Publications by authors named "Wangyang Song"

6 Publications

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Evolutionary and functional analyses demonstrate conserved ferroptosis protection by Arabidopsis GPXs in mammalian cells.

FASEB J 2021 Jun;35(6):e21550

Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China.

Species have evolved unique mechanisms to combat the effects of oxidative stress inside cells. A particularly devastating consequence of an unhindered oxidation of membrane lipids in the presence of iron results in cell death, known as ferroptosis. Hallmarks of ferroptosis, including peroxidation of polyunsaturated fatty acids, are conserved among animals and plants, however, early divergence of an ancestral mammalian GPX4 (mGPX4) has complicated our understanding of mechanistic similarities between species. To this end, we performed a comprehensive phylogenetic analysis and identified that orthologous Arabidopsis GPXs (AtGPXs) are more highly related to mGPX4 than mGPX4 is to other mammalian GPXs. This high degree of conservation suggested that experimental substitution may be possible. We, therefore, ectopically expressed AtGPX1-8 in ferroptosis-sensitive mouse fibroblasts. This substitution experiment revealed highest protection against ferroptosis induction by AtGPX5, as well as moderate protection by AtGPX2, -7, and -8. Further analysis of these cells revealed substantial abatement of lipid peroxidation in response to pharmacological challenge. The results suggest that the presence of ancestral GPX4 resulted in later functional divergence and specialization of GPXs in plants. The results also challenge a strict requirement for selenocysteine activity and suggest thioredoxin as a potent parallel antioxidant system in both plants and mammals.
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http://dx.doi.org/10.1096/fj.202000856RDOI Listing
June 2021

Evolutionary Analysis of Calcium-Dependent Protein Kinase in Five Species.

Plants (Basel) 2019 Dec 24;9(1). Epub 2019 Dec 24.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

Calcium-dependent protein kinase (CPK) is crucial in Ca signal transduction, and is a large gene family in plants. In our previous work, we reported CPKs were important for natural rubber biosynthesis. However, this gene family in other rubber producing plants has not been investigated. Here, we report the CPKs in five representative species, including three rubber-producing and two non-rubber species. A total of 34, 34, 40, 34 and 30 CPKs were identified from , , , and , respectively. All CPKs were classified into four individual groups (group I to IV). In addition, 10 , 11 , 20 , 13 n and 7 c duplicated paralogs were identified. Further evolutionary analysis showed that, compared to other subfamilies, the group III had been expanded in the species, especially in the rubber-producing species. Meanwhile, the in group III from species tend to expand with low calcium binding capacity. This study provides a systematical evolutionary investigation of the CPKs in five representative species, suggesting that the sub-family specific expansion of CPKs might be related to natural rubber producing.
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http://dx.doi.org/10.3390/plants9010032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020201PMC
December 2019

Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Reveals the Critical Role of in Delaying Dark-Induced Leaf Senescence.

Int J Mol Sci 2019 Dec 6;20(24). Epub 2019 Dec 6.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton gene that acts as a positive factor in cell growth. Investigations on () family genes and their multiple functions are limited. The present study identified eight family genes and performed bioinformatic analyses. Expression analyses of the tissue specificity and developmental feature of s displayed their diverse expression patterns. Interestingly, demonstrated the most rapid significant increase in expression after 1 h of light recovery from the dark. Additionally, the transgenic / lines overexpressing in the late-flowering mutant displayed a recovery to the normal phenotype of wild-type plants. Moreover, compared to the mutant, the / transgenic presented delayed leaf senescence that was induced by the dark, indicating increased sensitivity to hydrogen peroxide (HO) under normal conditions that might be caused by a reduction in ascorbic acid (AsA) and ascorbic acid/dehydroascorbate (AsA/DHA) ratio. The results suggested that are functionally diverse in plant development and play a critical role in light responsiveness. Our study serves as a foundation for understanding the gene family in cotton and elucidating the regulatory mechanism of in delaying dark-induced leaf senescence.
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http://dx.doi.org/10.3390/ijms20246167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940856PMC
December 2019

Comparative Proteomic Analysis of Molecular Differences between Leaves of Wild-Type Upland Cotton and Its - Mutant.

Molecules 2019 Oct 19;24(20). Epub 2019 Oct 19.

College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.

mutant () ovules of upland cotton have been used to investigate cotton fiber development for decades. However, the molecular differences of green tissues between and wild-type (WT) cotton were barely reported. Here, we found that gossypol content, the most important secondary metabolite of cotton leaves, was higher in L. cv Xuzhou-142 (Xu142) WT than in . Then, we performed comparative proteomic analysis of the leaves from Xu142 WT and its . A total of 4506 proteins were identified, of which 103 and 164 appeared to be WT- and -specific, respectively. In the 4239 common-expressed proteins, 80 and 74 were preferentially accumulated in WT and , respectively. Pathway enrichment analysis and protein-protein interaction network analysis of both variety-specific and differential abundant proteins showed that secondary metabolism and chloroplast-related pathways were significantly enriched. Quantitative real-time PCR confirmed that the expression levels of 12 out of 16 selected genes from representative pathways were consistent with their protein accumulation patterns. Further analyses showed that the content of chlorophyll a in WT, but not chlorophyll b, was significantly increased compared to . This work provides the leaf proteome profiles of Xu142 and its mutant, indicating the necessity of further investigation of molecular differences between WT and leaves.
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http://dx.doi.org/10.3390/molecules24203769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832260PMC
October 2019

, the Key Gene for Ascorbate Biosynthesis in , Involves in Cell Elongation Under Control of Ethylene.

Cells 2019 09 5;8(9). Epub 2019 Sep 5.

College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.

L-Ascorbate (Asc) plays important roles in cell growth and plant development, and its de novo biosynthesis was catalyzed by the first rate-limiting enzyme VTC1. However, the function and regulatory mechanism of involved in cell development is obscure in . Herein, the Asc content and AsA/DHA ratio were accumulated and closely linked with fiber development. The GhVTC1 encoded a typical VTC1 protein with functional conserved domains and expressed preferentially during fiber fast elongation stages. Functional complementary analysis of in the loss-of-function mutants indicated that 1 is genetically functional to rescue the defects of mutants to normal or wild type (WT). The significant shortened primary root in mutants was promoted to the regular length of WT by the ectopic expression of in the mutants. Additionally, expression was induced by ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and the promoter showed high activity and included two ethylene-responsive elements (ERE). Moreover, the 5'-truncted promoters containing the ERE exhibited increased activity by ACC treatment. Our results firstly report the cotton function in promoting cell elongation at the cellular level, and serve as a foundation for further understanding the regulatory mechanism of Asc-mediated cell growth via the ethylene signaling pathway.
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http://dx.doi.org/10.3390/cells8091039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769745PMC
September 2019

A Cotton () -Inositol-1-Phosphate Synthase () Gene Promotes Root Cell Elongation in .

Int J Mol Sci 2019 Mar 11;20(5). Epub 2019 Mar 11.

College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.

-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) plays important roles in plant growth and development, stress responses, and cellular signal transduction. genes were found preferably expressed during fiber cell initiation and early fast elongation in upland cotton (), however, current understanding of the function and regulatory mechanism of genes to involve in cotton fiber cell growth is limited. Here, by genome-wide analysis, we identified four genes anchoring onto four chromosomes in and analyzed their phylogenetic relationship, evolutionary dynamics, gene structure and motif distribution, which indicates that genes are highly conserved from prokaryotes to green plants, with further exon-intron structure analysis showing more diverse in plants. Of the four members, based on the significant accumulated expression of at the early stage of fiber fast elongating development, thereby, the was selected to investigate the function of participating in plant development and cell growth, with ectopic expression in the loss-of-function mutants. The results showed that is a functional gene to fully compensate the abnormal phenotypes of the deformed cotyledon, dwarfed plants, increased inflorescence branches, and reduced primary root lengths in mutants. Furthermore, shortened root cells were recovered and normal root cells were significantly promoted by ectopic expression of in mutant and wild-type plants respectively. These results serve as a foundation for understanding the family genes in cotton, and suggest that may function as a positive regulator for plant cell elongation.
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http://dx.doi.org/10.3390/ijms20051224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429088PMC
March 2019