Publications by authors named "Wei Cai"

1,050 Publications

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Lignans from Ameliorated Influenza A Virus-Induced Pneumonia via Inhibiting Macrophage Activation.

Evid Based Complement Alternat Med 2022 30;2022:1688826. Epub 2022 Jul 30.

Zhejiang Chinese Medical University, Hangzhou 310053, China.

The lower respiratory tract infection, induced by influenza virus, coronaviruses, and respiratory syncytial virus, remains a serious threat to human health that can cause a global pandemic. Thus, finding effective chemicals and therapeutic measures to advance the functional restoration of the respiratory tract after infection has been the emphasis of the studies on the subjects. is a natural medicinal plant used for treating various lung and gastrointestinal diseases, including viral infection, cough, chronic obstructive pulmonary disease, acute gastroenteritis, and diarrhoea. In this study, the antiviral and anti-inflammatory effects of total lignans (MSTL) extracted from the plant were investigated in influenza A virus (IAV)-infected mice and RAW 264.7 macrophages. MSTL could not only protect the macrophages against IAV-induced pyroptosis but also could lighten the lung inflammation induced by IAV in vivo and in vitro. The network pharmacology analysis revealed that differentially expressed genes, mainly involving in EGFR tyrosine kinase inhibitor resistance, endocrine resistance, HIF-1 signaling pathway, C-type lectin receptor signaling pathway, and FOXO signaling pathway, contributed to the IAV-induced alveolar macrophage dysfunction. It indicated that MSTL enhanced the function of alveolar macrophages and improved IAV-induced lung injury in mice.
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http://dx.doi.org/10.1155/2022/1688826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356792PMC
July 2022

MLEE: A method for extracting object-level medical knowledge graph entities from Chinese clinical records.

Front Genet 2022 22;13:900242. Epub 2022 Jul 22.

School of Computer Science and Engineering Northeastern University, Shenyang, China.

As a typical knowledge-intensive industry, the medical field uses knowledge graph technology to construct causal inference calculations, such as "symptom-disease", "laboratory examination/imaging examination-disease", and "disease-treatment method". The continuous expansion of large electronic clinical records provides an opportunity to learn medical knowledge by machine learning. In this process, how to extract entities with a medical logic structure and how to make entity extraction more consistent with the logic of the text content in electronic clinical records are two issues that have become key in building a high-quality, medical knowledge graph. In this work, we describe a method for extracting medical entities using real Chinese clinical electronic clinical records. We define a computational architecture named MLEE to extract object-level entities with "object-attribute" dependencies. We conducted experiments based on randomly selected electronic clinical records of 1,000 patients from Shengjing Hospital of China Medical University to verify the effectiveness of the method.
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http://dx.doi.org/10.3389/fgene.2022.900242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354090PMC
July 2022

Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.

J Clin Invest 2022 Aug;132(15)

Pittsburgh Institute of Brain Disorders and Recovery, and Department of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Immunomodulation holds therapeutic promise against brain injuries, but leveraging this approach requires a precise understanding of mechanisms. We report that CD8+CD122+CD49dlo T regulatory-like cells (CD8+ TRLs) are among the earliest lymphocytes to infiltrate mouse brains after ischemic stroke and temper inflammation; they also confer neuroprotection. TRL depletion worsened stroke outcomes, an effect reversed by CD8+ TRL reconstitution. The CXCR3/CXCL10 axis served as the brain-homing mechanism for CD8+ TRLs. Upon brain entry, CD8+ TRLs were reprogrammed to upregulate leukemia inhibitory factor (LIF) receptor, epidermal growth factor-like transforming growth factor (ETGF), and interleukin 10 (IL-10). LIF/LIF receptor interactions induced ETGF and IL-10 production in CD8+ TRLs. While IL-10 induction was important for the antiinflammatory effects of CD8+ TRLs, ETGF provided direct neuroprotection. Poststroke intravenous transfer of CD8+ TRLs reduced infarction, promoting long-term neurological recovery in young males or aged mice of both sexes. Thus, these unique CD8+ TRLs serve as early responders to rally defenses against stroke, offering fresh perspectives for clinical translation.
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http://dx.doi.org/10.1172/JCI157678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337834PMC
August 2022

Association Between Serum Albumin Level and Microvascular Complications of Type 2 Diabetes Mellitus.

Diabetes Metab Syndr Obes 2022 23;15:2173-2182. Epub 2022 Jul 23.

Department of Endocrinology and Metabolism, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.

Objective: To analyze the associations between serum albumin (sALB) level and diabetic microvascular complications, including diabetic retinopathy (DR) and diabetic kidney disease (DKD), in patients with type 2 diabetes mellitus (T2DM).

Methods: This retrospective study included 951 hospitalized patients with T2DM who had completed screening for DR and DKD during hospitalization. Patients were divided into three groups according to sALB tertiles. Multivariate logistic regression analysis was used to assess the association of sALB with microvascular complications.

Results: The prevalence of DR, DKD and macroalbuminuria increased with decreasing sALB levels. Multivariate logistic regression analysis showed that lower levels of sALB (Q1) were associated with higher risk of DR (odds ratio [OR]: 1.59, 95% confidence interval [CI]: 1.12-2.26), DKD (OR: 3.00, 95% CI: 2.04-4.41) and macroalbuminuria (OR: 9.76, 95% CI: 4.62-20.63) compared with higher levels of sALB (Q3) after adjustment for other risk factors. After stratification by sex and age, the effect of lower levels of sALB (Q1) on DR incidence was more obvious in patients with male (OR: 1.60, 95% CI: 1.00-2.56), and aged<65 years (OR: 1.74, 95% CI: 1.14-2.65) ( < 0.05 for all); the effect of lower levels of sALB (Q1) on the incidence of DKD was significant in both males (OR: 3.78, 95% CI: 2.26-6.32) and females (OR: 2.35, 95% CI: 1.26-4.35) ( < 0.05 for all), while only the age <65 years (OR: 3.46, 95% CI: 2.16-5.53) was significant in the age subgroup ( < 0.001).

Conclusion: Decreased sALB levels may be an independent risk indicator of DR and DKD in patients with T2DM, and significantly associated with DKD progression. For DR screening, special attention should be paid to men aged <65 years, while screening for DKD should pay attention to people <65 years old.
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http://dx.doi.org/10.2147/DMSO.S373160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329574PMC
July 2022

Identification and Quantification of Chlorogenic Acids from the Root Bark of by UHPLC-Q-Exactive Orbitrap Mass Spectrometry.

ACS Omega 2022 Jul 11;7(29):25675-25685. Epub 2022 Jul 11.

School of Pharmaceutical Sciences & Hunan University of Medicine, Huaihua 418000, P.R. China.

The purpose of this study is to identify and quantify the chlorogenic acids (CGAs) from the root bark of , which is conventionally regarded as a tonic in folk Chinese Traditional medicine. The effective methods for identification and quantification analysis of CGAs were developed based on ultra high performance liquid chromatography-Q-exactive orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) in parallel reaction monitoring (PRM) and selected reaction monitoring (SIM), which showed high sensitivity and resolution for screening and quantifying compounds. The root bark of was extracted under ultrasonication with 70% methanol. Ultimately, a for total of 70 CGAs, 64 of these were tentatively identified for the first time. Moreover, a methodological study of seven kinds of CGAs was carried out. The proposed procedure was optimized and validated in terms of selectivity, linearity of analytical curves ( > 0.990), accuracy (recovery range from 96.7 to 105%), and repeatability (relative standard deviation <5%). Then it was applied to determine the content of the CGAs in roots from 66 of different batches. The total CGAs was quantified in a range between 2.150 and 33.51 mg/g, which could be considered as excellent source of natural bioactive compound. The result was extremely useful for understanding the bioactive substance and quality control of in depth.
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http://dx.doi.org/10.1021/acsomega.2c02899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330223PMC
July 2022

A novel approach simultaneously imparting well-hydrophobicity and photothermal conversion effect to polymer materials: solar-promoted absorption of organic solvents and oils.

J Hazard Mater 2022 Sep 22;437:129446. Epub 2022 Jun 22.

State Key Laboratory of Fire Science, University of Science and Technology of China, Hefei 230026, PR China. Electronic address:

In this work, a series of polymer materials including pomelo peel, cotton fabric, polyurethane foam, and so on, are treated by heated CHSiCl, presenting desirable photo-thermal conversion function and hydrophobicity. As a representative material, the surface element and skeleton morphology of pomelo peel foam treated by CHSiCl are analyzed detailedly. It is found that well-hydrophobicity (water contact angle of ~147°) and photo-thermal conversion performance (~91.2 °C under one sun) are attributed to the surface carbonization reaction and formation of CH-SiO nanoparticles. Meanwhile, the treatment of CHSiCl significantly increases the BET surface area to 3.0635 m²/g from 0.0973 m²/g. Therefore, pomelo peel-derived carbon foam presents a desirable adsorption capacity of organic solvents and oils (up to 43.2 times its original weight) and excellent removal efficiency (>99.0%). In addition, the rapid photo-thermal response (achieve ~73 °C at 40 s) and high equilibrium temperature (~91.2 °C) are als° demonstrated in pomelo peel-derived carbon foam. As a result, the absorption rate of highly-viscous oils is effectively promoted by the higher fluidity and capillary action caused by the solar-promoted mechanism. This study offers a scalable, easily operated, and environmentally friendly approach to prepare hydrophobic and photo-thermal materials, thus demonstrating a huge potential in oil/water separation application.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129446DOI Listing
September 2022

Untargeted Metabolomics Reveal Parenteral Nutrition-Associated Alterations in Pediatric Patients with Short Bowel Syndrome.

Metabolites 2022 Jun 27;12(7). Epub 2022 Jun 27.

Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.

Short bowel syndrome (SBS) is a major cause of intestinal failure (IF) that may require long-term parenteral nutrition (PN) support. However, long-term PN is accompanied by severe complications such as catheter-related blood stream infection (CRBSI) and intestinal failure-associated liver disease (IFALD), and it is associated with high healthcare costs. In this study, we characterized the plasma metabolomic profile and investigated the role of metabolism in predicting long-term PN in pediatric patients with SBS. Untargeted metabolomics was performed in plasma samples from 20 SBS patients with PN support: 6 patients had IFALD and 14 patients had no liver disease. As controls, 18 subjects without liver or intestinal diseases were included for the analysis. SBS patients had distinct plasma metabolomic signatures compared to controls, and several pathways associated with amino acid metabolism and cell death were significantly changed. The presence of IFALD in SBS was associated with alterations of metabolites mainly classified as "amino acids, peptides, and analogues" and "benzene and derivatives". Serum direct bilirubin levels were negatively correlated with levels of uridine, skatole, and glabrol. Importantly, SBS patients with long-term PN showed significantly increased levels of glutamine compared to those in the short-term PN group. Finally, using multivariate logistic regression analysis, we developed a prediction model including glutamine and creatinine to identify pediatric SBS patients who need long-term PN support. These findings underscore the potential key role of the metabolome in SBS with IF and suggest that metabolomic profiles could be used in long-term PN assessment.
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http://dx.doi.org/10.3390/metabo12070600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319335PMC
June 2022

The safety and efficacy of EUS-GE for gastric outlet obstruction in different sites: a single-center retrospective study.

J Dig Dis 2022 Jul 26. Epub 2022 Jul 26.

Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School.

Background: Gastric outlet obstruction (GOO) is a common indication for palliative surgical consultation. Recently, EUS-guided gastroenterostomy (EUS-GE) has become a novel option. The aim of this study is to compare the safety and efficacy of EUS-GE in different obstruction positions.

Methods: Consecutive patients, who underwent EUS-GE for GOO from March 2017 to April 2020 in our center and stayed in gastroenterology ward after the operation, were involved in this retrospective study. Patients were divided into three groups depending on obstruction site. The primary outcomes included technical success and clinical success. The secondary outcomes were operation time, post-procedure length of stay (LOS), hospitalization costs and complications, including peritonitis, bleeding, pneumoperitoneum, abdominal pain and infection.

Results: A total of 51 patients were included. Technical success rate was 100% in the proximal position, while 88.9% in the distal position (P = 0.176). Clinical success declined from the oral side to the anal side (P = 0.510). Mean operation time, hospitalization costs and post-operation LOS were similar (P = 0.532, 0.520, and 0.144, respectively). Complications were observed in 28 patients (54.9%). When reaching mature phase, clinical success rate enhanced and the secondary outcomes showed no significant difference.

Conclusions: EUS-GE may be challenging for distal GOO, however it was safe and effective to be conducted by experienced endoscopists. Taking complete preoperative evaluation to evaluate the difficulty degree is necessary. Larger and prospective data are needed to further validate our finding.
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http://dx.doi.org/10.1111/1751-2980.13118DOI Listing
July 2022

The relationship between high-normal blood pressure in the first half of pregnancy and the risk of hypertensive disease of pregnancy.

J Clin Hypertens (Greenwich) 2022 Jul 20. Epub 2022 Jul 20.

Department of Cardiology, Tianjin Economic-Technological Development Area (TEDA) International Cardiovascular Hospital, Tianjin, R.P. China.

Early warning of hypertensive disorder in pregnancy (HDP) can improve maternal and infant outcomes. However, few studies had evaluated the warning value of high-normal blood pressure (BP) before the onset of HDP. This was a prospective cohort study to investigate the relationship between high-normal BP in the first half of pregnancy and the risk of HDP. According to the maximum BP measured before 20  weeks of gestation, the cohort was divided into three groups: optimal BP (SBP < 120 mmHg and DBP < 80 mmHg), normal BP (120 mmHg ≤ SBP < 130 mmHg or 80 mmHg ≤ DBP < 85 mmHg), and high-normal BP (130 mmHg ≤ SBP < 140 mmHg or 85 mmHg ≤ DBP < 90 mmHg). The relationship between different BP levels in the first half of pregnancy and HDP risk was assessed by general linear models. Ten thousand one hundred and ninety-three normotensive pregnant women with complete information were finally included for data analysis. Among them, 532 pregnant women were diagnosed with HDP, with a total HDP incidence of 5.2%. The incidences in the optimal, normal, and high-normal BP groups were 2.4%, 6.0%, and 21.8%, respectively. Compared to women with optimal BP in the first half of pregnancy, women with high-normal BP had a 445% increased risk of HDP (aRR: 5.45, 95% CI: 4.24-7.00), and even women with normal BP had a 107% increased risk of HDP (aRR: 2.07, 95% CI: 1.68-2.56). This study demonstrated that among low-risk healthy women, women with high-normal BP in the first half of pregnancy had a significantly higher risk of HDP.
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http://dx.doi.org/10.1111/jch.14551DOI Listing
July 2022

Gastric cancer cell-derived extracellular vesicles disrupt endothelial integrity and promote metastasis.

Cancer Lett 2022 Jul 14;545:215827. Epub 2022 Jul 14.

Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Institute of Integrated Traditional Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address:

The endothelium is the critical barrier that controls transendothelial communications. Blood vessels in cancer tissue are poorly developed and highly permeable. However, it is poorly understood how circulating cancer cells released through these "leaky" vessels break the intact vasculature of remote organs to metastasize. We investigated the roles of cancer cell-derived extracellular vesicles (CEVs) in regulating cancer metastasis by analyzing samples from gastric cancer patients, performing in vitro experiments, and studying mouse models. We made several novel observations. First, the rate of metastasis was closely associated with plasma levels of CEVs in patients with gastric cancer. Second, cultured endothelial cells endocytosed CEVs, resulting in cytoskeletal rearrangement, low expression of the junction proteins cadherin and CD31, and forming large intercellular gaps to allow the transendothelial migration of cancer cells. The dynamin inhibitor Dynasore prevented these CEV-induced changes of endothelial cells by blocking CEVs endocytosis. Third, CEVs disrupted the endothelial barrier of cancer-bearing mice to promote cancer metastasis. Finally, lactadherin promoted the clearance of circulating CEVs to reduce metastasis. These results demonstrate the essential role of CEVs in promoting the metastasis of gastric cancer.
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http://dx.doi.org/10.1016/j.canlet.2022.215827DOI Listing
July 2022

Insertion of carbon skeleton in Ni/MoO heterojunction with porous hollow sphere for efficient alkaline electrochemical hydrogen production.

J Colloid Interface Sci 2022 Jun 4;627:21-27. Epub 2022 Jun 4.

School of Materials Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China. Electronic address:

The catalyst morphology has a strong impact on the activity of electrocatalytic hydrogen production. Considering the effect, we design and fabricate hollow spherical Ni/MoO heterojunction. In addition, an amorphous carbon skeleton is inserted into the hollow sphere, which makes the structure more stable and porous. Compared with other morphological Ni/MoO, the porous hollow spherical Ni/MoO (H-Ni/MoO) with an internal carbon skeleton shows better hydrogen evolution reaction (HER) activity with a small overpotential of 58 mV to reach 10 mA cm and a tafel value of 44.8 mV dec in alkaline media. The developed HER performance of H-Ni/MoO can be attributed to the larger active surface area of porous hollow spherical structure and the faster electron transfer and better stability of carbon skeleton. Undoubtedly, the urea plays a crucial role to construct the hollow spherical morphology and being decomposed to form holes and amorphous carbon in the synthesized steps. The soft-template strategy using urea as the addition for forming the porous hollow structure with carbon skeleton can be extended to explore superior non-noble metal for hydrogen production.
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http://dx.doi.org/10.1016/j.jcis.2022.06.001DOI Listing
June 2022

Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.

BMC Pregnancy Childbirth 2022 Jul 14;22(1):562. Epub 2022 Jul 14.

Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China.

Objective: Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns. The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants. Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs. Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance. This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.

Methods: This cross-sectional study included breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021. Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum). A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,. HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition. A total of 465 samples completed the tests in both analyzers. The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.

Results: The MIR and ultrasonic milk analyzer results were significantly different. The protein, fat, and energy determined using the MIR method were higher than those determined by the ultrasonic method, while lactose determined by the MIR method were lower (all p < 0.05). The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA. After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range. The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable. The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).

Conclusions: While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning. Machine learning methods can play a role in data fitting using different analyzers.
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http://dx.doi.org/10.1186/s12884-022-04891-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284806PMC
July 2022

Effect of high ammonia on granular stability and phosphorus recovery of algal-bacterial granules in treatment of synthetic biogas slurry.

Heliyon 2022 Jul 3;8(7):e09844. Epub 2022 Jul 3.

Hubei Provincial Key Laboratory of Regional Development and Environmental Response, Department of Environmental Engineering, Hubei University, Wuhan, 430062, China.

The aim of the study was to investigate the application of algal-bacterial granules in treatment of high ammonia wastewater. Two identical cylindrical reactors, i.e., Rc and Rs was used to develop granular sludge system with synthetic biogas slurry. Rs was run under an artificial solar lamp controlled at 12 h power on and 12 h power off (∼10,000 lux); Rc was operated as control (no light). Results showed that algal-bacterial granules (ABGS) developed in Rs exhibited better structural stability in the face of high ammonia influent. Compared with aerobic granules (AGS), ABGS possessed high proteins (PN) content (145.3 mg/g-VSS) in extracellular polymeric substances (EPS) and better O mass transfer inner granules. Higher phosphorus (P) removal capacity was obtained in Rs even under 400 mg/L NH-N which resulted in higher P content in ABGS biomass (56.4 mg/g-TSS). Bioavailable P in ABGS was 44 mg P/g-SS on day 160, approximately 1.53-times higher than that in AGS.
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http://dx.doi.org/10.1016/j.heliyon.2022.e09844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263998PMC
July 2022

Down-regulation of miR-19b-3p enhances IGF-1 expression to induce osteoblast differentiation and improve osteoporosis.

Cell Mol Biol (Noisy-le-grand) 2022 May 22;68(1):160-168. Epub 2022 May 22.

Department of Orthopaedics, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu223300, China.

This research was conducted in order to investigate the role of miR-19b-3p in the development of osteoporosis (OP) in rats and the associated mechanisms. This study measured the expression levels of miR-19b-3p and IGF-1 in clinical OP patients and ovariectomy-induced OP rats by qRT-PCR. The osteoprotegerin levels in OP patients were measured by enzyme-linked immunosorbent assay (ELISA). The binding site of miR-19b-3p to IGF-1 was predicted by three prediction sites: Target Scan, miRDB and starbase. Experiments were conducted in vitro and in vivo using bone marrow mesenchymal stem cells (BMSCs) and OP rats, respectively, to verify the regulatory relationship between miR-19b-3p and IGF-1 and explore the role of miR-19b-3p in the development of OP. Results showed that the expression of miR-19b-3p was elevated in OP patients and rats, while IGF-1 expression was decreased (***p<0.001). The ELISA assay found that osteoprotegerin levels were inversely correlated with miR-19b-3p and positively correlated with IGF-1. The predictive analysis identified binding sites for miR-19b-3p to IGF-1. The potential regulatory relationship between miR-19b-3p and IGF-1 was validated by in vitro and in vivo experiments. Moreover, the important role of miR-19b-3p in the regulation of OP was further demonstrated. It was concluded the inhibition of miR-19b-3p has a suppressive effect on the development of OP and the function of miR-19b-3p in OP is likely to be achieved by regulating the expression of the IGF-1 gene.
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http://dx.doi.org/10.14715/cmb/2022.68.1.20DOI Listing
May 2022

Using routine emergency department data for syndromic surveillance of acute respiratory illness, Germany, week 10 2017 until week 10 2021.

Euro Surveill 2022 Jul;27(27)

Robert Koch Institute, Department for Methodology and Research Infrastructure, Berlin, Germany.

BackgroundThe COVID-19 pandemic expanded the need for timely information on acute respiratory illness at population level.AimWe explored the potential of routine emergency department data for syndromic surveillance of acute respiratory illness in Germany.MethodsWe used routine attendance data from emergency departments, which continuously transferred data between week 10 2017 and 10 2021, with ICD-10 codes available for > 75% of attendances. Case definitions for acute respiratory infection (ARI), severe acute respiratory infection (SARI), influenza-like illness (ILI), respiratory syncytial virus infection (RSV) and COVID-19 were based on a combination of ICD-10 codes, and/or chief complaints, sometimes combined with information on hospitalisation and age.ResultsWe included 1,372,958 attendances from eight emergency departments. The number of attendances dropped in March 2020 during the first COVID-19 pandemic wave, increased during summer, and declined again during the resurge of COVID-19 cases in autumn and winter of 2020/21. A pattern of seasonality of respiratory infections could be observed. By using different case definitions (i.e. for ARI, SARI, ILI, RSV) both the annual influenza seasons in the years 2017-2020 and the dynamics of the COVID-19 pandemic in 2020/21 were apparent. The absence of the 2020/21 influenza season was visible, parallel to the resurge of COVID-19 cases. SARI among ARI cases peaked in April-May 2020 (17%) and November 2020-January 2021 (14%).ConclusionSyndromic surveillance using routine emergency department data can potentially be used to monitor the trends, timing, duration, magnitude and severity of illness caused by respiratory viruses, including both influenza viruses and SARS-CoV-2.
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http://dx.doi.org/10.2807/1560-7917.ES.2022.27.27.2100865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264729PMC
July 2022

TRIOL Inhibits Rapid Intracellular Acidification and Cerebral Ischemic Injury: The Role of Glutamate in Neuronal Metabolic Reprogramming.

ACS Chem Neurosci 2022 07 30;13(14):2110-2121. Epub 2022 Jun 30.

Department of Molecular Biology and Biochemistry, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.

As one of the key injury incidents, tissue acidosis in the brain occurs very quickly within several minutes upon the onset of ischemic stroke. Glutamate, an excitatory amino acid inducing neuronal excitotoxicity, has been reported to trigger the decrease in neuronal intracellular pH (pHi) via modulating proton-related membrane transporters. However, there remains a lack of clarity on the possible role of glutamate in neuronal acidosis via regulating metabolism. Here, we show that 200 μM glutamate treatment quickly promotes glycolysis and inhibits mitochondrial oxidative phosphorylation of primary cultured neurons within 15 min, leading to significant cytosolic lactate accumulation, which contributes to the rapid intracellular acidification and neuronal injury. The reprogramming of neuronal metabolism by glutamate is dependent on adenosine monophosphate-activated protein kinase (AMPK) signaling since the inhibition of AMPK activation by its selective inhibitor compound C significantly reverses these deleterious events in vitro. Moreover, 5α-androst-3β,5α,6β-TRIOL (TRIOL), a neuroprotectant we previously reported, can also remarkably reverse intracellular acidification and alleviate neuronal injury through the inhibition of AMPK signaling. Furthermore, TRIOL remarkably reduced the infarct volume and attenuated neurologic impairment in acute ischemic stroke models of middle cerebral artery occlusion in vivo. In summary, we reveal a novel role of glutamate in rapid intracellular acidification injury resulting from glutamate-induced lactate accumulation through AMPK-mediated neuronal reprogramming. Moreover, inhibition of the quick drop in neuronal pHi by TRIOL significantly reduces the cerebral damages, suggesting that it is a promising drug candidate for ischemic stroke.
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http://dx.doi.org/10.1021/acschemneuro.2c00119DOI Listing
July 2022

Cu-Based Multicomponent Metallic Compound Materials as Electrocatalyst for Water Splitting.

Front Chem 2022 13;10:913874. Epub 2022 Jun 13.

School of Materials Science and Engineering, Harbin Institute of Technology, Harbin, China.

In this study, Cu-based multicomponent metallic compound materials M-Cu (M = Mn, Fe, Co, Ni, and Pt) were studied as electrocatalytic materials for water splitting. Different metal materials attached to the copper foam substrate can change the valence states of copper and oxygen, resulting in the change of electronic structure of the materials, thus changing its catalytic activity.
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http://dx.doi.org/10.3389/fchem.2022.913874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234134PMC
June 2022

Efficient Si Doping Promoting Thermoelectric Performance of Yb-Filled CoSb-Based Skutterudites.

ACS Appl Mater Interfaces 2022 Jul 29;14(27):30901-30906. Epub 2022 Jun 29.

State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin 150001, China.

Nanocomposites have become a widely popular way to assist in the enhancement of thermoelectric performance for filled skutterudites. Herein, we unveil the distinctive effect of Si doping on the classic YbCoSb. On the one hand, the reduced Yb filling fraction is accompanied by the in-situ precipitated CoSi nanoparticles, which not only enhances the power factor in the intermediate-low temperature range but also reduces electronic thermal conductivity for decreasing the carrier concentration. On the other hand, CoSi nanoparticles intensively disrupt the phonon transport, hiding the increased lattice thermal conductivity due to reduced Yb filling fraction. Although the residual YbSb second phases have an adverse effect on the thermoelectric properties, the integration effects achieve a peak value of 1.37 at 823 K and increase by 21% for the YbCoSb/0.1Si sample.
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http://dx.doi.org/10.1021/acsami.2c07044DOI Listing
July 2022

Effects of Character Strength-Based Intervention vs Group Counseling on Post-Traumatic Growth, Well-Being, and Depression Among University Students During the COVID-19 Pandemic in Guangdong, China: A Non-Inferiority Trial.

Psychol Res Behav Manag 2022 16;15:1517-1529. Epub 2022 Jun 16.

Department of Psychology/ Research Center for Quality of Life and Applied Psychology, Guangdong Medical University, Dongguan, People's Republic of China.

Purpose: This study assessed the effects of character strengths-based invention (CSI) vs group counseling (GC) on mental health outcomes (ie, post-traumatic growth, well-being, and depression) among university students in Guangdong Province, China.

Materials And Methods: We conducted a randomized non-inferiority trial among 124 undergraduates at a medical university during the COVID-19 pandemic. We randomly allocated students to receive either CSI (n=62) or GC (n=62). A qualified psychologist delivered both activities on campus during two consecutive weekends with a total of 7.5 hours and also gave assignments to students during weekdays. We measured the outcomes at pre-intervention, post-intervention, and 1-month follow-up. We then analyzed data using descriptive statistics and a general linear mixed model.

Results: CSI and GC groups had similar baseline characteristics. Both CSI and GC experienced an increase in post-traumatic growth and well-being. The mean±SD scores for post-traumatic growth were significantly higher in CSI compared to GC groups (87.70±14.22 vs 78.15±20.72, respectively), whereas well-being scores were similar between CSI and GC (82.58±16.57 vs 83.68±15.59, respectively). Neither CSI nor GC experienced a reduction in depression scores.

Conclusion: CSI had non-inferior effects compared to GC with regards to improvement of post-traumatic growth and well-being during the COVID-19 pandemic. However, caveats regarding lack of blinding of investigator and limited generalizability should be considered in the interpretation of the study findings.
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http://dx.doi.org/10.2147/PRBM.S359073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208671PMC
June 2022

Lactobacillus plantarum supplementation alleviates liver and intestinal injury in parenteral nutrition-fed piglets.

JPEN J Parenter Enteral Nutr 2022 Jun 22. Epub 2022 Jun 22.

Division of Pediatric Gastroenterology and Nutrition, School of Medicine, Xin Hua Hospital, Shanghai Jiao Tong University, Shanghai, China.

Objective: Long-term parenteral nutrition (PN) causes PN-associated liver disease, for which therapeutic approaches are limited. This study aimed to investigate the effects of Lactobacillus plantarum CGMCC 1258 (LP) on liver and intestinal injury in PN-fed neonatal piglets.

Methods: The piglets received PN with or without oral LP for 14 days. The levels of liver enzymes and inflammatory markers were measured using biochemical kits and quantitative real-time polymerase chain reaction. Serum fibroblast growth factor 19 (FGF19) was detected using an enzyme-linked immunosorbent assay. The bile acid (BA) profiles in the liver, serum, and intestinal contents were determined using ultraperformance liquid chromatography coupled with mass spectrometry. The composition of intestinal bacteria was analyzed with 16S rRNA gene amplicon sequencing.

Results: LP supplementation was associated with improved markers of liver disease, inflammation, and oxidative stress in PN-fed piglets. Moreover, markers of intestinal injury and inflammation were alleviated by LP in PN-fed piglets. Mechanistically, LP increased the abundance of Lactobacillus in ileal contents and stimulated FGF19 expression in ileal mucosa. Subsequently, it increased the expression of small heterodimer partner (SHP) and inhibited cholesterol 7α-hydroxylase (CYP7A1) expression in the liver. Additionally, LP altered the systemic composition and metabolism of BAs.

Conclusions: LP alleviated liver and intestinal injury in PN-fed neonatal piglets by altering the composition of intestinal bacteria and BAs.
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http://dx.doi.org/10.1002/jpen.2429DOI Listing
June 2022

Anti-Inflammatory Mechanisms of Total Flavonoids from against Influenza Virus-Induced Pneumonia by Integrating Network Pharmacology and Experimental Verification.

Evid Based Complement Alternat Med 2022 8;2022:2154485. Epub 2022 Jun 8.

Zhejiang Chinese Medical University, Hangzhou 310053, China.

Influenza virus is one of the most common infectious pathogens that could cause high morbidity and mortality in humans. However, the occurrence of drug resistance and serious complications extremely complicated the clinic therapy. is a natural medicinal plant used for treating various lung and gastrointestinal diseases, including viral infection, cough, chronic obstructive pulmonary disease, acute gastroenteritis, and diarrhoea. But the therapeutic effects of this herbal medicine had not been expounded clearly. In this study, a network pharmacology approach was employed to investigate the protective mechanism of total flavonoids from (MSTF) against influenza virus- (IAV-) induced acute lung damage and inflammation. The active compounds of MSTF were analyzed by LC-MS/MS and then evaluated according to their oral bioavailability and drug-likeness index. The potential targets of each active compound in MSTF were identified by using PharmMapper Server, whereas the potential genes involved in IAV infection were obtained from GeneGards. The results showed that luteoloside, apigenin, kaempherol, luteolin, mosloflavone I, and mosloflavone II were the main bioactive compounds found in MSTF. Primarily, 23 genes were identified as the targets of those five active compounds, which contributed to the inactivation of chemical carcinogenesis ROS, lipid and atherosclerosis, MAPK signaling pathway, pathways in cancer, PI3K-AKT signaling pathway, proteoglycans in cancer, and viral carcinogenesis. Finally, the animal experiments validated that MSTF improved IAV-induced acute lung inflammation via inhibiting MAPK, PI3K-AKT, and oxidant stress pathways. Therefore, our study demonstrated the potential inhibition of MSTF on viral pneumonia in mice and provided a strategy to characterize the molecular mechanism of traditional Chinese medicine by a combinative method using network pharmacology and experimental validation.
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http://dx.doi.org/10.1155/2022/2154485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200497PMC
June 2022

Analysis of factors influencing cervical lymph node metastasis of papillary thyroid carcinoma at each lateral level.

BMC Surg 2022 Jun 15;22(1):228. Epub 2022 Jun 15.

Xuan Wu Hospital of The Capital Medical University, Beijing, China.

Objective: To analyze the clinicopathological characteristics of patients with papillary thyroid carcinoma (PTC) and its influence on the distribution of lymph node metastasis at each lateral level of the neck to guide precise treatment of the lateral area.

Methods: The clinicopathological data of patients with PTC initially diagnosed and treated at our hospital from February 2014 to September 2021 were collected; the metastatic status of each lateral level was recorded, and correlations were analyzed.

Results: A total of 203 patients were enrolled in this study. There were 67 males and 136 females, with an average age of 41.1 years. In the lateral cervical area, lymph node metastasis was found at level IIa in 81 patients (39.9%); level III, 171 patients (84.2%); level IV, 122 patients (60%); and level Vb, 18 patients (8.9%). Correlation analysis showed that age (r = 0.198, P < 0.01) and sex (r = 0.196, P < 0.01) were weakly correlated with the number of positive lymph nodes in the central region. The tumor size (r = 0.164, P < 0.05) was weakly correlated with lymph node metastasis at level IV. The presence of multiple tumor foci was weakly correlated with lymph node metastasis at level IIa (r = 0.163, P < 0.05) and Vb (r = 0.143, P < 0.05). The tumor location (r = - 0.168, P < 0.05) was weakly correlated with lymph node metastasis at level III. The number of positive lymph nodes in the central region (r = 0.189, P < 0.01) was weakly correlated with lymph node metastasis at level IV. Binary logistic regression analysis showed that the risk of metastasis of multifocal tumors was higher than that of unifocal tumors by 1.958 times at level IIa (P = 0.021, OR = 1.958) and 2.929 times at level Vb (P = 0.049, OR = 2.929). The higher the tumor was located, the higher the risk of metastasis at level III (P = 0.014, OR = 0.563). Every additional positive lymph node in the central region increased the risk of metastasis at level IV by 1.126 times (P = 0.009, OR = 1.126).

Conclusions: For patients with pathological evidence of lateral metastasis, standard dissection of level IIa through Vb is recommended; selective dissection requires careful consideration. Patients with multifocal tumors have a high risk of metastasis at levels IIa and Vb, which requires special attention during the operation.
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http://dx.doi.org/10.1186/s12893-022-01678-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199251PMC
June 2022

Anthraquinone-Based Metal-Organic Frameworks as a Bifunctional Photocatalyst for C-H Activation.

Inorg Chem 2022 Jun 13;61(25):9493-9503. Epub 2022 Jun 13.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, P. R. China.

Metal-organic frameworks (MOFs) have gained attention as multifunctional catalytic platforms, allowing us to gain important insights into synergistically activating both C-H bonds and oxygen for improving oxidation. Herein, by ingenious incorporation of anthraquinone, we report an anthraquinone-based MOF as a bifunctional heterogeneous photocatalytic platform to simultaneously activate inert C(sp)-H bonds and oxygen for C-H bond oxidation. Making use of the rigid framework with the fixation and isolation effect, both a great chemical stability and bifunctional synergistic photocatalytic effects were obtained through the immobilization of anthraquinone into a MOF. Importantly, while decorating two carboxyl groups on anthraquinone, the carbonyl groups of anthraquinone photosensitizers were not involved in coordinating the self-assembly and orderly arranged on the wall of channels that were constructed through a π-π interaction between the anthraquinone moieties in the adjacent layers, which was beneficial to form and stabilize the excited-state radical intermediates in the molecule-fenced channels, and the close proximity between the catalytic sites and the substrates to abstract a hydrogen atom from the substrate through the hydrogen atom transfer process aimed at activating the inertness of C-H bonds. Moreover, high-density-distributed anthraquinone dyes in the confined channels would activate oxygen to form singlet oxygen (O) through an energy transfer pathway, further promoting inert C(sp)-H bond oxidation efficiency. Under visible light irradiation, this anthraquinone-based MOF was successfully applied to explore activation and oxidation of a series of substrates containing benzylic C(sp)-H bonds in the presence of air or oxygen to produce the corresponding carbonyl products. This bifunctional photocatalytic platform based on a heterogeneous MOF provides an available catalytic avenue to develop a scalable and sustainable synthetic strategy using green and sustainable oxygen as the potent oxidant.
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http://dx.doi.org/10.1021/acs.inorgchem.2c00441DOI Listing
June 2022

Distribution of Vaginal and Gut Microbiome in Advanced Maternal Age.

Front Cell Infect Microbiol 2022 27;12:819802. Epub 2022 May 27.

Department of Gynaecology and Obstetrics, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

The distribution of the microbiome in women with advanced maternal age (AMA) is poorly understood. To gain insight into this, the vaginal and gut microbiota of 62 women were sampled and sequenced using the 16S rRNA technique. These women were divided into three groups, namely, the AMA (age ≥ 35 years, = 13) group, the non-advanced maternal age (NMA) (age < 35 years, = 38) group, and the control group (non-pregnant healthy women, age >35 years, = 11). We found that the alpha diversity of vaginal microbiota in the AMA group significantly increased. However, the beta diversity significantly decreased in the AMA group compared with the control group. There was no significant difference in the diversity of gut microbiota among the three groups. The distributions of microbiota were significantly different among AMA, NMA, and control groups. In vaginal microbiota, the abundance of was higher in the pregnant groups. was significantly enriched in the AMA group. In gut microbiota, was significantly enriched in the AMA group. Vaginal and gut microbiota in women with AMA were noticeably different from the NMA and non-pregnant women, and this phenomenon is probably related to the increased risk of complications in women with AMA.
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http://dx.doi.org/10.3389/fcimb.2022.819802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186158PMC
May 2022

Efficacy and safety of apatinib as second or later-line therapy in extensive-stage small cell lung cancer: a prospective, exploratory, single-arm, multi-center clinical trial.

Transl Lung Cancer Res 2022 May;11(5):832-844

Department of Oncology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.

Background: A paucity of strategies exist for extensive-stage small cell lung cancer (ES-SCLC) patients who fail the first-line chemotherapy. Apatinib is a tyrosine kinase inhibitor (TKI) that selectively inhibits vascular endothelial growth factor receptor-2 (VEGFR-2), which has been demonstrated to have active anti-tumor activity in ES-SCLC when used only or combined with PD-1 inhibitors or chemotherapy with good tolerance. However, the efficacy and safety of apatinib monotherapy is unclear in second-line or beyond treatment of ES-SCLC.

Methods: In this prospective, exploratory, single-arm, multi-center study, eligible patients were aged 18 years or older with histologically confirmed ES-SCLC, and had progressed on, or were intolerant to previous systemic treatment. Patients received apatinib 500 mg (orally qd, every 4 weeks a cycle). The efficacy was assessed after 1 cycle and then every 2 cycles based on computed tomography imaging per the Response Evaluation Criteria in Solid Tumors (RECIST, version 1.1). The primary endpoint was progression-free survival (PFS). The adverse events (AEs) were assessed per the National Cancer Institute Common Terminology Criteria for Adverse Events 4.0 (NCI-CTCAE 4.0). This study is registered in the Chinese Clinical Trial Registry, number ChiCTR-OPC-17013964.

Results: From 28 July 2017 to 21 June 2019, 62 patients were screened for eligibility, among whom 57 patients were available for efficacy and safety analysis. The objective response rate (ORR) was 14.3% and disease control rate (DCR) was 79.6%. The median PFS was 5.6 months [95% confidence interval (CI): 3.3-8.0 months] and the median overall survival (OS) was 11.2 months (95% CI: 7.5-24.0 months). Among the participants who received apatinib as second-line treatment, the median PFS and OS were 6.1 months (95% CI: 2.6-7.6 months) and 12.0 months (95% CI: 7.9 months to not reached), respectively. The most common AEs of all grades were anemia (36.8%), hypertension (33.3%), fatigue (31.6%), blood bilirubin increased (22.8%), elevated transaminase (19.3%), and hand-foot syndrome (17.54%). Grade 3 AEs included 2 (3.5%) cases of hypertension and 1 (1.8%) case of fatigue. No grade 4/5 AEs were observed.

Conclusions: Apatinib showed encouraging anti-tumor activity in pretreated ES-SCLC patients with tolerable toxicities. Further larger scale studies are warranted to demonstrate the efficacy of apatinib.
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http://dx.doi.org/10.21037/tlcr-22-313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186180PMC
May 2022

Intestinal Continuity Alleviates Pediatric Intestinal Failure-Associated Liver Disease.

Front Surg 2022 16;9:881782. Epub 2022 May 16.

Division of Pediatric Gastroenterology and Nutrition; Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Type I short bowel syndrome (SBS) occurs after a critical reduction in the functional gut mass and resection of intestinal continuity after ileostomy or jejunostomy for necrotizing enterocolitis (NEC), intestinal atresia or other causes. SBS is often accompanied with intestinal failure-associated liver disease (IFALD) who requires long-term parenteral nutrition (PN). Our study aimed to observe the effect of intestinal continuity on the hepatic function of pediatric intestinal failure (IF) patients with type I SBS.

Methods: The pre-and post-anastomosis medical records of 35 pediatric patients with type I SBS from April 2013 to April 2019 were reviewed retrospectively. The average growth (cm/month) in the proximal and distal small bowel lengths was calculated as the growth in intestinal length (cm)/the duration (month) from enterostomy to anastomosis. The changes in hepatic function from enterostomy to anastomosis were evaluated by assessment of hepatic function before anastomosis for 6 weeks and after anastomosis for 4 weeks.

Results: The average growth in proximal intestinal length was 9.3 cm/month (±7.2) in neonates and 2.8 cm/month (1.3, 11.9) in infants and children, and in distal intestinal length was 1.5 cm/month (0, 2.7) in neonates and 0.4 cm/month (0, 1.4) in infants and children. The incidence of IFALD was 28.6% 1 month before anastomosis and 20.0% 1 month after anastomosis ( < 0.05).

Conclusion: In pediatric type I SBS with IFALD, restoration of intestinal continuity may alleviate liver injury. There was an intestinal compensatory effect on the growth in the intestinal length after resection, and better results were seen in neonates in terms of intestinal length growth.
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http://dx.doi.org/10.3389/fsurg.2022.881782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149254PMC
May 2022

Changes of Tumor Markers in Patients with Breast Cancer during Postoperative Adjuvant Chemotherapy.

Dis Markers 2022 19;2022:7739777. Epub 2022 May 19.

Department of General Surgery, Xuanwu Hospital, Capital Medical University, China.

Objective: Serum tumor marker (STM) elevation can detect metastasis earlier than imaging diagnosis and, although not recommended by guidelines, is still widely used in clinical practice for postoperative follow-up of breast cancer patients. The purpose of this study was to investigate the change rules of CEA and CA153 in patients with HER2-negative breast cancer during postoperative adjuvant chemotherapy and their influencing factors.

Materials And Methods: The medical records of patients with HER2-negative early breast cancer who visited Xuanwu Hospital from September 2018 to June 2021 were retrospectively analyzed. Demographic characteristics and baseline data of CEA and CA153 at initial diagnosis were collected. Data of CEA, CA153, biochemistry (including ALT, AST, rGT, triglycerides, cholesterol, and blood glucose) and blood routine (including white blood cells, neutrophils, monocytes, lymphocytes, and platelets) were also collected before chemotherapy, at the end of chemotherapy and more than 3 months after the end of chemotherapy. LY/MONO, NEUT/LY, PLT/LY, and systemic immune inflammation index (SII) were calculated and statistically analyzed using SPSSAU software.

Results: A total of 90 patients were enrolled, all of whom were female, with a mean age of 55.11 ± 10.60 y. The value of CEA at initial diagnosis was 2.10 ± 1.11 ng/mL, and high expression was mostly correlated with past history of chronic diseases and tumor lymph node metastasis; the value of CA153 was 11.80 ± 6.60 U/mL, and high expression was correlated with high SII at initial diagnosis. Surgery did not affect the values of serum CEA and CA153. At the end of chemotherapy, CEA and CA153 were 2.68 ± 1.34 ng/mL and 18.51 ± 8.50 U/mL, respectively, which were significantly increased compared with those before chemotherapy, and were linearly correlated with the values before chemotherapy. They decreased (CEA 2.45 ± 1.19 ng/mL, CA153 10.87 ± 5.96 U/mL) again three months after the end of chemotherapy, manifested as "spiking" phenomenon, which was associated with lymph node metastasis at diagnosis, body metabolic disorders, and chronic inflammatory status.

Conclusion: CEA and CA153 were increased presenting as "spiking" phenomena in patients with early HER2-negative breast cancer during postoperative adjuvant chemotherapy, and the peak of increase was linearly correlated with the indicators before chemotherapy. Clinical attention should be paid to this change to avoid excessive diagnosis and treatment leading to medical resource consumption.
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http://dx.doi.org/10.1155/2022/7739777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135560PMC
June 2022

Mptx2 defends against peritoneal infection by methicillin-resistant staphylococcus aureus.

Int Immunopharmacol 2022 Jul 21;108:108856. Epub 2022 May 21.

Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Department of Pediatric Surgery, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Institute of Pediatric Research, Shanghai, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China. Electronic address:

Methicillin-resistant Staphylococcus aureus (MRSA) has an increasing prevalence of multi-drug resistance. There is an urgent need for developing novel approaches to combat MRSA infection. Mucosal pentraxin 2 (Mptx2) is predicted to be a member of the pentraxin family, but its biological function is still unknown. This study is aimed to explore the roles of Mptx2 in MRSA-associated peritoneal infection. The recombinant Mptx2 protein is used to evaluate its antibacterial activity. Biofilm formation assay and macrophage phagocytic experiment are performed to explore the involved mechanisms. The effects of Mptx2 on peritoneal infection are investigated in a MRSA-induced peritoneal infected model. We here show that addition of Mptx2 suppresses the growth and biofilm formation of MRSA in vitro. Enzyme-linked immunosorbent assay (ELISA) binding analysis shows that Mptx2 protein directly binds to the MRSA. Additionally, Mptx2 supplementation promotes macrophages to phagocytize and clear the MRSA. In the MRSA-infected peritonitis model, Mptx2 administration reduces MRSA loading in peritoneal organs and alleviates peritoneal damage. Mptx2 knockout aggravates MRSA infection-induced peritoneal injury. In conclusion, our findings reveal that Mptx2 has bactericidal activity against MRSA both in vitro and in vivo, which may shed light on the discovery and development of novel strategies for MRSA-infected peritonitis.
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http://dx.doi.org/10.1016/j.intimp.2022.108856DOI Listing
July 2022

Electronic Orbital Alignment and Hierarchical Phonon Scattering Enabling High Thermoelectric Performance p-Type MgSb Zintl Compounds.

Research (Wash D C) 2022 29;2022:9842949. Epub 2022 Apr 29.

State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin 150001, China.

Environmentally friendly MgSb-based materials have drawn intensive attention owing to their promising thermoelectric performance. In this work, the electrical properties of p-type MgSb are dramatically optimized by the regulation of Mg deficiency. Then, we, for the first time, found that Zn substitution at the Mg2 site leads to the alignment of and orbital, resulting in a high band degeneracy and the dramatically enhanced Seebeck coefficient, demonstrated by the DFT calculations and electronic properties measurement. Moreover, Zn alloying decreases Mg1 (Zn) vacancies formation energy and in turn increases Mg (Zn) vacancies and optimizes the carrier concentration. Simultaneously, the Mg/Zn substitutions, Mg vacancies, and porosity structure suppress the phonon transport in a broader frequency range, leading to a low lattice thermal conductivity of ~0.47 W m K at 773 K. Finally, a high of ~0.87 at 773 K was obtained for MgNaZnSb, exceeding most of the previously reported p-type MgSb compounds. Our results further demonstrate the promising prospects of p-type MgSb-based material in the field of mid-temperature heat recovery.
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http://dx.doi.org/10.34133/2022/9842949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079362PMC
April 2022
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