Publications by authors named "Wei Cai"

899 Publications

A critical look at the prediction of the temperature field around a laser-induced melt pool on metallic substrates.

Sci Rep 2021 Jun 9;11(1):12224. Epub 2021 Jun 9.

Department of Mechanical Engineering, Stanford University, Stanford, CA, 94305, USA.

The study of microstructure evolution in additive manufacturing of metals would be aided by knowing the thermal history. Since temperature measurements beneath the surface are difficult, estimates are obtained from computational thermo-mechanical models calibrated against traces left in the sample revealed after etching, such as the trace of the melt pool boundary. Here we examine the question of how reliable thermal histories computed from a model that reproduces the melt pool trace are. To this end, we perform experiments in which one of two different laser beams moves with constant velocity and power over a substrate of 17-4PH SS or Ti-6Al-4V, with low enough power to avoid generating a keyhole. We find that thermal histories appear to be reliably computed provided that (a) the power density distribution of the laser beam over the substrate is well characterized, and (b) convective heat transport effects are accounted for. Poor control of the laser beam leads to potentially multiple three-dimensional melt pool shapes compatible with the melt pool trace, and therefore to multiple potential thermal histories. Ignoring convective effects leads to results that are inconsistent with experiments, even for the mild melt pools here.
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http://dx.doi.org/10.1038/s41598-021-91039-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190039PMC
June 2021

The role of anlotinib-mediated EGFR blockade in a positive feedback loop of CXCL11-EGF-EGFR signalling in anaplastic thyroid cancer angiogenesis.

Br J Cancer 2021 Jun 4. Epub 2021 Jun 4.

Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Hypoxia-induced angiogenesis functions importantly in anaplastic thyroid cancer (ATC) progression. However, the therapeutic potential of broad-spectrum anti-angiogenic agent remains undefined. Anlotinib conventionally targets VEGFR, FGFR and PDGFR. Here, a novel role of anlotinib on ATC angiogenesis was illustrated.

Methods: Molecular expressions were established via tissue microarray. Multiple assays (tubule formation, 3D sprouting and chicken chorioallantoic membrane model) were used for angiogenic evaluation. Panels of molecular screening were achieved by antibody and PCR arrays. The loop binding motif of EGFR for homology modelling was prepared using Maestro.

Results: Anlotinib could dose- and time-dependently inhibit cell viability under normoxia and hypoxia and could repress hypoxia-activated angiogenesis more efficiently in vitro and in vivo. CXCL11 and phospho-EGFR were hypoxia-upregulated with a positive correlation. The cancer-endothelium crosstalk could be mediated by the positive CXCL11-EGF-EGFR feedback loop, which could be blocked by anlotinib directly targeting EGFR via a dual mechanism by simultaneous inhibitory effects on cancer and endothelial cells. The AKT-mTOR pathway was involved in this regulatory network.

Conclusions: The newly identified CXCL11-EGF-EGFR signalling provided mechanistic insight into the interaction between cancer and endothelial cells under hypoxia, and EGFR was a novel target. Anlotinib may be the encouraging therapeutic candidate in ATC.
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http://dx.doi.org/10.1038/s41416-021-01340-xDOI Listing
June 2021

Decoupling of wastewater eco-environmental damage and China's economic development.

Sci Total Environ 2021 May 25;789:147980. Epub 2021 May 25.

Hubei Key Laboratory of Mechanical Transmission and Manufacturing Engineering, Wuhan University of Science & Technology, Wuhan 430081, China.

Wastewater pollution has been considered as a prominent bottleneck restricting global sustainable development. China is one of the largest discharges and eco-environmental damages of wastewater in the world. Through analyzing wastewater discharge data using emergy method in China from 2011 to 2017, the wastewater eco-environmental damage of 31 provinces is calculated with GDP and area to reveal the fundamental origins of inflection point of wastewater discharge in China. Studies results show that, (i) Chinese "12th Five-Year Plan" (2011-2015) is a watershed in wastewater discharge, and the eco-environment damages caused by China's wastewater accounted for more than 1/4 of GDP; (ii) China has the great potential to reduce eco-environment damages of 1.73 trillion $/year; (iii) In 2016 and 2017, wastewater eco-environmental damage has decreased by about 50% compared with that in 2015, and the effect of government policies was remarkable. We conclude that decoupling of China's economic development form eco-environmental damages of wastewater is began to appear, the strict formulation and implementation of China's environmental policies and the green upgrading of industrial structure are main driving forces, and it is little correlation with economic slowdown. This study offers the detailed list of China wastewater pollution and reveals the relationship between wastewater eco-environmental damages and economic development, and shows the experience and achievements of the Chinese government in the treatment of wastewater pollution, which provides a useful reference for the treatment of wastewater pollution in the world.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147980DOI Listing
May 2021

Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.

J Inflamm Res 2021 24;14:2157-2172. Epub 2021 May 24.

Zhejiang Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences (Hangzhou Medical College), Hangzhou, People's Republic of China.

Purpose: Plant-derived exogenous microRNAs (miRNAs) regulate human physiological functions by blocking the translation of target mRNAs. Although several computational approaches have been developed to elucidate the interactions of cross-species miRNAs and their targets in mammals, the number of verified plant miRNAs is still limited, and the biological roles of most exogenous plant miRNAs remain unknown.

Methods: A miRNA mimic library-based phenotypic screening, which contained 8394 plant mature miRNAs published in the official database miRbase, was performed to identify more novel bioactive plant miRNAs for the prevention of hepatic fibrosis. Inhibition of candidates for the activation of hepatic stellate cells (HSCs) and the underlying mechanisms were evaluated in TGF-β1- and PDGF-exposed HSC models. The protective effects of the candidates against CCl-induced liver fibrosis were evaluated in a mouse model.

Results: Among the 8394 plant mature miRNAs reported in the official database miRBase, five candidates were found to effectively inhibit the differentiation of HSCs. gma-miR-159a (miR159a) exerted the strongest inhibitory activities on both TGF-β1- and PDGF-induced HSC activation and proliferation by inhibiting the GSK-3β-mediated NF-κB and TGF-β1 pathways. Moreover, miR159a was mainly accumulated in the liver after intravenous injection, and it reduced CCl-induced hepatic fibrosis and inflammation in mice.

Conclusion: Results indicated that miR159a has the therapeutic potential for preventing hepatic fibrosis. This study provides a novel strategy for achieving natural nucleic acid drugs.
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http://dx.doi.org/10.2147/JIR.S304828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163999PMC
May 2021

Expression level of PLAC1 in osteosarcoma patients and its regulatory effect on the development of osteosarcoma.

J BUON 2021 Mar-Apr;26(2):592-598

Department of Orthopaedics, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.

Purpose: To detect the plasma levels of PLAC1 in osteosarcoma patients, and its regulatory effect on cell proliferation and apoptosis of osteosarcoma.

Methods: Plasma levels of PLAC1 in osteosarcoma patients were examined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Receiver operating characteristics (ROC) and Kaplan-Meier curves were performed for assessing the diagnostic and prognostic potentials of PLAC1 in osteosarcoma, respectively. Moreover, the regulatory effects of PLAC1 on proliferative and apoptotic rates of osteosarcoma cells were determined through cell counting kit-8 (CCK-8) and flow cytometry, respectively.

Results: PLAC1 was highly expressed in plasma of osteosarcoma patients showing diagnostic and prognostic potentials. Overexpression of PLAC1 in U2-OS cells increased the proliferative rate but decreased the apoptotic rate, while knockdown of PLAC1 yielded the opposite results.

Conclusions: PLAC1 is upregulated in the plasma of osteosarcoma patients, serving as a diagnostic biomarker, and is unfavorable to the prognosis if this disease. PLAC1 promotes the development of osteosarcoma by stimulating cell proliferation and inhibiting apoptosis.
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June 2021

Nanoinfrared Characterization of Bilayer Graphene Conductivity under Dual-Gate Tuning.

Nano Lett 2021 Jun 1. Epub 2021 Jun 1.

The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, and TEDA Institute of Applied Physics, Nankai University, Tianjin 300457, People's Republic of China.

Dual-gate tuning on two-dimensional (2D) heterostructures can provide independent control of the carrier concentration and interlayer electrostatic potential, yielding novel electronic and optical properties. In this paper, by utilizing monolayer graphene as both the top gate and a plasmon wavelength magnifier, the optical properties of bilayer graphene (BLG) under dual-gate are quantitatively investigated by nanoinfrared imaging. The hybrid optical modes in the vertically coupled two-layer system are imaged from scattering-type scanning near-field microscopy (s-SNOM). Moreover, plasmon dispersion behaviors under varied dual-gate tuning are explored and explained well with theoretical ones employing tight binding approximation, which reveals the flexibility in individually manipulating the Fermi energy and bandgap. Especially, electron-hole asymmetry in BLG is verified from experiments. Our studies pave route for quantitative near-field investigation of superlattice, topological boundaries, and other emergent phenomena in graphene-based 2D heterostructures.
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http://dx.doi.org/10.1021/acs.nanolett.1c01167DOI Listing
June 2021

Detection of Disease-Causing SNVs/Indels and CNVs in Single Test Based on Whole Exome Sequencing: A Retrospective Case Study in Epileptic Encephalopathies.

Front Pediatr 2021 13;9:635703. Epub 2021 May 13.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Epileptic encephalopathies (EEs) are a pediatric entity with highly phenotypic and genetic heterogeneity. Both single nucleotide variants (SNVs)/Indels and copy number variations (CNVs) could be the causes. Whole exome sequencing (WES) is widely applied to detect SNVs/Indels, but the bioinformatics approach for detecting CNVs is still limited and weak. In the current study, the possibility of profiling both disease-causing SNVs/Indels and CNVs in a single test based on WES in EEs was evaluated. The infants diagnosed with EEs were enrolled from a single pediatric epilepsy center between January 2018 and February 2020. Demographic and clinical data were collected. In WES data, the pathogenic SNVs were identified through an in-house pipeline, and pathogenic CNVs were identified by CNVkit. The diagnostic rate was evaluated, and the molecular findings were characterized. A total of 73 infants were included; 36 (49.32%) of them were males. The median age was 7 months. Thirty-two (43.84%) infants had been diagnosed with epilepsy syndrome. The most common type of syndrome was West syndrome (22/73, 30.1%), followed by Dravet syndrome (20/77, 27.4%). Fifty-four (73.97%) had intellectual development delay. The genetic cause of EEs, pathogenic or likely pathogenic variants, were successfully discovered in 46.6% (34/73) of the infants, and 29 (39.7%) infants carried SNVs/Indels, while 5 (6.8%) carried CNVs. The majority of the disease-causing variants were inherited in pattern (25, 71.4%). In addition to showing that the variants in the ion channel encoding genes accounted for the main etiology, we discovered and confirmed two new disease-causing genes, and . Five discovered CNVs were deletions of 2q24.3, 1p36, 15q11-q13, 16p11.2, and 17p13.3, and all were confirmed by array comparative genomic hybridization. The application of both SNVs/Indels and CNVs detection in a single test based on WES yielded a high diagnosis rate in EEs. WES may serve as a first-tier test with cost-effective benefit in EEs.
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http://dx.doi.org/10.3389/fped.2021.635703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155357PMC
May 2021

Malignancy is a risk factor for higher COVID-19 severity:A meta-analysis.

Turk J Med Sci 2021 May 23. Epub 2021 May 23.

Background: The outbreak of coronavirus disease 2019 (COVID-19) has been an almost global pandemic with significant public health impacts. The increasing prevalence of malignancy has become a leading cause of human mortality. However, conflicting findings have been published on the association between malignancy and COVID-19 severity. This study aims to assess the pooled proportion of malignancy amongst 2019-nCov patients and to investigate the association between malignancy and COVID-19 severity.

Methods: Correlative studies were identi?ed by systematically searching electronic databases (PubMed, Web of Sciences and Embase) up to September 2, 2020. All data analyses were carried out using Stata 15.0.

Results: Twenty-nine studies consisting of 9475 confirmed COVID-19 patients (median age 54.4 years [IQR 49-62], 54.0% men) were included. The overall proportion of malignancy was 2.5% (95% CI 1.6%-3.4%). The proportion of malignancy was higher in patients with severe/critical 2019-nCoV than those in non-severe/non-critical group (3.9% [95% CI 2.0-6.3] vs 1.4% [95% CI 0.8-2.2]). Furthermore, pre-existing malignancy was associated with more than twofold higher risk of severe/critical patients with COVID-19 (OR 2.25, 95% CI 1.65-3.06 I2 = 0.0%).

Conclusion: Malignancy was associated with up to 2.3-fold higher risk of severe/critical COVID-19 and may serve as a clinical predictor for adverse outcomes.
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http://dx.doi.org/10.3906/sag-2101-192DOI Listing
May 2021

Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq.

Signal Transduct Target Ther 2021 05 17;6(1):195. Epub 2021 May 17.

School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China.

B cell response plays a critical role against SARS-CoV-2 infection. However, little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection. Here, we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients, and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses. Via linking BCR to antigen specificity through sequencing (LIBRA-seq), we identified a distinct activated memory B cell subgroup (CD11c CD95) had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells. Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection. The public antibody clonotypes were shared by distinct convalescent individuals. Moreover, several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain (RBD) or nucleoprotein (NP) via ELISA assay. Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro. Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level.
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http://dx.doi.org/10.1038/s41392-021-00610-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127497PMC
May 2021

New Methods and Technology in Drugs Metabolism and Pharmacokinetics (Part-II).

Authors:
Wei Cai Jiayu Zhang

Curr Drug Metab 2021 ;22(3):164

School of Pharmacy, Binzhou Medical University, Yantai, China.

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http://dx.doi.org/10.2174/138920022203210319085656DOI Listing
January 2021

Temporal and Spatial Dynamics of Inflammasome Activation After Ischemic Stroke.

Front Neurol 2021 22;12:621555. Epub 2021 Apr 22.

Department of Neurology, Mental and Neurological Disease Research Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

The inflammasome represents a highly pro-inflammatory mechanism. It has been identified that inflammasome was activated after ischemic stroke. However, the impact of inflammasomes on stroke outcomes remains contradictory. The participating molecules and the functioning arena of post-stroke inflammasome activation are still elusive. In the present study, blood samples from stroke patients were collected and analyzed with flow cytometry to evaluate the correlation of inflammasome activation and stroke outcomes. A stroke model was established using male C57/Bl6 mice with transient middle cerebral artery occlusion (tMCAO, 1 h). The dynamics of inflammasome components, cell type, and location of inflammasome activation and the therapeutic effects of inhibiting post-stroke inflammasome executors were evaluated. We found that a high level of inflammasome activation might indicate detrimental stroke outcomes in patients and mice models. Post-stroke inflammasome activation, especially NLRP3, cleaved Caspase-1, cleaved Caspase-11, IL-1β, IL-18, and GSDMD, peaked at 3-5 days and declined at 7 days with the participation of multiple components in mice. Macrophage that infiltrated into the ischemic lesion was the main arena for post-stroke inflammasome activation among myeloid cells according to the data of mice. Among all the members of the Caspase family, Caspase-1 and -11 served as the main executing enzymes. Inhibiting Caspase-1/-11 signaling efficiently suppressed DAMPs-induced macrophage inflammasome activation and displayed neuroprotection to stroke models including infarct size (Control: 48.05 ± 14.98; Cas1.i: 19.34 ± 12.21; Cas11.i: 21.43 ± 14.67, < 0.001) and neurological deficit score (0 d-Control: 2.20 ± 0.63; 0 d-Cas1.i: 2.20 ± 0.63; 0 d-Cas11.i: 2.20 ± 0.63; 1 d-Control: 2.50 ± 0.53; 1 d-Cas1.i: 1.50 ± 0.71; 1 d-Cas11.i: 2.00 ± 0.67; 2 d-Control: 2.30 ± 0.48; 2 d-Cas1.i: 1.30 ± 0.48; 2 d-Cas11.i: 1.50 ± 0.53; 3 d-Control: 2.00 ± 0.67; 3 d-Cas1.i: 1.20 ± 0.42; 3 d-Cas11.i: 1.30 ± 0.48, < 0.001). Taken together, inflammasome activation played a detrimental role in stroke pathology. Targeting post-stroke inflammasome executing enzymes fitting in the dynamics of macrophages might obtain potential and efficient therapeutic effects.
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http://dx.doi.org/10.3389/fneur.2021.621555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104123PMC
April 2021

Design, synthesis, and anticancer evaluation of novel andrographolide derivatives bearing an α,β-unsaturated ketone moiety.

Bioorg Chem 2021 Jul 24;112:104941. Epub 2021 Apr 24.

Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 211198, China. Electronic address:

A series of 1,2-didehydro-3-ox-andrographolide derivatives based on two Michael acceptors were designed, synthesized and evaluated for their anticancer activity against two human cancer cell lines (HCT116 and MCF-7). All tested compounds exhibited significant growth inhibitory effect on HCT116 and moderate to good inhibitory effect on MCF-7 cell proliferation. Compound 10b displayed the best inhibitory activities against both HCT116 and MCF-7 cell lines, with IC values of 2.49 and 7.80 μM respectively. Preliminary anticancer mechanistic investigation was performed in terms of the cell cycle arrest and cell apoptosis assays of compound 10b against HCT116 using flow cytometry, and the results indicated that 10b blocked the proliferation of HCT116 cells by inducing cell apoptosis in a concentration-dependent manner and arresting cell cycle in G/M phase.
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http://dx.doi.org/10.1016/j.bioorg.2021.104941DOI Listing
July 2021

Identification of Metabolites of Aurantio-Obtusin in Rats Using Ultra-High-Performance Liquid Chromatography-Q-Exactive Orbitrap Mass Spectrometry with Parallel Reaction Monitoring.

J Anal Methods Chem 2021 15;2021:6630604. Epub 2021 Apr 15.

School of Pharmaceutical Sciences, Hunan Province Key Laboratory of Antiboby-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, China.

Aurantio-obtusin (AO) is a major anthraquinone compound isolated from or , which possesses diverse pharmacological effects. Previous studies have shown that it has a good effect on lowering blood lipids and treating various diseases. A few studies have also reported about its metabolites. A rapid and reliable method using ultra-high-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry and multiple data-processing technologies was established to investigate the metabolites of AO in the plasma and various tissues of rats, including the heart, liver, spleen, lung, kidneys, and brain. Finally, a total of 36 metabolites were identified in the plasma of rats, which could be very beneficial for understanding the effective form of AO metabolites leading to new drug discovery. The result demonstrated that this strategy, especially parallel reaction monitoring, has shown a wide range of applications in the identification of metabolites.
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http://dx.doi.org/10.1155/2021/6630604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062173PMC
April 2021

A nonbile acid farnesoid X receptor agonist tropifexor potently inhibits cholestatic liver injury and fibrosis by modulating the gut-liver axis.

Liver Int 2021 Apr 24. Epub 2021 Apr 24.

Division of Pediatric Gastroenterology and Nutrition, Xin Hua hosiptal, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background & Aims: Tropifexor (TXR) is a novel nonbile acid that acts as an agonist of farnesoid X receptor (FXR). TXR is currently in Phase 2 trials for the treatment of non-alcoholic steatohepatitis (NASH). Herein, we report the impact of TXR on in a piglet model in which cholestatic liver damage and fibrosis where induced by bile duct ligation (BDL).

Methods: The piglets received BDL and TXR for 2 wk. Hepatic, portal and colonic bile acid and amino acid profiles and gut microbiome were analysed. Portal fibroblast growth factor (FGF) 19 levels were measured using an enzyme-linked immunosorbent assay (ELISA).

Results: We first showed that bile acid metabolism and signalling are dysfunctional in patients with biliary atresia. Next, we observed that TXR potently suppresses BDL-induced liver injury, fibrosis and ductular reaction in piglets. Within the ileum, TXR enhances FGF19 expression and subsequently increases portal FGF19 levels. In the liver, TXR promotes the expression of small heterodimer partner (SHP) and inhibits cholesterol 7α-hydroxylase (CYP7A1). Additionally, TXR increases the abundance of bile acid-biotransforming bacteria in the distal ileum and alters the composition of amino acids in the colon. Lastly, TXR ameliorates intestinal barrier injury in piglets subjected to BDL.

Conclusion: TXR potently ameliorated cholestatic liver injury and fibrosis by modulating the gut-liver axis in piglets. It supports the clinical evaluation of TXR as a therapeutic strategy for cholestatic liver diseases, such as biliary atresia.
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http://dx.doi.org/10.1111/liv.14906DOI Listing
April 2021

Peginterferon and Entecavir Combination Therapy Improves Outcome of Non-Early Response Hepatitis B e Antigen-Positive Patients.

Open Forum Infect Dis 2020 Nov 30;7(11):ofaa462. Epub 2020 Sep 30.

Department of Infectious Disease, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: The efficacy of nucleot(s)ide analogs (NAs) and pegylated interferon (PegIFN) combination therapy for hepatitis B e antigen-positive (HBeAg) patients is still controversial. Whether PegIFN and entecavir (ETV) combination therapy could provide a greater benefit for HBeAg patients was assessed.

Methods: Treatment-naïve HBeAg patients initiated on PegIFN alfa-2a (PegIFNα-2a) for 24 weeks without early response (early response: HBsAg <1500 IU/mL and hepatitis B virus [HBV] DNA <10 copies/mL) were recruited in the current study. Among total of 94 patients, 51 were continued on PegIFNα-2a monotherapy, and 43 were offered PegIFNα-2a and ETV combined therapy.

Results: Better outcomes in response to the combined therapy, compared with that of the monotherapy, were demonstrated, including more HBsAg decline and loss and HBV DNA decline and HBeAg clearance. Importantly, the patients with HBsAg levels between 1500 and 20000 IU/mL initially or between 5000 and 20000 IU/mL after 24 weeks of PegIFNα-2a benefitted more from the combined therapy, compared with those on monotherapy.

Conclusions: Combined therapy of PegIFNα-2a and ETV is more efficacious for HBeAg patients without early response to PegIFN monotherapy, and HBsAg levels are a good predictor of treatment outcomes.
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http://dx.doi.org/10.1093/ofid/ofaa462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050793PMC
November 2020

Carbon dioxide embolism with severe hypotension as an initial symptom during laparoscopy: a case report.

J Int Med Res 2021 Apr;49(4):3000605211004765

Department of Anesthesiology, The First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, Zhejiang Province, China.

Laparoscopy is widely used because it induces minimal postoperative pain and facilitates rapid recovery. However, carbon dioxide (CO) embolism is a rare but potentially fatal complication of laparoscopic surgery. Earlier reports have shown that decreased end-tidal CO (ETCO) and increased partial pressure of CO might be useful indicators of CO embolism. We herein report a case of CO embolism after the freed bladder neck was released during laparoscopic radical prostatectomy. Sudden hemodynamic disorder and increased ETCO combined with immediate arterial blood gas analysis led us to suspect CO embolism, which was confirmed by the aspiration of foamy blood from the central venous catheter. The patient was successfully resuscitated and recovered well. This case illustrates that hemodynamic collapse accompanied by increased ETCO can indicate CO embolism.
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http://dx.doi.org/10.1177/03000605211004765DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072814PMC
April 2021

Increased Circulating T Follicular Helper Cells Induced IL-12/21 in Patients With Acute on Chronic Hepatitis B Liver Failure.

Front Immunol 2021 31;12:641362. Epub 2021 Mar 31.

Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: T Follicular helper (Tfh) cells, recognized as a distinct CD4 T cell subset, mediate the development of long-lived humoral immunity B cell activation/differentiation. Tfh cells play an important role during hepatic viral infection, but its role in hepatitis B virus-related acute on chronic liver failure (HBV-ACLF) remains to be explored.

Materials And Methods: The frequency of Tfh cells, serum pro-inflammatory cytokine (IL-12, IL-21, IL-17 and TNF) levels and IgG/M levels were investigated in HBV-ACLF (n = 36), serious chronic hepatitis B (n = 21), moderate chronic hepatitis B patients (n = 32) and healthy control (HC) subjects (n = 10).

Results: Circulating Tfh cells were significantly increased in HBV-ACLF patients compared to other groups, correlating well with MELD score. However, the frequency of Tfh cells decreased in ameliorated HBV-ACLF patients. Furthermore, serum IL-12 and IL-21 levels were higher in HBV-ACLF patients, compared to other groups. Naïve CD4 T cells from HC subjects differentiate into Tfh cells following treatment with HBV-ACLF patients' serum, a process that can be blocked by IL-12/21 neutralizing antibodies. Tfh cells induced by HBV-ACLF patient's serum promoted the proliferation and IgG production of B cells . Moreover, circulating CD19 B cells, serum and liver IgG/M levels were significantly higher in HBV-ACLF patients, compared to other groups.

Conclusions: Our data demonstrated that there was a high frequency of Tfh cells and high levels of serum IL-12/21 in HBV-ACLF patients. Naïve CD4 T cells differentiate into Tfh cells in the presence of HBV-ACLF patients' serum rich in IL-12/21, which can be blocked by neutralizing IL-12/21 antibodies. These data may provide useful insights for both clinical and basic research in the treatment of HBV-ACLF.
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http://dx.doi.org/10.3389/fimmu.2021.641362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044369PMC
March 2021

High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.

J Neuroinflammation 2021 Apr 12;18(1):90. Epub 2021 Apr 12.

Department of Neurology, Mental and Neurological Disease Research Center, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, Guangdong, 510630, People's Republic of China.

Background: A high-salt diet (HSD) is one of the major risk factors for acute ischemic stroke (AIS). As a potential mechanism, surplus salt intake primes macrophages towards a proinflammatory phenotype. In this study, whether HSD could blunt the efferocytic capability of macrophages after ischemic stroke, thus exacerbating post-stroke neural inflammation, was investigated.

Methods: Wild-type male C57BL/6 mice were fed with fodder containing 8% sodium chloride for 4 weeks and subjected to transient middle cerebral occlusion (tMCAO). Disease severity, macrophage polarization as well as efferocytic capability were evaluated. Bone marrow-derived macrophages were cultured in vitro, and the impact of high salinity on their efferocytic activity, as well as their expression of phagocytic molecules, were analyzed. The relationships among sodium concentration, macrophage phenotype, and disease severity in AIS patients were explored.

Results: HSD-fed mice displayed increased infarct volume and aggravated neurological deficiency. Mice fed with HSD suffered exacerbated neural inflammation as shown by higher inflammatory mediator expression and immune cell infiltration levels. Infiltrated macrophages within stroke lesions in HSD-fed mice exhibited a shift towards proinflammatory phenotype and impaired efferocytic capability. As assessed with a PCR array, the expression of triggering receptor expressed on myeloid cells 2 (TREM2), a receptor relevant to phagocytosis, was downregulated in high-salt-treated bone marrow-derived macrophages. Enhancement of TREM2 signaling restored the efferocytic capacity and cellular inflammation resolution of macrophages in a high salinity environment in vitro and in vivo. A high concentration of urine sodium in AIS patients was found to be correlated with lower TREM2 expression and detrimental stroke outcomes.

Conclusions: HSD inhibited the efferocytic capacity of macrophages by downregulating TREM2 expression, thus impeding inflammation resolution after ischemic stroke. Enhancing TREM2 signaling in monocytes/macrophages could be a promising therapeutic strategy to enhance efferocytosis and promote post-stroke inflammation resolution.
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http://dx.doi.org/10.1186/s12974-021-02144-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040220PMC
April 2021

The Association of Bisphenol A and Phthalates with Risk of Breast Cancer: A Meta-Analysis.

Int J Environ Res Public Health 2021 03 1;18(5). Epub 2021 Mar 1.

Department of Occupational and Environmental Health, School of Health Sciences, Wuhan University, Wuhan 430071, China.

Background: Breast cancer is the most common cancer and the second leading cause of cancer-related death amongst American women. Endocrine-disrupting chemicals (EDCs), especially bisphenol A (BPA) and phthalates, have adverse effects on human health. However, the association of BPA and phthalates with breast cancer remains conflicting. This study aims to investigate the association of BPA and phthalates with breast cancer.

Methods: Correlative studies were identified by systematically searching three electronic databases, namely, PubMed, Web of Sciences, and Embase, up to November 2020. All data were analyzed using Stata 15.0.

Results: A total of nine studies, consisting of 7820 breast cancer cases and controls, were included. The urinary phthalate metabolite mono-benzyl phthalate (MBzP) and mono-2-isobutyl phthalate (MiBP) were negatively associated with breast cancer (OR = 0.73, 95% CI: 0.60-0.90; OR = 0.75, 95% CI: 0.58-0.98, respectively). However, the overall ORs for BPA, mono-ethyl phthalate (MEP), mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono-2-ethylhexyl phthalate (MEHP), mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP), mono-(3-carboxypropyl) phthalate (MCPP), and mono-butyl phthalate (MBP) were 0.85 (95% CI: 0.69-1.05), 0.96 (95% CI: 0.62-1.48), 1.12 (95% CI: 0.88-1.42), 1.13 (95% CI: 0.74-1.73), 1.01 (95% CI: 0.74-1.40), 0.74 (95% CI: 0.48-1.14), and 0.80 (95% CI: 0.55-1.15), respectively, suggesting no significant association. The sensitivity analysis indicated that the results were relatively stable.

Conclusion: Phthalate metabolites MBzP and MiBP were passively associated with breast cancer, whereas no associations were found between BPA, MEP, MEHHP, MEHP, MEOHP, MCPP, and MBP and breast cancer. More high-quality case-control studies or persuasive cohort studies are urgently needed to draw the best conclusions.
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http://dx.doi.org/10.3390/ijerph18052375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967730PMC
March 2021

The farnesoid X receptor agonist tropifexor prevents liver damage in parenteral nutrition-fed neonatal piglets.

J Pediatr Gastroenterol Nutr 2021 Mar 26. Epub 2021 Mar 26.

Department of Pediatric Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China Department of Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China Shanghai Institute for Pediatric Research, Shanghai, China Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China.

Objectives: Intestinal failure-associated liver disease (IFALD) is a life-threatening complication for patients with intestinal failure (IF) who receive long-term parenteral nutrition (PN). We evaluated the effects of the farnesoid X receptor (FXR) agonist tropifexor on a neonatal piglet model of IFALD fed with PN.

Methods: The piglets received PN and tropifexor for 14 days, then levels of liver enzymes, bile acid metabolism, inflammation, and intestinal barrier markers were assessed using quantitative real-time PCR (qRT-PCR). Fibroblast growth factor (FGF) 19 serum levels were determined using enzyme-linked immunosorbent assays (ELISA). Bile acids were determined in liver, serum, and intestinal contents, and the microbiome was sequenced in different intestinal segments.

Results: The PN model was established in newborn piglets. The levels of serum liver enzymes, pro-inflammatory factors, and oxidative stress increased in the livers of piglets fed with PN, but not in those fed with PN and tropifexor. Tropifexor stimulated FGF19 expression in ileal epithelial cells, increased portal FGF19 levels, then inhibited cholesterol 7α-hydroxylase (CYP7A1) expression in the liver. Tropifexor increased the relative abundance of bacteria associated with bile salt hydrolase and 7α-dehydrogenation in the contents of ileum and altered the composition of bile acids in serum, liver, and intestinal contents. Tropifexor also inhibited intestinal inflammation, alleviated intestinal mucosal atrophy, and improved the intestinal barrier.

Conclusions: Tropifexor might prevent liver damage in neonatal piglets receiving PN by altering the composition of intestinal microbiota and bile acids. Tropifexor also alleviates intestinal inflammation and preserves the intestinal barrier.
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http://dx.doi.org/10.1097/MPG.0000000000003135DOI Listing
March 2021

Protective effects of total flavonoids from Qu Zhi Qiao (fruit of Citrus paradisi cv. Changshanhuyou) on OVA-induced allergic airway inflammation and remodeling through MAPKs and Smad2/3 signaling pathway.

Biomed Pharmacother 2021 Jun 19;138:111421. Epub 2021 Mar 19.

Zhejiang You-du Biotech Limited Company, Quzhou 324200, China; Department of Pharmacy, School of Medicine, Zhejiang University City College, 310015 China. Electronic address:

Allergic asthma is one of the inflammatory diseases, which has become a major public health problem. Qu zhi qiao (QZQ), a dry and immature fruit of Citrus paradisi cv. Changshanhuyou, has various flavonoids with pharmacological properties. However, there is a knowledge gap on the pharmacological properties of QZQ on allergic asthma. Therefore, here, we explored the efficacy and mechanism of total flavonoids from QZQ (TFCH) on allergic asthma. We extracted and purified TFCH and conducted animal experiments using an Ovalbumin (OVA)-induced mice model. Bronchoalveolar lavage fluid and Swiss-Giemsa staining were used to count different inflammatory cells in allergic asthma mice. We conducted histopathology and immunohistochemistry to evaluate the changes in the lungs of allergic asthma mice. Moreover, we used ELISA assays to analyze chemokines and inflammatory cytokines. Furthermore, western blot analyses were conducted to elucidate the mechanism of TFCH on allergic asthma. We established that TFCH has anti-inflammatory effects and inhibits airway remodeling, providing a potential therapeutic strategy for allergic asthma.
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http://dx.doi.org/10.1016/j.biopha.2021.111421DOI Listing
June 2021

Nutrition profile of very low birth weight infants with extrauterine growth restriction in NICU.

Clin Nutr ESPEN 2021 Apr 9;42:252-257. Epub 2021 Feb 9.

Department of Clinical Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China; Department of Pediatric Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background & Aims: Extrauterine growth restriction (EUGR) is associated with long-term complications such as neurodevelopmental dysplasia, increased mortality, and chronic metabolic disease. The incidence of EUGR in very low birth weight infants (VLBWIs) is generally high. This study's objectives were to (1) evaluate the nutritional support of VLBWIs with EUGR in our hospital NICU in the past 2 y and (2) provide guidance for improving clinical practice.

Methods: Preterm infants (birth weight < 1500 g) admitted to our hospital from February 2017 to July 2019 were enrolled in the study. Nutrient intakes were recorded daily, and growth parameters were regularly measured. Based on whether the infants reached the 10th percentile of the 2013 Fenton growth curve at discharge, the infants were divided into a EUGR group (n = 134) and a non-EUGR group (n = 34) and their nutrition support were compared with current ESPGHAN guidelines.

Results: A total of 138 VLBWIs were enrolled in the study. Growth restriction was 18.1% at birth and 75.4% at discharge for weight. Enteral nutrition (EN) was initiated late compared with the guidelines. The cumulative EN interruption time was long, especially in the EUGR group. Insufficient energy and amino acid intakes were prevalent, and cumulative energy and amino acid deficits failed to be compensated at discharge. Lower Z-score at birth (OR = 0.055, 95% CI = 0.018-0.172, p < 0.001) and long cumulative interruption time (OR = 1.058, 95% CI = 1.001-1.119, p = 0.046) were risk factors for EUGR incidence.

Conclusion: In general, the nutritional support for VLBWIs was inadequate, conservative enteral feeding was the main reason.
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http://dx.doi.org/10.1016/j.clnesp.2021.01.027DOI Listing
April 2021

MiR-129-5p Suppresses Cell Proliferation of Human Osteosarcoma Cancer by Down-Regulating LncRNA Lnc712.

Cancer Manag Res 2021 10;13:2259-2264. Epub 2021 Mar 10.

Department of Orthopaedics, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an City, Nanjing Province, 223300, People's Republic of China.

Introduction: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).

Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay.

Results: Lnc712 was upregulated in OS and was inversely correlated with miR-129-5p. In OS cells, Lnc712 overexpression failed to significantly affect miR-129-5p, while miR-129-5p overexpression led to downregulated Lnc712. Cell proliferation showed that Lnc712 overexpression resulted in increased cell proliferation rate. MiR-129-5p overexpression played an opposite role and reversed the effect of Lnc712 overexpression.

Discussion: MiR-129-5p may suppress cell proliferation of OS by down-regulating Lnc712.
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http://dx.doi.org/10.2147/CMAR.S284078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956586PMC
March 2021

The Difference Spotting Task: A new nonverbal measure of cheating behavior.

Behav Res Methods 2021 Mar 10. Epub 2021 Mar 10.

School of Psychology, Shenzhen University, Shenzhen, 518060, China.

To understand when, how, and why people cheat, the ability to detect cheating in a laboratory setting is crucial. However, commonly used paradigms are confronted with a conflict between allowing participants to believe they can cheat unnoticed and allowing experimenters to detect cheating. This project aimed to develop and establish a new nonverbal task to resolve this conflict. Study 1 and Study 2 developed a new unsolvable paradigm called the Difference Spotting Task. In Study 1, participants were incentivized to indicate whether they found any difference between a pair of pictures without being asked to point the difference(s) out, so they could overreport their performance to earn extra money. Unbeknownst to them, the pairs of pictures from half of the items were identical so that the task could not be solved without cheating. This paradigm allowed experimenters to detect cheating for each unsolvable item. Study 3 examined the validity of the Difference Spotting Task and demonstrated it as a valid tool to assess cheating. The Difference Spotting Task is nonverbal and thus applicable to populations across age, educational level, and culture. In this unsolvable task, participants feel safe in cheating, and experimenters can detect cheating at the item level. The task holds the potential to gain acceptance by many researchers and facilitate the investigation of the underlying processes of cheating behavior.
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http://dx.doi.org/10.3758/s13428-020-01526-wDOI Listing
March 2021

Add-On Chinese Medicine for Coronavirus Disease 2019 (ACCORD): A Retrospective Cohort Study of Hospital Registries.

Am J Chin Med 2021 5;49(3):543-575. Epub 2021 Mar 5.

Hepatic Disease Institute, Hubei Key Laboratory of Theoretical and Applied, Research of Liver and Kidney in Traditional Chinese Medicine, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, P. R. China.

Chinese medicine (CM) was extensively used to treat COVID-19 in China. We aimed to evaluate the real-world effectiveness of add-on semi-individualized CM during the outbreak. A retrospective cohort of 1788 adult confirmed COVID-19 patients were recruited from 2235 consecutive linked records retrieved from five hospitals in Wuhan during 15 January to 13 March 2020. The mortality of add-on semi-individualized CM users and non-users was compared by inverse probability weighted hazard ratio (HR) and by propensity score matching. Change of biomarkers was compared between groups, and the frequency of CMs used was analyzed. Subgroup analysis was performed to stratify disease severity and dose of CM exposure. The crude mortality was 3.8% in the semi-individualized CM user group and 17.0% among the non-users. Add-on CM was associated with a mortality reduction of 58% (HR = 0.42, 95% CI: 0.23 to 0.77, [Formula: see text] = 0.005) among all COVID-19 cases and 66% (HR = 0.34, 95% CI: 0.15 to 0.76, [Formula: see text] = 0.009) among severe/critical COVID-19 cases demonstrating dose-dependent response, after inversely weighted with propensity score. The result was robust in various stratified, weighted, matched, adjusted and sensitivity analyses. Severe/critical patients that received add-on CM had a trend of stabilized D-dimer level after 3-7 days of admission when compared to baseline. Immunomodulating and anti-asthmatic CMs were most used. Add-on semi-individualized CM was associated with significantly reduced mortality, especially among severe/critical cases. Chinese medicine could be considered as an add-on regimen for trial use.
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http://dx.doi.org/10.1142/S0192415X21500257DOI Listing
April 2021

Blockage of NLRP3 inflammasome activation ameliorates acute inflammatory injury and long-term cognitive impairment induced by necrotizing enterocolitis in mice.

J Neuroinflammation 2021 Mar 6;18(1):66. Epub 2021 Mar 6.

Department of Pediatric Surgery, Xinhua hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Background: Necrotizing enterocolitis (NEC) is an inflammatory gastrointestinal disease in premature neonates with high mortality and morbidity, while the underlining mechanism of intestinal injury and profound neurological dysfunction remains unclear. Here, we aimed to investigate the involvement of NLPR3 inflammasome activation in NEC-related enterocolitis and neuroinflammation, especially long-term cognitive impairment, meanwhile, explore the protective effect of NLRP3 inhibitor MCC950 on NEC in mice.

Methods: NLRP3 inflammasome activation in the intestine and brain was assessed in the NEC mouse model, and NLRP3 inhibitor MCC950 was administrated during the development of NEC. Survival rate, histopathological injury of the intestine and brain, and expression of mature IL-1β and other pro-inflammatory cytokines were analyzed. Long-term cognitive impairment was evaluated by behavioral test.

Results: The expression of NLRP3 and mature IL-1β in the intestine and brain was greatly upregulated in NEC mice compared to the controls. MCC950 treatment efficiently improved NEC survival rate, reduced intestinal and brain inflammation, and ameliorated the severity of pathological damage in both organs. Additionally, in vivo blockage of NLRP3 inflammasome with MCC950 in early life of NEC pups potently protected against NEC-associated long-term cognitive impairment.

Conclusions: Our findings suggest that NLRP3 inflammasome activation participates in NEC-induced intestinal and brain injury, and early intervention with NLRP3 inhibitor may provide beneficial therapeutic effect on NEC infants.
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http://dx.doi.org/10.1186/s12974-021-02111-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937302PMC
March 2021

Ouabain Exhibited Strong Anticancer Effects in Melanoma Cells via Induction of Apoptosis, G2/M Phase Arrest, and Migration Inhibition.

Onco Targets Ther 2021 25;14:1261-1273. Epub 2021 Feb 25.

Burn and Plastic Surgery, Zhongda Hospital Affiliated Southeast University, Nanjing, 210009, People's Republic of China.

Background: Malignant melanoma was characterized by insensitive chemotherapy, drug resistance, and high metastatic ability, which resulted in the main reason for the mortality among skin-related cancers. The current agents were not sufficient to improve the treatment status of melanoma patients, and it was still needed to develop new chemotherapeutic drugs for melanoma. Our study aimed to study the anticancer effects and potential mechanisms of ouabain on melanoma cells.

Methods: The inhibitory effects of ouabain were determined by CCK8 and colony formation assays, and the morphological changes of melanoma cells were observed by inverted microscope. The apoptosis induction and cell cycle distribution were detected by annexin V/PI double staining and PI staining, respectively. The expression of the biomarker proteins in apoptosis and G2/M phase were determined by Western blotting analysis. The effects of ouabain on the migration of melanoma cells were measured by transwell migration assay and wound closure analysis. The potential mechanisms of ouabain in melanoma cells were analyzed by transcriptome sequencing.

Results: Our present study demonstrated that ouabain exhibited strong inhibitory effects on cell proliferation and triggered dramatical morphological changes of melanoma cells. Moreover, ouabain induced significant apoptosis in A375 rather than SK-Mel-28 cells via upregulation of bax expression and downregulation of bcl-2 expression. Consistently, ouabain treatment induced cell cycle arrest at G2/M phase in both A375 and SK-Mel-28 cells via upregulation of cyclin B1 and downregulation of cdc2 and cdc25c. Importantly, ouabain suppressed the migration of A375 and SK-Mel-28 cells. Furthermore, the transcriptome sequencing demonstrated that p53 and MAPK signaling pathway might play important roles in the inhibitory effects of ouabain.

Conclusion: Our study revealed that ouabain exhibited dramatical anticancer effects, which provided a novel application for cardiac glycoside drugs in the clinical treatment of melanoma.
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http://dx.doi.org/10.2147/OTT.S283548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920615PMC
February 2021

Parameter redundancy in Jolly-Seber tag loss models.

Ecol Evol 2021 Feb 13;11(3):1131-1149. Epub 2021 Jan 13.

Department of Mathematics and Statistics University of Victoria Victoria British Columbia Canada.

Capture-recapture experiments are conducted to estimate population parameters such as population size, survival rates, and capture rates. Typically, individuals are captured and given unique tags, then recaptured over several time periods with the assumption that these tags are not lost. However, for some populations, tag loss cannot be assumed negligible. The Jolly-Seber tag loss model is used when the no-tag-loss assumption is invalid. Further, the model has been extended to incorporate group heterogeneity, which allows parameters to vary by group membership. Many mark-recapture models become overparameterized resulting in the inability to independently estimate parameters. This is known as parameter redundancy.We investigate parameter redundancy using symbolic methods. Because of the complex structure of some tag loss models, the methods cannot always be applied directly. Instead, we develop a simple combination of parameters that can be used to investigate parameter redundancy in tag loss models.The incorporation of tag loss and group heterogeneity into Jolly-Seber models does not result in further parameter redundancies. Furthermore, using hybrid methods we studied the parameter redundancy caused by data through case studies and generated tag histories with different parameter values.Smaller capture and survival rates are found to cause parameter redundancy in these models. These problems resolve when applied to large populations.
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http://dx.doi.org/10.1002/ece3.7035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863437PMC
February 2021

Rapid Identification and Systematic Mechanism of Flavonoids from Bornm. Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Network Pharmacology.

Int J Anal Chem 2021 27;2021:6619959. Epub 2021 Jan 27.

Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 41800, China.

Bornm. (P. freyniana), belonging to the family Rosaceae, has been used as a folk medicine in China. However, as we know, the constituents and the systematic elucidation of the mechanism were not fully investigated. Therefore, it is necessary to develop a rapid method using LC-MS and network pharmacology for the detection and identification of constituents and the systematic mechanism of . Firstly, the flavonoids were detected and identified based on ultra-high-performance liquid chromatography coupled with Quadrupole-Exactive Focus Orbitrap MS (UHPLC-Q-Exactive Orbitrap MS). After that, the potential targets of those constituents were obtained by database mining. Then, the core targets were predicted by protein-protein interaction network and network analysis. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out via DAVID. This finding revealed that possessed 43 flavonoids (40 of them were first reported) with 23 core target genes, which are associated with PI3K-Akt, MAPK, TNF signaling pathway, and pathway in cancer. This study demonstrated the multicompound, multitarget, and multimechanism of , which are very beneficial to develop the further study and utilization of this plant including the material basis and quality control research.
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http://dx.doi.org/10.1155/2021/6619959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857931PMC
January 2021

Pulmonary Artery Catheter in Patients with Severe Acute Pancreatitis: A Single-Center Retrospective Study.

Dig Dis Sci 2021 Feb 11. Epub 2021 Feb 11.

Department of Gastroenterology and Hepatology, West China Hospital, Sichuan University, No.37 Guoxue Alley, Wuhou District, Chengdu, 610041, Sichuan, China.

Objective: It is still uncertain what effects pulmonary artery catheter (PAC)-guided resuscitation has on outcomes for patients with severe acute pancreatitis (SAP). Therefore, we aimed to investigate the effect of PAC on hospital mortality in patients with SAP.

Methods: We collected the data of patients with a diagnosis of SAP from January 10, 2017, to July 30, 2019. Patients were divided into a PAC group and a control group. The primary outcome measured was the day-28 mortality. Secondary outcomes included day-90 mortality, duration of ICU and hospital stay, ventilation days, usage of renal support and vasoactive agents, incidences of acute abdominal compartment syndrome, infusion volumes, and fluid balance and hemodynamic characteristics measured by the PAC. Kaplan-Meier analysis was applied to estimate survival outcomes. Complications related to PAC were also analyzed.

Results: There was no significant difference between the PAC group and the control group for day-28 mortality (22.7% vs. 30%, odds ratio, 0.69; 95% CI 0.31-1.52; P = 0.35). The duration of ICU stay in the PAC group was shorter (P = 0.00), and the rate of dependence on renal support treatment was lower in the PAC group than in the control group (P = 0.03). There was no difference in other secondary outcomes and no significant difference in the survival curve between the two groups (log-rank P = 0.72, X = 0.13). However, SAP patients inserted PAC within 24 h ICU admission showed that duration of renal support therapy in PAC patients within 24 h ICU admission (mean days, 1.60; standard deviation, 0.14) was shorter than those with 24-72 h ICU admission (mean days, 2.94; standard deviation, 0.73; P = 0.03). The organ failure rates (1 organ, 2 organs and 3 organs) were all lower in PAC patients within 24 h ICU admission than with 24-72 h ICU admission (P = 0.02, P = 0.02, P = 0.048, respectively).

Conclusion: In patients with severe acute pancreatitis, PAC-guided fluid resuscitation shortened the duration of ICU stay, and patients in the PAC group had a lower rate of dependence on renal support, while no benefit in terms of mortality was observed. However, SAP patients inserted PAC within 24 h ICU admission showed shorter duration of renal support therapy and lower organ failure rates than those with 24-72 h ICU admission, indicating that early use of PAC, especially within 24 h, might be better for SAP patients.
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http://dx.doi.org/10.1007/s10620-021-06881-yDOI Listing
February 2021