Publications by authors named "Wanwei Hou"

3 Publications

  • Page 1 of 1

Development and application of the Faba_bean_130K targeted next-generation sequencing SNP genotyping platform based on transcriptome sequencing.

Theor Appl Genet 2021 Jun 12. Epub 2021 Jun 12.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Key Message: Large-scale faba bean transcriptome data are available, and the first genotyping platform based on liquid-phase probe targeted capture technology was developed for genetic and molecular breeding studies. Faba bean (Vicia faba L., 2n = 12) is an important food legume crop that is widely grown for multiple uses worldwide. However, no reference genome is currently available due to its very large genome size (approximately 13 Gb) and limited single nucleotide polymorphism (SNP) markers as well as highly efficient genotyping tools have been reported for faba bean. In this study, 16.7 billion clean reads were obtained from transcriptome libraries of flowers and leaves of 102 global faba bean accessions. A total of 243,120 unigenes were de novo assembled and functionally annotated. Moreover, a total of 1,579,411 SNPs were identified and further filtered according to a selection pipeline to develop a high-throughput, flexible, low-cost Faba_bean_130K targeted next-generation sequencing (TNGS) genotyping platform. A set of 69 Chinese faba bean accessions were genotyped with the TNGS genotyping platform, and the average mapping rate of captured reads to reference transcripts was 93.14%, of which 53.23% were located in the targeted regions. The TNGS genotyping results were validated by Sanger sequencing and the average consistency rate reached 93.6%. Comprehensive population genetic analysis was performed on the 69 Chinese faba bean accessions and identified four genetic subgroups correlated with the geographic distribution. This study provides valuable genomic resources and a reliable genotyping tool that could be implemented in genetic and molecular breeding studies to accelerate new cultivar development and improvement in faba bean.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00122-021-03885-0DOI Listing
June 2021

Development of Nanocomplexes for Curcumin Vehiculization Using Ovalbumin and Sodium Alginate as Building Blocks: Improved Stability, Bioaccessibility, and Antioxidant Activity.

J Agric Food Chem 2019 Jan 31;67(1):379-390. Epub 2018 Dec 31.

Department of Functional Food and Bio-Active Compounds, Institute of Agro-Product Processing , Jiangsu Academy of Agricultural Sciences , 50 Zhongling Street , Nanjing 210014 , China.

Type I (Complex I) and type II nanocomplexes (Complex II) were created in this work for curcumin (Cur) delivery using ovalbumin (OVA, 1.0% w/w) and sodium alginate (ALG, 0.5% w/w) as building blocks. OVA was heated at 90 °C for 5 min at pH 7.0 and then coated with ALG at pH 4.2 to produce Complex I; OVA-ALG electrostatic complex was created at pH 4.0, which was treated at 90 °C for 5 min thereafter yielding Complex II. Complex I presented an irregular elliptical shape with a diameter of ∼250 nm, whereas Complex II adopted a defined spherical structure of a smaller size (∼200 nm). Complex II did not dissociate at the pH range of 5-7, which was different from Complex I. Cur was loaded into the nonpolar matrix of nanocomplexes through hydrogen bonding and hydrophobic interactions, and Complex II displayed a higher loading capacity than Complex I. Nanocomplexes were resistant to pepsinolysis during simulated gastrointestinal digestion, which enhanced the stability and controlled release of loaded Cur, thereby improving Cur bioaccessibility from ∼20% (free form) to ∼60%. Additionally, nanocomplexes contributed to the cellular antioxidant activity (CAA) of Cur by promoting its cellular uptake. The CAA of Cur was also better preserved in nanocomplexes especially in Complex II after digestion owing to the increased stability and bioaccessibility. Results from this work highlighted the effect of nanocomplex encapsulation on the performance of Cur and revealed the critical role of preparation method in the physicochemical attributes of nanocomplexes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.8b02567DOI Listing
January 2019

Structure and function of seed storage proteins in faba bean (Vicia faba L.).

3 Biotech 2017 May 27;7(1):74. Epub 2017 Apr 27.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Qinghai Academy of Agricultural and Forestry Science, Xining, Qinghai, 810016, People's Republic of China.

The protein subunit is the most important basic unit of protein, and its study can unravel the structure and function of seed storage proteins in faba bean. In this study, we identified six specific protein subunits in Faba bean (cv. Qinghai 13) combining liquid chromatography (LC), liquid chromatography-electronic spray ionization mass (LC-ESI-MS/MS) and bio-information technology. The results suggested a diversity of seed storage proteins in faba bean, and a total of 16 proteins (four GroEL molecular chaperones and 12 plant-specific proteins) were identified from 97-, 96-, 64-, 47-, 42-, and 38-kD-specific protein subunits in faba bean based on the peptide sequence. We also analyzed the composition and abundance of the amino acids, the physicochemical characteristics, secondary structure, three-dimensional structure, transmembrane domain, and possible subcellular localization of these identified proteins in faba bean seed, and finally predicted function and structure. The three-dimensional structures were generated based on homologous modeling, and the protein function was analyzed based on the annotation from the non-redundant protein database (NR database, NCBI) and function analysis of optimal modeling. The objective of this study was to identify the seed storage proteins in faba bean and confirm the structure and function of these proteins. Our results can be useful for the study of protein nutrition and achieve breeding goals for optimal protein quality in faba bean.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13205-017-0691-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428115PMC
May 2017
-->