Publications by authors named "Wanying He"

8 Publications

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Limited hydrolysis and conjugation of zein with chitosan oligosaccharide by enzymatic reaction to improve functional properties.

Food Chem 2021 Jun 12;348:129035. Epub 2021 Jan 12.

Purdue University, Department of Food Science, West Lafayette, IN 47907, United States; Purdue University, Whistler Center for Carbohydrate Research, West Lafayette, IN 47907, United States. Electronic address:

In order to improve its aqueous solubility and emulsifying function, zein was partially hydrolyzed by trypsin and conjugated to chitosan oligosaccharide lactate by transglutaminase. Hydrolysis and covalent linkage to chitosan oligosaccharide was confirmed by free amine content, gel electrophoresis, and infrared spectroscopy. Enzymatic glycosylation was optimized at pH 6, 44 °C, and 4 h to bind approximately 95% of the free amines in the hydrolysates to chitosan oligosaccharide. Hydrolysis and conjugation increased solubility of zein by 47.60% and 72.93%. Hydrolysis and conjugation also decreased surface hydrophobicity by more than 20% and more than doubled emulsifying activity index, emulsion stability index, and foaming capacity. This enzymatic modification has potential to be applied to improve functional properties of other prolamins.
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http://dx.doi.org/10.1016/j.foodchem.2021.129035DOI Listing
June 2021

Characteristics of the In Situ Stress Field in the Yadian Coal Mine, Central China: Implications for Roadway Differential Deformation.

ACS Omega 2021 Jan 24;6(1):739-746. Epub 2020 Dec 24.

Bin County Coal Co., Ltd., Xianyang 712000, China.

To study the in situ stress mechanism for different deformations of roadways in different directions, in this paper, we used the method of stress relief with a hollow core to measure the in situ stress of roadways with different degrees of deformation in the Yadian coal mine. The results showed that the in situ stress field was generally σ > σ > σ, indicating a typical reverse faulting stress regime. The present in situ stress field was the main factor for roadway differential deformation. The σ orientations were ESE and ENE-trending, which were consistent with the regional tectonic stress field in North China. The instrument for monitoring the abscission layer deformation indicated that the NS-trending roadway (cut-hole) deformation time approached stability after approximately 1-2 months, while it was 5-10 days for its EW-trending counterpart. The study of stress gradient shows that the stress state of the Ordos Basin has changed at a depth of 600 m, which is related to multiple tectonic events from the Late Paleozoic to Cenozoic in the Ordos Basin. The comprehensive analysis results show that the differential deformation of roadways is affected by many factors, such as the buried depth of the stratum, lithology and structural characteristics, fracture development degree, mining influence, composite deformation mechanics mechanism of weak swelling rock stratum, in situ stress field, tectonic stress mechanism, fracture development, etc.
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http://dx.doi.org/10.1021/acsomega.0c05205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807791PMC
January 2021

Regulatory Roles of Pectin Oligosaccharides on Immunoglobulin Production in Healthy Mice Mediated by Gut Microbiota.

Mol Nutr Food Res 2019 07 19;63(14):e1801363. Epub 2019 Jun 19.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.

Scope: The prebiotic regulation of the gut microbiota is a promising strategy to induce protective humoral and mucosal immune responses. The potential immune-improving effects of pectin oligosaccharides (POS) in healthy mice and the potential mechanism mediated by specific intestinal bacteria are investigated.

Methods And Results: POS is prepared using a hydrogen-peroxide-assisted degradation. Mice that consumed diets containing POS are tested for microbial community shifts, short-chain fatty acids (SCFAs), and immunoglobulin (Ig) production using quantitative real-time polymerase chain reaction, gas chromatography, and ELISA kits. Pearson's correlation analyses are performed between Ig production and specific intestinal bacteria or SCFAs. POS treatment significantly improves the growth of healthy mice. Moreover, 4-week POS administration results in a profound change in intestinal microbial composition and a significantly higher fecal concentration of acetate, which leads to substantial increases of the levels of fecal secretory immunoglobulin A and serum IgG.

Conclusions: The results suggest that the inclusion of POS in a diet can increase Ig production and optimize the composition of the gut microbiota. A significant correlation is observed between changes in Ig production and specific intestinal bacteria or acetate, providing insight into the mechanism of POS as a potential immune-enhancing supplement.
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http://dx.doi.org/10.1002/mnfr.201801363DOI Listing
July 2019

Enhanced local cancer therapy using a CA4P and CDDP co-loaded polypeptide gel depot.

Biomater Sci 2019 Feb;7(3):860-866

Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

Cancer combination therapy based on drug co-delivery systems provides an effective strategy for enhancing treatment efficacy and reducing side effects. In this work, a new strategy through co-delivery of combretastatin A4 disodium phosphate (CA4P) and cisplatin (CDDP) was developed for the local treatment of colon cancer, through an in situ thermo-gelling hydrogel (mPEG-b-PELG). The results indicated that this material possessed concentration-dependent thermogelling properties and tunable in vivo biodegradability. Also, the drug loaded gel could regulate the in vitro drug release behaviors of both CDDP and CA4P, which promoted the in vivo vessel disrupting effects of CA4P compared with a free drug after local treatment for 48 h. Although the drug co-loaded gel induced less in vitro cell death compared with the free drug co-treated group, this drug co-loaded gel depot showed the highest antitumor efficacy compared with the other experimental groups after peritumoral injection toward C26 tumor bearing mice.
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http://dx.doi.org/10.1039/c8bm01442fDOI Listing
February 2019

Anti-Oxidant and Anti-Melanogenic Properties of Essential Oil from Peel of Pomelo cv. Guan Xi.

Molecules 2019 Jan 10;24(2). Epub 2019 Jan 10.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Here, we investigated the anti-oxidant and anti-melanogenic effects of pomelo peel essential oil (PPEO) from pomelo cv. Guan Xi. The volatile chemical composition of PPEO was analyzed with gas chromatography⁻mass spectrometry (GC/MS). The most abundant component of PPEO was limonene (55.92%), followed by β-myrcene (31.17%), and β-pinene (3.16%). PPEO showed strong anti-oxidant activities against 1,1-diphenyl-2-picryhydrazyl (DPPH) (,2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) (, and superoxide anion free radicals. Based on the B16 melanoma cell system, the effects of PPEO on the viability and morphology of B16 cells and the production of melanin were evaluated. The results revealed that PPEO at concentrations below 50 μg/mL could decrease the melanin content without affecting cell viability and morphology. Intracellular tyrosinase (TYR) activity and Western blot analysis showed that PPEO could down-regulate the expression level of TYR in B16 cells and dose-dependently inhibit TYR activity (by a maximum of 64.54%). Meanwhile, the main active components responsible for the effect are citral and β-myrcene. In conclusion, PPEO has good anti-oxidant and anti-melanogenic activity, and thus can be widely used as a natural antioxidant in the food, pharmaceutical, and cosmetic industries.
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http://dx.doi.org/10.3390/molecules24020242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359654PMC
January 2019

Kinesio taping is superior to other taping methods in ankle functional performance improvement: a systematic review and meta-analysis.

Clin Rehabil 2018 Nov 18;32(11):1472-1481. Epub 2018 Jul 18.

3 Zhujiang Hospital of Southern Medical University, Guangzhou, China.

Objective:: To compare the effect of Kinesio taping on ankle functional performance with that of other taping methods (non-elastic taping) in healthy individuals and patients with ankle sprain.

Methods:: A search was performed in electronic databases (MEDLINE, Embase, Cochrane Library, and China National Knowledge Infrastructure) for studies published up to 31 March 2018 using the following keywords: ankle, Kinesio taping, KT, and tape. Studies on ankle functional performance were selected, and data on Star Excursion Balance Test results, vertical jump height, and range of motion were extracted. Meta-analyses (where appropriate and possible) using either fixed or random effects model, standardized mean differences, and tests of heterogeneity were performed.

Results:: Ten studies fulfilled the inclusion criteria. The Star Excursion Balance Test results indicated that Kinesio taping was superior to other taping methods (placebo taping or tension-free taping). The mean difference was 3.2 (95% confidence interval (CI): 0.84-5.59, I = 5%, P = 0.008). In studies in which vertical jump height was measured, a negative effect was observed for other taping methods (athletic taping or placebo taping), but not for Kinesio taping (mean difference = 1.06, 95% CI: 0.19-0.93, I = 15%, P = 0.02). Both Kinesio taping and common taping had no significant effect on range of motion, with no differences between these methods ( I = 0%, P = 0.80).

Conclusion:: Kinesio taping is superior to other taping methods (athletic taping) in ankle functional performance improvement.
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http://dx.doi.org/10.1177/0269215518780443DOI Listing
November 2018

Production of nano bacterial cellulose from beverage industrial waste of citrus peel and pomace using Komagataeibacter xylinus.

Carbohydr Polym 2016 Oct 16;151:1068-1072. Epub 2016 Jun 16.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China. Electronic address:

Bacterial cellulose (BC) is a high-purity and robust cellulose that is utilised in medicine, consumer goods, and industrial practices. The present study aimed to investigate the suitability of beverage industrial waste for the production of BC by Komagataeibacter xylinus CICC No. 10529 and to study the structural properties of BC films in both citrus peel and pomace enzymolysis (CPPE) and Hestrin-Schramm (HS, Hestrin & Schramm, 1954) media. Under similar experimental conditions, the yield of BC from CPPE medium was 5.7±0.7g/L, which was higher than from HS medium (3.9±0.6g/L). To evaluate the structure of BC, fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and colour evaluation using a chroma meter were utilised. The average diameters of BC, obtained from CPPE and HS mediums, were 50nm and 60nm, respectively. The crystallinity index of BC from the CPPE medium was approximately 63%, which was lower than BC produced from the HS medium (65%). The two varieties of BC showed no significant differences in relation to their colour parameters. Therefore, BC production from CPPE medium had similar properties to BC from HS medium, but it is more environmentally friendly and cheaper to produce.
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http://dx.doi.org/10.1016/j.carbpol.2016.06.062DOI Listing
October 2016

pH-Triggered Charge-Reversal Polyurethane Micelles for Controlled Release of Doxorubicin.

Macromol Biosci 2016 06 3;16(6):925-35. Epub 2016 Feb 3.

Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

A series of pH-triggered charge-reversal polyurethane copolymers (PS-PUs) containing methoxyl-poly(ethylene glycol) (mPEG), carboxylic acid groups, and piperazine groups is presented in this work. The obtained PS-PUs copolymers can form into stable micelles at pH 7.4, which response to a narrow pH change (5.5-7.5) and show a tunable pH-triggered charge-reversal property. Doxorubicin (DOX) is encapsulated into the PS-PU micelles as a model drug. The drug release of DOX-loaded PS-PU micelles shows an obviously stepped-up with reducing the pH. Meanwhile, it is found that the charge-reversal property can improve the cellular uptake behavior and intracellular drug release in both HeLa cells and MCF-7 cells. Additionally, the time-dependent cytotoxicity of the DOX-loaded PS-PU micelles is confirmed by MTT assay. Attributed to the tunable charge-reversal property through changing the molar ratio of piperazine/carboxyl, the PS-PU micelles will be a potential candidate as an intelligent drug delivery system in future studies.
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http://dx.doi.org/10.1002/mabi.201500358DOI Listing
June 2016