Publications by authors named "Wayne M Barnes"

12 Publications

  • Page 1 of 1

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement.

Front Bioeng Biotechnol 2020 14;8:553474. Epub 2021 Jan 14.

DNA Polymerase Technology, Inc., St. Louis, MO, United States.

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January 2021

A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.

Biochemistry 2015 Jan 9;54(3):881-9. Epub 2015 Jan 9.

Department of Biology and ‡Department of Chemistry, University of Richmond , Richmond, Virginia 23173, United States.

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January 2015

Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq.

J Mol Diagn 2010 Mar 14;12(2):152-61. Epub 2010 Jan 14.

DNA Polymerase Technology Inc., 1508 South Grand Blvd., St. Louis, MO 63104, USA.

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March 2010

Streamlined gene assembly PCR.

CSH Protoc 2008 Mar 1;2008:pdb.prot4862. Epub 2008 Mar 1.

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March 2008

Long and accurate PCR.

Authors:
Wayne M Barnes

CSH Protoc 2006 Jun 1;2006(1). Epub 2006 Jun 1.

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June 2006

The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition.

J Bacteriol 2004 Jun;186(11):3547-60

Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, Medical School, University of Birmingham, Birmingham B15 2TT, United Kingdom.

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June 2004

Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.

Nucleic Acids Res 2003 Nov;31(21):6139-47

DNA Polymerase Technology Inc., 1508 South Grand Avenue, St Louis, MO 63104, USA.

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November 2003

Universal PCR genotyping assay that achieves single copy sensitivity with any primer pair.

Transgenic Res 2003 Aug;12(4):521-2

Department of Pediatrics, Washington University School of Medicine, Campus Box 8208, 660 South Euclid Avenue, Saint Louis, MO 63110, USA.

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August 2003

Mammalian microevolution: Rapid change in mouse mitochondrial DNA.

Nature 2003 May;423(6938):397

Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA.

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May 2003

Magnesium precipitate hot start method for PCR.

Mol Cell Probes 2002 Jun;16(3):167-71

DNA Polymerase Technology, Inc., St. Louis, MO 63104, USA.

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June 2002