Publications by authors named "Wang Luo"

76 Publications

Identification of a Four Cancer Stem Cell-related Gene Signature and Establishment of a Prognostic Nomogram Predicting Overall Survival of Pancreatic Adenocarcinoma.

Comb Chem High Throughput Screen 2022 Jan 20. Epub 2022 Jan 20.

Department of Hepatobiliary Surgery I, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China | Guangdong Provincial Clinical and Engineering Center of Digital Medicine, Guangzhou, China.

Background: Cancer stem cells (CSCs) are now being considered as the initial component in the development of pancreatic adenocarcinoma (PAAD). Our aim was to develop a CSCrelated signature to assess the prognosis of PAAD patients for the optimization of treatment.

Materials And Methods: Differentially expressed genes (DEGs) between pancreatic tumor and normal tissue in the Cancer Genome Atlas (TCGA) were screened out, and the weighted gene correlation network analysis (WGCNA) was employed to identify the CSC-related gene sets. Then, univariate, Lasso Cox regression analyses and multivariate Cox regression were applied to construct a prognostic signature using the CSC-related genes. Its prognostic performance was validated in TCGA and ICGC cohorts. Furthermore, Univariate and multivariate Cox regression analyses were used to identify independent prognostic factors in PAAD, and a prognostic nomogram was established.

Results: The Kaplan-Meier analysis, ROC curve and C-index indicated the good performance of the CSC-related signature at predicting overall survival (OS). Univariate Cox regression and multivariate Cox regression revealed that the CSC-related signature was an independent prognostic factor in PAAD. The nomogram was superior to the risk model and AJCC stage in predicting OS. In terms of mutation and tumor immunity, patients in the high-risk group had higher tumor mutation burden (TMB) scores than patients in the low-risk group, and the immune score and the ESTIMATE score were significantly lower in the high-risk group. Moreover, according to the results of principal component analysis (PCA) and Gene Set Enrichment Analysis (GSEA), the low-risk and high-risk groups displayed different stemness statuses based on the risk model.

Conclusion: Our study identified four CSC-related gene signatures and established a prognostic nomogram that reliably predicts OS in PAAD. The findings may support new ideas for screening therapeutic targets to inhibit stem characteristics and the development of PAAD.
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http://dx.doi.org/10.2174/1386207325666220113142212DOI Listing
January 2022

Gene expression patterns of sea urchins (Strongylocentrotus intermedius) exposed to different combinations of temperature and hypoxia.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Dec 16;41:100953. Epub 2021 Dec 16.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning 116023, PR China. Electronic address:

Strongylocentrotus intermedius is one of the most economically valuable sea urchin species in China, and its growth and survival are severely constrained by ocean warming and the hypoxia that often accompanies high water temperatures. To elucidate the molecular mechanisms of S. intermedius that regulate gene expression in response to multi-causal environmental stresses. We performed a de novo transcriptome analysis of coelomocyte from S. intermedius to heat (25 °C), hypoxia (2 mg/L), and the combined stress. We identified 35,635, 29,107, and 29,440 differentially expressed genes (DEGs) in S. intermedius cultured under high temperature, low oxygen, and combined stress, respectively. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses revealed that these DEGs mainly enriched the functional categories of "Protein processing in endoplasmic reticulum," and "Glutathione metabolism" by heat stress, such as HSP70, GSTO1, PDIA4. After hypoxic stress, "Notch signaling pathway" and metabolism-related pathways such as "Glycerolipid metabolism", "Pyruvate metabolism" were significantly enriched. Exposure to combined stress resulted in a two-factor additive effect at the transcriptome level and have a more extensive impact on the immune correlated pathways in S. intermedius than single stress, the expression of related immune genes (C3, C5, and AIFM2) were up-regulated. Quantitative real-time PCR (qRT-PCR) analysis of the expression of 18 DEGs confirmed the RNA-Seq results. Observations in the present study will improve the understanding of the molecular mechanism of S. intermedius in response to multi-causal environmental stress.
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http://dx.doi.org/10.1016/j.cbd.2021.100953DOI Listing
December 2021

Common Immune-Related Adverse Events of Immune Checkpoint Inhibitors in the Gastrointestinal System: A Study Based on the US Food and Drug Administration Adverse Event Reporting System.

Front Pharmacol 2021 29;12:720776. Epub 2021 Nov 29.

Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China.

Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment; however, immune-related adverse events (irAEs) in the gastrointestinal (GI) system commonly occur. In this study, data were obtained from the US Food and Drug Administration adverse event reporting system between July 2014 and December 2020. Colitis, hepatobiliary disorders, and pancreatitis were identified as irAEs in our study. Reporting odds ratio (ROR) with information components (IC) was adopted for disproportionate analysis. A total of 70,330 adverse events were reported during the selected period, 4,075 records of which were associated with ICIs. GI toxicities have been reportedly increased with ICI, with ROR of 17.2, 6.7, and 2.3 for colitis, hepatobiliary disorders, and pancreatitis, respectively. The risks of colitis, hepatobiliary disorders, and pancreatitis were higher with anti-CTLA-4 treatment than that with anti-PD-1 (ROR 2.6, 1.3, and 1.1, respectively) or anti-PD-L1 treatment (ROR 4.8, 1.3, and 1.3, respectively). Logistic analysis indicated that hepatobiliary disorders and pancreatitis more frequently occurred in female patients (adjusted odds ratio, 1.16 and 1.52; both < 0.05). Consistently, polytherapy was a strong risk factor for colitis (adjusted odds ratio 2.52, < 0.001), hepatobiliary disorders (adjusted odds ratio 2.50, < 0.001), and pancreatitis (adjusted odds ratio 2.29, < 0.001) according to multivariate logistic analysis. This pharmacovigilance analysis demonstrated an increased risk of all three GI irAEs associated with ICI therapies. The comparative analysis offered supportive insights on selecting GI irAEs for patients treated with ICIs.
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http://dx.doi.org/10.3389/fphar.2021.720776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667785PMC
November 2021

Relationship between lake salinity and the climatic gradient in northeastern China and its implications for studying climate change.

Sci Total Environ 2022 Jan 17;805:150403. Epub 2021 Sep 17.

Key Laboratory of Cenozoic Geology and Environment, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China.

The rising temperatures, increased evaporation, and altered precipitation patterns associated with global warming pose threats to aquatic ecosystems, especially the salinization of lake water and changes in the terrestrial carbon budget. We studied a series of samples of catchment soils, surface sediments, and sediment cores from 51 lakes and reservoirs covering an extensive climatic range in northeastern China. Measurements included salinity indices (electrical conductivity and pH) and other physicochemical parameters, including magnetic properties and color (chroma). The results indicate that the occurrence of salt minerals and the salinity of the lake sediments are dominated by the arid climatic conditions of the region. This enabled us to develop climatic transfer functions between salinity, precipitation and evaporation, with potential applications in paleoclimatic research. As carbonates are the dominant salts in most of the studied lakes and reservoirs, past salinity variations are likely reflected by changes in HCO and CO concentrations, which provides the opportunity to study the response of water-CO-carbonate interactions to climate change. Our findings emphasize the important role of alkaline lakes in carbon burial and carbon neutralization, in the context of ongoing global warming.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150403DOI Listing
January 2022

Review of clinical tumor ablation advance in Asia.

Int J Hyperthermia 2021 ;38(1):1639-1649

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China.

Tumor ablation has been widely applied in Asia, accounting for 44.65% of clinical studies worldwide. We reviewed 5853 clinical studies to provide insight on the advance of tumor ablation in Asia chronologically and geographically among different techniques and organs. Since 1998, tumor ablation application has dramatically evolved in Asia. All kinds of ablation techniques, including percutaneous ethanol injection (PEI), radiofrequency ablation (RFA), microwave ablation (MWA), laser ablation (LA), cryoablation (CA), high-intensity focused ultrasound (HIFU), and irreversible electroporation (IRE), have been applied, with the first application of PEI and the most popular application of RFA. Twenty-five countries and one district in Asia have applied tumor ablation in various organs, including liver, lung, uterus, thyroid, kidney, pancreas, bone, prostate, breast, adrenal gland, lymph node parathyroid, esophagus, etc. Due to the high incidence of tumors as well as advanced economy and technology, East Asia accounted for 93.87% of studies, led by China (45.00%), Japan (32.72%), South Korea (12.10%), and Taiwan (4.03%). With the enrichment of evidence from large-scale multicenter and randomized control studies, China and South Korea have issued several guidelines on tumor ablation for liver, lung, and thyroid, which provided recommendations for global standardization of tumor ablation techniques. Therefore, Asia has made active contribution to global tumor ablation therapy.KeypointsKey point 1: Asia accounted for 44.65% of clinical studies worldwide on tumor ablation.Key point 2: Twenty-five countries and one district in Asia have used tumor ablation in various organs, and East Asia accounted for 93.87% of studies, led by China (45.00%), Japan (32.72%), South Korea (12.10%), and Taiwan (4.03%).Key point 3: China and South Korea have issued several guidelines on tumor ablation for liver, lung, and thyroid, which provided recommendations for global standardization of tumor ablation techniques.
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http://dx.doi.org/10.1080/02656736.2021.1983037DOI Listing
December 2021

ASO Visual Abstract: Laparoscopic in Situ Anatomical Mesohepatectomy for Solitary Massive HCC Using Combined Intrafascial and Extrafascial Approaches with Indocyanine Green Navigation (with Video).

Ann Surg Oncol 2021 Oct 25. Epub 2021 Oct 25.

Department of Hepatobiliary Surgery I, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

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http://dx.doi.org/10.1245/s10434-021-10968-1DOI Listing
October 2021

Laparoscopic in Situ Anatomical Mesohepatectomy for Solitary Massive HCC Using Combined Intrafascial and Extrafascial Approaches With Indocyanine Green Navigation (with Video).

Ann Surg Oncol 2021 Oct 13. Epub 2021 Oct 13.

Department of Hepatobiliary Surgery I, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: Laparoscopic anatomic mesohepatectomy for patients with hepatocellular carcinoma (HCC) remains technically challenging, especially for those with a massive tumor larger than 10 cm.

Methods: In this study, a 65-year-old man with a 13 × 10-cm solitary liver tumor located at segments 4, 5, and 8 underwent laparoscopic mesohepatectomy. To reduce the possibility of releasing cancer cells from the primary tumor, the in situ resection strategy for tumor removal was implemented. The intrafascial approach was used to dissect the right Glissonean pedicle, to transect the right anterior hepatic artery, and to ligate the right anterior portal vein. The extrafascial and transfissural approach was performed along the umbilical fissure to transect the Glissonean pedicle of segment 4. Indocyanine green (ICG) then was applied using "reverse staining" to visualize the resection extent and the right posterior hepatic duct (RPHD). During parenchymal resection, the right anterior Glissonean pedicle was adequately exposed and transected via the extrafascial approach above the plane of the RPHD. Finally, the right coronary ligament was dissected, and the tumor was removed.

Results: The operation was completed in 360 min, with a blood loss of 200 mL. The histopathologic diagnosis indicated a moderately differentiated HCC. The patient was discharged on postoperative day 8 without any complications.

Conclusion: Laparoscopic in situ anatomic mesohepatectomy using combined intra- and extrafascial approaches with ICG navigation may be feasible for patients with a centrally located solitary massive HCC.
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http://dx.doi.org/10.1245/s10434-021-10886-2DOI Listing
October 2021

DNAzyme based three-way junction assay for antibody-free detection of locus-specific N-methyladenosine modifications.

Biosens Bioelectron 2021 Dec 11;194:113625. Epub 2021 Sep 11.

Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical Laboratory Microfluidics and SPRi Engineering Research Center, Chongqing Medical University, No. 1 Yi Xue Yuan Road, Chongqing, 400016, PR China. Electronic address:

N-methyladenosine (m6A) is the most abundant post-transcriptional modification in RNA and has important implications in physiological processes and tumor development. However, sensitive and specific quantification of locus-specific m6A modification levels remains a challenging task. In the present work, a novel m6A-sensitive DNAzyme was utilized to directly detect m6A by coupling with a three-way junction-mediated isothermal exponential CRISPR amplification reaction for the first time. This method was built on the fact that the binding arm of the DNAzyme bound to the specific site and its core structure catalyzed the selective cleavage of unmodified adenine instead of methylated adenines. Subsequently, the intact RNA was identified by the proximity effect of the three-way junction. Enormous amounts of single-stranded DNA products were generated through a combination of SDA and EXPAR for signal amplification. The specific real-time curve of products was recorded through detecting the fluorescence intensity triggered by CRISPR Cas12a. As a result, methylation target of abundance down to 1% was successfully identified. In addition, this strategy could be used for the analysis of cell RNA extracts. Combined with an electrochemical sensor for quantitative detection of RNA methylation, we demonstrated the generality of as-proposed strategy. We envision the present method would provide a new platform for the analysis of m6A in RNA and promote its application in clinical diseases.
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http://dx.doi.org/10.1016/j.bios.2021.113625DOI Listing
December 2021

The Influence of Corticosteroids, Immunosuppressants and Biologics on Patients With Inflammatory Bowel Diseases, Psoriasis and Rheumatic Diseases in the Era of COVID-19: A Review of Current Evidence.

Front Immunol 2021 8;12:677957. Epub 2021 Jul 8.

Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China.

Patients with inflammatory bowel disease, psoriasis or other rheumatic diseases treated with corticosteroids, immunomodulators and biologics might face additional risk during COVID-19 epidemic due to their immunocompromised status. However, there was still no unanimous opinion on the use of these therapy during COVID-19 epidemic. Current studies suggested that systemic corticosteroids might increase the risk of hospitalization, as well as risks of ventilation, ICU, and death among patients with immune-mediated inflammatory diseases. Anti-TNF agent was associated with lower rate of hospitalization, as well as lower risks of ventilation, ICU, and death. No significant changes in rates of hospitalization, ventilation, ICU and mortality were observed in patients treated with immunomodulators or biologics apart from anti-TNF agents. The underlying mechanism of these results might be related to pathway of antiviral immune response and cytokine storm induced by SARS-COV-2 infection. Decision on the use of corticosteroids, immunomodulators and biologics should be made after weighing the benefits and potential risks based on individual patients.
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http://dx.doi.org/10.3389/fimmu.2021.677957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317986PMC
August 2021

Microplastic characteristics in organisms of different trophic levels from Liaohe Estuary, China.

Sci Total Environ 2021 May 26;789:148027. Epub 2021 May 26.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China. Electronic address:

Microplastics are a growing concern globally due to their small size and easy ingestion by terrestrial and aquatic organisms, resulting in potential adverse impacts on wildlife. However, current data regarding microplastics in wild organisms in different trophic levels is limited. This study investigated microplastic characteristics, including their abundance, size, shape and polymer type, in estuarine invertebrates and vertebrates. Resultantly, polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET) were the predominant microplastics found, as confirmed by a Fourier transform infrared spectrometer (FT-IR). An average microplastic abundance of 0.83 ± 0.99 to 3.87 ± 2.18 items/individual was detected across all species, including sandworm, mollusks, crustacean and fish, but they were not found in all individuals. Microplastics ranged from 52 μm to 5392 μm in size, and the shapes were consisted of fiber, fragment, and pellet. Moreover, the detection ratio (91.95%) and abundance (3.34 ± 2.17 items/individual) of microplastics in fish were significantly higher than in sandworm (42.86%, 0.88 ± 1.04 items/individual), mollusks (66.97%, 1.42 ± 1.41 items/individual) and crustaceans (66.66%, 1.33 ± 1.32 items/individual) (p < 0.05). Furthermore, a positive relationship was identified between microplastic abundance and the trophic level of organisms. These findings imply that microplastics might transfer along the food chain and accumulate at organisms in higher trophic levels.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148027DOI Listing
May 2021

The first complete chloroplast genome of (Diels) Hedberg (Caryophyllales: Polygonaceae) with phylogenetic implications.

Mitochondrial DNA B Resour 2021 5;6(8):2203-2205. Epub 2021 Jul 5.

Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industralization, School of Food and Biological Engineering, Chengdu University, Chengdu, P. R. China.

In the present study, we sequenced and assembled the complete chloroplast genome of (Diels) Hedberg. The chloroplast genome of was composed of 85 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. The chloroplast genome is 159,375 bp in length, with a GC content of 37.81%. Phylogenetic analysis based on the combined chloroplast gene dataset indicated that the exhibited a close relationship with .
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http://dx.doi.org/10.1080/23802359.2021.1945967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259871PMC
July 2021

An optimized free induction decay signal sensing coil and its matching circuit for miniaturized Overhauser geomagnetic sensor.

Rev Sci Instrum 2021 May;92(5):055005

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214035, China.

An Overhauser geomagnetic sensor is a precise instrument commonly employed for geomagnetic field observation, magnetic surveys, and so on. Currently, the miniaturization of the Overhauser geomagnetic sensor is limited due to the lower signal-to-noise ratio. Thus, how to effectively extract weaker free induction decay (FID) signal from a miniaturized sensor and how to improve the signal quality have become the bottleneck. To address these problems, we came up with an optimal design of the FID signal sensing coil for a miniaturized Overhauser geomagnetic sensor and propose a front-end matching circuit for the sensing coil to inhibit the attenuation of the signal amplitude caused by high impedance, further reducing the overall noise floor of the signal acquisition system. Finally, the field experimental results show that the miniaturized prototype sensor has a smaller volume and mass with an approximate performance compared with the commercial sensor.
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http://dx.doi.org/10.1063/5.0032431DOI Listing
May 2021

Comprehensive analysis of an immune-related ceRNA network in identifying a novel lncRNA signature as a prognostic biomarker for hepatocellular carcinoma.

Aging (Albany NY) 2021 07 8;13(13):17607-17628. Epub 2021 Jul 8.

Department of Hepatobiliary Surgery I, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

The function of competitive endogenous RNA (ceRNA) network in the immune regulation of hepatocellular carcinoma (HCC) is unclear. Our study aimed to construct an immune-related ceRNA network and develop an immune-related long noncoding RNA (lncRNA) signature to assess the prognosis of HCC patients and to optimize the treatment methods. We firstly constructed a ceRNA regulatory network for HCC using differentially expressed lncRNAs, mRNAs and microRNAs (miRNAs) from the Cancer Genome Atlas. A signature was constructed by 11 immune-related prognostic lncRNAs from the ceRNA network. The survival analysis and receiver operating characteristic analysis validated the reliability of the signature. Multivariate Cox regression analysis revealed that the signature could act an independent prognostic indicator. This signature also showed high association with immune cell infiltration and immune check blockades. LINC00491 was identified as the hub lncRNA in the signature. and evidence demonstrated that silencing of LINC00491 significantly inhibited HCC growth. Finally, 59 lncRNAs, 21 miRNAs, and 26 mRNAs were obtained to build the immune-related ceRNA network for HCC. In conclusion, our novel immune-related lncRNA prognostic signature and the immune-related ceRNA network might provide in-depth insights into tumor-immune interaction of HCC and promote better individual treatment strategies in HCC patients.
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http://dx.doi.org/10.18632/aging.203250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312417PMC
July 2021

Laparoscopic anatomic combined subsegmentectomy of segment 8 via the tailored strategy using digital intelligent technology.

Surg Oncol 2021 Sep 11;38:101622. Epub 2021 Jun 11.

The First Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China; Guangdong Provincial Clinical and Engineering Center of Digital Medicine, Guangzhou, 510282, China. Electronic address:

Introduction: Segment 8 is considered the largest liver segment, and its portal vein branches are generally divided into four parts, including ventral, dorsal, dorsolateral and medial branches (Shindoh et al., 2010; Takayasu et al., 1985) [1,2]. An anatomic combined subsegmentectomy could satisfy both the oncological quality of anatomical resection and the safety of parenchyma sparing principle if a small hepatocellular carcinoma is located between the hepatic subsegments (Berardi et al., 2021) [3]. Yet, laparoscopic anatomic combined subsegmentectomy of segment 8 is still technically challenging. The development of digital intelligent technology has made it possible to tailored preoperative planning and accurate intraoperative navigation in laparoscopic surgery.

Video: A 57-year-old man underwent a routine CT scan and was found to have a mass occupation in segment 8 of the liver. Three-dimensional reconstruction was performed to evaluate liver anatomy, vascular variations, and volume of each vascular unit as well as the location of the tumor, its relationship with the liver anatomy, and the Glissonian pedicles feeding the tumor-bearing area. Based on the reconstructed model, resection was planned aiming to the narrowest but oncologically safe anatomical tumor-bearing area. Upon evaluation, anatomic combined subsegmentectomy of segment 8 (ventral and medial subsegments) was confirmed. The operation was performed precisely under assistance of the Laparoscopic Hepatectomy Navigation System (LHNS, software copyright No. 2018SR840555) (Yang et al., 2020) [4].

Results: The operation lasted 200 min with 50 ml intraoperative blood loss. There were no postoperative complications, and the patient was discharged after 6 days.

Conclusion: Digital intelligent technology could provide tailored strategy for laparoscopic liver surgery, which makes laparoscopic anatomic combined subsegmentectomy of segment 8 feasible and effective.
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http://dx.doi.org/10.1016/j.suronc.2021.101622DOI Listing
September 2021

[COVID-19 Vaccination for Cancer Patients: Progress and Preliminary Recommendations].

Zhongguo Fei Ai Za Zhi 2021 Jun 24;24(6):377-383. Epub 2021 May 24.

Department of Respiratory and Critical Care Medicine, Peking Union Medical College 
Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

The pandemic of coronavirus disease 2019 (COVID-19) has had a serious impact on global health. COVID-19 vaccines may be one of the most effective measure to end the pandemic. High infection risk and higher serious incident and mortality rates have been shown in cancer patients with COVID-19. Therefore, cancer patients should be the priority group for COVID-19 prevention. Until now, data of COVID-19 vaccination for cancer patients is lacking. We review the interim data of safety and immune-efficacy of COVID-19 vaccination in cancer patients based on the latest studies. Due to the complicated immune systems of cancer patients caused by the malignancy and anticancer treatments, we proposed preliminary specific COVID-19 vaccination recommendations for cancer patients with different anticancer treatments and at different stages of the disease. Preventing COVID-19 with vaccinations for cancer patients is crucial, and we call for more large-scale clinical trials and real-world studies, for further COVID-19 vaccination recommendations development.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2021.101.18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246390PMC
June 2021

Effects of nursing based on Orem's self-care model on self-care efficacy, quality of life and adverse emotions in patients with advanced lung cancer.

Am J Transl Res 2021 15;13(4):2983-2989. Epub 2021 Apr 15.

Respiratory Medicine Department, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province, China.

Objective: To investigate the effects of nursing based on Orem's self-care model on self-care efficacy, quality of life (QOL) and adverse emotions of patients with advanced lung cancer (ALC) receiving chemotherapy.

Methods: A total of 71 patients with ALC aged 50-70 years, from our hospital were selected as the study subjects and divided into the control group (CNG, n = 35) and the experimental group (EXG, n = 36) using the random number table method. The CNG was treated with conventional chemotherapy combined with conventional nursing, while the EXG was treated with conventional chemotherapy combined with nursing based on Orem's self-care model. The effects on self-care efficacy, QOL and adverse emotions in the two groups were observed before and after nursing. The General Self-Efficacy Scale (GSES) was scored in both groups. The patients' body, physiology, psychology, society and health were scored using the QOL questionnaire for Chinese cancer patients receiving chemotherapy (QLQ-CCC). The self-rating anxiety scale (SAS) and self-rating depression scale (SDS) in the two groups were scored using the Hamilton anxiety scale (HAMA) and Hamilton depression scale (HAMD).

Results: The GSES scores in the EXG were remarkably higher than those in the CNG after intervention ( < 0.05). After intervention, the scores of the patients' body, physiology, psychology, society and health in the EXG were higher than those in the CNG ( < 0.05). The scores of SAS and SDS in the EXG were lower than those in the CNG ( < 0.05).

Conclusion: Nursing based on Orem's self-care model can effectively improve the self-care efficacy and QOL, adverse emotions (e.g., anxiety and depression), and degree of pain of patients with ALC receiving chemotherapy. Therefore, it has a positive clinical significance.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129333PMC
April 2021

Application of Real-Time Augmented Reality Laparoscopic Navigation in Splenectomy for Massive Splenomegaly.

World J Surg 2021 07 26;45(7):2108-2115. Epub 2021 Mar 26.

Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.

Objectives: To evaluate the clinical impact and technical feasibility of augmented reality laparoscopic navigation (ARLN) system in laparoscopic splenectomy for massive splenomegaly.

Methods: The clinical data of 17 consecutive patients who underwent laparoscopic splenectomy using ARLN (ARLN group) and 26 patients without ARLN guidance (Non-ARLN group) between January 2018 and April 2020 were enrolled. Propensity score matching (PSM) analysis was performed between the patients with and without ARLN guidance at a ratio of 1:1.

Results: Mean intraoperative blood loss was significantly lower in the ARLN-group than in the Non-ARLN group (306.6 ml vs. 462.6 ml, p = 0.047). All the patients in the ARLN-group achieved successful splenic artery dissection, while surgical success was achieved in 12 patients in the Non-ARLN group (p = 0.044). Postoperative hospital stay was significantly longer in the Non-ARLN group (3.8 days vs. 4.5 days, p = 0.040).

Conclusions: ARLN can provide feasible and accurate intraoperative image guidance, and it could be helpful in the performance of laparoscopic splenectomy for massive splenomegaly.
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http://dx.doi.org/10.1007/s00268-021-06082-8DOI Listing
July 2021

Modeling and reduction of the gradient effect of a magnetic sensor for an aeromagnetic compensator.

Rev Sci Instrum 2021 Feb;92(2):024502

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214000, China.

The traditional aeromagnetic compensation model does not consider the negative effects of the gradient of the geomagnetic background field. Especially in emergency aeromagnetic surveys, this gradient effect is obvious and can reduce the performance of magnetic compensation. To solve this problem, we propose a method of reducing the gradient effect of the magnetic sensor for aeromagnetic compensation. A magnetic gradient model of the Earth's background field is established for the aeromagnetic compensation, and the gradient effect is then suppressed using altitude data. An experimental platform was built, and flight tests were conducted. Comparison results show that the adoption of the proposed method increased the improvement ratio (IR) of our compensator on two flights from 20.4 and 21.3 to 25.9 and 26.0, with the growth rates of the IR, respectively, reaching 27% and 22%.
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http://dx.doi.org/10.1063/5.0028025DOI Listing
February 2021

Seasonal drought events in tropical East Asia over the last 60,000 y.

Proc Natl Acad Sci U S A 2020 12 23;117(49):30988-30992. Epub 2020 Nov 23.

Key Laboratory of Cenozoic Geology and Environment, Institute of Geology and Geophysics, Chinese Academy of Sciences, 100029, Beijing, China.

The cause of seasonal hydrologic changes in tropical East Asia during interstadial/stadial oscillations of the last glaciation remains controversial. Here, we show seven seasonal drought events that occurred during the relatively warm interstadials by phytolith and pollen records. These events are significantly manifested as high percentages of bilobate phytoliths and are consistent with the large zonal sea-surface temperature (SST) gradient from the western to eastern tropical Pacific, suggesting that the reduction in seasonal precipitation could be interpreted by westward shifts of the western Pacific subtropical high triggered by changes of zonal SST gradient over the tropical Pacific and Hadley circulation in the Northern Hemisphere. Our findings highlight that both zonal and meridional ocean-atmosphere circulations, rather than solely the Intertropical Convergence Zone or El Niño-Southern Oscillation, controlled the hydrologic changes in tropical East Asia during the last glaciation.
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http://dx.doi.org/10.1073/pnas.2013802117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733837PMC
December 2020

A novel method of fluorescent imaging can guide hepatectomy for intrahepatic cholangiocarcinoma with intrahepatic biliary obstruction.

J Surg Oncol 2020 Dec 7;122(8):1580-1586. Epub 2020 Sep 7.

Guangdong Provincial Clinical and Engineering Center of Digital Medicine, Guangzhou, China.

Objective: The aim of this study was to present a novel bile-duct obstructed area imaging (BOAI) and to investigate the feasibility and accuracy of this method in guiding hepatectomy for intrahepatic cholangiocarcinoma (ICC) with intrahepatic biliary obstruction.

Methods: From May 2017 to October 2019, eligible patients who underwent hepatectomy guided by BOAI were enrolled. Perioperative outcomes and operative data were analyzed. To assess the feasibility of BOAI and Glissonean pedicle approach, demarcations based on them were compared. To verify the accuracy of BOAI staining of the target territory, simple linear regression analysis, and intraclass correlation coefficient were used to examine the relationship between predicted resected liver volume (PRLV) and actual resected liver volume (ARLV).

Results: BOAI staining achieved valid demarcation in 15 (93.8%) of 16 patients, whereas the ischemic line achieved valid demarcation in only nine patients (57.3%; p = .017). ARLV and PRLV had a strong positive correlation (PRLV = 60.06 + 0.925 × ARLV; R = .945; p = .000). Meanwhile, ARLV (intraclass correlation coefficient = .971) achieved an excellent agreement with PRLV (p < .001).

Conclusions: The novel BOAI staining method can provide valid, feasible, and accurate demarcation line and may be an effective method in the surgical treatment of intrahepatic biliary obstruction.
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http://dx.doi.org/10.1002/jso.26204DOI Listing
December 2020

Fabrication of Micro-Structured LED Diffusion Plate Using Efficient Micro Injection Molding and Micro-Ground Mold Core.

Polymers (Basel) 2020 Jun 8;12(6). Epub 2020 Jun 8.

Guangdong Key Laboratory for Processing and Forming of Advanced Metallic Materials, School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou 510640, China.

In this paper, a new style of micro-structured LED (light-emitting diode) diffusion plate was developed using a highly efficient and precise hybrid processing method combined with micro injection molding and micro-grinding technology to realize mass production and low-cost manufacturing of LED lamps with excellent lighting performance. Firstly, the micro-structured mold core with controllable shape accuracy and surface quality was machined by the precision trued V-tip grinding wheel. Then, the micro-structured LED diffusion plate was rapidly fabricated by the micro injection molding technology. Finally, the influences of micro injection molding process parameters on the illumination of the micro-structured diffusion plate were investigated. The simulated optical results show that the illumination of the micro-structured diffusion plate can achieve a maximum value when the V-groove depth and V-groove angle are designed to be 300 μm and 60°, respectively. The experimental results indicate that the developed micro-structured diffusion plate may improve the illumination by about 40.82% compared with the traditional diffusion plate. The prediction accuracy of the designed light efficiency simulation method was about 90.33%.
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http://dx.doi.org/10.3390/polym12061307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7361868PMC
June 2020

Prevalence of Brugada Syndrome in a Large Population of Young Singaporean Men.

Circulation 2020 01 13;141(2):155-157. Epub 2020 Jan 13.

Medical Classification Centre, Central Manpower Base (X.S., B.Y.Q.T., C.H.S., J.S.W.L., M.D., K.W., D.Y.Z.L., G.G.R.S., E.C.Y.L., C.K.W.K., T.J.Y., D.T.T.C.), Singapore Armed Forces.

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http://dx.doi.org/10.1161/CIRCULATIONAHA.119.043670DOI Listing
January 2020

Suppression of the negative effect of abnormal data based on the Hough transform and application to the magnetic compensation of airborne optically-pumped magnetometer data.

Rev Sci Instrum 2019 Nov;90(11):115103

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214035, China.

A magnetic sensor of the optically pumped magnetometer may enter a dead zone during an aeromagnetic survey, resulting in unavoidable abnormal data and seriously affecting the effect of aeromagnetic compensation. We propose a fast discrimination and culling method based on the Hough transform to prevent abnormal data from participating in the coefficient estimation. In the proposed method, the parameter space is partitioned into small buckets and the most frequently passed region of sinusoidal curves is detected to eliminate abnormal data. Although the conventional method performs similarly to the proposed method when there is only 1% abnormal data, it is theoretically shown that the proposed method has a better goodness of fit of 0.9518, compared with a value of 0.1956 for the conventional method, in the presence of 45% abnormal data. Furthermore, we construct an experimental platform and conduct a flight test in which the proposed method has an improvement ratio of 4.11 compared with a value of 0.34 for the conventional method.
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http://dx.doi.org/10.1063/1.5121771DOI Listing
November 2019

Efficient and Precise Grinding of Sapphire Glass Based on Dry Electrical Discharge Dressed Coarse Diamond Grinding Wheel.

Micromachines (Basel) 2019 Sep 19;10(9). Epub 2019 Sep 19.

School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou 510640, China.

In this paper, in view of low grinding efficiency and poor ground surface quality of sapphire glass, the coarse diamond grinding wheel dressed by dry impulse electrical discharge was proposed to perform efficient and precise grinding machining of sapphire glass. The dry electrical discharge dressing technology was employed to obtain high grain protrusion and sharp micro-grain cutting edges. The influences of grinding process parameters such as wheel speed, depth of cut and feed speed on the ground surface quality, grinding force and grinding force ratio on sapphire glass were investigated, and the relationship between grinding force and ground surface quality was also revealed. The experimental results show that the grain protrusion height on the surface of a coarse diamond grinding wheel dressed by dry electrical discharge can reach 168.5 µm. The minimum line roughness and surface roughness of ground sapphire glass surface were 0.194 µm and 0.736 µm, respectively. In order to achieve highly efficient ground quality of sapphire glass, the depth of cut was controlled within 7 µm, and the wheel speed and feed speed were 3000-5000 r/min and 10-20 mm/min, respectively. The influences of feed speed and wheel speed on grinding force ratio were more significant, but the influence of depth of cut was little.
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http://dx.doi.org/10.3390/mi10090625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780427PMC
September 2019

CRM197-Coupled Der p 2 Peptides Suppress Allergic Airway Inflammation in a Der p 2-Induced Asthma Mouse Model.

Int Arch Allergy Immunol 2019 19;180(3):173-181. Epub 2019 Sep 19.

Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, China,

Allergic diseases affect more than 25% of the global population. Der p 2 is the major allergen of the house dust mite (HDM) Dermatophagoides pteronyssinus. Allergen-specific immunotherapy is the only treatment to change the course of allergic diseases. In this study, two synthesized Der p 2 peptides coupled to cross-reacting material 197 (CRM197) showed reduced IgE reactivity and allergenic activity. CRM197-coupled Der p 2 peptides induced rDer p 2-specific IgG1 antibodies in mice, which could inhibit HDM-allergic patients' IgE binding to rDer p 2. The immunity effects of CRM197-coupled Der p 2 peptides were studied in an rDer p 2-induced asthma mouse model. CRM197-coupled Der p 2 peptides can suppress asthmatic airway inflammation in this model. Analysis of IL-4, IL-5, and IFN-γ levels in bronchoalveolar lavage fluid revealed that the suppression was associated with a shift from a Th2 to a Th1 response. Thus, CRM197-bound Der p 2 peptides exhibited less allergenic activity than the rDer p 2 allergen, which preserved immunogenicity and may be candidates for mite allergy vaccines.
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http://dx.doi.org/10.1159/000502607DOI Listing
December 2019

Enhancing Sketch-Based Image Retrieval by CNN Semantic Re-ranking.

IEEE Trans Cybern 2020 Jul 15;50(7):3330-3342. Epub 2019 Mar 15.

This paper introduces a convolutional neural network (CNN) semantic re-ranking system to enhance the performance of sketch-based image retrieval (SBIR). Distinguished from the existing approaches, the proposed system can leverage category information brought by CNNs to support effective similarity measurement between the images. To achieve effective classification of query sketches and high-quality initial retrieval results, one CNN model is trained for classification of sketches, another for that of natural images. Through training dual CNN models, the semantic information of both the sketches and natural images is captured by deep learning. In order to measure the category similarity between images, a category similarity measurement method is proposed. Category information is then used for re-ranking. Re-ranking operation first infers the retrieval category of the query sketch and then uses the category similarity measurement to measure the category similarity between the query sketch and each initial retrieval result. Finally, the initial retrieval results are re-ranked. The experiments on different types of SBIR datasets demonstrate the effectiveness of the proposed re-ranking method. Comparisons with other re-ranking algorithms are also given to show the proposed method's superiority. Further, compared to the baseline systems, the proposed re-ranking approach achieves significantly higher precision in the top ten different SBIR methods and datasets.
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http://dx.doi.org/10.1109/TCYB.2019.2894498DOI Listing
July 2020

Radiofrequency ablation is safe and effective in the treatment of Chinese patients with gastroesophageal reflux disease: A single-center prospective study.

J Dig Dis 2019 May 21;20(5):229-234. Epub 2019 Apr 21.

Digestive Endoscopy Center, Department of Gastroenterology, Changhai Hospital, Second Military Medical University (Naval Medical University), Shanghai, China.

Objective: This study aimed to evaluate the safety and efficiency of radiofrequency ablation (RFA) in Chinese patients with gastroesophageal reflux disease (GERD).

Methods: This was a single-center, prospective study including 27 Chinese patients with GERD. The outcomes in all patients were evaluated before and at 3, 6, and 12 months after RFA, including their GERD health-related quality of life (GERD-HRQL) score, esophageal acid exposure, DeMeester score, lower esophageal sphincter (LES) resting pressure, and patient's satisfaction with symptom control. Furthermore, rabeprazole sodium (RS) administration, reflux esophagitis (RE), and intraoperative and postoperative complications were also evaluated.

Results: RFA treatment significantly reduced the GERD-HRQL score, the percentage of time that esophageal pH < 4, and the DeMeester score, and significantly increased the LES resting pressure in GERD patients. A need for RS administration was reduced and RE symptoms were relieved. Satisfaction rate of 92.6% and 96.3% was reported by these patients at 6 and 12 months post-treatment, respectively. Mild bleeding (<20 mL) occurred in one patient during RFA, and no serious intraoperative and postoperative complications were observed.

Conclusion: RFA is safe and effective in the treatment of GERD in Chinese patients.
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http://dx.doi.org/10.1111/1751-2980.12722DOI Listing
May 2019

Virtual Touch Tissue Imaging and Quantification (VTIQ) combined with the American College of Radiology Thyroid Imaging Reporting and Data System (ACR TI-RADS) for malignancy risk stratification of thyroid nodules.

Clin Hemorheol Microcirc 2019 ;72(3):279-291

In-Patient Ultrasound Department, Second Affiliated Hospital of Harbin Medical University, Nangang, Harbin, Heilongjiang, China.

Objective: This study was conducted to investigate the diagnostic performance of Virtual Touch Tissue Imaging and Quantification (VTIQ), combined with the American College of Radiology Thyroid Imaging Reporting and Data System (ACR TI-RADS) in differentiating malignant and benign thyroid nodules.

Methods: A total of 130 thyroid nodules in 128 patients were included. The diagnostic performance of conventional ultrasound (US), VTIQ, and the combination of these two techniques was calculated and compared according to the area under the receiver operating characteristic curve (AUROC), for sensitivity, specificity, and accuracy.

Results: The sensitivity and specificity for the ACR TI-RADS were 98.6% (72/73) and 24.6% (14/57), respectively. There was a strong agreement with ACR TI-RADS categories of thyroid nodules (all ICCs > 0.60). With an optimal cutoff value of 2.46 m/s, the sensitivity and specificity of the minimal shear wave velocity (SWVmin) were 87.7% (64/73) and 70.2% (40/57). By applying this value to downgrade or upgrade ACR TI-RADS, the specificity significantly increased from 24.6% (14/57) to 47.4% (27/57; P < 0.05) and the sensitivity remained at 98.6% (72/73).

Conclusions: VTIQ combined with ACR TI-RADS could improve the specificity of the differential diagnosis of thyroid nodules without a loss of sensitivity.
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http://dx.doi.org/10.3233/CH-180477DOI Listing
November 2019

The EGFR T790M Mutation Is Acquired through AICDA-Mediated Deamination of 5-Methylcytosine following TKI Treatment in Lung Cancer.

Cancer Res 2018 12 17;78(24):6728-6735. Epub 2018 Oct 17.

Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan.

: Almost all patients with EGFR-driven lung cancer who are treated with EGFR tyrosine kinase inhibitors (TKI) develop resistance to treatment. A single base (c.2369C>T) transition mutation, T790M, is the most frequent resistance event after first-generation exposure to EGFR TKIs. Whether T790M mutation is acquired or is selected from a preexisting clone has been a matter of significant debate. In this study, we show that treatment with EGFR TKIs leads to activation of the NFκB pathway, which in turn induces expression of activation-induced cytidine deaminase (AICDA). In turn, AICDA causes deamination of 5-methylcytosine to thymine at position c.2369 to generate the T790M mutation. Pharmacologic inhibition of the NFκB pathway or knockout of AICDA decreased the frequency or prevented the development of T790M mutation, respectively. In addition, patients treated with first-line EGFR TKI displayed increased expression of AICDA and detection of the T790M mutation upon progression. These results identify the mechanism of T790M acquisition and present an opportunity to target the process to delay or prevent it. SIGNIFICANCE: These findings identify the mechanism behind acquisition of a common resistance mutation to TKI treatment in lung cancer.
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http://dx.doi.org/10.1158/0008-5472.CAN-17-3370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295274PMC
December 2018

Learning Latent Stable Patterns for Image Understanding With Weak and Noisy Labels.

IEEE Trans Cybern 2019 Dec 5;49(12):4243-4252. Epub 2018 Oct 5.

This paper focuses on weakly supervised image understanding, in which the semantic labels are available only at image-level, without the specific object or scene location in an image. Existing algorithms implicitly assume that image-level labels are error-free, which might be too restrictive. In practice, image labels obtained from the pretrained predictors are easily contaminated. To solve this problem, we propose a novel algorithm for weakly supervised segmentation when only noisy image labels are available during training. More specifically, a semantic space is constructed first by encoding image labels through a graphlet (i.e., superpixel cluster) embedding process. Then, we observe that in the semantic space, the distribution of graphlets from images with a same label remains stable, regardless of the noises in image labels. Therefore, we propose a generative model, called latent stability analysis, to discover the stable patterns from images with noisy labels. Inferring graphlet semantics by making use of these mid-level stable patterns is much more secure and accurate than directly transferring noisy image-level labels into different regions. Finally, we calculate the semantics of each superpixel using maximum majority voting of its correlated graphlets. Comprehensive experimental results show that our algorithm performs impressively when the image labels are predicted by either the hand-crafted or deeply learned image descriptors.
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http://dx.doi.org/10.1109/TCYB.2018.2861419DOI Listing
December 2019
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