larger in the collapsed group than in the non-collapsed group ( < 0.05). The maximum and mean von Mises stress value around the necrotic area and around the cystic area of the femoral head increased with the increase of the cystic diameter. Stress concentration areas can be generated around the cystic areas. The presence and increased diameter of the cystic areas accelerates the collapse of the ONFH femoral head.
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http://dx.doi.org/10.1515/med-2022-0506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281584PMC
July 2022

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes.

Plants (Basel) 2022 Jul 20;11(14). Epub 2022 Jul 20.

State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.

Maize is a cold-sensitive crop, and it exhibits severe retardation of growth and development when exposed to cold snaps during and right after seedling emergence. Although different agronomic, physiological, and molecular approaches have been tried to overcome the problems related to cold stress in recent years, the mechanisms causing cold resistance in maize are still unclear. Screening and breeding of varieties for cold resistance may be a sustainable option to boost maize production under low-temperature environments. Herein, seedlings of 39 different maize genotypes were treated under both 10 °C low temperature and 22 °C normal temperature conditions for 7 days, to assess the changes in seven growth parameters, two membrane characteristics, two reactive oxygen species (ROS) levels, and four antioxidant enzymes activities. The changes in ten photosynthetic performances, one osmotic substance accumulation, and three polyamines (PAs) metabolisms were also measured. Results indicated that significant differences among genotypes, temperature treatments, and their interactions were found in 29 studied traits, and cold-stressed seedlings were capable to enhance their cold resistance by maintaining high levels of membrane stability index (66.07%); antioxidant enzymes activities including the activity of superoxide dismutase (2.44 Unit g protein), peroxidase (1.65 Unit g protein), catalase (0.65 μM min g protein), and ascorbate peroxidase (5.45 μM min g protein); chlorophyll (Chl) content, i.e., Chl a (0.36 mg g FW) and Chl b (0.40 mg g FW); photosynthetic capacity such as net photosynthetic rate (5.52 μM m s) and ribulose 1,5-biphosphate carboxylase activity (6.57 M m s); PAs concentration, mainly putrescine (274.89 nM g FW), spermidine (52.69 nM g FW), and spermine (45.81 nM g FW), particularly under extended cold stress. Importantly, 16 traits can be good indicators for screening of cold-resistant genotypes of maize. Gene expression analysis showed that , , , , , and involved in antioxidant enzymes activity and PAs metabolism, and these genes may be used for genetic modification to improve maize cold resistance. Moreover, seven strong cold-resistant genotypes were identified, and they can be used as parents in maize breeding programs to develop new varieties.
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http://dx.doi.org/10.3390/plants11141881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320912PMC
July 2022

Transcriptome Analysis Reveals Molecular Mechanisms under Salt Stress in Leaves of Foxtail Millet ( L.).

Plants (Basel) 2022 Jul 18;11(14). Epub 2022 Jul 18.

National Engineering Research Center for the Efficient Utilization of Soil and Fertilizer, College of Resources and Environment, Shandong Agricultural University, Tai'an 271018, China.

Foxtail millet ( L.) is an important cereal for managing future water scarcity and ensuring food security, due to its strong drought and salt stress resistance owing to its developed root system. However, the molecular responses of foxtail millet leaves to salt stress are largely unknown. In this study, seeds of 104 foxtail millet accessions were subjected to 0.17 mol·L NaCl stress during germination, and various germination-related parameters were analyzed to derive 5 salt-sensitive accessions and 13 salt-tolerant accessions. Hong Gu 2000 and Pu Huang Yu were the most salt-tolerant and salt-sensitive accessions, respectively. To determine the mechanism of the salt stress response, transcriptomic differences between the control and salt-treated groups were investigated. We obtained 2019 and 736 differentially expressed genes under salt stress in the salt-sensitive and salt-tolerant accessions, respectively. The transcription factor families bHLH, WRKY, AP2/ERF, and MYB-MYC were found to play critical roles in foxtail millet's response to salt stress. Additionally, the down-regulation of ribosomal protein-related genes causes stunted growth in the salt-sensitive accessions. The salt-tolerant accession alleviates salt stress by increasing energy production. Our findings provide novel insights into the molecular mechanism of foxtail millet's response to salt stress.
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http://dx.doi.org/10.3390/plants11141864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323065PMC
July 2022

Using Fumed Silica to Develop Thermal Insulation Cement for Medium-Low Temperature Geothermal Wells.

Materials (Basel) 2022 Jul 21;15(14). Epub 2022 Jul 21.

State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Chengdu University of Technology, Chengdu 610059, China.

During geothermal energy development, the bottom high-temperature fluid continuously exchanges heat with the upper low-temperature wellbore and the stratum during its rising process. Thermal insulation cement (TIC) can increase the outlet temperature, thus effectively reducing the heat loss of the geothermal fluid and improving energy efficiency. In this study, vitrified microbubbles (VMB) were screened out by conducting an orthogonal test of compressive strength (CS) and thermal conductivity (TC) on three inorganic thermal insulation materials (VMB, expanded perlite (EP), and fly-ash cenosphere (FAC)). Fumed silica (FS) was introduced into the cement with VMBs, as its significant decreasing effect on the TC. Moreover, a cement reinforcing agent (RA) and calcium hydroxide [CH] were added to further improve the CS of TIC at 90 °C. The fresh properties, CS, TC, hydration products, pore-size distribution, and the microstructure of the cement were investigated. As a result, a TIC with a TC of 0.1905 W/(m·K) and CS of 5.85 MPa was developed. The main conclusions are as follows: (1) Increasing the mass fraction of the thermal insulation material (TIM) is an effective method to reduce TC. (2) The CH content was reduced, but the C-S-H gel increased as FS content increased due to the pozzolanic reaction of the FS. (3) As the C-S-H gel is the main product of both the hydration and pozzolanic reactions, the matrix of the cement containing 60% FS and VMBs was mainly composed of gel. (4) The 10% RA improved the cement fluidity and increased the CS of TIC from 3.5 MPa to 5.85 MPa by promoting hydration.
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http://dx.doi.org/10.3390/ma15145087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323566PMC
July 2022

Reprogramming Metabolism of Macrophages as a Target for Kidney Dysfunction Treatment in Autoimmune Diseases.

Int J Mol Sci 2022 Jul 21;23(14). Epub 2022 Jul 21.

Department of Immunology, CAMS Key Laboratory T Cell and Cancer Immunotherapy, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, State Key Laboratory of Medical Molecular Biology, Beijing 100005, China.

Chronic kidney disease (CKD), as one of the main complications of many autoimmune diseases, is difficult to cure, which places a huge burden on patients' health and the economy and poses a great threat to human health. At present, the mainstream view is that autoimmune diseases are a series of diseases and complications caused by immune cell dysfunction leading to the attack of an organism's tissues by its immune cells. The kidney is the organ most seriously affected by autoimmune diseases as it has a very close relationship with immune cells. With the development of an in-depth understanding of cell metabolism in recent years, an increasing number of scientists have discovered the metabolic changes in immune cells in the process of disease development, and we have a clearer understanding of the characteristics of the metabolic changes in immune cells. This suggests that the regulation of immune cell metabolism provides a new direction for the treatment and prevention of kidney damage caused by autoimmune diseases. Macrophages are important immune cells and are a double-edged sword in the repair process of kidney injury. Although they can repair damaged kidney tissue, over-repair will also lead to the loss of renal structural reconstruction function. In this review, from the perspective of metabolism, the metabolic characteristics of macrophages in the process of renal injury induced by autoimmune diseases are described, and the metabolites that can regulate the function of macrophages are summarized. We believe that treating macrophage metabolism as a target can provide new ideas for the treatment of the renal injury caused by autoimmune diseases.
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http://dx.doi.org/10.3390/ijms23148024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316004PMC
July 2022
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