Publications by authors named "Wanjun Hu"

15 Publications

  • Page 1 of 1

Expansion of Ovarian Cancer Stem-like Cells in Poly(ethylene glycol)-Cross-Linked Poly(methyl vinyl ether--maleic acid) and Alginate Double-Network Hydrogels.

ACS Biomater Sci Eng 2020 06 21;6(6):3310-3326. Epub 2020 Apr 21.

State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

A better understanding of cancer stem cells (CSCs) is essential for research on cancer therapy and drug resistance. Currently, increasingly more investigations are focused on obtaining CSCs to study the mechanism of their enhanced malignancy. In this work, three kinds of double-network hydrogels (PEMM/alginate), consisting of poly(ethylene glycol) (PEG) covalently cross-linked poly(methyl vinyl ether--maleic acid) (P(MVE--MA)) (network 1, denoted as PEMM) and Sr (or Ca, Fe) ionically cross-linked alginates (network 2, denoted as SrAlg, CaAlg, or FeAlg), were prepared. The stiffness, morphology, and components of the PEMM/alginate hydrogels were systematically investigated to understand their effects on CSC enrichment. Only the PEMM/FeAlg hydrogels could support the long-term growth, proliferation, and spheroid formation of SK-OV-3 cells. The expression of CSC-related markers was evaluated with the levels of protein and gene at different stages. The cell spheroids cultured in the PEMM/FeAlg hydrogels acquired certain CSC-like properties, thus drug resistance was enhanced, especially in the PEMM-1/FeAlg hydrogel. tumorigenicity experiments also confirmed the presence of more CSCs in the PEMM-1/FeAlg hydrogel. The results suggest that matrix stiffness, morphology, and cations act synergistically on the regulation of the epithelial-mesenchymal transition (EMT), interleukin-6 (IL-6), and Wnt pathways, affecting the invasiveness of ovarian cancer and the conversion of the non-CSCs into CSCs. The PEMM-1/FeAlg hydrogel with lower elastic modulus, a more macroporous morphology, and higher swelling rate can significantly enhance the stemness, malignancy, and tumorigenicity of SK-OV-3 cells.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01967DOI Listing
June 2020

Combination of Polypropylene Mesh and in Situ Injectable Mussel-Inspired Hydrogel in Laparoscopic Hernia Repair for Preventing Post-Surgical Adhesions in the Piglet Model.

ACS Biomater Sci Eng 2020 03 12;6(3):1735-1743. Epub 2020 Feb 12.

Department of General Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.

Polypropylene (PP) mesh has been used successfully for a long time in clinical practice as an impressive prosthesis for ventral hernia repair. To utilize a physical barrier for separating mesh from viscera is a general approach for preventing adhesions in clinical practice. However, a serious abdominal adhesion between the mesh and viscera can possibly occur post-hernia, especially with the small intestine; this can lead to a series of complications, such as chronic pain, intestinal obstruction, and fistula. Thus, determining how to prevent abdominal adhesions between the mesh and viscera is still an urgent clinical problem. In this study, a dopamine-functionalized polysaccharide derivative (oxidized-carboxymethylcellulose--dopamine, OCMC-DA) was synthesized; this was blended with carboxymethylchitosan (CMCS) to form a hydrogel (OCMC-DA/CMCS) in situ at the appropriate time. The physical and chemical properties of the hydrogel were characterized successfully, and its excellent biocompatibility was presented by the in vitro cell test. The combination of this hydrogel and PP mesh was used in laparoscopic surgery for repairing the abdominal wall defect, where the hydrogel could become fixed in situ on the PP mesh to form an anti-adhesion gel-mesh. The results showed that the gel-mesh could prevent abdominal adhesions effectively in the piglet model. Moreover, the histology and immunohistochemical staining proved that the gel-mesh could effectively alleviate the inflammation reaction and deposition of collagen around the mesh, and it did not disturb the integration between mesh and abdominal wall. Thus, the gel-mesh has superior tissue compatibility.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01333DOI Listing
March 2020

Effect of RGD content in poly(ethylene glycol)-crosslinked poly(methyl vinyl ether-alt-maleic acid) hydrogels on the expansion of ovarian cancer stem-like cells.

Mater Sci Eng C Mater Biol Appl 2021 Jan 3;118:111477. Epub 2020 Sep 3.

State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address:

The extracellular matrix (ECM) affects cell behaviors, such as survival, proliferation, motility, invasion, and differentiation. The arginine-glycine-aspartic acid (RGD) sequence is present in several ECM proteins, such as fibronectin, collagen type I, fibrinogen, laminin, vitronectin, and osteopontin. It is very critical to develop ECM-like substrates with well-controlled features for the investigation of influence of RGD on the behavior of tumor cells. In this study, poly(ethylene glycol) (PEG)-crosslinked poly(methyl vinyl ether-alt-maleic acid) (P(MVE-alt-MA)) hydrogels (PEMM) with different RGD contents were synthesized, fully characterized, and established as in vitro culture platforms to investigate the effects of RGD content on cancer stem cell (CSC) enrichment. The morphology, proliferation, and viability of SK-OV-3 ovarian cancer cells cultured on hydrogels with different RGD contents, the expression of CSC markers and malignant signaling pathway-related genes, and drug resistance were systematically evaluated. The cell aggregates formed on the hydrogel surface with a lower RGD content acquired certain CSC-like properties, thus drug resistance was enhanced. In contrast, the drug sensitivity of cells on the higher RGD content surface increased because of less CSC-like properties. However, the presence of RGD in the stiff hydrogels (PEMM2) had less effect on the stemness expression than did its presence in the soft hydrogels (PEMM1). The results suggest that RGD content and matrix stiffness can lead to synergetic effects on the expression of cancer cell stemness and the epithelial-mesenchymal transition (EMT), interleukin-6 (IL-6), and Wnt pathways.
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http://dx.doi.org/10.1016/j.msec.2020.111477DOI Listing
January 2021

Mussel-inspired hybrid network hydrogel for continuous adhesion in water.

J Mater Chem B 2020 03;8(10):2148-2154

State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

The mussel-inspired catechol-based strategy has been widely used in the development of adhesives. However, the properties of the obtained adhesives were still severely limited in a humid environment, particularly in water. In this study, a facile and versatile approach was proposed to prepare an underwater adhesion hydrogel. First, dopamine (DA) was grafted on oxidized carboxymethylcellulose (OCMC) to obtain dopamine-grafted oxidized carboxymethylcellulose (OCMC-DA). Second, the acrylamide (AM) monomer was conjugated with OCMC-DA by a Schiff base reaction, and then polymerized to form an OCMC-DA/PAM hydrogel. The properties of the resulting hydrogel have been fully characterized. The underwater adhesion strength of the hydrogel can reach as high as 86.3 ± 7.2 kPa and reduced to 43 ± 3.4 kPa after being immersed in water for 9 days. More remarkably, we found that the maximal adhesion strength was shown when the G' and G'' of the hydrogel were very close. Moreover, we demonstrated the mechanical properties of our fabricated hydrogel by compressive tests and rheological analysis. The adhesive hydrogel also exhibits excellent biocompatibility.
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http://dx.doi.org/10.1039/c9tb02863cDOI Listing
March 2020

Tentative epidemiologic cut-off value and resistant characteristic detection of apramycin against Escherichia coli from chickens.

FEMS Microbiol Lett 2019 08;366(16)

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China.

Escherichia coli are important foodborne zoonotic pathogens. Apramycin is a key aminoglycoside antibiotic used by veterinarians against E. coli. This study was conducted to establish the epidemiological cut-off value (ECV) and resistant characteristics of apramycin against E. coli. In this study, 1412 clinical isolates of E. coli from chickens in China were characterized. Minimum inhibitory concentrations (MICs) of apramycin were assessed by broth microdilution method. MIC50 and MIC90 for apramycin against E. coli (0.5-256 µg/mL) were 8 and 16 µg/mL, respectively. In this study, the tentative ECV was determined to be 16 µg/mL by the statistical method and 32 µg/mL by ECOFFinder software. Besides, the percentages of aac(3)-IV positive strains ascended with the increase of MIC values of apramycin, and the gene npmA was detected in strains with higher MICs. Sixteen apramycin highly resistant strains displayed multiple drug resistance (100%) to amoxicillin, ampicillin, gentamicin, doxycycline, tetracycline, trimethoprim and florfenicol, while most of them were susceptible to amikacin and spectinomycin. In summary, the tentative ECV of apramycin against E. coli was recommended to be 16 µg/mL.
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http://dx.doi.org/10.1093/femsle/fnz196DOI Listing
August 2019

Population pharmacokinetics for danofloxacin in the intestinal contents of healthy and infected chickens.

J Vet Pharmacol Ther 2019 Sep 19;42(5):556-563. Epub 2019 Aug 19.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Avian pathogenic Escherichia coli could cause localized and systemic infection in the poultry, and danofloxacin is usually used to treat avian colibacillosis through oral administration. To promote prudent use of danofloxacin and reduce the emergence of drug-resistant E. coli strains, it is necessary to understand the population pharmacokinetics (PopPK) of danofloxacin in chicken intestines. In this study, reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection was used to detect the concentrations of danofloxacin in the contents of duodenum, jejunum, and ileum of the healthy and infected chickens after single oral administration (5 mg/kg body weight). Then, the PopPK of danofloxacin in intestines were analyzed using NONMEM software. As a result, a two-compartment PK model best described the time-concentration profile of duodenal, jejunal, and ileal contents. Interestingly, absorption rate (K ), distribution volume (V), and clearance (CL) for danofloxacin from duodenal, jejunal to ileal contents were sequentially decreased in the healthy chickens. However, the trend of K , V, and CL of danofloxacin was changed dramatically in the intestine of infected chickens. K and V of danofloxacin in the jejunum were higher than in the ileum and duodenum. Compared with healthy chickens, K and V of danofloxacin in the duodenum decreased significantly, while increased in jejunum, respectively. It has been noted that K decreased and V increased in the ileum of infected chickens. Besides, CL in the duodenum, jejunum, and ileum of infected chickens was, respectively, lower than those of healthy chickens. Interestingly, the relative bioavailability (F) of danofloxacin in the ileum was relatively higher in both healthy and infected chickens. In addition, F in the duodenal, jejunal, and ileal contents of infected chickens was respectively higher than healthy chickens. In summary, the PopPK for danofloxacin in infected chicken intestines was quite different from healthy chickens. The absorption, distribution, and clearance of danofloxacin in healthy chickens decreased from duodenum to jejunum and to ileum. Moreover, the pharmacokinetic characteristics in the intestine of infected chickens changed significantly, and the pharmacokinetic characteristics in the ileum can be used as a representative of all intestinal segments.
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http://dx.doi.org/10.1111/jvp.12799DOI Listing
September 2019

Mussel-inspired copolymer-coated polypropylene mesh with anti-adhesion efficiency for abdominal wall defect repair.

Biomater Sci 2019 Mar;7(4):1323-1334

State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Polypropylene (PP) meshes are one of the most commonly used prosthesis materials in repairing abdominal wall defects. However, their application is usually limited due to possible serious abdominal adhesions between the mesh and the viscera. Instilling PP meshes with excellent anti-adhesion characteristics is still a formidable challenge. In this work, in order to prevent intestinal adhesion to the PP mesh, an effective method was developed to prepare anti-adhesive PP meshes, which was inspired by mussel adhesive proteins. A functional monomer, namely, dopamine methacrylamide, was first synthesized. Then, it was copolymerized with poly(ethylene glycol) methacrylate on the surface of O2-plasma-treated PP (OPP) meshes to form comb-like copolymer poly[poly(ethylene glycol) methacrylate-co-dopamine methacrylamide] (PEDMA), which was simultaneously grafted in situ on the OPP mesh surface through the catechol group of PEDMA, subsequently yielding an anti-adhesive PP mesh (OPP-g-PEDMA). The properties of PEDMA and OPP-g-PEDMA meshes were characterized by NMR, GPC, TGA, FTIR, XPS, SEM, and water contact angle measurements. NIH-3T3 cells were employed to assess the cytocompatibility of OPP-g-PEDMA in vitro. Furthermore, the rat abdominal wall defect model was used to evaluate the efficacy of adhesion prevention. The results show that OPP-g-PEDMA not only possesses fantastic biocompatibility but also satisfactory anti-adhesion property involving minimal chronic inflammation, as well as lower adhesion formation rate and adhesion tenacity scores (less than 1.0). This type of OPP-g-PEDMA mesh is a promising candidate in effectively preventing peritoneal adhesion during abdominal wall defect repair.
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http://dx.doi.org/10.1039/c8bm01198bDOI Listing
March 2019

Preventive effects of nerve growth factor against colistin-induced autophagy and apoptosis in PC12 cells.

Toxicol Mech Methods 2019 Mar 21;29(3):177-186. Epub 2018 Nov 21.

a College of Veterinary Medicine , Northeast Agricultural University , Harbin , China.

In this study, the preventive effects of NGF against colistin-induced autophagy and apoptosis in PC12 cells have been investigated. Fluorescence microscopy, real-time PCR, transmission electron microscopy (TEM), flow cytometery, and western blotting technique were used. The results showed that large amounts of autophagosomes and apoptotic markers were triggered by colistin. Consistently, a significant increase has been noted at mRNA and protein levels in autophagy and apoptosis-related genes. Besides, TEM analysis showed that autophagic vacuoles were obvious at 12 h, while nuclear chromatin condensation and edge accumulation were clearly seen at 24 h in colistin alone group. Importantly, the visual autophagy and apoptosis were markedly reduced with NGF treatment in a dose-dependent manner. Moreover, colistin-induced reduction in mitochondrial membrane potential was partly attenuated by NGF in a dose dependent manner. In summary, NGF ameliorated colistin-induced apoptosis and autophagy, and partially recovered MMP in PC12 cells.
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http://dx.doi.org/10.1080/15376516.2018.1534298DOI Listing
March 2019

Assembled anti-adhesion polypropylene mesh with self-fixable and degradable in situ mussel-inspired hydrogel coating for abdominal wall defect repair.

Biomater Sci 2018 Oct;6(11):3030-3041

State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Abdominal adhesion to polypropylene (PP) mesh remains one of the major complications in hernia repair. Thus, a challenge exists to endow PP mesh with powerful anti-adhesion properties in hernia repair. To investigate potential options, the assembled PP mesh was developed with effective anti-adhesion properties through an in situ coating of the mesh surface with self-fixable and biodegradable mussel-inspired hydrogels. Through mixing oxidized-carboxymethylcellulose functionalized with dopamine (OCMC-DA) with carboxymethylchitosan (CMCS), a layer of hydrogel (OCMC-DA/CMCS) can be formed in situ on the PP mesh without the addition of crosslinking agents; the dopamine then acts as an immobilization group to fix these hydrogels to the PP mesh and the tissue surface. In this way, the assembled PP mesh (OCMC-DA/CMCS/PP) was obtained. The properties of the OCMC-DA/CMCS hydrogels were optimized, and the OCMC-DA4/CMCS hydrogel was selected to construct the assembled PP mesh. The lap-shear test revealed that OCMC-DA4/CMCS has tissue-adhesive properties. In vitro cell tests proved the excellent biocompatibility of the hydrogel. An optimized bioabsorption time and significant anti-adhesion properties were demonstrated through an in vivo test with a rat model. The adhesion area and tenacity of the OCMC-DA4/CMCS/PP group were more than 80% lower than those of the native PP mesh group and created a slightly inflammatory reaction.
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http://dx.doi.org/10.1039/c8bm00824hDOI Listing
October 2018

Enhancement of quality retention of fruiting bodies by erythorbic acid treatment.

3 Biotech 2018 Jul 5;8(7):305. Epub 2018 Jul 5.

4National Engineering Research Center of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403 People's Republic of China.

In this paper, the effects of erythorbic acid (EA) treatment with different concentrations on the quality of fruiting bodies stored at 4 °C for 27 days were studied by determining the changes in moisture content, weight loss, browning, electrolyte leakage, malondialdehyde (MDA), and nutritional compounds. The activities of polyphenoloxidase (PPO), cellulase and other antioxidant enzymes including superoxide dismutase (SOD), catalase, and peroxidase (POD) were also measured. Results showed that 0.1% EA-treated fruiting body maintained lower weight loss (< 6.0%, w/w), electrolyte leakage (< 45.8%), MDA (< 4.17 µmol kg), and higher moisture content (> 90.7%, w/w). Lower activities of PPO (< 72.64 × 10 U kg) and cellulase (< 189.86 × 10 U kg) in 0.1% EA-treated samples were observed compared with the other treatments. As a stereoisomer of ascorbic acid (AA), EA also could enhance SOD and POD activities of fruiting bodies. Our findings were the first time to evaluate the effect of EA on maintaining quality in fruiting bodies, and proved that low concentrations of EA (especially 0.1% EA, w/v) treatments were beneficial to preserve fruiting body with even higher efficiency than AA treatment. This study paved a foundation for the enhancement of quality retention of fruiting bodies.
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http://dx.doi.org/10.1007/s13205-018-1305-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033772PMC
July 2018

A Dual Role of P53 in Regulating Colistin-Induced Autophagy in PC-12 Cells.

Front Pharmacol 2017 27;8:768. Epub 2017 Oct 27.

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

This study aimed to investigate the mechanism of p53 in regulating colistin-induced autophagy in PC-12 cells. Importantly, cells were treated with 125 μg/ml colistin for 12 and 24 h after transfection with p53 siRNA or recombinant plasmid. The hallmarks of autophagy and apoptosis were examined by real-time PCR and western blot, fluorescence/immunofluorescence microscopy, and electron microscopy. The results showed that silencing of p53 leads to down-regulation of Atg5 and beclin1 for 12 h while up-regulation at 24 h and up-regulation of p62 noted. The ratio of LC3-II/I and autophagic vacuoles were significantly increased at 24 h, but autophagy flux was blocked. The cleavage of caspase3 and PARP (poly ADP-ribose polymerase) were enhanced, while PC-12-sip53 cells exposed to 3-MA showed down-regulation of apoptosis. By contrast, the expression of autophagy-related genes and protein reduced in p53 overexpressing cells following a time dependent manner. Meanwhile, there was an increase in the expression of activated caspase3 and PARP, condensed and fragmented nuclei were evident. Conclusively, the data supported that silencing of p53 promotes impaired autophagy, which acts as a pro-apoptotic induction factor in PC-12 cells treated with colistin for 24 h, and overexpression of p53 inhibits autophagy and accelerates apoptosis. Hence, it has been suggested that p53 could not act as a neuro-protective target in colistin-induced neurotoxicity.
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http://dx.doi.org/10.3389/fphar.2017.00768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664992PMC
October 2017

Colistin-induced autophagy and apoptosis involves the JNK-Bcl2-Bax signaling pathway and JNK-p53-ROS positive feedback loop in PC-12 cells.

Chem Biol Interact 2017 Nov 24;277:62-73. Epub 2017 Aug 24.

College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, PR China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, PR China. Electronic address:

Our recent study demonstrated neurotoxicity of colistin-induced autophagy and apoptosis in PC-12 cells, and that autophagy reached peak level at 12 h. In this study, we scrutinized the role of JNK in colistin-induced neurotoxicity and demonstrated the relationship among JNK, p53 and ROS in colistin treated PC-12 cells. Colistin-induced autophagy and apoptosis by JNK inhibition/activation were examined by western blotting, electron microscopy, and immunofluorescence/fluorescence microscopy. The results indicated that colistin induced JNK activation reached peak level at 12 h, while the highest levels of p-Bcl2/Bcl2 were observed at 12 h and Bax/Bcl2 significantly increased in a time-dependent manner. In PC-12 cells, inhibition of JNK by SP600125 (JNK inhibitor) resulted in significantly lower levels of autophagy upon colistin treatment, depending on the expression levels of Beclin1, LC3-II, p62 degradation and reduction in the number of autophagic vacuoles. In contrast, anisomycin pretreatment PC-12 cells led to upregulated autophagy. Especially, the highest levels of Beclin1 and p-Bcl2/Bcl2 were observed at 6 h, and Bax/Bcl2, cleaved-caspase3 and cleaved-PARP significantly increased in a time-dependent manner. The results revealed that JNK activation mediated autophagy and apoptosis related to Beclin1-Bcl2 and Bax-Bcl2 complex in colistin-treated PC-12 cells. Silencing of p53 by siRNA before colistin treatment substantially reduced ROS production and transactivated JNK in PC-12 cells. Moreover, activation of JNK increased ROS generation in PC-12 cells. In conclusion, colistin-induced autophagy and apoptosis is correlated to JNK-Bcl2-Bax signaling pathway, and an interaction effect found between intracellular ROS level and JNK-p53 signaling pathway in apoptosis.
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http://dx.doi.org/10.1016/j.cbi.2017.08.011DOI Listing
November 2017

TLR2 mediates autophagy through ERK signaling pathway in Mycoplasma gallisepticum-infected RAW264.7 cells.

Mol Immunol 2017 07 4;87:161-170. Epub 2017 May 4.

College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, PR China. Electronic address:

Toll-like receptor 2 (TLR2) plays a crucial role in early innate immune response of host to various microorganisms. Mycoplasma gallisepticum (MG) is one of the major pathogen that can cause chronic respiratory diseases in chickens, but the molecular mechanism of MG infection still remained unclear. In this study, we examined the typical hallmarks of autophagy and multiple signaling pathways by western blot, immunofluorescence microscopy and electron microscopy. The results indicated that infection of mouse macrophage cell line RAW264.7 with MG activated autophagy and mitogen-activated protein kinases (MAPKs). Silencing of TLR2 by siRNA substantially down-regulated MG-triggered autophagy in macrophages, and markedly reduced MG-induced extracellular regulated protein kinase (ERK) in macrophages but did not down-regulate c-Jun N-terminal kinase (JNK) and p38. Importantly, in macrophages, inhibition of ERK by PD98059 (ERK inhibitor) also significantly attenuated the level of autophagy upon MG infection, and the simultaneous treatment of TLR2 siRNA and PD98059 showed a similar effect on MG-induced autophagy as compared with TLR2 siRNA treatment alone. These findings thus demonstrate that TLR2 may mediate MG-induced autophagy through ERK signaling pathway in macrophage.
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http://dx.doi.org/10.1016/j.molimm.2017.04.013DOI Listing
July 2017

Self-healing pH-sensitive poly[(methyl vinyl ether)-alt-(maleic acid)]-based supramolecular hydrogels formed by inclusion complexation between cyclodextrin and adamantane.

Mater Sci Eng C Mater Biol Appl 2017 Apr 11;73:357-365. Epub 2016 Dec 11.

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Sipailou 2, Nanjing 210096, China; Research Institute of Southeast University in Suzhou, Ren Ai Road 150, Suzhou Industrial Park, Suzhou 215123, China.

Self-healing materials are of interest for drug delivery, cell and gene therapy, tissue engineering, and other biomedical applications. In this work, on the base of biocompatible polymer poly(methyl vinyl ether-alt-maleic acid) (P(MVE-alt-MA)), host polymer β-cyclodextrin-grafted P(MVE-alt-MA) (P(MVE-alt-MA)-g-β-CD) and guest polymer adamantane-grafted P(MVE-alt-MA) (P(MVE-alt-MA)-g-Ad) were first prepared. Then through taking advantage of the traditional host-guest interaction of β-cyclodextrin and adamantane, a novel self-healing pH-sensitive physical P(MVE-alt-MA)-g-β-CD/P(MVE-alt-MA)-g-Ad supramolecular hydrogels were obtained after simply mixing the aqueous solution of host polymer and guest polymer. This kind of supramolecular hydrogels not only possess pH-sensitivity, but also possess the ability to repair themselves after being damaged.
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http://dx.doi.org/10.1016/j.msec.2016.12.039DOI Listing
April 2017

Poly(dopamine)-inspired surface functionalization of polypropylene tissue mesh for prevention of intra-peritoneal adhesion formation.

J Mater Chem B 2017 Jan 22;5(3):575-585. Epub 2016 Dec 22.

State Key Lab of Bioelectronics, Jiangsu Key Lab for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Polypropylene (PP), as one of the most common prosthetic materials, has been widely used in intra-peritoneal repair. However, its adhesion to viscera has severely limited its application. Therefore it is critical to improve the PP surface with an anti-adhesion property. In this work, based on dopamine-inspired chemistry, virgin PP (V-PP) mesh was first pretreated with O plasma, subsequently dipped in dopamine aqueous solution for 24 h, and then chitosan (CS) was grafted onto it. Finally the anti-adhesion mesh (O-PP/PDA/CS) was obtained. The formation procedure of a PDA/CS ad-layer was characterized by water contact angle measurements, ATR-FTIR, SEM, and XPS. The results show that a PDA/CS ad-layer could be coated on the PP surface efficiently. NIH/3T3 cells were first cultured on O-PP/PDA/CS meshes to evaluate the availability of anti-adhesion and biocompatibility in vitro, and then the efficacy of the PDA/CS-coating as a barrier for reducing postsurgical adhesions was evaluated using a rat abdominal wall defect model. Compared with the V-PP group, NIH/3T3 cells exhibited higher viability in the O-PP/PDA/CS groups as evaluated by the CCK-8 method. In addition, NIH/3T3 cells grow into round-shapes on the O-PP/PDA/CS surface. This indicates that the modification strategy can facilely lead to excellent properties of anti-adhesion. In vivo tests further indicate that O-PP/PDA/CS meshes were effective in reducing adhesion formation.
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http://dx.doi.org/10.1039/c6tb02667bDOI Listing
January 2017