Publications by authors named "Wang Huan"

2,065 Publications

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MORC protein family-related signature within human disease and cancer.

Cell Death Dis 2021 Nov 27;12(12):1112. Epub 2021 Nov 27.

Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110122, China.

The microrchidia (MORC) family of proteins is a highly conserved nuclear protein superfamily, whose members contain common domain structures (GHKL-ATPase, CW-type zinc finger and coiled-coil domain) yet exhibit diverse biological functions. Despite the advancing research in previous decades, much of which focuses on their role as epigenetic regulators and in chromatin remodeling, relatively little is known about the role of MORCs in tumorigenesis and pathogenesis. MORCs were first identified as epigenetic regulators and chromatin remodelers in germ cell development. Currently, MORCs are regarded as disease genes that are involved in various human disorders and oncogenes in cancer progression and are expected to be the important biomarkers for diagnosis and treatment. A new paradigm of expanded MORC family function has raised questions regarding the regulation of MORCs and their biological role at the subcellular level. Here, we systematically review the progress of researching MORC members with respect to their domain architectures, diverse biological functions, and distribution characteristics and discuss the emerging roles of the aberrant expression or mutation of MORC family members in human disorders and cancer development. Furthermore, the illustration of related mechanisms of the MORC family has made MORCs promising targets for developing diagnostic tools and therapeutic treatments for human diseases, including cancers.
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http://dx.doi.org/10.1038/s41419-021-04393-1DOI Listing
November 2021

Genome-Wide Characterization of Salt-Responsive miRNAs, circRNAs and Associated ceRNA Networks in Tomatoes.

Int J Mol Sci 2021 Nov 12;22(22). Epub 2021 Nov 12.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Soil salinization is a major environmental stress that causes crop yield reductions worldwide. Therefore, the cultivation of salt-tolerant crops is an effective way to sustain crop yield. Tomatoes are one of the vegetable crops that are moderately sensitive to salt stress. Global market demand for tomatoes is huge and growing. In recent years, the mechanisms of salt tolerance in tomatoes have been extensively investigated; however, the molecular mechanism through which non-coding RNAs (ncRNAs) respond to salt stress is not well understood. In this study, we utilized small RNA sequencing and whole transcriptome sequencing technology to identify salt-responsive microRNAs (miRNAs), messenger RNAs (mRNAs), and circular RNAs (circRNAs) in roots of M82 cultivated tomato and () wild tomato under salt stress. Based on the theory of competitive endogenous RNA (ceRNA), we also established several salt-responsive ceRNA networks. The results showed that circRNAs could act as miRNA sponges in the regulation of target mRNAs of miRNAs, thus participating in the response to salt stress. This study provides insights into the mechanisms of salt tolerance in tomatoes and serves as an effective reference for improving the salt tolerance of salt-sensitive cultivars.
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http://dx.doi.org/10.3390/ijms222212238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625345PMC
November 2021

From natural products to HDAC inhibitors: An overview of drug discovery and design strategy.

Bioorg Med Chem 2021 Nov 10;52:116510. Epub 2021 Nov 10.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China. Electronic address:

Histone deacetylases (HDACs) play a key role in the homeostasis of protein acetylation in histones and have recently emerged as a therapeutic target for numerous diseases. The inhibition of HDACs may block angiogenesis, arrest cell growth, and lead to differentiation and apoptosis in tumour cells. Thus, HDAC inhibitors (HDACi) have received increasing attention and many of which are developed from natural sources. In the past few decades, naturally occurring HDACi have been identified to have potent anticancer activities, some of which have demonstrated promising therapeutic effects on haematological malignancies. In this review, we summarized the discovery and modification of HDAC inhibitors from natural sources, novel drug design that uses natural products as parent nuclei, and dual target design strategies that combine HDAC with non-HDAC targets.
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http://dx.doi.org/10.1016/j.bmc.2021.116510DOI Listing
November 2021

Serum and glucocorticoid-regulated kinase 1 regulates transforming growth factor β1-connective tissue growth factor pathway in chronic rhinosinusitis.

Clin Immunol 2021 Nov 23:108895. Epub 2021 Nov 23.

Department of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Fudan University, No. 83, Fenyang Road, Xuhui District, Shanghai, China. Electronic address:

Purpose: Serum/glucocorticoid-regulated kinase 1 (SGK1) has been identified as a crucial regulator in fibrotic disorders. Herein, we explored SGK1 role in tissue remodeling of chronic rhinosinusitis (CRS).

Methods: Lentivirus was employed to generate an SGK1-overexpressing human bronchial epithelial cell (16HBE) line. To screen SGK1 downstream genes, RNA sequencing was performed on SGK1-overexpressing and control cell lines. To determine protein and gene expression levels, immunohistochemistry, western blotting, and quantitative real-time polymerase chain reaction were employed. Correlation analysis was performed using mRNA expression levels of SGK1, transforming growth factor β1 (TGF-β1), and connective tissue growth factor (CTGF) derived from CRS mucosal tissue and GEO database. Gene set enrichment analysis was conducted using gene sets from Molecular Signatures Database. The severity of symptoms in CRS patients was assessed using the 22-Item Sinonasal Outcome Test.

Results: SGK1 overexpression significantly increased the expression of connective tissue growth factor (CTGF) in 16HBE cells (P < 0.01). Consistently, CTGF protein level was considerably greater in mucosal tissue of CRS without nasal polyps (CRSsNP) than in CRS with nasal polyps (CRSwNP) (P < 0.05) or in control subjects (P < 0.01). TGF-β1 protein level was higher in mucosal tissue of CRSsNP patients than in CRSwNP patients (P < 0.001) or in the control group (P < 0.01). mRNA levels of SGK1 and CTGF (P < 0.05, r = 0.668; P = 0.001, r = 0.630), TGF-β1 and CTGF (P < 0.05, r = 0.560; P < 0.05, r = 0.420), as well as SGK1 and TGF-β1(P < 0.05, r = 0.612; P < 0.05, r = 0.524) were significantly correlated in CRS mucosal tissue and GSE36830 dataset, respectively. TGF-β1-induced upregulated genes were significantly enriched in SGK1 overexpression group. In vitro assays, TGF-β1 promoted SGK1 and CTGF expression in a concentration- and time-dependent manner. Administrating an SGK1 inhibitor, GSK650394, significantly inhibited TGF-β1-induced CTGF expression in 16HBE and dispersed primary nasal polyp cells.

Conclusions: TGF-β1 stimulation significantly increases SGK1 and CTGF expression. By regulating TGF-β1-CTGF pathway, SGK1 may participate in tissue remodeling in the pathological mechanism of CRS.
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http://dx.doi.org/10.1016/j.clim.2021.108895DOI Listing
November 2021

Azaphilones as Activation-Free Primary-Amine-Specific Bioconjugation Reagents for Peptides, Proteins and Lipids.

Angew Chem Int Ed Engl 2021 Nov 26. Epub 2021 Nov 26.

Nanjing University, School of Chemistry and Chemical Engineering, 163 Xianlin Avenue, Chemistry Building E515, 210023, Nanjing, CHINA.

Residue-selective bioconjugation methods for biomolecules are highly sought to expand the scope of their biological and medical applications. Inspired by the generation mechanism of natural vinylogous γ -pyridones (vPDNs), we have developed a novel unique azaphilone-based, activation-free primary amine-selective bioconjugation method for biomolecules. Our strategy allows facile functionalization of primary amine groups in peptides and proteins, including the clinically used therapeutic antibody trastuzumab, by generating a highly stable vPDN linkage. Excellent chemoselectivity toward primary amines also enables the azaphilone derivatives to specifically modify the lipid components of Gram-positive bacteria while bypassing Gram-negative bacteria and mammalian cells. The new method shows significant advantages including chemoselectivity, efficiency, flexibility and biocompatibility, and therefore provides a valuable addition to the current toolbox for biomolecule conjugation.
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http://dx.doi.org/10.1002/anie.202111783DOI Listing
November 2021

The necessity of wearing facemasks correctly especially for students during the epidemic.

Disaster Med Public Health Prep 2021 Nov 26:1-7. Epub 2021 Nov 26.

Department of Acupuncture and Moxibustion, Tianjin Gong An Hospital, Tianjin, 300042, China.

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http://dx.doi.org/10.1017/dmp.2021.345DOI Listing
November 2021

Manganese-Catalyzed [4 + 2] Annulation of N-H Amidines with Vinylene Carbonate via C-H Activation.

J Org Chem 2021 Nov 25. Epub 2021 Nov 25.

State Key Laboratory for Oxo Synthesis and Selective Oxidation, Suzhou Research Institute of LICP, Center for Excellence in Molecular Synthesis, Lanzhou Institute of Chemical Physics (LICP), Chinese Academy of Sciences, Lanzhou730000, P. R. China.

Manganese-catalyzed C-H bond functionalization of aryl amidines for the synthesis of 1-aminoisoquinolines in the presence of vinylene carbonate has been developed. The reaction features a broad substrate scope and proceeds under mild reaction conditions with only the carbonate anion as the byproduct.
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http://dx.doi.org/10.1021/acs.joc.1c02473DOI Listing
November 2021

Multiple Stimuli-Responsive MXene-Based Hydrogel as Intelligent Drug Delivery Carriers for Deep Chronic Wound Healing.

Small 2021 Nov 25:e2104368. Epub 2021 Nov 25.

Department of Pharmaceutical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China.

Chronic wound healing is an important and basic issue in medical and healthcare fields. Recently, stimuli-responsive hydrogel systems have emerged as promising drug delivery carriers for wound management. However, given to the limited therapeutic outcomes, new hydrogel systems for efficient wound treatment are urgently needed. Here, the development of a 2D MXene-based hydrogel system for highly efficient photo- and magnetic-responsive drug delivery oriented to deep chronic wounds repair is presented. The intelligent responsive MXene-based hydrogel drug delivery system is composed of MXene-wrapped magnetic colloids and poly(N-isopropyl acrylamide)-alginate dual-network hydrogels. It is demonstrated that the MXene-based hydrogel system exhibits multiple response capability and controllable drug delivery ability, which can reduce the toxic side effects of drugs and promote the wound healing process as well. Notably, the practical performance of the MXene-based hydrogel drug delivery system is demonstrated by applying it to the treatment of the full-thickness cutaneous wound and subcutaneous infected wound of the rat model, which indicates the great prospect in clinical wound healing and other related biomedical fields.
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http://dx.doi.org/10.1002/smll.202104368DOI Listing
November 2021

Advances in the application of Let-7 microRNAs in the diagnosis, treatment and prognosis of leukemia.

Oncol Lett 2022 Jan 2;23(1). Epub 2021 Nov 2.

School of Life Science and Technology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.

The lethal-7 (Let-7) family of microRNAs (miRNAs) controls the process of development and differentiation, but is also related to the occurrence of tumors and a poor prognosis of patients with tumors. Thus, a more comprehensive exploration of its functions will provide further insights into these processes, and may promote the diagnosis and treatment of tumors. Leukemia is a type of progressive malignant disease, and its pathogenesis involves a variety of epigenetic factors. Amongst the several related epigenetic factors, the Let-7 miRNAs are an important family of molecules that play a crucial role in maintaining a variety of critical biological processes, including development, differentiation and proliferation. In the present study, the role of Let-7 as a tumor suppressor gene and oncogene is reviewed, and the complex regulatory functions of several Let-7 family members in different subtypes of leukemia are described. The current body of knowledge thus far indicates that Let-7 is not only a potential diagnostic and prognostic marker of leukemia, but also a potential therapeutic target for the treatment of affected patients, with particular potential when targeted by adjuvant treatments alongside traditional treatment to improve their survival rate.
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http://dx.doi.org/10.3892/ol.2021.13119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607238PMC
January 2022

Nanomotor-Derived Porous Biomedical Particles from Droplet Microfluidics.

Adv Sci (Weinh) 2021 Nov 23:e2104272. Epub 2021 Nov 23.

Department of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China.

Porous particles have found widespread applications in therapeutic diagnosis, drug delivery, and tissue engineering due to their typical properties of large surface area, extensive loading capacity, and hierarchical microstructures. Attempts in this aspect are focusing on the development of effective methods to generate functional porous particles. Herein, a simple droplet microfluidics for continuously and directly generating porous particles by introducing bubble-propelled nanomotors into the system is presented. As the nanomotors can continuously generate gas bubbles in the unsolidified droplet templates, the desirable porous microparticles can be obtained after droplet polymerization. It is demonstrated that the generation process is highly controlled and the resultant microparticles show excellent porosity and monodispersity. In addition, the obtained porous microparticles can serve as microcarriers for 3D cell culture, because of their characteristic porous structures and favorable biocompatibility. Moreover, owing to the existence of oxygen in these microparticles, they can be used to improve the healing effects of wounds in the type I diabetes rat models. These remarkable features of the generation strategy and the porous microparticles point to their potential values in various biomedical fields.
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http://dx.doi.org/10.1002/advs.202104272DOI Listing
November 2021

Enzyme Control Over Ferric Iron Magnetostructural Properties.

Angew Chem Int Ed Engl 2021 Nov 23. Epub 2021 Nov 23.

Massachusetts General Hospital and Harvard Medical School, Radiology, 149 Thirteenth Street, Suite 2301, 02129, Charlestown, UNITED STATES.

Fe 3+ complexes in aqueous solution can exist as discrete mononuclear species or multinuclear magnetically coupled species. Stimuli-driven change to Fe 3+ speciation represents a powerful mechanistic basis for magnetic resonance sensor technology, but ligand design strategies to exert precision control of aqueous Fe 3+ magnetostructural properties are entirely underexplored. In pursuit of this objective, we rationally designed a ligand to strongly favor a dinuclear µ-oxo-bridged and antiferromagnetically coupled complex, but which undergoes carboxylesterase mediated transformation to a mononuclear high-spin Fe 3+ chelate resulting in substantial T 1 -relaxivity increase. The data communicated demonstrate proof of concept for a novel and effective strategy to exert biochemical control over aqueous Fe 3+ magnetic, structural, and relaxometric properties.
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http://dx.doi.org/10.1002/anie.202114019DOI Listing
November 2021

Mild early-life stress exaggerates the impact of acute stress on corticolimbic resting-state functional connectivity.

Eur J Neurosci 2021 Nov 22. Epub 2021 Nov 22.

Donders Institute for Brain, Cognition and Behaviour, Radboud University and Radboud university medical center, HB, Nijmegen, The Netherlands.

Abundant evidence shows that early-life stress (ELS) predisposes for the development of stress-related psychopathology when exposed to stressors later in life, but the underlying mechanisms remain unclear. To study predisposing effects of mild ELS on stress sensitivity, we examined in a healthy human population the impact of a history of ELS on acute stress-related changes in corticolimbic circuits involved in emotional processing (i.e., amygdala, hippocampus, ventromedial prefrontal cortex; vmPFC). Healthy young male participants (n=120) underwent resting-state functional magnetic resonance imaging (fMRI) in two separate sessions (stress induction versus control). The Childhood Trauma Questionnaire (CTQ) was administered to index self-reported ELS, and stress induction was verified using salivary cortisol, blood pressure, heart rate, and subjective affect. Our findings show that self-reported ELS was negatively associated with baseline cortisol, but not with the acute stress-induced cortisol response. Critically, individuals with more self-reported ELS exhibited an exaggerated reduction of functional connectivity in corticolimbic circuits under acute stress. A mediation analysis showed that the association between ELS and stress-induced changes in amygdala-hippocampal connectivity became stronger when controlling for basal cortisol. Our findings show, in a healthy sample, that the effects of mild ELS on functioning of corticolimbic circuits only become apparent when exposed to an acute stressor, and may be buffered by adaptations in hypothalamic-pituitary-adrenal axis function. Overall, our findings might reveal a potential mechanism whereby even mild ELS might confer vulnerability to exposure to stressors later in adulthood.
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http://dx.doi.org/10.1111/ejn.15538DOI Listing
November 2021

Prediction Model of Osteonecrosis of the Femoral Head After Femoral Neck Fracture: Machine Learning-Based Development and Validation Study.

JMIR Med Inform 2021 Nov 19;9(11):e30079. Epub 2021 Nov 19.

Department of Medical Statistics, Tongji University School of Medicine, Shanghai, China.

Background: The absolute number of femoral neck fractures (FNFs) is increasing; however, the prediction of traumatic femoral head necrosis remains difficult. Machine learning algorithms have the potential to be superior to traditional prediction methods for the prediction of traumatic femoral head necrosis.

Objective: The aim of this study is to use machine learning to construct a model for the analysis of risk factors and prediction of osteonecrosis of the femoral head (ONFH) in patients with FNF after internal fixation.

Methods: We retrospectively collected preoperative, intraoperative, and postoperative clinical data of patients with FNF in 4 hospitals in Shanghai and followed up the patients for more than 2.5 years. A total of 259 patients with 43 variables were included in the study. The data were randomly divided into a training set (181/259, 69.8%) and a validation set (78/259, 30.1%). External data (n=376) were obtained from a retrospective cohort study of patients with FNF in 3 other hospitals. Least absolute shrinkage and selection operator regression and the support vector machine algorithm were used for variable selection. Logistic regression, random forest, support vector machine, and eXtreme Gradient Boosting (XGBoost) were used to develop the model on the training set. The validation set was used to tune the model hyperparameters to determine the final prediction model, and the external data were used to compare and evaluate the model performance. We compared the accuracy, discrimination, and calibration of the models to identify the best machine learning algorithm for predicting ONFH. Shapley additive explanations and local interpretable model-agnostic explanations were used to determine the interpretability of the black box model.

Results: A total of 11 variables were selected for the models. The XGBoost model performed best on the validation set and external data. The accuracy, sensitivity, and area under the receiver operating characteristic curve of the model on the validation set were 0.987, 0.929, and 0.992, respectively. The accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve of the model on the external data were 0.907, 0.807, 0.935, and 0.933, respectively, and the log-loss was 0.279. The calibration curve demonstrated good agreement between the predicted probability and actual risk. The interpretability of the features and individual predictions were realized using the Shapley additive explanations and local interpretable model-agnostic explanations algorithms. In addition, the XGBoost model was translated into a self-made web-based risk calculator to estimate an individual's probability of ONFH.

Conclusions: Machine learning performs well in predicting ONFH after internal fixation of FNF. The 6-variable XGBoost model predicted the risk of ONFH well and had good generalization ability on the external data, which can be used for the clinical prediction of ONFH after internal fixation of FNF.
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http://dx.doi.org/10.2196/30079DOI Listing
November 2021

Strategies for Developing Transition Metal Phosphides in Electrochemical Water Splitting.

Authors:
Jie Ying Huan Wang

Front Chem 2021 3;9:700020. Epub 2021 Nov 3.

School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai, China.

Electrochemical water splitting involving hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is a greatly promising technology to generate sustainable and renewable energy resources, which relies on the exploration regarding the design of electrocatalysts with high efficiency, high stability, and low cost. Transition metal phosphides (TMPs), as nonprecious metallic electrocatalysts, have been extensively investigated and proved to be high-efficient electrocatalysts in both HER and OER. In this minireview, a general overview of recent progress in developing high-performance TMP electrocatalysts for electrochemical water splitting has been presented. Design strategies including composition engineering by element doping, hybridization, and tuning the molar ratio, structure engineering by porous structures, nanoarray structures, and amorphous structures, and surface/interface engineering by tuning surface wetting states, facet control, and novel substrate are summarized. Key scientific problems and prospective research directions are also briefly discussed.
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http://dx.doi.org/10.3389/fchem.2021.700020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595924PMC
November 2021

2-Phenylcyclopropylmethylamine Derivatives as Dopamine D Receptor Partial Agonists: Design, Synthesis, and Biological Evaluation.

J Med Chem 2021 Nov 19. Epub 2021 Nov 19.

iHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Partial agonist activity at the dopamine D receptor (DR) is the primary pharmacological feature of the third-generation antipsychotics─aripiprazole, brexpiprazole, and cariprazine. However, all these drugs share a common phenyl-piperazine moiety as the primary pharmacophore. In this study, we designed and synthesized a series of novel compounds based on the 2-phenylcyclopropylmethylamine (PCPMA) scaffold and studied their pharmacological activity at the DR. A number of potent DR partial agonists were identified through binding affinity screening and functional activity profiling in both G protein and β-arrestin assays. The structure-functional activity relationship results showed that the spacer group is crucial for fine-tuning the intrinsic activity of these compounds. Compounds (+)- and (+)- showed good pharmacokinetic properties and an unexpected selectivity against the serotonin 2A (5-HT) receptor. Preliminary suppressive effects in a mouse hyperlocomotion model proved that these PCPMA-derived DR partial agonists are effective as potential novel antipsychotics.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01327DOI Listing
November 2021

Chronic bisphenol A exposure triggers visual perception dysfunction through impoverished neuronal coding ability in the primary visual cortex.

Arch Toxicol 2021 Nov 16. Epub 2021 Nov 16.

CAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, People's Republic of China.

Contrast perception is a fundamental visual ability that allows us to distinguish objects from the background. However, whether it is perturbed by chronic exposure to environmental xenoestrogen, bisphenol A (BPA), is still elusive. Here, we used adult cats to explore BPA-induced changes in contrast sensitivity (CS) and its underlying neuronal coding mechanism. Behavioral results showed that 14 days of BPA exposure (0.4 mg/kg/day) was sufficient to induce CS declines at the tested spatial frequencies (0.05-2 cycles/deg) in all four cats. Furthermore, based on multi-channel electrophysiological recording and interneuronal correlation analysis, we found that the BPA-exposed cats exhibited an obvious up-regulation in noise correlation in the primary visual cortex (area 17, A17), thus providing a population neuronal coding basis for their perceptual dysfunction. Moreover, single neuron responses in A17 of BPA-exposed cats revealed a slight but marked decrease in CS compared to that of control cats. Additionally, these neuronal responses presented an overt decrease in signal-to-noise ratio, accompanied by increased trial-to-trial response variability (i.e., noise). To some extent, these neuron population and unit dysfunctions in A17 of BPA-exposed cats were attributable to decreased response activity of fast-spiking neurons. Together, our findings demonstrate that chronic BPA exposure restricts contrast perception, in response to impoverished neuronal coding ability in A17.
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http://dx.doi.org/10.1007/s00204-021-03192-zDOI Listing
November 2021

Chronic bisphenol A exposure triggers visual perception dysfunction through impoverished neuronal coding ability in the primary visual cortex.

Arch Toxicol 2021 Nov 16. Epub 2021 Nov 16.

CAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, People's Republic of China.

Contrast perception is a fundamental visual ability that allows us to distinguish objects from the background. However, whether it is perturbed by chronic exposure to environmental xenoestrogen, bisphenol A (BPA), is still elusive. Here, we used adult cats to explore BPA-induced changes in contrast sensitivity (CS) and its underlying neuronal coding mechanism. Behavioral results showed that 14 days of BPA exposure (0.4 mg/kg/day) was sufficient to induce CS declines at the tested spatial frequencies (0.05-2 cycles/deg) in all four cats. Furthermore, based on multi-channel electrophysiological recording and interneuronal correlation analysis, we found that the BPA-exposed cats exhibited an obvious up-regulation in noise correlation in the primary visual cortex (area 17, A17), thus providing a population neuronal coding basis for their perceptual dysfunction. Moreover, single neuron responses in A17 of BPA-exposed cats revealed a slight but marked decrease in CS compared to that of control cats. Additionally, these neuronal responses presented an overt decrease in signal-to-noise ratio, accompanied by increased trial-to-trial response variability (i.e., noise). To some extent, these neuron population and unit dysfunctions in A17 of BPA-exposed cats were attributable to decreased response activity of fast-spiking neurons. Together, our findings demonstrate that chronic BPA exposure restricts contrast perception, in response to impoverished neuronal coding ability in A17.
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http://dx.doi.org/10.1007/s00204-021-03192-zDOI Listing
November 2021

Corrigendum to 'In vitro and in vivo evaluation of antiviral activity of a phenylpropanoid derivative against spring viraemia of carp virus' [Virus Research 291 (2021) 198221].

Virus Res 2022 Jan 12;307:198620. Epub 2021 Nov 12.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo, 315832, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo, 315832, China. Electronic address:

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http://dx.doi.org/10.1016/j.virusres.2021.198620DOI Listing
January 2022

Innovative application of internal carotid artery embolization in salvage endoscopic nasopharyngectomy for recurrent nasopharyngeal carcinoma: A case-matched comparison.

Int Forum Allergy Rhinol 2021 Nov 13. Epub 2021 Nov 13.

ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.

Background: The efficacy and safety of internal carotid artery (ICA) embolization as a treatment strategy in recurrent nasopharyngeal carcinoma (rNPC) patients with tumors invading the ICA remain unclear.

Methods: We enrolled all rNPC patients with tumors invading the ICA, who underwent salvage endoscopic surgery. Using propensity scores to adjust for specific potential prognostic factors, a well-balanced cohort of 42 patients with limited rNPC was formed by matching each patient who underwent ICA embolization (study group) with one who did not (control group). The survival rates and common treatment-related complications were compared between the two groups.

Results: The cohort included patients with the following tumor stages: rT2 (n = 3), rT3 (n = 24), and rT4 (n = 15). During a median follow-up of 15 months (2-63), the 3-year overall survival and progression-free survival rates were significantly higher in the ICA embolization group than in the ICA non-embolization group (72.4% vs. 17.8% and 53.4% vs. 33.0%, respectively; P = 0.022 and P = 0.006, respectively). Additionally, the incidence of treatment-related complications such as nasal obstruction, nasopharyngeal hemorrhage, and nasopharyngeal necrosis was significantly lower in the ICA embolization group than in the non-embolization group (P = 0.001, P = 0.014, and P = 0.038, respectively).

Conclusions: The innovative application of ICA embolization in endoscopic surgery in patients with rNPC invading the ICA significantly improved patient survival and reduced the risk of treatment-related complications. Therefore, this may be a safe and effective method with potential to improve the outcomes in rNPC patients. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/alr.22927DOI Listing
November 2021

MiR-6924-5p-rich exosomes derived from genetically modified Scleraxis-overexpressing PDGFRα(+) BMMSCs as novel nanotherapeutics for treating osteolysis during tendon-bone healing and improving healing strength.

Biomaterials 2021 Nov 5;279:121242. Epub 2021 Nov 5.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, First Affiliated Hospital of Third Military Medical University (Army Medical University), Chongqing, 400000, China. Electronic address:

Osteolysis at the tendon-bone interface can impair pullout strength during tendon-bone healing and lead to surgery failure, but the effects of clinical treatments are not satisfactory. Mesenchymal stem cell (MSC)-derived exosomes have been used as potent and feasible natural nanocarriers for drug delivery and have been proven to enhance tendon-bone healing strength, indicating that MSC-derived exosomes could be a promising therapeutic strategy. In this study, we explored Scleraxis (Scx) dynamically expressed in PDGFRα(+) bone marrow-derived mesenchymal stem cells (BMMSCs) during natural tendon-bone healing. Then, we investigated the role of PDGFRα(+) BMMSCs in tendon-bone healing after Scx overexpression as well as the underlying mechanisms. Our data demonstrated that Scx-overexpressing PDGFRα(+) BMMSCs (BMMSC) could efficiently inhibit peritunnel osteolysis and enhance tendon-bone healing strength by preventing osteoclastogenesis in an exosomes-dependent manner. Exosomal RNA-seq revealed that the abundance of a novel miRNA, miR-6924-5p, was highest among miRNAs. miR-6924-5p could directly inhibit osteoclast formation by binding to the 3'-untranslated regions (3'UTRs) of OCSTAMP and CXCL12. Inhibition of miR-6924-5p expression reversed the prevention of osteoclastogenic differentiation by BMMSC derived exosomes (BMMSC-exos). Local injection of BMMSC-exos or miR-6924-5p dramatically reduced osteoclast formation and improved tendon-bone healing strength. Furthermore, delivery of miR-6924-5p efficiently inhibited the osteoclastogenesis of human monocytes. In brief, our study demonstrates that BMMSC-exos or miR-6924-5p could serve as a potential therapy for the treatment of osteolysis during tendon-bone healing and improve the outcome.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121242DOI Listing
November 2021

A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo.

Nat Biotechnol 2021 Nov 11. Epub 2021 Nov 11.

State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.

Endocannabinoids (eCBs) are retrograde neuromodulators with important functions in a wide range of physiological processes, but their in vivo dynamics remain largely uncharacterized. Here we developed a genetically encoded eCB sensor called GRAB. GRAB consists of a circular-permutated EGFP and the human CB1 cannabinoid receptor, providing cell membrane trafficking, second-resolution kinetics with high specificity for eCBs, and shows a robust fluorescence response at physiological eCB concentrations. Using GRAB, we monitored evoked and spontaneous changes in eCB dynamics in cultured neurons and acute brain slices. We observed spontaneous compartmentalized eCB transients in cultured neurons and eCB transients from single axonal boutons in acute brain slices, suggesting constrained, localized eCB signaling. When GRAB was expressed in the mouse brain, we observed foot shock-elicited and running-triggered eCB signaling in the basolateral amygdala and hippocampus, respectively. In a mouse model of epilepsy, we observed a spreading wave of eCB release that followed a Ca wave through the hippocampus. GRAB is a robust probe for eCB release in vivo.
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http://dx.doi.org/10.1038/s41587-021-01074-4DOI Listing
November 2021

Application value between dynamic electrocardiogram and MSCT myocardial perfusion imaging in the diagnosis of myocardial ischemia in coronary heart disease.

Ann Palliat Med 2021 10;10(10):10720-10725

Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China; Ultrasound in Cardiac Electrophysiology and Biomechanics Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: Dynamic electrocardiogram and multi-spiral computed tomography (MSCT) myocardial perfusion imaging are commonly used in clinical examination methods, which can provide important reference for the diagnosis of myocardial ischemia in coronary heart disease. To analyze and compare the application value of dynamic electrocardiogram and MSCT myocardial perfusion imaging in the diagnosis of myocardial ischemia in coronary heart disease.

Methods: The clinical data of 79 patients diagnosed with coronary heart disease admitted in our hospital from October 2017 to October 2019 were retrospectively collected. Exercise load/rest myocardial perfusion imaging (MPI) confirmed the presence of myocardial ischemia, and the patients were all examined by dynamic electrocardiogram and MSCT. The diagnosis of exercise load/resting MPI was used as a benchmark to compare the sensitivity, specificity, and accuracy of the different tests in the diagnosis of myocardial ischemia in coronary heart disease.

Results: As confirmed by the exercise load/rest MPI, the 79 included patients had myocardial ischemia. Fifty-five cases of myocardial ischemia were detected by dynamic electrocardiogram, of which 33 cases showed horizontal depression of the ST segment. Also, 15 cases exhibited downward oblique depression, and seven cases showed upward oblique depression. MSCT examination showed that 78 patients had myocardial ischemia, of which 74 showed abnormal myocardial perfusion when contracted. Of these patients, 47 showed reversible perfusion filling, 20 exhibited partially reversible perfusion filling, and seven showed fixed perfusion defects. The sensitivity, specificity, and accuracy of dynamic electrocardiogram in the diagnosis of myocardial ischemia in coronary heart disease were 60.76%, 72.15%, 69.62%, respectively, while those of MSCT were 91.14%, 75.94%, and 86.07%, respectively. The sensitivity and accuracy of MSCT examination were significantly higher than those of dynamic electrocardiogram examination (P<0.05), and the specificity was slightly higher than that of dynamic electrocardiogram examination, but this difference was not statistically significant (P>0.05).

Conclusions: Compared to dynamic electrocardiogram examination, MSCT myocardial perfusion imaging has superior sensitivity and accuracy in the diagnosis of myocardial ischemia in coronary heart disease, and can provide a reference for clinical diagnosis and treatment.
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http://dx.doi.org/10.21037/apm-21-2481DOI Listing
October 2021

Core-shell lipoplexes inducing active macropinocytosis promote intranasal delivery of c-Myc siRNA for treatment of glioblastoma.

Acta Biomater 2021 Oct 29. Epub 2021 Oct 29.

Key Laboratory of Smart Drug Delivery, Ministry of Education; Department of Pharmaceutics, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China; The Institutes of Integrative Medicine of Fudan University, Shanghai 200040, China; Shanghai Engineering Research Center of ImmunoTherapeutics, Shanghai 201203, China. Electronic address:

Glioblastoma is the most common and aggressive primary brain tumor, whose malignancy is closely correlated with elevated proto-oncogene c-myc. Intranasal administration emerges as a potential approach to deliver gene into the brain and interfere c-Myc expression. However, powerful permeability in nasal mucosa, selective delivery to glioma and avoidance of premature release during remote transport are imperative to ensure the therapeutic effectiveness. To address the above concerns, herein we constructed a lipoplex based on pre-compression of c-Myc-targeting siRNA (sic-Myc) by octaarginine and subsequent encapsulation by liposome modified with a selected peptide derived from penetratin, named 89WP. It was found that the lipoplex exhibited a stable core-shell structure and could be preferentially internalized along with cell debris by glioma cells via active macropinocytosis. Through this cellular uptake pathway, the lipoplex avoided being entrapped by lysosome and released siRNA in cytoplasm within 4 h, inducing substantial downregulation of c-Myc mRNA and protein expression of glioma cells. Furthermore, due to significantly enhanced permeability in tumor spheroids and nasal mucosa, the lipoplex was competent to deliver more siRNA to orthotopic glioma after intranasal administration, and therefore prolonged the survival time of glioma-bearing mice by inducing apoptosis. STATEMENT OF SIGNIFICANCE: In the present work, a lipoplex was designed to address the unmet demands on intranasal siRNA delivery to the brain for treatment of glioma. First, a powerful peptide was selected to enable the lipoplex to penetrate nasal mucosa. Second, we found the lipoplex could be selectively internalized along with cell debris by glioma cells via active macropinocytosis, and recorded the entire process. This cellular uptake pathway not only prevented the lipoplex being entrapped by lysosome, but also increased distribution of the lipoplex in orthotopic glioma. Third, this lipoplex provided additional protection for siRNA to avoid premature release during transport from nasal to brain. Overall, this lipoplex improved the gene delivery efficiency of intranasal administration and was promising in the perspective of selectively silencing disease-related genes in intracranial tumor.
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http://dx.doi.org/10.1016/j.actbio.2021.10.042DOI Listing
October 2021

Realizing Enhanced Thermoelectric Performance and Hardness in Icosahedral Cu FeS Se with High-Density Twin Boundaries.

Small 2021 Nov 5:e2104592. Epub 2021 Nov 5.

College of Physics, Chongqing University, Chongqing, 401331, P. R. China.

Bornite (Cu FeS ) is an Earth-abundant, nontoxic thermoelectric material. Herein, twin engineering and Se alloying are combined in order to further improve its thermoelectric performance. Cu FeS Se (0 ≤ x ≤ 0.4) icosahedral nanoparticles, containing high-density twin boundaries, have been synthesized by a colloidal method. Spark plasma sintering retains twin boundaries in the pellets sintered from Cu FeS Se colloidal powders. Thermoelectric property measurement demonstrates that alloying Se increases the carrier concentration, leading to much-improved power factor in Se-substituted Cu FeS , for example, 0.84 mW m K at 726 K for Cu FeS Se ; low lattice thermal conductivity is also achieved, due to intrinsic structural complexity, distorted crystal structure, and existing twin boundaries and point defects. As a result, a maximum zT of 0.75 is attained for Cu FeS Se at 726 K, which is about 23% higher than that of Cu FeS and compares favorably to that of reported Cu FeS -based materials. In addition, the Cu FeS Se samples containing twin boundaries also obtain improved hardness compared to the ones fabricated by melting-annealing or ball milling. This work demonstrates an effective twin engineering-composition tuning strategy toward enhanced thermoelectric and mechanical properties of Cu FeS -based materials.
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http://dx.doi.org/10.1002/smll.202104592DOI Listing
November 2021

PIWI-interacting RNA 57125 restrains clear cell renal cell carcinoma metastasis by downregulating CCL3 expression.

Cell Death Discov 2021 Nov 3;7(1):333. Epub 2021 Nov 3.

Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Clear-cell renal cell carcinoma is one of the most common tumors disagnosed, with nearly one third of patients diagnosed with metastatic ccRCC. Although an increasing number of studies has revealed that piwi-interacting RNAs are aberrantly expressed in diverse types of cancers, few of them explored the detailed molecular mechanism of piRNAs in carcinogenesis, particularly in ccRCC. In this study, differentially expressed piRNAs associated with ccRCC were selected by using piRNA-sequencing combined with TCGA data analysis, and piR-57125 was identified. PiR-57125 was found remarkably downregulated in ccRCC samples. Functionally, knockdown of piR-57125 promoted migration and invasion of ccRCC, while overexpression of piR-57125 suppressed ccRCC metastasis. In vivo lung metastasis model also confirmed the same results. CCL3 was identified as the direct target of piR-57125 which could potentially reverse the inhibition effect of piR-57125 in ccRCC metastasis. Further study revealed that piR-57125 modulated ccRCC metastasis through the AKT/ERK pathway. These data indicate that piR-57125 restrains ccRCC metastasis by directly targeting CCL3 and inhibiting the AKT/ERK pathway, and could be a potential therapeutic target for ccRCC.
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http://dx.doi.org/10.1038/s41420-021-00725-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566597PMC
November 2021

Insights into the Ancient Adaptation to Intertidal Environments by Red Algae Based on a Genomic and Multi-Omics Investigation of Neoporphyra haitanensis.

Mol Biol Evol 2021 Nov 3. Epub 2021 Nov 3.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang, 315211, China.

Colonization of land from marine environments was a major transition for biological life on Earth, and intertidal adaptation was a key evolutionary event in the transition from marine- to land-based lifestyles. Multicellular intertidal red algae exhibit the earliest, systematic, and successful adaptation to intertidal environments, with Porphyra sensu lato (Bangiales, Rhodophyta) being a typical example. Here, a chromosome-level 49.67 Mbp genome for Neoporphyra haitanensis comprising 9,496 gene loci is described based on Meta-Hi-C-assisted whole genome assembly, which allowed the isolation of epiphytic bacterial genome sequences from a seaweed genome for the first time. The compact, function-rich N. haitanensis genome revealed that ancestral lineages of red algae share common horizontal gene transfer (HGT) events and close relationships with epiphytic bacterial populations. Specifically, the ancestor of N. haitanensis obtained unique lipoxygenase family genes from bacteria for complex chemical defense, carbonic anhydrases for survival in shell-borne conchocelis lifestyle stages, and numerous genes involved in stress tolerance. Combined proteomic, transcriptomic, and metabolomic analyses revealed complex regulation of rapid responses to intertidal dehydration/rehydration cycling within N. haitanensis. These adaptations include rapid regulation of its photosynthetic system, a readily available capacity to utilize ribosomal stores, increased methylation activity to rapidly synthesize proteins, and a strong anti-oxidation system to dissipate excess redox energy upon exposure to air. These novel insights into the unique adaptations of red algae to intertidal lifestyles inform our understanding of adaptations to intertidal ecosystems and the unique evolutionary steps required for intertidal colonization by biological life.
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http://dx.doi.org/10.1093/molbev/msab315DOI Listing
November 2021

Experimental study on creep properties prediction of reed bales based on SVR and MLP.

Plant Methods 2021 Oct 30;17(1):112. Epub 2021 Oct 30.

Karamay Vocational and Technical College, Xinjiang, China.

Background: Reed has high lignin content, wide distribution and low cost. It is an ideal raw material for replacing wood in the paper industry. Reeds are rich in resources, but the density of reeds is low, leading to high transportation and storage costs. This paper aims to study the compression process of reeds and the creep behaviour of compressed reeds, and provide theoretical guidance for the reed compressor management, bundling equipment and the stability of compressed reed bales.

Results: We have established a multi-layer perceptron network prediction model for the creep characteristics of reeds, and the prediction rate R of this model is greater than 0.997. The constitutive equation, constitutive coefficient and creep quaternary model of the reed creep process were established by using the prediction model. The creep behaviour of the reed bale is positively correlated with the initial maximum compressive stress (σ). During the creep of the reed, the elastic power and the viscous resistance restrict each other. The results show that the proportion of elastic strain in the initial stage is the largest, and gradually decreases to 99.19% over time. The viscoelastic strain increases rapidly with time, then slowly increases, and finally stabilizes to 0.69%, while the plastic strain accounts for the proportion of the total strain. The specific gravity of the reed increases linearly with the increase of creep time, and finally accounts for 0.39%, indicating that as time increases, the damage of the reed's own structure gradually increases.

Conclusions: We studied the relationship between the strain and time of the reed and the strain and creep behaviour of the reed bag under different holding forces under constant force. It is proved that the multi-layer perceptron network is better than the support vector machine regression in predicting the characteristics of reed materials. The three stages of elasticity, viscoelasticity and plasticity in the process of reed creep are analysed in detail. This article opens up a new way for using machine learning methods to predict the mechanical properties of materials. The proposed prediction model provides new ideas for the characterization of material characteristics.
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http://dx.doi.org/10.1186/s13007-021-00814-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556900PMC
October 2021

Quantitative phosphoproteomic analysis reveals chemoresistance-related proteins and signaling pathways induced by rhIL-6 in human osteosarcoma cells.

Cancer Cell Int 2021 Oct 30;21(1):581. Epub 2021 Oct 30.

Orthopedic Oncology Institute, Department of Orthopedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

Background: IL-6 plays a pivotal role in resistance to chemotherapeutics, including lobaplatin. However, the underlying mechanisms are still unclear. This study was to investigate the changes in phosphoproteins and their related signaling pathways in the process of IL-6-induced chemoresistance to lobaplain in osteosarcoma cells.

Methods: We performed a quantitative phosphoproteomic analysis of the response of SaOS-2 osteosarcoma cells to recombinant human IL-6 (rhIL-6) intervention prior to lobaplatin treatment. The cells were divided into the control group (Con), the lobaplatin group (Lob), and the rhIL-6-and-lobaplatin group (IL-6). Three biological replicates of each group were included. The differentially expressed phosphoproteins were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Netphos 3.1 was used for the prediction of kinases, and STRING was used for the visualization of protein-protein interactions. The conserved motifs surrounding the phosphorylated residues were analyzed using the motif-x algorithm. Western blot analysis was performed to verify the differential expression of p-FLNC, its predicted kinase and the related signaling pathway. The results of the bioinformatic analysis were validated by immunohistochemical staining of clinical specimens.

Results: In total, 3373 proteins and 12,183 peptides, including 3232 phosphorylated proteins and 11,358 phosphorylated peptides, were identified and quantified. Twenty-three significantly differentially expressed phosphoproteins were identified in the comparison between the IL-6 and Lob groups, and p-FLNC ranked second among these phosphoproteins. GO and KEGG analyses revealed the pivotal role of mitogen-activated protein kinase signaling in drug resistance induced by rhIL-6. Four motifs, namely, -SPxxK-, -RxxSP-, -SP-, and -SPK-, demonstrated higher expression in the IL-6 group than in the Lob group. The western blot analysis results verified the higher expression of p-FLNC, AKT1, and p-ERK and the lower expression of p-JNK in the IL-6 group than in the Con and Lob groups. The immunohistochemical staining results showed that p-FLNC, AKT1 and p-ERK1/2 were highly expressed in platinum-resistant clinical specimens but weakly expressed in platinum-sensitive specimens, and platinum-resistant osteosarcoma specimens demonstrated weak expression of p-JNK.

Conclusions: This phosphoproteomic study is the first to reveal the signature associated with rhIL-6 intervention before lobaplatin treatment in human osteosarcoma cells. p-FLNC, AKT1, and MAPK signaling contributes to resistance to lobaplatin in osteosarcoma SaOS-2 cells and may represent molecular targets to overcome osteosarcoma chemoresistance.
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http://dx.doi.org/10.1186/s12935-021-02286-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557500PMC
October 2021

Diurnal mood variation symptoms in major depressive disorder associated with evening chronotype: Evidence from a neuroimaging study.

J Affect Disord 2021 Oct 29;298(Pt A):151-159. Epub 2021 Oct 29.

School of Biological Sciences & Medical Engineering, Southeast University, Nanjing 210096, China; Child Development and Learning Science, Key Laboratory of Ministry of Education, 210096, China. Electronic address:

Background: Major depressive disorder (MDD) is often accompanied with classic diurnal mood variation (DMV) symptoms. Patients with DMV symptoms feel a mood improvement and prefer activities at dusk or in the evening, which is consistent with the evening chronotype. Their neural alterations are unclear. In this study, we aimed to explore the neuropathological mechanisms underlying the circadian rhythm of mood and the association with chronotype in MDD.

Methods: A total of 126 depressed patients, including 48 with DMV, 78 without, and 67 age/gender-matched healthy controls (HC) were recruited and underwent a resting-state functional magnetic resonance imaging. Spontaneous neural activity was investigated using amplitude of low-frequency fluctuation (ALFF) and region of interest (ROI)-based functional connectivity (FC) analyses were conducted. The Morningness-Eveningness Questionnaire (MEQ) was utilized to evaluate participant chronotypes and Pearson correlations were calculated between altered ALFF/FC values and MEQ scores in patients with MDD.

Results: Compared with NMV, DMV group exhibited lower MEQ scores, and increased ALFF values in the right orbital superior frontal gyrus (oSFG). We observed that increased FC between the left suprachiasmatic nucleus (SCN) and supramarginal gyrus (SMG). ALFF in the oSFG and FC of rSCN-SMG were negatively correlated with MEQ scores.

Limitation: Some people's chronotypes information is missing.

Conclusion: Patients with DMV tended to be evening type and exhibited abnormal brain functions in frontal lobes. The synergistic changes between frontotemporal lobe, SCN-SMG maybe the characteristic of patients with DMV symptoms.
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http://dx.doi.org/10.1016/j.jad.2021.10.087DOI Listing
October 2021

Association between relative handgrip strength and hypertension in Chinese adults: An analysis of four successive national surveys with 712,442 individuals (2000-2014).

PLoS One 2021 28;16(10):e0258763. Epub 2021 Oct 28.

Physical Education Department-College of Education (CEDU), United Arab Emirates University, Al Ain, Abu Dhabi, United Arab Emirates.

Objectives: This study aimed to identify the 15-year trends of handgrip strength-to-weight (relative HS) and assess the association between relative HS and hypertension among Chinese adults aged 20-69.

Methods: Using a complex, stratified, multistage probability cluster sampling, we analyzed data collected from 712,442 adults aged 20-69 years in four successive national surveys (2000-2014). We used a handheld dynamometer to measure strength and divided by body weight to calculate the relative HS. Blood pressure was recorded with a sphygmomanometer and hypertension was defined as resting systolic blood pressure at least 140 mmHg or diastolic at least 90 mmHg. The Mann-Kendall trend test examined trends in relative HS over time. We also computed odd ratios (OR) and 95% confidence intervals (95% CI) by tertile of relative HS and examined the association between relative HS and hypertension.

Results: The relative HS level decreased with the increase of age in both male and females (p trend <0.001). In each of four surveys, one interquartile decrease in relative HS was associated with an increased risk of hypertension by 44% (OR = 1.44, 95%CI: 1.40-1.47), 58% (OR = 1.58, 95%CI: 1.54-1.62), 48% (OR = 1.48, 95%CI: 1.45-1.52), 43% (OR = 1.43, 95%CI: 1.40-1.47), respectively.

Conclusion: In the Chinese adult population, the relative HS level decreased from 2000 to 2014 across all ages in both males and females. A lower relative HS was associated with a higher risk of hypertension. The findings provided evidence for the association between muscle strength and hypertension in large-scaled population.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258763PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553048PMC
November 2021
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