Publications by authors named "Wei Guo"

3,709 Publications

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Characteristics of tumor infiltrating lymphocytes in sinonasal mucosal melanoma and prognosis for patients.

Curr Probl Cancer 2022 Jun 11;46(4):100878. Epub 2022 Jun 11.

Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China. Electronic address:

The significance of tumor infiltrating lymphocytes (TILs) in melanoma has been studied for a long time, but, to date, there has been insufficient research on TILs in sinonasal mucosal melanoma. The purpose of this study was to analyze the correlation between TILs and prognosis for Chinese patients with sinonasal mucosal melanoma, and to clarify the significance of TILs in prognosis. As a retrospective cohort study, 44 cases of malignant melanoma in head and neck mucosa were studied by immunohistochemical staining. The correlation between TIL classification (immune cell infiltration types), CD3, CD4, CD8, CD20, CD45, CD56, and CD68 positive cells, disease progression and prognosis for survival was analyzed. By pairing various factors, RNA sequencing and xCell analysis were performed in another 8 patients with different prognoses to further verify the expression of immune cell subsets in these patients. Immunohistochemistry and cell counting showed that the TIL classification and content of CD3, CD4, CD8, CD20, CD45, CD56, and CD68 positive cells were independent factors influencing progression-free survival, but there was no clear correlation with overall survival. RNA sequencing and xCell immunocyte analysis further confirmed the role of TILs in the prediction of disease progression. CD8+ T cells and natural killer T cells were highly expressed in patients with no disease progression, while Th2 T cells, macrophages and M2 macrophages were highly expressed in patients with disease progression. TILs can be used to predict the prognosis for patients with sinonasal mucosal melanoma. Different degrees and distributions of immune cell infiltration influence disease progression in patients with sinonasal mucosal melanoma. Patients with a diffuse distribution and a high density of infiltrating cells have a better prognosis. A high expression of CD8+ T cells and natural killer T cells, which have an immune killing effect, are beneficial in controlling progression of the disease.
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http://dx.doi.org/10.1016/j.currproblcancer.2022.100878DOI Listing
June 2022

Correction to: An evaluation of the response to neoadjuvant chemotherapy for osteosarcoma of extremities: PERCIST versus RECIST 1.1 criteria after long-term follow-up.

Ann Nucl Med 2022 Jun 25. Epub 2022 Jun 25.

Musculoskeletal Tumor Center, Peking University People's Hospital, 11 Xizhimen South Street, Beijing, China.

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http://dx.doi.org/10.1007/s12149-022-01768-yDOI Listing
June 2022

Primary clear cell adenocarcinoma of female urinary tract: a case report and literature review.

BMC Womens Health 2022 Jun 24;22(1):251. Epub 2022 Jun 24.

Department of Urology, Xiangtan Central Hospital, Xiangtan, 411100, Hunan, China.

Background: Primary clear cell adenocarcinoma of the urethra is extremely rare, reported only in single case reports, and its histological origin is not clear. There is no standard treatment for CCAU at present, and surgery is still the main treatment for CCAU without distant metastasis.

Case Presentation: A 67-year-old female complained of gross hematuria with frequent micturition and urgency. No urethral diverticulum was found by cystoscopy or MRI, and the mass grew around the urethra. Urethral and anterior pelvic viscera resection was performed. Clear cell adenocarcinoma was confirmed by immunohistochemistry after the operation, and no recurrence or metastasis was found after one year of follow-up.

Conclusion: CCAU is very rare, and most cases originate from the urethral diverticulum and some may also originate from tissues around the urethra. For CCAU patients without distant metastasis, the main treatment is still surgery, and radiotherapy and chemotherapy can be performed for patients with distant metastasis. Gene detection may provide guidance for the precise chemotherapy of CCAU.
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http://dx.doi.org/10.1186/s12905-022-01835-6DOI Listing
June 2022

Chidamide Maintenance Therapy Following Induction Therapy in Patients With Peripheral T-Cell Lymphoma Who Are Ineligible for Autologous Stem Cell Transplantation: Case Series From China.

Front Oncol 2022 7;12:875469. Epub 2022 Jun 7.

Department of Hematology, The First Hospital of Jilin University, Changchun, China.

Objective: To assess the potential benefit of chidamide maintenance therapy after induction treatment in peripheral T-cell lymphoma (PTCL).

Materials And Methods: The clinical data of 48 transplantation-ineligible patients with different PTCL subtypes who received continuous chidamide treatment after first-line therapy were collected. Progression-free survival (PFS), overall survival (OS), and safety were analyzed.

Results: In total, 68.8% of patients were male (33/48), the median age was 59.5 years (22~80). The pathological subtypes were angioimmunoblastic T-cell lymphoma (AITL, 43.8%), anaplastic large cell lymphoma (ALCL, 16.6%), PTCL-not otherwise specified (NOS, 25%), NK/T-cell lymphoma (NKT, 14.6%). 35.4% (7/48) patients had intermediate or high risk (IPI=3~5). 20 patients (41.7%) received chidamide as a maintenance treatment after achieving a complete response (CR). 57.1% (16/28) exhibited a better response after chidamide (9 cases partial response [PR] to CR, 7 from stable disease [SD] to PR). The CR and overall response rate (ORR) were 60.4% and 93.8%, respectively. In addition, 21/21 AITL, 10/12 PTCL-NOS, and 8/8 ALCL, 6/7 NK/T exhibited CR/PR as the best response during the follow-up period. Meanwhile, the CR and ORR did not differ by age (<60 ≥60: 50.0% 70.8%, P = 0.091; and 91.7% 95.8%, P = 0.551). The median follow-up period was 12.8 months (3.0-66.6), 14 patients developed PD (29.2%), 10 of them died of lymphoma (20.8%). Totally, the 40 cases achieved CR/PR from 1st line regimen got better PFS as well as OS than the rest 8 cases (the 1-year PFS was 80.8% 46.9% and the 2-year PFS was 71.9% 46.9%, P=0.012. the 1-year OS was 89.9% 72.6% and the 2-year OS was 85.9% 48.6%, P=0.032). No patients discontinued treatment because of adverse events. The most common toxicities were neutropenia (75.0%), anemia (79.2%), thrombocytopenia (58.3%), and anorexia (45.8%), and fatigue (43.8%).

Conclusion: Chidamide maintenance therapy led to improvements of PFS and OS with a manageable safety profile in patients with PTCL. Further randomized studies are required to examine the role of chidamide maintenance therapy in PTCL.
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http://dx.doi.org/10.3389/fonc.2022.875469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209709PMC
June 2022

Identifying COVID-19 Severity-Related SARS-CoV-2 Mutation Using a Machine Learning Method.

Life (Basel) 2022 May 28;12(6). Epub 2022 May 28.

School of Life Sciences, Shanghai University, Shanghai 200444, China.

SARS-CoV-2 shows great evolutionary capacity through a high frequency of genomic variation during transmission. Evolved SARS-CoV-2 often demonstrates resistance to previous vaccines and can cause poor clinical status in patients. Mutations in the SARS-CoV-2 genome involve mutations in structural and nonstructural proteins, and some of these proteins such as spike proteins have been shown to be directly associated with the clinical status of patients with severe COVID-19 pneumonia. In this study, we collected genome-wide mutation information of virulent strains and the severity of COVID-19 pneumonia in patients varying depending on their clinical status. Important protein mutations and untranslated region mutations were extracted using machine learning methods. First, through Boruta and four ranking algorithms (least absolute shrinkage and selection operator, light gradient boosting machine, max-relevance and min-redundancy, and Monte Carlo feature selection), mutations that were highly correlated with the clinical status of the patients were screened out and sorted in four feature lists. Some mutations such as D614G and V1176F were shown to be associated with viral infectivity. Moreover, previously unreported mutations such as A320V of nsp14 and I164ILV of nsp14 were also identified, which suggests their potential roles. We then applied the incremental feature selection method to each feature list to construct efficient classifiers, which can be directly used to distinguish the clinical status of COVID-19 patients. Meanwhile, four sets of quantitative rules were set up, which can help us to more intuitively understand the role of each mutation in differentiating the clinical status of COVID-19 patients. Identified key mutations linked to virologic properties will help better understand the mechanisms of infection and will aid in the development of antiviral treatments.
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http://dx.doi.org/10.3390/life12060806DOI Listing
May 2022

Micelle Dynamic Reconstruction to Effectively Modulate the Transmission of Smart Windows.

J Phys Chem B 2022 Jun 23. Epub 2022 Jun 23.

Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China.

Micelles are extremely dynamic equilibrium aggregates. The size and shape of micelles are subject to appreciable structural fluctuations with the introduction of foreign ions, temperature, etc. The highly dynamic character has for a long time hugely attracted the interest of researchers to investigate the mechanism of micellar structure change and the dependence of their optical properties on the structure change. Herein, taking the most common sodium dodecyl sulfate (SDS) as an example, the aggregation behavior of SDS with excess K and the effect of temperature on the K/SDS mixed system were detailed and systematically investigated by combining with molecular dynamics simulations and experiments. The addition of K leads to a reconfiguration of the original micelle structure, resulting in a significant change in micelle size from the nanoscale up to the microscale. And simultaneously, temperature can induce a dynamic process of conjugation/deconjugation of K/SDS micelles in the mixed solution, which is manifested macroscopically by the change of transmittance. Finally, a temperature-responsive smart gel was prepared by introducing K/SDS into a polyacrylamide (PAM) gel, which showed an excellent tunable performance in transmittance (Δ = 60.1%, Δ = 42.72%). The designed smart window shows potential applications in room temperature control (Δ = 4.1 °C) and excellent stability over the course of 50 cycles.
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http://dx.doi.org/10.1021/acs.jpcb.2c01383DOI Listing
June 2022

Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe.

Biosensors (Basel) 2022 May 28;12(6). Epub 2022 May 28.

College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.

In this paper, a novel, accurate, sensitive and rapid ratiometric fluorescent sensor was fabricated using a copper [email protected] coordination polymer (ICP), specifically, terbium ion-guanosine 5'-disodium (Cu [email protected]) nanocomposites as the ratiometric fluorescent probe, to detect alkaline phosphatase (ALP) in water. The fluorescence probe was characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The experimental results showed that, compared with Tb-GMP fluorescent sensors, Cu ratiometric fluorescent sensors based on NCs encapsulated in Tb-GMP had fewer experimental errors and fewer false-positive signals and were more conducive to the sensitive and accurate detection of ALP. In addition, the developed fluorescent probe had good fluorescence intensity, selectivity, repeatability and stability. Under optimized conditions, the ratiometric fluorescent sensor detected ALP in the range of 0.002-2 U mL (R = 0.9950) with a limit of detection of 0.002 U mL, and the recovery of ALP from water samples was less than 108.2%. These satisfying results proved that the ratiometric fluorescent probe has good application prospects and provides a new method for the detection of ALP in real water samples.
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http://dx.doi.org/10.3390/bios12060372DOI Listing
May 2022

Preclinical platforms to study therapeutic efficacy of human γδ T cells.

Clin Transl Med 2022 Jun;12(6):e814

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Background: Gamma delta (γδ) T lymphocytes are promising candidate for adoptive T cell therapy, however, their treatment efficacy is not satisfactory. Vδ2 T cells are unique to primates and few suitable models are available to assay their anti-tumour function.

Methods: We tested human γδ T cell activation, tumour infiltration, and tumour-killing in four three-dimensional (3D) models, including unicellular, bicellular and multicellular melanoma spheroids, and patient-derived melanoma organoids. We studied the effects of checkpoint inhibitors on γδ T cells and performed a small molecule screen using these platforms.

Results: γδ T cells rapidly responded to melanoma cells and infiltrated melanoma spheroids better than αβ T cells in PBMCs. Cancer-associated fibroblasts (CAFs) in bicellular spheroids, stroma cells in multicellular melanoma spheroids and inhibitory immune cells in organoids significantly inhibited immune cell infiltrates including γδ T cells and lessened their cytotoxicity to tumour cells. Tumour-infiltrating γδ T cells showed exhausted immunophenotypes with high checkpoints expression (CTLA-4, PD-1 and PD-L1). Immune checkpoint inhibitors increased γδ T cell infiltration of 3D models and killing of melanoma cells in all four 3D models. Our small molecule screen assay and subsequent mechanistic studies demonstrated that epigenetic modifiers enhanced the chemotaxis and cytotoxicity of γδ T cells through upregulating MICA/B, inhibiting HDAC6/7 pathway and downregulating the levels of PD-L1 and PD-L2 in CAFs and tumour cells. These compounds increased CXCR4 and CD107a expression, IFN-γ production and decreased PD-1 expression of γδ T cells.

Conclusions: Tumour-infiltrating γδ T cells show exhausted immunophenotypes and limited anti-tumour capacity in melanoma 3D models. Checkpoint inhibitors and epigenetic modifiers enhance anti-tumour functions of γδ T cells. These four 3D models provided valuable preclinical platforms to test γδ T cell functions for immunotherapy.
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http://dx.doi.org/10.1002/ctm2.814DOI Listing
June 2022

One-Pot Self-Assembly of Dual-Color Domes Using Mono-Sized Silica Nanoparticles.

Nano Lett 2022 Jun 22. Epub 2022 Jun 22.

Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong (SAR), China.

Spots with dual structural colors on the skin of some organisms in nature are of tremendous interest due to the unique function of their dye-free colors. However, imitation of them requires complicated manufacturing processes, expensive equipment, and multiple predesigned building blocks. In this work, a one-pot strategy based on the phase-separation-assisted nonuniform self-assembly of monosized silica nanoparticles is developed to construct domes with dual structural colors. In drying poly(ethylene glycol)-dextran-based (PEG-DEX) droplets, monosized nanoparticles distribute nonuniformly in two compartments due to the droplet inner flow and different nanoparticle compatibility with the two phases. The dome colors are derived from the self-assembled nanoparticles and are programmable by regulating the assembly conditions. The one-pot strategy enables the preparation of multicolor using only one type of building block. With the dual-color domes, encrypted patterns with a high volume of contents are designed, showing promising applications in information delivery.
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http://dx.doi.org/10.1021/acs.nanolett.2c01090DOI Listing
June 2022

Metabolomics and integrated network pharmacology analysis reveal SNKAF decoction suppresses cell proliferation and induced cell apoptisis in hepatocellular carcinoma via PI3K/Akt/P53/FoxO signaling axis.

Chin Med 2022 Jun 20;17(1):76. Epub 2022 Jun 20.

The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, Guangdong, People's Republic of China.

Background: There is no comprehensive treatment method for hepatocellular carcinoma (HCC); hence, research and development are still focused on systemic therapies, including drugs. Sinikangai fang (SNKAF) decoction, a classic Chinese herbal prescription, has been widely used to treat liver cancer. However, there is no research on its core active component and target.

Methods: Mouse models were established to measure the anticancer effect of SNKAF decoction on HCC. Further, we investigated the effect of SNKAF decoction on inhibition of hepatoma cells proliferation using cell viability, cloning and invasion assays in vitro. The components of SNKAF were collected from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and [email protected] database. Metabolomic analysis was used to identify the potential genes and pathways in HCC treated with SNKAF decoction. Then, the expression of phosphoinositide 3-kinase (PI3K), Akt, P53, FoxO proteins of the potential signal pathways were detected using Western blot.

Results: The animal experiments showed that SNKAF decoction inhibited tumor growth (P < 0.05) and induced no weight loss in the mice. In vitro data showed that HCCLM3 and MHCC97H cell proliferation was inhibited by SNKAF serum in a time- and concentration dependent manner. Further combined analysis network pharmacology with metabonomics showed that 217 target genes overlapped. The core target genes included BCL2, MCL1, Myc, PTEN, gsk3b, CASP9, CREB1, MDM2, pt53 and CCND1. Cancer-associated pathways were largely involved in SNKAF mechanisms, including P53, FoxO, and PI3K/Akt signaling pathways, which are closely related to induced-tumor cell apoptosis. In addition, Western bolt verified that 10% SNKAF serum significantly affected the main proteins of PI3K/Akt/P53/FoxO signaling pathway in both cell lines.

Conclusion: SNKAF decoction-containing serum inhibited HCCLM3 and MHCC97H cell proliferation, migration, invasion, and induced-tumor cell apoptosis in-vivo. We confirmed that SNKAF decoction is a promising alternative treatments for HCC patients.
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http://dx.doi.org/10.1186/s13020-022-00628-1DOI Listing
June 2022

Genome Sequencing of Provides Novel Insight Into Its Volatile Component Biosynthesis.

Front Plant Sci 2022 1;13:904178. Epub 2022 Jun 1.

School of Laboratory Medicine, Chengdu Medical College, Chengdu, China.

As an important economic and medicinal crop, is rich in volatile oils and widely used in food additives, essential oils, and traditional Chinese medicine. However, the lack of the genome remains a limiting factor for understanding its medicinal properties at the molecular level. Here, based on 288.72 Gb of PacBio long reads and 105.45 Gb of Illumina paired-end short reads, we assembled a draft genome for (2.70 Gb in size, contig N50 of 2.45 Mb). Approximately 90.07% of the predicted genes were annotated in public databases. Based on comparative genomic analysis, genes involved in secondary metabolite biosynthesis, flavonoid metabolism, and terpenoid biosynthesis showed significant expansion. Notably, the , , and genes, which participate in rate-limiting steps for terpenoid backbone biosynthesis and modification, may form the genetic basis for essential oil formation in . The assembled draft genome provides a valuable genetic resource for understanding the unique features of this plant and for further evolutionary and agronomic studies of Zingiberaceae species.
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http://dx.doi.org/10.3389/fpls.2022.904178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198571PMC
June 2022

Application of the Concept of Enhanced Recovery after Surgery in Total Laparoscopic Radical Gastrectomy.

J Healthc Eng 2022 14;2022:5390182. Epub 2022 Mar 14.

Department of Dermatology, the First Affiliated Hospital of Soochow University, Suzhou, China.

To explore the clinical effects of total laparoscopic radical gastrectomy under the guidance of the concept of enhanced recovery after surgery (ERAS). Fifty-five patients were perioperatively treated under the concept of ERAS (ERAS group), while the remaining 55 patients were treated under the traditional perioperative concept (control group). The operation time, intraoperative blood loss, the time of first anal exhaust and first postoperative off-bed activity, postoperative length of stay, and incidence of postoperative complications were recorded in both groups. The pain of patients was assessed using VAS system. The nausea and vomiting and abdominal distension were assessed using the NVDS and abdominal distension score, respectively, within 24 h after operation. The patient's daily living ability was evaluated by the ADL scale at 3 d after the operation. The time of first anal exhaust, the time of first postoperative off-bed activity time, and the postoperative in-hospital time were all significantly shorter in the ERAS group than those in the control group ( < 0.001). The VAS score in the ERAS group was significantly lower than that in the control group at 12 h, 24 h, 48 h, and 72 h after operation ( < .001). The ERAS group had significantly lower NVDS score and abdominal distension score than the control group ( < 0.001). The postoperative ADL score in the ERAS group was significantly higher than that in the control group ( < 0.001). ERAS during the perioperative period of total laparoscopic radical gastrectomy can promote the postoperative rehabilitation of patients and alleviate postoperative pain and gastrointestinal reactions, which is safe and effective.
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http://dx.doi.org/10.1155/2022/5390182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201709PMC
June 2022

Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1.

Clin Exp Metastasis 2022 Jun 17. Epub 2022 Jun 17.

Laboratory of Pathology, Hebei Cancer Institute, The Fourth Hospital of Hebei Medical University, Jiankang Rd. 12, Shijiazhuang, 050011, Hebei, China.

Aberrant expression of long non-coding RNAs (lncRNAs) plays pivotal roles in tumorigenesis of human malignant cancers, including esophageal squamous cell carcinoma (ESCC). However, the specific role of lncRNA NRSN2-AS1 in ESCC has not been investigated. Our analysis of clinical data revealed that NRSN2-AS1 was upregulated in ESCC tissues and negatively correlated with patient survival. Luciferase reporter assays and chromatin immunoprecipitation assays demonstrated that NRSN2-AS1 is transcribed by SOX2. In vitro functional experiments showed that NRSN2-AS1 can promote ESCC cell proliferation, migration, and invasion. Furthermore, NRSN2-AS1-binding proteins were detected using RNA pull-down assays and mass spectrometry. Mechanistically, NRSN2-AS1 can bind to phosphoglycerate kinase 1 (PGK1) and upregulate its protein levels by inhibiting its ubiquitination. Knockdown of PGK1 in part abolished the NRSN2-AS1 overexpression-induced effects on ESCC cell proliferation, migration, invasion, and epithelial‑mesenchymal transition (EMT). Thus, NRSN2-AS1 may be a diagnostic biomarker or treatment target for ESCC.
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http://dx.doi.org/10.1007/s10585-022-10174-7DOI Listing
June 2022

The Role of CD4CD8 T Cells in HIV Infection With Tuberculosis.

Front Public Health 2022 27;10:895179. Epub 2022 May 27.

Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China.

Background: Tuberculosis (TB) is an important opportunistic infection in acquired immunodeficiency diseases (AIDS). Although the frequency of CD4CD8 double-positive (DP) T cells has been observed to increase in pathological conditions, their role (phenotypic and functional) is poorly described, especially in human immunodeficiency virus (HIV) infection with TB (HIV/TB (HT) coinfection).

Methods: The percentage and phenotypic and functional properties of peripheral blood DP T cells in patients with HT coinfection in comparison to uninfected controls and to patients with HIV or TB mono-infection were analyzed by direct intracellular cytokine staining (ICS).

Results: Total and CD4CD8 DP T cells were significantly increased in patients with both HIV and TB mono-infection, especially in patients with HT coinfection. Compared with healthy controls (HCs), the percentage of DP T cells expressing chemokine receptor 5 (CCR5) in patients with HT coinfection was significantly higher. Compared with HCs and patients with TB, a lower percentage of tumor necrosis factor α (TNF-α) secreting DP T cells and a higher percentage of granzyme A-secreting DP T cells were observed in patients with HIV mono-infection and HT coinfection, respectively. In addition, DP T cells expressed more cytolytic markers (granzyme A and perforin) than CD4 T cells, but similarly to CD8 T cells in patients with HT coinfection.

Conclusions: Our data suggested that HT coinfection resulted in a marked increase in DP T cells, especially the CD4CD8 subpopulation. DP T cells may be susceptible to HT coinfection, and have the same cytotoxic function as CD8 T cells.
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http://dx.doi.org/10.3389/fpubh.2022.895179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195591PMC
June 2022

A Nomogram for Predicting Survival in Patients With Colorectal Cancer Incorporating Cardiovascular Comorbidities.

Front Cardiovasc Med 2022 27;9:875560. Epub 2022 May 27.

Chinese Academy of Medical Sciences and Peking Union Medical College, Fuwai Hospital, National Center for Cardiovascular Diseases, Beijing, China.

Background: Cardiovascular comorbidities (CVCs) affect the overall survival (OS) of patients with colorectal cancer (CRC). However, a prognostic evaluation system for these patients is currently lacking.

Objectives: This study aimed to develop and validate a nomogram, which takes CVCs into account, for predicting the survival of patients with CRC.

Methods: In total, 21,432 patients with CRC were recruited from four centers in China between January 2011 and December 2017. The nomogram was constructed, based on Cox regression, using a training cohort (19,102 patients), and validated using a validation cohort (2,330 patients). The discrimination and calibration of the model were assessed by the concordance index and calibration curve. The clinical utility of the model was measured by decision curve analysis (DCA). Based on the nomogram, we divided patients into three groups: low, middle, and high risk.

Results: Independent risk factors selected into our nomogram for OS included age, metastasis, malignant ascites, heart failure, and venous thromboembolism, whereas dyslipidemia was found to be a protective factor. The c-index of our nomogram was 0.714 (95% : 0.708-0.720) in the training cohort and 0.742 (95% : 0.725-0.759) in the validation cohort. The calibration curve and DCA showed the reliability of the model. The cutoff values of the three groups were 68.19 and 145.44, which were also significant in the validation cohort ( < 0.001).

Conclusion: Taking CVCs into account, an easy-to-use nomogram was provided to estimate OS for patients with CRC, improving the prognostic evaluation ability.
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http://dx.doi.org/10.3389/fcvm.2022.875560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196079PMC
May 2022

Transcription factor TERF1 promotes seed germination under osmotic conditions by activating gibberellin acid signaling.

Plant Sci 2022 Jun 13;322:111350. Epub 2022 Jun 13.

Graduate School of Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South St., Haidian District, Beijing 100081, PR China. Electronic address:

Seed germination is the first step of seedling establishment, which is particularly sensitive to drought stress. Elucidating the mechanism regulating seed germination under drought stress is of great importance. We showed that overexpressing Tomato Ethylene Responsive Factor 1 (TERF1), an ERF transcription factor in the ethylene signaling pathway, significantly reduced seed sensitivity to mannitol treatment during seed germination. Germination assay demonstrated that TERF1 could activate gibberellin acid (GA) signaling pathway independent on GA metabolism during germination. By comparative transcriptome analysis (mannitol vs normal germination condition, mannitol vs mannitol plus paclobutrazol (PAC, an inhibitor of GA biosynthesis)) we identified the genes regulated by TERF1 specifically under mannitol treatment and confirmed that TERF1 could activate GA signaling pathway independent on GA metabolism, which were consistent with the germination assay with mannitol and mannitol plus PAC treatment. Based on sugar, gene expression and germination analysis we proved that TERF1 promoted seed germination through glucose signaling pathway mediated by GA. Thus our study provides an underlying mechanism for activating GA signaling pathway by TERF1 during seed germination under osmotic conditions.
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http://dx.doi.org/10.1016/j.plantsci.2022.111350DOI Listing
June 2022

The effects of a virtual reality rehabilitation task on elderly subjects: An experimental study using multimodal data.

IEEE Trans Neural Syst Rehabil Eng 2022 Jun 16;PP. Epub 2022 Jun 16.

Ageing populations are becoming a global issue. Against this background, the assessment and treatment of geriatric conditions have become increasingly important. This study draws on the multisensory integration of virtual reality (VR) devices in the field of rehabilitation to assess brain function in young and old people. The study is based on multimodal data generated by combining high temporal resolution electroencephalogram (EEG) and subjective scales and behavioural indicators reflecting motor abilities. The phase locking value (PLV) was chosen as an indicator of functional connectivity (FC), and six brain regions, namely LPFC, RPFC, LOL, ROL, LMC and RMC, were analysed. The results showed a significant difference in the alpha band on comparing the resting and task states in the younger group. A significant difference between the two states in the alpha and beta bands was observed when comparing task states in the younger and older groups. Meanwhile, this study affirms that advancing age significantly affects human locomotor performance and also has a correlation with cognitive level. The study proposes a novel accurate and valid assessment method that offers new possibilities for assessing and rehabilitating geriatric diseases. Thus, this method has the potential to contribute to the field of rehabilitation medicine.
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http://dx.doi.org/10.1109/TNSRE.2022.3183686DOI Listing
June 2022

Correlation of vascular endothelial growth factor with survival and pathological characteristics of patients with osteosarcoma: A systematic review and meta-analysis.

Authors:
Sun Xin Guo Wei

Eur J Cancer Care (Engl) 2022 Jun 16:e13629. Epub 2022 Jun 16.

Orthopedic Oncology, Peking University People's Hospital, Beijing, China.

Objective: This study aimed to assess the prognostic role of vascular endothelial growth factor (VEGF) expression in osteosarcoma.

Methods: Systematic searches of PubMed, Embase, CINAHL, Cochrane Library, ScienceDirect, and Web of Science were conducted. The correlation between VEGF expression and patients' survival was our primary endpoint. The secondary endpoints were the associations between VEGF level and patients' sociodemographic and pathological characteristics. The pooled hazard ratio (HR) or odd ratio (OR) and corresponding 95% confidence intervals (CIs) were obtained to assess the associations between VEGF expression and the target factors. Subgroup and meta-regression analyses were conducted to explore potential factors that associated with VEGF efficacy.

Results: The combined HR suggested that a positive VEGF status has a negative impact on overall survival (OS) (HR = 2.58; 95% CI, 2.09-3.19; P < 0.0001) and disease-free survival (DFS) (HR = 2.54; 95% CI, 1.84-3.50; P < 0.0001) in patients with osteosarcoma. Meta-regression analysis ruled out the influence of cut-off value, disease stage, histological subtype, disease grade, tumour location, geographic area, publication year, and method of HR acquisition on heterogeneity. Results showed that VEGF expression was closely correlated with tumour staging, chemotherapy response, and metastasis.

Conclusion: Based on the study results, VEGF could serve as an effective biomarker of prognosis in patients with osteosarcoma. Besides, VEGF was related to increased tumour malignancy, which might help guide clinical decision-making regarding therapy and outcomes.
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http://dx.doi.org/10.1111/ecc.13629DOI Listing
June 2022

Does high body mass index influence the postoperative complications and long-term survival in patients with esophageal squamous cell carcinoma after minimally invasive esophagectomy?

Wideochir Inne Tech Maloinwazyjne 2022 Jun 16;17(2):317-325. Epub 2022 Mar 16.

Department of Thoracic Surgery, Daping Hospital, Army Medical University, Chongqing, China.

Introduction: The prognostic value of high body mass index (BMI) in patients with esophageal squamous cell carcinoma (ESCC) is still controversial.

Aim: To evaluate the impact of high BMI on postoperative complications and survival after minimally invasion esophagectomy (MIE) for ESCC patients.

Material And Methods: Three hundred and fourteen consecutive ESCC patients were used to analyze the potential association between high BMI and postoperative complications and survival.

Results: Patients were divided into two groups. There was no significant difference between high and low BMI groups in terms of postoperative complications, including respiratory disease (p = 0.8362), pneumothorax (p = 0.6058), anastomotic leakage (p = 0.8678), chylothorax (p = 0.9062), cardiovascular disease (p = 0.5763), vocal cord paresis (p = 0.8349), wound infection (p = 0.5763) and perioperative death (p = 0.7179). Patients in the high BMI group had a longer operative time (p = 0.003) and more blood loss (p = 0.002) than in the low BMI group. There was no difference in number of retrieved lymph nodes between the two groups (p = 0.728). Patients could not benefit from high BMI in overall survival (OS) (p = 0.2459). High BMI was not an independent prognostic factor for survival (p = 0.1735, HR = 0.776 and 95% CI: 0.5386-1.1180).

Conclusions: High BMI is associated with prolonged operative time and increased blood loss in MIE. However, high BMI is not associated with postoperative complications and not an independent prognostic factor for survival in ESCC patients who undergo MIE.
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http://dx.doi.org/10.5114/wiitm.2022.114526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186082PMC
June 2022

Lymphovascular invasion predicts disease-specific survival in node-negative esophageal squamous cell carcinoma patients after minimally invasive esophagectomy.

Wideochir Inne Tech Maloinwazyjne 2022 Jun 19;17(2):309-316. Epub 2022 Jan 19.

Department of Thoracic Surgery, Daping Hospital, Army Medical University, Chongqing, China.

Introduction: Lymphovascular invasion (LVI) is reported to be a potential prognostic predictor in esophageal squamous cell carcinoma (ESCC) patients.

Aim: To investigate the prognostic value of LVI in ESCC node-negative patients after minimally invasive esophagectomy (MIE).

Material And Methods: 1406 consecutive ESCC patients who underwent MIE were reviewed retrospectively. After exclusion, 880 patients were enrolled, and 298 node-negative patients were used for the further analysis. The Kaplan-Meier method was used to examine the survival difference. Univariate and multivariate analyses were performed to identify prognostic predictors.

Results: LVI was observed in 29.4% of all patients. Totally, the proportion of LVI was increased with advanced T (p < 0.01) and N (p < 0.01) stage and poor tumor differentiation (p < 0.01). In the node-negative patients, a similar result was obtained in T stage (p = 0.0252) and tumor differentiation (p = 0.0080). In survival analysis, the disease-specific survival (DSS) (p = 0.0146) rate was significantly lower in node-negative patients with LVI than in those without. The difference was absent when calculating disease-free survival (DFS) (p = 0.0796). Additionally, the presence of LVI was associated with lower DSS (p = 0.0187) but not DFS (p = 0.0785) in univariate analysis in node-negative patients. Moreover, in multivariate Cox regression analysis, the presence of LVI was identified as an independent prognostic factor only in DSS (p = 0.0496) but not in DFS (p = 0.5670) in node-negative patients.

Conclusions: LVI is associated with shorter DSS and an independent prognostic factor in ESCC node-negative patients after MIE.
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http://dx.doi.org/10.5114/wiitm.2021.112679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186087PMC
June 2022

Aortic arch aneurysm isolated by percutaneous total endovascular arch replacement.

Eur Heart J 2022 Jun 16. Epub 2022 Jun 16.

Department of Vascular and Endovascular Surgery, Chinese PLA General Hospital, Beijing 100853, China.

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http://dx.doi.org/10.1093/eurheartj/ehac326DOI Listing
June 2022

A review of the impacts of climate factors on humans' outdoor thermal perceptions.

J Therm Biol 2022 Jul 31;107:103272. Epub 2022 May 31.

School of Civil Engineering and Architecture, Southwest University of Science and Technology, China.

Outdoor thermal comfort has been studied globally in recent years to understand thermal perceptions of the local people. It was usually indicated by neutral temperature (NT). However, the findings of studies vary slightly for different areas, a factor which is rarely considered. This article reviewed the results (NTs) of some previous studies with the aim to find the causes of their differences. The calculated NTs (indicated by the physiological equivalent temperature, PET; universal thermal climate index, UTCI; and standard effective temperature*, SET*) in two extreme seasons were associated with the local climate factors (latitude & annual mean temperature, AMT) through linear regression. It was found that NTs decreased with the increase of latitude and the reduction of local AMT, and showed a more significant correlation with latitude than local AMT during summer seasons. This phenomenon occurred for all thermal indices. Increasing the latitude by 10° caused summer NT decreases of 0.23 °C (PET, R = 0.5505), 0.22 °C (UTCI, R = 0.5781), and 0.33 °C (SET*, R = 0.2952), thus proving the impacts of local climatic factors on residents' thermal feelings. This effect may be the result of subjective adaptation towards local permanent thermal conditions. The findings of this study provide directions for further thermal services against people from different climatic zones.
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http://dx.doi.org/10.1016/j.jtherbio.2022.103272DOI Listing
July 2022

Letter to the Editor: An ultra-sensitive assay using cell-free DNA fragmentomics for multi-cancer early detection.

Mol Cancer 2022 Jun 11;21(1):129. Epub 2022 Jun 11.

Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Early detection can benefit cancer patients with more effective treatments and better prognosis, but existing early screening tests are limited, especially for multi-cancer detection. This study investigated the most prevalent and lethal cancer types, including primary liver cancer (PLC), colorectal adenocarcinoma (CRC), and lung adenocarcinoma (LUAD). Leveraging the emerging cell-free DNA (cfDNA) fragmentomics, we developed a robust machine learning model for multi-cancer early detection. 1,214 participants, including 381 PLC, 298 CRC, 292 LUAD patients, and 243 healthy volunteers, were enrolled. The majority of patients (N = 971) were at early stages (stage 0, N = 34; stage I, N = 799). The participants were randomly divided into a training cohort and a test cohort in a 1:1 ratio while maintaining the ratio for the major histology subtypes. An ensemble stacked machine learning approach was developed using multiple plasma cfDNA fragmentomic features. The model was trained solely in the training cohort and then evaluated in the test cohort. Our model showed an Area Under the Curve (AUC) of 0.983 for differentiating cancer patients from healthy individuals. At 95.0% specificity, the sensitivity of detecting all cancer reached 95.5%, while 100%, 94.6%, and 90.4% for PLC, CRC, and LUAD, individually. The cancer origin model demonstrated an overall 93.1% accuracy for predicting cancer origin in the test cohort (97.4%, 94.3%, and 85.6% for PLC, CRC, and LUAD, respectively). Our model sensitivity is consistently high for early-stage and small-size tumors. Furthermore, its detection and origin classification power remained superior when reducing sequencing depth to 1× (cancer detection: ≥ 91.5% sensitivity at 95.0% specificity; cancer origin: ≥ 91.6% accuracy). In conclusion, we have incorporated plasma cfDNA fragmentomics into the ensemble stacked model and established an ultrasensitive assay for multi-cancer early detection, shedding light on developing cancer early screening in clinical practice.
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http://dx.doi.org/10.1186/s12943-022-01594-wDOI Listing
June 2022

Weighted deep factorizing heterogeneous molecular network for genome-phenome association prediction.

Methods 2022 Jun 8;205:18-28. Epub 2022 Jun 8.

College of Electrical and Information Engineering, Beijing University of Civil Engineering and Architecture, Beijing, China. Electronic address:

Genome-phenome association (GPA) prediction can promote the understanding of biological mechanisms about complex pathology of phenotypes (i.e., traits and diseases). Traditional heterogeneous network-based GPA approaches overwhelmingly need to project heterogeneous data toward homogeneous network for data fusion and prediction, such projections result in the loss of heterogeneous network structure information. Matrix factorization based data fusion can avoid such projection by integrating multi-type data in a coherent way, but they typically perform linear factorization and cannot mine the nonlinear relationships between molecules, which compromise the accuracy of GPA analysis. Furthermore, most of them can not selectively synergy network topology and node attribution information in a principle way. In this paper, we propose a weighted deep matrix factorization based solution (WDGPA) to predict GPAs by selectively and differentially fusing heterogeneous molecular network and diverse attributes of nodes. WDGPA firstly assigns weights to inter/intra-relational data matrices and attribute data matrices, and performs deep matrix factorization on these matrices of heterogeneous network in a cooperative manner to obtain the nonlinear representations of different nodes. In addition, it performs low-rank representation learning on the attribute data with the shared nonlinear representations. In this way, both the network topology and node attributes are jointly mined to explore the representations of molecules and complex interplays between molecules and phenotypes. WDGPA then uses the representational vectors of gene and phenotype nodes to predict GPAs. Experimental results on maize and human datasets confirm that WDGPA outperforms competitive methods by a large margin under different evaluation protocols.
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http://dx.doi.org/10.1016/j.ymeth.2022.05.008DOI Listing
June 2022

Reprocessable Polybenzoxazine Thermosets with High Ts and Mechanical Strength Retentions Using Boronic Ester Bonds as Crosslinkages.

Polymers (Basel) 2022 May 31;14(11). Epub 2022 May 31.

Key Laboratory for Special Functional Aggregated Materials of Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.

In order to obtain reprocessable polybenzoxazine thermosets with high heat resistance and mechanical strength retentions, network structures without irreversible parts were constructed via crosslinking benzoxazine oligomers using boronic ester cross-linkers. Firstly, the benzoxazine monomer containing carbon-carbon double bonds was synthesized via the Mannich reaction. After thermal ring-opening polymerization, the benzoxazine oligomer containing carbon-carbon double bonds (OBZ) was yielded. Through the thiol-ene click reaction of the OBZ and dithiol cross-linker bearing boronic ester bonds, the polybenzoxazine thermosets using boronic ester bonds as crosslinkages (OBZ-BDB) were successfully synthesized. The structures of OBZ and OBZ-BDB were characterized by SEC, H NMR, and FT-IR measurements. Reprocessing experiments showed that OBZ-BDB has remarkable reprocessability. The retention rates of the tensile strengths through three generations of reprocessing were 98%, 95%, and 84%, respectively. Meanwhile, OBZ-BDB cross-linked by boronic ester bonds had brilliant thermal properties. The T of the original OBZ-BDB was 224 °C. With the increase of the reprocessing generations, the Ts basically remained unchanged.
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http://dx.doi.org/10.3390/polym14112234DOI Listing
May 2022

Bone Metastases of Endometrial Carcinoma Treated by Surgery: A Report on 13 Patients and a Review of the Medical Literature.

Int J Environ Res Public Health 2022 Jun 2;19(11). Epub 2022 Jun 2.

Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing 100044, China.

Background: The aim of this study was to describe the clinicopathological features of endometrial cancer (EC) patients with bone metastases treated with surgery and to systematically review the literature.

Methods: We performed a retrospective study to include patients with bone metastases of EC at Peking University People's Hospital from 2000 to 2019. Clinicopathological features and survival outcomes were collected.

Results: Among the 1662 patients with EC, 14 (0.84%) were identified with bone metastases, and all were treated surgically. Thirteen cases were analyzed. Four had bone metastases when diagnosed, and the remaining nine cases had bone metastases when first relapsed, with a median time to recurrence of 13 months (range, 5-144). The median age of the 13 patients was 58 years old (range, 45-76). Twelve were endometrioid carcinoma. The majority of sites of bone metastases were the pelvis, followed by the spine. The median overall survival (OS) was 57 months. We further combined the 13 patients with another 24 cases identified from literature research. There was no significant difference in clinicopathological characteristics between the patients with bone metastases when diagnosed and when they first relapsed. The median OS was numerically longer for patients with bone metastases when diagnosed than when they first relapsed (57 vs. 36 months, = 0.084).

Conclusions: Patients with bone metastases of EC might benefit from comprehensive treatment based on surgery, as symptoms can be palliated and survival can probably be extended.
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http://dx.doi.org/10.3390/ijerph19116823DOI Listing
June 2022

Phase II study of TQB2450, a novel PD-L1 antibody, in combination with anlotinib in patients with locally advanced or metastatic soft tissue sarcoma.

Clin Cancer Res 2022 Jun 8. Epub 2022 Jun 8.

Peking University People's Hospital, Beijing, Beijing, China.

Purpose: To explore the efficacy and safety of TQB2450 combined with anlotinib in patients with locally advanced or metastatic soft tissue sarcoma (LA/M STS).

Experimental Design: This was a single arm phase II study (TQB2450-Ib-02 study) performed at two hospitals in China to assess the potency of TQB2450 combined with anlotinib in patients with LA/M STS. Patients were previously unresponsive to at least one chemotherapy regimen. Anlotinib (12 mg QD) was administered orally from day 1 to day 14 every 3 weeks. TQB2450 was administered by intravenous infusion at 1200 mg on day 1 every 3 weeks. The primary endpoint was the objective response rate (ORR). The secondary endpoints included progression-free survival (PFS), overall survival (OS), disease control rate (DCR), and safety.

Results: Between January 2019 and June 2020, 30 patients were enrolled. The ORR was 36.67% and the DCR was 76.67%. The median PFS was 7.85 months [95% confidence interval (CI) 2.89-23.06] and the median OS was not reached (95% CI 10.58-NE). Among the patients with ASPS (12/30, 40%), the ORR was 75% and the median PFS was 23.06 months (95% CI, 8.97-NE). The most common treatment related adverse events were hypothyroidism (76.67%), hypertriglyceridemia (63.33%), hypercholesterolemia (60.00%), and elevated blood lactate dehydrogenase (53.33%).

Conclusions: The study showed the promising activity in patients with ASPS, also indicating the trend of treatment efficacy in others sarcomas. The toxicity was tolerable. More studies with larger sample size and controlled arm were warranted.
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http://dx.doi.org/10.1158/1078-0432.CCR-22-0871DOI Listing
June 2022

Expression Level Dominance and Homeolog Expression Bias Upon Cold Stress in the F1 Hybrid Between the Invasive and the Native in South China, and Implications for Its Invasiveness.

Front Genet 2022 19;13:833406. Epub 2022 May 19.

China-ASEAN College of Marine Sciences, Xiamen University Malaysia, Sepang, Malaysia.

The role of hybridization is significant in biological invasion, and thermotolerance is a trait critical to range expansions. The South American is now widespread in South China, threatening the native by competition and hybridization. Furthermore, upon formation, their F1 hybrid can quickly replace both parents. In this study, the three taxa were used as a model to investigate the consequences of hybridization on cold tolerance, particularly the effect of subgenome dominance in the hybrid. Upon chilling treatments, physiological responses and transcriptome profiles were compared across different temperature points to understand their differential responses to cold. While both parents showed divergent responses, the hybrid's responses showed an overall resemblance to but the contribution of homeolog expression bias to cold stress was not readily evident in the F1 hybrid possibly due to inherent bias that comes with the sampling location. Our findings provided insights into the role of gene expression in differential cold tolerance, and further contribute to predicting the invasive potential of other hybrids between and its congeners around the world.
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http://dx.doi.org/10.3389/fgene.2022.833406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160872PMC
May 2022

E2F1-activated NRSN2 promotes esophageal squamous cell carcinoma progression through AKT/mTOR pathway.

Pathol Res Pract 2022 May 31;236:153963. Epub 2022 May 31.

Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. Electronic address:

Background: Neurensin‑2 (NRSN2) has been reported to act as an oncogene in several types of human cancer. However, the molecular mechanism of NRSN2 in esophageal squamous cell carcinoma (ESCC) remains to be elucidated.

Methods: The mRNA expression levels of NRSN2 in ESCC tissues and cell lines were evaluated by quantitative real-time PCR (qRT-PCR). The protein expression levels of NRSN2 in ESCC tissues were measured by Immunohistochemical (IHC) method. Luciferase reporter and chromatin immunoprecipitation assays were conducted to confirm the upstream transcription factor of NRSN2. Loss- and gain-function assays were conducted to evaluate the effects of NRSN2 on ESCC cells proliferation, migration, and invasion. The function of NRSN2 was validated in vivo using tumor xenografts. The relationship between NRSN2 and AKT/mTOR pathway were confirmed by western blot assay.

Results: The expression level of NRSN2 was increased in ESCC tissues and cell lines. High expression level of NRSN2 was correlated with depth of invasion, lymph node metastasis, TNM stage, and poor prognosis of ESCC patients. NRSN2 was transcribed by E2F1. Knockdown of NRSN2 significantly inhibited ESCC cells proliferation, migration, and invasion, whereas NRSN2 overexpression showed reverse phenotypes. Overexpression of NRSN2 also enhanced ESCC tumorigenicity in vivo. Furthermore, the E2F1/NRSN2 axis promoted proliferation, migration, and invasion of ESCC cells by activating the AKT/mTOR pathway.

Conclusion: NRSN2 is a direct transcriptional target of E2F1 to promote tumor progression in ESCC. NRSN2 may be a diagnostic biomarker or treatment target for ESCC.
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http://dx.doi.org/10.1016/j.prp.2022.153963DOI Listing
May 2022
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